CN107988407B - 两种树形梨不同发育时期叶组织的荧光定量内参基因及其引物和应用 - Google Patents
两种树形梨不同发育时期叶组织的荧光定量内参基因及其引物和应用 Download PDFInfo
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- CN107988407B CN107988407B CN201711227896.7A CN201711227896A CN107988407B CN 107988407 B CN107988407 B CN 107988407B CN 201711227896 A CN201711227896 A CN 201711227896A CN 107988407 B CN107988407 B CN 107988407B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
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Abstract
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CN201711227896.7A CN107988407B (zh) | 2017-11-29 | 2017-11-29 | 两种树形梨不同发育时期叶组织的荧光定量内参基因及其引物和应用 |
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CN201711227896.7A CN107988407B (zh) | 2017-11-29 | 2017-11-29 | 两种树形梨不同发育时期叶组织的荧光定量内参基因及其引物和应用 |
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CN107988407A CN107988407A (zh) | 2018-05-04 |
CN107988407B true CN107988407B (zh) | 2018-11-06 |
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CN201711227896.7A Active CN107988407B (zh) | 2017-11-29 | 2017-11-29 | 两种树形梨不同发育时期叶组织的荧光定量内参基因及其引物和应用 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108070673B (zh) * | 2018-01-17 | 2021-05-18 | 湖北省农业科学院果树茶叶研究所 | 两种树形梨不同树体部位叶组织的荧光定量内参基因及其引物和应用 |
CN111118198B (zh) * | 2020-01-20 | 2022-07-15 | 福建农林大学 | 一组梨果皮qRT-PCR内参基因及其引物和应用 |
CN111118197B (zh) * | 2020-01-20 | 2022-07-15 | 福建农林大学 | 一组梨果肉qRT-PCR内参基因及其引物和应用 |
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2017
- 2017-11-29 CN CN201711227896.7A patent/CN107988407B/zh active Active
Non-Patent Citations (4)
Title |
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MG029160.1;Liu等;《NCBI,GenBank》;20171025;全文以及TITLE部分 * |
Systematic selection and validation of appropriate reference genes for gene expression studies by quantitative real-time PCR in pear;Yuanyuan Xu等;《Acta Physiol Plant》;20150130;第37卷(第40期);摘要部分 * |
XM_009353469.1;无;《NCBI,GenBank》;20161012;全文 * |
梨茎尖中PpKO 基因表达量的实时荧光定量PCR 分析;田义轲等;《华北农学报》;20121231;第27卷(第3期);全文 * |
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CN107988407A (zh) | 2018-05-04 |
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Application publication date: 20180504 Assignee: Hubei Yifanberry Ecological Agriculture Technology Co.,Ltd. Assignor: INSTITUTE OF FRUIT AND TEA, HUBEI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2022420000082 Denomination of invention: Fluorescence quantification of internal reference genes in leaf tissues of two tree-shaped pears at different developmental stages and their primers and applications Granted publication date: 20181106 License type: Common License Record date: 20220907 |
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Application publication date: 20180504 Assignee: Jingshan Nianhe Ecological Agriculture Co.,Ltd. Assignor: INSTITUTE OF FRUIT AND TEA, HUBEI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2022420000088 Denomination of invention: Fluorescence quantification of internal reference genes in leaf tissues of two tree-shaped pears at different developmental stages and their primers and applications Granted publication date: 20181106 License type: Common License Record date: 20220913 |