The method that the healthy and strong seedling of granule crop is cultivated using compound soil matrix culture material rapid, high volume
Technical field
The present invention relates to technical field of plant cultivation more particularly to a kind of granule crop be a large amount of, healthy and strong seedling is simply cultivated
Soil matrix and related breeding method.
Background technology
In crop science research, many experiments are both needed to using seedling as research object.In basic research, genomic DNA
Extraction, the extraction of chloroplaset, the extraction of mitochondria be required to a large amount of, the seedling leaves tissue of high quality;And the granules such as millet
, it is necessary to identify the purity of cenospecies in the application studies such as crop hybrid kind popularization, still, in order not to delay farming season, do not influence secondary
Year sowing, the Purity of cenospecies needs in winter or early spring carries out, also with regard to needs in temperature higher interior or greenhouse
Carry out, the granules such as millet crop hybrid kind is mostly two-series hybrid at present, wherein male parent -- a kind of anti-herbicide of restorer, and this
Kind anti-herbicide gene is maternal in dominant inheritance -- and sterile line has certain self-fertility characteristic, and not antiweed or anti-
Another herbicide, cenospecies is consistent with restorer to the resistance of herbicide or resists two kinds of herbicides, therefore, granule crop simultaneously
The Purity method of two-series hybrid is exactly that the herbicide that male parent resists is sprayed in the 4 leaf phase of seedling, is killed except female parent seedling therein, i.e.,
Pseudostationary, then calculates the purity of cenospecies by counting the ratio of resistance seedling, and the Purity of cenospecies is also required to cultivate big
The seedling of amount.Therefore, how to cultivate a large amount of, healthy and strong seedling becomes one of key link that can various experiments successful.
The method for culturing seedlings of granule crop mainly has culture dish germination test method, container soil property moisture saturation state to issue at present
Bud test method(s), sand culture germination nursery, container nursery, flowerpot nursery, seedlings nursing plate (bunch planting) nursery that vermiculite is matrix and nutrient solution,
Nutrition Soil seedling method etc..In above-mentioned seedling method, culture dish sproutung method and container soil property water saturation germination test method are mainly used for
The germination percentage of seed is measured, seedling is thin and delicate after germination, can only meet needs of the special small trials to leaf tissue;Sand culture seedling method
The seedling of cultivation is small and weak, second-rate, can only also meet the needs of small trials;Container nursery method is cultivated using special container
Crop, fruit tree, flowers, the seedling raising manners of forest seedling, common container for plant growth are to fill the matrix such as abundant nutrient compost
Nutritive cube, since container volume is small, container seedling generally all shallow stems of root are short, and easily form root system deformity, and seedling is small weak, and seedling amount
It is small, it is impossible to meet needs of the experiment to a large amount of seedling;Flowerpot nursery and seedlings nursing plate nursery are mostly vermiculite or battalion due to matrix used
It supports soil and area is small, it is bothersome to manage, it is also difficult to a large amount of, the healthy and strong seedling of high quality is cultivated, it is fine for small trials, it is right
It is difficult then the needs for meeting experiment in the experiment of a large amount of seedling of needs such as chloroplaset, mitochondria extraction and Purity;Nutrient solution
Nursery, it is necessary to preferable equipment, of high cost, and is operated more demanding due to well-off nutrient and oxygen supply, it is also difficult to
Cultivate a large amount of healthy and strong seedling that disclosure satisfy that experiment needs.
Therefore, be badly in need of establishing a set of method for culturing seedlings that can cultivate a large amount of, healthy and strong seedling, for carry out the extraction of genomic DNA,
The extraction of chloroplaset, the extraction of mitochondria and granule crop hybrid kind Purity provide technical support, while also deep to carry out
The research of level provides basic technical support.
The content of the invention
The technical problem to be solved by the present invention is to overcome a variety of deficiencies of the prior art, provide a kind of for rapid, high volume training
It educates the compound soil matrix culture material of the healthy and strong seedling of granule crop and utilizes above-mentioned culture material rapid, high volume cultivation granule crop healthy and strong
The method of seedling.
In order to solve the above technical problems, the technical solution used in the present invention is as follows.
The compound soil matrix culture material of the healthy and strong seedling of granule crop is cultivated for rapid, high volume, the culture material is as follows
It prepares:
A, the preparation of " base soil a ":Take crop field loam first, it is outdoor spread air-dried 7 days out after, " the base soil as soil matrix nursery
a”;
B, the preparation of " base soil b ":" base soil a " is laid in seedling-raising box, pour water into seedling-raising box while is stirred evenly, directly
Reach saturation state to " base soil a " water content, through precipitating and pouring out surface layer excessive moisture, be made " base soil b ";
C, the preparation of " soil matrix A ":" base soil b " and " base soil a " are weighed in proportion, and then " base soil a " is added in " base soil b "
And it stirs evenly to get " soil matrix A ";
D, the preparation of " soil matrix B ":" soil matrix A " and " base soil a " are weighed in proportion, " soil matrix A " arrangement is shakeout, and then will
" base soil a " tiling to the upper surface of " soil matrix A ", standing treat " base soil a " stablize spread and sink in it is wet to get " soil matrix B ", it is upper it is dry under wet nursery soil
Prepared by earth completes.
As a preferred technical solution of the present invention, in step C, the weight of " base soil b " and " base soil a " in " soil matrix A "
Portion rate is (1.5-2):1;At the same time, in step D in gained " soil matrix B ", the ratio of weight and number of " base soil b " and " base soil a "
For (1.5-3.5):1.
As a preferred technical solution of the present invention, in step C, the weight of " base soil b " and " base soil a " in " soil matrix A "
Portion rate is 1.5:1;At the same time, in step D in gained " soil matrix B ", the ratio of weight and number of " base soil b " and " base soil a " are
(2.5-3.0):1。
As a preferred technical solution of the present invention, in step C, the weight of " base soil b " and " base soil a " in " soil matrix A "
Portion rate is 2:1;At the same time, in step D in gained " soil matrix B ", the ratio of weight and number of " base soil b " and " base soil a " is (1.5-
3.5):1。
As a preferred technical solution of the present invention, in step C, the weight of " base soil b " and " base soil a " in " soil matrix A "
Portion rate is 2:1;At the same time, in step D in gained " soil matrix B ", the ratio of weight and number of " base soil b " and " base soil a " is 2.5:
1。
The method for cultivating the healthy and strong seedling of granule crop using above-mentioned compound soil matrix culture material rapid, high volume includes following step
Suddenly:
Ith, " base soil a " and common water for irrigation is utilized to prepare higher " the soil matrix A " of bottom aqueous amount;
IIth, " soil matrix B " seedling raising environment wet under being done on the soil matrix structure of step I is utilized;
IIIth, controllable type sowing is carried out in the seedling raising environment of step II;
IVth, the seedling management of systematization is after planting carried out, so as to the substantial amounts of healthy and strong seedling of quick obtaining.
As a preferred technical solution of the present invention, step I includes as follows step by step:
I -1, the selection and processing of soil matrix:Fertile crop field loam is chosen, it is outdoor to spread out, air-dry 7 days, it obtains as nursery
" the base soil a " of matrix;
I -2, the selection of seedling-raising box:According to the requirement to seedling, choose length, width and height be respectively 40-60cm, 30-50cm,
The seed turnover box of 10-30cm or the sizes such as other and the consistent upper open box of upper and lower bore is as seedling-raising box;
I -3, the preparation of base portion water saturation " base soil b ":" the base soil a " for preparing in I -1 is laid in seedling-raising box, thickness
Then 3-5cm pours water and stirs evenly into seedling-raising box, until soil moisture content up to saturation state, then fully precipitates and will
The extra moisture in soil matrix surface layer is poured out, and stores sufficient base portion moisture to get " base soil b ";
I -4, the preparation of bottom aqueous amount higher " soil matrix A ":According to base soil b:Base soil a is " 1.5:1、2.0:1 " 2 kind is matched somebody with somebody
Than " base soil a " is added in " the base soil b " that is configured in step I -3, and mix uniformly, that is, be configured to " soil matrix A ".
As a preferred technical solution of the present invention, the concrete operation method of step II is:It is germinateed according to granule crop
Law of needing the water and bottom " soil matrix A " water content, determine that " soil matrix A " covers the ratio of " base soil a " above, then, stand 2 small
When, since the moisture in bottom " soil matrix A " is constantly returned to upper strata " base soil a ", " the base soil a " on wet upper strata of spreading and sinking in makes upper strata also possess
Certain water content;Specifically pattern is matched comprising the following two kinds:
II -1, base soil b in " soil matrix A ":Base soil a=1.5:When 1, upper strata is according to base soil b:Base soil a=2.5:1 or 3.0:1
It covers " base soil a ", when standing 2 is small, obtains " soil matrix B ";
II -2, base soil b in " soil matrix A ":Base soil a=2:When 1, upper strata is according to base soil b:Base soil a=1.5:1、2.0:1、
2.5:1、3.0:1、3.5:1 covering " base soil a ", when standing 2 is small, obtains " soil matrix B ".
As a preferred technical solution of the present invention, step III includes as follows step by step:
III -1,2-4 row ditches are drawn with small wooden in each box, by the uniformly sowing of 80-120/row;
III -2, after planting, small operculum colpi will be sowed with " base soil a " to put down, and will pat soil matrix surface layer with hand, seed box soil matrix is made to try one's best
It is tightly combined, is placed in greenhouse or growth room, outside late spring, summer or early autumn direct holding chamber.
As a preferred technical solution of the present invention, step IV includes as follows step by step:
IV -1, after emerging, according to paddy seedling growing way, every 3 days, morning 8-10 points were quantitatively sprayed water with small watering can, each seedling-raising box
Each moisturizing 1-2 liters to prevent topsoil backbone from splitting, ensure that paddy seedling grows vigorously use;
IV -2,4 leaf phases, blade can be used as extraction inhereditary material or spray identification purity of hybrid;
IV -3,5 leaf phases added the fertilising concentration of 10 grams of urea according to every 1.5 liters of water, and urea is dissolved in the water, during with moisturizing
It applies in seedling-raising box, that is, obtains healthy and strong seedling.
It is using advantageous effect caused by above-mentioned technical proposal:The present invention constructs a set of energy rapid, high volume cultivation and grows sturdily
The architecture method for culturing seedlings of strong seedling, to carry out the extraction of genomic DNA, the extraction of chloroplaset, the extraction of mitochondria and granule
Crop hybrid kind Purity provides technical support, while also provides basic technical support to carry out profound research.
One aspect of the present invention according to crop Water uptake regulation, create a kind of lower substrate moisture it is higher, on not substrate water
Divide content relatively low, beneficial to pricking, absorbing water under crop root, so as to create a kind of environmental condition beneficial to seedling robust growth, make children
Seedling being capable of healthy and strong, healthy growth;Simultaneously according to all nutrients needed in soil containing plant growth especially seedling growth, and
Water holding capacity is good, using soil as matrix, compared with quick, the healthy growth that the matrix such as vermiculite and Nutrition Soil, water are more conducive to crop seedling,
It is just more conducive to cultivate a large amount of health, healthy and strong seedling.
Inventor project team has utilized method provided by the invention, is successfully made the cultivation of millet seedling, experiment and examination
It is very prominent (specific embodiment that test example see below) with effect, to carry out inhereditary material extraction and millet double-line hybrid
Solid foundation has been established in the correlative studys such as kind Purity.
Description of the drawings
Fig. 1 is to cultivate 4 leaf phase of millet, 6 leaf phases, 8 leaf phase growing ways using the method for the present invention in embodiment;From left-hand in figure
The right side is followed successively by 4 leaf phase growing ways, 6 leaf phase growing ways, 8 leaf phase growing ways.
The method that Fig. 2 is the 8 leaf phase present invention in embodiment cultivates millet growing way compared with turf, nutrition pot grown seedling;In figure, most
Lower row is from left to right followed successively by the method for the present invention, turf seedling method, Nutrition Soil seedling method and 8 leaf phase of the method for the present invention growing way.
Fig. 3 is the 8 simple soil matrix nursery of leaf phase different ratio compared with turf, Nutrition Soil nursery effect.
Specific embodiment
The present invention is described in detail in following embodiment.Various raw materials used in the present invention and items of equipment are conventional city
Product is sold, can be bought and directly obtained by market.
All professors Liu Zhengli of seed-grain used preside over 32 seed of Ji paddy being bred as in embodiment;The all earth of base soil used
Soil is disposably taken at Tangshan Teachers College experimental plot;Water used is ordinary tap water;Urea used is purchased from means of agricultural production retail sales, control
Turfy soil used, Nutrition Soil are purchased from flowers market;Soil drying is using weight method:The weight in wet base M of soil sample is recorded, in 105 DEG C of baking
Soil sample is dried 6-8 hour to constant weight in case, then measures soil sample, record soil sample dry weight Ms,
Plant and root system furnace drying method are baked to (first when 1050C water-removings 0.5 are small, then go in an oven
800C dries to constant weight when small (about 24)).
The preparation of the higher soil matrix of embodiment 1, bottom aqueous amount.
(1) measure of the selection of soil matrix and base soil water content:More fertile earth is chosen from Tangshan Teachers College experimental plot
Soil is as nursery base soil;It is outdoor to spread out, after air-drying 7 days, for soil matrix nursery;And it by 3 repeated samplings, is measured using oven drying method
The average moisture content of " base soil a " is 6.84%.
(2) selection of seedling-raising box:Long 50cm, width 40cm, the seed turnover box of high 20cm are chosen as seedling-raising box.
(3) preparation of base portion water saturation " base soil b ":The base soil that (1) prepares is laid in seedling-raising box, thickness about 3-
5cm (according to the specific thickness of ratio-dependent of addition base soil a), then pours water into seedling-raising box, and constantly mixes base soil with wooden stick
And water, soil group moisture content is made to be in uniform state, after base portion soil group moisture content reaches saturation state, it is allowed to precipitate 15 minutes
Afterwards, the extra moisture in soil matrix surface layer is poured out, stores sufficient base portion moisture, be " base soil b ".
(4) preparation and its moisture determination of bottom aqueous amount higher " soil matrix A ":According to " base soil b:Base soil a " is
“0.5:1、1.0:1、1.5:1、2.0:1、2.5:1、3.0:1、3.5:1、4.0:" base soil a " is added to step (3) by 1 " 8 kind of proportioning
In " the base soil b " of configuration, and mix uniformly, that is, be configured to " soil matrix A ".After " soil matrix A " is prepared, by 3 repeated samplings, use
Oven drying method, measures the water content of " soil matrix A ", and " soil matrix A " water content respectively matched is shown in Table 1.
The water content (%) of 1 different ratio of table " soil matrix A "
Code name |
Base soil b:Base soil a |
Ⅰ |
Ⅱ |
Ⅲ |
It is average |
1 |
0.5:1 |
23.15 |
29.84 |
23.53 |
25.51 |
2 |
1.0:1 |
48.37 |
49.35 |
48.00 |
48.58 |
3 |
1.5:1 |
58.14 |
58.94 |
57.92 |
58.33 |
4 |
2.0:1 |
63.54 |
62.96 |
63.56 |
63.35 |
5 |
2.5:1 |
70.51 |
69.11 |
70.80 |
70.14 |
6 |
3.0:1 |
76.84 |
76.62 |
77.25 |
76.90 |
7 |
3.5:1 |
78.28 |
78.68 |
77.95 |
78.30 |
8 |
4.0:1 |
79.21 |
80.28 |
80.01 |
79.83 |
Embodiment 2, it is upper it is dry under wet seedling raising environment preparation.
(1) preparation and its moisture determination of upper strata water content relatively low " soil matrix B ":Continue according to " base soil b:Base soil a "
Respectively " 0.5:1、1.0:1、1.5:1、2.0:1、2.5:1、3.0:1、3.5:1、4.0:1 " ratio is put down above at " soil matrix A "
It spreads " base soil a ", when standing 2 is small after having tiled, prepares serial " soil matrix B ".
(2) in this way, it is upper it is dry under wet seedling medium " soil matrix B " prepare successfully, middle and lower part " soil matrix A " includes that " base is native
b:Base soil a is respectively 0.5:1、1:1、1.5:1、2.0:1、2.5:1、3.0:1、3.5:1、4.0:1 " 8 kind of processing, code name are respectively
“1、2、3、4、5、6、7、8”;Each " soil matrix A " above Casing ways again include " base soil b:Base soil a is respectively 0.5:1、1:1、
1.5:1、2.0:1、2.5:1、3.0:1、3.5:1、4.0:1 " 8 kind of processing, code name are respectively " 1,2,3,4,5,6,7,8 ", so
Formed " 11,12,13,14,15,16,17,18,21 ..., 86,87,88 " in 64 " soil matrix B " match.Then, from " soil matrix B "
3 repetition grab sample are pressed on surface layer, measure " soil matrix B " surface layer water content, and specific measurement result is shown in Table 2.
The water content (%) on 2 different ratio of table " soil matrix B " surface layer
Embodiment 3, seeding method.
(1) 3 row ditches are drawn with small wooden in each box, it is each to locate by 100/row (i.e. per 300, box) uniformly sowing
Reason 3 boxes of plantation.
(2) after planting, small operculum colpi will be sowed with " base soil a " to put down, soil matrix surface layer is patted with hand, seed box soil matrix is made to tie as far as possible
It closes closely, this experiment is carried out in August, 2017, is placed directly in outdoor.
Embodiment 4, seedling management method.
(1) after emerging, according to paddy seedling growing way, every 3 days, 9 points or so of the morning was quantitatively sprayed water once with small watering can, each nursery
1.5 liters of each moisturizing of box to prevent topsoil backbone from splitting, ensures that paddy seedling grows vigorously use.
(2) fresh weight, dry weight, the root that 40 plants of paddy seedlings is respectively taken to measure paddy seedlings are each handled respectively at 4 leaf phases, 6 leaf phases, 8 leaf phases
It is length and weight of root system.
(3) by the comparison of the indexs such as plant dry weight, weight of root system and root length, determine that the optimal of each layer soil is matched somebody with somebody
When respectively step water content standard, each processing emergence situation and seedling growth are shown in Table 3, table 4, table 5, table 6.
Each serial base soil of table 3 matches situation of after planting emerging
Each serial base soil 4 leaf phase growth of seedling situations of proportioning that table 4 is normally emerged
Each serial base soil 6 leaf phase growth of seedling situations of proportioning that table 5 is normally emerged
Each serial base soil 8 leaf phase growth of seedling situations of proportioning that table 6 is normally emerged
(4) result of study shows:Root/shoot ratio is the leading indicator for judging whether seedling is healthy and strong, is secondly dry matter weight of aerial parts
Weight, the result of comprehensive two aspects:“26、27、35、36、43、44、45、46、47、53、54、55、63、64、73、74、82”
17 kinds of series proportionings are relatively suitble to cultivate millet seedling, wherein " 35,36,43,44,45,46,47 " 7 kinds of proportioning optimums are cultivated
Strong sprout.
Wherein, especially No. 45 proportioning, is respectively provided with outstanding advantage in all fields, the soil matrix breeding recommended as first choice
Formula has extensive application value.
Foregoing description only proposes as the enforceable technical solution of the present invention, not as to its technical solution single in itself
Restrictive condition.