CN108079249A - A kind of Chinese medicine preparation with liver protection and preparation method thereof - Google Patents

A kind of Chinese medicine preparation with liver protection and preparation method thereof Download PDF

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Publication number
CN108079249A
CN108079249A CN201711461120.1A CN201711461120A CN108079249A CN 108079249 A CN108079249 A CN 108079249A CN 201711461120 A CN201711461120 A CN 201711461120A CN 108079249 A CN108079249 A CN 108079249A
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preparation
chinese medicine
medicine preparation
liver protection
parts
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薛强
周海平
李卫民
黄燕巧
连运河
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Chenguang Biotech Group Handan Co Ltd
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Chenguang Biotech Group Handan Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/233—Bupleurum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/36—Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/634—Forsythia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
    • A61K36/8888—Pinellia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8964—Anemarrhena
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

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  • Health & Medical Sciences (AREA)
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Abstract

The invention discloses a kind of Chinese medicine preparations with liver protection and preparation method thereof.The Chinese medicine preparation effective component of the present invention is made of radix bupleuri, Fructus Forsythiae, Radix Salviae Miltiorrhizae, turmeric, rhizoma anemarrhenae, radix pseudostellariae and rhizoma pinellinae praeparata, raw material crushes respectively, claim sample according to weight proportion, mixing, carried by alcohol extracting, water, be spray-dried and preparation and etc. preparation is made, the Chinese medicine preparation plays an important role of chemical damage treatment for improving liver function.The present invention is based on modern traditional Chinese medicine theory, parts of generic medicinal plants is used as raw material, it is extracted using modern scientific method, with advanced production technology, the detection means of modernization refines, raw material is simple and easy to get, simple and convenient extraction, product quality are stablized, and cost is relatively low, it is suitble to industrialized production, there is preferable Social benefit and economic benefit.

Description

A kind of Chinese medicine preparation with liver protection and preparation method thereof
Technical field
The invention belongs to pharmaceutical technology fields, and in particular to a kind of to have the function of the Chinese medicine preparation of liver protection and its preparation side Method.
Background technology
China is one of higher country of hepatopathy incidence, since life stress is excessive, life or working environment deteriorate, is led Hepatic injury number caused by reason contacts chemically poisonous substance (such as alcohol or other noxious materials) is also being on the increase, especially Excessive drinking and alcoholic are increasing or are engaged in the staff of industries such as chemicals synthesis, Chemical Manufacture chemically The number of hepatic injury gradually increases, and is also gradually increased by the acute and chronic hepatitis patients that chemical damage triggers.Hepatopathy is most prominent The symptom gone out is exactly lassitude and weak and do not feel like eating.Common sympton has distending pain, nausea, detests greasy, turgor or has jaundice after food, mouth Dry, stool is dry or half congealed, and urine is yellow or has low fever, dizzy tinnitus, and sallow complexion is simple and unadorned etc., has seriously affected the body and mind of people Health and family, the stabilization of society.
At present, there are the drug or Chinese medicine composition of various liver protectings in the market, be primarily present ineffective, preparation process The shortcomings of complexity is not suitable for industrialized production, expensive, increases the life stress of patient, therefore find and research and development are a kind of easily In prepare, the drug of evident in efficacy, inexpensive treatment hepatopathy is always the wish of researcher.
Radix bupleuri, it is pungent, bitter, it is slightly cold.Return liver, courage, lung channel.Evacuation is brought down a fever, soothing liver-qi stagnation, is risen and is lifted yang-energy.For cold, fever, Fevers and chills alternate, distending pain in the chest and hypochondrium, irregular menstruation, the prolapse of uterus, rectal prolapse（Chinese Pharmacopoeia Commission compiles《Pharmacopoeia of People's Republic of China》 Version one in 2015, page 281, China Medical Science Press）.Many researchs show that saikoside has hepatoprotective effect（It thanks Great, the Cai Baochang in east, pacifies the saikoside class chemical compositions such as benefit is strong and Advance on Pharmacological Activities [J] Nanjing University of Traditional Chinese Medicine is learned Report, 2007, (01):63-65.）.
Fructus Forsythiae, it is bitter, it is slightly cold.Return lung, the heart, small intestinl channel.It is clearing heat and detoxicating, dispersing swelling and dissipating binds, dispelling wind and heat from the body.For ulcer, scrofula, Acute mastitis, erysipelas, anemopyretic cold, warm disease is from the beginning of warm is joined the army, hyperpyrexia polydipsia, coma hair spot, puckery pain of heat gonorrhea（National Pharmacopeia committee member It can compile《Pharmacopoeia of People's Republic of China》Version one in 2015, page 171, China Medical Science Press）.Research shows Fructus Forsythiae Also there is anti-liver injury（Pharmaceutical research [J] contemporary Chinese application medicines of the Fructus Forsythiaes such as Zhang Wei, Zhang Hanming, Guo Meili It learns, 2000, (01):9-12.）.
Radix Salviae Miltiorrhizae, it is bitter, it is slightly cold.Converge to heart and liver channels.Promoting blood circulation, inducing meastruation to relieve menalgia, relieving restlessness that clears away heart-fire, cool blood to disappear carbuncle.For the obstruction of qi in the chest heart Bitterly, gastral cavity abdomen hypochondriac pain , lumps in the chest and abdomen, hot numbness pain, dysphoria and insomnia, irregular menstruation, dysmenorrhoea Amenorrhea, sore Ulcers swelling and pain（National Pharmacopeia is entrusted Member can compile《Pharmacopoeia of People's Republic of China》Version one in 2015, page 77, China Medical Science Press）.Some researches show that Radix Salviae Miltiorrhizae also has significant protective effect to hepatic injury（The Radix Salviae Miltiorrhizaes such as Yuan Yuan, Wu Qin, Shi Jing mountain and its main component hepatoprotective effect Progress [J] CHINA JOURNAL OF CHINESE MATERIA MEDICAs, 2015, (04):588-593.）.
Turmeric, pungent, bitter, temperature.Returns spleen, Liver Channel.Blood-breaking, it is inducing meastruation to relieve menalgia.For chest side of body shouting pain, chest is fought pained, dysmenorrhoea Amenorrhea , Disorder lumps in the abdomen, rheumatism shoulder arm pain, tumbling and swelling（Chinese Pharmacopoeia Commission compiles《Pharmacopoeia of People's Republic of China》Version in 2015 One, page 264, China Medical Science Press）.
Rhizoma anemarrhenae, it is bitter, sweet, it trembles with fear.Return lung, stomach, kidney channel.Clearing heat-fire, nourishing Yin and moistening dryness.For fever caused by exogenous pathogenic factors, hyperpyrexia polydipsia, lung Hot cough caused by dryness, osteopyrexia and fever, Heat Diabetes, dry constipation of intestines（Chinese Pharmacopoeia Commission compiles《Pharmacopoeia of People's Republic of China》2015 Version one, page 213, China Medical Science Press）.
Radix pseudostellariae, sweet, slight bitter are put down.Returns spleen, lung channel.It replenishes qi to invigorate the spleen, it is promoting production of body fluid and nourishing the lung.It is tired for spleen deficiency body, loss of appetite, Eak after being ill, deficiency of both vital energy and Yin, spontaneous perspiration is thirsty, dryness of the lung dry cough（Chinese Pharmacopoeia Commission compiles《Pharmacopoeia of People's Republic of China》2015 Year version one, page 68, China Medical Science Press）.
Rhizoma pinellinae praeparata, it is acrid in flavour and warm in nature.Returns spleen, stomach, lung channel.It is eliminating dampness and eliminating phlegm.For abundant expectoration cough and asthma, phlegm retention anti-dazzle nervous, the dizzy Halo of wind phlegm, phlegm Cryogenous headache（Chinese Pharmacopoeia Commission compiles《Pharmacopoeia of People's Republic of China》Version one in 2015, page 120, China Medical Science Publishing house）.
Above-mentioned 7 taste medicine use monarch combination principle, be used in combination there is no literature reported on, the present invention by experiment hair This existing 7 taste medical instrument has synergistic function, can prevent and treat chemical damage, has a hepatoprotective effect, and easily prepared, It is evident in efficacy, cost is relatively low, have preferable Social benefit and economic benefit.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of Chinese medicine preparations with liver protection and preparation method thereof；This Raw material is simple and easy to get in invention, and significant effect, preparation process is simple, is suitble to industrialized production, reduces cost and the life of patient Pressure, and liver function can be effectively improved, play an important role of to treat and protect to hepatic injury.
In order to solve the above technical problems, the present invention adopts the technical scheme that：It is a kind of that there is the Chinese medicine preparation of liver protection, The Chinese medicine preparation is made of effective component and/or pharmaceutically acceptable carrier, wherein the Chinese medicine preparation effective component by Following raw material composition：1-5 parts of radix bupleuri, 1-5 parts of Fructus Forsythiae, 1-5 parts of Radix Salviae Miltiorrhizae, 1-5 parts of turmeric, 1-5 parts of rhizoma anemarrhenae, 1-4 parts of radix pseudostellariae, method 1-4 parts of the tuber of pinellia.
The present invention also provides a kind of preparation method with the Chinese medicine preparation of liver protection, the preparation method include with Lower step：
（1）Pre-treatment：Raw material crushes respectively, claims sample, mixing according to weight proportion；
（2）Alcohol extracting：To step（1）The ethyl alcohol of 60-95% is added in after crushing, proportioning, mixing in raw material, flows back and carries at 60-90 DEG C It takes, filters, ethyl alcohol is recovered under reduced pressure, obtains thick paste；
（3）Water carries：To step（2）Pure water is added in material residue after filtering, is heated to 60-90 DEG C of extraction, filtering obtains filtrate；
（4）Spray drying：By step（2）In obtained thick paste and step（3）In obtain filtrate mixing, concentrate, spray drying, Obtain powder；
（5）Preparation：Step（4）In obtained powder add in pharmaceutically acceptable auxiliary material preparation be made.
Step of the present invention（1）Middle raw material was crushed to 10-100 mesh sieves respectively.
Step of the present invention（2）, to the ethyl alcohol for crushing, matching, adding in 4-14 times of volume after mixing in raw material, reflux carries When taking 0.5-3 small.
Step of the present invention（3）, the pure water that 4-14 times of volume is added in material residue, extraction are obtained by filtration after ethyl alcohol extraction When 0.5-3 is small.
Step of the present invention（5）, manufactured preparation is the dosage forms such as particle, piece or capsule.
Preparation of the present invention is oral formulations.
Preparation of the present invention is granule, tablet or capsule.
The present invention also provides a kind of above-mentioned application with the Chinese medicine preparation of liver protection, the particularly preparations for making Application in terms of standby treatment hepatic injury, improvement liver function drug.
The effect of prescription of the present invention is as follows：Radix bupleuri, Fructus Forsythiae are as monarch drug in a prescription in this side, and soothing liver-qi stagnation rises and lifts yang-energy, tool Play the role for the treatment of hepatic injury and liver protection；Radix Salviae Miltiorrhizae and turmeric can be inducing meastruation to relieve menalgia, monarch drug in a prescription liver protecting aided in, to symptoms such as distending pains There is mitigation, used in this side as ministerial drug；Rhizoma anemarrhenae and radix pseudostellariae are adjutant in this side, and QI invigorating righting has heat-clearing Purging intense heat, nourishing Yin and moistening dryness, the effect of moistening lung can effectively alleviate dizziness and tinnitus, the simple and unadorned symptom of sallow complexion；Rhizoma pinellinae praeparata can The therapeutic effect of monarch drug in a prescription radix bupleuri and Fructus Forsythiae, ministerial drug Radix Salviae Miltiorrhizae and turmeric, adjutant rhizoma anemarrhenae and radix pseudostellariae is cooperateed with, while can also be by all efficacy-enhancing ingredients The internal organs and channels and collaterals of required treatment are led, so make the effect for the treatment of hepatopathy more targeted, find that this 7 taste medical instrument has by experiment Synergistic function can prevent and treat chemical damage, have hepatoprotective effect.
It is using advantageous effect caused by above-mentioned technical proposal：1st, the present invention using monarch combination principle into Row allotment, all medicines share have synergistic function, can not only treat hepatopathy to a certain extent, and with liver protection shield The effect of liver；There is no incompatibility between each raw material, it can be long-term use of.2nd, turn of each effective component of chinese medicine in alcohol extracting part of the present invention Shifting rate is more than 90%, the recovery rate higher of active ingredient, and liver protecting is better；Water, which carries, such as to know water soluble ingredient The constituents extractions such as female polysaccharide come out, and it is hypoglycemic and other effects that research shows that anemaran has.3rd, the present invention is with modern traditional Chinese medicine Based on theory, parts of generic medicinal plants is used to be extracted for raw material using modern scientific method, with advanced production technology, modernization Detection means refine, raw material is simple and easy to get, simple and convenient extraction, product quality stablize, be suitble to industrialized production, cost It is relatively low, there is preferable Social benefit and economic benefit.
Specific embodiment
The present invention is elaborated further with reference to specific embodiment.
Embodiment 1
The preparation method of granule
1st, prescription
1 part of radix bupleuri, 1 part of Fructus Forsythiae, 1 part of Radix Salviae Miltiorrhizae, 1 part of turmeric, 1 part of rhizoma anemarrhenae, 1 part of radix pseudostellariae, 1 part of rhizoma pinellinae praeparata.
2nd, preparation method
（1）Pre-treatment：7 kinds of raw materials were crushed to 10 mesh sieves respectively, claim sample, mixing according to weight proportion；
（2）Alcohol extracting：To step（1）The ethyl alcohol of 60% purity of 4 times of volumes is added in after mixing in raw material, is heated to 60 DEG C, reflux carries Take 0.5 it is small when, filter to obtain extracting solution, the concentration that sampling detects each active ingredient calculates the rate of transform, and ethyl alcohol is recovered under reduced pressure, obtains thick Cream；The rate of transform of extracting solution is shown in Table 1：
In 1 embodiment 1 of table in alcohol extract active ingredient the rate of transform
（3）Water carries：To step（2）In the pure water of 4 times of volumes is added in the material residue that is obtained by filtration, it is small to be heated to 60 DEG C of extractions 0.5 When, filtering obtains filtrate；
（4）Spray drying：By step（2）In obtain thick paste and step（3）In obtain filtrate mixing, concentrate, spray drying, obtain Powder；
（5）Preparation：By step（4）In obtained powder add in cane sugar powder, be made particle, mixing, it is dry to get.
Embodiment 2
The preparation method of tablet
1st, prescription
5 parts of radix bupleuri, 5 parts of Fructus Forsythiae, 5 parts of Radix Salviae Miltiorrhizae, 5 parts of turmeric, 5 parts of rhizoma anemarrhenae, 4 parts of radix pseudostellariae, 4 parts of rhizoma pinellinae praeparata.
2nd, preparation method
（1）Pre-treatment：7 kinds of raw materials were crushed to 100 mesh sieves respectively, claim sample, mixing according to weight proportion；
（2）Alcohol extracting：To step（1）The ethyl alcohol of 95% purity of 14 times of volumes is added in after mixing in raw material, is heated to 90 DEG C, reflux Extract 3 it is small when, filter to obtain extracting solution, the concentration that sampling detects each active ingredient calculates the rate of transform, and ethyl alcohol is recovered under reduced pressure, obtains thick Cream；The rate of transform of extracting solution is shown in Table 2：
In 2 embodiment 2 of table in alcohol extract active ingredient the rate of transform
（3）Water carries：To step（2）In the pure water of 14 times of volumes is added in the material residue that is obtained by filtration, it is small to be heated to 90 DEG C of extractions 3 When, filtering obtains filtrate；
（4）Spray drying：By step（2）In obtain thick paste and step（3）In obtain filtrate mixing, concentrate, spray drying, obtain Powder；
（5）Preparation：By step（4）In obtained powder add in starch, magnesium stearate tabletting to get.
Embodiment 3
The preparation method of capsule
1st, prescription
3 parts of radix bupleuri, 3 parts of Fructus Forsythiae, 3 parts of Radix Salviae Miltiorrhizae, 3 parts of turmeric, 3 parts of rhizoma anemarrhenae, 2 parts of radix pseudostellariae, 1 part of rhizoma pinellinae praeparata.
2nd, preparation method
（1）Pre-treatment：7 kinds of raw materials were crushed to 50 mesh sieves respectively, claim sample, mixing according to weight proportion；
（2）Alcohol extracting：To step（1）The ethyl alcohol of 75% purity of 8 times of volumes is added in after mixing in raw material, is heated to 85 DEG C, reflux carries Take 1.5 it is small when, filter to obtain extracting solution, the concentration that sampling detects each active ingredient calculates the rate of transform, and ethyl alcohol is recovered under reduced pressure, obtains thick Cream；The rate of transform of extracting solution is shown in Table 3：
In 3 embodiment 3 of table in alcohol extract active ingredient the rate of transform
（3）Water carries：To step（2）In the pure water of 6 times of volumes is added in the material residue that is obtained by filtration, be heated to 85 DEG C of extractions 1 it is small when, Filtering, obtains filtrate；
（4）Spray drying：By step（2）In obtain thick paste and step（3）In obtain filtrate mixing, concentrate, spray drying, obtain Powder；
（5）Preparation：By step（4）In obtained powder add in starch, particle is made, it is dry, be packed into capsule to get.
Embodiment 4
The preparation method of tablet
1st, prescription
1 part of radix bupleuri, 1 part of Fructus Forsythiae, 1 part of Radix Salviae Miltiorrhizae, 1 part of turmeric, 1 part of rhizoma anemarrhenae, 4 parts of radix pseudostellariae, 3 parts of rhizoma pinellinae praeparata.
2nd, preparation method
（1）Pre-treatment：7 kinds of raw materials were crushed to 30 mesh sieves respectively, claim sample, mixing according to weight proportion；
（2）Alcohol extracting：To step（1）The ethyl alcohol of 95% purity of 4 times of volumes is added in after mixing in raw material, is heated to 60 DEG C, reflux carries Take 3 it is small when, filter to obtain extracting solution, the concentration that sampling detects each active ingredient calculates the rate of transform, and ethyl alcohol is recovered under reduced pressure, obtains thick paste； The rate of transform of extracting solution is shown in Table 4：
In 4 embodiment 4 of table in alcohol extract active ingredient the rate of transform
（3）Water carries：To step（2）In the pure water of 14 times of volumes is added in the material residue that is obtained by filtration, it is small to be heated to 60 DEG C of extractions 3 When, filtering obtains filtrate；
（4）Spray drying：By step（2）In obtain thick paste and step（3）In obtain filtrate mixing, concentrate, spray drying, obtain Powder；
（5）Preparation：By step（4）In obtained powder add in starch, magnesium stearate tabletting to get.
Embodiment 5
The present embodiment is intended to prove the liver protective effect of Chinese medicine preparation of the present invention.
Experiment content is as follows：
1st, animal：50 male rats（SD kinds）.
2nd, drug and reagent：Dispenser group is that spray drying in the embodiment of the present invention 1 obtains powder with drug；Carbon tetrachloride （CCl4）For commercially available gained.
3rd, grouping and drug dose：Above-mentioned 50 rats are randomly divided into 5 groups, rat every group 10 is respectively：
（1）Blank control group：Normal raising；
（2）Model group：Using CCl4Duplication is normally raised after obtaining liver injury model；
（3）Low dosage dispenser group：First apply CCl4Duplication obtains liver injury model, afterwards by gavage after drug powder water dissolution Administration, dosage 140mg/kg, continuous 30 days；
（4）Middle dosage dispenser group：First apply CCl4Duplication obtains liver injury model, afterwards by gavage after drug powder water dissolution Administration, dosage 280mg/kg, continuous 30 days；
（5）High dose dispenser group：First apply CCl4Duplication obtains liver injury model, afterwards by gavage after drug powder water dissolution Administration, dosage 560mg/kg, continuous 30 days.
4th, measure and result
（1）When testing the fasting 16 overnight of each group animal is small in the 30th day, model group and basic, normal, high dosage dispenser group once fill Stomach gives CCl4, to vegetable oil, basic, normal, high dosage dispenser group continues to give drug to experiment to be terminated blank control group（With CCl4 When gavage interval 4 is small）.Animal is put to death after when 24 is small, takes blood system from serum, the serum glutamic oxalacetic transaminase of measure each group rat (AST) and the content of glutamic-pyruvic transaminase (ALT) 5, be the results are shown in Table：
The measurement result of 5 rat blood serum AST and ALT activity of table
Compared with model group, basic, normal, high dosage dispenser group can significantly reduce the level of activity of AST and ALT（* P ＜ 0.01, * P ＜ 0.05）, wherein middle dosage and the effect of high dose dispenser group be most prominent.
（2）Rat liver lobus sinister is taken to be fixed with 10% formalin, cross section materials, routine pathology are done from left lobe of liver middle part Film-making carries out microscopy, observes pathological change, and hepatic tissue pathology damage criterion is shown in Table 6：
6 hepatic tissue pathology of table damages criterion
5th, 7 be the results are shown in Table：
The judging result of 7 liver tissues of rats pathological change of table
Compared with blank control group, hepatic injury degree substantially increases weight model group（* P ＜ 0.01）, represent modeling success.
Compared with model group, the hepatic tissue pathology variation of basic, normal, high dosage dispenser group rat has significant improvement（**P ＜ 0.01, * P ＜ 0.05）, what wherein curative effect was the most prominent is middle and high dosage dispenser group, and it is significantly anti-to illustrate that drug of the present invention has The effect of hepatic injury.
6th, conclusion：Drug of the present invention, which has Liver Damage in Rats, treats and protects liver effect.
The above embodiments are only used to illustrate and not limit the technical solutions of the present invention, although with reference to above-described embodiment to this hair It is bright to be described in detail, it will be understood by those of ordinary skill in the art that：Still can the present invention be modified or be waited With replacing, without departing from the spirit or scope of the invention, or any substitutions, the power in the present invention should all be covered Among sharp claimed range.

Claims (9)

1. a kind of have the function of the Chinese medicine preparation of liver protection, which is characterized in that the Chinese medicine preparation is by effective component and/or pharmaceutically Acceptable carrier composition, wherein the Chinese medicine preparation effective component is made of following raw material：1-5 parts of radix bupleuri, 1-5 parts of Fructus Forsythiae, 1-5 parts of Radix Salviae Miltiorrhizae, 1-5 parts of turmeric, 1-5 parts of rhizoma anemarrhenae, 1-4 parts of radix pseudostellariae, 1-4 parts of rhizoma pinellinae praeparata.
2. based on a kind of preparation method with the Chinese medicine preparation of liver protection described in claim 1, which is characterized in that described Preparation method comprises the following steps：
（1）Pre-treatment：Raw material crushes respectively, claims sample, mixing according to weight proportion；
（2）Alcohol extracting：To step（1）The ethyl alcohol of 60-95% is added in after crushing, proportioning, mixing in raw material, flows back and carries at 60-90 DEG C It takes, filters, ethyl alcohol is recovered under reduced pressure, obtains thick paste；
（3）Water carries：To step（2）Pure water is added in material residue after filtering, is heated to 60-90 DEG C of extraction, filtering obtains filtrate；
（4）Spray drying：By step（2）In obtained thick paste and step（3）In obtain filtrate mixing, concentrate, spray drying, Obtain powder；
（5）Preparation：Step（4）In obtained powder add in pharmaceutically acceptable auxiliary material preparation be made.
3. a kind of preparation method with the Chinese medicine preparation of liver protection according to claim 2, which is characterized in that described Step（1）Middle raw material was crushed to 10-100 mesh sieves respectively.
4. a kind of preparation method with the Chinese medicine preparation of liver protection according to claim 2, which is characterized in that described Step（2）, the ethyl alcohol of 4-14 times of volume of addition into raw material after crushing, proportioning, mixing, when refluxing extraction 0.5-3 is small.
5. a kind of preparation method with the Chinese medicine preparation of liver protection according to claim 2, which is characterized in that described Step（3）, the pure water of 4-14 times of volume is added in into the material residue that is obtained by filtration after ethyl alcohol extraction, when extraction 0.5-3 is small.
6. a kind of preparation method with the Chinese medicine preparation of liver protection according to claim 2, which is characterized in that described Step（5）, manufactured preparation is the dosage forms such as particle, piece or capsule.
7. a kind of preparation method with the Chinese medicine preparation of liver protection according to claim 6, which is characterized in that described Preparation is oral formulations.
8. a kind of preparation method with the Chinese medicine preparation of liver protection according to claim 7, which is characterized in that described Preparation is granule, tablet or capsule.
9. a kind of application with the Chinese medicine preparation of liver protection described in based on claim 2-8 any one, feature exist In the preparation is used to prepare the application in terms for the treatment of hepatic injury, improvement liver function drug.
CN201711461120.1A 2017-12-28 2017-12-28 A kind of Chinese medicine preparation with liver protection and preparation method thereof Pending CN108079249A (en)

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Citations (4)

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Publication number Priority date Publication date Assignee Title
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CN103372174A (en) * 2012-04-27 2013-10-30 王宇红 Compound traditional Chinese medicine for treating depression
CN104173426A (en) * 2014-08-21 2014-12-03 常熟雷允上制药有限公司 Traditional Chinese medicine composition with liver-protecting function, as well as preparation method and application thereof
CN104707101A (en) * 2015-02-26 2015-06-17 天津大学 Liver cancer and lung cancer resistant traditional Chinese medicine composition and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101607063A (en) * 2008-06-20 2009-12-23 天科仁祥技术（北京）有限责任公司 Drug regimen of a kind of fat-reducing liver-protecting and preparation method thereof
CN103372174A (en) * 2012-04-27 2013-10-30 王宇红 Compound traditional Chinese medicine for treating depression
CN104173426A (en) * 2014-08-21 2014-12-03 常熟雷允上制药有限公司 Traditional Chinese medicine composition with liver-protecting function, as well as preparation method and application thereof
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