A kind of device extracting DNA quickly through transfer magnetic bead
Technical field
The present invention relates to a kind of devices of extraction DNA, more particularly, to a kind of dress extracting DNA quickly through transfer magnetic bead
It sets.
Background technology
From after being born the DNA double spin model of Watson-Crick, biology has entered a completely new epoch,
Biological educational circles either carries out the detection of pathogenic microorganism, species identification, or carry out the Origin of Species, diversity evaluation and its
The research of affiliation, phyletic evolution etc. is required for isolating and purifying nucleic acid first, therefore makes a living to being extracted into for DNA
All subjects carry out the basis of scientific research in the fields such as object field of medicaments even farming, forestry, husbandary and fishing.And high quality can be extracted
Nucleic acid is the key that then in nucleic acid molecules biological test, and the sensitivity of extracting method, specificity will also be directly related to follow-up
The success or failure of experiment.
Bar magnet method realizes isolating and purifying for nucleic acid by the transfer of magnetic bead, generally by the movement of bar magnet in instrument,
Come realize magnetic bead from sample lysate/combine liquid to cleaning solution, then to eluent transfer, to be automatically performed nucleic acid separation with
The overall process of purifying.Its process and principle include following steps:1) cracking absorption:It is added and waits in the lysate containing magnetic bead
Sample is handled, is sufficiently mixed, lytic cell (appropriate heating contributes to the cracking of cell), the nucleic acid of release is under low ph value with high salt
Specificity is adsorbed onto on magnetic bead, and protein equimolecular is not adsorbed then and stayed in the solution;2) it washs:In the magnetic field of bar magnet
Under effect, magnetic bead is detached with solution, and magnetic bead is transferred in washing buffer by bar magnet, by washing repeatedly, go isolating protein without
The impurity such as machine salt;3) it elutes:After washing, magnetic bead is transferred in elution buffer by bar magnet again, the core under less salt high ph-values
Acid is eluted from magnetic bead, and last bar magnet again removes magnetic bead, that is, completes whole extractions of nucleic acid.
Since magnetic bead itself has higher magnetic responsiveness, it can realize the concentration of magnetic bead by controlling external magnetic field and divide
From this, which allows for whole operation process, becomes simple and fast, and especially for DNA extractive techniques, magnetic bead cooperation is filled with Magneto separate
The equipment set can realize the automation of DNA extractions to the full extent.Currently, paramagnetic particle method DNA extractions are widely used to medicine
In the every field such as diagnosis, agricultural, animal husbandry, judicial expertise.Existing apparatus is complicated for operation, and cost is higher, takes longer, transfer
Sample is easy contaminated during magnetic bead, and can not be completed to shake and be heated, and DNA extraction efficiencies have been seriously affected.
Invention content
A kind of easy to operate present invention aims at providing, the used time is short, and extracting mode is reliable, and it is dirty to can effectively prevent intersection
Dye, the high device that DNA is extracted quickly through transfer magnetic bead of integrated degree.
The technical solution that the present invention solves above-mentioned technical problem is as follows:A kind of dress extracting DNA quickly through transfer magnetic bead
It sets, including support base, extraction head, bar magnet component, bar magnet stock, antifouling board, deep-well plates carrier, deep-well plates fixed block and deep hole
Plate;The support base is arranged below the extraction head and antifouling board, and support base upper surface is equipped with the first Y-direction sliding slot and second
Y-direction sliding slot;Extraction head setting is in support base upper end side, and extraction head and the support base are by X to driving
Band connection, X drive the extraction head along X to reciprocating motion to driving band;The bar magnet component is arranged in the bar magnet stock
Top, bar magnet component are connect with the extraction head by Z-direction sliding rail, bar magnet component moved back and forth along the Z-direction sliding rail and from
The bar magnet stock is stretched into or is removed;The bar magnet stock is connect with the Z-direction sliding rail;The antifouling board passes through the first Y
Band connection is driven to sliding slot and the first Y-direction;In the support base upper end, deep-well plates carrier passes through for deep-well plates carrier setting
Two Y-direction sliding slots and the second Y-direction drive band connection;The deep-well plates fixed block drives the deep-well plates carrier into and out described
Below antifouling board;The deep-well plates are arranged in deep-well plates carrier upper end, and deep-well plates are carried out by the deep-well plates fixed block
It is fixed.
It is preferred as the device for extracting DNA quickly through transfer magnetic bead, the first Y-direction motor is equipped on the inside of the support base,
First Y-direction motor is fixed on support base top plate lower end, and the first Y-direction motor connects first Y-direction and drives band, the first Y-direction motor
Drive band that the antifouling board is driven to move back and forth above support base by first Y-direction.
It is preferred as the device for extracting DNA quickly through transfer magnetic bead, the second Y-direction motor is equipped on the inside of the support base,
Second Y-direction motor is fixed on support base top plate lower end, and the second Y-direction motor connects second Y-direction and drives band, the second Y-direction motor
Drive band that the deep-well plates carrier is driven to move back and forth above support base by second Y-direction.
Preferred as the device for extracting DNA quickly through transfer magnetic bead, the bar magnet component includes magnetic rod fixing frame and magnetic
Stick, bar magnet are uniformly connected to the magnetic rod fixing frame lower end, and bar magnet component is connect by the magnetic rod fixing frame with Z-direction sliding rail.
Preferred as the device for extracting DNA quickly through transfer magnetic bead, the bar magnet component passes through the first synchronous belt locking
Block is connected with the first Z-direction driving band, and first Z-direction driving band connection has the first Z-direction motor, described in the driving of the first Z-direction motor
Bar magnet component is moved along the Z-direction sliding rail.
Preferred as the device for extracting DNA quickly through transfer magnetic bead, the bar magnet stock is equipped with port, the bar magnet
Component is stretched into or is removed from the bar magnet stock by the port.
Preferred as the device for extracting DNA quickly through transfer magnetic bead, the bar magnet stock passes through the second synchronous belt locking
Block is connected with the second Z-direction driving band, and second Z-direction driving band connection has the second Z-direction motor, described in the driving of the second Z-direction motor
Bar magnet stock is moved along the Z-direction sliding rail.
Preferred as the device for extracting DNA quickly through transfer magnetic bead, the antifouling board is in N-shaped, and antifouling board is in transfer magnetic
Pearl is moved in deep-well plates motion process below bar magnet set.
Preferred as the device for extracting DNA quickly through transfer magnetic bead, deep-well plates carrier lower end is equipped with heating component,
Heating component is used to heat the hole position of deep-well plates, and heating component includes heating plate, heat-insulated stone and aluminum heat conducting plate, heating
Piece, heat-insulated stone and aluminum heat conducting plate are overlapped in deep-well plates carrier lower end.
Preferred as the device for extracting DNA quickly through transfer magnetic bead, the bar magnet component and the bar magnet stock use
One row, two row or three row.
The beneficial effects of the invention are as follows:The present invention is by bar magnet set and the cooperation of bar magnet, by magnetic bead from a hole bit transition
To specific hole position, device can be completed to shake, and heat, and can prevent from being contaminated during shifting magnetic bead, deep-well plates can
By manipulator capture while deep-well plates can be also loaded on device.This hair is extracted in medical industry DNA, behaviour
Make simply, the used time is short, and extracting mode is reliable, can effectively prevent cross contamination, and integrated degree is high.
Description of the drawings
Fig. 1 is the device dimensional structure diagram that DNA is extracted quickly through transfer magnetic bead;
Fig. 2 is the device dimensional structure diagram that DNA is extracted quickly through transfer magnetic bead after removing antifouling board;
Fig. 3 is that the device stereochemical structure that DNA is extracted quickly through transfer magnetic bead of display extraction head internal structure is illustrated
Figure;
Fig. 4 is the device dimensional structure diagram that DNA is extracted quickly through transfer magnetic bead under another visual angle;
Fig. 5 is the device internal structure schematic diagram that DNA is extracted quickly through transfer magnetic bead;
Fig. 6 is heating component structural schematic diagram;
Fig. 7 is bar magnet stock, bar magnet nested structure schematic diagram.
Specific implementation mode
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below in conjunction with the accompanying drawings to the present invention
Specific implementation mode be described in detail.Many details are elaborated in the following description in order to fully understand this hair
It is bright.But the invention can be embodied in many other ways as described herein, those skilled in the art can be not
Similar improvement is done in the case of violating intension of the present invention, therefore the present invention is not limited by following public specific embodiment.
Unless otherwise defined, all of technologies and scientific terms used here by the article and belong to the technical field of the present invention
The normally understood meaning of technical staff is identical.Used term is intended merely to description tool in the description of the invention herein
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4 and Fig. 5, a kind of device extracting DNA quickly through transfer magnetic bead, including support
Seat 1, extraction head 2, bar magnet component 3, bar magnet stock 4, antifouling board 5, deep-well plates carrier 6, deep-well plates fixed block 7 and deep-well plates 8;
The support base 1 is arranged below the extraction head 2 and antifouling board 5, and 1 upper surface of support base is equipped with the first Y-direction sliding slot 9 and the
Two Y-direction sliding slots 10;The setting of extraction head 2 passes through in 1 upper end side of the support base, extraction head 2 with the support base 1
X is connected to driving band 11, and X drives the extraction head 2 along X to reciprocating motion to driving band 11;The setting of bar magnet component 3 exists
4 top of the bar magnet stock, bar magnet component 3 are connect with the extraction head 2 by Z-direction sliding rail 12, and bar magnet component 3 is along the Z
It is moved back and forth to sliding rail 12 and stretches into or remove from the bar magnet stock 4;The bar magnet stock 4 is connect with the Z-direction sliding rail 12;
The antifouling board 5 passes through the first Y-direction sliding slot 9 to be connect with the first Y-direction driving band 13;The deep-well plates carrier 6 is arranged in institute
1 upper end of support base is stated, deep-well plates carrier 6 drives band 14 to connect by the second Y-direction sliding slot 10 with the second Y-direction;The deep-well plates are solid
Determining block 7 drives the deep-well plates carrier 6 into and out 5 lower section of the antifouling board;The deep-well plates 8 are arranged in the deep-well plates
6 upper end of carrier, deep-well plates 8 are fixed by the deep-well plates fixed block 7.
In the device one embodiment for extracting DNA quickly through transfer magnetic bead, 1 inside of the support base is equipped with the first Y-direction
Motor 15, the first Y-direction motor 15 are fixed on 1 top plate lower end of support base, and the first Y-direction motor 15 connects the first Y-direction driving band
13, the first Y-direction motor 15 drives band 13 that the antifouling board 5 is driven to move back and forth above support base 1 by first Y-direction.
1 inside of the support base is equipped with the second Y-direction motor 16, and the second Y-direction motor 16 is fixed on 1 top plate lower end of support base, the second Y-direction electricity
Machine 16 connects the second Y-direction driving band 14, and the second Y-direction motor 16 drives band 14 to drive the deep hole by second Y-direction
Onboard frame 6 moves back and forth above support base 1.
In the device one embodiment for extracting DNA quickly through transfer magnetic bead, the bar magnet component 3 includes magnetic rod fixing frame
301 and bar magnet 302, bar magnet 302 be uniformly connected to 301 lower end of the magnetic rod fixing frame, bar magnet component 3 is fixed by the bar magnet
Frame 301 is connect with Z-direction sliding rail 12.The bar magnet component 3 is connected with the first Z-direction by the first synchronous belt locking block 17 and drives band
18, the first Z-direction driving band 18 is connected with the first Z-direction motor 19, and the first Z-direction motor 19 drives the bar magnet component 3 along institute
State the movement of Z-direction sliding rail 12.
In the device one embodiment for extracting DNA quickly through transfer magnetic bead, the bar magnet stock 4 is equipped with port 20,
The bar magnet component 3 is stretched into or is removed from the bar magnet stock 4 by the port 20.The bar magnet stock 4 is same by second
Step locking block 27 is connected with the second Z-direction driving band 21, and the second Z-direction driving band 21 is connected with the second Z-direction motor 22, and second
Z-direction motor 22 drives the bar magnet stock 4 to be moved along the Z-direction sliding rail 12.
In the device one embodiment for extracting DNA quickly through transfer magnetic bead, the antifouling board 5 is in N-shaped, and antifouling board 5 exists
Transfer magnetic bead is moved in 8 motion process of deep-well plates below bar magnet set 23.
Referring to Fig. 6, in device one embodiment that DNA is extracted quickly through transfer magnetic bead, 6 lower end of deep-well plates carrier
Equipped with heating component, heating component is used to heat the hole position of deep-well plates 8, and heating component includes heating plate 24, heat-insulated stone
25 and aluminum heat conducting plate 26, heating plate 24, heat-insulated stone 25 and aluminum heat conducting plate 26 overlap in 6 lower end of deep-well plates carrier.
In the device one embodiment for extracting DNA quickly through transfer magnetic bead, the bar magnet component 3 and the bar magnet stock
4 using a row, two row or three row.
Referring to Fig. 7, a row and two row or other row may be used in bar magnet stock 4 and bar magnet component 3 of the present invention, according to examination
The method of agent is determined, and bar magnet set 23 is in advance put into deep-well plates 8, and deep-well plates 8 are moved to bar magnet set with deep-well plates carrier 6
4 position of frame, bar magnet stock 4 move down, and bar magnet is covered and is clamped.After magnetic bead and reagent is added in deep-well plates 8, the second Z-direction motor 22 passes through
Second Z-direction driving band 21 drives bar magnet stock 4 to be moved along Z-direction sliding rail 12,4 up and down motion mixing of bar magnet stock, bar magnet 302 and magnetic
Stick set 23 moves further to play rising after picking up magnetic bead downwardly together, and magnetic bead is transferred to next position of deep-well plates 8, bar magnet
302 move up, and about 23 concussion of bar magnet set, magnetic bead is cleaned in deep-well plates 8.Antifouling board 5 is in transfer magnetic bead or in deep-well plates 8
It is moved to 23 lower section of bar magnet set when motion process, prevents magnetic bead or liquid from dripping, pollutes.In deep-well plates carrier 6
Heating component is set, corresponding hole position can be heated according to the needs of reagent.Deep-well plates 8 by deep-well plates fixed block 7 from
Dynamic to fix, deep-well plates fixed block 7 can be moved along Y-direction, and deep-well plates 8 are discharged when deep-well plates carrier 6 is moved, when
The movement of deep-well plates carrier 6 locked deep-well plates 8 when entering.Extracting mode of the present invention is reliable, can effectively prevent cross contamination, and one
Body degree is high, improves the efficiency that DNA is extracted by shifting magnetic bead.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.