CN108102950A - A kind of preparation method and application of birds composite probiotics feed - Google Patents
A kind of preparation method and application of birds composite probiotics feed Download PDFInfo
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Abstract
The invention discloses a kind of preparation method and applications of birds composite probiotics feed, the birds composite probiotics feed includes 3 plants of lactobacillus bulgaricus P, systematic name is Lactobacillus bulgaricus, Guangdong Province's Culture Collection is preserved in, deposit number is GDMCC No：60252, preservation address is 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100, and preservation date is on October 20th, 2017.3 plants of lactobacillus bulgaricus P of the present invention can be used as feed addictive, it is added in bird feed, it can promote bird, improve birds trophic level, improve the price of deed, reduce feedstuff-meat ratio, improve culture benefit, and there is inhibitory action to pathogenic bacteria, the use of antibiotic in poultry cultivation can be reduced, improves the immune function and anti-stress ability of birds, reduces the death rate, safe and effective green production birds product is realized, to keeping and promoting the healthy and stable development of poultry cultivation industry to have a good application prospect and market value.
Description
Technical field
The invention belongs to poultry cultivation technical field, it is more particularly related to P-3 plants of lactobacillus bulgaricus and
Its application in birds composite probiotics feed is prepared.
Background technology
As domestic and international market is continuously increased birds (pigeon for meat) demand, China's birds industry forward direction scale, product
Commercialization and mangement enterprization rapidly develop.More than 3,000 ten thousand pairs of whole nation breeding pigeon livestock on hand at present, produces squab about 6.2 hundred million per year, every year with
10%-15% speed develops.Meanwhile poultry disease occurs and popular complication, is come in production due to unreasonable using medicine band
Food-safety problem, people is subject to pay close attention to.
The content of the invention
It is an object of the invention to：The problems such as overcoming abuse of antibiotics present in existing poultry cultivation provides a kind of guarantor
Add P-3 plants of Leah lactobacillus, which can be added to as feed addictive in feed, and having improves birds intestinal health, carries
High birds resistance promotes the advantages of bird.
In order to realize foregoing invention purpose, the present invention provides P-3 plants of lactobacillus bulgaricus, systematic name is
Lactobacillus bulgaricus, are preserved in Guangdong Province's Culture Collection, and deposit number is GDMCC No：
60252, preservation address is 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100, and preservation date is on October 20th, 2017.
Lactobacillus bulgaricus P-3 plants of the present invention can be used for preparing feed addictive.
In order to realize foregoing invention purpose, the present invention also provides a kind of feed addictive, the work containing effective dose
For P-3 plants of the lactobacillus bulgaricus of active ingredient, described lactobacillus bulgaricus P-3 plants, systematic name is
Lactobacillus bulgaricus, are preserved in Guangdong Province's Culture Collection, and deposit number is GDMCC No：
60252, preservation address is 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100, and preservation date is on October 20th, 2017.
The present invention also provides a kind of preparation methods of the bird feed containing compound probiotic, include the following steps：
S1, water are heated to 40~50 DEG C, add in 2~8wt% glucose, 0.1~0.5wt% lactobacillus bulgaricus P-3
Strain, 0.1~0.5wt% saccharomycete and 0.1~0.5wt% bacillus subtilises, stirring and dissolving, culture 18~for 24 hours, it is mixed
Zymotic fluid；
S2, the dregs of beans 10~25% by pulverizing and sieving, corn 10~25% and crack rice 10~30% be mixed 3~
Then 6min accesses mixed fermentation liquid 30~45%, stir 1~5min, then ferment at constant temperature 3 days at 25~35 DEG C, obtain
Fermentation material；
S3,200~800 parts of corn is added in 30~80 parts of fermentation material, 100~300 parts of dregs of beans cracks rice 200~250
Part, 0.1~0.6 part of vitamin, 0.5~3 part of amino acid, 10~30 parts of premix, obtain the bird feed containing compound probiotic；
Wherein, the premix includes mountain flour 30wt%, calcium monohydrogen phosphate 30% and trace element 40%；
Described lactobacillus bulgaricus P-3 plants, systematic name is Lactobacillus bulgaricus, is preserved in wide
East saves Culture Collection, and deposit number is GDMCC No：60252, preservation address is Xianlie Middle Road, Guangzhou City 100
5 building, the building of compound the 59th, preservation date are on October 20th, 2017.
The present invention also provides a kind of bird feeds containing compound probiotic, are prepared by above-mentioned preparation method.
Bird feed of the present invention containing compound probiotic can promote bird, improve bird immunity function.
Preferably, the birds include breeding pigeon, squab, broiler chicken, laying hen.
The application method of bird feed of the present invention containing compound probiotic is：It is added in water per ton described containing compound prebiotic
The bird feed 50kg containing compound probiotic is added in the bird feed 10L or feed per ton of bacterium.
Compared with the prior art, the present invention has the advantages that:
Lactobacillus bulgaricus P-3 plants of the present invention can be used as feed addictive, are added in bird feed, can promote fowl
Class is grown, and improves birds trophic level, improves the price of deed, reduces feedstuff-meat ratio, improves culture benefit, and has inhibition to pathogenic bacteria
Effect, can reduce the use of antibiotic in poultry cultivation, improve the immune function and anti-stress ability of birds, reduce the death rate,
Safe and effective green production birds product is realized, to keeping and promoting the healthy and stable development of poultry cultivation industry that there is good application
Prospect and market value.
Bird feed fermentation process provided by the invention containing compound probiotic is a kind of feasible production fermentation process, can
Fermentation period, raising fermentation material production acid and probiotics viable bacteria amount are reduced, while feeds liquid moist forage than feeding dry feed, favorably
In raising poultry feeding amount.
Culture presevation information:
P-3 plants of lactobacillus bulgaricus, systematic name are Lactobacillus bulgaricus, are preserved in Guangdong Province
Culture Collection, deposit number are GDMCC No：60252, preservation address is the compound of Xianlie Middle Road, Guangzhou City 100
5 building, No. 59 building, preservation date are on October 20th, 2017.
Specific embodiment
In order to become apparent from the purpose of the present invention, technical solution and advantageous effects, with reference to embodiments, to this
Invention is further elaborated.It should be appreciated that the embodiment described in this specification is just for the sake of this hair of explanation
It is bright, be not intended to limit the present invention, parameter, ratio of embodiment etc. can adaptation to local conditions make a choice and substance had no to result
It influences.
Embodiment 1
10 15 age in days health pigeons for meat are chosen, are first put to death normal pigeon for meat, sterile working, with the intestines of oese scraping pigeon for meat
Then road and crop content distinguish continuous streak inoculation to MRS culture mediums with oese, put 36 DEG C in sterile saline
At a temperature of, Anaerobic culturel 48h selects suspicious lactic acid bacteria on plate, purifying, then streak inoculation is in MRS and MC culture mediums, MRS trainings
Base 36 DEG C of Anaerobic culturel 48h, MC culture mediums, 36 DEG C of aerobic culture 48h are supported, after culture, observe colony characteristics.According to《Lactic acid bacteria
Taxonomic identification and experimental method》With《Common bacteria system identification handbook》, to isolated strains carry out morphological feature observation and
Physiology and biochemistry is identified.
The lactic acid bacteria that will be isolated and purified, after MRS plating medium Anaerobic culturels, selection can be such that bromocresol purple is become from purple
The bacterial strain of yellow, Gram's staining and catalase experiment, obtain 1 plant of Gram-positive, catalase negative strain, number is
P-3.MRS culture medium colony characteristics are：Bacterium colony is circular, white, protrusion, the smooth moistening in surface, neat in edge；Choose this bacterium colony into
After row Gram's staining, microscopy, result arranges for thalline in straight or rod-shaped, the Gram-positive of bending, single or paired chain,
Do not produce gemma；MC culture medium colony characteristics：Substantially do not grow.
By this bacterial strain according to《Food national safety standard Food microbe testing reference culture illustrated handbook》In key characteristic
Bio-chemical characteristics are carried out, result is：Sucrose, maltose, gossypose, cellobiose, mannitol, sorbierite, salicylic acid, seven leaves
Glycosides is feminine gender.
According to《Lactic acid bacteria taxonomic identification and experimental method identification》、《Common bacteria system identification handbook》、《Food country
Safety standard Food microbe testing reference culture illustrated handbook》Identify that gained P-3 is：L-2 is lactobacillus bulgaricus.
Embodiment 2
Prepare bird feed：
(1) preparation of zymotic fluid：Water heating temperature adds in the food-grade glucose of 2~8wt% to 40~50 DEG C, adds in
0.1~0.5wt% lactic acid bacterias, 0.1~0.5wt% saccharomycete, 0.1~0.5wt% bacillus, stirring and dissolving, culture 18~
For 24 hours,
(2) culture medium (material) prepares：Dregs of beans, corn, cracking rice pulverizes and sieves, spare.
(3) it is inoculated with：Zymotic fluid PH≤4.0 can be inoculated with after testing, and dregs of beans 10~25%, corn 10 through pulverizing and sieving~
25%th, crack rice and 10~30% stir 3~6min in mixer, zymotic fluid 30~45% obtained by access step (1), stirring 1~
5min is packed after mixing.
(4) ferment：The culture of package encapsulation was sent into fermentation plant, in 25~35 DEG C of ferment at constant temperature 3 days.
(5) fermentation material is checked and accepted：PH≤4.5 after testing, the cfu/g of lactic acid bacteria >=1,000,000,000, bacillus >=1,000,000,000/g, yeast
Bacterium >=50,000,000/g, product are up to standard (for fermentation material).
(6) finished product dispensing：By recipe requirements：200~800 parts of corn, 100~300 parts of dregs of beans, crack rice 200~250 parts,
30~80 parts of fermentation material, 0.1~0.6 part of vitamin, 0.5~3 part of amino acid, premix (mountain flour 30%, calcium monohydrogen phosphate 30%,
40%) 10~30 parts of trace element.
(7) after the assay was approved, pack, storage storage.
Experimental example 1
Feeding experiment：Select 0 age in days of eight storehouses 4000 in pigeon for meat farm to healthy breeding pigeon (per storehouse 500 to) output
Healthy squab is tested.Wherein seven storehouse squabs are test group (I groups), and Article 8 storehouse squab is control group (II group).Raising
Condition is cage, and the daily ration of feeding, drug, drinking-water, health-care sand and other management conditions are identical.Kind pigeon eggs is hatched by hatching room, and 0
Age in days health squab is reclaimed by hatching room and nest breeding pigeon is fed, per 4, nest.I groups routinely Diet；II group is in conventional day
On the basis of grain, increase the bird feed as made from 5% embodiment 2 of total weight；Routinely feeding management during experiment, daily
Twice, free choice feeding, drinking-water, illumination are sunshine, the state of mind of dove and feeding situation are observed and recorded daily, respectively to breast for feeding
Dove is weighed on an empty stomach in 0,7,15,20,25,28,30 age in days mornings.Entire experimental period 30d.The result shows that：It is added in daily ration
Bird feed (II group) squab 7d, 15d, 20d, 25d, 28d, 30d average daily gain is than control group (I made from 5% embodiment 2
Group) 4.20% (P has been respectively increased<0.05), 5.00% (P<0.05), 6.86% (P<0.05), 6.57% (P<0.05)、
75.53% (P<0.01), 18.20% (P<0.01)；Feed-weight ratio is respectively 3.41:1,3.31:1, the price of deed is improved, often
The required feed of 500g weightenings reduces 15.2% (P<0.05).The results show：II group of squab average daily gain and feed-weight ratio
I group is significantly better than, and the squab colour of skin, hair color are more ruddy and bright, dove group's holistic health is in good condition, and the uniformity is preferable, residual
Secondary rate declines 18.2%.
By experimental observation, birds made from 5~8% embodiments 2 are added by weight in the daily ration of pigeon for meat breeding production
Feed has apparent facilitation to the growth of squab, and with the extension of addition biological feedstuff duration, to squab
Growth promoting function is more apparent；The price of deed is also obviously improved, and the required feed that increases weight per 500g reduces more than 15.20%；
Dovecote ammonia odor is also reduced, and breeding environment is improved；Dove group's state of mind is good, and the holistic health level of dove group, which has, substantially to be carried
It rises；The colour of skin of squab, hair color are more ruddy and bright, and the uniformity is preferable；Corresponding drug dose is also reduced.
Experimental example 2
Feeding experiment：For influence of the bird feed to yellow-feather broiler feeding effect made from evaluation embodiment 2, do following
Feeding experiment：It chooses with a collection of 1 age in days health yellow-feather broiler 90, is randomly divided into 3 groups (experiment I groups, experiment II groups, control
Group), every group of 30 chickens, the chicken cultivation of test group and control group adds 5% by weight in same henhouse in test group feed
Bird feed made from embodiment 2, the trophic level and management method of all chickens are consistent.
Routinely feeding management during experiment, twice, free choice feeding, drinking-water, illumination are sunshine for feeding daily, are observed daily
The state of mind of chicken and feeding situation are recorded, chicken is claimed on an empty stomach in 0,7,14,21,28,35,42,56,63 age in days mornings respectively
Weight.Entire experimental period 63d.The result shows that：Bird feed made from 5% embodiment 2 (experiment I, experiment II are added in daily ration
Group), yellow-feather broiler 7d, 14d, 21d, 28d, 35d, 42d, 56d, 63d average daily gain has been respectively increased 17.40% than control group
(P<0.05), 16.02% (P<0.05), 9.01% (P<0.05), 11.08% (P<0.05), 6.78% (P<0.05)、
8.210% (P ＜ 0.05), 9.72% (P ＜ 0.05), 9.32% (P ＜ 0.05), 8.53% (P ＜ 0.05)；Feed-weight ratio is respectively
2.38: 1,2.54: 1, the price of deed is improved, and the required feed that increases weight per 500g reduces 16.3% (P ＜ 0.05).As a result
Display：Experiment I, II group chicken average daily gain and feed-weight ratio are significantly better than control group, and the hair color of chicken is brighter, and chicken group is whole
Health status is good, and chicken manure is dry and comfortable, shapes, and the uniformity is preferable, and inferior rate declines 10.0%.It can be seen that made from embodiment 2
Bird feed can promote yellow-feather broiler growth, put on weight, and ammonia odor also significantly reduces in henhouse, and breeding environment is improved；
Chicken group's state of mind is good, and the holistic health level of chicken group is obviously improved, and test group chicken does not occur in entire feeding period
Intestines problem, the chicken meat product of production safety.
Experimental example 3
Feeding experiment：Choose Guangdong laying hen, three laying hens in Luo Man brown shell layers farm, totally 4457 eggs
Chicken.Wherein two are test group (I groups, 1480 laying hens；II groups, 1493 laying hens), one for control group (III groups, 1484
Laying hen).Control group is feed base daily ration by formula as below：Coordinate by share of weight, 60 parts of corn, 21 parts of dregs of beans, wheat bran
4.5 parts, 1.5 parts of fish meal, 1.5 parts of lard, 6.5 parts of mountain flour, 5 parts of premix, this formula nutritional Indexs measure result：Crude protein
16.5%th, calcium 3.86%, total phosphorus 0.48%, salt 0.37%, moisture 11.5%.Experiment I groups are separately added into 10% with experiment II groups
With 20% embodiment 2 made from bird feed, original formulation feed base daily ration is individually subtracted 10% and 20%, mountain flour added to adjust
Calcium content is to original horizontal.
Test group and control group belong to the chick that same batch is bought and are bred as, and are fed, educated jointly by two keepers
It is young, be bred as, the feeding and management condition in stage of laying eggs it is consistent, test period is 10 days, 17 days, routinely raising pipe during experiment
Reason is fed twice, free choice feeding, drinking-water daily.The state of mind, feeding situation, excrement situation of chicken are observed and recorded daily, accurately
Record qualified egg number, qualified egg size, broken egg number, broken egg size, material quantity, extremely naughty number and the expenses for medicine of each group.It calculates laying rate, lay eggs
Amount, broken rate of eggshell, feedstuff-egg ratio and economic benefit.Result of the test such as table 1.
Table 1
| Group | Control group | Test I groups | Test II groups |
| Test number of days | 17 | 10 | 17 |
| Laying hen number (only) | 1484 | 1480 | 1493 |
| Qualified egg number (piece) | 22983 | 13637 | 23468 |
| Qualified egg size (jin) | 2872.9 | 1718.3 | 2971.0 |
| Material quantity (jin) | 5822 | 3409 | 5809 |
| Broken egg number (piece) | 80 | 34 | 63 |
| Broken egg size (jin) | 9.33 | 3.65 | 7.98 |
| It is dead to wash in a pan number (only) | 2 | 0 | 0 |
| Feedstuff-egg ratio | 2.02 | 1.98 | 1.95 |
| Laying rate (piece) (%) | 91.10 | 92.14 | 92.46 |
| Rate of broken eggs (piece) (%) | 0.35 | 0.25 | 0.27 |
| Rate of broken eggs (jin) (%) | 0.32 | 0.21 | 0.27 |
Result of the test：Test I groups, the feedstuff-egg ratio of II groups is respectively 1.97: 1,1.95: 1；Control group is 2.02: 1；Experiment
Group feed conversion rate it is higher than control group by 5%, 7%.The broken rate of eggshell of experiment I groups, II groups and control group is respectively 0.21%,
0.27% and 0.32%, test group is less than control group.It is respectively 0,0,0.13% to test I groups, II groups and the death and culling rate of control group,
The death and culling rate of experiment is significantly lower than control group.
Test I groups, the formed stools, dry and comfortable of II group laying hens, reduce water just with dyspeptic feed excrement, hence it is evident that reduce extremely
The rate of washing in a pan and rate of broken eggs with the extension of usage time, improve the whole general level of the health, and laying rate improves 1.2~3.5%, expects egg
Than reduction by 0.05~0.15%, increase economic efficiency, bird feed made from the embodiment 2 in addition addition 10% per ton, often
Produce 1kg eggs：Feed cost increases by 0.04 yuan, and egg income increases by 0.04~0.10 yuan, and material depletion is 0.12~0.35 yuan few, economy effect
Benefit dramatically increases.And start to apply probiotic effect more preferable early stage breeding layer chicken, it can effectively reduce body in growth period
The damage of histoorgan, anti-aging speed make body maintain the relatively good general level of the health within growth period, keep high for a long time
Production performance maintains higher laying rate, while can improve immunity of organisms, reduces antibiotic usage, meets people couple
The demand of safety food.
Result of the test is：Test I groups, the formed stools, dry and comfortable of II group laying hens, reduction water just with dyspeptic feed
Excrement, hence it is evident that reduce death and culling rate and rate of broken eggs, with the extension of usage time, improve the whole general level of the health, remarkable in economical benefits.
According to the disclosure and teachings of the above specification, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula carries out appropriate change and modification.Therefore, the invention is not limited in specific embodiment disclosed and described above, to this
Some modifications and changes of invention should also be as falling into the scope of the claims of the present invention.In addition, although this specification
In used some specific terms, but these terms are merely for convenience of description, do not limit the present invention in any way.
Claims (8)
1. P-3 plants of lactobacillus bulgaricus, systematic name is Lactobacillus bulgaricus, and it is micro- to be preserved in Guangdong Province
Biological inoculum collection, deposit number are GDMCC No：60252, preservation address is the compound 59 of Xianlie Middle Road, Guangzhou City 100
5 building, number building, preservation date are on October 20th, 2017.
2. the lactobacillus bulgaricus P-3 plants of application in feed addictive described in claim 1.
A kind of 3. feed addictive, which is characterized in that the lactobacillus bulgaricus P-3 as active component containing effective dose
Strain, described lactobacillus bulgaricus P-3 plants, systematic name is Lactobacillus bulgaricus, is preserved in Guangdong Province
Culture Collection, deposit number are GDMCC No：60252, preservation address is the compound of Xianlie Middle Road, Guangzhou City 100
5 building, No. 59 building, preservation date are on October 20th, 2017.
4. a kind of preparation method of the bird feed containing compound probiotic, which is characterized in that include the following steps：
S1, water are heated to 40~50 DEG C, add in 2~8wt% glucose, P-3 plants of 0.1~0.5wt% lactobacillus bulgaricus,
0.1~0.5wt% saccharomycete and 0.1~0.5wt% bacillus subtilises, stirring and dissolving, culture 18~for 24 hours, obtain mixing hair
Zymotic fluid；
S2,10~25 parts of 10~25 parts of dregs of beans, corn by pulverizing and sieving and 10~30 parts of (by weight) mixings 3 of cracking rice
Then~6min accesses mixed fermentation liquid 30~45 parts (by weight), stir 1~5min, and then constant temperature is sent out at 25~35 DEG C
Ferment 3 days, obtains fermentation material；
S3,200~800 parts of corn, 100~300 parts of dregs of beans, crack rice 200~250 parts, dimension are added in 30~80 parts of fermentation material
Raw 0.1~0.6 part of element, 0.5~3 part of amino acid, premix 10~30 parts (by weight) obtain the birds containing compound probiotic and raise
Material；
Wherein, the premix includes 30 parts of mountain flour, 30 parts of calcium monohydrogen phosphate and trace element 40 parts (by weight)；
Described lactobacillus bulgaricus P-3 plants, systematic name is Lactobacillus bulgaricus, is preserved in Guangdong Province
Culture Collection, deposit number are GDMCC No：60252, preservation address is the compound of Xianlie Middle Road, Guangzhou City 100
5 building, No. 59 building, preservation date are on October 20th, 2017.
A kind of 5. bird feed containing compound probiotic, which is characterized in that the birds containing compound probiotic as described in claim 3
The preparation method of feed is prepared.
6. the bird feed containing compound probiotic described in claim 5 is in promoting bird, improving bird immunity function
Application.
7. the application of the bird feed according to claim 6 containing compound probiotic, which is characterized in that the birds are bag
Include breeding pigeon, squab, broiler chicken, laying hen.
8. the application method of the bird feed containing compound probiotic described in claim 5, which is characterized in that institute is added in water per ton
It states and the bird feed 50kg containing compound probiotic is added in bird feed 10L or feed per ton containing compound probiotic.
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Cited By (2)
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|---|---|---|---|---|
| CN110279817A (en) * | 2019-07-16 | 2019-09-27 | 广西富凤农牧集团有限公司 | A kind of fermented tcm additive and preparation method thereof for preventing and treating fowl respiratory disease |
| CN110279764A (en) * | 2019-07-16 | 2019-09-27 | 广西富凤农牧集团有限公司 | A kind of fermented tcm additive and preparation method thereof improving poultry intestinal tract health |
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