CN108041101A - Mango sapling method for producing insecticide - Google Patents

Mango sapling method for producing insecticide Download PDF

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Publication number
CN108041101A
CN108041101A CN201711225429.0A CN201711225429A CN108041101A CN 108041101 A CN108041101 A CN 108041101A CN 201711225429 A CN201711225429 A CN 201711225429A CN 108041101 A CN108041101 A CN 108041101A
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parts
mango
sapling
chaste tree
seed
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周建波
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Lanxi Silent Biotechnology Co Ltd
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Lanxi Silent Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/30Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/46Stemonaceae [Stemona family], e.g. croomia

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of mango sapling method for producing insecticide, ingredient and its parts by weight are 5 10 parts of five-leaved chaste tree seed, 8 15 parts of neem seed, 13 parts of synergist, 13 parts of the tuber of stemona, 13 parts of fragrant eupatorium herb, 36 parts of henbane.Synergist is the one or more in 40 60% hemp oils and 40 60% sapns or tween.It has the beneficial effect that:In the present invention there is apparent mutually coordinated effect in each ingredient, it can be done directly on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness death rapidly, can epidemic disease be triggered in population by laterally infection simultaneously, it is infected by longitudinal direction and kills pupa and ovum, effectively control pest population and its harm.The present invention is rapid to the killing speed of pest, and half a day is only needed to the time since the dead worm that sprays to.

Description

Mango sapling method for producing insecticide
Technical field
The present invention relates to field of pesticides, more particularly, to a kind of mango sapling method for producing insecticide.
Background technology
With the development of modern science technology, chemical pesticide is because its adaptation is wide, easy to operate, at low cost, significant effect The advantages that be widely used.But since the desinsection specificity of chemical pesticide is not strong, the field longevity of residure is longer, while easily induce Pest develops immunity to drugs to it, particularly pollution of the chemical residue pesticide to agricultural product and environment, causes women's miscarriage, baby abnormal Shape and the diseases such as various cancers of the mankind are induced, therefore the attention of the mankind is increasingly subject to using biological pesticide pest control.Biology Pesticide mainly includes microbial pesticide, botanical pesticide and animal pesticide etc..It is raw for conventional chemical pesticide Object pesticide has the mode of action unique, prevents that object is single-minded, and to the beneficial organisms such as natural enemy safety, dosage is small, and degradation is fast, to people, Poultry, environmental risks are low, the features such as especially suitable for disease, worm, crop smothering integrated control.The increasing realized with human environment protection By force, the biological pesticide of high-efficiency low-toxicity has become the developing direction of current pesticide.
The content of the invention
One of the objects of the present invention is to provide a kind of quick desinsection of energy, and the desinsection lasting period is long, free from environmental pollution, right Mango fruit tree has growth promoting function, and to safety of human and livestock, long-time service pest does not generate the mango sapling insecticide of resistance easily.
The second object of the present invention is to provide that a kind of preparation process is simple, and raw material sources are wide, the awns of low production cost Fruit tree sapling method for producing insecticide.
The problem of present invention in background technology for mentioning, the technical solution taken is:Mango sapling insecticide, ingredient And its parts by weight for 5-10 parts of five-leaved chaste tree seed, 8-15 parts of neem seed, 1-3 parts of synergist, 1-3 parts of the tuber of stemona, 1-3 parts of fragrant eupatorium herb, 3-6 parts of henbane.Synergist is the one or more in 40-60% hemp oils and 40-60% sapns or tween.
Hemp oil extracting method is:It by the fresh ramie plant shearing of acquisition, crushes, crosses 20-80 mesh sieves, ramie is taken to plant Strain powder adds in acetone, and stirring and leaching 5-9h is centrifuged, and be concentrated under reduced pressure to obtain hemp oil.
Mango sapling method for producing insecticide, comprises the following steps:
1)Pretreatment:Steam blasting is carried out after neem seed/five-leaved chaste tree seed is carried out pre-preg, pressure is controlled in 3.0- 4.0MPa holds time as 40-70s, obtains neem seed explosion object;Petroleum ether extraction is added in, layering takes phase, obtains print chinaberry Seed extract/five-leaved chaste tree seed extract;
2)Fermentation:0.5-0.6g/L peptones 0.7- is added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder 1.6mg/L Beta-alanines are inoculated with cellulase producing strain, in 22-28 DEG C, initial pH5.0-7.0, and 100-200r/min shaking flasks Fermented and cultured 60-84h, high speed centrifugation take zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, and is waited to produce Separation screening and optimization culture conditions [J] Gansu the agricultural university journal of cellulase fungal bacterial strain, 2016,51 (2):8-15. In aspergillus fumigatus bacterial strain B-2-3.Above-mentioned cellulase producing strain generates substantial amounts of cellulase during fermentation, can by the tuber of stemona, Cellulase hydrolysis on fragrant eupatorium herb and henbane cell membrane, active ingredient dissolution.The zymotic fluid pair obtained using above-mentioned fermentation operation The lethality of pest is better than the enzymolysis liquid directly obtained with the cellulase degradation tuber of stemona, fragrant eupatorium herb and henbane, but specific effect Mechanism is still not clear;
3)It is prepared by finished product:Neem seed extract and five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid The 1/6-1/4 of original volume is concentrated into after uniformly, obtains mango sapling insecticide.There are apparent phases for each ingredient in the present invention Mutual coordinative role, the end product properties being prepared are stablized, long shelf-life;After sprinkling, in mango fruit tree leaf wettability of the surface Height, drug effect lasting period are long;It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to contaminate rapidly It dies of illness and dies, while can epidemic disease be triggered in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively controlled Pest population and its harm.The present invention is rapid to the killing speed of pest, when the dead worm that sprays to only needs half a day to one Between.
The pre-preg of neem seed/five-leaved chaste tree seed is:Commercially available neem seed/five-leaved chaste tree seed is taken, removes impurity, is crushed, 15-20 mesh sieves are crossed, add in 15-20 parts of distilled water, stir 30-35min, sealing and standing 6-10h.Pre-preg adds print chinaberry kind The humidity of son/five-leaved chaste tree seed, material are fully swollen, the osmotic efficiency of steam when improving steam treatment, improve demolition effect, are promoted Into the release of nimbin/five-leaved chaste tree total alkaloid, plant resources is made to be fully used.
The addition of petroleum ether is 0.7-1.5 times of the distilled water volume added in during explosion.Nimbin/five-leaved chaste tree total alkaloid For liposoluble substance, and its solubility in petroleum ether is high, uses petroleum ether as extractant, can be significantly while being enriched with Reduce the loss of nimbin.
Compared with prior art, the advantage of the invention is that:In the present invention there is the apparent effect of being coupled, system in each ingredient Standby obtained end product properties are stablized, long shelf-life;It is high in mango fruit tree leaf wettability of the surface after sprinkling, the drug effect lasting period It is long;It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness death rapidly, while can To trigger epidemic disease in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively control pest population and its Harm.The present invention is rapid to the killing speed of pest, and half a day is only needed to the time since the dead worm that sprays to.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
Mango sapling insecticide, ingredient and its parts by weight are 10 parts of five-leaved chaste tree seed, 10 parts of neem seed, 2 parts of synergist, the tuber of stemona 3 parts, 3 parts of fragrant eupatorium herb, 5 parts of henbane.Synergist is 50% hemp oil and 50% Arlacel-20.
Hemp oil extracting method is:It by the fresh ramie plant shearing of acquisition, crushes, crosses 50 mesh sieves, take ramie plant Powder adds in the acetone of 5 times of volumes, and stirring and leaching 7h is centrifuged, and be concentrated under reduced pressure to obtain hemp oil.
Mango sapling method for producing insecticide, comprises the following steps:
1)Pretreatment:Steam blasting is carried out after neem seed/five-leaved chaste tree seed is carried out pre-preg, pressure is controlled in 3.5MPa, It holds time as 50s, obtains neem seed explosion object/five-leaved chaste tree seed explosion object;Petroleum ether extraction is added in, layering takes phase, obtains To neem seed extract/five-leaved chaste tree seed extract;
2)Fermentation:Added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder 0.55g/L peptones and 1mg/L β- Alanine is inoculated with cellulase producing strain, in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, high speed centrifugation Take zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits the separation of cellulase-producing fungi bacterial strains Screening and optimization culture conditions [J] Gansu agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2-3 in 8-15.;
3)It is prepared by finished product:Neem seed extract, five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid The 1/5 of original volume is concentrated into after uniformly, obtains mango sapling insecticide.In the present invention there is apparent mutually association in each ingredient Tune acts on, and the end product properties being prepared are stablized, long shelf-life;High in mango fruit tree wettability of the surface after sprinkling, drug effect is held The effect phase is long;It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness death rapidly, together When can epidemic disease be triggered in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively control pest population And its harm.The present invention is rapid to the killing speed of pest, and half a day is only needed to the time since the dead worm that sprays to.
The pre-preg of neem seed/five-leaved chaste tree seed is:Commercially available neem seed/five-leaved chaste tree seed is taken, removes impurity, is crushed, 20 mesh sieves are crossed, add in 15 parts of distilled water, stir 30min, sealing and standing 8h.
The addition of petroleum ether is 1.2 times of the distilled water volume added in during explosion.
Embodiment 2:
Mango sapling insecticide, ingredient and its parts by weight are 8 parts of five-leaved chaste tree seed, 12 parts of neem seed, 2 parts of synergist, the tuber of stemona 2 Part, 3 parts of fragrant eupatorium herb, 6 parts of henbane.Synergist is 50% hemp oil and 50% Arlacel-20 and Tween-80.
Hemp oil extracting method is:It by the fresh ramie plant shearing of acquisition, crushes, crosses 50 mesh sieves, take ramie plant Powder adds in the acetone of 5 times of volumes, and stirring and leaching 7h is centrifuged, and be concentrated under reduced pressure to obtain hemp oil.
Mango sapling method for producing insecticide, comprises the following steps:
1)Pretreatment:Steam blasting is carried out after neem seed/five-leaved chaste tree seed is carried out pre-preg, pressure is controlled in 3.5MPa, It holds time as 50s, obtains neem seed explosion object/five-leaved chaste tree seed explosion object;Petroleum ether extraction is added in, layering takes phase, obtains To neem seed extract/five-leaved chaste tree seed extract;
2)Fermentation:Added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder 0.58g/L peptone 1.2mg/L β- Alanine is inoculated with cellulase producing strain, in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, high speed centrifugation Take zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits the separation of cellulase-producing fungi bacterial strains Screening and optimization culture conditions [J] Gansu agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2-3 in 8-15.;
3)It is prepared by finished product:Neem seed extract, five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid The 1/6 of original volume is concentrated into after uniformly, obtains mango sapling insecticide.In the present invention there is apparent mutually association in each ingredient Tune acts on, and the end product properties being prepared are stablized, long shelf-life;High in mango fruit tree wettability of the surface after sprinkling, drug effect is held The effect phase is long;It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness death rapidly, together When can epidemic disease be triggered in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively control pest population And its harm.The present invention is rapid to the killing speed of pest, and half a day is only needed to the time since the dead worm that sprays to.
The pre-preg of neem seed/five-leaved chaste tree seed is:Commercially available neem seed/five-leaved chaste tree seed is taken, removes impurity, is crushed, 20 mesh sieves are crossed, add in 20 parts of distilled water, stir 30min, sealing and standing 8h.
The addition of petroleum ether is 1.2 times of the distilled water volume added in during explosion.
Embodiment 3:
Mango sapling insecticide, ingredient and its parts by weight are 5 parts of five-leaved chaste tree seed, 10 parts of neem seed, 2 parts of synergist, the tuber of stemona 3 Part, 3 parts of fragrant eupatorium herb, 6 parts of henbane.Synergist is 40% hemp oil and 60% Tween-80.
Hemp oil extracting method is:It by the fresh ramie plant shearing of acquisition, crushes, crosses 50 mesh sieves, take ramie plant Powder adds in the acetone of 5 times of volumes, and stirring and leaching 7h is centrifuged, and be concentrated under reduced pressure to obtain hemp oil.
Mango sapling method for producing insecticide, Optimization Steps:
1)Pretreatment:Steam blasting is carried out after neem seed/five-leaved chaste tree seed is carried out pre-preg, pressure is controlled in 3.5MPa, It holds time as 50s, obtains neem seed explosion object/five-leaved chaste tree seed explosion object;Petroleum ether solution extraction is added in, layering takes Phase obtains neem seed extract/five-leaved chaste tree seed extract.Wherein petroleum ether solution is the 4- phenyl dissolved with 0.1% in petroleum ether Butyric acid, compared to pure petroleum ether, petroleum ether solution of the invention is more to the extraction quantity of nimbin;
2)Fermentation:0.6g/L peptones 1.2mg/L β-ammonia is added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder Base propionic acid is inoculated with cellulase producing strain, and in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, high speed centrifugation takes Zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits the bolter of cellulase-producing fungi bacterial strains Choosing and optimization culture conditions [J] Gansu agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2-3 in 8-15.;
3)It is prepared by finished product:Neem seed extract, five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid The 1/6 of original volume is concentrated into after uniformly, obtains mango sapling insecticide.In the present invention there is apparent mutually association in each ingredient Tune acts on, and the end product properties being prepared are stablized, long shelf-life;It is high in mango fruit tree leaf wettability of the surface after sprinkling, medicine It is long to imitate the lasting period;It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness extremely rapidly It dies, while can epidemic disease be triggered in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively control pest Population and its harm.The present invention is rapid to the killing speed of pest, and half a day is only needed to the time since the dead worm that sprays to.
The pre-preg of neem seed/five-leaved chaste tree seed is:Commercially available neem seed/five-leaved chaste tree seed is taken, removes impurity, is crushed, 20 mesh sieves are crossed, add in 18 parts of distilled water, stir 30min, sealing and standing 8h.
The addition of petroleum ether is 1.3 times of the distilled water volume added in during explosion.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that the above is only For specific embodiments of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.

Claims (7)

1. mango sapling insecticide, it is characterised in that ingredient and its parts by weight are 5-10 parts of five-leaved chaste tree seed, neem seed 8-15 Part, 1-3 parts of synergist, 1-3 parts of the tuber of stemona, 1-3 parts of fragrant eupatorium herb, 3-6 parts of henbane.
2. mango sapling insecticide according to claim 1, it is characterised in that:The synergist is 40-60% ramies One or more in oil and 40-60% sapns or tween.
3. mango sapling insecticide according to claim 2, it is characterised in that:The hemp oil extracting method is: It by the fresh ramie plant shearing of acquisition, crushes, crosses 20-80 mesh sieves, take ramie plant powder, add in acetone, stirring and leaching 5-9h is centrifuged, and be concentrated under reduced pressure to obtain hemp oil.
4. mango sapling method for producing insecticide, comprises the following steps:
1)Pretreatment:Steam blasting is carried out after neem seed and five-leaved chaste tree seed are carried out pre-preg respectively, pressure control exists 3.0-4.0MPa holds time as 40-70s, respectively obtains neem seed explosion object and five-leaved chaste tree seed explosion object;Add in petroleum ether Extraction, layering take phase, respectively obtain neem seed extract and five-leaved chaste tree seed extract;
2)Fermentation:0.5-0.6g/L peptones and 0.7- are added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder 1.6mg/L Beta-alanines, are inoculated with cellulase producing strain, shake flask fermentation culture 60-84h, and high speed centrifugation takes zymotic fluid;
3)It is prepared by finished product:Neem seed extract, five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid The 1/6-1/4 of original volume is concentrated into after uniformly, obtains mango sapling insecticide.
5. mango sapling method for producing insecticide according to claim 4, it is characterised in that:The print chinaberry kind The pre-preg of son/five-leaved chaste tree seed is:Commercially available neem seed/five-leaved chaste tree seed is taken, removes impurity, is crushed, 15-20 mesh sieves is crossed, adds Enter 15-20 parts of distilled water, stir 30-35min, sealing and standing 6-10h.
6. mango sapling method for producing insecticide according to claim 4, it is characterised in that:The petroleum ether Addition is 0.7-1.5 times of the distilled water volume added in during explosion.
7. mango sapling method for producing insecticide according to claim 4, it is characterised in that:The shake flask fermentation Condition of culture is:22-28 DEG C of fermentation temperature, initial pH5.0-7.0, shaking flask rotary speed 100-200r/min.
CN201711225429.0A 2017-11-29 2017-11-29 Mango sapling method for producing insecticide Pending CN108041101A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108450491A (en) * 2018-01-19 2018-08-28 磐安海璞食品科技有限公司 A kind of rice high efficient insecticide

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102696693A (en) * 2012-05-15 2012-10-03 贵阳学院 Compound type botanical pesticide and manufacturing method and application thereof
CN104322572A (en) * 2014-10-24 2015-02-04 河南省科高植物天然产物开发工程技术有限公司 Botanical insecticide
WO2015160618A1 (en) * 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and a biological control agent
CN107006533A (en) * 2017-05-05 2017-08-04 安徽帝都农业生态园有限公司 One planting fruit-trees efficient pesticides and its preparation method and application
CN107279196A (en) * 2017-06-27 2017-10-24 浦江县元寿农业科技有限公司 Plant source environment-friendly pesticide

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102696693A (en) * 2012-05-15 2012-10-03 贵阳学院 Compound type botanical pesticide and manufacturing method and application thereof
WO2015160618A1 (en) * 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and a biological control agent
CN104322572A (en) * 2014-10-24 2015-02-04 河南省科高植物天然产物开发工程技术有限公司 Botanical insecticide
CN107006533A (en) * 2017-05-05 2017-08-04 安徽帝都农业生态园有限公司 One planting fruit-trees efficient pesticides and its preparation method and application
CN107279196A (en) * 2017-06-27 2017-10-24 浦江县元寿农业科技有限公司 Plant source environment-friendly pesticide

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108450491A (en) * 2018-01-19 2018-08-28 磐安海璞食品科技有限公司 A kind of rice high efficient insecticide

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Application publication date: 20180518