CN111269820B - Vibration mechanism of nucleic acid extractor - Google Patents

Vibration mechanism of nucleic acid extractor Download PDF

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Publication number
CN111269820B
CN111269820B CN202010207643.9A CN202010207643A CN111269820B CN 111269820 B CN111269820 B CN 111269820B CN 202010207643 A CN202010207643 A CN 202010207643A CN 111269820 B CN111269820 B CN 111269820B
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wall
seat
nucleic acid
rotation
bulb
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CN111269820A (en
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李尚佶
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Shanghai Lianfang Biotechnology Co ltd
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Shanghai Lianfang Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention discloses a vibrating mechanism of a nucleic acid extraction instrument, which comprises a base, wherein a shell is fixed on the outer wall of the top of the base through bolts, a motor frame is arranged in the middle of the inner wall of the shell, a driving assembly is fixed on the inner wall of the motor frame through bolts, the driving assembly comprises a driving motor, a rotating ball head and an electromagnetic vibrating seat, the outer wall of the bottom end of the rotating ball head is sleeved on the outer wall of an output shaft of the driving motor, the outer wall of the rotating ball head is rotatably connected on the inner wall of the electromagnetic vibrating seat, and the output end of the driving assembly is connected with a vibrating disk. According to the invention, the nucleic acid column is vertically inserted into the column hole, the spherical swing seat is arranged in the column hole, so that the nucleic acid column is in a vertical structure, the nucleic acid column can rotate in an arc line in a shape of 8 in cooperation with the rotation of the upper vibration disk, and the electromagnet rolling ball is arranged in the electromagnetic vibration seat, so that the rotation amplitude can be balanced through magnetic adsorption, the rotation amplitude required by different extraction stages and the speed for rotating the vertical nucleic acid column can be changed, and the extraction effect can be improved.

Description

Vibration mechanism of nucleic acid extractor
Technical Field
The invention relates to the technical field of nucleic acid extractors, in particular to a vibrating mechanism of a nucleic acid extractor.
Background
Nucleic acid extractor, which is an instrument for automatically completing the extraction of sample nucleic acid by using matched nucleic acid extracting reagent, is divided into two types: one type is large automated, commonly referred to as an automated liquid workstation; the other type is a small automatic nucleic acid extractor, and the extraction and purification process is automatically completed by using the packaged matched reagent. The large-scale automatic liquid workstation has high equipment cost and high operation cost, is suitable for extracting thousands of specimens of the same type at a time, so that the application is really less; and small-sized automatic instruments, the operation is convenient and more applied because of low instrument and equipment and operation cost.
The existing vibration is that all parts of substances are separated by utilizing centrifugal force through plane rotation, and the rotation speed is high, but once a nucleic acid column is not tight or is unstable in fixation, the situation that the nucleic acid column is easy to spill out occurs, and in the vibration process, the swinging direction of the nucleic acid column is disordered when the swinging direction does not reach a certain speed, so that the separation and the extraction are influenced.
Disclosure of Invention
Based on the technical problems in the background technology, the invention provides a vibrating mechanism of a nucleic acid extractor.
The invention provides a vibrating mechanism of a nucleic acid extraction instrument, which comprises a base, wherein a shell is fixed on the top outer wall of the base through bolts, a motor frame is arranged in the middle of the inner wall of the shell, a driving assembly is fixed on the inner wall of the motor frame through bolts, the driving assembly comprises a driving motor, a rotating ball head and an electromagnetic vibrating seat, the outer wall of the bottom end of the rotating ball head is sleeved on the outer wall of an output shaft of the driving motor, the outer wall of the rotating ball head is rotationally connected on the inner wall of the electromagnetic vibrating seat, the output end of the driving assembly is connected with a vibrating disc, column holes are distributed in the outer edge of the vibrating disc in an equidistant annular mode, a spherical vibrating seat is rotationally connected in the middle of the inner wall of the column holes, a nucleic acid column is inserted into the inner wall of the spherical vibrating seat, a pressing disc is rotationally connected on the outer wall of the nucleic acid column in a clamped mode, a shield is fixed on the outer wall of one side of the shell, a fixing assembly is arranged at the joint of the shell and the outer wall of the shield, a working indicator lamp is arranged on one side of the shell, which is close to the fixing assembly, and a control panel is arranged.
As a further scheme of the invention, the fixing component comprises a buckle and a clamping seat, the buckle is arranged on one side outer wall of the protective cover, and the outer wall of the clamping seat is integrally connected to the middle part of the outer edge of the shell.
As a further scheme of the invention, the spherical swing seat is of a spherical structure with a through groove in the middle, and the rotation angle between the spherical swing seat and the column hole is 45-90 degrees.
As a further scheme of the invention, the inner wall of the bottom end of the pressure plate is provided with a fixed groove, and the inner wall of the fixed groove is clamped with an ultraviolet disinfection lamp ring.
As a further scheme of the invention, the electromagnetic vibration seat comprises a concave spherical seat, an electromagnet rolling ball and a limiting slide rail, wherein the electromagnet rolling ball is distributed in the middle of the inner wall of the concave spherical seat in an equidistant annular mode, and the limiting slide rail is fixed on the outer edge of the bottom end of the concave spherical seat through a bolt.
As a further scheme of the invention, the rotating ball head comprises a ball head and a magnetic rotating ring, the magnetic rotating ring is arranged on the middle part of the outer wall of the ball head, and the rotating ball head is rotationally connected to the inner wall of the concave spherical seat through the magnetic rotating ring.
As a further scheme of the invention, the driving motor, the ultraviolet disinfection lamp ring and the electromagnetic oscillation seat are all connected with a switch, and the switch is connected with a PLC controller through a wire.
The beneficial effects of the invention are as follows:
1. the oscillating mechanism of the nucleic acid extractor is of an inclined 45-degree structure, the nucleic acid column is vertically inserted into the column hole, a spherical oscillating seat is arranged in the column hole, so that the nucleic acid column is of a vertical structure, and then the nucleic acid column can rotate in an 8-shaped arc line by being matched with the rotation of the oscillating disc, so that the extract and the substance to be extracted in the nucleic acid column are layered, and the inefficiency caused by the problem of unstable rotation is reduced;
2. in this nucleic acid extraction appearance vibration mechanism's drive assembly, be equipped with electromagnetism and vibrate seat and rotate the bulb, through rotating the spherical swing between bulb and the electromagnetism vibration seat, make the vibration dish be conical surface structure rotation, be equipped with the electro-magnet spin in the electromagnetism vibration seat simultaneously, the balanced rotation range of accessible magnetic adsorption changes the required rotation range in different extraction stages and rotates the speed of perpendicular nucleic acid column, promotes the extraction effect.
Drawings
FIG. 1 is a schematic side view of an oscillating mechanism of a nucleic acid extractor according to the present invention;
FIG. 2 is a schematic diagram showing a front view of an oscillating mechanism of a nucleic acid extractor according to the present invention;
fig. 3 is a schematic diagram of a driving assembly of an oscillating mechanism of a nucleic acid extractor according to the present invention.
In the figure: 1 base, 2 control panel, 3 casing, 4 work pilot lamp, 5 guard shield, 6 nucleic acid post, 7 shake dish, 8 fixed subassembly, 9 pressure disk, 10 ball-type swing seat, 11 post holes, 12 electromagnetism shake seat, 13 rotation bulb, 14 driving motor.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Referring to fig. 1-3, a vibration mechanism of a nucleic acid extraction instrument comprises a base 1, a casing 3 is fixed on the top outer wall of the base 1 through bolts, a motor frame is arranged in the middle of the inner wall of the casing 3, a driving component is fixed on the inner wall of the motor frame through bolts, the driving component comprises a driving motor 14, a rotating ball head 13 and an electromagnetic vibration seat 12, the bottom outer wall of the rotating ball head 13 is sleeved on the outer wall of an output shaft of the driving motor 14, the outer wall of the rotating ball head 13 is rotationally connected on the inner wall of the electromagnetic vibration seat 12, the output end of the driving component is connected with a vibration disk 7, column holes 11 are distributed in annular years at equal intervals outside the vibration disk 7, a spherical vibration seat 10 is rotationally connected in the middle of the inner wall of the column holes 11, nucleic acid columns 6 are spliced in the inner wall of the spherical vibration seat 10, a pressure disk 9 is rotationally connected in the middle of the top outer wall of the vibration disk 7, the outer wall of the bottom end of the pressure plate 9 is clamped on the outer wall of the nucleic acid column 6, a protecting cover 5 is fixed on the outer wall of one side of the shell 3, which is close to the oscillating disc 7, through a hinge, a fixing component 8 is arranged at the joint of the shell 3 and the protecting cover 5, a working indicator 4 is arranged on the outer wall of one side of the shell 3, which is close to the fixing component 8, a control panel 2 is arranged on one side of the shell 3, which is close to the working indicator 4, the fixing component 8 comprises a buckle and a clamping seat, the buckle is arranged on the outer wall of one side of the protecting cover 5, the outer wall of the clamping seat is integrally connected on the middle part of the outer side of the shell 3, the spherical oscillating seat 10 is of a spherical structure with a through groove in the middle part, the rotation angle between the spherical oscillating seat 10 and a column hole 11 is 45 DEG to 90 DEG, a fixing groove is arranged on the inner wall of the bottom end of the pressure plate 9, an ultraviolet disinfection lamp ring is clamped on the inner wall of the fixing groove, the electromagnetic oscillating seat 12 comprises a concave spherical seat, an electromagnet rolling ball and a limiting slide rail, and the electromagnet rolling balls are distributed in the middle of the inner wall of the concave spherical seat in an annular mode at equal intervals, the limiting sliding rail is fixed on the outer edge of the bottom end of the concave spherical seat through a bolt, the rotary ball head 13 comprises a ball head and a magnetic rotary ring, the magnetic rotary ring is arranged on the middle of the outer wall of the ball head, the rotary ball head 13 is rotationally connected to the inner wall of the concave spherical seat through the magnetic rotary ring, the driving motor 14, the ultraviolet disinfection lamp ring and the electromagnetic vibration seat 12 are all connected with a switch, and the switch is connected with a PLC (programmable logic controller) through wires.
When the vibrating mechanism of the nucleic acid extractor is used, the protecting cover 5 is opened, the nucleic acid column 6 with the extract is inserted into the obliquely arranged vibrating disc 7 at an angle of 45 degrees, the nucleic acid column 6 is vertically inserted into the column hole 11, the spherical swinging seat 10 is arranged in the column hole 11, the nucleic acid column is in a vertical structure, the driving motor 14 works to drive the electromagnetic swinging seat 12 and the rotating ball head 13, the vibrating disc 7 is in a conical surface structure to rotate through the spherical swinging between the rotating ball head 13 and the electromagnetic swinging seat 12, meanwhile, the electromagnet rolling ball is arranged in the electromagnetic swinging seat 12, the magnetic adsorption balance rotation amplitude is utilized, the rotation amplitude required in different extraction stages and the speed for rotating the vertical nucleic acid column are changed, the rotation of the whole vibrating disc 7 can be in an arc line in a shape of 8, the extract in the nucleic acid column and the substance to be extracted are layered, and then the extract is separated.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.

Claims (4)

1. The utility model provides a nucleic acid extraction appearance vibration mechanism, includes base (1), its characterized in that, the top outer wall of base (1) is through bolt fastening has casing (3), the inner wall middle part of casing (3) is equipped with motor frame, and the inner wall of motor frame is through bolt fastening there being drive assembly, drive assembly includes driving motor (14), rotates bulb (13) and electromagnetism and vibrate seat (12), and the bottom outer wall of rotating bulb (13) cup joints on the output shaft outer wall of driving motor (14), the outer wall rotation of rotating bulb (13) is connected on the inner wall of electromagnetism and vibrate seat (12), the output of drive assembly is connected with vibration dish (7), and the outside equidistance annular distribution of vibration dish (7) has post hole (11), the inner wall middle part rotation of post hole (11) is connected with ball-type swing seat (10), and the inner wall grafting of ball-type swing seat (10) has nucleic acid column (6), the top outer wall middle part rotation of vibration dish (7) is connected with (9), and the bottom outer wall joint of rotating bulb (9) is on the outer wall joint of nucleic acid column (6) pressure disk (3), be close to one side of casing (5) is equipped with through vibration dish (7) and fixed part (5), and one side outer wall that casing (3) is close to fixed subassembly (8) is equipped with work pilot lamp (4), one side that casing (3) is close to work pilot lamp (4) is equipped with control panel (2), spherical swing seat (10) are the spherical structure of middle part logical groove, and rotation angle between spherical swing seat (10) and post hole (11) is 45 to 90, electromagnetism is oscillated seat (12) and is included indent ball-type seat, electro-magnet spin and spacing slide rail, and electro-magnet spin equidistance annular distribution indent ball-type seat's inner wall middle part, spacing slide rail passes through the bolt to be fixed on indent ball-type seat's bottom outward side, rotate bulb (13) including bulb and magnetism rotation ring, and magnetism rotation ring is established on the outer wall middle part of bulb, rotate bulb (13) through magnetism rotation ring rotation connection on indent ball-type seat's inner wall.
2. The oscillating mechanism of a nucleic acid extractor according to claim 1, wherein the fixing component (8) comprises a buckle and a clamping seat, the buckle is arranged on one side outer wall of the protecting cover (5), and the outer wall of the clamping seat is integrally connected to the middle part of the outer edge of the casing (3).
3. The oscillation mechanism of a nucleic acid extractor according to claim 1, wherein a fixing groove is formed in the inner wall of the bottom end of the pressure plate (9), and an ultraviolet disinfection lamp ring is clamped on the inner wall of the fixing groove.
4. The oscillation mechanism of a nucleic acid extractor according to claim 1, wherein the driving motor (14), the ultraviolet disinfection lamp ring and the electromagnetic oscillation seat (12) are all connected with a switch, and the switch is connected with a PLC controller through a wire.
CN202010207643.9A 2020-03-23 2020-03-23 Vibration mechanism of nucleic acid extractor Active CN111269820B (en)

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CN111269820B true CN111269820B (en) 2023-08-08

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CN109609345A (en) * 2019-01-31 2019-04-12 上海交通大学 A kind of rotary type micro-fluidic chip for biological sample nucleic acid extraction
CN109677646A (en) * 2019-02-14 2019-04-26 周睿阳 A kind of magnetic suspension support turntable
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