CN108060086A - A kind of preparation method of egg capsule algae special culture media and the purebred liquid of egg capsule algae - Google Patents
A kind of preparation method of egg capsule algae special culture media and the purebred liquid of egg capsule algae Download PDFInfo
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Abstract
The present invention relates to aquaculture water quality regulation and control and water body environment Treatment process fields, more particularly to the preparation method of a kind of egg capsule algae special culture media and the purebred liquid of egg capsule algae, the preparation method is using the pure oocysts algae isolated in high yield shrimp culture pond, it is seeded in special culture media, temperature be 23~28 DEG C, continuous charge culture purified under conditions of 1800~2000Lx of illumination, when egg capsule concentration of algae reaches 65 75 ten thousand/ml after 12~18 days, stop inflation, venting supernatant liquor after precipitation obtains the purebred liquid of egg capsule algae.Compared with prior art, the present invention cultivates for egg capsule algae and provides the physical and chemical factors breeding conditions such as a kind of special nutritional formula and optimum temperature, salinity, acid-base value, illumination, and the prepared purebred liquid of egg capsule algae can be used for selection and breeding and cultivation high activity and the strong egg capsule algae of resistance.
Description
Technical field
The present invention relates to aquaculture water quality regulation and control and water body environment Treatment process fields, and in particular to a kind of egg capsule algae is special
With the preparation method of culture medium and the purebred liquid of egg capsule algae.
Background technology
Egg capsule algae (Oocystis) belongs to Chlorophyta (Chlorophyta), and ball algae guiding principle (Trebouxiophyceae), small altogether
Ball Cutleriales (Chlorellales), egg capsule algae section (Oocystaceae).Plant is spherical or ellipse spherical group
Body, group are typically to be made of 2,4 or 8 cells, the colloid coating for having one layer of pectic substance to form outside group.Frustule is ellipse
Circular or oval, cell is 10-19 μm long, 9-13 μm wide, generates 2,4,8 or 16 autospores under normal circumstances.Egg capsule
Algae is distributed mainly in the water body rich in organic matter such as river mouth and aquaculture pond, has duration length and population stabilization etc.
Feature can reduce the concentration of the injurious factors such as ammonia nitrogen, nitrite in water body, the dissolved oxygen in water be improved, so that water environment
In benign equilibrium state, inhibit the growth of vibrios.
Current prawn culturing mode mainly using the seawater such as industrial aquaculture pond, feed coefficient, Tu Chi or brackish water aquaculture as
It is main.Shrimp culture industry is the pillar industry of China's mariculture industry, and China is subject to always during prawn culturing is developed
The threat of water pollution and shrimp disease problem.Regulate and control the structure and function of microbiologic population in Cultivated water by biotechnology
Come water quality treatment and prevention disease, be the important content of prawn culturing ecology diseases prevention.Egg capsule algae is widely present in southern prawn and supports
Pond is grown, population is stablized, adaptable, and controllable and improvement breeding environment simultaneously prevents disease of prawn.
Traditional egg capsule algae training method is using open cement pit culture, and culture scale is big, and yield is high, but shortcoming
It is：(1) it is easy to pollute, it takes up a large area, is influenced greatly, to be not easy to control by weather, and frustule is slow-growing, the doubling time is long,
One-time investment low output greatly, is not suitable for the expanding species stage；(2) the egg capsule algae algae that traditional approach is cultivated mutually is not sufficiently stable,
Easy to aging and algae, is used for prawn when cultivating pools particularly cultivate the middle and later periods, it may appear that pond water quality is unstable, algae phase
It is unfavorable for the growth of prawn, the phenomenon of falling algae occurs, dead algae can generate a large amount of algae toxins, and then cause the marine products such as prawn
Death；(3) the egg capsule algae that traditional approach is cultivated is difficult to be stored and transported for a long time at normal temperatures, is caused to using
It is inconvenient.These cause egg capsule algae to be difficult to be applied on a large scale in actual production.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of egg capsule algae for being most appropriate to the growth of egg capsule algae
Special culture media and the preparation method that the purebred liquid of egg capsule algae is obtained using the culture medium, the prepared purebred liquid of egg capsule algae can be used for
Selection and breeding and cultivate high activity and the strong egg capsule algae of resistance.
To achieve these goals, the present invention adopts the following technical scheme that：
A kind of egg capsule algae special culture media is provided, is made of the component of following proportioning：
The proportion of the egg capsule algae special culture media is 1.010~1.016, and pH value is 8.0~8.4.
In above-mentioned technical proposal, the egg capsule algae special culture media is made of the component of following proportioning：
In above-mentioned technical proposal, the preparation method of the antiseptic sea water is：Seawater is filtered through Tao Shi hyperfiltration device
Afterwards, 15~25ppm of strong chlorine oil that available chlorine content is 50% is added in, inflation 2h makes drug fully dissolve, then stops inflation, sinks
After forming sediment for 24 hours, add in sodium thiosulfate 5~10ppm and remaining chlorine, and with the chlorine in starch potassium iodide liquid measure antiseptic sea water
Content controls in antiseptic sea water and does not remain effective chlorine composition.
The present invention also provides a kind of preparation methods of the purebred liquid of egg capsule algae, comprise the following steps：
Step 1: the separation that egg capsule algae is purebred
By gathering out egg capsule algae sample in high yield shrimp culture pond, then being separated under microscope and choosing egg capsule frustule；
Step 2: the cultivation of pure oocysts algae
The egg capsule frustule that step 1 is chosen, is seeded in above-mentioned egg capsule algae special culture media, temperature for 23~
28 DEG C, continuous charge culture purified under conditions of 1800~2000Lx of illumination, egg capsule concentration of algae reaches 65-75 ten thousand after 12~18 days
During a/ml, stop inflating, venting supernatant liquor after precipitation obtains the purebred liquid of egg capsule algae.
The beneficial effects of the invention are as follows：
The preparation method of the pure oocysts algae culturing liquid of the present invention is using the egg capsule isolated in high yield shrimp culture pond
Algae is purebred, is seeded in special culture media, is 23~28 DEG C, is continuously filled under conditions of 1800~2000Lx of illumination in temperature
Gas culture purified when egg capsule concentration of algae reaches ten thousand/ml of 65-75 after 12~18 days, stops inflation, and venting upper strata is clear after precipitation
Liquid obtains the purebred liquid of egg capsule algae；Wherein special culture media is (including potassium nitrate, phosphorus by antiseptic sea water 1000ml and nutrient
Acid dihydride potassium, ferric trichloride, vitamin B1, vitamin B12, amino acid training algae element, plant cytokinin, the life of 920 plants
Long hormone) it is made, it is cultivated for egg capsule algae and the physical and chemical factors such as a kind of special nutritional formula and optimum temperature, salinity, acid-base value is provided
Breeding condition, the prepared purebred liquid of egg capsule algae can be used for selection and breeding and cultivates high activity and the strong egg capsule algae of resistance, the egg capsule algae
Algae is mutually stable, non-aging and the advantages of falling algae, and the water quality of aquaculture pond can be kept to be in benign equilibrium state for a long time, so as to
Be conducive to the growth of the marine products such as prawn, improve cultured output and product quality, improve culture benefit.
Specific embodiment
The invention will be further described with the following Examples.
Embodiment 1：
A kind of preparation method of the purebred liquid of egg capsule algae, comprises the following steps：
Step 1: the separation that egg capsule algae is purebred
By gathering out egg capsule algae sample in high yield shrimp culture pond, then being separated under microscope and choosing egg capsule frustule.
Step 2: the cultivation of pure oocysts algae
(1) according to the following ratio, egg capsule algae special culture media is prepared：
The proportion of prepared egg capsule algae special culture media is 1.011, pH value 8.1.
Wherein, the preparation method of antiseptic sea water is：By seawater after the filtering of Tao Shi hyperfiltration device, add in effective chlorine and contain
The strong chlorine oil 20ppm for 50% is measured, inflation 2h makes drug fully dissolve, then stops inflation, after precipitating for 24 hours, adds in thio sulphur
In sour sodium 7ppm and remaining chlorine, and the chlorinity in antiseptic sea water is measured with starch potassium iodide liquid, in control antiseptic sea water not
Remain effective chlorine composition.
(2) the purebred liquid of egg capsule algae is prepared：
By step 1 choose egg capsule frustule, be seeded in above-mentioned egg capsule algae special culture media, and temperature for 25 DEG C,
Continuous charge culture purified under conditions of illumination 2000Lx when egg capsule concentration of algae reaches ten thousand/ml of 65-75 after 15 days, stops filling
Gas, venting supernatant liquor after precipitation obtain the purebred liquid of egg capsule algae.
Embodiment 2：
A kind of preparation method of the purebred liquid of egg capsule algae, comprises the following steps：
Step 1: the separation that egg capsule algae is purebred
By gathering out egg capsule algae sample in high yield shrimp culture pond, then being separated under microscope and choosing egg capsule frustule；
Step 2: the cultivation of pure oocysts algae
(1) according to the following ratio, egg capsule algae special culture media is prepared：
The proportion of prepared egg capsule algae special culture media is 1.014, pH value 8.0.
Wherein, the preparation method of antiseptic sea water is：By seawater after the filtering of Tao Shi hyperfiltration device, add in effective chlorine and contain
The strong chlorine oil 15ppm for 50% is measured, inflation 2h makes drug fully dissolve, then stops inflation, after precipitating for 24 hours, adds in thio sulphur
In sour sodium 5ppm and remaining chlorine, and the chlorinity in antiseptic sea water is measured with starch potassium iodide liquid, in control antiseptic sea water not
Remain effective chlorine composition.
(2) the purebred liquid of egg capsule algae is prepared：
By step 1 choose egg capsule frustule, be seeded in above-mentioned egg capsule algae special culture media, and temperature for 23 DEG C,
Continuous charge culture purified under conditions of illumination 1800Lx when egg capsule concentration of algae reaches ten thousand/ml of 65-75 after 12 days, stops filling
Gas, venting supernatant liquor after precipitation obtain the purebred liquid of egg capsule algae.
Embodiment 3：
A kind of preparation method of the purebred liquid of egg capsule algae, comprises the following steps：
Step 1: the separation that egg capsule algae is purebred
By gathering out egg capsule algae sample in high yield shrimp culture pond, then being separated under microscope and choosing egg capsule frustule；
Step 2: the cultivation of pure oocysts algae
(1) according to the following ratio, egg capsule algae special culture media is prepared：
The proportion of prepared egg capsule algae special culture media is 1.013, pH value 8.2.
Wherein, the preparation method of antiseptic sea water is：By seawater after the filtering of Tao Shi hyperfiltration device, add in effective chlorine and contain
The strong chlorine oil 25ppm for 50% is measured, inflation 2h makes drug fully dissolve, then stops inflation, after precipitating for 24 hours, adds in thio sulphur
In sour sodium 10ppm and remaining chlorine, and the chlorinity in antiseptic sea water is measured with starch potassium iodide liquid, in control antiseptic sea water not
Remain effective chlorine composition.
(2) the purebred liquid of egg capsule algae is prepared：
By step 1 choose egg capsule frustule, be seeded in above-mentioned egg capsule algae special culture media, and temperature for 28 DEG C,
Continuous charge culture purified under conditions of illumination 1900Lx when egg capsule concentration of algae reaches ten thousand/ml of 65-75 after 18 days, stops filling
Gas, venting supernatant liquor after precipitation obtain the purebred liquid of egg capsule algae.
Embodiment 4：
A kind of preparation method of the purebred liquid of egg capsule algae, comprises the following steps：
Step 1: the separation that egg capsule algae is purebred
By gathering out egg capsule algae sample in high yield shrimp culture pond, then being separated under microscope and choosing egg capsule frustule.
Step 2: the cultivation of pure oocysts algae
(1) according to the following ratio, egg capsule algae special culture media is prepared：
The proportion of prepared egg capsule algae special culture media is 1.010, pH value 8.4.
Wherein, the preparation method of antiseptic sea water is：By seawater after the filtering of Tao Shi hyperfiltration device, add in effective chlorine and contain
The strong chlorine oil 22ppm for 50% is measured, inflation 2h makes drug fully dissolve, then stops inflation, after precipitating for 24 hours, adds in thio sulphur
In sour sodium 9ppm and remaining chlorine, and the chlorinity in antiseptic sea water is measured with starch potassium iodide liquid, in control antiseptic sea water not
Remain effective chlorine composition.
(2) the purebred liquid of egg capsule algae is prepared：
By step 1 choose egg capsule frustule, be seeded in above-mentioned egg capsule algae special culture media, and temperature for 26 DEG C,
Continuous charge culture purified under conditions of illumination 2000Lx when egg capsule concentration of algae reaches ten thousand/ml of 65-75 after 16 days, stops filling
Gas, venting supernatant liquor after precipitation obtain the purebred liquid of egg capsule algae.
Finally it should be noted that above example is merely to illustrate technical scheme rather than the present invention is protected
The limitation of scope, although being explained in detail with reference to preferred embodiment to the present invention, those of ordinary skill in the art should manage
Solution, technical scheme can be modified or replaced equivalently, without departing from technical solution of the present invention essence and
Scope.
Claims (4)
1. a kind of egg capsule algae special culture media, it is characterised in that：It is made of the component of following proportioning：
The proportion of the egg capsule algae special culture media is 1.010~1.016, and pH value is 8.0~8.4.
2. a kind of egg capsule algae special culture media according to claim 1, it is characterised in that：By the component system of following proportioning
Into：
3. a kind of egg capsule algae special culture media according to claim 1 or 2, it is characterised in that：The system of the antiseptic sea water
Preparation Method is：By seawater through Tao Shi hyperfiltration device filtering after, add in available chlorine content be 50% strong chlorine oil 15~
25ppm, inflation 2h makes drug fully dissolve, then stops inflation, after precipitating for 24 hours, adds in 5~10ppm of sodium thiosulfate and neutralizes
Remaining chlorine, and with starch potassium iodide liquid measure antiseptic sea water in chlorinity, control antiseptic sea water in do not remain effective chlorine into
Part.
4. a kind of preparation method of the purebred liquid of egg capsule algae, it is characterised in that：Comprise the following steps：
Step 1: the separation of pure oocysts algae
By gathering out egg capsule algae sample in high yield shrimp culture pond, then being separated under microscope and choosing egg capsule frustule；
Step 2: the cultivation of pure oocysts algae
The egg capsule frustule that step 1 is chosen, is seeded to the egg capsule algae special culture media described in claims 1 to 3 any one
In, temperature be 23~28 DEG C, continuous charge culture purified under conditions of 1800~2000Lx of illumination, egg capsule algae after 12~18 days
When concentration reaches ten thousand/ml of 65-75, stop inflating, venting supernatant liquor after precipitation obtains the purebred liquid of egg capsule algae.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110760446A (en) * | 2019-12-12 | 2020-02-07 | 宁波浮田生物技术有限公司 | Culture process of oocyst algae |
| CN112457995A (en) * | 2020-12-11 | 2021-03-09 | 武汉市中易天地物联科技有限公司 | Special culture medium for producing concentrated oocystis and culture method thereof |
| CN112553081A (en) * | 2020-12-11 | 2021-03-26 | 武汉市中易天地物联科技有限公司 | Method for culturing oocyst algae by heterotrophic-autotrophic two-step method |
Citations (1)
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| CN103820325A (en) * | 2014-03-03 | 2014-05-28 | 临沂大学 | High-density culture technology for oocystis borgei and collection method for oocystis borgei cells |
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2018
- 2018-01-05 CN CN201810011666.5A patent/CN108060086A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820325A (en) * | 2014-03-03 | 2014-05-28 | 临沂大学 | High-density culture technology for oocystis borgei and collection method for oocystis borgei cells |
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| 江山等: "南美白对虾反季节多茬养殖模式", 《致富经典》 * |
| 董龙香等: "波吉卵囊藻对重金属铬的耐受性研究", 《淡水渔业》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110760446A (en) * | 2019-12-12 | 2020-02-07 | 宁波浮田生物技术有限公司 | Culture process of oocyst algae |
| CN112457995A (en) * | 2020-12-11 | 2021-03-09 | 武汉市中易天地物联科技有限公司 | Special culture medium for producing concentrated oocystis and culture method thereof |
| CN112553081A (en) * | 2020-12-11 | 2021-03-26 | 武汉市中易天地物联科技有限公司 | Method for culturing oocyst algae by heterotrophic-autotrophic two-step method |
| CN112553081B (en) * | 2020-12-11 | 2023-01-31 | 武汉市中易天地物联科技有限公司 | Method for culturing oocyst algae by heterotrophic-autotrophic transfer two-step method |
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Application publication date: 20180522 |
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