CN109258456A - A kind of method of China's bitter water rose artificial pollination - Google Patents
A kind of method of China's bitter water rose artificial pollination Download PDFInfo
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- CN109258456A CN109258456A CN201811314527.6A CN201811314527A CN109258456A CN 109258456 A CN109258456 A CN 109258456A CN 201811314527 A CN201811314527 A CN 201811314527A CN 109258456 A CN109258456 A CN 109258456A
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- pollination
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- bitter water
- water rose
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The present invention provides a kind of methods of Chinese bitter water rose artificial pollination, mainly include the following steps: that (1) bud acquires: selecting the plant of the rose variety of merit as male parent, select the plant of bitter water rose as female parent, win whole male parent bud；(2) anther kept dry: the male parent bud won is opened, fresh anther is removed；(3) pollen collecting: after the pollen on anther is scattered naturally, pollen is collected；(4) it pollinates: when maternal bud will burst forth, dipping a small amount of paternal pollen and be applied on maternal bud column cap；(5) bagging: pollination bag is inserted on the maternal flower that pollination is completed and is sealed；(6) it removes bag: after waiting one period, removing pollination bag.The present invention is self-incompatible for bitter water rose the study found that proposing a kind of bitter water rose as maternal removes male artificial hybridization pollinating method from, and this method is easy to operate, time saving and energy saving, success rate of breeding height.
Description
Technical field
The invention belongs to plant hybridization breeding technical fields, are related to a kind of method of Chinese bitter water rose artificial pollination.
Background technique
Rose is the machaka of rosaceae (Rosa rugosa) Rosa (Rosaceae), misery, mountain in China Gansu
There is a cultivation of larger area on ground such as yin, Liaoning, Yunnan and Xinjiang of conquering east, the byproducts such as rose and its essential oil it is ornamental, medicinal,
Edible, daily use chemicals etc. have valuable value.The main breed of the current rose in China is " polyphyll rose " and " bitter water rose ".
" bitter water rose " (R.sertata X R.rugosa Yu. Et.Ku.) be rose Yu cognate rose Natural hybrid, initially
It breeds and promotes in Gansu Province Yongdeng County misery town, and titled with place name.Existing more than 200 years cultivation histories at present, in local cultivation
Training area has reached 180,000 mu, and bitter water rose industry becomes local specialty industries.
For a long time, the breeding of bitter water rose is based on vegetative propagation technique, including plant division and mound layering, and in recent years
Come the Technique on Cuttage Propagation technically haveing breakthrough.But long-term vegetative propagation reduces bitter water rose resistance year by year, influences
The further promotion of its yield and quality, and due to single variety, constrain the development of local rose industry.
Make full use of existing Rosa Rugosa Germplasm Resource, training (choosing) bring out the florescence is long, yield is high, oil yield is high, disease resistance is strong,
Rose variety best in quality has particularly important meaning for the sound development of rose industry.Currently, rose commonly educates
Kind method has: (1) introduction and acclimatization；(2) selection and use, including bud mutation selection and seed selection of growing directly from seeds；(3) crossbreeding；(4) other are educated
Kind method (including selfing breeding, radioactive breeding etc.).The Chinese rose belonged to rose is as the first big ornamental plant in the world, mostly
Number kinds are obtained by hybridizing to cultivate for a long time, and therefore, crossbreeding is a kind of important channel and the method for rose breeding,
And during crossbreeding, pollination link is most important.Currently, there has been no bitter water rose artificial hybridization breeding pollination techniques
Report.
Summary of the invention
It is an object of the invention in view of the problems of the existing technology, provide a kind of Chinese bitter water rose artificial pollination
Method, to realize the crossbreeding and breed improvement of bitter water rose.
For this purpose, the present invention adopts the following technical scheme:
A kind of method of China's bitter water rose artificial pollination, includes the following steps:
(1) bud acquires: selecting the plant of the rose variety of merit as male parent according to breeding objective, selects bitter water rose
Plant as maternal, whole flower flower bud is won when male parent bud sufficiently expands；
(2) anther kept dry: the male parent bud won is opened, fresh anther is removed and places 12-24h at room temperature；
(3) pollen collecting: after the pollen on anther is scattered naturally, pollen is collected；
(4) it pollinates: when maternal bud will burst forth, gently petal being opened, a small amount of paternal pollen is dipped and is applied to female parent
On bud column cap；
(5) bagging: pollination bag is inserted on the maternal flower that pollination is completed and is sealed；
(6) it removes bag: after waiting one period, removing pollination bag, count percentage of fertile fruit and setting percentage.
Further, the deadline of step (4) selects in morning 9:00~11:00.
Further, in step (5), pollination time and male parent information are indicated on pollination bag after the completion of bagging, if whole strain
When bitter water rose is pollinated with same male parent, it is listed in plant bottom and indicates relevant information.
Further, in step (5), the pollination bag selects sulfuric acid paper bag.
Further, in step (6), after maternal plant fruit setting and after fruit maturation, then pollination bag is removed.Selection
Pollination bag is removed after fruit maturation, is because rose carries out nutrient growth after July, branches and leaves are dense, if without pollination bag
It is easy to be blocked by dense branches and leaves after fruit maturation, pollination bag and rose branches and leaves color difference are more obvious, therefore retain to fruit
After maturation, convenient for identifying and picking.
Through applicants have found that, the male of bitter water rose be it is sterile, can pollination when exempt emasculation the step of, directly
It is pollinated with the paternal pollen of selection, this is that previous research institute is unwitnessed.Therefore, step (4) of the present invention is awarded
The operation of emasculation is omitted when powder, directly pollinates.The following are the male sterile verifying (parents of bitter water rose that applicant is done
With property measurement) test:
1. experimental design
Compatibility measurement sets 5 processing, and A: bagging (spontaneous pollination) is not pollinated in not emasculation；B: not emasculation self-pollination bagging (from
Flower pollination)；C: bagging (single-female generation) after emasculation；D: different strain pollinates bagging (cross-pollination)；E: (bitter water rose is for xenogenesis pollination
Male parent, purple branch rose are female parent)；Compare CK: (bitter water rose is female parent to xenogenesis pollination bagging, and purple branch rose is father after emasculation
This).30 single flowers of each processing count ovary increasing rate after one month.
Ovary increasing rate (%)=ovary increasing number/total pollination flower number × 100%
As a result, it has been found that when Parent is bitter water rose, whether emasculation has no son in artificial pollination and spontaneous pollination
Room expands, and ovary increasing rate is 0；When bitter water rose is male parent, and purple branch rose is maternal, ovary increasing rate is 0；Control is hardship
Water rose is female parent, and when purple branch rose is male parent, ovary increasing rate is 1) 86.67%(is shown in Table.Test result shows: bitter water rose
Male sterility, and cannot row single-female generation, belong to the not affine plant of complete selfing.
1 bitter water rose affinity determination of table
For the feasibility for further verifying the present invention program, for different male parent varieties, using method provided by the invention into
Row hybrid experiment, concrete outcome are as shown in table 2.
The percentage of fertile fruit and setting percentage statistics of the different rose cross combinations of table 2
In experiment, the rose variety as male parent has ' purple branch rose white ', ' Bulgarian red rose ', ' Shangshui rose ', ' polyphyll
Purple branch powder rose ', ' bur rose ', 7 kinds of ' polyphyll purple branch red rose ' and ' Liaoning four seasons rose ' etc., the results show that " Shangshui rose
It is rare " percentage of fertile fruit highest as male parent, the average percentage of fertile fruit combined up to 93.33%, 7 kind is up to 79.11%.Contained by each fruit
From the point of view of number seeds, the higher combination of setting percentage has ' purple branch rose white ', ' Bulgarian red rose ', ' Shangshui rose ' and ' weight
Valve purple branch powder rose ', for seed contained by every piece of fruit more than 10, the average setting percentages of 7 kinds of combinations are 9.4.Illustrate to utilize
The method of the present invention obtains preferable percentage of fertile fruit and setting percentage.
Further to verify the influence that pollination time hybridizes bitter water rose, the present invention has also carried out different pollination times pair
The influence of bitter water rose setting percentage is tested, and concrete outcome is shown in Table 3.
Influence of the different pollination times of table 3 to bitter water rose setting percentage
Seen from table 3, it pollinates respectively in morning time section and, pollinated compared with afternoon in the morning 9:00~11:00 the period in the afternoon
14:00~16:00, which pollinates, can significantly improve the number of results and setting percentage of bitter water rose, it is therefore preferable that in morning 9:00~11:
00 pollinates.
The beneficial effects of the present invention are: it is self-incompatible for bitter water rose the study found that providing a kind of misery
Rose removes male artificial hybridization pollinating method from as maternal, and this method is easy to operate, time saving and energy saving, and success rate of breeding is high.
Specific embodiment
The method of the present invention is described further below by specific embodiment.
A kind of method of China's bitter water rose artificial pollination, includes the following steps:
(1) bud acquires: according to breeding objective, selecting the plant of the rose variety of merit as male parent, selects misery rose
It is rare to be used as female parent, whole flower flower bud is won when male parent bud sufficiently expands；
(2) anther kept dry: male parent bud is opened, and is removed fresh anther and is contained on smooth and clean template, in
12-24h is placed at room temperature；
(3) it pollen collecting: after the pollen on template is scattered naturally, is packed into centrifuge tube and seals up for safekeeping；
(4) it pollinates: when maternal bud will burst forth, gently petal being opened, a small amount of paternal pollen is dipped with cotton swab and is applied to
On the column cap of maternal bud, and select to complete to pollinate in morning 9:00~11:00；
(5) bagging: with sulfuric acid paper bag (5cm*5cm) be inserted in pollination complete flower on, sealed with stapler, at the same with remember
Number pen is in the bright pollination time of sulfuric acid paper bag subscript and male parent information, if whole strain bitter water rose is pollinated with same male parent,
It is listed in plant bottom and indicates relevant information；
(6) it removes bag: after maternal plant fruit setting and to fruit maturation, winning sulfuric acid paper bag.
Claims (5)
1. a kind of method of China's bitter water rose artificial pollination, which comprises the steps of:
(1) bud acquires: selecting the plant of the rose variety of merit as male parent according to breeding objective, selects bitter water rose
Plant as maternal, whole flower flower bud is won when male parent bud sufficiently expands；
(2) anther kept dry: the male parent bud won is opened, fresh anther is removed and places 12-24h at room temperature；
(3) pollen collecting: after the pollen on anther is scattered naturally, pollen is collected；
(4) it pollinates: when maternal bud will burst forth, gently petal being opened, a small amount of paternal pollen is dipped and is applied to female parent
On bud column cap；
(5) bagging: pollination bag is inserted on the maternal flower that pollination is completed and is sealed；
(6) it removes bag: after waiting one period, removing pollination bag.
2. a kind of method of Chinese bitter water rose artificial pollination according to claim 1, which is characterized in that step (4)
Deadline selects in morning 9:00~11:00.
3. a kind of method of Chinese bitter water rose artificial pollination according to claim 1, which is characterized in that in step (5),
Pollination time and male parent information are indicated on pollination bag after the completion of bagging, if whole strain bitter water rose is pollinated with same male parent
When, it is listed in plant bottom and indicates relevant information.
4. a kind of method of Chinese bitter water rose artificial pollination according to claim 1, which is characterized in that in step (5),
The pollination bag selects sulfuric acid paper bag.
5. a kind of method of Chinese bitter water rose artificial pollination according to claim 1, which is characterized in that in step (6),
After maternal plant fruit setting and after fruit maturation, then remove pollination bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811314527.6A CN109258456B (en) | 2018-11-06 | 2018-11-06 | Artificial pollination method for Chinese rosa laevigata |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811314527.6A CN109258456B (en) | 2018-11-06 | 2018-11-06 | Artificial pollination method for Chinese rosa laevigata |
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| CN109258456A true CN109258456A (en) | 2019-01-25 |
| CN109258456B CN109258456B (en) | 2022-04-08 |
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| CN201811314527.6A Active CN109258456B (en) | 2018-11-06 | 2018-11-06 | Artificial pollination method for Chinese rosa laevigata |
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Citations (5)
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|---|---|---|---|---|
| EP2128274A4 (en) * | 2007-03-23 | 2010-07-07 | Int Flower Dev Pty Ltd | Method for determination of presence of crossing with garden-species rose plant in wild-species rose plant |
| CN102550396A (en) * | 2012-02-22 | 2012-07-11 | 云南省农业科学院花卉研究所 | Method for quickly identifying cross-compatibility of rose hybrida |
| CN103891509A (en) * | 2014-03-28 | 2014-07-02 | 兰州市农业科技研究推广中心 | Cuttage of Chinese Kushui rose |
| CN105409760A (en) * | 2015-12-04 | 2016-03-23 | 贵州省植物园 | Method for improving cross pollination success rate of Chinese rose |
| CN107996390A (en) * | 2017-12-08 | 2018-05-08 | 凤阳县中益绿洲花木种植专业合作社 | A kind of method of big fragrance of a flower Chinese rose hybridization cultivation |
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2018
- 2018-11-06 CN CN201811314527.6A patent/CN109258456B/en active Active
Patent Citations (5)
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|---|---|---|---|---|
| EP2128274A4 (en) * | 2007-03-23 | 2010-07-07 | Int Flower Dev Pty Ltd | Method for determination of presence of crossing with garden-species rose plant in wild-species rose plant |
| CN102550396A (en) * | 2012-02-22 | 2012-07-11 | 云南省农业科学院花卉研究所 | Method for quickly identifying cross-compatibility of rose hybrida |
| CN103891509A (en) * | 2014-03-28 | 2014-07-02 | 兰州市农业科技研究推广中心 | Cuttage of Chinese Kushui rose |
| CN105409760A (en) * | 2015-12-04 | 2016-03-23 | 贵州省植物园 | Method for improving cross pollination success rate of Chinese rose |
| CN107996390A (en) * | 2017-12-08 | 2018-05-08 | 凤阳县中益绿洲花木种植专业合作社 | A kind of method of big fragrance of a flower Chinese rose hybridization cultivation |
Non-Patent Citations (6)
| Title |
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| T. DEBENER等: "Use of diploid self incompatible rose genotypes as a tool for gene flow analyses", 《PLANT BREEDING》 * |
| 张明菊: "《园林植物遗传育种》", 31 July 2004, 中国农业出版社 * |
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