CN107974394A - A kind of instrument for extracting nucleic acid - Google Patents

A kind of instrument for extracting nucleic acid Download PDF

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Publication number
CN107974394A
CN107974394A CN201810058189.8A CN201810058189A CN107974394A CN 107974394 A CN107974394 A CN 107974394A CN 201810058189 A CN201810058189 A CN 201810058189A CN 107974394 A CN107974394 A CN 107974394A
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China
Prior art keywords
bar magnet
guide rail
fuselage
nucleic acid
instrument
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CN201810058189.8A
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Chinese (zh)
Inventor
周志图
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Wuxi Hundred Biotechnology Co Ltd
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Wuxi Hundred Biotechnology Co Ltd
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Priority to CN201810058189.8A priority Critical patent/CN107974394A/en
Publication of CN107974394A publication Critical patent/CN107974394A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides a kind of instrument for extracting nucleic acid, including fuselage, magnetic frame, bar magnet, bar magnet stock, bar magnet set, drip deflector, deep-well plates, positioning seat, turntable, control panel.Wherein, guide rail is provided with the vertical direction of the fuselage, the guide rail includes the first guide rail and the second guide rail, and first guide rail and second guide rail parallel are arranged on the fuselage.By the control panel can electrodeless adjustment vibrate, mixing and magnetic speed, the magnetic frame and the bar magnet stock are double mode, and the deep-well plates are common deep-well plates.By the present invention with solve the problems, such as instrument for extracting nucleic acid bar magnet position offset existing in the prior art, calibration frequently, operating mode is single, deep-well plates waste, can not accurately control vibration, mixing, magnetic speed.

Description

A kind of instrument for extracting nucleic acid
Technical field
The present invention relates to a kind of nucleic acid extraction field, and in particular to a kind of instrument for extracting nucleic acid.
Background technology
Nucleic acids research is the important topic in modern biology, medical research.Carrier of the nucleic acid as hereditary information, is The material base of gene expression, except normally grow, develop and breed in organism etc. in vital movement have it is particularly significant Effect outside, its abnormal conditions with life, such as tumour generation, radiation insult, genetic disease also have substantial connection.
Instrument for extracting nucleic acid, is the instrument that the extraction work of sample nucleic acid is automatically performed using supporting nucleic acid extracting reagent Device, instrument for extracting nucleic acid are divided into two classes:One kind is large-scale automation, commonly referred to as automated fluid work station;Another kind of is small The automatic instrument for extracting nucleic acid of type, extraction purification process is automatically performed using packaged matched reagent.Large-scale automated fluid work station Because equipment cost is high, operating cost is high, is adapted to once extract thousands of a one species samples, so be really applied It is fewer.And the instrument of small automatic, it is easy to operate to be widely used because instrument and equipment and operating cost are low.
At present, there are following defect and deficiency for the common instrument for extracting nucleic acid of in the market:(1) track of magnetic frame is single track Road, it is fixed unstable, it be easy to cause magnetic bar position and deviation occurs, need to often calibrates;(2) vibration, mixing and magnetic speed can not Adjust, or can only adjust fast, slow, be unfavorable for optimizing for different samples;(3) supporting deep-well plates can not flexible modulation Flux, when the sample size of processing is less, can cause the waste of 96 hole depth orifice plates, add cost;(4) model of magnetic bar It can not change, pattern switching can not be carried out, when sample pipetting volume volume to be treated is excessive, experiment can not be flexibly met will Ask.
The content of the invention
The present invention provides a kind of instrument for extracting nucleic acid, with solve instrument for extracting nucleic acid bar magnet position offset existing in the prior art, The problem of calibration is frequently, operating mode is single.
In order to solve the above technical problems, the present invention provides a kind of instrument for extracting nucleic acid, including fuselage, magnetic frame, bar magnet, bar magnet Stock, bar magnet set, drip deflector, deep-well plates, positioning seat, turntable, control panel.Wherein, set in the vertical direction of the fuselage Guide rail is equipped with, the magnetic frame and the bar magnet stock are arranged on the guide rail and the magnetic frame is located at the bar magnet stock Top, the drip deflector is arranged on the fuselage and positioned at the lower section of the bar magnet stock.The bar magnet is arranged on institute State on magnetic frame, the bar magnet is set on the bar magnet stock.The turntable is close in the fuselage and can freely turn It is dynamic, it is fixed with the turntable on the positioning seat and the positioning seat and is placed with the deep-well plates, the control panel is set Side in the fuselage relative to the magnetic frame.The guide rail includes the first guide rail and the second guide rail, first guide rail It is arranged in second guide rail parallel on the fuselage.Can electrodeless adjustment vibration, mixing and magnetic by the control panel Speed, the magnetic frame and the bar magnet stock are double mode, and the deep-well plates are common deep-well plates.
Preferably, the drip deflector is hinged on the fuselage and can move along the horizontal plane.
Preferably, the common deep-well plates include base, groove, cutting, and the base can be fitted on positioning seat, described The groove is provided with base and the cutting can be positioned in the groove.
Preferably, 6 positioning seats are provided with the turntable, and are set on wantonly one or two of adjacent positioning seat There is heating refrigeration module.
Preferably, the turntable center is provided with conducting ring, the heating refrigeration module by the conducting ring with it is described Control system connection on fuselage.
Preferably, the double mode is high throughput pattern and large capacity pattern.
Preferably, the high throughput pattern is that 96 bar magnets are provided with the magnetic frame and are arranged according to 12 × 8, 96 bar magnet sets are provided with the bar magnet stock and according to 12 × 8 arrangements, single hole volume is 2.2ml.
Preferably, the large capacity pattern is that 24 bar magnets are provided with the magnetic frame and are arranged according to 6 × 4, 24 bar magnet sets are provided with the bar magnet stock and according to 6 × 4 arrangements, single hole volume is 11ml.
The beneficial effect that the present invention is brought:A kind of instrument for extracting nucleic acid provided by the invention, by setting two-orbit to make magnetic force Frame and bar magnet stock are very steady when moving up and down, and are not in rock, and primary calibration can ensure long-time position Accuracy.Magnetic frame and bar magnet stock are double mode, can use different patterns according to the size of sample pipetting volume volume.Deep hole Plate is common deep-well plates, according to the quantity of sample, can flexibly select the quantity of cutting, is avoided using deep during high throughput pattern The waste of orifice plate, reduces experimental cost.
Brief description of the drawings
Fig. 1 is the overall structure diagram of instrument for extracting nucleic acid according to embodiments of the present invention.
Fig. 2 is the structure diagram of common deep-well plates according to embodiments of the present invention.
Wherein, 1- fuselages, 2- magnetic frames, 3- bar magnets, 4- bar magnet stocks, 5- bar magnet sets, 6- drip deflectors, 7- deep-well plates, 701- bases, 702- grooves, 703- cuttings, 8- positioning seats, 9- turntables, 10- heating refrigeration modules.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with drawings and the specific embodiments, to this Invention is described in further detail.
Fig. 1 is the overall structure diagram of instrument for extracting nucleic acid according to embodiments of the present invention.Fig. 2 is to implement according to the present invention The structure diagram of the common deep-well plates of example.
First embodiment:
A kind of instrument for extracting nucleic acid provided by the invention, including fuselage 1, magnetic frame 2, bar magnet 3, bar magnet stock 4, bar magnet set 5, Drip deflector 6, deep-well plates 7, positioning seat 8, turntable 9, control panel.Wherein, it is provided with and leads in the vertical direction of the fuselage 1 Rail, the magnetic frame 2 and the bar magnet stock 4 are arranged on the guide rail and the magnetic frame 2 is located at the bar magnet stock 4 Top, the drip deflector 6 are arranged on the fuselage 1 and positioned at the lower section of the bar magnet stock 4.The bar magnet 3 is arranged on On the magnetic frame 2, the bar magnet set 5 is arranged on the bar magnet stock 4.The turntable 9 is close in the fuselage 1 and can be with It is freely rotated, is fixed with the turntable 9 on the positioning seat 8 and the positioning seat 8 and is placed with the deep-well plates 7, the control Panel processed is arranged on side of the fuselage 1 relative to the magnetic frame 2.The guide rail includes the first guide rail and the second guide rail, First guide rail and second guide rail parallel are arranged on the fuselage 1.The magnetic frame 2 and 4 energy of bar magnet stock Enough it is securely fixed on first guide rail and second guide rail, the magnetic frame 2 and the bar magnet stock 4 are moved down upper It is very steady when dynamic, be not in rock, primary calibration can ensure the accuracy of long-time position.Pass through the chain of command Plate can electrodeless adjustment vibration, mixing and magnetic speed, using intelligent speed-regulating, make speed more accurate, be conducive to user according to Sample and magnetic bead situation optimize adjustment.The magnetic frame 2 and the bar magnet stock 4 are double mode, can be added according to sample The size of sample volume uses different patterns.The deep-well plates 7 are common deep-well plates, can be according to the quantity of sample, flexibly choosing The quantity of cutting is selected, the waste of the deep-well plates 7, reduces experimental cost when avoiding using high throughput pattern.
Furthermore, the drip deflector 6 is hinged on the fuselage 1 and can move along the horizontal plane.It is described common deep Orifice plate includes base 701, groove 702, cutting 703, and the base 701 can be fitted on positioning seat 8, be set on the base 701 It is equipped with the groove and the cutting 703 can be positioned in the groove 702.6 positioning seats are provided with the turntable 9 8, and heating refrigeration module 10 is provided with wantonly one or two of adjacent positioning seat 8.9 center of turntable is provided with conduction Ring, the heating refrigeration module 10 are connected by the conducting ring with the control system on the fuselage 1.
In the present embodiment, using high throughput pattern, the high throughput pattern is is provided with 96 institutes on the magnetic frame 2 State bar magnet 3 and arranged according to 12 × 8,96 bar magnets sets 5 are provided with the bar magnet stock 4 and according to 12 × 8 arrangements, it is single Pore volume is 2.2ml.
Second embodiment:
A kind of instrument for extracting nucleic acid provided by the invention, including fuselage 1, magnetic frame 2, bar magnet 3, bar magnet stock 4, bar magnet set 5, Drip deflector 6, deep-well plates 7, positioning seat 8, turntable 9, control panel.Wherein, it is provided with and leads in the vertical direction of the fuselage 1 Rail, the magnetic frame 2 and the bar magnet stock 4 are arranged on the guide rail and the magnetic frame 2 is located at the bar magnet stock 4 Top, the drip deflector 6 are arranged on the fuselage 1 and positioned at the lower section of the bar magnet stock 4.The bar magnet 3 is arranged on On the magnetic frame 2, the bar magnet set 5 is arranged on the bar magnet stock 4.The turntable 9 is close in the fuselage 1 and can be with It is freely rotated, is fixed with the turntable 9 on the positioning seat 8 and the positioning seat 8 and is placed with the deep-well plates 7, the control Panel processed is arranged on side of the fuselage 1 relative to the magnetic frame 2.The guide rail includes the first guide rail and the second guide rail, First guide rail and second guide rail parallel are arranged on the fuselage 1.The magnetic frame 2 and 4 energy of bar magnet stock Enough it is securely fixed on first guide rail and second guide rail, the magnetic frame 2 and the bar magnet stock 4 are moved down upper It is very steady when dynamic, be not in rock, primary calibration can ensure the accuracy of long-time position.Pass through the chain of command Plate can electrodeless adjustment vibration, mixing and magnetic speed, using intelligent speed-regulating, make speed more accurate, be conducive to user according to Sample and magnetic bead situation optimize adjustment.The magnetic frame 2 and the bar magnet stock 4 are double mode, can be added according to sample The size of sample volume uses different patterns.The deep-well plates 7 are common deep-well plates, can be according to the quantity of sample, flexibly choosing The quantity of cutting is selected, the waste of the deep-well plates 7, reduces experimental cost when avoiding using high throughput pattern.
Furthermore, the drip deflector 6 is hinged on the fuselage 1 and can move along the horizontal plane.It is described common deep Orifice plate includes base 701, groove 702, cutting 703, and the base 701 can be fitted on positioning seat 8, be set on the base 701 It is equipped with the groove and the cutting 703 can be positioned in the groove 702.6 positioning seats are provided with the turntable 9 8, and heating refrigeration module 10 is provided with wantonly one or two of adjacent positioning seat 8.9 center of turntable is provided with conduction Ring, the heating refrigeration module 10 are connected by the conducting ring with the control system on the fuselage 1.
In the present embodiment, using large capacity pattern, the large capacity pattern is is provided with 24 institutes on the magnetic frame 2 State bar magnet 3 and arranged according to 6 × 4,24 bar magnets sets 5 are provided with the bar magnet stock 4 and according to 6 × 4 arrangements, single hole Volume is 11ml.
In conclusion a kind of instrument for extracting nucleic acid provided by the invention, by setting two-orbit to make magnetic frame and bar magnet stock It is very steady when moving up and down, be not in rock, primary calibration can ensure the accuracy of long-time position.Magnetic frame It is double mode with bar magnet stock, different patterns can be used according to the size of sample pipetting volume volume.Deep-well plates are common deep hole Plate, according to the quantity of sample, can flexibly select the quantity of cutting, avoid the waste using deep-well plates during high throughput pattern, Reduce experimental cost.
The foregoing is merely the embodiment of the present invention, is not intended to limit the invention, for those skilled in the art For member, the invention may be variously modified and varied.Any modification within the spirit and principles of the invention, being made, Equivalent substitution, improvement etc., should be included within scope of the presently claimed invention.

Claims (8)

1. a kind of instrument for extracting nucleic acid, including fuselage (1), magnetic frame (2), bar magnet (3), bar magnet stock (4), bar magnet set (5), dropping liquid Baffle (6), deep-well plates (7), positioning seat (8), turntable (9), control panel, are provided with the vertical direction of the fuselage (1) and lead Rail, the magnetic frame (2) and the bar magnet stock (4) are arranged on the guide rail and the magnetic frame (2) is located at the bar magnet The top of stock (4), the drip deflector (6) are arranged on the fuselage (1) and are located at the lower section of the bar magnet stock (4), The bar magnet (3) is arranged on the magnetic frame (2), and the bar magnet set (5) is arranged on the bar magnet stock (4), described turn Disk (9) is close in the fuselage (1) and can be freely rotated, and the positioning seat (8) and described fixed is fixed with the turntable (9) The deep-well plates (7) are placed with position seat (8), the control panel is arranged on the fuselage (1) relative to the magnetic frame (2) Side, it is characterised in that the guide rail includes the first guide rail and the second guide rail, and first guide rail and second guide rail are put down Row be arranged on the fuselage (1), by the control panel can electrodeless adjustment vibrate, mixing and magnetic speed, the magnetic Power frame (2) and the bar magnet stock (4) are double mode, and the deep-well plates (7) are common deep-well plates.
2. instrument for extracting nucleic acid as claimed in claim 1, it is characterised in that the drip deflector (6) is hinged on the fuselage (1) It can move above and along the horizontal plane.
3. instrument for extracting nucleic acid as claimed in claim 1, it is characterised in that the common deep-well plates include base (701), groove (702), cutting (703), the base (701) can be fitted on positioning seat (8), be provided with the base (701) described recessed Groove and the cutting (703) can be positioned in the groove (702).
4. instrument for extracting nucleic acid as claimed in claim 1, it is characterised in that be provided with 6 positioning seats on the turntable (9) (8), and on wantonly one or two of adjacent positioning seat (8) it is provided with heating refrigeration module (10).
5. instrument for extracting nucleic acid as claimed in claim 1, it is characterised in that turntable (9) center is provided with conducting ring, described Heating refrigeration module (10) is connected by the conducting ring with the control system on the fuselage (1).
6. the instrument for extracting nucleic acid as described in Claims 1 to 5 is any, it is characterised in that the double mode for high throughput pattern and Large capacity pattern.
7. instrument for extracting nucleic acid as claimed in claim 6, it is characterised in that the high throughput pattern is on the magnetic frame (2) It is provided with 96 bar magnets (3) and is arranged according to 12 × 8,96 bar magnet sets (5) is provided with the bar magnet stock (4) And according to 12 × 8 arrangements, single hole volume is 2.2ml.
8. instrument for extracting nucleic acid as claimed in claim 6, it is characterised in that the large capacity pattern is on the magnetic frame (2) It is provided with 24 bar magnets (3) and is arranged according to 6 × 4,24 bar magnet sets (5) is provided with the bar magnet stock (4) And according to 6 × 4 arrangements, single hole volume is 11ml.
CN201810058189.8A 2018-01-22 2018-01-22 A kind of instrument for extracting nucleic acid Pending CN107974394A (en)

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Cited By (6)

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Publication number Priority date Publication date Assignee Title
CN108359581A (en) * 2018-05-02 2018-08-03 烟台德迈生物科技有限公司 A kind of device adsorbing mechanical shaking extraction biomolecule by magnetic bead
CN108424838A (en) * 2018-05-02 2018-08-21 烟台德迈生物科技有限公司 A kind of device extracting DNA quickly through transfer magnetic bead
CN109706058A (en) * 2019-03-12 2019-05-03 江苏硕世生物科技股份有限公司 Bar magnet magnetosheath fit structure, nucleic acid-extracting apparatus and magnetosheath take method
CN109929757A (en) * 2019-04-30 2019-06-25 广州瑞能医学科技有限公司 A kind of novel nucleic acids extraction apparatus
CN111218398A (en) * 2020-03-25 2020-06-02 广州高盛生物科技股份有限公司 Magnetic bead extraction module and extraction method
CN116731856A (en) * 2023-04-24 2023-09-12 扬州市职业大学(扬州开放大学) High-flux automatic sorting device and sorting method thereof

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CN103897987A (en) * 2014-02-18 2014-07-02 中国农业大学 Automatic extraction device and method of nucleic acid based on nano magnetic beads
CN105543089A (en) * 2016-03-08 2016-05-04 湖南圣湘生物科技有限公司 Magnetic bead based nucleic acid extraction device and method
CN205740995U (en) * 2016-06-01 2016-11-30 杭州沃森生物技术有限公司 A kind of universal type replaceable nucleic acid extraction magnetic frame
CN106367311A (en) * 2016-12-08 2017-02-01 湖南圣湘生物科技有限公司 Nucleic acid extraction system and using method thereof
CN208500910U (en) * 2018-01-22 2019-02-15 无锡百泰克生物技术有限公司 A kind of instrument for extracting nucleic acid

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Publication number Priority date Publication date Assignee Title
CN103881897A (en) * 2013-11-01 2014-06-25 艾康生物技术(杭州)有限公司 Nucleic acid extractor
US20160289665A1 (en) * 2013-11-01 2016-10-06 Leadway (Hk) Limited Nucleic acid extraction apparatus
CN103897987A (en) * 2014-02-18 2014-07-02 中国农业大学 Automatic extraction device and method of nucleic acid based on nano magnetic beads
CN105543089A (en) * 2016-03-08 2016-05-04 湖南圣湘生物科技有限公司 Magnetic bead based nucleic acid extraction device and method
CN205740995U (en) * 2016-06-01 2016-11-30 杭州沃森生物技术有限公司 A kind of universal type replaceable nucleic acid extraction magnetic frame
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108359581A (en) * 2018-05-02 2018-08-03 烟台德迈生物科技有限公司 A kind of device adsorbing mechanical shaking extraction biomolecule by magnetic bead
CN108424838A (en) * 2018-05-02 2018-08-21 烟台德迈生物科技有限公司 A kind of device extracting DNA quickly through transfer magnetic bead
CN109706058A (en) * 2019-03-12 2019-05-03 江苏硕世生物科技股份有限公司 Bar magnet magnetosheath fit structure, nucleic acid-extracting apparatus and magnetosheath take method
CN109929757A (en) * 2019-04-30 2019-06-25 广州瑞能医学科技有限公司 A kind of novel nucleic acids extraction apparatus
CN109929757B (en) * 2019-04-30 2024-03-22 广州瑞能医学科技有限公司 Novel nucleic acid extraction instrument
CN111218398A (en) * 2020-03-25 2020-06-02 广州高盛生物科技股份有限公司 Magnetic bead extraction module and extraction method
CN111218398B (en) * 2020-03-25 2024-02-13 广州高盛生物科技股份有限公司 Magnetic bead extraction module and extraction method
CN116731856A (en) * 2023-04-24 2023-09-12 扬州市职业大学(扬州开放大学) High-flux automatic sorting device and sorting method thereof
CN116731856B (en) * 2023-04-24 2023-12-01 扬州市职业大学(扬州开放大学) High-flux automatic sorting device and sorting method thereof

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