CN108085274A - A kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb and preparation method thereof - Google Patents
A kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb and preparation method thereof Download PDFInfo
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- CN108085274A CN108085274A CN201711340532.XA CN201711340532A CN108085274A CN 108085274 A CN108085274 A CN 108085274A CN 201711340532 A CN201711340532 A CN 201711340532A CN 108085274 A CN108085274 A CN 108085274A
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Abstract
The present invention relates to a kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb and preparation method thereof, the bacterial manure be with Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 made of primary raw material.Agrobacterium rhizogenes (Agrobacterium rhizogenes) plays an important role of to promote plant establishment growth, and then the nutrient of plant is promoted to absorb, there is important influence to growth and development of plants, therefore, use agrobacterium rhizogenes bacterium solution and organic matter composition of the OD values for 0.4 0.6, microbial-bacterial fertilizer is made in common loam, and topdress 23 compound leaf phase corn seedlings, the root growth of corn can be stimulated and increase root absorption area, improve the nutrient absorption efficiency of corn, it has been chiefly to facilitate corn N, the absorption of P, and then promote corn growth, it is low to overcome quality in maize production, the problem of low output.Be conducive to improve the potential economic benefit of crop, be conducive to agricultural production.
Description
Technical field
The present invention relates to a kind of microbial-bacterial fertilizers that corn nutrient is promoted to absorb and preparation method thereof, belong to agricultural technology neck
Domain.
Background technology
Corn (Zea mays L.) belongs to grass family Zea maize seed, scientific name maize, and corn is commonly called as ear of maize, jade
Hay, maize, maize.Corn is the main source of mankind's grain, and corn is known as the laudatory title of life prolonging food, contains abundant albumen
Matter, fat, vitamin, trace element, cellulose and polysaccharide etc., have exploitation high nutrition, high biological function food it is huge
Potentiality.Easy nitrogen stress during corn growth shows as true leaf jaundice, yellow from bottom to top when serious, until top young leaves.In compound leaf
On have parallel continuously or discontinuously iron color spot block along vein, chlorisis is extended from blade tip to base portion or even full leaf is in light yellow, leaf
Arteries and veins also chlorosis, leaf is small and thin, easy to fall off, and stem is elongated.Apply micro- life based on agrobacterium rhizogenes that corn nutrient is promoted to absorb
Object bacterial manure can improve nitrogen content in corn, improve the quality and yield of corn.
Microbial-bacterial fertilizer is according to soil micro-ecosystem principle, Plant Nutrition principle and modern times " organic agriculture "
Basic conception and the microbial-bacterial fertilizer developed contains up to more than ten plants high-efficiency activated beneficial microbe bacterium, suitable for various works
Object uses, and can activate nutrient, improves plant recovery of nutrient, has universal, has broken " specificity " " limitation of common bio-fertilizer
The intrinsic weakness of property " " special fertilizer ", this is that other bio-feritlizers are incomparable.Various types of soil are applicable to, generally
It says, the use that every soil for having plant growth can carry out improvement soil and reduce chemical fertilizer using microbial-bacterial fertilizer
And promote the growth of crop.The plant root that agrobacterium rhizogenes induction generates is flourishing, is conducive to the suction of the minerals such as soil N P
It receives.Ri plasmids can induce conversion plant and generate a large amount of hair roots, promote plant establishment, and flourishing root system is conducive to improve resistance, carry
High crop survival rate.So being conducive to improve the nutrient absorption efficiency of corn using agrobacterium rhizogenes preparation bacterium solution, further carry
High crop quality.
The content of the invention
It, should the object of the present invention is to provide a kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb for above-mentioned phenomenon
Microbial-bacterial fertilizer solves corn using the effect of the distinctive enhancing rootability of agrobacterium rhizogenes and raising nutrient absorption efficiency
Since root system is undeveloped in growth course, survival rate is low caused by rootability is weak, fruit quality is low, low output is asked
Topic, the present invention provides a kind of preparation methods for the microbial-bacterial fertilizer that corn nutrient is promoted to absorb.
The present invention is realized by following technical scheme：
A kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb, the raw material that the microbial-bacterial fertilizer is matched by following component
It is made：
Agrobacterium rhizogenes bacterium solution 30ml
Organic matter composition 800g
Common loam 200g
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601,
R1022, one or more of 15834, strain derive from Chinese microorganism strain collection；
The agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind in animal wastes
It is or several；
The common loam refers to sticking grain in the composition of particle in soil, powder and the moderate soil of grains of sand content.
A kind of preparation method for the microbial-bacterial fertilizer that corn nutrient is promoted to absorb, it is characterised in that：This method includes as follows
Step：
1) preparation of agrobacterium rhizogenes bacterium solution：By Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
One or more of activation to OD values be 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.
2) organic matter composition and common loam are crushed to 40-60 mesh respectively, by organic matter composition and common loam
4:1 is uniformly mixed, and obtains the mixture of organic matter composition and common loam.
3) hair that the organic matter composition prepared in step 2) is prepared with adding in step 1) in the mixture of common loam
Root Agrobacterium bacterium solution uniform stirring adds bacterium solution 30ml with the mixture of common loam per 1kg organic matters composition, obtains microorganism
Bacterial manure.
The present invention has following positive effect：
1st, the microorganism fungus kind that microbial-bacterial fertilizer of the present invention uses for agrobacterium rhizogenes strain A4, R1205, R1000,
R1601, R1022, one or more of 15834, agrobacterium rhizogenes can induce plant and generate hairy root, promote maize root system
Growth, improves the nutrient absorption efficiency of corn, and the growth quality final to corn has important role.
2nd, microbial-bacterial fertilizer of the present invention uses agrobacterium rhizogenes bacterium solution, and organic matter composition and common loam are former material
The advantages of material has cost relatively low, easily obtains, and preparation method is simple, is readily put into production.
3rd, a kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb using the present invention and preparation method thereof is applied to corn
Production, solve corn in growth course due to root system it is undeveloped caused by survival rate is low, nutrient content is low, low output
Problem has huge economic benefit.
Description of the drawings
Fig. 1 is at different number of days (30d, 60d, 90d), does not apply bacterial manure corn (control group) of the present invention and applies the present invention
The nitrogen content variation diagram of bacterial manure corn (experimental group).
Specific embodiment
With reference to case study on implementation, the invention will be further described：
A kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb, the raw material that the microbial-bacterial fertilizer is matched by following component
It is made：
Agrobacterium rhizogenes bacterium solution 30ml
Organic matter composition 800g
Common loam 200g
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601,
R1022, one or more of 15834, strain derive from Chinese microorganism strain collection；
The agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind in animal wastes
It is or several；
The common loam refers to sticking grain in the composition of particle in soil, powder and the moderate soil of grains of sand content.
A kind of preparation method for the microbial-bacterial fertilizer that corn nutrient is promoted to absorb, this method comprises the following steps：
1) preparation of agrobacterium rhizogenes bacterium solution：By Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
One or more of activation to OD values be 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution；
2) organic matter composition and common loam are crushed to 40-60 mesh respectively, by organic matter composition and common loam
4:1 is uniformly mixed, and obtains the mixture of organic matter composition and common loam.
3) hair that the organic matter composition prepared in step 2) is prepared with adding in step 1) in the mixture of common loam
Root Agrobacterium bacterium solution uniform stirring adds bacterium solution 30ml with the mixture of common loam per 1kg organic matters composition, obtains microorganism
Bacterial manure.
Effective viable bacteria >=3 × 10 of the microbial-bacterial fertilizer8cuf/g。
The application process that corn nutrient is promoted to absorb
Corn seed is taken to be seeded in soil, starts to apply fertilizer in corn length to 2-3 piece compound leaf phases, using whole layer placement of fertilizer method by fertilizer
Material is uniformly distributed in soil plough horizon about 1kg/m2.Fertilising in every 30 days once, has apparent well developed root system in plant developmental process,
Vigorous, the characteristics of setting percentage is high is grown, substantially increases crop quality and yield.
Microbial-bacterial fertilizer based on agrobacterium rhizogenes is in maize production application
In buying corn seed 100 on the market, every group each 50, it is divided into control group and experimental group, control group does not apply fertilizer,
Experimental group is applied fertilizer, and is about applied fertilizer 1kg using every ten plants of seedling of whole layer placement of fertilizer method, and fertilising in every 30 days once, is applied fertilizer 3 times, observed and recorded
Reducing sugar.It is as shown in table 1 below to record result：
The plant growing state of 1 different number of days of table
The result shows that the corn for being applied with the microbial-bacterial fertilizer based on agrobacterium rhizogenes has substantially in developmental process
Survival rate it is high, the characteristics of well developed root system, nutrient absorbability is strong, and growth is vigorous, and setting percentage is high, the plant phase less applied fertilizer
Than corn quality and yield increase.Result explanation, a kind of microbial-bacterial fertilizer based on agrobacterium rhizogenes of the invention
The nutrient for promoting corn absorbs, and improves its growth quality, there is important positive effect to agricultural production.
As shown in attached drawing 1：At different number of days (30d, 60d, 90d), not apply bacterial manure corn (control group) and applying bacterium
The nitrogen content variation of fertile corn (experimental group).
As shown in Table 2：At different number of days (30d, 60d, 90d), do not apply bacterial manure corn (control group) and apply bacterial manure jade
Total root long, average root diameter, root surface area, root volume, root quantity and the variation of tip of a root number of rice (experimental group).
Table 2 applies influence of the bacterial manure to Maize Roots
Note：At different number of days (30d, 60d, 90d), do not apply bacterial manure corn (control group) and apply the (experiment of bacterial manure corn
Group) total root long, average root diameter, root surface area, root volume, root quantity and the tip of a root number variation.
As shown in Table 3：At plant ripe (90d), do not apply bacterial manure corn (control group) and apply bacterial manure corn
The comparison of (experimental group) biomass of individual tree, a single plant's output, mass of 1000 kernel and grain number per spike.
Table 3 applies influence of the bacterial manure to corn yield
Note：At plant ripe (90d), do not apply bacterial manure corn (control group) and apply bacterial manure corn (experimental group)
The comparison of biomass of individual tree, a single plant's output, mass of 1000 kernel and grain number per spike.
By kjeldahl apparatus and root scanner etc., the plant for measuring application bacterial manure less applies the corn plant of bacterial manure
The variation of nitrogen content, the variation of root growth and the variation of corn yield in the nutrient content of strain.The data obtained further explanation,
Be applied with has stronger rootability and nutrient absorbability, the plant less applied fertilizer with the corn of bacterial manure in developmental process
It compares, corn quality increases.A kind of microbial-bacterial fertilizer based on agrobacterium rhizogenes of the invention promotes the nutrient of corn
It absorbs, improves the growth quality of corn, there is important positive effect to agricultural production.
Claims (4)
1. a kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb, it is characterised in that：The microbial-bacterial fertilizer is by following component
The raw material of proportioning is made：
Agrobacterium rhizogenes bacterium solution 30ml
Organic matter composition 800g
Common loam 200g
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more of 15834, strain derives from Chinese microorganism strain collection；
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind or several in animal wastes
Kind；
The common loam refers to sticking grain in the composition of particle in soil, powder and the moderate soil of grains of sand content.
A kind of 2. microbial-bacterial fertilizer that corn nutrient is promoted to absorb according to claim 1, which is characterized in that the hair
Root Agrobacterium bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
3. a kind of preparation method of microbial-bacterial fertilizer that corn nutrient is promoted to absorb according to claim 1, feature exist
In：This method comprises the following steps：
1) preparation of agrobacterium rhizogenes bacterium solution, by one in Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
Kind or several activation to OD values are 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.
2) organic matter composition and common loam are crushed to 40-60 mesh respectively, by organic matter composition and common loam 4:1 is mixed
Close the mixture for uniformly, obtaining organic matter composition and common loam.
3) the root of hair agriculture that the organic matter composition prepared in step 2) is prepared with adding in step 1) in the mixture of common loam
Bacillus bacterium solution uniform stirring adds bacterium solution 30ml with the mixture of common loam per 1kg organic matters composition, obtains microbial bacteria
Fertilizer.
4. a kind of preparation method of microbial-bacterial fertilizer that corn nutrient is promoted to absorb according to claim 3, feature exist
In effective viable bacteria >=3 × 10 of the microbial-bacterial fertilizer8cuf/g。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711340532.XA CN108085274A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb and preparation method thereof |
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| CN201711340532.XA CN108085274A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer that corn nutrient is promoted to absorb and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109983996A (en) * | 2019-03-29 | 2019-07-09 | 南宁学院 | A kind of cultural method of richness calcium iron corn |
Citations (2)
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| US4517008A (en) * | 1981-05-04 | 1985-05-14 | Research And Development Institute, Inc. At Montana State University | Compositions containing and methods of use of an infectivity-cured Hr plasmid-bearing microorganism |
| CN107278590A (en) * | 2017-07-28 | 2017-10-24 | 周运德 | Improve ginsenoside Rb in cultivation field seven1The method of content |
-
2017
- 2017-12-14 CN CN201711340532.XA patent/CN108085274A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4517008A (en) * | 1981-05-04 | 1985-05-14 | Research And Development Institute, Inc. At Montana State University | Compositions containing and methods of use of an infectivity-cured Hr plasmid-bearing microorganism |
| CN107278590A (en) * | 2017-07-28 | 2017-10-24 | 周运德 | Improve ginsenoside Rb in cultivation field seven1The method of content |
Non-Patent Citations (5)
| Title |
|---|
| GISELA HÖFLICH等: "INFLUENCE OF ASSOCIATIVE RHIZOSPHERE BACTERIA ON NUTRIENT UP-TAKE AND YIELD OF MAIZE", 《ARCHIVES OF AGRONOMY AND SOIL SCIENCE》 * |
| 吕大炜等主编: "《地下乌金 煤》", 30 June 2016, 山东科学技术出版社 * |
| 王敬文等: "发根农杆菌促进黑荆树切根幼苗生根", 《林业科学研究》 * |
| 陈顺增主编: "《土地管理知识辞典》", 30 June 1991, 中国经济出版社 * |
| 黄友谊编: "《茶叶微生物产品学》", 31 August 2017, 中国轻工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109983996A (en) * | 2019-03-29 | 2019-07-09 | 南宁学院 | A kind of cultural method of richness calcium iron corn |
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