Plant composition with whitening effect and preparation method thereof
Technical Field
The invention relates to a plant composition with a whitening effect and a preparation method thereof, belonging to the technical field of building construction.
Background
In recent years, along with the improvement of living water, people pay more and more attention to skin care, and natural cosmetics are sought after. However, environmental pollution in the industrial process, daily four-season alternation, temperature change and pressure caused by air conditioning and heating, normal metabolism of human bodies, skin aging and the like cause skin moisture loss, and deep care is performed on the basis of moisturizing of skin in any season, age and skin quality.
Due to the traditional beauty concept of 'one white covering three ugs', Asian women, particularly Chinese women, pursue skin whitening and frenzy thereof. Many products achieve the whitening effect through hydrogen peroxide, hydroquinone, even lead and mercury and compounds thereof, and cause great harm to people.
Green natural products with whitening efficacy are popular with consumers, and substances such as centella, arbutin, kojic acid and the like are widely applied to whitening cosmetics. However, the use range is limited, and a certain number of groups are easy to cause allergic symptoms.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a plant composition with a whitening effect and a preparation method thereof, wherein the saxifrage extract-total flavone in the formula has excellent biological effects of resisting oxidation, inhibiting tyrosinase, resisting bacteria and the like, can be used in the whitening formula, is compounded with a licorice extract, and has synergistic interaction with the licorice extract, so that the whitening composition has the effects of whitening skin, resisting bacteria, diminishing inflammation, removing free radicals and inhibiting melanin formation.
The technical scheme of the invention is as follows:
a plant composition with a whitening effect is prepared from the following raw materials in parts by weight: 15-25 parts of saxifraga stolonifera extract, 15-25 parts of calamus extract, 12-17 parts of rhizoma polygonati extract, 10-15 parts of phellodendron amurense extract, 10-20 parts of radix pseudostellariae extract, 7-12 parts of oldenlandia diffusa extract, 5-10 parts of Chinese pholidota herb extract, 3-6 parts of black tea fungus fermentation liquor and 2-5 parts of patrinia extract.
The invention also comprises a preparation method of the plant composition with the whitening effect, which comprises the following steps:
pouring 15-25 parts by weight of saxifrage extract, 15-25 parts by weight of calamus extract, 12-17 parts by weight of rhizoma polygonati extract, 10-15 parts by weight of phellodendron amurense extract, 10-20 parts by weight of radix pseudostellariae extract, 7-12 parts by weight of oldenlandia diffusa extract, 5-10 parts by weight of pholidota chinensis extract, 3-6 parts by weight of black tea fungus fermentation liquor and 2-5 parts by weight of patrinia extract into a container in sequence, and mixing uniformly to obtain the plant composition.
Further, the saxifrage extract is prepared by the following steps: (1) cleaning and crushing 60-75 parts by weight of saxifrage, dispersing in 120-150 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0-1.2 parts by weight of cellulase into the saxifrage suspension prepared in the step (1) at 40-55 ℃, stirring, and fully reacting for 2-5 hours; (3) adding 0.7-1.0 part by weight of hemicellulase into the suspension prepared in the step (2), stirring, and fully reacting for 2-3 hours; (4) adding 0.6-1.2 parts by weight of pectinase into the suspension prepared in the step (3), stirring, and fully reacting for 1-2 hours; (5) readjusting the temperature of the suspension prepared in the step (4) to 40-50 ℃, adjusting the pH to 4-6, adding 0.6-1.2 parts by weight of mannase, stirring, and fully reacting for 2-3 hours; (6) adding 1.0-1.5 parts by weight of neutral protease, stirring, reacting fully for 1-3 hours, filtering, and removing filter residues; (7) and concentrating the filtrate to 120-150 parts by weight to obtain the saxifrage extract.
Further, the calamus extract is prepared by the following steps: (1) cleaning and crushing 60-100 parts by weight of calamus, stirring and fully mixing 120-200 parts by weight of 75% ethanol mixed water solution at room temperature, fully soaking for 3-5 hours, filtering, and concentrating the filtrate to 30-50 parts by weight; (2) dispersing the calamus filter residue in the step (1) in 150 parts by weight of distilled water, stirring and fully mixing, adding 1.0-1.2 parts by weight of cellulase at 40-55 ℃, stirring, and fully reacting for 2-5 hours; (3) adding 0.7-1.0 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2-3 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 40-50 ℃, adjusting the pH value to 4-6, adding 0.6-1.2 parts by weight of mannase, stirring, and fully reacting for 2-3 h; (5) adding 1.0-1.5 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 1-3 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 120-150 parts by weight, and mixing with the filtrate concentrated solution in the step (1) to obtain 150-200 parts by weight of calamus extract.
Further, the polygonatum sibiricum extract is prepared by the following steps: (1) cleaning and crushing 60-100 parts by weight of rhizoma polygonati, dispersing in 120-200 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0-1.2 parts by weight of hemicellulase into the rhizoma polygonati suspension in the step (1) at the temperature of 40-55 ℃, stirring, and fully reacting for 2-3 hours; (3) adding 0.6-1.2 parts by weight of pectinase into the mixed solution obtained in the step (2), stirring, and fully reacting for 1-2 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 40-50 ℃, adjusting the pH value to 4-6, adding 0.6-1.2 parts by weight of mannase, stirring, and fully reacting for 2-3 h; (5) adding 1.0-1.5 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 1-3 hours, filtering, and removing filter residues; (6) and concentrating the filtrate to 120-150 parts by weight to obtain the polygonatum sibiricum extract.
Further, the phellodendron amurense extract is prepared by the following steps: (1) washing 60-100 parts by weight of phellodendron amurense, crushing, dispersing in 120-200 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0-1.2 parts by weight of cellulase into the phellodendron amurense suspension in the step (1) at the temperature of 40-55 ℃, stirring, and fully reacting for 2-5 hours; (3) adding 0.7-1.0 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2-3 hours; (4) adding 0.6-1.2 parts by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1-2 hours; (5) readjusting the temperature of the mixed solution in the step (4) to 40-50 ℃, adjusting the pH value to 4-6, adding 0.6-1.2 parts by weight of mannase, stirring, and fully reacting for 2-3 h; (6) adding 1.0-1.5 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting fully for 1-3 hours, filtering, and removing filter residues; (7) and concentrating the filtrate to 120-150 parts by weight to obtain the phellodendron amurense extract.
Further, the radix pseudostellariae extract is prepared by the following steps: (1) cleaning and crushing 60-100 parts by weight of radix pseudostellariae, dispersing in 120-200 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0-1.2 parts by weight of cellulase into the radix pseudostellariae suspension liquid in the step (1) at the temperature of 40-55 ℃, stirring, and fully reacting for 2-5 hours; (3) adding 0.7-1.0 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2-3 hours; (4) adding 0.6-1.2 parts by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1-2 hours; (5) readjusting the temperature of the mixed solution in the step (4) to 40-50 ℃, adjusting the pH value to 4-6, adding 0.6-1.2 parts by weight of mannase, stirring, and fully reacting for 2-3 h; (6) adding 1.0-1.5 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting fully for 1-3 hours, filtering, and removing filter residues; (7) and concentrating the filtrate to 120-150 parts by weight to obtain the radix pseudostellariae extract.
Further, the patrinia extract is prepared by the following steps: (1) cleaning and crushing 60-75 parts by weight of herba patriniae, dispersing in 120-200 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0-1.2 parts by weight of cellulase into the herba patriniae suspension in the step (1) at 40-55 ℃, stirring, and fully reacting for 2-5 hours; (3) adding 0.7-1.0 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2-3 hours; (4) adding 0.6-1.2 parts by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1-2 hours; (5) readjusting the temperature of the mixed solution in the step (4) to 40-50 ℃, adjusting the pH value to 4-6, adding 0.6-1.2 parts by weight of mannase, stirring, and fully reacting for 2-3 h; (6) adding 1.0-1.5 parts by weight of neutral protease, stirring, reacting fully for 1-3 hours, filtering, and removing filter residues; (7) and concentrating the filtrate to 100-150 parts by weight to obtain the patrinia extract.
Further, the black tea fungus fermentation liquor is prepared by the following steps: mixing 4-9 parts by weight of black tea, 45-55 parts by weight of sugarcane juice and 900-1200 parts by weight of deionized water, boiling for 100-120 minutes, standing, cooling, filtering, adding deionized water into filtrate to keep the filtrate at 900-1200 parts by weight, uniformly mixing, adding into a container, placing into a high-temperature sterilization container, sterilizing for 20 minutes, cooling to room temperature after sterilization, adding 45-60 parts by weight of black tea fungus mother liquor, sealing with gauze, fermenting for 7 days at normal temperature and normal pressure, and filtering with a screen to obtain the sugarcane black tea fungus fermentation liquor.
Further, the oldenlandia diffusa extract is prepared by the following steps: (1) cleaning and crushing 60-100 parts by weight of spreading hedyotis herb, stirring and fully mixing 120-200 parts by weight of 75% ethanol water solution at room temperature, fully soaking for 3-5 hours, filtering, and concentrating the filtrate to 30-50 parts by weight; (2) dispersing the oldenlandia diffusa filter residues in the step (1) in 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.0-1.2 parts by weight of cellulase at 40-55 ℃, stirring, and fully reacting for 2-5 hours; (3) adding 0.7-1.0 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2-3 hours; (4) adding 1.0-1.5 parts by weight of neutral protease into the mixed solution obtained in the step (3), stirring, reacting fully for 1-3 hours, filtering, and removing filter residues; (5) concentrating the filtrate to 120-150 parts by weight, and mixing with the concentrated filtrate in the step (1) to obtain 150-200 parts by weight of the oldenlandia diffusa extract.
Further, the pholidota chinensis extract is prepared by the following steps: (1) cleaning and crushing 60-100 parts by weight of pholidota chinensis, stirring and fully mixing with 120-200 parts by weight of 75% ethanol water solution at room temperature, fully soaking for 3-5 hours, filtering, and concentrating the filtrate to 30-50 parts by weight; (2) dispersing the pholidota chinensis filter residue in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.0-1.2 parts by weight of amylase at 40-55 ℃, stirring, and fully reacting for 2-5 hours; (3) adding 0.7-1.0 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2-3 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 40-50 ℃, adjusting the pH value to 4-6, adding 0.6-1.2 parts by weight of mannase, stirring, and fully reacting for 2-3 h; (5) adding 1.0-1.5 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 1-3 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 120-150 parts by weight, and mixing with the filtrate concentrated solution in the step (1) to obtain 150-200 parts by weight of the pholidota chinensis extract.
The invention has the following beneficial effects:
the whitening and moisturizing skin care product has the advantages of wide raw material sources, stable performance and the like, has the effects of whitening and moisturizing, can effectively repair damaged epidermal cells, and effectively improves the skin barrier protection function while whitening. The product does not need preservatives such as cason, sodium benzoate, methylisothiazole synephrine, potassium sorbate and the like, can be used for a long time, does not cause side effects to skin, has the advantages of being mild, low in irritation and the like, and can be suitable for sensitive skin.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example one
A preparation method of a plant composition with whitening effect comprises the following steps:
pouring 15 parts by weight of saxifrage extract, 15 parts by weight of calamus extract, 12 parts by weight of rhizoma polygonati extract, 10 parts by weight of phellodendron amurense extract, 10 parts by weight of radix pseudostellariae extract, 7 parts by weight of oldenlandia diffusa extract, 5 parts by weight of pholidota chinensis extract, 3 parts by weight of black tea fungus fermentation liquor and 2 parts by weight of patrinia extract into a container in sequence, and mixing uniformly to obtain the plant composition.
Further, the saxifrage extract is prepared by the following steps: (1) cleaning 60 parts by weight of saxifrage, pulverizing, dispersing in 120 parts by weight of distilled water, and stirring and mixing thoroughly; (2) adding 1.0 part by weight of cellulase into the saxifrage suspension prepared in the step (1) at 40 ℃, stirring, and fully reacting for 2 hours; (3) adding 0.7 part by weight of hemicellulase into the suspension prepared in the step (2), stirring, and fully reacting for 2 hours; (4) adding 0.6 part by weight of pectinase into the suspension prepared in the step (3), stirring, and fully reacting for 1 h; (5) readjusting the temperature of the suspension prepared in the step (4) to 40 ℃, adjusting the pH to 4, adding 0.6 part by weight of mannase, stirring, and fully reacting for 2 hours; (6) adding 1.0 part by weight of neutral protease, stirring, reacting for 1h, filtering, and removing filter residue; (7) concentrating the filtrate to 120 weight parts to obtain herba Saxifragae extract.
Further, the calamus extract is prepared by the following steps: (1) cleaning 60 parts by weight of calamus, crushing, stirring and fully mixing with 120 parts by weight of 75% ethanol mixed water solution at room temperature, fully soaking for 3 hours, filtering, and concentrating the filtrate to 30 parts by weight; (2) dispersing the calamus filter residue in the step (1) in 150 parts by weight of distilled water, stirring and fully mixing, adding 1.0 part by weight of cellulase at 40 ℃, stirring, and fully reacting for 2 hours; (3) adding 0.7 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 40 ℃, adjusting the pH value to 4, adding 0.6 part by weight of mannase, stirring, and fully reacting for 2 hours; (5) adding 1.0 part by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting for 1 hour, filtering, and removing filter residues; (6) concentrating the filtrate to 120 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 150 weight parts of rhizoma Acori Calami extract.
Further, the polygonatum sibiricum extract is prepared by the following steps: (1) cleaning 60 parts by weight of rhizoma polygonati, crushing, dispersing in 120 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0 part by weight of hemicellulase into the polygonatum sibiricum suspension liquid in the step (1) at the temperature of 40 ℃, stirring, and fully reacting for 2 hours; (3) adding 0.6 part by weight of pectinase into the mixed solution obtained in the step (2), stirring, and fully reacting for 1 h; (4) readjusting the temperature of the mixed solution in the step (3) to 40 ℃, adjusting the pH value to 4, adding 0.6 part by weight of mannase, stirring, and fully reacting for 2 hours; (5) adding 1.0 part by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting for 1 hour, filtering, and removing filter residues; (6) concentrating the filtrate to 120 weight parts to obtain rhizoma Polygonati extract.
Further, the phellodendron amurense extract is prepared by the following steps: (1) washing 60 parts by weight of phellodendron amurense, crushing, dispersing in 120 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0 part by weight of cellulase into the phellodendron amurense suspension in the step (1) at 40 ℃, stirring, and fully reacting for 2 hours; (3) adding 0.7 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2 hours; (4) adding 0.6 part by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1 h; (5) readjusting the temperature of the mixed solution in the step (4) to 40 ℃, adjusting the pH value to 4, adding 0.6 part by weight of mannase, stirring, and fully reacting for 2 hours; (6) adding 1.0 part by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting for 1 hour, filtering, and removing filter residues; (7) concentrating the filtrate to 120 weight parts to obtain cortex Phellodendri extract.
Further, the radix pseudostellariae extract is prepared by the following steps: (1) cleaning 60 parts by weight of radix pseudostellariae, crushing, dispersing in 120 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0 part by weight of cellulase into the radix pseudostellariae suspension liquid in the step (1) at 40 ℃, stirring, and fully reacting for 2 hours; (3) adding 0.7 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2 hours; (4) adding 0.6 part by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1 h; (5) readjusting the temperature of the mixed solution in the step (4) to 40 ℃, adjusting the pH value to 4, adding 0.6 part by weight of mannase, stirring, and fully reacting for 2 hours; (6) adding 1.0 part by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting for 1 hour, filtering, and removing filter residues; (7) and concentrating the filtrate to 120 parts by weight to obtain the radix pseudostellariae extract.
Further, the patrinia extract is prepared by the following steps: (1) cleaning and crushing 60 parts by weight of herba patriniae, dispersing in 120 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.0 part by weight of cellulase into the herba patriniae suspension in the step (1) at 40 ℃, stirring, and fully reacting for 2 hours; (3) adding 0.7 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 0.6 part by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1 h; (5) readjusting the temperature of the mixed solution in the step (4) to 40 ℃, adjusting the pH value to 4, adding 0.6 part by weight of mannase, stirring, and fully reacting for 2 hours; (6) adding 1.0 part by weight of neutral protease, stirring, reacting for 1h, filtering, and removing filter residue; (7) concentrating the filtrate to 100 weight parts to obtain herba Patriniae extract.
Further, the black tea fungus fermentation liquor is prepared by the following steps: mixing 4 parts by weight of black tea, 45 parts by weight of sugarcane juice and 900 parts by weight of deionized water, boiling for 100 minutes, standing, cooling, filtering, adding deionized water into the filtrate to keep the filtrate at 900 parts by weight, uniformly mixing, adding into a container, placing into a high-temperature sterilization, sterilizing for 20 minutes, cooling to room temperature after sterilization, adding 45 parts by weight of black tea fungus mother liquor, sealing with gauze, fermenting for 7 days at normal temperature and normal pressure, and filtering with a screen to obtain the sugarcane black tea fungus fermentation liquor.
Further, the oldenlandia diffusa extract is prepared by the following steps: (1) cleaning and crushing 60 parts by weight of spreading hedyotis herb, stirring and fully mixing 120 parts by weight of 75% ethanol water solution at room temperature, fully soaking for 3 hours, filtering, and concentrating the filtrate to 30 parts by weight; (2) dispersing the oldenlandia diffusa filter residue in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.0 part by weight of cellulase at 40 ℃, stirring, and fully reacting for 2 hours; (3) adding 0.7 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2 hours; (4) adding 1.0 part by weight of neutral protease into the mixed solution obtained in the step (3), stirring, reacting for 1 hour, filtering, and removing filter residues; (5) concentrating the filtrate to 120 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 150 weight parts of herba Hedyotidis Diffusae extract.
Further, the pholidota chinensis extract is prepared by the following steps: (1) cleaning and crushing 60 parts by weight of pholidota chinensis, stirring and fully mixing 20 parts by weight of 75% ethanol water solution at room temperature, fully soaking for 3 hours, filtering, and concentrating the filtrate to 30 parts by weight; (2) dispersing the pholidota chinensis residue obtained in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.0 part by weight of amylase at 40 ℃, stirring, and fully reacting for 2 hours; (3) adding 0.7 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 40 ℃, adjusting the pH value to 4, adding 0.6 part by weight of mannase, stirring, and fully reacting for 2 hours; (5) adding 1.0 part by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting for 1 hour, filtering, and removing filter residues; (6) concentrating the filtrate to 120 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 150 weight parts of herba Bulbophylli Inconspicui extract.
Example two
A preparation method of a plant composition with whitening effect comprises the following steps:
pouring 17 parts by weight of saxifrage extract, 21 parts by weight of calamus extract, 15 parts by weight of rhizoma polygonati extract, 12 parts by weight of phellodendron amurense extract, 13 parts by weight of radix pseudostellariae extract, 8 parts by weight of oldenlandia diffusa extract, 7 parts by weight of pholidota chinensis extract, 4 parts by weight of black tea fungus fermentation liquor and 3 parts by weight of patrinia extract into a container in sequence, and mixing uniformly to obtain the plant composition.
Further, the saxifrage extract is prepared by the following steps: (1) cleaning 70 parts by weight of saxifrage, pulverizing, dispersing in 135 parts by weight of distilled water, and stirring and mixing thoroughly; (2) adding 1.05 parts by weight of cellulase into the saxifrage suspension prepared in the step (1) at 52 ℃, stirring, and fully reacting for 3.5 h; (3) adding 0.75 weight part of hemicellulase into the suspension prepared in the step (2), stirring, and fully reacting for 2.25 hours; (4) adding 0.95 weight part of pectinase into the suspension prepared in the step (3), stirring, and fully reacting for 1.75 h; (5) readjusting the temperature of the suspension prepared in the step (4) to 45 ℃, adjusting the pH to 4.8, adding 0.85 part by weight of mannase, stirring, and fully reacting for 2.75 h; (6) adding 1.05 weight parts of neutral protease, stirring, reacting for 2.5h, filtering, and removing filter residue; (7) concentrating the filtrate to 125 weight parts to obtain herba Saxifragae extract.
Further, the calamus extract is prepared by the following steps: (1) cleaning 70 parts by weight of calamus, crushing, stirring and fully mixing with 130 parts by weight of 75% ethanol mixed water solution at room temperature, fully soaking for 4.5 hours, filtering, and concentrating the filtrate to 45 parts by weight; (2) dispersing the calamus filter residue in the step (1) in 150 parts by weight of distilled water, stirring and fully mixing, adding 1.15 parts by weight of cellulase at the temperature of 45 ℃, stirring, and fully reacting for 3 hours; (3) adding 0.85 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.25 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 45 ℃, adjusting the pH value to 5, adding 0.75 part by weight of mannase, stirring, and fully reacting for 2.25 h; (5) adding 1.15 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 2.5 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 130 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 175 weight parts of rhizoma Acori Calami extract.
Further, the polygonatum sibiricum extract is prepared by the following steps: (1) cleaning 85 parts by weight of rhizoma polygonati, crushing, dispersing in 150 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.15 parts by weight of hemicellulase into the polygonatum sibiricum suspension liquid in the step (1) at the temperature of 50 ℃, stirring, and fully reacting for 2.5 hours; (3) adding 1.15 parts by weight of pectinase into the mixed solution obtained in the step (2), stirring, and fully reacting for 1.25 h; (4) readjusting the temperature of the mixed solution in the step (3) to 45 ℃, adjusting the pH value to 5.5, adding 1 part by weight of mannase, stirring, and fully reacting for 2.3 h; (5) adding 1.2 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting for 1.25 hours, filtering, and removing filter residues; (6) and concentrating the filtrate to 125 parts by weight to obtain the polygonatum sibiricum extract.
Further, the phellodendron amurense extract is prepared by the following steps: (1) washing 90 parts by weight of phellodendron amurense, pulverizing, dispersing in 150 parts by weight of distilled water, and stirring and mixing thoroughly; (2) adding 1.1 weight parts of cellulase into the phellodendron amurense suspension in the step (1) at the temperature of 48 ℃, stirring, and fully reacting for 3.5 h; (3) adding 0.85 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.75 hours; (4) adding 0.75 weight part of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.5 h; (5) readjusting the temperature of the mixed solution in the step (4) to 45 ℃, adjusting the pH value to 5, adding 1.1 weight part of mannase, stirring, and fully reacting for 2.5 h; (6) adding 1.25 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting fully for 2 hours, filtering, and removing filter residues; (7) concentrating the filtrate to 130 weight parts to obtain cortex Phellodendri extract.
Further, the radix pseudostellariae extract is prepared by the following steps: (1) cleaning 85 weight parts of radix Pseudostellariae, pulverizing, dispersing in 150 weight parts of distilled water, stirring and mixing thoroughly; (2) adding 1.1 parts by weight of cellulase into the radix pseudostellariae suspension liquid in the step (1) at 48 ℃, stirring, and fully reacting for 3.5 hours; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.25 hours; (4) adding 0.85 weight part of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.5 h; (5) readjusting the temperature of the mixed solution in the step (4) to 45 ℃, adjusting the pH value to 5.5, adding 0.85 part by weight of mannase, stirring, and fully reacting for 2.25 h; (6) adding 1.35 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting for 2.25 hours, filtering, and removing filter residues; (7) and concentrating the filtrate to 135 parts by weight to obtain the radix pseudostellariae extract.
Further, the patrinia extract is prepared by the following steps: (1) cleaning and crushing 70 parts by weight of herba patriniae, dispersing in 140 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.16 weight parts of cellulase into the herba patriniae suspension in the step (1) at 42 ℃, stirring, and fully reacting for 2.25 h; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 0.6 part by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 2 hours; (5) readjusting the temperature of the mixed solution in the step (4) to 40 ℃, adjusting the pH value to 6, adding 0.9 part by weight of mannase, stirring, and fully reacting for 2.25 h; (6) adding 1.35 parts by weight of neutral protease, stirring, reacting for 2 hours, filtering, and removing filter residue; (7) concentrating the filtrate to 120 weight parts to obtain herba Patriniae extract.
Further, the black tea fungus fermentation liquor is prepared by the following steps: mixing 5 parts by weight of black tea, 48 parts by weight of sugarcane juice and 950 parts by weight of deionized water, boiling for 105 minutes, standing, cooling, filtering, adding deionized water into the filtrate to keep the weight of the filtrate at 950 parts, uniformly mixing, adding into a container, placing into a high-temperature sterilization, sterilizing for 20 minutes, cooling to room temperature after sterilization, adding 52 parts by weight of black tea fungus mother liquor, sealing with gauze, fermenting for 7 days at normal temperature and normal pressure, and filtering with a screen to obtain the sugarcane black tea fungus fermentation liquor.
Further, the oldenlandia diffusa extract is prepared by the following steps: (1) cleaning and crushing 90 parts by weight of spreading hedyotis herb, stirring and fully mixing 145 parts by weight of 75% ethanol water solution at room temperature, fully soaking for 3.5 hours, filtering, and concentrating the filtrate to 40 parts by weight; (2) dispersing the oldenlandia diffusa filter residue in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.02 parts by weight of cellulase at 48 ℃, stirring, and fully reacting for 2.25 hours; (3) adding 0.95 weight part of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.25 hours; (4) adding 1.25 parts by weight of neutral protease into the mixed solution obtained in the step (3), stirring, reacting for 2.5 hours, filtering, and removing filter residues; (5) concentrating the filtrate to 130 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 170 weight parts of herba Hedyotidis Diffusae extract.
Further, the pholidota chinensis extract is prepared by the following steps: (1) cleaning 95 parts by weight of pholidota chinensis, crushing, stirring and fully mixing with 150 parts by weight of 75% ethanol aqueous solution at room temperature, fully soaking for 3.25h, filtering, and concentrating the filtrate to 40 parts by weight; (2) dispersing the pholidota chinensis residue obtained in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.05 parts by weight of amylase at the temperature of 45 ℃, stirring, and fully reacting for 3.5 hours; (3) adding 0.88 weight part of hemicellulase into the mixed solution in the step (2), stirring, and fully reacting for 2.2 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 48 ℃, adjusting the pH value to 4.8, adding 0.8 part by weight of mannase, stirring, and fully reacting for 2.5 h; (5) adding 1.35 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 2.2 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 125 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 165 weight parts of herba Bulbophylli Inconspicui extract.
EXAMPLE III
A preparation method of a plant composition with whitening effect comprises the following steps:
sequentially pouring 20 parts by weight of saxifrage extract, 17 parts by weight of calamus extract, 13 parts by weight of rhizoma polygonati extract, 12 parts by weight of phellodendron amurense extract, 15 parts by weight of radix pseudostellariae extract, 8 parts by weight of oldenlandia diffusa extract, 6 parts by weight of pholidota chinensis extract, 5 parts by weight of black tea fungus fermentation liquor and 4 parts by weight of patrinia extract into a container, and uniformly mixing to obtain the plant composition.
Further, the saxifrage extract is prepared by the following steps: (1) cleaning 75 parts by weight of saxifrage, pulverizing, dispersing in 135 parts by weight of distilled water, and stirring and mixing thoroughly; (2) adding 1.12 parts by weight of cellulase into the saxifrage suspension prepared in the step (1) at the temperature of 55 ℃, stirring, and fully reacting for 3.5 hours; (3) adding 0.95 weight part of hemicellulase into the suspension prepared in the step (2), stirring, and fully reacting for 2.2 hours; (4) adding 0.9 weight part of pectinase into the suspension prepared in the step (3), stirring, and fully reacting for 1.25 h; (5) readjusting the temperature of the suspension prepared in the step (4) to 48 ℃, adjusting the pH value to 5.6, adding 1.05 parts by weight of mannase, stirring, and fully reacting for 2.2 h; (6) adding 1.3 parts by weight of neutral protease, stirring, reacting for 2.2h, filtering, and removing filter residue; (7) concentrating the filtrate to 125 weight parts to obtain herba Saxifragae extract.
Further, the calamus extract is prepared by the following steps: (1) cleaning and crushing 75 parts by weight of calamus, stirring and fully mixing 150 parts by weight of 75% ethanol mixed water solution at room temperature, fully soaking for 3.25h, filtering, and concentrating the filtrate to 45 parts by weight; (2) dispersing the calamus filter residue in the step (1) in 150 parts by weight of distilled water, stirring and fully mixing, adding 1.15 parts by weight of cellulase at 45 ℃, stirring, and fully reacting for 2.2 hours; (3) adding 0.85 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.25 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 48 ℃, adjusting the pH value to 4.8, adding 1.15 parts by weight of mannase, stirring, and fully reacting for 2.5 h; (5) adding 1.35 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting for 1.25 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 135 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 180 weight parts of rhizoma Acori Calami extract.
Further, the polygonatum sibiricum extract is prepared by the following steps: (1) cleaning and crushing 90 parts by weight of rhizoma polygonati, dispersing the crushed rhizoma polygonati in 150 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.15 parts by weight of hemicellulase into the polygonatum sibiricum suspension liquid in the step (1) at the temperature of 45 ℃, stirring, and fully reacting for 2.75 hours; (3) adding 0.85 weight part of pectinase into the mixed solution obtained in the step (2), stirring, and fully reacting for 1.5 h; (4) readjusting the temperature of the mixed solution in the step (3) to 48 ℃, adjusting the pH value to 5.2, adding 0.85 part by weight of mannase, stirring, and fully reacting for 2.75 h; (5) adding 1.15 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 2.5 hours, filtering, and removing filter residues; (6) and concentrating the filtrate to 130 parts by weight to obtain the polygonatum sibiricum extract.
Further, the phellodendron amurense extract is prepared by the following steps: (1) cleaning 75 parts by weight of phellodendron amurense, crushing, dispersing in 150 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.15 weight parts of cellulase into the phellodendron amurense suspension in the step (1) at the temperature of 45 ℃, stirring, and fully reacting for 2.25 h; (3) adding 0.85 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 0.85 weight part of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.25 h; (5) readjusting the temperature of the mixed solution in the step (4) to 46 ℃, adjusting the pH value to 5.2, adding 0.9 part by weight of mannase, stirring, and fully reacting for 2.5 h; (6) adding 1.05 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting for 1.5 hours, filtering, and removing filter residues; (7) concentrating the filtrate to 135 weight parts to obtain cortex Phellodendri extract.
Further, the radix pseudostellariae extract is prepared by the following steps: (1) cleaning 75 parts by weight of radix pseudostellariae, crushing, dispersing in 150 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.12 parts by weight of cellulase into the radix pseudostellariae suspension in the step (1) at the temperature of 45 ℃, stirring, and fully reacting for 2.35 hours; (3) adding 0.85 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.25 hours; (4) adding 0.9 weight part of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.25 h; (5) readjusting the temperature of the mixed solution in the step (4) to 46 ℃, adjusting the pH value to 4.6, adding 1 part by weight of mannase, stirring, and fully reacting for 2.25 h; (6) adding 1.08 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting fully for 1.5 hours, filtering, and removing filter residues; (7) and concentrating the filtrate to 140 parts by weight to obtain the radix pseudostellariae extract.
Further, the patrinia extract is prepared by the following steps: (1) cleaning and crushing 72 parts by weight of herba patriniae, dispersing in 150 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.15 parts by weight of cellulase into the herba patriniae suspension in the step (1) at 48 ℃, stirring, and fully reacting for 2.35 h; (3) adding 0.88 weight part of hemicellulase into the mixed solution in the step (2), stirring, and fully reacting for 2.75 hours; (4) adding 1 part by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.25 h; (5) readjusting the temperature of the mixed solution in the step (4) to 42 ℃, adjusting the pH value to 4.9, adding 1.05 parts by weight of mannase, stirring, and fully reacting for 2.75 h; (6) adding 1.08 weight parts of neutral protease, stirring, reacting for 1.5h, filtering, and removing filter residue; (7) concentrating the filtrate to 125 weight parts to obtain herba Patriniae extract.
Further, the black tea fungus fermentation liquor is prepared by the following steps: mixing 7 parts by weight of black tea, 48 parts by weight of sugarcane juice and 1000 parts by weight of deionized water, boiling for 110 minutes, standing, cooling, filtering, adding deionized water into the filtrate to keep the filtrate at 1000 parts by weight, uniformly mixing, adding into a container, placing into a high-temperature sterilization tank, sterilizing for 20 minutes, cooling to room temperature after sterilization, adding 48 parts by weight of black tea fungus mother liquor, sealing with gauze, fermenting for 7 days at normal temperature and normal pressure, and filtering with a screen to obtain the sugarcane black tea fungus fermentation liquor.
Further, the oldenlandia diffusa extract is prepared by the following steps: (1) cleaning 78 parts by weight of oldenlandia diffusa, crushing, stirring and fully mixing 150 parts by weight of 75% ethanol water solution at room temperature, fully soaking for 3.25h, filtering, and concentrating the filtrate to 45 parts by weight; (2) dispersing the oldenlandia diffusa filter residue in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.1 parts by weight of cellulase at 48 ℃, stirring, and fully reacting for 2.35 hours; (3) adding 0.88 weight part of hemicellulase into the mixed solution in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 1.3 parts by weight of neutral protease into the mixed solution obtained in the step (3), stirring, reacting for 2.5 hours, filtering, and removing filter residues; (5) concentrating the filtrate to 135 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 180 weight parts of herba Hedyotidis Diffusae extract.
Further, the pholidota chinensis extract is prepared by the following steps: (1) cleaning 85 parts by weight of pholidota chinensis, crushing, stirring and fully mixing with 150 parts by weight of 75% ethanol aqueous solution at room temperature, fully soaking for 3.25h, filtering, and concentrating the filtrate to 40 parts by weight; (2) dispersing the pholidota chinensis residue obtained in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.12 parts by weight of amylase at 50 ℃, stirring, and fully reacting for 2.5 hours; (3) adding 0.9 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 42 ℃, adjusting the pH value to 4.2, adding 1.05 parts by weight of mannase, stirring, and fully reacting for 2.5 h; (5) adding 1.3 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 2.5 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 135 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 175 weight parts of herba Bulbophylli Inconspicui extract.
Example four
A preparation method of a plant composition with whitening effect comprises the following steps:
sequentially pouring 22 parts by weight of saxifrage extract, 16 parts by weight of calamus extract, 13 parts by weight of rhizoma polygonati extract, 11 parts by weight of phellodendron amurense extract, 13 parts by weight of radix pseudostellariae extract, 10 parts by weight of oldenlandia diffusa extract, 8 parts by weight of pholidota chinensis extract, 4 parts by weight of black tea fungus fermentation liquor and 3 parts by weight of patrinia extract into a container, and uniformly mixing to obtain the plant composition.
Further, the saxifrage extract is prepared by the following steps: (1) cleaning 75 parts by weight of saxifrage, pulverizing, dispersing in 150 parts by weight of distilled water, and stirring and mixing thoroughly; (2) adding 1.2 parts by weight of cellulase into the saxifrage suspension prepared in the step (1) at the temperature of 55 ℃, stirring, and fully reacting for 4 hours; (3) adding 0.9 part by weight of hemicellulase into the suspension prepared in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 0.9 weight part of pectinase into the suspension prepared in the step (3), stirring, and fully reacting for 1.5 h; (5) readjusting the temperature of the suspension prepared in the step (4) to 45 ℃, adjusting the pH value to 5.5, adding 0.85 part by weight of mannase, stirring, and fully reacting for 2.25 h; (6) adding 1.25 parts by weight of neutral protease, stirring, reacting for 2 hours, filtering, and removing filter residue; (7) concentrating the filtrate to 125 weight parts to obtain herba Saxifragae extract.
Further, the calamus extract is prepared by the following steps: (1) cleaning and crushing 80 parts by weight of calamus, stirring and fully mixing 180 parts by weight of 75% ethanol mixed water solution at room temperature, fully soaking for 3.25h, filtering, and concentrating the filtrate to 35 parts by weight; (2) dispersing the calamus filter residue in the step (1) in 150 parts by weight of distilled water, stirring and fully mixing, adding 1.1 parts by weight of cellulase at 50 ℃, stirring, and fully reacting for 3.5 hours; (3) adding 0.9 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.25 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 42 ℃, adjusting the pH value to 4.2, adding 1.05 parts by weight of mannase, stirring, and fully reacting for 2.25 h; (5) adding 1.35 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 2.5 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 125 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 160 weight parts of rhizoma Acori Calami extract.
Further, the polygonatum sibiricum extract is prepared by the following steps: (1) cleaning 75 parts by weight of rhizoma polygonati, crushing, dispersing in 135 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.15 parts by weight of hemicellulase into the rhizoma polygonati suspension in the step (1) at the temperature of 52 ℃, stirring, and fully reacting for 2.25 hours; (3) adding 1.12 parts by weight of pectinase into the mixed solution obtained in the step (2), stirring, and fully reacting for 1.15 h; (4) readjusting the temperature of the mixed solution in the step (3) to 48 ℃, adjusting the pH value to 4.8, adding 1.12 parts by weight of mannase, stirring, and fully reacting for 2.5 h; (5) adding 1.35 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting for 2.75 hours, filtering, and removing filter residues; (6) and concentrating the filtrate to 125 parts by weight to obtain the polygonatum sibiricum extract.
Further, the phellodendron amurense extract is prepared by the following steps: (1) cleaning 85 parts by weight of phellodendron amurense, crushing, dispersing in 135 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.15 weight parts of cellulase into the phellodendron amurense suspension in the step (1) at 52 ℃, stirring, and fully reacting for 3.5 h; (3) adding 0.9 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 1.12 parts by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.2 h; (5) readjusting the temperature of the mixed solution in the step (4) to 48 ℃, adjusting the pH value to 4.8, adding 0.95 weight part of mannase, stirring, and fully reacting for 2.5 h; (6) adding 1.4 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting for 2.75 hours, filtering, and removing filter residues; (7) concentrating the filtrate to 128 weight parts to obtain cortex Phellodendri extract.
Further, the radix pseudostellariae extract is prepared by the following steps: (1) cleaning 95 parts by weight of radix pseudostellariae, crushing, dispersing in 145 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.15 parts by weight of cellulase into the radix pseudostellariae suspension in the step (1) at the temperature of 46 ℃, stirring, and fully reacting for 3.25 h; (3) adding 0.9 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 0.95 weight part of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.25 h; (5) readjusting the temperature of the mixed solution in the step (4) to 48 ℃, adjusting the pH value to 5, adding 0.95 weight part of mannase, stirring, and fully reacting for 2.75 h; (6) adding 1.4 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting for 2.25 hours, filtering, and removing filter residues; (7) and concentrating the filtrate to 138 parts by weight to obtain the radix pseudostellariae extract.
Further, the patrinia extract is prepared by the following steps: (1) cleaning and crushing 70 parts by weight of herba patriniae, dispersing in 145 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.05 parts by weight of cellulase into the herba patriniae suspension in the step (1) at the temperature of 46 ℃, stirring, and fully reacting for 3.25 h; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.75 hours; (4) adding 0.95 weight part of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.75 h; (5) readjusting the temperature of the mixed solution in the step (4) to 44 ℃, adjusting the pH value to 5, adding 0.85 part by weight of mannase, stirring, and fully reacting for 2.5 h; (6) adding 1.4 parts by weight of neutral protease, stirring, reacting for 2.25h, filtering, and removing filter residue; (7) concentrating the filtrate to 120 weight parts to obtain herba Patriniae extract.
Further, the black tea fungus fermentation liquor is prepared by the following steps: mixing 6 parts by weight of black tea, 48 parts by weight of sugarcane juice and 950 parts by weight of deionized water, boiling for 108 minutes, standing, cooling, filtering, adding deionized water into the filtrate to keep the weight of the filtrate at 950 parts, uniformly mixing, adding into a container, placing into a high-temperature sterilization, sterilizing for 20 minutes, cooling to room temperature after sterilization, adding 48 parts by weight of black tea fungus mother liquor, sealing with gauze, fermenting for 7 days at normal temperature and normal pressure, and filtering with a screen to obtain the sugarcane black tea fungus fermentation liquor.
Further, the oldenlandia diffusa extract is prepared by the following steps: (1) cleaning and crushing 90 parts by weight of spreading hedyotis herb, stirring and fully mixing 180 parts by weight of 75% ethanol water solution at room temperature, fully soaking for 3.75 hours, filtering, and concentrating the filtrate to 35 parts by weight; (2) dispersing the oldenlandia diffusa filter residue in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.05 parts by weight of cellulase at 46 ℃, stirring, and fully reacting for 3.75 hours; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.2 hours; (4) adding 1.45 parts by weight of neutral protease into the mixed solution obtained in the step (3), stirring, reacting fully for 2.5 hours, filtering, and removing filter residues; (5) concentrating the filtrate to 135 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 170 weight parts of herba Hedyotidis Diffusae extract.
Further, the pholidota chinensis extract is prepared by the following steps: (1) cleaning 85 parts by weight of pholidota chinensis, crushing, stirring and fully mixing with 180 parts by weight of 75% ethanol aqueous solution at room temperature, fully soaking for 3.75h, filtering, and concentrating the filtrate to 35 parts by weight; (2) dispersing the pholidota chinensis residue obtained in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.12 parts by weight of amylase at 50 ℃, stirring, and fully reacting for 3.75 hours; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.2 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 44 ℃, adjusting the pH value to 5, adding 0.85 part by weight of mannase, stirring, and fully reacting for 2.25 h; (5) adding 1.45 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 2.5 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 142 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 177 weight parts of herba Bulbophylli Inconspicui extract.
EXAMPLE five
A preparation method of a plant composition with whitening effect comprises the following steps:
sequentially pouring 23 parts by weight of saxifrage extract, 17 parts by weight of calamus extract, 15 parts by weight of rhizoma polygonati extract, 12 parts by weight of phellodendron amurense extract, 13 parts by weight of radix pseudostellariae extract, 8 parts by weight of oldenlandia diffusa extract, 6 parts by weight of pholidota chinensis extract, 3.5 parts by weight of black tea fungus fermentation liquor and 2.5 parts by weight of patrinia extract into a container, and uniformly mixing to obtain the plant composition.
Further, the saxifrage extract is prepared by the following steps: (1) cleaning 70 parts by weight of saxifrage, pulverizing, dispersing in 135 parts by weight of distilled water, and stirring and mixing thoroughly; (2) adding 1.15 parts by weight of cellulase into the saxifrage suspension prepared in the step (1) at the temperature of 45 ℃, stirring, and fully reacting for 3.5 hours; (3) adding 0.85 part by weight of hemicellulase into the suspension prepared in the step (2), stirring, and fully reacting for 2.75 hours; (4) adding 1 weight part of pectinase into the suspension prepared in the step (3), stirring, and fully reacting for 1.5 h; (5) readjusting the temperature of the suspension prepared in the step (4) to 48 ℃, adjusting the pH to 5.2, adding 1.1 parts by weight of mannase, stirring, and fully reacting for 2.25 h; (6) adding 1.35 parts by weight of neutral protease, stirring, reacting for 2.25h, filtering, and removing filter residue; (7) concentrating the filtrate to 125 weight parts to obtain herba Saxifragae extract.
Further, the calamus extract is prepared by the following steps: (1) cleaning 85 parts by weight of calamus, crushing, stirring and fully mixing with 175 parts by weight of 75% ethanol mixed water solution at room temperature, fully soaking for 4.75h, filtering, and concentrating the filtrate to 45 parts by weight; (2) dispersing the calamus filter residue in the step (1) in 150 parts by weight of distilled water, stirring and fully mixing, adding 1.05 parts by weight of cellulase at 50 ℃, stirring, and fully reacting for 4.75 hours; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.25 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 47 ℃, adjusting the pH value to 5.5, adding 0.8 part by weight of mannase, stirring, and fully reacting for 2.5 h; (5) adding 1.45 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully, filtering for 2 hours, and removing filter residues; (6) concentrating the filtrate to 135 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 180 weight parts of rhizoma Acori Calami extract.
Further, the polygonatum sibiricum extract is prepared by the following steps: (1) cleaning 85 parts by weight of rhizoma polygonati, crushing, dispersing in 145 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.05 parts by weight of hemicellulase into the polygonatum sibiricum suspension liquid in the step (1) at the temperature of 50 ℃, stirring, and fully reacting for 2.5 hours; (3) adding 0.8 part by weight of pectinase into the mixed solution obtained in the step (2), stirring, and fully reacting for 1.25 h; (4) readjusting the temperature of the mixed solution in the step (3) to 47 ℃, adjusting the pH value to 5.5, adding 0.8 part by weight of mannase, stirring, and fully reacting for 2.25 h; (5) adding 1.25 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 2 hours, filtering, and removing filter residues; (6) and concentrating the filtrate to 137 parts by weight to obtain the sealwort extract.
Further, the phellodendron amurense extract is prepared by the following steps: (1) cleaning 85 parts by weight of phellodendron amurense, crushing, dispersing in 160 parts by weight of distilled water, and stirring and fully mixing; (2) adding 1.15 weight parts of cellulase into the phellodendron amurense suspension in the step (1) at 53 ℃, stirring, and fully reacting for 4.75 h; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.25 hours; (4) adding 0.8 part by weight of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.5 h; (5) readjusting the temperature of the mixed solution in the step (4) to 45 ℃, adjusting the pH value to 5.5, adding 0.88 weight part of mannase, stirring, and fully reacting for 2.5 h; (6) adding 1.25 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting for 1.25 hours, filtering, and removing filter residues; (7) concentrating the filtrate to 130 weight parts to obtain cortex Phellodendri extract.
Further, the radix pseudostellariae extract is prepared by the following steps: (1) cleaning 85 weight parts of radix Pseudostellariae, pulverizing, dispersing in 150 weight parts of distilled water, stirring and mixing thoroughly; (2) adding 1.15 parts by weight of cellulase into the radix pseudostellariae suspension liquid in the step (1) at 48 ℃, stirring, and fully reacting for 2.2 hours; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.75 hours; (4) adding 0.88 weight part of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.5 h; (5) readjusting the temperature of the mixed solution in the step (4) to 45 ℃, adjusting the pH value to 5.2, adding 0.95 part by weight of mannase, stirring, and fully reacting for 2.75 h; (6) adding 1.15 parts by weight of neutral protease into the mixed solution obtained in the step (5), stirring, reacting for 1.25 hours, filtering, and removing filter residues; (7) and concentrating the filtrate to 135 parts by weight to obtain the radix pseudostellariae extract.
Further, the patrinia extract is prepared by the following steps: (1) cleaning 65 weight parts of herba Patriniae, pulverizing, dispersing in 150 weight parts of distilled water, stirring and mixing thoroughly; (2) adding 1.15 parts by weight of cellulase into the herba patriniae suspension in the step (1) at the temperature of 45 ℃, stirring, and fully reacting for 2.5 h; (3) adding 0.75 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 0.85 weight part of pectinase into the mixed solution obtained in the step (3), stirring, and fully reacting for 1.5 h; (5) readjusting the temperature of the mixed solution in the step (4) to 45 ℃, adjusting the pH value to 5, adding 0.85 part by weight of mannase, stirring, and fully reacting for 2.5 h; (6) adding 1.25 parts by weight of neutral protease, stirring, reacting for 2 hours, filtering, and removing filter residue; (7) concentrating the filtrate to 120 weight parts to obtain herba Patriniae extract.
Further, the black tea fungus fermentation liquor is prepared by the following steps: mixing 5 parts by weight of black tea, 48 parts by weight of sugarcane juice and 1000 parts by weight of deionized water, boiling for 110 minutes, standing, cooling, filtering, adding deionized water into the filtrate to keep the filtrate at 1000 parts by weight, uniformly mixing, adding into a container, placing into a high-temperature sterilization tank, sterilizing for 20 minutes, cooling to room temperature after sterilization, adding 55 parts by weight of black tea fungus mother liquor, sealing with gauze, fermenting for 7 days at normal temperature and normal pressure, and filtering with a screen to obtain the sugarcane black tea fungus fermentation liquor.
Further, the oldenlandia diffusa extract is prepared by the following steps: (1) cleaning and crushing 90 parts by weight of spreading hedyotis herb, stirring and fully mixing 150 parts by weight of 75% ethanol water solution at room temperature, fully soaking for 3.5 hours, filtering, and concentrating the filtrate to 40 parts by weight; (2) dispersing the oldenlandia diffusa filter residue in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.1 parts by weight of cellulase at 45 ℃, stirring, and fully reacting for 2.5 hours; (3) adding 0.8 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) adding 1.25 parts by weight of neutral protease into the mixed solution obtained in the step (3), stirring, reacting fully for 2 hours, filtering, and removing filter residues; (5) concentrating the filtrate to 130 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 170 weight parts of herba Hedyotidis Diffusae extract.
Further, the pholidota chinensis extract is prepared by the following steps: (1) cleaning and crushing 80 parts by weight of pholidota chinensis, stirring and fully mixing 150 parts by weight of 75% ethanol aqueous solution at room temperature, fully soaking for 3.5 hours, filtering, and concentrating the filtrate to 40 parts by weight; (2) dispersing the pholidota chinensis residue obtained in the step (1) into 150 parts by weight of aqueous solution, stirring and fully mixing, adding 1.1 parts by weight of amylase at the temperature of 45 ℃, stirring, and fully reacting for 2.5 hours; (3) adding 0.8 part by weight of hemicellulase into the mixed solution obtained in the step (2), stirring, and fully reacting for 2.5 hours; (4) readjusting the temperature of the mixed solution in the step (3) to 45 ℃, adjusting the pH value to 4.5, adding 0.85 part by weight of mannase, stirring, and fully reacting for 2.5 h; (5) adding 1.25 parts by weight of neutral protease into the mixed solution obtained in the step (4), stirring, reacting fully for 2 hours, filtering, and removing filter residues; (6) concentrating the filtrate to 130 weight parts, and mixing with the filtrate concentrate in step (1) to obtain 170 weight parts of herba Bulbophylli Inconspicui extract.
In order to evaluate the using effect of the product, 100 female volunteers 30-45 years old are respectively tested, and the results of the test are evaluated by using a VISIA full-face analyzer and averaging 8 weeks after the volunteers use the product. Table 1 shows the comparison of the use effect of 0.1% arbutin in the examples.
TABLE 1 melanin reduction%
Time/day
|
0
|
7
|
14
|
21
|
28
|
35
|
42
|
49
|
56
|
Control group
|
0
|
-1.8
|
-3.0
|
-4.5
|
-7.2
|
-7.3
|
-7.5
|
-7.8
|
-8.6
|
Example 1
|
0
|
-2.3
|
-5.7
|
-8.9
|
-13.9
|
-14.8
|
-15.0
|
-15.7
|
-16.8
|
Example 2
|
0
|
-2.1
|
-6.1
|
-9.3
|
-14.3
|
-15.6
|
-16.2
|
-16.9
|
-19.8
|
Example 3
|
0
|
-1.9
|
-3.8
|
-7.6
|
-12.7
|
-13.5
|
-14.8
|
-17.3
|
-19.3
|
Example 4
|
0
|
-2.7
|
-5.9
|
-8.6
|
-13.6
|
-14.3
|
-17.2
|
-19.6
|
-21.2
|
Example 5
|
0
|
-3.2
|
-6.5
|
-9.6
|
-14.7
|
-15.9
|
-18.3
|
-21.4
|
-23.1 |
In order to evaluate the irritant effect of the product, 100 female volunteers of 30-45 years old were tested, and the effects of the product such as irritant effect were evaluated by averaging the results with 10% lactic acid as a control, and the results are shown in table 2.
TABLE 3 allergic people of volunteer population%
10% cream
|
62
|
Example 1
|
0
|
Example 2
|
0
|
Example 3
|
0
|
Example 4
|
0
|
Example 5
|
0 |
The data in tables 1 and 2 show that the product disclosed by the invention not only has a good whitening effect, but also has the characteristics of mildness and low irritation, and can be suitable for sensitive skin groups.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.