CN107810994A - Insecticide prepared by microbial fermentation - Google Patents
Insecticide prepared by microbial fermentation Download PDFInfo
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- CN107810994A CN107810994A CN201711018451.8A CN201711018451A CN107810994A CN 107810994 A CN107810994 A CN 107810994A CN 201711018451 A CN201711018451 A CN 201711018451A CN 107810994 A CN107810994 A CN 107810994A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses insecticide prepared by a kind of microbial fermentation, composition and its parts by weight are 8 ~ 15 parts of bacillus thruingensis powder, 8 ~ 15 parts of neem seed, 1 ~ 3 part of synergist, 1 ~ 3 part of the tuber of stemona, 1 ~ 3 part of fragrant eupatorium herb, 3 ~ 6 parts of Eupatorium adenophorum.Synergist is 40 ~ 60% hemp oils and 40 ~ 60% organic silicon surfactants.Have the beneficial effect that：In the present invention there is obvious mutually coordinated effect in each composition, it can be done directly on the fat-body and midgut epithelial cellses core of insect larvae, and replicate causes larva to catch an illness death rapidly, epidemic disease can be triggered in population by laterally infection simultaneously, infected by longitudinal direction and kill pupa and ovum, effectively control pest population and its harm.Different from the insecticide of microbial source, killing speed of the present invention to insect is rapid, and half a day is only needed to the time since the dead worm that sprays to.
Description
Technical field
The present invention relates to field of pesticides, the insecticide prepared more particularly, to a kind of microbial fermentation.
Background technology
With the development of modern science technology, chemical pesticide is because its adaptation is wide, and simple to operate, cost is low, significant effect
The advantages that be widely used.But because the desinsection selectivity of chemical pesticide is not strong, the field longevity of residure is longer, while easily induce
Insect develops immunity to drugs to it, particularly pollution of the chemical residue agricultural chemicals to agricultural product and environment, causes women's miscarriage, baby abnormal
Shape and the disease such as various cancers of the mankind is induced, therefore the attention of the mankind is increasingly subject to using biological pesticide pest control.Biology
Agricultural chemicals mainly includes microbial pesticide, botanical pesticide and animal pesticide etc..It is raw for the chemical pesticide of routine
Thing agricultural chemicals has the mode of action unique, prevents that object is single-minded, and to the beneficial organisms such as natural enemy safety, dosage is small, and degraded is fast, to people,
Poultry, environmental risks are low, the features such as especially suitable for disease, worm, crop smothering integrated control.The increasing realized with human environment protection
By force, the biological pesticide of high-efficiency low-toxicity has turned into the developing direction of current agricultural chemicals.
The content of the invention
It is an object of the invention to provide it is a kind of can quick desinsection, and the desinsection lasting period grow, it is free from environmental pollution, to crop
There is growth promoting function, to person poultry safety, long-term use of insect is not likely to produce insecticide prepared by the microbial fermentation of resistance.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Desinsection prepared by microbial fermentation
Agent, composition and its parts by weight are 8 ~ 15 parts of bacillus thruingensis powder, 8 ~ 15 parts of neem seed, 1 ~ 3 part of synergist, 1 ~ 3 part of the tuber of stemona, fragrant eupatorium herb
1 ~ 3 part, 3 ~ 6 parts of Eupatorium adenophorum.Synergist is 40 ~ 60% hemp oils and 40 ~ 60% organic silicon surfactants.
Hemp oil extracting method is：By the fresh ramie plant shearing of collection, crush, cross 20-80 mesh sieves, take ramie to plant
Strain powder, acetone is added, stirring and leaching 5-9h, is centrifuged, be concentrated under reduced pressure to obtain hemp oil.
The method of insecticide prepared by microbial fermentation, comprises the following steps：
1）Pretreatment：Steam blasting is carried out after neem seed is carried out into pre-preg, Stress control is in 3.0 ~ 4.0MPa, maintenance
Between be 40 ~ 70s, obtain neem seed explosion thing；Petroleum ether extraction is added, layering takes phase, obtains neem seed extract；
2）Fermentation：0.5 ~ 0.6g/L peptones 0.7 are added in formula ratio Stemona Root, fragrant eupatorium herb powder and Eupatorium adenophorum powder
~ 1.6mg/L Beta-alanines, cellulase producing strain is inoculated with, in 22 ~ 28 DEG C, initial pH5.0 ~ 7.0,100 ~ 200r/min shake
Bottle 60 ~ 84h of fermented and cultured, high speed centrifugation take zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits
Separation screening and optimization culture conditions [J] Gansu the agricultural university journal of cellulase-producing fungi bacterial strain, 2016,51 (2):8-
Aspergillus fumigatus bacterial strain B-2-3 in 15..Above-mentioned cellulase producing strain produces substantial amounts of cellulase during fermentation, can be by hundred
Cellulase hydrolysis on portion, fragrant eupatorium herb and Eupatorium adenophorum cell membrane, active ingredient dissolution.The hair obtained using above-mentioned fermentation operation
Zymotic fluid is better than the enzymolysis liquid directly obtained with the cellulase degradation tuber of stemona, fragrant eupatorium herb and Eupatorium adenophorum to the lethality of insect,
But the specific mechanism of action is still not clear；
3）It is prepared by finished product：Neem seed extract, formula ratio bacillus thruingensis powder and synergist are added in zymotic fluid, after stirring
The 1/6 ~ 1/4 of original volume is concentrated into, obtains the insecticide of microbial fermentation preparation.In the present invention there is obvious phase in each composition
Mutual coordinative role, the end product properties being prepared are stable, long shelf-life；After sprinkling, high in the wetability of crop surface, drug effect is held
The effect phase is grown；It directly acts on the fat-body of insect larvae and midgut epithelial cellses core, and replicate causes larva to catch an illness death rapidly, together
When epidemic disease can be triggered in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively control pest population
And its harm.Different from the insecticide of microbial source, killing speed of the present invention to insect is rapid, since the dead worm that sprays to
Half a day is needed to the time.
Neem seed obtains pre-preg：Commercially available neem seed is taken, goes the removal of impurity, is crushed, crosses 15-20 mesh sieves, adds 15-
20 parts of distilled water, stir 30-35min, sealing and standing 6-10h.Pre-preg adds the humidity of neem seed, and material is fully molten
It is swollen, the osmotic efficiency of steam when improving steam treatment, demolition effect is improved, the release of nimbin is promoted, makes plant resources
It is fully used.
The addition of petroleum ether is 0.7 ~ 1.5 times of the distilled water volume added during explosion.Nimbin is liposoluble substance,
And its solubility in petroleum ether is high, uses petroleum ether as extractant, while enrichment, the damage of nimbin can be greatly reduced
Lose.
Compared with prior art, the advantage of the invention is that：In the present invention there is obvious mutually coordinated effect in each composition,
The end product properties being prepared are stable, long shelf-life；It is high in the wetability of crop surface after sprinkling, drug effect lasting period length；Its is straight
Connect the fat-body for acting on insect larvae and midgut epithelial cellses core, and replicate causes larva to catch an illness death rapidly, while can pass through
Laterally infect and trigger epidemic disease in population, infected by longitudinal direction and kill pupa and ovum, effectively control pest population and its harm.No
The insecticide of microbial source is same as, killing speed of the present invention to insect is rapid, and half a day is only needed to one since the dead worm that sprays to
Its time.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
Insecticide prepared by microbial fermentation, composition and its parts by weight are 10 parts of bacillus thruingensis powder, 10 parts of neem seed, synergist 2
Part, 3 parts of the tuber of stemona, 3 parts of fragrant eupatorium herb, 5 parts of Eupatorium adenophorum.Synergist is 50% hemp oil and 50% organic silicon surfactant.
Hemp oil extracting method is：By the fresh ramie plant shearing of collection, crush, cross 50 mesh sieves, take ramie plant
Powder, the acetone of 5 times of volumes is added, stirring and leaching 7h, is centrifuged, be concentrated under reduced pressure to obtain hemp oil.
The method of insecticide prepared by microbial fermentation, comprises the following steps：
1）Pretreatment：Will neem seed carry out pre-preg after carry out steam blasting, Stress control in 3.5MPa, hold time for
50s, obtain neem seed explosion thing；Petroleum ether extraction is added, layering takes phase, obtains neem seed extract；
2）Fermentation：0.55g/L peptones 1mg/L is added in formula ratio Stemona Root, fragrant eupatorium herb powder and Eupatorium adenophorum powder
Beta-alanine, be inoculated with cellulase producing strain, in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, at a high speed from
The heart takes zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits point of cellulase-producing fungi bacterial strains
From screening and optimization culture conditions [J] Gansu agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2-3 in 8-15.；
3）It is prepared by finished product：Neem seed extract, formula ratio bacillus thruingensis powder and synergist are added in zymotic fluid, after stirring
The 1/5 of original volume is concentrated into, obtains the insecticide of microbial fermentation preparation.In the present invention there is obvious mutually association in each composition
Tune acts on, and the end product properties being prepared are stable, long shelf-life；It is high in the wetability of crop surface after sprinkling, the drug effect lasting period
It is long；It directly acts on the fat-body of insect larvae and midgut epithelial cellses core, and replicate causes larva to catch an illness death rapidly, while can
To trigger epidemic disease in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively control pest population and its
Harm.Different from the insecticide of microbial source, killing speed of the present invention to insect is rapid, and half is only needed since the dead worm that sprays to
It is to the time.
Neem seed obtains pre-preg：Commercially available neem seed is taken, goes the removal of impurity, is crushed, crosses 20 mesh sieves, adds 15 parts of steamings
Distilled water, stir 30min, sealing and standing 8h.
The addition of petroleum ether is 1.2 times of the distilled water volume added during explosion.
Embodiment 2：
Insecticide prepared by microbial fermentation, composition and its parts by weight are 8 parts of bacillus thruingensis powder, 12 parts of neem seed, synergist 2
Part, 2 parts of the tuber of stemona, 3 parts of fragrant eupatorium herb, 6 parts of Eupatorium adenophorum.Synergist is 50% hemp oil and 50% organic silicon surfactant.
Hemp oil extracting method is：By the fresh ramie plant shearing of collection, crush, cross 50 mesh sieves, take ramie plant
Powder, the acetone of 5 times of volumes is added, stirring and leaching 7h, is centrifuged, be concentrated under reduced pressure to obtain hemp oil.
The method of insecticide prepared by microbial fermentation, comprises the following steps：
1）Pretreatment：Will neem seed carry out pre-preg after carry out steam blasting, Stress control in 3.5MPa, hold time for
50s, obtain neem seed explosion thing；Petroleum ether extraction is added, layering takes phase, obtains neem seed extract；
2）Fermentation：0.58g/L peptones 1.2mg/ is added in formula ratio Stemona Root, fragrant eupatorium herb powder and Eupatorium adenophorum powder
L Beta-alanines, cellulase producing strain is inoculated with, in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, high speed
Centrifuging and taking zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits cellulase-producing fungi bacterial strains
Separation screening and optimization culture conditions [J] Gansu agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2- in 8-15.
3；
3）It is prepared by finished product：Neem seed extract, formula ratio bacillus thruingensis powder and synergist are added in zymotic fluid, after stirring
The 1/6 of original volume is concentrated into, obtains the insecticide of microbial fermentation preparation.In the present invention there is obvious mutually association in each composition
Tune acts on, and the end product properties being prepared are stable, long shelf-life；It is high in the wetability of crop surface after sprinkling, the drug effect lasting period
It is long；It directly acts on the fat-body of insect larvae and midgut epithelial cellses core, and replicate causes larva to catch an illness death rapidly, while can
To trigger epidemic disease in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively control pest population and its
Harm.Different from the insecticide of microbial source, killing speed of the present invention to insect is rapid, and half is only needed since the dead worm that sprays to
It is to the time.
Neem seed obtains pre-preg：Commercially available neem seed is taken, goes the removal of impurity, is crushed, crosses 20 mesh sieves, adds 20 parts of steamings
Distilled water, stir 30min, sealing and standing 8h.
The addition of petroleum ether is 1.2 times of the distilled water volume added during explosion.
Embodiment 3：
Insecticide prepared by microbial fermentation, composition and its parts by weight are 15 parts of bacillus thruingensis powder, 10 parts of neem seed, synergist 2
Part, 3 parts of the tuber of stemona, 3 parts of fragrant eupatorium herb, 6 parts of Eupatorium adenophorum.Synergist is 40% hemp oil and 60% organic silicon surfactant.
Hemp oil extracting method is：By the fresh ramie plant shearing of collection, crush, cross 50 mesh sieves, take ramie plant
Powder, the acetone of 5 times of volumes is added, stirring and leaching 7h, is centrifuged, be concentrated under reduced pressure to obtain hemp oil.
The method of insecticide prepared by microbial fermentation, Optimization Steps：
1）Pretreatment：Will neem seed carry out pre-preg after carry out steam blasting, Stress control in 3.5MPa, hold time for
50s, obtain neem seed explosion thing；Petroleum ether solution extraction is added, layering takes phase, obtains neem seed extract.Wherein
Petroleum ether solution is the 4-phenylbutyrate dissolved with 0.1% in petroleum ether, compared to pure petroleum ether, petroleum ether solution pair of the invention
The extraction quantity of nimbin is more；
2）Fermentation：0.6g/L peptones 1.2mg/L is added in formula ratio Stemona Root, fragrant eupatorium herb powder and Eupatorium adenophorum powder
Beta-alanine, be inoculated with cellulase producing strain, in 25 DEG C, initial pH6.0,130r/min shake flask fermentation culture 72h, at a high speed from
The heart takes zymotic fluid.Above-mentioned cellulase producing strain is Wei Jiao, Wan Xuerui, Wu Run, waits point of cellulase-producing fungi bacterial strains
From screening and optimization culture conditions [J] Gansu agricultural university journal, 2016,51 (2):Aspergillus fumigatus bacterial strain B-2-3 in 8-15.；
3）It is prepared by finished product：Neem seed extract, formula ratio bacillus thruingensis powder and synergist are added in zymotic fluid, after stirring
The 1/6 of original volume is concentrated into, obtains the insecticide of microbial fermentation preparation.In the present invention there is obvious mutually association in each composition
Tune acts on, and the end product properties being prepared are stable, long shelf-life；It is high in the wetability of crop surface after sprinkling, the drug effect lasting period
It is long；It directly acts on the fat-body of insect larvae and midgut epithelial cellses core, and replicate causes larva to catch an illness death rapidly, while can
To trigger epidemic disease in population by laterally infection, infected by longitudinal direction and kill pupa and ovum, effectively control pest population and its
Harm.Different from the insecticide of microbial source, killing speed of the present invention to insect is rapid, and half is only needed since the dead worm that sprays to
It is to the time.
Neem seed obtains pre-preg：Commercially available neem seed is taken, goes the removal of impurity, is crushed, crosses 20 mesh sieves, adds 18 parts of steamings
Distilled water, stir 30min, sealing and standing 8h.
The addition of petroleum ether is 1.3 times of the distilled water volume added during explosion.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
Claims (7)
1. insecticide prepared by microbial fermentation, it is characterised in that composition and its parts by weight are 8 ~ 15 parts of bacillus thruingensis powder, print chinaberry kind
8 ~ 15 parts of son, 1 ~ 3 part of synergist, 1 ~ 3 part of the tuber of stemona, 1 ~ 3 part of fragrant eupatorium herb, 3 ~ 6 parts of Eupatorium adenophorum.
2. insecticide prepared by microbial fermentation according to claim 1, it is characterised in that：Described synergist be 40 ~
60% hemp oil and 40 ~ 60% organic silicon surfactants.
3. insecticide prepared by microbial fermentation according to claim 2, it is characterised in that：Described ramie oil extract side
Method is：By the fresh ramie plant shearing of collection, crush, cross 20-80 mesh sieves, take ramie plant powder, add acetone, stirring
5-9h is extracted, is centrifuged, be concentrated under reduced pressure to obtain hemp oil.
4. insecticide prepared by microbial fermentation according to claim 1, it is characterised in that：Described insecticide preparation side
Method comprises the following steps：
1）Pretreatment：Steam blasting is carried out after neem seed is carried out into pre-preg, Stress control is in 3.0 ~ 4.0MPa, maintenance
Between be 40 ~ 70s, obtain neem seed explosion thing；Petroleum ether extraction is added, layering takes phase, obtains neem seed extract；
2）Fermentation：In formula ratio Stemona Root, fragrant eupatorium herb powder and Eupatorium adenophorum powder add 0.5 ~ 0.6g/L peptones and
0.7 ~ 1.6mg/L Beta-alanines, are inoculated with cellulase producing strain, shake flask fermentation 60 ~ 84h of culture, and high speed centrifugation takes zymotic fluid；
3）It is prepared by finished product：Neem seed extract, formula ratio bacillus thruingensis powder and synergist are added in zymotic fluid, after stirring
The 1/6 ~ 1/4 of original volume is concentrated into, obtains the insecticide of microbial fermentation preparation.
5. insecticide prepared by microbial fermentation according to claim 4, it is characterised in that：Described neem seed obtains pre-
Leaching is handled：Commercially available neem seed is taken, goes the removal of impurity, is crushed, crosses 15-20 mesh sieves, adds 15-20 part distilled water, stirs 30-
35min, sealing and standing 6-10h.
6. insecticide prepared by microbial fermentation according to claim 4, it is characterised in that：The addition of described petroleum ether
0.7 ~ 1.5 times of the distilled water volume for measuring to add during explosion.
7. insecticide prepared by microbial fermentation according to claim 4, it is characterised in that：Described shake flask fermentation culture
Condition is：22 ~ 28 DEG C of fermentation temperature, initial pH5.0 ~ 7.0,100 ~ 200r/min of shaking flask rotary speed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711018451.8A CN107810994A (en) | 2017-10-27 | 2017-10-27 | Insecticide prepared by microbial fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711018451.8A CN107810994A (en) | 2017-10-27 | 2017-10-27 | Insecticide prepared by microbial fermentation |
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| Publication Number | Publication Date |
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| CN107810994A true CN107810994A (en) | 2018-03-20 |
Family
ID=61604382
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| CN201711018451.8A Withdrawn CN107810994A (en) | 2017-10-27 | 2017-10-27 | Insecticide prepared by microbial fermentation |
Country Status (1)
| Country | Link |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011061749A8 (en) * | 2009-11-19 | 2012-05-31 | Laila Nutraceuticals | Agents derived from holoptelea integrifolia and their compositions for the control of metabolic syndrome and associated diseases |
| CN103931666A (en) * | 2014-04-03 | 2014-07-23 | 福建省农业科学院植物保护研究所 | Insecticide synergist |
| CN107279196A (en) * | 2017-06-27 | 2017-10-24 | 浦江县元寿农业科技有限公司 | Plant source environment-friendly pesticide |
-
2017
- 2017-10-27 CN CN201711018451.8A patent/CN107810994A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011061749A8 (en) * | 2009-11-19 | 2012-05-31 | Laila Nutraceuticals | Agents derived from holoptelea integrifolia and their compositions for the control of metabolic syndrome and associated diseases |
| CN103931666A (en) * | 2014-04-03 | 2014-07-23 | 福建省农业科学院植物保护研究所 | Insecticide synergist |
| CN107279196A (en) * | 2017-06-27 | 2017-10-24 | 浦江县元寿农业科技有限公司 | Plant source environment-friendly pesticide |
Non-Patent Citations (1)
| Title |
|---|
| 朴香兰: "《民族药物提取分离新技术》", 31 October 2011 * |
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