CN108083908A - A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof - Google Patents
A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof Download PDFInfo
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- CN108083908A CN108083908A CN201711415683.7A CN201711415683A CN108083908A CN 108083908 A CN108083908 A CN 108083908A CN 201711415683 A CN201711415683 A CN 201711415683A CN 108083908 A CN108083908 A CN 108083908A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C1/00—Ammonium nitrate fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The present invention is suitable for alkaline land improving technical field, provides a kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof, includes the following steps：The first step：The raw material of microbial-bacterial fertilizer is chosen, including：Charcoal, poly- maleic acid, charcoal base ammonium nitrate, mixed microorganism microbial inoculum；And charcoal, poly- maleic acid, charcoal base ammonium nitrate are uniformly mixed in agitator tank successively；The mixed microorganism microbial inoculum includes saccharomycete, photosynthetic bacteria, bacillus subtilis, rhizobium, trichoderma viride and bacillus；Second step：Culture medium is made, and strain expands culture in suitable culture medium by the mixed microorganism microbial inoculum；3rd step：Strain is expanded into the mixed bacteria and charcoal after culture according to mixing, and the agricultural crop straw for adding crushing in the mixture is mixed with methane slag.Whereby, the present invention makes it improve salt-soda soil, is more conducive to the needs for meeting Planting Crops by the way that charcoal is mixed with the raw material of microbial-bacterial fertilizer.
Description
Technical field
The present invention relates to alkaline land improving technical field more particularly to a kind of microbial-bacterial fertilizers and its system containing charcoal
Make method.
Background technology
Biological carbon, be by organic waste, such as animal wastes, animal bone, plant roots and stems, the processing such as sawdust and Wheat Straw and
Into a kind of porous carbon.These organic wastes for forming biological carbon are referred to as " biomass ".Many scientists are " black titled with biological carbon
The good reputation of color gold ".
Charcoal is not general charcoal, is a kind of extremely abundant charcoal of carbon content.It is under low-oxygen environment, is passed through
Timber, grass, cornstalk or other Wastes of Crops are carbonized by Pintsch process.It is this to be formed by plant, using fix carbon as
The charcoal of purpose is known as " charcoal " by scientists.Its theoretical foundation is:Biomass, whether plant or animal,
Do not have to burn in the case of oxygen, charcoal can be formed.
Charcoal is a kind of biomass crossed by Pintsch process " processing ".Cracking process can be not only generated for the energy
The gas of production also has a kind of stable form -- the charcoal of carbon, and charcoal is embedded in underground, and whole process is " carbon negativity "
(carbon negative)。
Charcoal is almost pure carbon, can have hundreds of will not disappear to more than one thousand years after burying to underground, equal to carbon sequestration into
Soil.Charcoal is rich in micropore, can not only supplement the content of organics of soil, can also effectively preserve moisture and support
Material, increases soil fertility.In fact, why black is mostly presented in fertile soil, it is exactly because phosphorus content is high.English
State's environmental protection great master's James's Lovelock claims, and charcoal is the unique hope for mitigating Disastrous climate variation.Researcher
It represents, charcoal can also improve agricultural productivity, reduce the demand to the intensive fertilizer of carbon.Ten partial volume of pore space structure of charcoal particle
Easily aggregation nutriment and beneficial microbe so that soil becomes fertile, beneficial to plant growth, allows agriculture while realizing volume increase
Industry has more continuation.
The loss of rainwater when the superpower adsorptivity of charcoal can adsorb the portion of water in air and reduce rainfall, most
The arable layer largely rainwater being adsorbed onto where it, the growth for crop need so that the soil of water shortage arid area can
Vegetation is grown, prevents desertification.
The content of the invention
A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof, includes the following steps：
The first step：The raw material of microbial-bacterial fertilizer is chosen, in parts by weight, including：
25~35 parts of charcoal, 5~15 parts of poly- maleic acid, 4~10 parts of charcoal base ammonium nitrate, mixed microorganism microbial inoculum 5
~10 parts；Charcoal, poly- maleic acid, charcoal base ammonium nitrate are uniformly mixed in agitator tank with mixed microorganism microbial inoculum successively
50~100 minutes；The mixed microorganism microbial inoculum includes bacillus megaterium, bacillus subtilis, photosynthetic bacteria, withered grass gemma
Bacillus, rhizobium and trichoderma viride bacterium；
Second step：Culture medium is made, and strain expands culture in suitable culture medium by the mixed microorganism microbial inoculum,
The bacterial strain quantity survived after detection culture, when amount of survival >=1.5~3.5 × 10 of bacterial strain8During/ml, stop culture；
3rd step：Strain is expanded into the mixed bacteria after culture with charcoal according to 1:10~15 ratio uniform mixes
It closes, and adds the agricultural crop straw and methane slag of crushing in the mixture, the mixed proportion of the agricultural crop straw and methane slag
For 3:1.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, in second step：According to weight
Number meter is measured, the culture medium includes：4~8 parts of yeast extract, 18~22 parts of peptone adjust 18~22 parts and 500 parts of matrix
Deionized water adjusts pH value 6.8~7.2；
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, in the third step, the agriculture
Crop material includes the mixture of maize straw, wheat stalk, soybean stalk and broomcorn straw.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, the maize straw, wheat
The mixed proportion of stalk, soybean stalk and broomcorn straw by weight is 1：2：2：1.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof expands in the strain and cultivates
In, it would be desirable to microorganism fungus kind individually expand culture, the wherein cultivation temperature of bacillus megaterium in suitable culture medium
For 27~29 DEG C, incubation time is 46~50h；The cultivation temperature of bacillus subtilis is 20~35 DEG C, incubation time for 48~
60h；Photosynthetic bacteria cultivation temperature is 34~40 DEG C, and incubation time is 32~50h；Rhizobium cultivation temperature is 27~35 DEG C, training
It is 30~50h to support the time；Trichoderma viride cultivation temperature is 20~35 DEG C, and incubation time is 3~5 days；The culture of bacillus
25~30 DEG C of temperature, incubation time are 3~5 days；It is more uniform in order to which strain is made to be mixed with culture medium, expand in strain and train
It is rotated during supporting with agitator tank with the rotating speed of 18~40r/min.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, in the 3rd step, the farming
The fineness of object stalk is 200~400 mesh.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with specific embodiment, to this
Invention is further elaborated, it should be understood that the specific embodiments described herein are merely illustrative of the present invention, not
For limiting the present invention.
Embodiment 1
A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof, includes the following steps：
The first step：The raw material of microbial-bacterial fertilizer is chosen, in parts by weight, including：
25 parts of charcoal, 5 parts of poly- maleic acid, 4 parts of charcoal base ammonium nitrate, 5 parts of mixed microorganism microbial inoculum；Successively by biology
Charcoal, poly- maleic acid, charcoal base ammonium nitrate uniformly mix 50 minutes in agitator tank with mixed microorganism microbial inoculum；The mixing is micro-
Bacteria agent includes bacillus megaterium, bacillus subtilis, photosynthetic bacteria, bacillus subtilis, rhizobium and Trichoderma viride
Bacterium bacterium；
Second step：Culture medium is made, and strain expands culture in suitable culture medium by the mixed microorganism microbial inoculum,
The bacterial strain quantity survived after detection culture, when amount of survival >=1.5 × 10 of bacterial strain8During/ml, stop culture；
3rd step：Strain is expanded into the mixed bacteria after culture with charcoal according to 1:10 ratio uniform mixing,
And the agricultural crop straw 300kg for adding crushing in the mixture is uniformly mixed with methane slag 100kg.
Preferably, in second step：In parts by weight, the culture medium includes：4 parts of yeast extract, peptone 18
Part, 18 parts and 500 parts deionized waters of matrix are adjusted, adjust pH value to 6.8；
Preferably, in the third step, the maize straw, wheat stalk, soybean stalk and broomcorn straw are distinguished by weight
For 100kg, 200kg, 200kg and 100kg.
Preferably, the strain expand culture in, it would be desirable to microorganism fungus kind individually expand in suitable culture medium
Big culture, the wherein cultivation temperature of bacillus megaterium are 27~29 DEG C, incubation time 46h；The culture of bacillus subtilis
Temperature is 20~35 DEG C, incubation time 48h；Photosynthetic bacteria cultivation temperature is 34~40 DEG C, incubation time 32h；Rhizobium
Cultivation temperature is 27~35 DEG C, incubation time 30h；Trichoderma viride cultivation temperature is 20~35 DEG C, and incubation time is 3 days；
25~30 DEG C of the cultivation temperature of bacillus, incubation time are 3 days；And strain expands in incubation with agitator tank with 18r/
The rotating speed rotation of min.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, in the 3rd step, the farming
The fineness of object stalk is 200 mesh.
Embodiment 2
A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof, includes the following steps：
The first step：The raw material of microbial-bacterial fertilizer is chosen, in parts by weight, including：
30 parts of charcoal, 10 parts of poly- maleic acid, 8 parts of charcoal base ammonium nitrate, 6 parts of mixed microorganism microbial inoculum；It successively will be raw
Object charcoal, poly- maleic acid, charcoal base ammonium nitrate uniformly mix 80 minutes in agitator tank with mixed microorganism microbial inoculum；The mixing
Microbial bacterial agent includes bacillus megaterium, bacillus subtilis, photosynthetic bacteria, bacillus subtilis, rhizobium and green wood
Mould bacterium；
Second step：Culture medium is made, and strain expands culture in suitable culture medium by the mixed microorganism microbial inoculum,
The bacterial strain quantity survived after detection culture, when amount of survival >=2.0 × 10 of bacterial strain8During/ml, stop culture；
3rd step：Strain is expanded into the mixed bacteria after culture with charcoal according to 1:12 ratio uniform mixing,
And the agricultural crop straw 450kg and methane slag 50kg of crushing are added in the mixture and is uniformly mixed.
Preferably, in second step：In parts by weight, the culture medium includes：6 parts of yeast extract, peptone 20
Part, 20 parts and 500 parts deionized waters of matrix are adjusted, adjust pH value 7.2；
Preferably, in the third step, the agricultural crop straw includes maize straw, wheat stalk, soybean stalk and sorghum
The mixture of stalk.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, the maize straw, wheat
The mixed proportion of stalk, soybean stalk and broomcorn straw by weight is 1：2：2：1.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof expands in the strain and cultivates
In, it would be desirable to microorganism fungus kind individually expand culture, the wherein cultivation temperature of bacillus megaterium in suitable culture medium
For 27~29 DEG C, incubation time 48h；The cultivation temperature of bacillus subtilis is 20~35 DEG C, incubation time 52h；It is photosynthetic
Bacteria Culture temperature is 34~40 DEG C, incubation time 45h；Rhizobium cultivation temperature is 27~35 DEG C, incubation time 40h；
Trichoderma viride cultivation temperature is 20~35 DEG C, and incubation time is 4 days；25~30 DEG C of the cultivation temperature of bacillus, during culture
Between be 4 days；And strain expands in incubation with agitator tank with the rotating speed rotation of 30r/min.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, in the 3rd step, the farming
The fineness of object stalk is 350 mesh.
Embodiment 3
A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof, includes the following steps：
The first step：The raw material of microbial-bacterial fertilizer is chosen, in parts by weight, including：
25~35 parts of charcoal, 15 parts of poly- maleic acid, 10 parts of charcoal base ammonium nitrate, 10 parts of mixed microorganism microbial inoculum；According to
It is secondary uniformly to mix charcoal, poly- maleic acid, charcoal base ammonium nitrate 100 minutes with mixed microorganism microbial inoculum in agitator tank；
The mixed microorganism microbial inoculum includes bacillus megaterium, bacillus subtilis, photosynthetic bacteria, bacillus subtilis, rhizobium
With trichoderma viride bacterium；
Second step：Culture medium is made, and strain expands culture in suitable culture medium by the mixed microorganism microbial inoculum,
The bacterial strain quantity survived after detection culture, when amount of survival >=3.5 × 10 of bacterial strain8During/ml, stop culture；
3rd step：Strain is expanded into the mixed bacteria after culture with charcoal according to 1:15 ratio uniform mixing,
And the agricultural crop straw 600kg for adding crushing in the mixture is uniformly mixed with methane slag 200kg.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, in second step：According to weight
Number meter is measured, the culture medium includes：8 parts of yeast extract, 22 parts of peptone adjust 22 parts and 500 parts deionized waters of matrix, pH
Value 7.0；
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, in the third step, the agriculture
Crop material includes the mixture of maize straw, wheat stalk, soybean stalk and broomcorn straw.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, the maize straw, wheat
Stalk, soybean stalk and broomcorn straw mixed weight are respectively 100kg, 200kg, 200kg and 100kg.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof expands in the strain and cultivates
In, it would be desirable to microorganism fungus kind individually expand culture, the wherein cultivation temperature of bacillus megaterium in suitable culture medium
For 27~29 DEG C, incubation time 50h；The cultivation temperature of bacillus subtilis is 20~35 DEG C, incubation time 60h；It is photosynthetic
Bacteria Culture temperature is 34~40 DEG C, incubation time 50h；Rhizobium cultivation temperature is 27~35 DEG C, incubation time 50h；
Trichoderma viride cultivation temperature is 20~35 DEG C, and incubation time is 5 days；25~30 DEG C of the cultivation temperature of bacillus, during culture
Between be 5 days；And strain expands in incubation with agitator tank with the rotating speed rotation of 30r/min.
Microbial-bacterial fertilizer according to the present invention containing charcoal and preparation method thereof, in the 3rd step, the farming
The fineness of object stalk is 400 mesh.
Beneficial effects of the present invention：
It understands in conjunction with specific embodiments, when the quantity of bacterial strain is more, chlorate, sulfate lane degree and tune in soil is reduced
It is more apparent to save the effect of pH value, therefore in implementing the present invention, it may, bacterial strain quantity should be expanded as far as possible, has reached more preferable
Improved effect.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention, ripe
It knows those skilled in the art and makes various corresponding changes and deformation, but these corresponding changes and change in accordance with the present invention
Shape should all belong to the protection domain of appended claims of the invention.
Claims (6)
1. a kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof, which is characterized in that include the following steps：
The first step：The raw material of microbial-bacterial fertilizer is chosen, in parts by weight, including：
25~35 parts of charcoal, 5~15 parts of poly- maleic acid, 4~10 parts of charcoal base ammonium nitrate, mixed microorganism microbial inoculum 5~10
Part；Charcoal, poly- maleic acid, charcoal base ammonium nitrate uniformly mixes in agitator tank with mixed microorganism microbial inoculum to 50 successively~
100 minutes；The mixed microorganism microbial inoculum includes bacillus megaterium, bacillus subtilis, photosynthetic bacteria, rhizobium and green
Trichoderma bacterium；
Second step：Culture medium is made, and strain expands culture, detection in suitable culture medium by the mixed microorganism microbial inoculum
The bacterial strain quantity survived after culture, when amount of survival >=1.5~3.5 × 10 of bacterial strain8During/ml, stop culture；
3rd step：Strain is expanded into the mixed bacteria after culture with charcoal according to 1:10~15 ratio uniform mixing, and
Add the agricultural crop straw and methane slag of crushing in mixture, the mixed proportion of the agricultural crop straw and methane slag is 3:1.
2. microbial-bacterial fertilizer according to claim 1 containing charcoal and preparation method thereof, which is characterized in that second
In step：In parts by weight, the culture medium includes：4~8 parts of yeast extract, 18~22 parts of peptone, adjusting matrix 18~
22 parts and 500 parts of deionized waters adjust pH value 6.8~7.2.
3. microbial-bacterial fertilizer according to claim 1 containing charcoal and preparation method thereof, which is characterized in that the 3rd
In step, the agricultural crop straw includes the mixture of maize straw, wheat stalk, soybean stalk and broomcorn straw.
4. microbial-bacterial fertilizer according to claim 1 containing charcoal and preparation method thereof, which is characterized in that the jade
Rice stalk, wheat stalk, the mixed proportion of soybean stalk and broomcorn straw by weight are 1：2：2：1.
5. microbial-bacterial fertilizer according to claim 1 containing charcoal and preparation method thereof, which is characterized in that described
Strain expand culture in, it would be desirable to microorganism fungus kind in suitable culture medium individually expand culture, wherein huge gemma bar
The cultivation temperature of bacterium is 27~29 DEG C, and incubation time is 46~50h；The cultivation temperature of bacillus subtilis is 20~35 DEG C, training
It is 48~60h to support the time；Photosynthetic bacteria cultivation temperature is 34~40 DEG C, and incubation time is 32~50h；Rhizobium cultivation temperature is
27~35 DEG C, incubation time is 30~50h；Trichoderma viride cultivation temperature is 20~35 DEG C, and incubation time is 3~5 days；Gemma
25~30 DEG C of the cultivation temperature of bacillus, incubation time are 3~5 days；It is more uniform in order to which strain is made to be mixed with culture medium,
Strain expands in incubation to be rotated with agitator tank with the rotating speed of 18~40r/min.
6. microbial-bacterial fertilizer according to claim 1 containing charcoal and preparation method thereof, which is characterized in that the 3rd step
In, the fineness of the agricultural crop straw is 200~400 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711415683.7A CN108083908A (en) | 2017-12-25 | 2017-12-25 | A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711415683.7A CN108083908A (en) | 2017-12-25 | 2017-12-25 | A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof |
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| CN108083908A true CN108083908A (en) | 2018-05-29 |
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| CN201711415683.7A Withdrawn CN108083908A (en) | 2017-12-25 | 2017-12-25 | A kind of microbial-bacterial fertilizer containing charcoal and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109320355A (en) * | 2018-11-25 | 2019-02-12 | 江苏省农业科学院 | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application |
| CN113636895A (en) * | 2021-08-03 | 2021-11-12 | 沧州市农林科学院 | Microbial organic bacterial fertilizer, mixing and stirring device thereof and use method |
| EP3932896A1 (en) * | 2020-06-29 | 2022-01-05 | Zhejiang University of Science & Technology | Biochar-based fertilizer for improving foreshore saline-alkali soil, and preparation method and use thereof |
-
2017
- 2017-12-25 CN CN201711415683.7A patent/CN108083908A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109320355A (en) * | 2018-11-25 | 2019-02-12 | 江苏省农业科学院 | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application |
| CN109320355B (en) * | 2018-11-25 | 2021-07-30 | 江苏省农业科学院 | Bioactive conditioner for improving facility soil salinization and application thereof |
| EP3932896A1 (en) * | 2020-06-29 | 2022-01-05 | Zhejiang University of Science & Technology | Biochar-based fertilizer for improving foreshore saline-alkali soil, and preparation method and use thereof |
| CN113636895A (en) * | 2021-08-03 | 2021-11-12 | 沧州市农林科学院 | Microbial organic bacterial fertilizer, mixing and stirring device thereof and use method |
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Application publication date: 20180529 |