CN108037086A - A kind of method based on matrimony vine coat color value measure matrimony vine luteole acid dipalmitate content - Google Patents
A kind of method based on matrimony vine coat color value measure matrimony vine luteole acid dipalmitate content Download PDFInfo
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- CN108037086A CN108037086A CN201711227207.2A CN201711227207A CN108037086A CN 108037086 A CN108037086 A CN 108037086A CN 201711227207 A CN201711227207 A CN 201711227207A CN 108037086 A CN108037086 A CN 108037086A
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- matrimony vine
- acid dipalmitate
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- luteole
- color value
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Abstract
The present invention relates to a kind of method for measuring matrimony vine luteole acid dipalmitate content in matrimony vine, more particularly, to a kind of method based on matrimony vine coat color value measure matrimony vine luteole acid dipalmitate content, this method is measurement matrimony vine surface brightness L, redness a and yellowing b first, then matrimony vine epidermis saturation degree c values and colourity angle h are calculated, the content of luteole acid dipalmitate is obtained finally by SPSS linear fits；The method of the present invention simplicity, uniqueness, can quickly calculate matrimony vine luteole acid dipalmitate content.
Description
Technical field
The present invention relates to a kind of method for measuring matrimony vine luteole acid dipalmitate content in matrimony vine, more particularly, to one
Method of the kind based on matrimony vine coat color value measure matrimony vine luteole acid dipalmitate content.
Background technology
Matrimony vine is rare Chinese medicine and invigorant,《Sheng Nong's herbal classic》Record：" the hard muscles and bones of matrimony vine long term usage energy, resistance to cold and heat,
Make light of one's life by commiting suicide not old, be the top grade in Chinese medicine.”《Compendium of Materia Medica》" fruit of Chinese wolfberry is sweet flat and moistens, property nourishing, can kidney tonifying, moistening lung, life for record
Essence, QI invigorating, this is the medicine of flat benefit." medlar health preserving historical origin for a long time, nowadays more paid much attention to be subject to experts and scholars.Matrimony vine
Carotenoid is one of active ingredient important in matrimony vine, and there is prevention age-related macular venereal disease to become, is anti-oxidant, antitumor, improve
Eyesight etc. acts on, and is also added as pigment and antioxidant in food.Carotenoid content in matrimony vine is dry fruit
0.03-0.5%, in addition to a small amount of luteole, beta carotene, beta-cryptoxanthin, more than 97% carotenoid is all to be esterified
Form exists, and what wherein content was most is luteole acid dipalmitate, accounts for more than the 75% of total carotinoid content, is Chinese holly
Feature carotenoid in Qi, the important indicator that can be judged as matrimony vine quality breeding and quality.
Luteole acid dipalmitate is the carboxylate of luteole, luteole（Zeaxanthin, 3,3 '-dihydroxy-β is recklessly
Radish element）Also known as zeaxanthin, be carrotene containing oxygen derivative, be to be present in plant, the day in algae and photosynthetic bacteria body
Right fat-soluble pigment.Have in luteole molecule and hydroxyl is carried on 11 conjugated double bonds and end group, there is stronger antioxygen
Change activity, Scavenging ability, be the most effective free radical scavenger in addition to astaxanthin.Luteole cannot close in vivo
Into, it is necessary to from meal supplement, and specificity is present in macula retinae center, is the carotenoids that nature is widely present
Element, and lutein isomer each other, are one of major carotenoids in human blood and tissue, have vision protection
Effect.In addition, luteole also has pre- anti-cancer, the prevention effect such as cardiovascular and cerebrovascular disease and the relevant disease of glycolipid metabolism,
It is considered as the ideal medicament for preventing and treating disease caused by free radical.
The evaluation method of matrimony vine luteole acid dipalmitate content is there is no at present, mainly by using organic solvent such as oil
Content detection is carried out with high performance liquid chromatography after the extraction such as ether, acetone, tetrahydrofuran, ether, n-hexane, not only extraction step is numerous
It is trivial, and injury of the organic solvent to human body is larger in operating process.There is presently no the method for overcoming this defect.
The content of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of easy method, uniqueness, can quickly calculate matrimony vine
One kind of luteole acid dipalmitate content is based on matrimony vine coat color value measure matrimony vine luteole acid dipalmitate content
Method.
The present invention realizes in the following way：
A kind of method based on matrimony vine coat color value measure matrimony vine luteole acid dipalmitate content, it is characterised in that：Should
Then method calculates matrimony vine epidermis saturation degree c values and colourity angle to measure matrimony vine surface brightness L, redness a and yellowing b first
H, the content of luteole acid dipalmitate is obtained finally by SPSS linear fits；
The step of measurement matrimony vine surface brightness L, redness a and yellowing b, is as follows：
1）Choose 9 common matrimony vine kinds, its ripe Chinese wolfberry fruit of random acquisition；
2）Matrimony vine coat color value is measured with spectrophotometric color measurement instrument, is rectified an instrument before measure with white correction ceramic tile, when measure is each
Matrimony vine sample randomly selects 5 fruits, measures 2 points at the axis of fruit surface of color value, obtains L, a, b value；
The matrimony vine epidermis saturation degree c values and colourity angle h values calculate according to the following formula (1) and (2),
(1)；
(2) ；
The method of the SPSS linear fits is as follows：
1）Above-mentioned 5 g of Chinese wolfberry fruit or so is weighed, each sample surveys three groups, adds liquid nitrogen grinding after adding three BHT, with 10 mL tetra-
10 min of hydrogen furans ultrasonic extraction, filtering and collecting filter liquid, filter residue is extracted to colourless with identical extraction process, after merging filtrate,
Add 20 g anhydrous sodium sulfate dewatering and filterings, be evaporated in 35 DEG C of concentrations, then dissolved with dichloromethane and be settled to 25 mL, -80
DEG C save backup；
2）With the content of high performance liquid chromatography detection luteole acid dipalmitate, wherein chromatographic condition used：Mobile phase A phase：
Methanol：Acetonitrile：Water：Triethylamine=81：14：5：0.08（v/v）, B phases：Methyl tertiary butyl ether(MTBE)：Dichloromethane=1：1（v/v）；Gradient
Elution, elution program：0 min, 84% A, 16% B；22 min, 83% A, 17% B；40 min, 45% A, 55% B； 55
Min, 25% A, 75% B；60 min, 84% A, 16% B；70 min, 84% A, 16% B；1 mL/min of flow velocity；Detection wavelength
450 nm；25 DEG C of column temperature, 20 μ L of sample size；
3）If the estimation equation Y of luteole acid dipalmitate content（mg/100g）=β 0+ β 1L+ β 2a+β 3b+ β 4c+ β
5h, wherein β 0, β 1 ..., β 5 are regression coefficients, and with L, a, b, c, h are independent variable, and the content Y of luteole acid dipalmitate is
Dependent variable, it is respectively -582.221,0.739 to carry out linear regression analysis to estimate β 0, β 1 ..., β 5 using SPSS,
8.028, -2.868,1.380,6.944, then the content estimation equation of luteole acid dipalmitate is Y（mg/100g）=-
582.221+0.739*L+8.028*a -2.868*b+1.380*c+6.944*h；
The spectrophotometric color measurement instrument is Konica Minolta CM-5.
The present invention has the effect that：
1）Method is easy, unique：Method provided by the invention is measures matrimony vine surface brightness L, redness a and yellowing b first, then
Matrimony vine epidermis saturation degree c values and colourity angle h are calculated, luteole acid dipalmitate is obtained finally by SPSS linear fits
Content.The present invention provides a kind of Modulus Model that its luteole acid dipalmitate content is estimated using matrimony vine color feature value.
First, matrimony vine color characteristic analysis is carried out using Konica Minolta CM-5 spectrophotometric color measurement instruments, measure is preceding to correct ceramic tile with white
Rectify an instrument, each matrimony vine sample randomly selects 5 fruits during measure, 2 points of color value, measurement at the axis of measurement fruit surface
It is averaged after obtaining matrimony vine surface color value L, a and b, then calculates c values and h values according to formula (1), (2), while with HPLC methods
The content of luteole acid dipalmitate in matrimony vine is measured, luteole acid dipalmitate is then obtained by SPSS linear fits
Five color values of content and matrimony vine epidermis regression equation it is as follows：The content Y of luteole acid dipalmitate（mg/100g）
=-582.221+ 0.739*L+8.028*a -2.868*b+1.380*c+6.944*h（P=0.006, fitting
Value R2=0.993）.This regression equation can be used for calculating matrimony vine luteole acid dipalmitate after the color value measured is brought into
Content.
2）The beneficial effects of the invention are as follows using regression equation, the double palm fibres of luteole in matrimony vine are estimated with the color value of matrimony vine epidermis
The content of glycerin monostearate.Method utilizes formula to measure matrimony vine epidermis colour L, a, b value with spectrophotometric color measurement instrument first（1）With（2）Meter
C, h value are calculated, then L, a, b, c, the above-mentioned regression equation of h values substitution can be calculated into containing for matrimony vine luteole acid dipalmitate
Amount.
3）The present invention establishes a kind of quick judgment method of matrimony vine luteole acid dipalmitate content, and it is organic to avoid tradition
Organic solvent pollution caused by solvent extraction, detection, the system of luteole acid dipalmitate reference substance when having saved each experiment
It is standby, testing cost is reduced, saves the time, improves detection efficiency.Suitable in the high-quality selection and breeding of matrimony vine and quality evaluation
The quick judgement of luteole acid dipalmitate content in journey.Embodiment
Embodiment one：A kind of method based on matrimony vine coat color value measure matrimony vine luteole acid dipalmitate content, including
Following steps：
Step 1, collection matrimony vine coat color data：
1）Choose 9 common matrimony vine kinds, its ripe Chinese wolfberry fruit of random acquisition；
2）Matrimony vine coat color value is measured with spectrophotometric color measurement instrument, is rectified an instrument before measure with white correction ceramic tile, when measure is each
Matrimony vine sample randomly selects 5 fruits, measures 2 points at the axis of fruit surface of color value, obtains L, a, b value；
3）According to formula（1）With（2）Matrimony vine epidermis saturation degree c values and colourity angle h values are calculated,
(1)；
(2) 。
The content of luteole acid dipalmitate in step 2, the above-mentioned Chinese wolfberry fruit of measure：
1）Above-mentioned 5 g of Chinese wolfberry fruit or so is weighed, each sample surveys three groups, adds liquid nitrogen grinding after adding three BHT, with 10 mL tetra-
10 min of hydrogen furans ultrasonic extraction, filtering and collecting filter liquid, filter residue is extracted to colourless with identical extraction process, after merging filtrate,
Add 20 g anhydrous sodium sulfate dewatering and filterings, be evaporated in 35 DEG C of concentrations, then dissolved with dichloromethane and be settled to 25 mL, -80
DEG C save backup.
2）With the content of high performance liquid chromatography detection luteole acid dipalmitate, wherein chromatographic condition used：Mobile phase A phase：
Methanol：Acetonitrile：Water：Triethylamine=81：14：5：0.08（v/v）, B phases：Methyl tertiary butyl ether(MTBE)：Dichloromethane=1：1（v/v）；Gradient
Elution, elution program：0 min, 84% A, 16% B；22 min, 83% A, 17% B；40 min, 45% A, 55% B； 55
Min, 25% A, 75% B；60 min, 84% A, 16% B；70 min, 84% A, 16% B；1 mL/min of flow velocity；Detection wavelength
450 nm；25 DEG C of column temperature, 20 μ L of sample size.
3）The double palmitic acids of luteole are calculated according to the peak area of the luteole acid dipalmitate of above-mentioned acquisition and standard curve
The content Y of ester（mg/100g）
The different matrimony vine coat color values of table 1 and luteole acid dipalmitate content
Step 3, linear regression analysis draw estimation equation.
1）If the estimation equation of luteole acid dipalmitate content is Y（mg/100g）=β 0+ β 1L+ β 2a+β 3b+ β 4c
+ β 5h, wherein β 0, β 1 ..., β 5 are regression coefficients.With L, a, b, c, h are independent variable, the content of luteole acid dipalmitate
Y is dependent variable, and it is respectively -582.221,0.739 to carry out linear regression analysis to estimate β 0, β 1 ..., β 5 using SPSS,
8.028, -2.868,1.380,6.944, then the content estimation equation of luteole acid dipalmitate is Y（mg/100g）=-
582.221+0.739*L+8.028*a -2.868*b+1.380*c+6.944*h.
2）After SPSS linear regression analyses, by observing the coefficient of determination 0.960 after adjusting, the goodness of fit is higher, dependent variable base
Originally can be explained by independent variable.It is 0.006 by the probability of regression equation significance test, less than significance 0.05, then recognizes
Linear relationship for dependent variable and independent variable entirety is significant, therefore the linear equation established can use.
Claims (5)
- A kind of 1. method based on matrimony vine coat color value measure matrimony vine luteole acid dipalmitate content, it is characterised in that： Then this method calculates matrimony vine epidermis saturation degree c values and colourity to measure matrimony vine surface brightness L, redness a and yellowing b first Angle h, the content of luteole acid dipalmitate is obtained finally by SPSS linear fits.
- 2. a kind of as claimed in claim 1 measure matrimony vine luteole acid dipalmitate content based on matrimony vine coat color value Method, it is characterised in that：The step of measurement matrimony vine surface brightness L, redness a and yellowing b, is as follows：1）Choose 9 common matrimony vine kinds, its ripe Chinese wolfberry fruit of random acquisition；2）Matrimony vine coat color value is measured with spectrophotometric color measurement instrument, is rectified an instrument before measure with white correction ceramic tile, when measure is each Matrimony vine sample randomly selects 5 fruits, measures 2 points at the axis of fruit surface of color value, obtains L, a, b value.
- 3. a kind of as claimed in claim 1 measure matrimony vine luteole acid dipalmitate content based on matrimony vine coat color value Method, it is characterised in that：The matrimony vine epidermis saturation degree c values and colourity angle h values calculate according to the following formula (1) and (2),(1)；(2) 。
- 4. a kind of as claimed in claim 1 measure matrimony vine luteole acid dipalmitate content based on matrimony vine coat color value Method, it is characterised in that：The method of the SPSS linear fits is as follows：1）Above-mentioned 5 g of Chinese wolfberry fruit or so is weighed, each sample surveys three groups, adds liquid nitrogen grinding after adding three BHT, with 10 mL tetra- 10 min of hydrogen furans ultrasonic extraction, filtering and collecting filter liquid, filter residue is extracted to colourless with identical extraction process, after merging filtrate, Add 20 g anhydrous sodium sulfate dewatering and filterings, be evaporated in 35 DEG C of concentrations, then dissolved with dichloromethane and be settled to 25 mL, -80 DEG C save backup；2）With the content of high performance liquid chromatography detection luteole acid dipalmitate, wherein chromatographic condition used：Mobile phase A phase： Methanol：Acetonitrile：Water：Triethylamine=81：14：5：0.08（v/v）, B phases：Methyl tertiary butyl ether(MTBE)：Dichloromethane=1：1（v/v）；Gradient Elution, elution program：0 min, 84% A, 16% B；22 min, 83% A, 17% B；40 min, 45% A, 55% B； 55 Min, 25% A, 75% B；60 min, 84% A, 16% B；70 min, 84% A, 16% B；1 mL/min of flow velocity；Detection wavelength 450 nm；25 DEG C of column temperature, 20 μ L of sample size；3）If the estimation equation Y of luteole acid dipalmitate content（mg/100g）=β 0+ β 1L+ β 2a+β 3b+ β 4c+ β 5h, wherein β 0, β 1 ..., β 5 are regression coefficients, and with L, a, b, c, h are independent variable, and the content Y of luteole acid dipalmitate is Dependent variable, it is respectively -582.221,0.739 to carry out linear regression analysis to estimate β 0, β 1 ..., β 5 using SPSS, 8.028, -2.868,1.380,6.944, then the content estimation equation of luteole acid dipalmitate is Y（mg/100g）=- 582.221+0.739*L+8.028*a -2.868*b+1.380*c+6.944*h.
- 5. a kind of as claimed in claim 1 measure matrimony vine luteole acid dipalmitate content based on matrimony vine coat color value Method, it is characterised in that：The spectrophotometric color measurement instrument is Konica Minolta CM-5.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110715900A (en) * | 2018-07-11 | 2020-01-21 | 四川省中医药科学院 | Method for detecting content of main chemical components of turmeric according to color of turmeric powder |
| CN111307976A (en) * | 2020-03-12 | 2020-06-19 | 宁波大学 | High-flux detection method for carotenoid in aquatic product |
| CN111426635A (en) * | 2020-04-07 | 2020-07-17 | 陈海锋 | Method for detecting conversion rate of magnesium oxide to magnesium hydroxide |
| CN111426634A (en) * | 2019-11-21 | 2020-07-17 | 四川省中医药科学院 | Method for detecting content of main chemical components of salvia miltiorrhiza medicinal material powder according to color of salvia miltiorrhiza medicinal material powder |
| CN112485358A (en) * | 2020-12-14 | 2021-03-12 | 宁夏医科大学 | Method for simultaneously and rapidly qualitatively and quantitatively detecting multiple active ingredients in wolfberry medicinal material |
| CN112858533A (en) * | 2021-03-23 | 2021-05-28 | 贵州中烟工业有限责任公司 | Method for measuring plastid pigment content in tobacco shreds |
-
2017
- 2017-11-29 CN CN201711227207.2A patent/CN108037086A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| A. ZULUETA ET.AL: "Carotenoids and Color of Fruit Juice and Milk Beverage Mixtures", 《FOOD CHEMISTRY AND TOXICOLOGY》 * |
| CONCEPCIO Ä N SA Ä NCHEZ-MORENO ET.AL: "Vitamin C, Provitamin A Carotenoids, and Other Carotenoids in High-Pressurized Orange Juice during Refrigerated Storage", 《JOURNAL OF AGRICULTURE AND FOOD CHEMISTRY》 * |
| 傅利平等著: "《公共管理研究方法》", 31 July 2015 * |
| 米 佳等: "枸杞色泽与其类胡萝卜素含量和组成的相关性", 《食品科学》 * |
| 贺帆等: "基于色度学的密集烘烤过程中烟叶主要化学成分变化模型研究", 《西北农林科技大学学报（自然科学版）》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110715900A (en) * | 2018-07-11 | 2020-01-21 | 四川省中医药科学院 | Method for detecting content of main chemical components of turmeric according to color of turmeric powder |
| CN111426634A (en) * | 2019-11-21 | 2020-07-17 | 四川省中医药科学院 | Method for detecting content of main chemical components of salvia miltiorrhiza medicinal material powder according to color of salvia miltiorrhiza medicinal material powder |
| CN111307976A (en) * | 2020-03-12 | 2020-06-19 | 宁波大学 | High-flux detection method for carotenoid in aquatic product |
| CN111307976B (en) * | 2020-03-12 | 2022-06-28 | 宁波大学 | High-throughput detection method for carotenoid in aquatic product |
| CN111426635A (en) * | 2020-04-07 | 2020-07-17 | 陈海锋 | Method for detecting conversion rate of magnesium oxide to magnesium hydroxide |
| CN112485358A (en) * | 2020-12-14 | 2021-03-12 | 宁夏医科大学 | Method for simultaneously and rapidly qualitatively and quantitatively detecting multiple active ingredients in wolfberry medicinal material |
| CN112485358B (en) * | 2020-12-14 | 2022-05-20 | 宁夏医科大学 | Method for simultaneously and rapidly qualitatively and quantitatively detecting multiple active ingredients in wolfberry medicinal material |
| CN112858533A (en) * | 2021-03-23 | 2021-05-28 | 贵州中烟工业有限责任公司 | Method for measuring plastid pigment content in tobacco shreds |
| CN112858533B (en) * | 2021-03-23 | 2022-09-30 | 贵州中烟工业有限责任公司 | Method for measuring plastid pigment content in tobacco shreds |
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