CN108450491A - A kind of rice high efficient insecticide - Google Patents
A kind of rice high efficient insecticide Download PDFInfo
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- CN108450491A CN108450491A CN201810053932.0A CN201810053932A CN108450491A CN 108450491 A CN108450491 A CN 108450491A CN 201810053932 A CN201810053932 A CN 201810053932A CN 108450491 A CN108450491 A CN 108450491A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of rice high efficient insecticide, ingredient and its parts by weight are 5 10 parts of five-leaved chaste tree seed, 8 15 parts of neem seed, 13 parts of synergist, 13 parts of the tuber of stemona, 13 parts of fragrant eupatorium herb, 36 parts of henbane.Synergist is one or more in 40 60% hemp oils and 40 60% sapns or tween.It has the beneficial effect that：In the present invention there is apparent mutually coordinated effect in each ingredient, it can be done directly on the fat-body and midgut epithelial cells core of insect larvae, and replicate causes larva to catch an illness death rapidly, it can cause epidemic disease in population by laterally infection simultaneously, pupa and ovum are killed by longitudinal infect, effectively controls pest population and its harm.The present invention is rapid to the killing speed of pest, only needs half a day to the time since the dead worm that sprays to.
Description
Technical field
The present invention relates to field of pesticides, more particularly, to a kind of rice high efficient insecticide.
Background technology
With the development of modern science technology, chemical pesticide is because its adaptation is wide, easy to operate, at low cost, significant effect
The advantages that be widely used.But since the desinsection specificity of chemical pesticide is not strong, the field longevity of residure is longer, while being easy to induce
Pest develops immunity to drugs to it, especially pollution of the chemical residue pesticide to agricultural product and environment, causes women's miscarriage, baby abnormal
Shape and the diseases such as various cancers of the mankind are induced, therefore is increasingly subject to the attention of the mankind using biological pesticide pest control.Biology
Pesticide includes mainly microbial pesticide, botanical pesticide and animal pesticide etc..It is raw for conventional chemical pesticide
Object pesticide has the mode of action unique, prevents object single-minded, and to the beneficial organisms such as natural enemy safety, dosage is small, and degradation is fast, to people,
Poultry, environmental risks are low, the features such as especially suitable for disease, worm, crop smothering integrated control.The increasing realized with human environment protection
By force, the biological pesticide of high-efficiency low-toxicity has become the developing direction of current pesticide.
Invention content
The purpose of the present invention is to provide a kind of quick desinsections of energy, and the desinsection lasting period is long, free from environmental pollution, to mango
Fruit tree has growth promoting function, and to safety of human and livestock, a kind of rice high efficient insecticide that pest does not generate resistance easily is used for a long time.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is：A kind of rice high efficient insecticide, at
Divide and its parts by weight are 5-10 parts of five-leaved chaste tree seed, 8-15 parts of neem seed, 1-3 parts of synergist, 1-3 parts of the tuber of stemona, fragrant eupatorium herb 1-3
Part, 3-6 parts of henbane.Synergist is one or more in 40-60% hemp oils and 40-60% sapns or tween.
Hemp oil extracting method is：It by the fresh ramie plant shearing of acquisition, crushes, crosses 20-80 mesh sieve, ramie is taken to plant
Strain powder, is added acetone, and stirring and leaching 5-9h centrifuges, hemp oil is concentrated under reduced pressure to obtain.
A kind of preparation method of rice high efficient insecticide, includes the following steps：
1）Pretreatment：Steam blasting is carried out after neem seed/five-leaved chaste tree seed is carried out pre-preg, pressure is controlled in 3.0-
4.0MPa holds time as 40-70s, obtains neem seed explosion object；Petroleum ether extraction is added, layering takes phase, obtains print chinaberry
Seed extract/five-leaved chaste tree seed extract；
2）Fermentation：0.5-0.6g/L peptones 0.7- is added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder
1.6mg/L Beta-alanines are inoculated with cellulase producing strain, in 22-28 DEG C, initial pH5.0-7.0, and 100-200r/min shaking flasks
Fermented and cultured 60-84h, high speed centrifugation take zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, and is waited to produce
The Gansu separation screening and optimization culture conditions [J] the agricultural university journal of cellulase fungal bacterial strain, 2016,51 (2):8-15.
In aspergillus fumigatus bacterial strain B-2-3.Above-mentioned cellulase producing strain generates a large amount of cellulase during fermentation, can by the tuber of stemona,
Cellulase hydrolysis on fragrant eupatorium herb and henbane cell wall, active ingredient dissolution.The zymotic fluid pair obtained using above-mentioned fermentation operation
The lethality of pest is better than the enzymolysis liquid directly obtained with the cellulase degradation tuber of stemona, fragrant eupatorium herb and henbane, but specific effect
Mechanism is still not clear；
3）It is prepared by finished product：Neem seed extract and five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid
It is concentrated into the 1/6-1/4 of original volume after uniformly, obtains a kind of rice high efficient insecticide.Each ingredient exists apparent in the present invention
Mutually coordinated effect, the end product properties being prepared are stablized, long shelf-life；After sprinkling, the wetting on mango fruit tree leaf surface
Property it is high, the drug effect lasting period is long；It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and duplication leads to larva rapidly
It catches an illness death, while epidemic disease can be caused in population by laterally infection, pass through longitudinal infect and kill pupa and ovum, effectively control
Pest population processed and its harm.The present invention is rapid to the killing speed of pest, only needs half a day by one day since the dead worm that sprays to
Time.
The pre-preg of neem seed/five-leaved chaste tree seed is：Commercially available neem seed/five-leaved chaste tree seed is taken, impurity is removed, is crushed,
15-20 mesh sieve is crossed, 15-20 parts of distilled water are added, stirs 30-35min, sealing and standing 6-10h.Pre-preg increases print chinaberry kind
The humidity of son/five-leaved chaste tree seed, material are fully swollen, the osmotic efficiency of steam when improving steam treatment, improve demolition effect, are promoted
Into the release of nimbin/five-leaved chaste tree total alkaloid, plant resources is made to be fully used.
The addition of petroleum ether is 0.7-1.5 times of the distilled water volume being added when explosion.Nimbin/five-leaved chaste tree total alkaloid
For liposoluble substance, and its solubility in petroleum ether is high, uses petroleum ether for extractant, can be significantly while being enriched with
Reduce the loss of nimbin.
Compared with the prior art, the advantages of the present invention are as follows：In the present invention there is the apparent effect of being coupled, system in each ingredient
Standby obtained end product properties are stablized, long shelf-life；It is high in mango fruit tree leaf wettability of the surface after sprinkling, the drug effect lasting period
It is long；It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness death rapidly, while can
To cause epidemic disease in population by laterally infection, pupa and ovum are killed by longitudinal infect, effectively control pest population and its
Harm.The present invention is rapid to the killing speed of pest, only needs half a day to the time since the dead worm that sprays to.
Specific implementation mode
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of rice high efficient insecticide, ingredient and its parts by weight are 10 parts of five-leaved chaste tree seed, 10 parts of neem seed, 2 parts of synergist, hundred
3 parts of portion, 3 parts of fragrant eupatorium herb, 5 parts of henbane.Synergist is 50% hemp oil and 50% Arlacel-20.
Hemp oil extracting method is：It by the fresh ramie plant shearing of acquisition, crushes, crosses 50 mesh sieve, take ramie plant
Powder, is added the acetone of 5 times of volumes, and stirring and leaching 7h centrifuges, hemp oil is concentrated under reduced pressure to obtain.
A kind of preparation method of rice high efficient insecticide, includes the following steps：
1）Pretreatment：Steam blasting is carried out after neem seed/five-leaved chaste tree seed is carried out pre-preg, pressure is controlled in 3.5MPa,
It holds time as 50s, obtains neem seed explosion object/five-leaved chaste tree seed explosion object；Petroleum ether extraction is added, layering takes phase, obtains
To neem seed extract/five-leaved chaste tree seed extract；
2）Fermentation：0.55g/L peptones and 1mg/L β-are added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder
Alanine is inoculated with cellulase producing strain, in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, high speed centrifugation
Take zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits the separation of cellulase-producing fungi bacterial strains
Screening and the Gansu optimization culture conditions [J] agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2-3 in 8-15.；
3）It is prepared by finished product：Neem seed extract, five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid
It is concentrated into the 1/5 of original volume after uniformly, obtains a kind of rice high efficient insecticide.Each ingredient exists apparent mutual in the present invention
Coordinative role, the end product properties being prepared are stablized, long shelf-life；It is high in mango fruit tree wettability of the surface after sprinkling, drug effect
Lasting period is long；It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness death rapidly,
It can cause epidemic disease in population by laterally infection simultaneously, pupa and ovum are killed by longitudinal infect, effectively control species of insect pests
Group and its harm.The present invention is rapid to the killing speed of pest, only needs half a day to the time since the dead worm that sprays to.
The pre-preg of neem seed/five-leaved chaste tree seed is：Commercially available neem seed/five-leaved chaste tree seed is taken, impurity is removed, is crushed,
20 mesh sieve is crossed, 15 parts of distilled water are added, stirs 30min, sealing and standing 8h.
The addition of petroleum ether is 1.2 times of the distilled water volume being added when explosion.
Embodiment 2：
A kind of rice high efficient insecticide, ingredient and its parts by weight are 8 parts of five-leaved chaste tree seed, 12 parts of neem seed, 2 parts of synergist, hundred
2 parts of portion, 3 parts of fragrant eupatorium herb, 6 parts of henbane.Synergist is 50% hemp oil and 50% Arlacel-20 and Tween-80.
Hemp oil extracting method is：It by the fresh ramie plant shearing of acquisition, crushes, crosses 50 mesh sieve, take ramie plant
Powder, is added the acetone of 5 times of volumes, and stirring and leaching 7h centrifuges, hemp oil is concentrated under reduced pressure to obtain.
A kind of preparation method of rice high efficient insecticide, includes the following steps：
1）Pretreatment：Steam blasting is carried out after neem seed/five-leaved chaste tree seed is carried out pre-preg, pressure is controlled in 3.5MPa,
It holds time as 50s, obtains neem seed explosion object/five-leaved chaste tree seed explosion object；Petroleum ether extraction is added, layering takes phase, obtains
To neem seed extract/five-leaved chaste tree seed extract；
2）Fermentation：0.58g/L peptone 1.2mg/L β-are added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder
Alanine is inoculated with cellulase producing strain, in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, high speed centrifugation
Take zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits the separation of cellulase-producing fungi bacterial strains
Screening and the Gansu optimization culture conditions [J] agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2-3 in 8-15.；
3）It is prepared by finished product：Neem seed extract, five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid
It is concentrated into the 1/6 of original volume after uniformly, obtains a kind of rice high efficient insecticide.Each ingredient exists apparent mutual in the present invention
Coordinative role, the end product properties being prepared are stablized, long shelf-life；It is high in mango fruit tree wettability of the surface after sprinkling, drug effect
Lasting period is long；It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness death rapidly,
It can cause epidemic disease in population by laterally infection simultaneously, pupa and ovum are killed by longitudinal infect, effectively control species of insect pests
Group and its harm.The present invention is rapid to the killing speed of pest, only needs half a day to the time since the dead worm that sprays to.
The pre-preg of neem seed/five-leaved chaste tree seed is：Commercially available neem seed/five-leaved chaste tree seed is taken, impurity is removed, is crushed,
20 mesh sieve is crossed, 20 parts of distilled water are added, stirs 30min, sealing and standing 8h.
The addition of petroleum ether is 1.2 times of the distilled water volume being added when explosion.
Embodiment 3：
A kind of rice high efficient insecticide, ingredient and its parts by weight are 5 parts of five-leaved chaste tree seed, 10 parts of neem seed, 2 parts of synergist, hundred
3 parts of portion, 3 parts of fragrant eupatorium herb, 6 parts of henbane.Synergist is 40% hemp oil and 60% Tween-80.
Hemp oil extracting method is：It by the fresh ramie plant shearing of acquisition, crushes, crosses 50 mesh sieve, take ramie plant
Powder, is added the acetone of 5 times of volumes, and stirring and leaching 7h centrifuges, hemp oil is concentrated under reduced pressure to obtain.
A kind of preparation method of rice high efficient insecticide, Optimization Steps：
1）Pretreatment：Steam blasting is carried out after neem seed/five-leaved chaste tree seed is carried out pre-preg, pressure is controlled in 3.5MPa,
It holds time as 50s, obtains neem seed explosion object/five-leaved chaste tree seed explosion object；Petroleum ether solution extraction is added, layering takes
Phase obtains neem seed extract/five-leaved chaste tree seed extract.Wherein petroleum ether solution is the 4- phenyl dissolved with 0.1% in petroleum ether
Butyric acid, compared to pure petroleum ether, petroleum ether solution of the invention is more to the extraction quantity of nimbin；
2）Fermentation：0.6g/L peptones 1.2mg/L β-ammonia is added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder
Base propionic acid is inoculated with cellulase producing strain, and in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, high speed centrifugation takes
Zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits the bolter of cellulase-producing fungi bacterial strains
Choosing and the Gansu optimization culture conditions [J] agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2-3 in 8-15.；
3）It is prepared by finished product：Neem seed extract, five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid
It is concentrated into the 1/6 of original volume after uniformly, obtains a kind of rice high efficient insecticide.Each ingredient exists apparent mutual in the present invention
Coordinative role, the end product properties being prepared are stablized, long shelf-life；It is high in mango fruit tree leaf wettability of the surface after sprinkling,
The drug effect lasting period is long；It directly acts on the fat-body of insect larvae and midgut epithelial cells core, and replicate causes larva to catch an illness rapidly
Death, while epidemic disease can be caused in population by laterally infection, pupa and ovum are killed by longitudinal infect, effectively control evil
Worm population and its harm.The present invention is rapid to the killing speed of pest, only needs half a day to the time since the dead worm that sprays to.
The pre-preg of neem seed/five-leaved chaste tree seed is：Commercially available neem seed/five-leaved chaste tree seed is taken, impurity is removed, is crushed,
20 mesh sieve is crossed, 18 parts of distilled water are added, stirs 30min, sealing and standing 8h.
The addition of petroleum ether is 1.3 times of the distilled water volume being added when explosion.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of rice high efficient insecticide, it is characterised in that ingredient and its parts by weight are 5-10 parts of five-leaved chaste tree seed, neem seed 8-
15 parts, 1-3 parts of synergist, 1-3 parts of the tuber of stemona, 1-3 parts of fragrant eupatorium herb, 3-6 parts of henbane.
2. a kind of rice high efficient insecticide according to claim 1, it is characterised in that：The synergist is 40-60% ramies
It is one or more in sesame oil and 40-60% sapns or tween.
3. a kind of rice high efficient insecticide according to claim 2, it is characterised in that：The hemp oil extracting method
For：It by the fresh ramie plant shearing of acquisition, crushes, crosses 20-80 mesh sieve, take ramie plant powder, acetone, stirring leaching is added
5-9h is carried, centrifuges, hemp oil is concentrated under reduced pressure to obtain.
4. a kind of rice high efficient insecticide according to claim 1, it is characterised in that：The rice high efficient insecticide
Preparation method includes the following steps：
1）Pretreatment：Steam blasting is carried out after neem seed and five-leaved chaste tree seed are carried out pre-preg respectively, pressure control exists
3.0-4.0MPa holds time as 40-70s, respectively obtains neem seed explosion object and five-leaved chaste tree seed explosion object；Petroleum ether is added
Extraction, layering take phase, respectively obtain neem seed extract and five-leaved chaste tree seed extract；
2）Fermentation：0.5-0.6g/L peptones and 0.7- are added in formula ratio Stemona Root, fragrant eupatorium herb powder and henbane powder
1.6mg/L Beta-alanines, are inoculated with cellulase producing strain, shake flask fermentation culture 60-84h, and high speed centrifugation takes zymotic fluid；
3）It is prepared by finished product：Neem seed extract, five-leaved chaste tree seed extract, formula ratio synergist, stirring are added in zymotic fluid
It is concentrated into the 1/6-1/4 of original volume after uniformly, obtains a kind of rice high efficient insecticide.
5. a kind of rice high efficient insecticide according to claim 4, it is characterised in that：The neem seed/five-leaved chaste tree kind
Son pre-preg be：Commercially available neem seed/five-leaved chaste tree seed is taken, impurity is removed, is crushed, 15-20 mesh sieve is crossed, is added 15-20 parts
Distilled water stirs 30-35min, sealing and standing 6-10h.
6. a kind of rice high efficient insecticide according to claim 4, it is characterised in that：The addition of the petroleum ether is
0.7-1.5 times of the distilled water volume being added when explosion.
7. a kind of rice high efficient insecticide according to claim 4, it is characterised in that：The shake flask fermentation condition of culture
For：22-28 DEG C of fermentation temperature, initial pH5.0-7.0, shaking flask rotary speed 100-200r/min.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810053932.0A CN108450491A (en) | 2018-01-19 | 2018-01-19 | A kind of rice high efficient insecticide |
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|---|---|---|---|
| CN201810053932.0A CN108450491A (en) | 2018-01-19 | 2018-01-19 | A kind of rice high efficient insecticide |
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| CN108450491A true CN108450491A (en) | 2018-08-28 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107279196A (en) * | 2017-06-27 | 2017-10-24 | 浦江县元寿农业科技有限公司 | Plant source environment-friendly pesticide |
| CN108041101A (en) * | 2017-11-29 | 2018-05-18 | 兰溪市沉默生物科技有限公司 | Mango sapling method for producing insecticide |
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2018
- 2018-01-19 CN CN201810053932.0A patent/CN108450491A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107279196A (en) * | 2017-06-27 | 2017-10-24 | 浦江县元寿农业科技有限公司 | Plant source environment-friendly pesticide |
| CN108041101A (en) * | 2017-11-29 | 2018-05-18 | 兰溪市沉默生物科技有限公司 | Mango sapling method for producing insecticide |
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