CN107996390A - A kind of method of big fragrance of a flower Chinese rose hybridization cultivation - Google Patents
A kind of method of big fragrance of a flower Chinese rose hybridization cultivation Download PDFInfo
- Publication number
- CN107996390A CN107996390A CN201711290590.6A CN201711290590A CN107996390A CN 107996390 A CN107996390 A CN 107996390A CN 201711290590 A CN201711290590 A CN 201711290590A CN 107996390 A CN107996390 A CN 107996390A
- Authority
- CN
- China
- Prior art keywords
- chinese rose
- flower
- pollen
- pollination
- hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of method of big fragrance of a flower Chinese rose hybridization cultivation, it is characterised in that including following aspect：（1）Pollen preserves, and selects in shape flower Chinese rose that pattern is bright-coloured, the large flower of build shape, collects shape flower Chinese rose pollen using sterile conical flask, is preserved after baking is handled；（2）Activation, preserves under 18 20 DEG C of environment, and uses activating solution atomization process；（3）Pollination is handled, and sterilization sprinkling is carried out to big fragrance of a flower Chinese rose with 75% ethanol, is wrapped up pollination flower using transparent plastic bag after pollination, then spray activating solution bagging culture；（4）Hybridization, spends Chinese rose pollen and big fragrance of a flower Chinese rose pollen to carry out pollination hybridization with hybridizing big fragrance of a flower Chinese rose using shape respectively；（5）4 generation seeds are placed in progress constant temperature plantation in greenhouse, Post flowering carries out pollination in kind by breeding in kind.
Description
Technical field
The invention belongs to big fragrance of a flower Chinese rose planting technology field, and in particular to a kind of side of big fragrance of a flower Chinese rose hybridization cultivation
Method.
Background technology
Big fragrance of a flower Chinese rose, also known as huge colored rose, bloom kind, its pattern is varied, robust plant, flower pattern for a season property
Graceful grace, pattern are numerous bright-coloured lucid and lively, have stronger aromatic odor and ornamental value, and its flower can be additionally used in perfume and carry
Raw material is taken, there is higher economic value.But the Hybrid Tea florescence only respectively opens once in annual spring and summer, relative to other moons
Kind each season in season blooms, its number of blooming is few, flower low output, and big fragrance of a flower Chinese rose plantation economic benefit is limited to；Greatly
Fragrance of a flower Chinese rose is mainly grown in height above sea level 1400-1700m high altitude environments, is not suitable for plains region plantation, its cultivated areas is subject to
Limitation, it is impossible to which large-scale promotion is planted.And shape flower Chinese rose forms for tea perfume Chinese rose and poly- flower Chinese rose hybridization, its growth ability is strong,
Robust plant, flower amount is big, the frequency of blooming is high, and has stronger disease and insect resistance and winter resistance, can change as excellent hybridization
Non-defective unit kind.And conventionally employed pollination hybridizing method, loss of activity causes pollination success rate low during its pollen preserves, and in pollination
Influenced by conditions such as temperature, humidity, rainwater and illumination, pollination success rate can be adversely affected；And by once hybridizing
The inhereditary feature that method is obtained, its stability is poor, it is few to obtain inhereditary feature, it is impossible to effectively plays to colored improving effect.
The content of the invention
The present invention is directed to the problem of existing：In terms of yield, the Hybrid Tea florescence only respectively opens once in annual spring and summer, it is bloomed
Number is few, flower low output, and big fragrance of a flower Chinese rose plantation economic benefit is limited to.Regional condition, big fragrance of a flower Chinese rose mainly grow
In height above sea level 1400-1700m high altitude environments, it is not suitable for plains region plantation, its cultivated areas is restricted, it is impossible to is pushed away on a large scale
Extensive farming is planted.Hybridizing method, and conventionally employed pollination hybridizing method, loss of activity causes pollination success rate low during its pollen preserves,
And influenced by conditions such as temperature, humidity, rainwater and illumination in pollinating, pollination success rate can be adversely affected；And lead to
The inhereditary feature that a hybridizing method is obtained is crossed, its stability is poor, it is few to obtain inhereditary feature, it is impossible to effectively plays and colored is changed
Good action.To solve the problems, such as that above-mentioned is that the present invention provides a kind of method of big fragrance of a flower Chinese rose hybridization cultivation.
The present invention is achieved by the following technical solutions：
A kind of method of big fragrance of a flower Chinese rose hybridization cultivation, comprises the following steps：
（1）Pollen preserves：Select in shape flower Chinese rose that pattern is bright-coloured, the large flower of build shape, its pollen amount and activity are high, use nothing
Bacterium conical flask collects shape flower Chinese rose pollen, is preserved after baking is handled, is made and preserves pollen；Stage cooling can avoid temperature
Difference is excessive to be caused to damage to pollen activity, and low temperature method can effectively extend pollen holding time and activity, to time of pollination and
Condition has more choices, improves pollination success rate；
（2）Activation：It will preserve at a temperature of pollen is placed in 8-12 DEG C and place 1-2h, 2-3h is then preserved under 18-20 DEG C of environment, it is extensive
The activity of pollen is preserved again, and uses activating solution atomization process 20-25min, can improve moisture in pollen, contained amino
The components such as acid, vitamin, folic acid, can improve pollen activity and the different ability of resistance, and activation pollen is made；
（3）Pollination is handled：Sterilization sprinkling is carried out to big fragrance of a flower Chinese rose with the ethanol that volumetric concentration is 75%, reduces pathogenic bacteria to awarding
The influence of powder, reuses small brushes and dips pollen and pollinate, and then stands 3- using the transparent plastic bag flower parcel that will pollinate
4h, influence of the factors such as rainwater, wind to pollination is avoided while sufficient sunlight is kept, and improves pollination success rate, then spray
Polybag is removed after spilling the culture of activating solution bagging 3-4 days, 1 generation seed is obtained after ripe；
（4）Hybridization：Shape flower Chinese rose pollen pollination hybridization will be reused after 1 generation cultivating seeds, obtains 2 generation seeds, then by 2 generation seed institutes
Cultivate flower to pollinate with big fragrance of a flower Chinese rose pollen, 3 generation seeds are obtained after cultivation, reuse shape and spend Chinese rose pollen to 3 generation seed institutes
Flower pollination hybridization is cultivated, 4 generation seeds are obtained after cultivation；Repeatedly spend Chinese rose pollen progress miscellaneous with shape by that must hybridize for 1 generation after pollination
Hand over, big fragrance of a flower Chinese rose after hybridization can be made to obtain more excellent inhereditary features, and keep the stability of inhereditary feature, and by with
Big fragrance of a flower Chinese rose pollination improves itself excellent inhereditary feature in Hybrid；
（5）Breeding in kind：4 generation seeds are placed in progress constant temperature plantation in greenhouse, Post flowering carries out kind of an interior pollination, and gained seed can
Large-scale planting is carried out, after pollination culture in kind, its inhereditary feature has higher stability, can carry out large-scale promotion kind
Plant.
Step（1）The baking, its temperature are 37-42 DEG C, time 34-38min.
Step（1）The preservation, its method are：- 5--3 DEG C of holding 2-3h are first cooled the temperature to, then are down to -15--12
DEG C preserved for a long time.
Step（2）The activating solution, wherein being respectively configured to sub-prime gauge part and being：
72-76 parts of water, 14-18 parts of corn juice, 6-9 parts of yeast extract, 3-5 parts of vitamin C, 2-5 parts of folic acid, sodium selenate
0.2-0.4 parts, 0.1-0.2 parts of gibberellin.
Step（3）The transparent plastic bag, it has two air holes in bottom, its diameter is 7-10mm.
Step（5）The constant temperature plantation, its temperature is 15-20 DEG C, humidity 62%-65%, and carbon dioxide volumetric concentration is
0.04%-0.06%, ozone volumetric concentration are 0.01%-0.02%.
The present invention has the following advantages compared with prior art：Pollen preserve, select the large flower of build shape, its pollen amount and
It is active high, pollen pollination success rate can be improved, moisture in pollen is reduced after low temperature is baked, avoids pollen in Cord blood
Freeze, pollen activity is damaged；And pollen is preserved by two stage coolings, it can avoid reducing excessive temperature differentials pair
Damage, low temperature method can effectively extend pollen holding time and activity caused by pollen activity, have to the time and condition of pollination
There are more choices, improve pollination success rate.Activation method, pollen is stood at a temperature of 18-20 DEG C can recover to preserve
Pollen activity, and atomization process is carried out using activating solution, moisture in pollen on the one hand can be improved, improves its activity, separately
Containing components such as amino acid, plant hormone, vitamins in one side activating solution, pollen activity, the resistance opposite sex can be improved.At pollination
Reason method, is sterilized before pollinating using 75% ethanol, and reducing pathogenic bacteria influences pollination, and transparent plastic bag is used after pollination
Bagging processing, you can make pollination spend to obtain the sunlight of abundance, and influence of the factors such as rainwater, wind to pollination can be avoided, and spray
Activating solution provides nutrition supply and improves pollen activity, so as to improve pollination success rate.Hybridizing method, by that must hybridize after pollination
In 1 generation, was repeatedly hybridized with shape flower Chinese rose pollen, and big fragrance of a flower Chinese rose obtains more excellent inhereditary features after can making hybridization, and protects
The stability of inhereditary feature is held, the Optimality of big fragrance of a flower Chinese rose and shape flower Chinese rose is merged；But after repeatedly hybridization, greatly
Serious loss occurs in possessed excellent inhereditary feature to fragrance of a flower Chinese rose in itself, by 2 the seed flower moon in generation big fragrance of a flower Chinese rose
Pollination is cultivated, and can keep itself excellent flower properties.4 generation seeds are carried out greenhouse cultivation, through in kind by mating system in kind
Pollination is cultivated, and can be obtained with the seed for stablizing inhereditary feature, be carried out large-scale promotion plantation.
Embodiment
Embodiment 1：
A kind of method of big fragrance of a flower Chinese rose hybridization cultivation, comprises the following steps：
（1）Pollen preserves：Select in shape flower Chinese rose that pattern is bright-coloured, the large flower of build shape, its pollen amount and activity are high, use nothing
Bacterium conical flask collects shape flower Chinese rose pollen, is preserved after baking is handled, is made and preserves pollen；Stage cooling can avoid temperature
Difference is excessive to be caused to damage to pollen activity, and low temperature method can effectively extend pollen holding time and activity, to time of pollination and
Condition has more choices, improves pollination success rate；
（2）Activation：Preservation pollen is placed at a temperature of 9 DEG C and places 1.5h, 2.5h is then preserved under 19 DEG C of environment, recovers to preserve
The activity of pollen, and activating solution atomization process 22min is used, moisture in pollen, contained amino acid, vitamin can be improved
Deng component, pollen activity and the different ability of resistance can be improved, activation pollen is made；
（3）Pollination is handled：Sterilization sprinkling is carried out to big fragrance of a flower Chinese rose with the ethanol that volumetric concentration is 75%, reduces pathogenic bacteria to awarding
The influence of powder, reuses small brushes and dips pollen and pollinate, and is then stood using the transparent plastic bag flower parcel that will pollinate
3.5h, influence of the factors such as rainwater, wind to pollination is avoided while sufficient sunlight is kept, and improves pollination success rate, then
The culture of sprinkling activating solution bagging removes polybag after 3 days, 1 generation seed is obtained after ripe；
（4）Hybridization：Shape flower Chinese rose pollen pollination hybridization will be reused after 1 generation cultivating seeds, obtains 2 generation seeds, then by 2 generation seed institutes
Cultivate flower to pollinate with big fragrance of a flower Chinese rose pollen, 3 generation seeds are obtained after cultivation, reuse shape and spend Chinese rose pollen to 3 generation seed institutes
Flower pollination hybridization is cultivated, 4 generation seeds are obtained after cultivation；Repeatedly spend Chinese rose pollen progress miscellaneous with shape by that must hybridize for 1 generation after pollination
Hand over, big fragrance of a flower Chinese rose after hybridization can be made to obtain more excellent inhereditary features, and keep the stability of inhereditary feature, and by with
Big fragrance of a flower Chinese rose pollination improves itself excellent inhereditary feature in Hybrid；
（5）Breeding in kind：4 generation seeds are placed in progress constant temperature plantation in greenhouse, Post flowering carries out kind of an interior pollination, and gained seed can
Large-scale planting is carried out, after pollination culture in kind, its inhereditary feature has higher stability, can carry out large-scale promotion kind
Plant.
Step（1）The baking, its temperature are 38 DEG C, time 35min.
Step（1）The preservation, its method are：- 3.5 DEG C of holding 2.5h are first cooled the temperature to, then are down to -13 DEG C of progress
It is long-term to preserve.
Step（2）The activating solution, wherein being respectively configured to sub-prime gauge part and being：
73 parts of water, 15 parts of corn juice, 7 parts of yeast extract, 3.6 parts of vitamin C, 3 parts of folic acid, 0.26 part of sodium selenate, gibberellin
0.13 part.
Step（3）The transparent plastic bag, it has two air holes in bottom, its diameter is 7-10mm.
Step（5）The constant temperature plantation, its temperature are 16 DEG C, and humidity 63%, carbon dioxide volumetric concentration is 0.042%,
Ozone volumetric concentration is 0.014%.
Embodiment 2：
For the present embodiment 2 compared with embodiment 1, step change is main in the following areas：
Step（1）The baking, its temperature are 41 DEG C, time 37min.
Step（1）The preservation, its method are：- 4.5 DEG C of holding 3h are first cooled the temperature to, then is down to -14 DEG C and is grown
Phase preserves.
Step（2）The activating solution, wherein being respectively configured to sub-prime gauge part and being：
75 parts of water, 17 parts of corn juice, 8 parts of yeast extract, 4.7 parts of vitamin C, 4 parts of folic acid, 0.38 part of sodium selenate, gibberellin
0.19 part.
Step（2）Activation, its method are：
Preserve at a temperature of pollen is placed in 11 DEG C and place 2h, 3h is then preserved under 20 DEG C of environment, and use activating solution atomization process
24min。
Step（3）The transparent plastic bag, it has two air holes in bottom, its diameter is 7-10mm.
Step（5）The constant temperature plantation, its temperature are 19 DEG C, and humidity 64%, carbon dioxide volumetric concentration is 0.054%,
Ozone volumetric concentration is 0.018%.
Contrast 1：
This contrast 1 does not carry out step compared with embodiment 1（1）Middle store method, other steps are same as Example 1.
Contrast 2：
This contrast 2 does not carry out step compared with embodiment 1（2）Middle activating solution uses, other steps are same as Example 1.
Contrast 3：
This contrast 3 does not carry out step compared with embodiment 2（3）Pollination is handled, other steps are same as Example 2.
Contrast 4：
This contrast 4 does not carry out step compared with embodiment 2（4）Middle hybridizing method, other steps are same as Example 2.
Control group：
Shape flower Chinese rose pollen after collection is directly carried out pollination hybridization by control group to big fragrance of a flower Chinese rose, and store method, work is not used
Change liquid, pollination processing and hybridizing method.
To embodiment 1, embodiment 2,1, contrast 2, contrast 3, contrast 4 and control group experimental program are contrasted, after statistics hybridizes
Bloom number, Plain plantation survival rate, pollination success rate, time at florescence of big fragrance of a flower Chinese rose is compared.
Experimental data：
| Project | Bloom time for several times | Plain plantation survival rate % | Success rate of pollinating % | Time at florescence d |
| Embodiment 1 | 4 | 91.56 | 87.69 | 15 |
| Embodiment 2 | 4 | 90.87 | 87.34 | 14 |
| Contrast 1 | 2 | 89.45 | 83.44 | 15 |
| Contrast 2 | 2 | 88.39 | 84.18 | 14 |
| Contrast 3 | 3 | 85.41 | 81.62 | 11 |
| Contrast 4 | 3 | 86.29 | 87.25 | 12 |
| Control group | 2 | 76.25 | 74.21 | 9 |
Synthesis result：What the present invention was cultivated cries big fragrance of a flower Chinese rose again, and compared with the control group, it blooms number increase twice, puts down
Original area's plantation survival rate improves 15.31%, and pollination success rate improves 13.48%, florescence time lengthening 6d.Use preservation and activation
Liquid method, its Plain plantation survival rate improve 2.11%, 3.17%, and pollination success rate improves 4.25%, 3.51%, florescence time lengthening
0d、1d；And using pollination processing and hybridizing method, number of blooming improves 1 time, 1 time, Plain plantation survival rate raising 5.46%,
4.58%, pollination success rate improves 5.72%, 0%, florescence time lengthening 3d, 2d.
Claims (6)
- A kind of 1. method of big fragrance of a flower Chinese rose hybridization cultivation, it is characterised in that comprise the following steps：（1）Pollen preserves：Select in shape flower Chinese rose that pattern is bright-coloured, the large flower of build shape, collect shape using sterile conical flask and spend the moon Season pollen, preserved after baking is handled, be made preserve pollen；（2）Activation：It will preserve at a temperature of pollen is placed in 8-12 DEG C and place 1-2h, 2-3h is then preserved under 18-20 DEG C of environment, and Using activating solution atomization process 20-25min, activation pollen is made；（3）Pollination is handled：Sterilization sprinkling is carried out to big fragrance of a flower Chinese rose with the ethanol that volumetric concentration is 75%, small brushes is reused and dips in Take pollen to pollinate, pollination flower parcel is then stood into 3-4h using transparent plastic bag, then spray activating solution bagging culture Polybag is removed after 3-4 days, 1 generation seed is obtained after ripe；（4）Hybridization：Shape flower Chinese rose pollen pollination hybridization will be reused after 1 generation cultivating seeds, obtains 2 generation seeds, then by 2 generation seed institutes Cultivate flower to pollinate with big fragrance of a flower Chinese rose pollen, 3 generation seeds are obtained after cultivation, reuse shape and spend Chinese rose pollen to 3 generation seed institutes Flower pollination hybridization is cultivated, 4 generation seeds are obtained after cultivation；（5）Breeding in kind：4 generation seeds are placed in progress constant temperature plantation in greenhouse, Post flowering carries out kind of an interior pollination, and gained seed can Carry out large-scale planting.
- 2. the method for big fragrance of a flower Chinese rose hybridization cultivation as claimed in claim 1, it is characterised in that step（1）The baking, Its temperature is 37-42 DEG C, time 34-38min.
- 3. the method for big fragrance of a flower Chinese rose hybridization cultivation as claimed in claim 1, it is characterised in that step（1）The preservation, Its method is：- 5--3 DEG C of holding 2-3h are first cooled the temperature to, then is down to -15--12 DEG C and is preserved for a long time.
- 4. the method for big fragrance of a flower Chinese rose hybridization cultivation as claimed in claim 1, it is characterised in that step（2）The activation Liquid, wherein being respectively configured to sub-prime gauge part and being：72-76 parts of water, 14-18 parts of corn juice, 6-9 parts of yeast extract, 3-5 parts of vitamin C, 2-5 parts of folic acid, sodium selenate 0.2-0.4 parts, 0.1-0.2 parts of gibberellin.
- 5. the method for big fragrance of a flower Chinese rose hybridization cultivation as claimed in claim 1, it is characterised in that step（3）Described is transparent Polybag, it has two air holes in bottom, its diameter is 7-10mm.
- 6. the method for big fragrance of a flower Chinese rose hybridization cultivation as claimed in claim 1, it is characterised in that step（5）The constant temperature Plantation, its temperature are 15-20 DEG C, humidity 62%-65%, and carbon dioxide volumetric concentration is 0.04%-0.06%, and ozone volumetric concentration is 0.01%-0.02%。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711290590.6A CN107996390A (en) | 2017-12-08 | 2017-12-08 | A kind of method of big fragrance of a flower Chinese rose hybridization cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711290590.6A CN107996390A (en) | 2017-12-08 | 2017-12-08 | A kind of method of big fragrance of a flower Chinese rose hybridization cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107996390A true CN107996390A (en) | 2018-05-08 |
Family
ID=62057165
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711290590.6A Pending CN107996390A (en) | 2017-12-08 | 2017-12-08 | A kind of method of big fragrance of a flower Chinese rose hybridization cultivation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107996390A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258456A (en) * | 2018-11-06 | 2019-01-25 | 兰州市农业科技研究推广中心 | A kind of method of China's bitter water rose artificial pollination |
| CN109832187A (en) * | 2019-04-11 | 2019-06-04 | 贵州师范大学 | A kind of method of HERBA DENDROBII artificial hybridization |
| CN116267592A (en) * | 2023-05-17 | 2023-06-23 | 东北林业大学 | Cold-resistant China rose and hybridization cultivation method thereof |
-
2017
- 2017-12-08 CN CN201711290590.6A patent/CN107996390A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258456A (en) * | 2018-11-06 | 2019-01-25 | 兰州市农业科技研究推广中心 | A kind of method of China's bitter water rose artificial pollination |
| CN109832187A (en) * | 2019-04-11 | 2019-06-04 | 贵州师范大学 | A kind of method of HERBA DENDROBII artificial hybridization |
| CN116267592A (en) * | 2023-05-17 | 2023-06-23 | 东北林业大学 | Cold-resistant China rose and hybridization cultivation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101690461B (en) | Method for preparing triploid plants | |
| CN104885773B (en) | A kind of method of quickly breeding blue berry early stage sizing tissue culture commercial seedling | |
| CN103081807B (en) | Method for regenerating plant by use of callus of camellia japonica | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN102835252B (en) | Culture method of clitocybe maxima | |
| CN101218896A (en) | Method for producing momordica grosvenori group seedling with single culture medium | |
| CN107996390A (en) | A kind of method of big fragrance of a flower Chinese rose hybridization cultivation | |
| CN106577284B (en) | A kind of method of yellow lady's-slipper tissue-culturing rapid propagation and florescence control | |
| CN105087400B (en) | A kind of artificial culture method of chinquapin advantage fungal component root fungus | |
| CN109122303A (en) | A method of improving capsicum hybrid seeding efficiency and yield | |
| CN106332716A (en) | Camphor tree cuttage method | |
| CN102210259A (en) | Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization | |
| CN101263783B (en) | Method for using herbicide barban to fast breed aloe all-male plant strain | |
| CN107079817A (en) | Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method | |
| CN107311719A (en) | A kind of greenhouse gardening method of hickory chick | |
| CN105210824A (en) | The equipment of the sub-seedling of mist training strawberry stolon and mist culture method thereof | |
| CN105230483A (en) | Method for establishing in-vitro regeneration system of Osmunda vachellii | |
| CN108684511A (en) | The cultural method of greenhouse organic matrix type soilless culture thick-skinned melon | |
| CN104351000A (en) | Culture method of pomelo hybrid | |
| CN103461099A (en) | Method for creating novel white flesh loquat strain by taking red flesh loquat variety as material | |
| CN101341854B (en) | Cultivation method for vegetative organ in vitro cluster bud of adult tree of mandarin orange | |
| CN104876736B (en) | A kind of bottle plants tulip nutrient solution | |
| CN107810819A (en) | A kind of breeding method of Chinese herbaceous peony new varieties | |
| CN107094615A (en) | A kind of hybridizing method for overcoming lily interspecific hybridization obstacle | |
| CN104221629A (en) | Conical redpepper fruit vegetative propagation seedling method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180508 |
|
| WD01 | Invention patent application deemed withdrawn after publication |