CN208898912U - A kind of transfer liquid formula nucleic acid-extracting apparatus - Google Patents
A kind of transfer liquid formula nucleic acid-extracting apparatus Download PDFInfo
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- CN208898912U CN208898912U CN201821319534.0U CN201821319534U CN208898912U CN 208898912 U CN208898912 U CN 208898912U CN 201821319534 U CN201821319534 U CN 201821319534U CN 208898912 U CN208898912 U CN 208898912U
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Abstract
A kind of transfer liquid formula nucleic acid-extracting apparatus, frame mid portion is connected with penetrating orbit, crossbeam arm is connected on penetrating orbit, the side of crossbeam arm is connected with clamping device, the other side of crossbeam arm is connected with sample charging mechanism, and clamping device carries out Y-direction along crossbeam arm and moves, and it is mobile that clamping device itself carries out Z-direction, sample charging mechanism carries out Y-direction along crossbeam arm and moves, and it is mobile that sample charging mechanism itself carries out Z-direction;Semiconductor chilling plate is equipped with inside reagent carrier and PCR refrigeration mechanism, it includes concussion motor that deep-well plates, which shake heating mechanism, concussion motor is connected with vibration eccentric wheel, vibration eccentric wheel upper end is connected with heated aluminum block, heated aluminum block upper end is connected with heating top plate, heated aluminum block lower end is connected with damper, and vibration heating baffle is connected on the outside of heated aluminum block and heating top plate.The utility model realizes continuous automatic sample, reagent adding, transfer, incubation and extraction movement, can the product to reagent or extraction carry out the suction nozzle disinfection after refrigeration and use, extraction efficiency is high.
Description
Technical field
The utility model relates to a kind of transfer liquid formula nucleic acid-extracting apparatus, are related to nucleic acid extraction technical field.
Background technique
From after the DNA double spin model birth of Watson-Crick, biology has entered a completely new epoch,
Biological educational circles, either the progress detection of pathogenic microorganism, species identification, or carry out the Origin of Species, diversity evaluation and its
The research of affiliation, phyletic evolution etc. requires to isolate and purify nucleic acid first, therefore makes a living to being extracted into for DNA
All subjects carry out the basis of scientific research in the fields such as object field of medicaments even farming, forestry, husbandary and fishing.And high quality can be extracted
Nucleic acid is the key that then in nucleic acid molecules biological test, and the sensitivity of extracting method, specificity will also be directly related to subsequent
The success or failure of test.
Bar magnet method realizes isolating and purifying for nucleic acid by the transfer of magnetic bead, the general movement by bar magnet in instrument,
Come realize magnetic bead from sample lysate/combine liquid to cleaning solution, then arrive eluent transfer, thus be automatically performed nucleic acid separation with
The overall process of purifying.Existing nucleic acid extraction technical solution, can not achieve automatic continuous sample-adding, reagent adding, incubation and extraction
Etc. processes, cannot achieve the disinfection to suction nozzle, it usually needs carry out nucleic acid extraction between multiple isolated equipment, it is whole to extract
Low efficiency cannot freeze to reagent or the product of extraction in extraction process, and the DNA of extraction is easily broken off, and influence to examine effect
Fruit.
Utility model content
The utility model is based in view of the deficienciess of the prior art, provide a kind of transfer liquid formula nucleic acid-extracting apparatus
Existing automated control technology can be realized continuous automatic sample, reagent adding, transfer, incubation and extraction movement, extraction process
In can not only freeze to reagent or the product of extraction, while being also able to achieve to the suction nozzle disinfection after using, extraction efficiency is high.
The technical solution that the utility model solves above-mentioned technical problem is as follows: a kind of transfer liquid formula nucleic acid-extracting apparatus,
The extraction element includes frame, and the frame bottom end is connected with pedestal, and pedestal is equipped with barcode scanning mechanism, and the frame mid portion connects
It is connected to penetrating orbit, crossbeam arm is connected on the penetrating orbit, the side of the crossbeam arm is connected with clamping device, crossbeam arm
Other side be connected with sample charging mechanism, the clamping device carries out Y-direction along the crossbeam arm and moves, and clamping device itself carries out
Z-direction is mobile, and the sample charging mechanism carries out Y-direction along the crossbeam arm and moves, and it is mobile that sample charging mechanism itself carries out Z-direction;The pedestal
Upper surface be equipped with test tube carrier, suction nozzle carrier, wash needle carrier, run-bake-needle fault carrier, reagent carrier, PCR refrigeration mechanism, PCR lid carry
Frame, deep-well plates carrier, deep-well plates vibration heating mechanism and magnetic force extraction mechanism, inside the reagent carrier and PCR refrigeration mechanism
Equipped with semiconductor chilling plate, the deep-well plates vibration heating mechanism includes concussion motor, and it is inclined that the concussion motor is connected with vibration
Heart wheel, the vibration eccentric wheel upper end are connected with heated aluminum block, and the heated aluminum block upper end is connected with heating top plate, heated aluminum block
Lower end is connected with damper, and vibration heating baffle is connected on the outside of heated aluminum block and heating top plate.
As the preferred embodiment of transfer liquid formula nucleic acid-extracting apparatus, the frame mid portion is connected with fixed cross beam, described
Fixed cross beam and the penetrating orbit are in hollow distribution on said frame, are connected with ultraviolet germicidal on the inside of fixed cross beam.
Fixed cross beam connects the structural strength that frame improves frame, while the ultraviolet germicidal on the inside of fixed cross beam with common group of penetrating orbit
Sterilizing can be carried out for nucleic acid-extracting apparatus.
As the preferred embodiment of transfer liquid formula nucleic acid-extracting apparatus, crossbeam arm upper end is connected with the first Y-direction sliding rail,
Sample charging mechanism is connect by the first Y-direction sliding rail with the crossbeam arm, and sample charging mechanism is connected with sample-adding Y-direction driving electricity by synchronous belt
Machine;Crossbeam arm upper end is connected with the second Y-direction sliding rail, and the second Y-direction sliding rail and the first Y-direction sliding rail are arranged in parallel, clamping device
It is connect by the second Y-direction sliding rail with the crossbeam arm, clamping device is connected with clamping Y-direction driving motor by synchronous belt.Clamp Y
Second Y-direction sliding rail of the clamping device along crossbeam arm upper end is driven to move by synchronous belt to driving motor.
As the preferred embodiment of transfer liquid formula nucleic acid-extracting apparatus, the sample charging mechanism includes sample-adding Z-direction driving motor,
The sample-adding Z-direction driving motor is connected with sample-adding Z-direction driving screw rod, and sample-adding Z-direction driving screw rod is connected with injection linkage seat, injects
Linkage seat is connected with sample-adding pump through being loaded cylinder body, and the sample-adding pump is connected with sample needle.It is loaded Z-direction driving motor and passes through sample-adding Z
It moves up and down to drive screw drives injection linkage seat, injection linkage seat drives sample-adding cylinder body to realize sample needle by sample-adding pump
Sample-adding.
As the preferred embodiment of transfer liquid formula nucleic acid-extracting apparatus, the clamping device includes clamping Z-direction motor, described
Clamping Z-direction motor is connected with clamping Z-direction screw rod, and pinboard, the pinboard lower end connection are arranged on the clamping Z-direction screw rod
There is movable base plate, is connected with crawl fixing seat below the movable base plate, the crawl fixing seat upper end is connected with crawl motor,
The crawl motor is connected with T-type screw rod by synchronous belt, and finger attachment base is arranged on the T-type screw rod, and the finger connects
Joint chair is connected with holding finger.It clamps Z-direction motor and pinboard is driven by clamping Z-direction screw rod, pinboard drives on movable base plate
Lower movement, crawl motor drives the finger attachment base on T-type screw rod to move by synchronous belt, to realize the opening of holding finger
With the completion of closed action.
As the preferred embodiment of transfer liquid formula nucleic acid-extracting apparatus, the needle carrier of washing includes washing needle top plate, washes needle top
It is formed on plate and washes needle liquid bath;The run-bake-needle fault carrier includes run-bake-needle fault baffle, and run-bake-needle fault baffle interior is formed with disinfection trough, the disinfection
Slot upper end is connected with run-bake-needle fault cover board, is formed with run-bake-needle fault slot on the run-bake-needle fault cover board.
As the preferred embodiment of transfer liquid formula nucleic acid-extracting apparatus, the magnetic force extraction mechanism includes magnet lifting electricity
Machine, magnet lifting motor upper end are connected with magnetic base, and magnetic base upper end is connected with magnetic frame supporting plate through being oriented to optical axis, are oriented to
Optical axis passes through the magnetic frame supporting plate and is connected with magnet base top plate, and the motor shaft of magnet lifting motor passes through the magnetic base
It is connect with magnetic frame supporting plate with the magnet base top plate, magnet base top plate two sides are formed with magnet port, magnetic frame supporting plate
Upper end two sides are connected with magnetic elevating plate, and magnetic elevating intralamellar part is embedded with magnetic force body, and magnetic elevating strip moves the magnetic force body
In and out of the magnet port, the external side of magnetic force is connected with briquetting.Magnet lifting motor drives magnet base by motor shaft
Top plate moves up and down, and the magnetic force body for being embedded in magnetic elevating intralamellar part enters and leaves from the magnet port of magnet base top plate two sides, thus
It realizes and the sample being placed on magnet base top plate is extracted by magnetic force body.
As the preferred embodiment of transfer liquid formula nucleic acid-extracting apparatus, the magnetic base upper end-face edge is connected with magnetic frame
Baffle, the magnetic frame supporting plate and magnet base top plate are located on the inside of the magnetic frame baffle;Connection on the inside of the magnetic frame baffle
There is reseting shrapnel.Magnetic frame baffle plays limit barrier effect to the deep-well plates being placed on magnet base top plate, while resetting bullet
Piece can play clamping action.
The utility model relates to transfer liquid formula method for extracting nucleic acid the following steps are included:
Step 1: sample to be extracted is put into test tube carrier, by sweeping during test tube carrier push-in extraction element
Ink recorder structure is scanned record;
Step 2: clamping device, which grabs the deep-well plates being previously placed in deep-well plates carrier, shakes heating mechanism into deep-well plates
Interior, sample charging mechanism is moved to suction nozzle carrier and loads suction nozzle, then is moved at test tube carrier and draws sample, is then added to sample
It is placed in the first round of deep-well plates in deep-well plates vibration heating mechanism;
Step 3: sample charging mechanism, which is moved at reagent carrier, draws reagent, then the reagent drawn is added to and is placed on deep hole
Plate shakes in the first round of deep-well plates in heating mechanism, shakes heating mechanism vibration to addition sample and reagent by deep-well plates
Deep-well plates shaken and heated;
Step 4: after the completion of vibration heating, by deep-well plates pick-and-place to magnetic force extraction mechanism, deep-well plates are put into clamping device
Afterwards, the magnetic force body of magnetic force extraction mechanism rises, and the magnetic bead in deep-well plates is sucked, after sample charging mechanism siphons away liquid, magnetic force body
Deep-well plates are then transferred in deep-well plates vibration heating mechanism by decline, clamping device;
Step 5: after deep-well plates are put into deep-well plates vibration heating mechanism, clamping device continuously adds the reagent of needs, simultaneously
Deep-well plates shake heating mechanism concussion heating, and repeat the above steps three and step 4, until experimental procedure is completed;
Step 6: sample charging mechanism is added to the DNA completed is extracted in PCR refrigeration mechanism, and completes to extract.
As the preferred embodiment of transfer liquid formula method for extracting nucleic acid, in the extracting method, after the completion of extraction, it is loaded machine
Structure, which is moved to, to be washed the needle liquid bath of washing of needle carrier and carries out suction nozzle and wash needle disinfection, and suction nozzle after disinfection is de- by the run-bake-needle fault slot of run-bake-needle fault carrier
Needle.
The utility model frame mid portion is connected with penetrating orbit, and crossbeam arm, the side of crossbeam arm are connected on penetrating orbit
It is connected with clamping device, the other side of crossbeam arm is connected with sample charging mechanism, and clamping device carries out Y-direction along crossbeam arm and moves, presss from both sides
It holds mechanism itself and carries out Z-direction movement, sample charging mechanism carries out Y-direction along crossbeam arm and moves, and it is mobile that sample charging mechanism itself carries out Z-direction;Base
The upper surface of seat is equipped with test tube carrier, suction nozzle carrier, washes needle carrier, run-bake-needle fault carrier, reagent carrier, PCR refrigeration mechanism, PCR lid
Carrier, deep-well plates carrier, deep-well plates shake heating mechanism and magnetic force extraction mechanism, set inside reagent carrier and PCR refrigeration mechanism
There is semiconductor chilling plate, it includes concussion motor that deep-well plates, which shake heating mechanism, and concussion motor is connected with vibration eccentric wheel, and vibration is inclined
Heart wheel upper end is connected with heated aluminum block, and heated aluminum block upper end is connected with heating top plate, and heated aluminum block lower end is connected with damper, adds
Vibration heating baffle is connected on the outside of hot aluminium block and heating top plate.The utility model is based on existing automated control technology, energy
Enough realize continuous automatic sample, reagent adding, transfer, incubation and extraction movement, it cannot be to the production of reagent or extraction in extraction process
Object freezes, while being also able to achieve to the suction nozzle disinfection after using, and extraction efficiency is high.
Detailed description of the invention
Fig. 1 is transfer liquid formula nucleic acid-extracting apparatus structural schematic diagram in embodiment;
Fig. 2 is transfer liquid formula nucleic acid-extracting apparatus schematic diagram of internal structure in embodiment;
Fig. 3 is the structural schematic diagram of pedestal upper surface in embodiment;
Fig. 4 is the pedestal upper end surface structure schematic diagram in embodiment under another visual angle;
Fig. 5 is embodiment middle cross beam arm and sample charging mechanism schematic diagram;
Fig. 6 is embodiment middle cross beam arm and clamping device schematic diagram;
Fig. 7 is PCR refrigeration mechanism schematic diagram in embodiment;
Fig. 8 is magnetic force extraction mechanism schematic diagram in embodiment;
Fig. 9 is that embodiment medium-length hole plate shakes heating mechanism schematic diagram;
Figure 10 is to wash needle carrier schematic diagram in embodiment;
Figure 11 is run-bake-needle fault carrier schematic diagram in embodiment;
Figure 12 is transfer liquid formula method for extracting nucleic acid flow chart;
Wherein, 1, frame;2, pedestal;3, barcode scanning mechanism;4, penetrating orbit;5, crossbeam arm;6, clamping device;7, it is loaded machine
Structure;8, test tube carrier;9, suction nozzle carrier;10, needle carrier is washed;11, run-bake-needle fault carrier;12, reagent carrier;13, PCR refrigeration mechanism;
14, PCR lid carrier;15, deep-well plates carrier;16, deep-well plates shake heating mechanism;17, extraction mechanism;18, semiconductor chilling plate;
19, motor is shaken;20, eccentric wheel is shaken;21, heated aluminum block;22, top plate is heated;23, damper;24, vibration heating baffle;
25, fixed cross beam;26, ultraviolet germicidal;27, the first Y-direction sliding rail;28, it is loaded Y-direction driving motor;29, the second Y-direction sliding rail;
30, Y-direction driving motor is clamped;31, it is loaded Z-direction driving motor;32, sample-adding Z-direction drives screw rod;33, linkage seat is injected;34, add
Sample cylinder body;35, sample-adding pump;36, sample needle;37, Z-direction motor is clamped;38, Z-direction screw rod is clamped;39, pinboard;40, mobile bottom
Plate;41, fixing seat is grabbed;42, motor is grabbed;43, T-type screw rod;44, finger attachment base;45, holding finger;46, needle top is washed
Plate;47, needle liquid bath is washed;48, run-bake-needle fault baffle;49, disinfection trough;50, run-bake-needle fault cover board;51, run-bake-needle fault slot;52, magnet lifting motor;
53, magnetic base;54, it is oriented to optical axis;55, magnetic frame supporting plate;56, magnet base top plate;57, magnet port;58, magnetic elevating
Plate;59, magnetic force body;60, briquetting;61, magnetic frame baffle;62, reseting shrapnel.
Specific embodiment
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, with reference to the accompanying drawing to this
The specific embodiment of utility model is described in detail.Many details are explained in the following description in order to abundant
Understand the utility model.But the utility model can be implemented with being much different from other way described herein, this field
Technical staff can do similar improvement without prejudice to the utility model connotation, therefore the utility model is not by following public affairs
The limitation for the specific embodiment opened.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" packet
Include any and all combinations of one or more related listed items.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7, Fig. 8, Fig. 9, Figure 10 and Figure 11, a kind of transfer liquid formula core
Sour extraction element, the extraction element include frame 1, and 1 bottom end of frame is connected with pedestal 2, and pedestal 2 is equipped with barcode scanning mechanism
3, penetrating orbit 4 is connected in the middle part of the frame 1, and crossbeam arm 5, the side of the crossbeam arm 5 are connected on the penetrating orbit 4
Be connected with clamping device 6, the other side of crossbeam arm 5 is connected with sample charging mechanism 7, the clamping device 6 along the crossbeam arm 5 into
Row Y-direction is mobile, and clamping device 6 itself carries out Z-direction movement, and the sample charging mechanism 7 carries out Y-direction along the crossbeam arm 5 and moves, is loaded
It is mobile that mechanism 7 itself carries out Z-direction;The upper surface of the pedestal 2 is equipped with test tube carrier 8, suction nozzle carrier 9, washes needle carrier 10, run-bake-needle fault
Carrier 11, reagent carrier 12, PCR refrigeration mechanism 13, PCR lid carrier 14, deep-well plates carrier 15, deep-well plates shake heating mechanism 16
With magnetic force extraction mechanism 17, semiconductor chilling plate 18, the deep hole are equipped with inside the reagent carrier 12 and PCR refrigeration mechanism 13
It includes concussion motor 19 that plate, which shakes heating mechanism 16, and the concussion motor 19 is connected with vibration eccentric wheel 20, and the vibration is eccentric
It takes turns 20 upper ends and is connected with heated aluminum block 21,21 upper end of heated aluminum block is connected with heating top plate 22, and 21 lower end of heated aluminum block connects
It is connected to damper 23, vibration heating baffle 24 is connected on the outside of heated aluminum block 21 and heating top plate 22.
Semiconductor chilling plate 18 is also referred to as also thermoelectric module, is a kind of heat pump, without slide unit, applies in some skies
Between be restricted, reliability requirement is high, the occasion of no refrigerant pollution.Using the Peltier effect of semiconductor material, work as direct current
When the galvanic couple that electricity is connected by two kinds of different semiconductor materials, heat can be absorbed respectively at the both ends of galvanic couple and releases heat
The purpose of refrigeration may be implemented in amount.
Referring to Fig. 1, in one embodiment of transfer liquid formula nucleic acid-extracting apparatus, fixation is connected in the middle part of the frame 1
Crossbeam 25, the fixed cross beam 25 and the penetrating orbit 4 are distributed on the frame 1 in hollow, 25 inside of fixed cross beam
It is connected with ultraviolet germicidal 26.Fixed cross beam 25 connects the structural strength that frame 1 improves frame 1 with 4 common groups of penetrating orbit, simultaneously
The ultraviolet germicidal 26 of 25 inside of fixed cross beam can carry out sterilizing for nucleic acid-extracting apparatus.
Referring to Fig. 1, Fig. 5 and Fig. 6, in one embodiment of transfer liquid formula nucleic acid-extracting apparatus, 5 upper end of crossbeam arm
It is connected with the first Y-direction sliding rail 27, sample charging mechanism 7 is connect by the first Y-direction sliding rail 27 with the crossbeam arm 5, and sample charging mechanism 7 passes through
Synchronous belt is connected with sample-adding Y-direction driving motor 28;5 upper end of crossbeam arm is connected with the second Y-direction sliding rail 29, the second Y-direction sliding rail
29 is arranged in parallel with the first Y-direction sliding rail 27, and clamping device 6 is connect by the second Y-direction sliding rail 29 with the crossbeam arm 5, clamping machine
Structure 6 is connected with clamping Y-direction driving motor 30 by synchronous belt.It clamps Y-direction driving motor 30 and clamping device 6 is driven by synchronous belt
The second Y-direction sliding rail 29 along 5 upper end of crossbeam arm moves.
Referring to Fig. 5, in one embodiment of transfer liquid formula nucleic acid-extracting apparatus, the sample charging mechanism 7 includes sample-adding Z-direction
Driving motor 31, the sample-adding Z-direction driving motor 31 are connected with sample-adding Z-direction driving screw rod 32, and sample-adding Z-direction driving screw rod 32 connects
There is injection linkage seat 33, injection linkage seat 33 is connected with sample-adding pump 35 through being loaded cylinder body 34, and the sample-adding pump 35 is connected with sample-adding
Needle 36.It is loaded Z-direction driving motor 31 and drives the driving injection linkage seat 33 of screw rod 32 to move up and down by sample-adding Z-direction, injection linkage
Seat 33 drives sample-adding cylinder body 34 to pass through the sample-adding of the realization sample needle 36 of sample-adding pump 35.
Referring to Fig. 6, in one embodiment of transfer liquid formula nucleic acid-extracting apparatus, the clamping device 6 includes clamping Z-direction
Motor 37, the clamping Z-direction motor 37 are connected with clamping Z-direction screw rod 38, are arranged with pinboard on the clamping Z-direction screw rod 38
39,39 lower end of pinboard is connected with movable base plate 40, and crawl fixing seat 41 is connected with below the movable base plate 40, described
Crawl 41 upper end of fixing seat is connected with crawl motor 42, and the crawl motor 42 is connected with T-type screw rod 43 by synchronous belt, described
Finger attachment base 44 is arranged on T-type screw rod 43, the finger attachment base 44 is connected with holding finger 45.Clamp Z-direction motor 37
Pinboard 39 is driven by clamping Z-direction screw rod 38, pinboard 39 drives movable base plate 40 to move up and down, and crawl motor 42 passes through same
It walks and is moved with the finger attachment base 44 on driving T-type screw rod 43, to realize the complete of the opening and closing movement of holding finger 45
At.
It is described to wash the packet of needle carrier 10 in one embodiment of transfer liquid formula nucleic acid-extracting apparatus referring to Figure 10 and Figure 11
It includes and washes needle top plate 46, wash on needle top plate 46 to be formed with and wash needle liquid bath 47;The run-bake-needle fault carrier 11 includes run-bake-needle fault baffle 48, run-bake-needle fault gear
Disinfection trough 49 is formed with inside plate 48,49 upper end of disinfection trough is connected with run-bake-needle fault cover board 50, is formed on the run-bake-needle fault cover board 50
There is run-bake-needle fault slot 51.
Referring to Fig. 8, in one embodiment of transfer liquid formula nucleic acid-extracting apparatus, the magnetic force extraction mechanism 17 includes magnetic
Iron lifting motor 52,52 upper end of magnet lifting motor are connected with magnetic base 53, and 53 upper end of magnetic base connects through guiding optical axis 54
It is connected to magnetic frame supporting plate 55, guiding optical axis 54 passes through the magnetic frame supporting plate 55 and is connected with magnet base top plate 56, magnet lifting
The motor shaft of motor 52 passes through the magnetic base 53 and magnetic frame supporting plate 55 is connect with the magnet base top plate 56, the magnetic force
56 two sides of seat top plate are formed with magnet port 57, and 55 upper end two sides of magnetic frame supporting plate are connected with magnetic elevating plate 58, magnetic elevating
Plate 58 has been internally embedded magnetic force body 59, and magnetic elevating plate 58 drives the magnetic force body 59 in and out of the magnet port 57, described
Briquetting 60 is connected on the outside of magnetic force body 59.Magnet lifting motor 52 drives magnet base top plate 56 to move up and down by motor shaft, makes
The magnetic force body 59 that magnetic elevating plate 58 is internally embedded enters and leaves from the magnet port 57 of 56 two sides of magnet base top plate, passes through to realize
Magnetic force body 59 extracts the sample being placed on magnet base top plate 56.
In one embodiment of transfer liquid formula nucleic acid-extracting apparatus, 53 upper end-face edge of magnetic base is connected with magnetic force
Frame baffle 61, the magnetic frame supporting plate 55 and magnet base top plate 56 are located at 61 inside of magnetic frame baffle;The magnetic frame gear
Reseting shrapnel 62 is connected on the inside of plate 61.Magnetic frame baffle 61 plays limit resistance to the deep-well plates being placed on magnet base top plate 56
Gear effect, while reseting shrapnel 62 can play clamping action.
It is connected with penetrating orbit 4 in the middle part of the utility model frame 1, crossbeam arm 5 is connected on penetrating orbit 4, crossbeam arm 5
Side is connected with clamping device 6, and the other side of crossbeam arm 5 is connected with sample charging mechanism 7, and clamping device 6 carries out Y along crossbeam arm 5
To movement, clamping device 6 itself carries out that Z-direction is mobile, and sample charging mechanism 7 carries out Y-direction along crossbeam arm 5 and moves, sample charging mechanism 7 itself into
Row Z-direction is mobile;The upper surface of pedestal 2 is equipped with test tube carrier 8, suction nozzle carrier 9, washes needle carrier 10, run-bake-needle fault carrier 11, reagent carrier
12, PCR refrigeration mechanism 13, PCR lid carrier 14, deep-well plates carrier 15, deep-well plates vibration heating mechanism 16 and magnetic force extraction mechanism
17, semiconductor chilling plate 18 is equipped with inside reagent carrier 12 and PCR refrigeration mechanism 13, it includes shake that deep-well plates, which shake heating mechanism 16,
Motor 19 is swung, concussion motor 19 is connected with vibration eccentric wheel 20, and vibration 20 upper end of eccentric wheel is connected with heated aluminum block 21, heats aluminium
21 upper end of block is connected with heating top plate 22, and 21 lower end of heated aluminum block is connected with damper 23, heated aluminum block 21 and heating top plate 22
Outside is connected with vibration heating baffle 24.
The clamping Y-direction driving motor 30 of the utility model drives clamping device 6 along the of 5 upper end of crossbeam arm by synchronous belt
The movement of two Y-direction sliding rails 29, clamping Y-direction driving motor 30 drive clamping device 6 along the 2nd Y of 5 upper end of crossbeam arm by synchronous belt
It is moved to sliding rail 29.It is loaded Z-direction driving motor 31 and drives the driving injection linkage seat 33 of screw rod 32 to move up and down by sample-adding Z-direction,
Inject the sample-adding that linkage seat 33 drives sample-adding cylinder body 34 to realize sample needle 36 by sample-adding pump 35.Clamping Z-direction motor 37 passes through folder
It holds Z-direction screw rod 38 and drives pinboard 39, pinboard 39 drives movable base plate 40 to move up and down, and crawl motor 42 is driven by synchronous belt
Finger attachment base 44 on dynamic T-type screw rod 43 moves, to realize the completion of the opening and closing movement of holding finger 45.Magnetic force
Frame baffle 61 plays limit barrier effect to the deep-well plates being placed on magnet base top plate 56, while reseting shrapnel 62 can play
Clamping action.Magnet lifting motor 52 drives magnet base top plate 56 to move up and down by motor shaft, makes inside magnetic elevating plate 58
The magnetic force body 59 of insertion enters and leaves from the magnet port 57 of 56 two sides of magnet base top plate, to realize through magnetic force body 59 to being placed on
Sample on magnet base top plate 56 extracts.The utility model is based on existing automated control technology, can be realized continuous
Automatic sample, reagent adding, transfer, incubation and extraction act, and cannot freeze to reagent or the product of extraction in extraction process,
It is also able to achieve simultaneously to the suction nozzle disinfection after using, extraction efficiency is high.
Referring to Figure 12, the utility model relates to a kind of transfer liquid formula method for extracting nucleic acid the following steps are included:
S1: sample to be extracted is put into test tube carrier 8, and test tube carrier 8 passes through barcode scanning during being pushed into extraction element
Mechanism 3 is scanned record;
S2: clamping device 6, which grabs the deep-well plates being previously placed in deep-well plates carrier 15, shakes heating mechanism into deep-well plates
In 16, sample charging mechanism 7 is moved to suction nozzle carrier 9 and loads suction nozzle, then is moved at test tube carrier 8 and draws sample, then by sample
It is added in the first round of deep-well plates being placed in deep-well plates vibration heating mechanism 16;
S3: sample charging mechanism 7, which is moved at reagent carrier 12, draws reagent, then the reagent drawn is added to and is placed on deep-well plates
It shakes in the first round of deep-well plates in heating mechanism 16, shakes the vibration of heating mechanism 16 to addition sample and examination by deep-well plates
The deep-well plates of agent are shaken and are heated;
S4: after the completion of vibration heating, by deep-well plates pick-and-place to magnetic force extraction mechanism 17, deep-well plates are put into clamping device 6
Afterwards, the magnetic force body 59 of magnetic force extraction mechanism 17 rises, and the magnetic bead in deep-well plates is sucked, after sample charging mechanism 7 siphons away liquid, magnetic
Power body 59 declines, and deep-well plates are then transferred in deep-well plates vibration heating mechanism 16 by clamping device 6;
S5: after deep-well plates are put into deep-well plates vibration heating mechanism 16, clamping device 6 continuously adds the reagent of needs, simultaneously
Deep-well plates shake the concussion heating of heating mechanism 16, and repeat the above steps three and step 4, until experimental procedure is completed;
S6: sample charging mechanism 7 is added to the DNA completed is extracted in PCR refrigeration mechanism 13, and completes to extract.
In one embodiment of transfer liquid formula method for extracting nucleic acid, in the extracting method, after the completion of extraction, it is loaded machine
Structure 7, which is moved to, to be washed the needle liquid bath 47 of washing of needle carrier 10 and carries out suction nozzle and wash needle disinfection, and suction nozzle after disinfection passes through run-bake-needle fault carrier 11
51 run-bake-needle fault of run-bake-needle fault slot.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it cannot be understood as the limitations to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (8)
1. a kind of transfer liquid formula nucleic acid-extracting apparatus, the extraction element include frame, the frame bottom end is connected with pedestal,
Pedestal is equipped with barcode scanning mechanism, it is characterised in that: the frame mid portion is connected with penetrating orbit, is connected on the penetrating orbit
Crossbeam arm, the side of the crossbeam arm are connected with clamping device, and the other side of crossbeam arm is connected with sample charging mechanism, the clamping
Mechanism carries out Y-direction along the crossbeam arm and moves, and clamping device itself carries out Z-direction movement, and the sample charging mechanism is along the crossbeam arm
It is mobile to carry out Y-direction, it is mobile that sample charging mechanism itself carries out Z-direction;The upper surface of the pedestal is equipped with test tube carrier, suction nozzle carrier, washes
Needle carrier, run-bake-needle fault carrier, reagent carrier, PCR refrigeration mechanism, PCR lid carrier, deep-well plates carrier, deep-well plates shake heating mechanism
With magnetic force extraction mechanism, semiconductor chilling plate is equipped with inside the reagent carrier and PCR refrigeration mechanism, the deep-well plates vibration adds
Heat engine structure includes concussion motor, and the concussion motor is connected with vibration eccentric wheel, and the vibration eccentric wheel upper end is connected with heating
Aluminium block, the heated aluminum block upper end are connected with heating top plate, and heated aluminum block lower end is connected with damper, heated aluminum block and heating top
Vibration heating baffle is connected on the outside of plate.
2. a kind of transfer liquid formula nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the frame mid portion connects
It is connected to fixed cross beam, the fixed cross beam and the penetrating orbit are in hollow distribution, fixed cross beam inside on said frame
It is connected with ultraviolet germicidal.
3. a kind of transfer liquid formula nucleic acid-extracting apparatus according to claim 1, it is characterised in that: crossbeam arm upper end
It is connected with the first Y-direction sliding rail, sample charging mechanism is connect by the first Y-direction sliding rail with the crossbeam arm, and sample charging mechanism passes through synchronous belt
It is connected with sample-adding Y-direction driving motor;Crossbeam arm upper end is connected with the second Y-direction sliding rail, and the second Y-direction sliding rail and the first Y-direction are sliding
Rail is arranged in parallel, and clamping device is connect by the second Y-direction sliding rail with the crossbeam arm, and clamping device is connected with folder by synchronous belt
Hold Y-direction driving motor.
4. a kind of transfer liquid formula nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the sample charging mechanism packet
Sample-adding Z-direction driving motor is included, the sample-adding Z-direction driving motor is connected with sample-adding Z-direction driving screw rod, and sample-adding Z-direction driving screw rod connects
It is connected to injection linkage seat, injection linkage seat is connected with sample-adding pump through being loaded cylinder body, and the sample-adding pump is connected with sample needle.
5. a kind of transfer liquid formula nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the clamping device packet
Clamping Z-direction motor is included, the clamping Z-direction motor is connected with clamping Z-direction screw rod, is arranged with pinboard on the clamping Z-direction screw rod,
The pinboard lower end is connected with movable base plate, is connected with crawl fixing seat, the crawl fixing seat below the movable base plate
Upper end is connected with crawl motor, and the crawl motor is connected with T-type screw rod by synchronous belt, is arranged with hand on the T-type screw rod
Refer to that attachment base, the finger attachment base are connected with holding finger.
6. a kind of transfer liquid formula nucleic acid-extracting apparatus according to claim 1, it is characterised in that: described to wash needle carrier packet
It includes and washes needle top plate, wash on needle top plate to be formed with and wash needle liquid bath;The run-bake-needle fault carrier includes run-bake-needle fault baffle, and run-bake-needle fault baffle interior is formed
There is disinfection trough, the disinfection trough upper end is connected with run-bake-needle fault cover board, is formed with run-bake-needle fault slot on the run-bake-needle fault cover board.
7. a kind of transfer liquid formula nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the magnetic force extractor
Structure includes magnet lifting motor, and magnet lifting motor upper end is connected with magnetic base, and magnetic base upper end is connected through guiding optical axis
There is magnetic frame supporting plate, guiding optical axis passes through the magnetic frame supporting plate and is connected with magnet base top plate, the motor of magnet lifting motor
Axis passes through the magnetic base and magnetic frame supporting plate is connect with the magnet base top plate, and magnet base top plate two sides are formed with magnetic
Tie Tong mouth, magnetic frame supporting plate upper end two sides are connected with magnetic elevating plate, and magnetic elevating intralamellar part is embedded with magnetic force body, magnetic elevating
Strip moves the magnetic force body in and out of the magnet port, and the external side of magnetic force is connected with briquetting.
8. a kind of transfer liquid formula nucleic acid-extracting apparatus according to claim 7, it is characterised in that: on the magnetic base
End margin is connected with magnetic frame baffle, and the magnetic frame supporting plate and magnet base top plate are located on the inside of the magnetic frame baffle;It is described
Reseting shrapnel is connected on the inside of magnetic frame baffle.
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CN201821319534.0U CN208898912U (en) | 2018-08-15 | 2018-08-15 | A kind of transfer liquid formula nucleic acid-extracting apparatus |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108998444A (en) * | 2018-08-15 | 2018-12-14 | 烟台艾德康生物科技有限公司 | A kind of transfer liquid formula nucleic acid-extracting apparatus and method |
CN111269820A (en) * | 2020-03-23 | 2020-06-12 | 上海联方生物科技有限责任公司 | Oscillation mechanism of nucleic acid extractor |
-
2018
- 2018-08-15 CN CN201821319534.0U patent/CN208898912U/en active Active
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108998444A (en) * | 2018-08-15 | 2018-12-14 | 烟台艾德康生物科技有限公司 | A kind of transfer liquid formula nucleic acid-extracting apparatus and method |
CN108998444B (en) * | 2018-08-15 | 2023-11-21 | 烟台艾德康生物科技有限公司 | Transfer liquid type nucleic acid extraction device and method |
CN111269820A (en) * | 2020-03-23 | 2020-06-12 | 上海联方生物科技有限责任公司 | Oscillation mechanism of nucleic acid extractor |
CN111269820B (en) * | 2020-03-23 | 2023-08-08 | 上海联方生物科技有限责任公司 | Vibration mechanism of nucleic acid extractor |
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