CN108077316A - A kind of microbial bacterial agent that rhodiola root is promoted to take root and preparation method thereof - Google Patents
A kind of microbial bacterial agent that rhodiola root is promoted to take root and preparation method thereof Download PDFInfo
- Publication number
- CN108077316A CN108077316A CN201711340501.4A CN201711340501A CN108077316A CN 108077316 A CN108077316 A CN 108077316A CN 201711340501 A CN201711340501 A CN 201711340501A CN 108077316 A CN108077316 A CN 108077316A
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- Prior art keywords
- root
- rhodiola root
- microbial bacterial
- bacterial agent
- rhodiola
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
Abstract
The present invention relates to a kind of microbial bacterial agents that rhodiola root is promoted to take root and preparation method thereof, belong to microbial bacterial agent research and development field.The primary raw material that the microbial bacterial agent uses is Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 etc..Rhodiola root (Rhodiolarosea L.) is a kind of high plant of medical value, utilize agrobacterium rhizogenes, polysorbas20, urea, potassium fulvate, chitosan is configured to microbial bacterial agent by a certain percentage, it is planted applied to rhodiola root, since agrobacterium rhizogenes and rhodiola root form a kind of symbiosis, it have stimulated the branch of rhodiola root root and root growth and increase root absorption area, rhodiola root has been effectively promoted to take root growth, greatly improve rhodiola root quality, raw material best in quality are provided for the further investigation of rhodiola root medical value, there is huge economic benefit.
Description
Technical field
The present invention relates to a kind of microbial bacterial agents that rhodiola root is promoted to take root and preparation method thereof, belong to agricultural technology neck
Domain.
Background technology
Microbial bacterial agent be microorganism by production expand it is numerous after, utilize viable bacteria made of the zymotic fluid of adsorbent absorption thalline
Preparation.This microbial inoculum has for seed dressing or root dipping and directly or indirectly improves soil, recover soil fertility, prevent soil-borne disease, maintain
The effects that rhizosphere microflora balance and degradation poisonous and harmful substances.The appropriate use of agricultural microbial agent can improve agricultural product
Yield improves quality of agricultural product, reduces fertilizer amount, reduce cost, improvement soil, preserve the ecological environment.
The hairy root that agrobacterium rhizogenes (Agrobacterium rhizogenes) induction plant generates can synthesize the plant
Secondary metabolites, while hairy root has growth cycle, condition of culture is simple, stability is strong, purpose product content height etc.
Feature.Therefore, hairy root culture technology is a kind of effective way for effectively and reliably producing synthesis Secondary metabolites, from
New medicinal compound is found in hairy root culturd with potential application value.At present, can be given birth to by the culture of hairy root
The secondary metabolites of production has alkaloids (such as indole alkaloid, quinoline alkaloid, gelsemium henbane alkane alkaloid), glucoside (such as people
Join saponin, beet glucoside etc.), flavonoids, quinones (such as shikonin), polysaccharide, protein (such as trichosanthin) and some
Important biological enzyme (such as superoxide dismutase).According to incompletely statistics, 23 sections more than 50 kind medicinal plant is carried out both at home and abroad
The research of hairy root induction, establishes long-term hairy root culture systems, obtains secondary metabolite.Rhodioloside conduct
A kind of most effective active material in rhodiola root often evaluates rhodiola root in practical study with the content of rhodioloside height
Quality.Modern pharmacology research shows that rhodioloside has resist oxygen lack, anti-aging, antifatigue, resisting microwave radiation, adjusting
Immune function promotes the effects that vivo protein metabolism, removing toxic substances, and has dual regulation to blood pressure, blood glucose.Therefore, cultivate
Go out the rhodiola root plant of high quality, it is made to play greatest benefit, be the Main way for cultivating rhodiola root.
The content of the invention
For above-mentioned phenomenon, it is an object of the invention to utilize the distinctive work for promoting plant root growth of agrobacterium rhizogenes
With so as to increase the accumulation of rhodiola root secondary metabolite rhodioloside, providing and a kind of promote the microorganism that takes root of rhodiola root
The preparation method of microbial inoculum.
The present invention is realized by following technical scheme：
A kind of microbial bacterial agent for promoting rhodiola root growth, which is characterized in that the microbial bacterial agent is by following component
Raw material proportioning be made：
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more of 15834, strain derives from Chinese microorganism strain collection；
It is on the market 99% to spit by the content of Beijing Ji Mei bio tech ltd production that the Tween-20, which is,
Temperature -20；
The urea that it is on the market 99.9% by the content of Beijing Kang Puhui dimensions Science and Technology Ltd. production that the urea, which is,；
The potassium fulvate is the potassium fulvate of content >=70% by the production of Jinan Rong Guan Co., Ltds on the market；
The chitosan is that the active principle content produced on the market by hundred taste bio tech ltd of Hebei is
99.8% chitosan.
The agrobacterium rhizogenes bacterium solution is the Agrobacterium bacterium solution that OD values are 0.4-0.6.
The dodecyl sodium sulfate added in microbial inoculum is surfactant, can promote absorption of the plant root to microbial inoculum.
A kind of preparation process of microbial bacterial agent that rhodiola root is promoted to take root is：
By agrobacterium rhizogenes strain A4, R1205, R1000, R1601, R1022, one or more of 15834 activation extremely
OD values are 0.4-0.6, take the bacterium solution after activation, and the bacterium solution 50ml after activation is separately added into Tween-20 1g, urea 20g, yellow corruption
Sour potassium 8g, chitosan 10g, mixing obtain microbial bacterial agent.
The present invention has following positive effect：
1st, the microorganism fungus kind that microbial bacterial agent of the present invention uses for agrobacterium rhizogenes strain A4, R1205, R1000,
R1601, R1022, one or more of 15834, agrobacterium rhizogenes generate symbiosis with rhodiola root root, make its indefinite
Root growth is rapid, finally develops into hairy root, has cultivated a kind of rhodiola root plant of well developed root system.
2nd, microbial bacterial agent of the present invention uses agrobacterium rhizogenes bacterium solution, and the components such as urea, Tween-20 are relatively low with cost,
The advantages of easily acquisition, preparation method is simple, it is readily put into production.
3rd, the preparation method for the microbial bacterial agent that a kind of promotion rhodiola root of the invention is taken root, has been effectively promoted rhodiola root
It takes root growth, provides raw material best in quality for the further investigation of rhodiola root medical value, there is huge economic benefit.
Specific embodiment
With reference to case study on implementation, the invention will be further described
It is a kind of promote rhodiola root growth microbial bacterial agent, the microbial bacterial agent by following component raw material proportioning system
Into：
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more of 15834, strain derives from Chinese microorganism strain collection；
It is on the market 99% to spit by the content of Beijing Ji Mei bio tech ltd production that the Tween-20, which is,
Temperature -20；
The urea that it is on the market 99.9% by the content of Beijing Kang Puhui dimensions Science and Technology Ltd. production that the urea, which is,；
The potassium fulvate is the potassium fulvate of content >=70% by the production of Jinan Rong Guan Co., Ltds on the market；
The chitosan is that the active principle content produced on the market by hundred taste bio tech ltd of Hebei is
99.8% chitosan.
The agrobacterium rhizogenes bacterium solution is the Agrobacterium bacterium solution that OD values are 0.4-0.6.
The dodecyl sodium sulfate added in microbial inoculum is surfactant, can promote absorption of the plant root to microbial inoculum.
A kind of preparation process of microbial bacterial agent that rhodiola root is promoted to take root is：
By agrobacterium rhizogenes strain A4, R1205, R1000, R1601, R1022, one or more of 15834 activation extremely
OD values are 0.4-0.6, and the bacterium solution 50ml after activation is separately added into Tween-20 1g, urea 20g, yellow corruption by the bacterium solution after must activating
Sour potassium 8g, chitosan 10g, mixing obtain microbial bacterial agent.
Promote the application process of rhodiola root growth
The rhodiola root seedling in tri-leaf period is taken to plant in soil, microbial bacterial agent is imposed on into rhodiola root seedling, every plant of about 50ml is dilute
Release liquid (extension rate：20), microbial inoculum is applied once within every 15 days.It is fast with apparent growth and development in rhodiola root plant developmental process,
The characteristics of well developed root system, cauline leaf growth is fast, and survival rate of plant is high.
Application of the bacteria agent in rhodiola root growth based on agrobacterium rhizogenes
20 plants of the rhodiola root seedling in tri-leaf period, is divided into control group and experimental group by every group each 10 plants, and control group does not apply microbial inoculum,
Experimental group applies microbial inoculum, every plant of about 50ml dilution (extension rate：20), apply microbial inoculum once within every 15 days, apply microbial inoculum altogether 3 times, observe
Record Reducing sugar.The results are shown in table below for record：
Different number of days rhodiola root growing state
The result shows that the rhodiola root for being applied with microbial bacterial agent has in developmental process, apparent survival rate is high, root system
Prosperity, eugonic feature, the plant for less applying microbial bacterial agent are compared, and growth quality is obviously improved, result explanation, this
Inventing a kind of microbial bacterial agent that rhodiola root is promoted to take root improves the quality of rhodiola root, is the further of rhodiola root medical value
Exploitation provides raw material best in quality.
Table 1 is at different number of days (15d, 30d, 45d), does not apply microbial inoculum rhodiola root (control group) of the present invention and applies this hair
The plant height of bright microbial inoculum rhodiola root (experimental group), root long, root weigh and the variation of rhizome number.
Table 1 applies the influence that microbial inoculum grows rhodiola root
Note：At different number of days (15d, 30d, 45d), do not apply microbial inoculum rhodiola root (control group) and apply microbial inoculum rhodiola root
The plant height of (experimental group), root long, root weigh and the variation of rhizome number.
The variation of the growth phenotype of the rhodiola root plant of microbial inoculum is less applied by the rhodiola root plant for measuring application microbial inoculum, is obtained
The data arrived further illustrate that be applied with has apparent well developed root system with the rhodiola root of microbial inoculum in developmental process, and growth is prosperous
The characteristics of Sheng, the plant for less applying microbial bacterial agent are compared, and growth quality is obviously improved.A kind of promotion rhodiola root of the present invention is taken root
Microbial bacterial agent improve the quality of rhodiola root, further improve rhodiola root medical value.
Claims (4)
1. a kind of microbial bacterial agent that rhodiola root is promoted to take root, which is characterized in that the bacteria agent is matched by following component
Raw material be made：
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more of 15834, strain derives from Chinese microorganism strain collection；
The Tween-20 that it is on the market 99% by the content of Beijing Ji Mei bio tech ltd production that the Tween-20, which is,；
The urea that it is on the market 99.9% by the content of Beijing Kang Puhui dimensions Science and Technology Ltd. production that the urea, which is,；
The potassium fulvate is the potassium fulvate of content >=70% by the production of Jinan Rong Guan Co., Ltds on the market；
The chitosan is that the active principle content produced on the market by hundred taste bio tech ltd of Hebei is
99.8% chitosan.
2. a kind of preparation method of microbial bacterial agent that rhodiola root is promoted to take root according to claim 1, it is characterised in that：
The agrobacterium rhizogenes bacterium solution is the Agrobacterium bacterium solution that OD values are 0.4-0.6.
3. a kind of preparation method of microbial bacterial agent that rhodiola root is promoted to take root according to claim 1, which is characterized in that
The Tween-20 added in microbial inoculum is surfactant, can promote absorption of the plant root to microbial inoculum.
4. a kind of preparation method of microbial bacterial agent that rhodiola root is promoted to take root according to claim 1, which is characterized in that
Its preparation process is：
By agrobacterium rhizogenes strain A4, R1205, R1000, R1601, R1022, one or more of 15834 activation to OD values
For 0.4-0.6, the bacterium solution after activation is taken, the bacterium solution 100ml after activation is separately added into Tween-20 1g, urea 20g, fulvic acid
Potassium 8g, chitosan 10g, mixing obtain microbial bacterial agent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711340501.4A CN108077316A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial bacterial agent that rhodiola root is promoted to take root and preparation method thereof |
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| CN201711340501.4A CN108077316A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial bacterial agent that rhodiola root is promoted to take root and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757519A (en) * | 2018-12-27 | 2019-05-17 | 金华市胤宏农业科技有限公司 | A kind of rose transplantation rooting agent composition |
| CN109874628A (en) * | 2018-12-27 | 2019-06-14 | 金华市泽雨园艺技术有限公司 | A kind of cultural method of Monochoria korsakowii |
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| CN103081950A (en) * | 2011-11-04 | 2013-05-08 | 天津绿动植物营养技术开发有限公司 | Biological agent type rooting liquid |
| CN103183567A (en) * | 2013-04-01 | 2013-07-03 | 张元宝 | Liquid bacterial agent for rooting |
| CN104604503A (en) * | 2015-02-04 | 2015-05-13 | 浙江大学 | Method for root rot resistance and growth promotion of rhodiola rosea through biological prevention and control |
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2017
- 2017-12-14 CN CN201711340501.4A patent/CN108077316A/en active Pending
Patent Citations (4)
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| CN101361446A (en) * | 2008-10-15 | 2009-02-11 | 四川省川草工程咨询招标有限责任公司 | Rhodiola seed seedling-raising method and cultivation method |
| CN103081950A (en) * | 2011-11-04 | 2013-05-08 | 天津绿动植物营养技术开发有限公司 | Biological agent type rooting liquid |
| CN103183567A (en) * | 2013-04-01 | 2013-07-03 | 张元宝 | Liquid bacterial agent for rooting |
| CN104604503A (en) * | 2015-02-04 | 2015-05-13 | 浙江大学 | Method for root rot resistance and growth promotion of rhodiola rosea through biological prevention and control |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757519A (en) * | 2018-12-27 | 2019-05-17 | 金华市胤宏农业科技有限公司 | A kind of rose transplantation rooting agent composition |
| CN109874628A (en) * | 2018-12-27 | 2019-06-14 | 金华市泽雨园艺技术有限公司 | A kind of cultural method of Monochoria korsakowii |
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