CN108077941A - A kind of algae peptide combinations and preparation method and application - Google Patents
A kind of algae peptide combinations and preparation method and application Download PDFInfo
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- CN108077941A CN108077941A CN201711399054.XA CN201711399054A CN108077941A CN 108077941 A CN108077941 A CN 108077941A CN 201711399054 A CN201711399054 A CN 201711399054A CN 108077941 A CN108077941 A CN 108077941A
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- algae
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- freshwater mussel
- enzymolysis
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/19—Dairy proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses algae peptide combinations, it prepares raw material and is formed by mixing algae, egg mix white powder and freshwater mussel meat, the mixing algae is made of chlorella pyrenoidosa, Euglena and salt algae, the egg mix white powder by pea protein, dragon protein and first milk basic protein form.The preparation method of the algae peptide combinations comprises the following steps：1) pre-treatment of algae is mixed；2) it is levigate；3) pre-treatment of freshwater mussel meat；4) ferment；5) fat enzymolysis；6) tryptose digests；7) basic protein digests；8) bitter off-flavors and 9) drying are taken off.The algae peptide combinations that the present invention obtains, with preferable nutrition-allocated proportion, the distribution of balanced amino acid content and preferable flavor and condition.
Description
Technical field
The present invention relates to a kind of healthy food more particularly to a kind of algae peptide combinations and preparation method thereof.
Background technology
Protein peptides are that protein is processed further to the product of processing using biological enzymolysis technology.Protein peptides are because of strand phase
To smaller, it is easier to digest and assimilate, for system function crowd on the weak side be extraordinary mode for digesting and assimilating.Albumen
Peptide has preferable bioactivity and stability, there is considerable application prospect in skin care item and field of health care products.
The processing of protein peptides and zymolysis technique are relatively difficult to control, how on the basis of its original nutritive peculiarity is not influenced
The conversion ratio of product is improved, and to ensure the presence of its physiological activator, it is necessary to conscientiously be studied and inquired into.The opposing party
Face, protein peptides are also easy to produce bitter off-flavors in hydrolytic process, this into its health products especially healthy food application aspect
Obstacle.
Seaweed peptide is that its a kind of chemical structural formula mainly has dipeptides, cyclic peptide and lipopeptid, these peptides have anti-
Tumour, lowering blood pressure and blood fat, promote neural cellular differentiation, anti-oxidant, antibacterial and the bioactivity such as antiviral at anticoagulation.By doing
Seaweed process to obtain algae peptide and be typically passed through broken wall and enzymolysis.
Anti-oxidation peptide can effectively remove the active oxygen radical of internal surplus, protect cell and mitochondria normal configuration and
Function prevents the generation of lipid peroxidation, mitigates body and is damaged, so as to avoid the human diseases caused by oxidative damage
Such as cancer, aging, chronic traumatic lung disease and the generation of artery sclerosis disease.
Research shows that the anti-oxidation peptide of marine source has a variety of physiological activity, as can inhibiting long wave ultraviolet to Hela
The oxidative damage of cell, the oxidative damage for mitigating thymocyte, the apoptosis for inhibiting thymocyte etc..
The content of the invention
For overcome the deficiencies in the prior art, it is an object of the invention to provide a kind of mouthfeel it is good, rich in the peptide easily absorbed,
Aliphatic acid, be conducive to human body minerals algae peptide combinations.
The second object of the present invention is the preparation method for providing the algae peptide combinations.
The third object of the present invention is the application for providing the algae peptide combinations.
An object of the present invention adopts the following technical scheme that realization：
A kind of algae peptide combinations prepare raw material and are formed by mixing algae, egg mix white powder and freshwater mussel meat, the mixing algae
Be made of chlorella pyrenoidosa, Euglena and salt algae, the egg mix white powder by pea protein, dragon protein and first milk basic protein
Composition.
Further, raw material is prepared by mixing algae, egg mix white powder and the freshwater mussel meat in terms of dry weight by (15-20)：
(2.5-3.5)：1 weight ratio composition.
Further, it is described to mix algae by chlorella pyrenoidosa, Euglena and the salt algae in terms of dry weight by (5-10)：(5-
10)：2 weight ratio composition.
Further, the egg mix white powder by pea protein, dragon protein and first milk basic protein press (6-8)：(2-
3)：1 weight ratio composition.
The second object of the present invention adopts the following technical scheme that realization：
The preparation method of above-mentioned algae peptide combinations, comprises the following steps：
1) pre-treatment of algae is mixed：The clean, chlorella pyrenoidosa without sand is chosen, is placed in autoclave, is passed through
Saturated vapor is heated to 90-95 DEG C, maintains 5-10min, is forced into 1.2-1.4MPa, instantaneously reduces to normal pressure, obtain broken wall albumen
Core chlorella；
2) it is levigate：Euglena and salt algae are added in into the broken wall chlorella pyrenoidosa that step 1) obtains, adds 0.5-5 times of weight
Water in colloid mill was ground to the sieving rate of 200 mesh sieves up to more than 95%, and obtained algae slurries repeatedly；
3) pre-treatment of freshwater mussel meat：Freshwater mussel meat is taken to be beaten, is freezed, is placed in autoclave, is passed through saturated vapor and is heated to 90-
95 DEG C, maintain 5-10min, after be forced into 1.2-1.4MPa, instantaneously reduce to normal pressure, add in the water of 0.5-5 times of weight, homogenate obtains
To freshwater mussel meat liquid；
4) ferment：Bacillus subtilis is accessed into the freshwater mussel meat liquid that step 3) obtains, ferment 10-20min, boils inactivation,
Obtain freshwater mussel meat fermentation liquid；
5) fat enzymolysis：It is mixed to the algae slurries that step 1) obtains with the freshwater mussel meat fermentation liquid that step 3) obtains, adds in fat
Enzyme, ultrasonic wave added enzymolysis, when standing 1-2 is small, boils inactivation, after being down to 35-40 DEG C, obtains the first enzymolysis liquid；
6) tryptose digests：Egg mix white powder is added in the first enzymolysis liquid obtained to step 5), adds in trypsase, is adjusted
PH to 6.0-6.5 is saved, ultrasonic wave added enzymolysis 10-30min when standing 1-2 is small, boils inactivation, obtains the second enzymolysis liquid；
7) basic protein digests：Alkali protease is added in the second enzymolysis liquid obtained to step 5), adjusts pH to 7.0-
7.5, ultrasonic wave added enzymolysis 10-30min when standing 1-2 is small, boils inactivation, obtain the second enzymolysis liquid；
8) bitter off-flavors are taken off：The pH of enzymolysis liquid is adjusted as 7.0-7.5, adds in the activated carbon of 1-3%, centrifugation takes supernatant；
9) it is dry：Thick liquid is concentrated under reduced pressure into, is spray-dried, obtains algae peptide combinations.
Further, in step 3), the addition of the bacillus subtilis is the 0.5-1% of freshwater mussel jerky weight, fermentation temperature
It spends for 42-45 DEG C.
Further, in step 5), the enzyme concentration of lipase is the 0.1-0.5% of raw material gross weight, and ultrasonic power is
200-300W。
Further, in step 6), the enzyme concentration of trypsase is the 2-3% of raw material gross weight, and ultrasonic power is
200-300W。
Further, in step 7), the enzyme concentration of alkali protease is the 2-3% of raw material gross weight.
Further, in step 5) and/or step 6) and/or step 7), the concrete operations of ultrasonic wave added enzymolysis are：Ultrasound
After 10-30s, suspend 1-3min, then ultrasonic wave added 20-30min.
The third object of the present invention adopts the following technical scheme that realization：
Application of the above-mentioned algae peptide combinations in healthy food.
Compared with prior art, the beneficial effects of the present invention are：
1) algae peptide combinations provided by the invention, in terms of the nutritional ingredient for, amino acid content distribution and the combination
It is adapted needed for peptide, aliphatic acid and content of mineral substances and health in object, is beneficial to maintain health vigor, reduce certainly
By the infringement of the oxidation sources such as base；
2) algae peptide combinations provided by the invention, in terms of the physicochemical property for, in the algae peptide combinations preparation process, energy
It effectively removes the harmful substances such as the heavy metal ion that algae is adsorbed in growing environment, retain the mineral elements such as calcium；The algae peptide group
Object is closed after broken wall, fermentation and enzymolysis, in vivo heavy metal can be removed after edible, harmful substance in alimentary canal；
3) algae peptide combinations provided by the invention, in terms of the flavor and taste for, which overcome hydrobiontic alga and bivalves
The unhappiness aroma and flavor such as bad smell, bitter taste in process, while overcome stiff indigestibility protein indissoluble in freshwater mussel
The problem of going out；The algae peptide combinations have preferable flavor, mouthfeel and condition.
Specific embodiment
In the following, with reference to specific embodiment, the present invention is described further, it is necessary to which explanation is, what is do not collided
Under the premise of, new embodiment can be formed between various embodiments described below or between each technical characteristic in any combination.
The present invention provides a kind of algae peptide combinations, prepares raw material and is formed by mixing algae, egg mix white powder and freshwater mussel meat, institute
State mixing algae be made of chlorella pyrenoidosa, Euglena and salt algae, the egg mix white powder by pea protein, dragon protein and just
Milk basic protein forms.
Algae peptide combinations provided by the invention are that algae, albumen powder and freshwater mussel meat are combination, are by algae protein, plant egg
White and animal protein is combined, to meet the peptide demand of different levels；The mixing algae be by the Euglena of acellular wall, salt algae with
The chlorella pyrenoidosa for having cell membrane is engaged, and during working process, broken wall process is simple, and the composition processed contains
Suitable cellulose components, can effectively remove in vivo toxin and heavy metal ion, at the same to the burden in gastrointestinal disturbances road compared with
It is small.
For the health products using protein as nutritional ingredient feature, multiclass nutritional ingredient is mainly included to the evaluation of its quality
Equilibrium degree, the balanced degree of amino acid and the distribution of peptide molecular weight.Algae peptide combinations provided by the invention, by by animal
Source nutritional ingredient carries out complementation with algae source nutritional ingredient, so that said composition has preferable aliphatic acid, minerals, peptide content ratio
Example and with human body must amino acid match amino acid content distribution.Method provided by the invention, the peptide digested,
Molecular weight is mainly distributed as 100-500Da, has preferable antioxidation activity and active anticancer.
Algae peptide combinations provided by the invention, the distribution of the species of the amino acid obtained based on protein digestion, pass through reason
The collocation mode in preferable peptide source, i.e. algae source, animal sources and plant source phase have been obtained by analysis and Test Summary repeatedly
Mutually cooperation, to form suitable aminoacid ingredient content distribution, preferable physiological activity, with meeting human nutrition demand collaboration model
Improve immunity：
1) for algae source, the active peptide with physiological activity mainly includes dipeptides, cyclic peptide and lipopeptid；In algae peptide
In enzymolysis product, human body must amino acid content it is higher include alanine, phenylalanine and leucine, and methionine, color
Propylhomoserin amount deficiency；
2) for freshwater mussel meat, in addition to containing abundant minerals such as calcium, protein content is high, and fat content passes through less
Good aliphatic acid can be made in hydrolysis, and such as DHA and ARA, help improves the immunity of the human body and is beneficial to cerebral nervous system；
3) it is effective supplement for algae source peptide and freshwater mussel meat source peptide for compound protein powder；Using the form of albumen powder,
Anaphylactogen can be effectively reduced and reduce cholesterol so that the audient of the algae peptide combinations is wider, more meets healthy green
The demand of diet；
4) leucine necessary to human body, phenylalanine, different is rich in from propylhomoserin acid balance angle, three kinds of algae
Leucine, and unfortunately, tryptophan and methionine contents are on the low side；And in freshwater mussel meat, phenylalanine, isoleucine, tryptophan
It is worth relatively low, but methionine contents are relatively high；Pea protein tryptophane is higher, be freshwater mussel meat and algae it is good mutually
It mends；In ground dragon protein, rich in valine, leucine, phenylalanine and lysine, three kinds of ingredients are mutual with preferable amino acid
It mends, synergistic effect.
In the present invention, the raw material in algae source also further by chlorella pyrenoidosa, Euglena and the salt algae in terms of dry weight by
(5-10)：(5-10)：2 weight ratio composition.The cooperation of three kinds of algaes promotes health to concertedness：
1) for the raw material in algae source, the presence of cell membrane hinders the release that protein treats active ingredient, also digests
System causes certain pressure；It is mutually tied with salt algae with the Euglena of acellular wall using the chlorella for having cell membrane in the present invention
It closes, improves the efficiency of Separation of Proteins；The cell membrane of chlorella pyrenoidosa, which is divided into, to be helped to remove the in vivo cholesterol of people, poison
Element and heavy metal；
2) polyoses content is high especially in chlorella and Euglena in three kinds of algae, and preparation method of the invention can be effectively
Preserve polysaccharides, for improve human immunological competence and it is antitumor have larger facilitation；
3) salt algae is rich in grease and beta carotene, and Euglena is rich in Euglena polysaccharide.
In the present invention, as the complementary element in algae source and freshwater mussel meat source, egg mix white powder by pea protein, dragon protein and
First milk basic protein presses (6-8)：(2-3)：1 weight ratio composition.Egg mix white powder contributes to the flat of amino acid as preceding described
It weighs, form the distribution of comparatively ideal peptide molecular weight, meanwhile, also significantly reduce the content of anaphylactogen and cholesterol.
Present invention simultaneously provides a kind of preparation methods of above-mentioned algae peptide combinations, comprise the following steps：
1) pre-treatment of algae is mixed：The clean, chlorella pyrenoidosa without sand is chosen, is placed in autoclave, is passed through
Saturated vapor is heated to 90-95 DEG C, maintains 5-10min, is forced into 1.2-1.4MPa, instantaneously reduces to normal pressure, obtain broken wall albumen
Core chlorella；
2) it is levigate：Euglena and salt algae are added in into the broken wall chlorella pyrenoidosa that step 1) obtains, adds 0.5-5 times of weight
Water in colloid mill was ground to the sieving rate of 200 mesh sieves up to more than 95%, and obtained algae slurries repeatedly；
3) pre-treatment of freshwater mussel meat：Freshwater mussel meat is taken to be beaten, is freezed, is placed in autoclave, is passed through saturated vapor and is heated to 90-
95 DEG C, maintain 5-10min, after be forced into 1.2-1.4MPa, instantaneously reduce to normal pressure, add in the water of 0.5-5 times of weight, homogenate obtains
To freshwater mussel meat liquid；
4) ferment：Bacillus subtilis is accessed into the freshwater mussel meat liquid that step 3) obtains, ferment 10-20min, boils inactivation,
Obtain freshwater mussel meat fermentation liquid；
5) fat enzymolysis：It is mixed to the algae slurries that step 1) obtains with the freshwater mussel meat fermentation liquid that step 3) obtains, adds in fat
Enzyme, ultrasonic wave added enzymolysis, when standing 1-2 is small, boils inactivation, after being down to 35-40 DEG C, obtains the first enzymolysis liquid；
6) tryptose digests：Egg mix white powder is added in the first enzymolysis liquid obtained to step 5), adds in trypsase, is adjusted
PH to 6.0-6.5 is saved, ultrasonic wave added enzymolysis 10-30min when standing 1-2 is small, boils inactivation, obtains the second enzymolysis liquid；
7) basic protein digests：In the second enzymolysis liquid obtained to step 5) add in basic protein enzymolysis, adjust pH to
7.0-7.5, ultrasonic wave added enzymolysis 10-30min, when standing 1-2 is small, boils inactivation, obtains the second enzymolysis liquid；
8) bitter off-flavors are taken off：The pH of enzymolysis liquid is adjusted as 7.0-7.5, adds in the activated carbon of 1-3%, centrifugation takes supernatant；
9) it is dry：Thick liquid is concentrated under reduced pressure into, is spray-dried, obtains algae peptide combinations.
Evaluate the quality of health products characterized by protein equilibrium degree of the index except above-mentioned multiclass nutritional ingredient,
Outside the molecular weight distribution of peptide, flavor taste and stability are further included.Method provided by the invention passes through mechanical means, thermodynamics
The mode that means and biochemical apparatus are combined so that peptide of the molecular weight distribution of final peptide in 100-500Da accounts for protein content
70-80%, the generation that effectively reduces bitter off-flavors, product be unlikely to deteriorate：
1) mode of steam blasting is employed, steam is made to fill raw cell, rapid aging protein, instantaneous controlled pressure drop, from
The outside explosion of cell interior, so as to effectively promote the release of protein, fat, minerals；
2) by the way of homogenate and colloidal grinding, the release of algae nutritional ingredient is promoted；
3) by the way of fermentation, the releasability of freshwater mussel meat nutritional ingredient and property easy to digest is improved, decreases freshwater mussel meat
Bitter off-flavors；
4) by the way of activated carbon adsorption, the heavy metal ion in bitter off-flavors ingredient and algae source is effectively removed, separately
On the one hand decolourize, make the quality higher of the algae peptide.
Embodiment 1：
A kind of algae peptide combinations prepare raw material by mixing algae, egg mix white powder and the freshwater mussel meat in terms of dry weight by 18:3:
1 weight ratio composition, the mixing algae is by chlorella pyrenoidosa, Euglena and salt algae by 7.5:7.5:2 compositions；Egg mix white powder
By pea protein, dragon protein and first milk basic protein press 7：2.5：1 composition；Its preparation method comprises the following steps：
1) pre-treatment of algae is mixed：The clean, chlorella pyrenoidosa without sand is chosen, is placed in autoclave, is passed through
Saturated vapor is heated to 92 DEG C, maintains 8min, is forced into 1.3MPa, normal pressure is reduced in 1s, obtain broken wall chlorella pyrenoidosa；
2) it is levigate：Euglena and salt algae are added in into the broken wall chlorella pyrenoidosa that step 1) obtains, adds the water of 3 times of weight,
The sieving rate of 200 mesh sieves was ground to repeatedly in colloid mill up to more than 95%, obtains algae slurries；
3) pre-treatment of freshwater mussel meat：Freshwater mussel meat is taken to be beaten, is freezed, is placed in autoclave, is passed through saturated vapor and is heated to 92
DEG C, 8min is maintained, is forced into 1.3MPa, instantaneously reduces to normal pressure, adds in the water of 2 times of weight, homogenate obtains freshwater mussel meat liquid；
4) ferment：The bacillus subtilis that freshwater mussel jerky weighs 0.8%, 44 DEG C of fermentations are accessed into the freshwater mussel meat liquid that step 3) obtains
15min boils inactivation, obtains freshwater mussel meat fermentation liquid；
5) fat enzymolysis：It is mixed to the algae slurries that step 1) obtains with the freshwater mussel meat fermentation liquid that step 3) obtains, adds in freshwater mussel meat
0.25% lipase of dry weight and algae total weight stops 2min ultrasound 25min again after 250W ultrasounds 10s, when standing 1.5 is small,
Inactivation is boiled, after being down to 38 DEG C, obtains the first enzymolysis liquid；
6) tryptose digests：Egg mix white powder is added in the first enzymolysis liquid obtained to step 5), adds in freshwater mussel jerky weight, algae
With the trypsase of egg mix white powder total weight 2.5%, stopping 2min is ultrasonic again after adjusting pH to 6.3,250W ultrasounds 10s
25min when standing 1.5 is small, boils inactivation, obtains the second enzymolysis liquid；
7) basic protein digests：Alkali protease is added in the second enzymolysis liquid obtained to step 5), adjusts pH to 7.3,
Stop 2min ultrasound 25min again after 250W ultrasounds 10s, when standing 1.5 is small, boils inactivation, obtain the second enzymolysis liquid；
8) bitter off-flavors are taken off：The pH for adjusting enzymolysis liquid is 7.3, adds in freshwater mussel jerky weight, algae and egg mix white powder total weight 2%
Activated carbon, centrifugation, takes supernatant；
9) it is dry：Thick liquid is concentrated under reduced pressure into, is spray-dried, obtains algae peptide combinations.
Embodiment 2：
A kind of algae peptide combinations prepare raw material by mixing algae, egg mix white powder and the freshwater mussel meat in terms of dry weight by 15:
3.5:1 weight ratio composition, the mixing algae is by chlorella pyrenoidosa, Euglena and salt algae by 5:10:2 compositions；Mixed protein
Powder by pea protein, dragon protein and first milk basic protein press 6：3：1 composition；Its preparation method comprises the following steps：
1) pre-treatment of algae is mixed：The clean, chlorella pyrenoidosa without sand is chosen, is placed in autoclave, is passed through
Saturated vapor is heated to 95 DEG C, maintains 10min, is forced into 1.4MPa, normal pressure is reduced in 1s, obtain broken wall chlorella pyrenoidosa；
2) it is levigate：Euglena and salt algae are added in into the broken wall chlorella pyrenoidosa that step 1) obtains, adds the water of 2 times of weight,
The sieving rate of 200 mesh sieves was ground to repeatedly in colloid mill up to more than 95%, obtains algae slurries；
3) pre-treatment of freshwater mussel meat：Freshwater mussel meat is taken to be beaten, is freezed, is placed in autoclave, is passed through saturated vapor and is heated to 95
DEG C, 10min is maintained, is forced into 1.4MPa, instantaneously reduces to normal pressure, adds in the water of 3 times of weight, homogenate obtains freshwater mussel meat liquid；
4) ferment：The bacillus subtilis that freshwater mussel jerky weighs 1%, 42 DEG C of fermentations are accessed into the freshwater mussel meat liquid that step 3) obtains
15min boils inactivation, obtains freshwater mussel meat fermentation liquid；
5) fat enzymolysis：It is mixed to the algae slurries that step 1) obtains with the freshwater mussel meat fermentation liquid that step 3) obtains, adds in freshwater mussel meat
0.5% lipase of dry weight and algae total weight stops 3min ultrasound 30min again after 250W ultrasounds 30s, when standing 1.5 is small, boils
Boiling inactivation, after being down to 38 DEG C, obtains the first enzymolysis liquid；
6) tryptose digests：Egg mix white powder is added in the first enzymolysis liquid obtained to step 5), adds in freshwater mussel jerky weight, algae
With the trypsase of egg mix white powder total weight 2.5%, stopping 3min is ultrasonic again after adjusting pH to 6.3,250W ultrasounds 30s
30min when standing 1.5 is small, boils inactivation, obtains the second enzymolysis liquid；
7) basic protein digests：Alkali protease is added in the second enzymolysis liquid obtained to step 5), adjusts pH to 7.5,
Stop 3min ultrasound 30min again after 250W ultrasounds 30s, when standing 1.5 is small, boils inactivation, obtain the second enzymolysis liquid；
8) bitter off-flavors are taken off：The pH for adjusting enzymolysis liquid is 7.5, adds in freshwater mussel jerky weight, algae and egg mix white powder total weight 1%
Activated carbon, centrifugation, takes supernatant；
9) it is dry：Thick liquid is concentrated under reduced pressure into, is spray-dried, obtains algae peptide combinations.
Embodiment 3：
A kind of algae peptide combinations prepare raw material by mixing algae, egg mix white powder and the freshwater mussel meat in terms of dry weight by 15:
3.5:1 weight ratio composition, the mixing algae is by chlorella pyrenoidosa, Euglena and salt algae by 10:5:2 compositions；Mixed protein
Powder by pea protein, dragon protein and first milk basic protein press 8：2：1 composition；Its preparation method comprises the following steps：
1) pre-treatment of algae is mixed：The clean, chlorella pyrenoidosa without sand is chosen, is placed in autoclave, is passed through
Saturated vapor is heated to 90 DEG C, maintains 5min, is forced into 1.2MPa, normal pressure is reduced in 1s, obtain broken wall chlorella pyrenoidosa；
2) it is levigate：Euglena and salt algae are added in into the broken wall chlorella pyrenoidosa that step 1) obtains, adds the water of 2 times of weight,
200 sieve rates were ground to repeatedly in colloid mill up to more than 95%, obtain algae slurries；
3) pre-treatment of freshwater mussel meat：Freshwater mussel meat is taken to be beaten, is freezed, is placed in autoclave, is passed through saturated vapor and is heated to 90
DEG C, 5min is maintained, is forced into 1.2MPa, instantaneously reduces to normal pressure, adds in the water of 3 times of weight, homogenate obtains freshwater mussel meat liquid；
4) ferment：The bacillus subtilis that freshwater mussel jerky weighs 0.5%, 45 DEG C of fermentations are added in into the freshwater mussel meat liquid that step 3) obtains
20min boils inactivation, obtains freshwater mussel meat fermentation liquid；
5) fat enzymolysis：It is mixed to the algae slurries that step 1) obtains with the freshwater mussel meat fermentation liquid that step 3) obtains, adds in freshwater mussel meat
0.1% lipase of dry weight and algae total weight stops 3min ultrasound 30min again after 250W ultrasounds 30s, when standing 1.5 is small, boils
Boiling inactivation, after being down to 38 DEG C, obtains the first enzymolysis liquid；
6) tryptose digests：Egg mix white powder is added in the first enzymolysis liquid obtained to step 5), adds in freshwater mussel jerky weight, algae
With the trypsase of egg mix white powder total weight 2.5%, stopping 3min is ultrasonic again after adjusting pH to 6.3,250W ultrasounds 30s
30min when standing 1.5 is small, boils inactivation, obtains the second enzymolysis liquid；
7) basic protein digests：Alkali protease is added in the second enzymolysis liquid obtained to step 5), adjusts pH to 7.5,
Stop 3min ultrasound 30min again after 250W ultrasounds 30s, when standing 1.5 is small, boils inactivation, obtain the second enzymolysis liquid；
8) bitter off-flavors are taken off：The pH for adjusting enzymolysis liquid is 7.5, adds in freshwater mussel jerky weight, algae and egg mix white powder total weight 3%
Activated carbon, centrifugation, takes supernatant；
9) it is dry：Thick liquid is concentrated under reduced pressure into, is spray-dried, obtains algae peptide combinations.
Comparative example 1：
Comparative example 1 as different from Example 1, is fermented without step 4).
Comparative example 2：
Comparative example 2 takes off bitter off-flavors as different from Example 1, without step 8).
The algae peptide combinations that embodiment 1-3 is obtained can be compounded further with five flours, and the guarantors such as meal replacing food are made
Strong product.
Function and effect assessment
1. flavor evaluation
55 volunteers are convened at this center, are randomly divided into 5 groups, every group of 11 people, everyone distributes embodiment 1-3 and comparative example 1-
2 obtained algae peptide combinations, are tasted by random order and fishy smell, bitter taste, tart flavour and delicate flavour are evaluated, by 1-10
Divide from gently to dense carry out subjective assessment, the results are shown in table below：
1 flavor evaluation table of table
| Fishy smell | Bitter taste | Tart flavour | Delicate flavour | |
| Embodiment 1 | 1.0 | 1.4 | 1.3 | 8.4 |
| Embodiment 2 | 1.4 | 1.9 | 2.1 | 8.1 |
| Embodiment 3 | 1.5 | 1.7 | 1.9 | 7.5 |
| Comparative example 1 | 2.9 | 2.8 | 1.9 | 3.2 |
| Comparative example 2 | 5.6 | 6.3 | 3.4 | 2.7 |
As seen from the above table, using the obtained algae peptide combinations of the present invention, with less bitter off-flavors and tart flavour, and delicate flavour
Preferably.
2. free radical scavenging activity (DPPH methods)
The algae peptide combinations that embodiment 1-3 and comparative example 1-2 are obtained are dissolved in absolute ethyl alcohol, with DPPH (1,1- diphenyl-
2- trinitrophenyl-hydrazines) ethanol solution for control, at 517nm absorbing wavelengths detect light absorption value, calculate free radical is clear
Except rate, the results are shown in table below：
2 free radical scavenging activity of table
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | |
| Clearance rate [%] | 93.4 | 90.9 | 91.7 | 76.3 | 74.6 |
The above embodiment is only the preferred embodiment of the present invention, it is impossible to limit the model protected of the present invention with this
It encloses, the variation and replacement of any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to the present invention
Scope claimed.
Claims (10)
1. a kind of algae peptide combinations, which is characterized in that it prepares raw material and is formed by mixing algae, egg mix white powder and freshwater mussel meat, institute
State mixing algae be made of chlorella pyrenoidosa, Euglena and salt algae, the egg mix white powder by pea protein, dragon protein and just
Milk basic protein forms.
2. algae peptide combinations as described in claim 1, which is characterized in that the raw material for preparing is by the mixed algae in terms of dry weight
Class, egg mix white powder and freshwater mussel meat press (15-20)：(2.5-3.5)：1 weight ratio composition.
3. algae peptide combinations as described in claim 1, which is characterized in that the mixing algae is small by the pyrenoids in terms of dry weight
Ball algae, Euglena and salt algae press (5-10)：(5-10)：2 weight ratio composition.
4. algae peptide combinations as described in claim 1, which is characterized in that the egg mix white powder is by pea protein, earthworm egg
White and first milk basic protein presses (6-8)：(2-3)：1 weight ratio composition.
5. a kind of preparation method of such as claim 1-4 any one of them algae peptide combinations, comprises the following steps：
1) pre-treatment of algae is mixed：The clean, chlorella pyrenoidosa without sand is chosen, is placed in autoclave, is passed through saturation
90-95 DEG C is steam heated to, 5-10min is maintained, is forced into 1.2-1.4MPa, instantaneously reduces to normal pressure, it is small to obtain broken wall pyrenoids
Ball algae；
2) it is levigate：Euglena and salt algae are added in into the broken wall chlorella pyrenoidosa that step 1) obtains, adds the water of 0.5-5 times of weight,
The sieving rate of 200 mesh sieves was ground to repeatedly in colloid mill up to more than 95%, obtains algae slurries；
3) pre-treatment of freshwater mussel meat：Freshwater mussel meat is taken to be beaten, is freezed, is placed in autoclave, is passed through saturated vapor and is heated to 90-95
DEG C, maintain 5-10min, after be forced into 1.2-1.4MPa, instantaneously reduce to normal pressure, add in the water of 0.5-5 times of weight, homogenate obtains
Freshwater mussel meat liquid；
4) ferment：Bacillus subtilis is accessed into the freshwater mussel meat liquid that step 3) obtains, ferment 10-20min, boils inactivation, obtains
Freshwater mussel meat fermentation liquid；
5) fat enzymolysis：It is mixed to the algae slurries that step 1) obtains with the freshwater mussel meat fermentation liquid that step 3) obtains, adds in lipase, surpassed
Sound assistance enzymolysis when standing 1-2 is small, boils inactivation, after being down to 35-40 DEG C, obtains the first enzymolysis liquid；
6) tryptose digests：Egg mix white powder is added in the first enzymolysis liquid obtained to step 5), adds in trypsase, adjusts pH
To 6.0-6.5, ultrasonic wave added enzymolysis 10-30min when standing 1-2 is small, inactivation is boiled, obtains the second enzymolysis liquid；
7) basic protein digests：Basic protein enzymolysis is added in the second enzymolysis liquid obtained to step 5), adjusts pH to 7.0-
7.5, ultrasonic wave added enzymolysis 10-30min when standing 1-2 is small, boils inactivation, obtain the second enzymolysis liquid；
8) bitter off-flavors are taken off：The pH of enzymolysis liquid is adjusted as 7.0-7.5, adds in the activated carbon of 1-3%, centrifugation takes supernatant；
9) it is dry：Thick liquid is concentrated under reduced pressure into, is spray-dried, obtains algae peptide combinations.
6. preparation method as claimed in claim 5, which is characterized in that in step 3), the addition of the bacillus subtilis
For the 0.5-1% of freshwater mussel jerky weight, fermentation temperature is 42-45 DEG C.
7. preparation method as claimed in claim 5, which is characterized in that in step 5), the enzyme concentration of lipase is raw material gross weight
The 0.1-0.5% of amount, ultrasonic power are 200-300W.
8. preparation method as claimed in claim 5, which is characterized in that in step 6), the enzyme concentration of trypsase is total for raw material
The 2-3% of weight；In step 7), the enzyme concentration of alkali protease is the 2-3% of raw material gross weight.
9. preparation method as claimed in claim 5, which is characterized in that in step 5) and/or step 6) and/or step 7), surpass
The concrete operations of sound assistance enzymolysis are：After ultrasonic 10-30s, suspend 1-3min, then ultrasonic wave added 20-30min.
It is 10. a kind of such as application of the claim 1-4 any one of them algae peptide combinations in healthy food.
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| CN111466499A (en) * | 2020-05-06 | 2020-07-31 | 青岛卓越海洋集团有限公司 | Application of chlorella in preparation of fish culture feed |
| CN116406798A (en) * | 2023-04-17 | 2023-07-11 | 云康大健康产业(广州)有限公司 | Marine bioactive peptide composition and application thereof |
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| CN116406798A (en) * | 2023-04-17 | 2023-07-11 | 云康大健康产业(广州)有限公司 | Marine bioactive peptide composition and application thereof |
| CN116406798B (en) * | 2023-04-17 | 2023-10-03 | 云康大健康产业(广州)有限公司 | Marine bioactive peptide composition and application thereof |
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