CN116920457A - Protein separation device and method for collecting urine - Google Patents
Protein separation device and method for collecting urine Download PDFInfo
- Publication number
- CN116920457A CN116920457A CN202311170148.5A CN202311170148A CN116920457A CN 116920457 A CN116920457 A CN 116920457A CN 202311170148 A CN202311170148 A CN 202311170148A CN 116920457 A CN116920457 A CN 116920457A
- Authority
- CN
- China
- Prior art keywords
- urine
- urokinase
- separating device
- protein
- wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000002700 urine Anatomy 0.000 title claims abstract description 75
- 238000000926 separation method Methods 0.000 title claims abstract description 45
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 36
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims description 18
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 claims abstract description 65
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 claims abstract description 65
- 229960005356 urokinase Drugs 0.000 claims abstract description 65
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 49
- 239000000741 silica gel Substances 0.000 claims abstract description 45
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 45
- 238000001179 sorption measurement Methods 0.000 claims abstract description 43
- 238000010438 heat treatment Methods 0.000 claims abstract description 30
- 238000003756 stirring Methods 0.000 claims abstract description 28
- 230000001133 acceleration Effects 0.000 claims abstract description 16
- 238000005192 partition Methods 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 28
- 230000005540 biological transmission Effects 0.000 claims description 13
- 230000002779 inactivation Effects 0.000 claims description 8
- 239000003446 ligand Substances 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 150000003384 small molecules Chemical class 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 6
- 238000004062 sedimentation Methods 0.000 claims description 6
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 7
- 235000017491 Bambusa tulda Nutrition 0.000 claims 7
- 241001330002 Bambuseae Species 0.000 claims 7
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 7
- 239000011425 bamboo Substances 0.000 claims 7
- 230000000694 effects Effects 0.000 abstract description 5
- 239000000499 gel Substances 0.000 abstract 2
- 239000000377 silicon dioxide Substances 0.000 abstract 2
- 230000009849 deactivation Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 229920001296 polysiloxane Polymers 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010008132 Cerebral thrombosis Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 201000001429 Intracranial Thrombosis Diseases 0.000 description 1
- 102000013566 Plasminogen Human genes 0.000 description 1
- 108010051456 Plasminogen Proteins 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/02—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor with moving adsorbents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3814—Affinity chromatography of the substrate or co-factor - enzyme type
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
Abstract
The application discloses a protein separation device and a separation method for collecting urine, which belong to the technical field of protein separation, and comprise a separation device main body, wherein an acceleration adsorption part is arranged in the separation device main body, the acceleration adsorption part comprises a main frame body and a partition rod body, the partition rod body is arranged in the main frame body and divides the interior of the main frame body into an upper area and a lower area, a heating net is arranged in the upper area, a plurality of silica gels are arranged in the separation device main body, two different silica gels adsorb urokinase with different molecular weights respectively, the separation device is stopped after stirring for a period of time, the silica gel suspended in the urine can be divided into an upper layer and a lower layer, the upper layer is silica gel for adsorbing urokinase with small molecular weight, the lower layer is silica gel for adsorbing urokinase with large molecular weight, the heating net is electrified to heat the urine at the upper layer, and the activity of the urokinase with small molecular weight at the upper layer is improved after heating.
Description
Technical Field
The application belongs to the technical field of protein separation, and particularly relates to a protein separation device and a protein separation method for collecting urine.
Background
Proteins are important components of our body cells and tissues, and the body requires proteins to repair damaged tissues, create new tissues, promote cell growth and regeneration, while proteins participate in many biochemical reactions including enzyme-catalyzed reactions, which have a regulatory effect on metabolic processes in the body, whereas urokinase, one of the enzymes, is widely used for the treatment of cardiovascular and cerebrovascular diseases such as myocardial infarction and cerebral thrombosis. It is capable of dissolving thrombus in blood, converting it into plasmin by activating plasminogen, thereby degrading fibrin, dissolving thrombus and restoring blood flow, and conventionally extracting urokinase required in urine in the prior art.
In the prior art, for example, chinese patent grant publications are: CN218666017U discloses a urokinase stirring adsorption device, the above-mentioned device discloses that the box is inside to be equipped with the rotation axis, is provided with the (mixing) shaft in the both sides of rotation axis, installs the scraper blade on the (mixing) shaft, and the scraper blade rotates the in-process along with the rotation axis and can keep away from and be close to the inner wall of box, although realized extracting urokinase from urine at the in-process that the device is stirring adsorbed, still has following defect:
although urokinase is extracted from urine in the above patent, urokinase is generally present in two molecular forms, i.e., molecular weights of 36000 and 54000, and urokinase is used as an active substance, so that deactivation of urokinase during urokinase extraction is required, whereas urokinase having a small molecular weight is much less active than urokinase having a large molecular weight, so that deactivation of urokinase having a small molecular weight during urokinase extraction should be avoided.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.
In order to solve the problems, the application adopts the following technical scheme.
The utility model provides a collect protein separation device in urine, including the separator main part, the inside of separator main part is provided with the adsorption acceleration part, the adsorption acceleration part is including main framework and partition body of rod, partition body of rod sets up in the inside of main framework and with the inside separation of main framework for two upper and lower regions, the upper region is provided with the heating net, multiple silica gel has been placed to the inside of separator main part, the silica gel has the affinity ligand to different molecular weight proteins at the interpolation of course of working surface, the adsorption acceleration part rotates the in-process and accelerates the silica gel and distinguish the absorption to the protein of different molecular weight.
Preferably, the top block of separator main part is connected with the separator lid, and the top of separator lid is provided with drive assembly, and drive assembly is including work motor and stirring main shaft, and work motor is located the top of separator lid, and work motor's output transmission is connected with the stirring main shaft, and stirring main shaft's one end extends to the inside of separator main part and is connected with accelerating adsorption component, and work motor drives stirring main shaft and accelerating adsorption component in proper order and rotates.
Preferably, the accelerating adsorption component further comprises a plurality of sleeves, the sleeves are respectively arranged at the middle part of the main frame body and the middle part of the partition rod body, and one end of the stirring main shaft sequentially penetrates through the sleeves to extend to the bottom of the main body of the separation device.
Preferably, the inner wall of the separating device main body is sleeved with a cylinder wall anti-adhesion component, the cylinder wall anti-adhesion component comprises an anti-adhesion cylinder and a lifting ring, the lifting ring is arranged at the top end of the inner wall of the anti-adhesion cylinder, one side of the separating device main body and one side of the cylinder wall anti-adhesion component are provided with a liquid inlet and a through hole, when the cylinder wall anti-adhesion component is completely positioned in the separating device main body, the liquid inlet is attached to the through hole, the inner wall of the anti-adhesion cylinder is provided with a plurality of anti-adhesion grooves, and the separation distances of the anti-adhesion grooves are equal.
Preferably, two angles of the bottom of the main frame body are oblique angles, the bottom of the separating device main body is provided with an anti-sedimentation assembly, the anti-sedimentation assembly comprises an anti-deviation disc body and a flexible ring, the flexible ring is arranged on the inner wall of the anti-deviation disc body, and the section of the anti-deviation disc body is in an inverted trapezoid shape.
Preferably, a urine inlet pipe is fixedly communicated with one side of the separation device main body, a liquid inlet electromagnetic valve is fixedly arranged on one side of the urine inlet pipe, and a liquid outlet pipeline is fixedly communicated with the bottom end of the separation device main body.
Preferably, the bottom fixedly connected with of absorption part is accelerated two body of rod, the bottom of two body of rod and the bottom fixed connection of main framework inner wall, two body of rod and two oblique angles form quadrilateral structure.
Preferably, the bottom of the separating device main body is also fixedly connected with a base, the base comprises three supporting plates, and the three supporting plates are distributed at the bottom of the separating device main body in a triangular shape.
Preferably, the cavity has been seted up on the top of main framework, and power supply unit has been placed to the inside of cavity, and power supply unit is including holding box body and battery, and the battery sets up in the inside that holds the box body, and the battery is circular telegram to the heating net, still including temperature sensor, central processing unit and drain pipe way, temperature sensor sets up on the inner wall of separator main part, and the drain pipe way sets up in the bottom of separator main part, and one side of drain pipe way is provided with out the liquid solenoid valve.
A method for separating protein in collected urine, which is realized by the device, the method comprises the following steps:
s1, a temperature sensor acquires the temperature of urine, and the temperature of the urine is sent to a central processing unit;
s2, marking the initial detection temperature as the initial detection temperature by the central processing unitThe subsequent detection temperature is marked +.>Will->And->In turn is +.>And threshold->The specific comparison steps are as follows;
s201, the CPU willRespectively is +_corresponding to the preset threshold value>And threshold->Comparison analysis, threshold->For the low molecular urokinase inactivation early warning value, threshold +.>For the limit of the inactivation of small molecule urokinase, < >>>/>If->≤/>Generating a first opening instruction, and executing S202; if->≤/>No instruction is generated if +>Generating a second opening instruction, and executing S203;
s202, after receiving a first opening instruction, the power supply component electrifies the heating net, and the heating net heats urine in the separating device main body, if>/>Generating a first closing instruction and a second opening instruction, < >>For the temperature value detected by the temperature sensor in real time, the power supply component receives a first closing instruction to stop electrifying the heating network, and S203 is executed;
s203, the transmission assembly receives a second opening instruction to drive the accelerating adsorption component to rotate, and the accelerating adsorption component stirs urine; if it is≤/>Generating a second closing instruction, and stopping the operation of the transmission component when the transmission component receives the second closing instruction, if +.>≤/>Generating a first opening instruction, and executing S202;
urokinase concentration in any of steps S204, S202 or S203 is below a thresholdAnd when the central processing unit generates a discharge instruction, the liquid outlet electromagnetic valve is opened after receiving the discharge instruction, and the solution is discharged through the liquid outlet pipeline.
Advantageous effects
Compared with the prior art, the application has the beneficial effects that:
(1) Before urine is introduced into the separating device main body, staff pours silica gel into the separating device main body, in the manufacturing process, the silica gel is added with affinity ligands aiming at proteins with different molecular weights on the surface of the silica gel, namely the added silica gel can be divided into two types, one type of silica gel is added with affinity ligands aiming at urokinase with large molecular weight on the surface, the other type of silica gel is added with affinity ligands aiming at urokinase with small molecular weight on the surface, after the silica gel is added, the staff needs to introduce urine into the separating device main body, and the transmission component drives the acceleration adsorption component to rotate in the separating device main body, the two types of silica gel adsorb urokinase with different molecular weights respectively, after a period of stirring, the silica gel suspended in the urine can be divided into an upper layer and a lower layer, the upper layer is the silica gel adsorbing urokinase with small molecular weight, at the moment, the heating network is electrified to heat the upper layer urine, the activity of the upper layer is improved after the upper layer urokinase with small molecular weight is heated, after the upper layer is heated, after a period of heating, the upper layer is heated, stirring is stopped, and finally, the urokinase is obtained after stirring is continued, and the urokinase is filtered.
(2) According to the application, two angles of the bottom are changed into oblique angles, so that the accelerating adsorption component is easy to generate rotational flow in the separation device main body in the process of rotating the separation device main body, so that the combination of silica gel and urokinase deposited at the bottom of the separation device main body can be driven, meanwhile, the cylinder wall anti-adhesion component is arranged in the separation device main body, the inner wall of the cylinder wall anti-adhesion component is smooth, and the silica gel cannot be easily adhered to the inner wall of the cylinder wall anti-adhesion component, thereby being beneficial to the adsorption of urokinase in urine by the silica gel and the cleaning work of a subsequent device.
Drawings
FIG. 1 is a schematic diagram of a separation device;
FIG. 2 is a schematic diagram of the structure of the acceleration adsorption member;
FIG. 3 is a schematic view showing the internal structure of the main body of the separation device;
FIG. 4 is a schematic view of the structure of the stirring main shaft in the sleeve;
FIG. 5 is a schematic diagram of a power supply assembly;
FIG. 6 is a schematic view of the separation device body in a sleeved relationship with the wall anti-attachment member;
FIG. 7 is a schematic view of the internal structure of the anti-sticking barrel;
FIG. 8 is a schematic view of the separation device body with an anti-settling assembly disposed therein;
FIG. 9 is a schematic diagram of the structure of the anti-settling assembly;
FIG. 10 is a schematic view of the separation device body with the overflow restricting member disposed therein;
FIG. 11 is a schematic view of a structure defining an overflow means;
fig. 12 is a side view of the separation device.
The correspondence between the reference numerals and the component names in the drawings is as follows:
10. a separating device body; 11. a base; 12. a locking member; 13. a limit overflow feature; 20. a separating device cover; 30. a transmission assembly; 31. a working motor; 32. a stirring main shaft; 40. accelerating the adsorption component; 41. a main frame; 42. a partition rod body; 43. a sleeve; 44. a heating net; 45. a power supply assembly; 451. a housing case; 452. a battery; 50. a cylinder wall anti-adhesion component; 51. an anti-adhesion cylinder; 511. an anti-adhesion groove; 52. a pull ring is lifted; 60. a urine inlet tube; 61. a liquid inlet electromagnetic valve; 70. an anti-settling assembly; 71. deviation-preventing disc body; 72. a flexible ring; 80. and a liquid outlet pipeline.
Detailed Description
In order that the above-recited objects, features and advantages of the present application will become more readily apparent, a more particular description of the application will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, but the present application may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present application is not limited to the specific embodiments disclosed below.
Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the application. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
As shown in fig. 1, 3 and 12, the present embodiment provides a protein separating device for collecting urine, which comprises a separating device main body 10, wherein a separating device cover 20 is connected to the top end of the separating device main body 10 in a clamping manner, a transmission assembly 30 is arranged at the top end of the separating device cover 20, the transmission assembly 30 comprises a working motor 31 and a stirring main shaft 32, the working motor 31 is positioned at the top end of the separating device cover 20, the output end of the working motor 31 is in transmission connection with the stirring main shaft 32, one end of the stirring main shaft 32 extends into the separating device main body 10 and is connected with an acceleration adsorption component 40, one side of the separating device main body 10 is fixedly communicated with a urine inlet pipe 60, one side of the urine inlet pipe 60 is fixedly provided with a liquid inlet electromagnetic valve 61, the bottom end of the separating device main body 10 is fixedly communicated with a liquid outlet pipe 80, the bottom of the separating device main body 10 is fixedly connected with a base 11, and the three support plates are distributed at the bottom of the separating device main body 10 in a triangular shape;
in this embodiment, the worker needs to open the cover 20 of the separating device first, put silica gel into the main body 10 of the separating device, close the cover 20 of the separating device after the silica gel is put into the separating device, then the liquid inlet solenoid valve 61 is electrified to open, and the target urine is introduced into the main body 10 of the separating device through the urine inlet pipe 60, and in the process of introducing urine, the working motor 31 starts to work, and drives the stirring main shaft 32 and the accelerating adsorption component 40 to rotate in sequence, so that the accelerating adsorption component 40 rotates in the main body 10 of the separating device, the silica gel can be combined with urokinase in the urine rapidly, the urokinase can be adsorbed on the surface of the silica gel, and the base 11 can keep the stability of the main body 10 of the separating device when the separating device works.
As shown in fig. 2, 3 and 4, the accelerating adsorption component 40 includes a main frame 41 and a partition rod 42, the partition rod 42 is disposed in the main frame 41 and divides the interior of the main frame 41 into an upper region and a lower region, the upper region is provided with a heating net 44, the accelerating adsorption component 40 further includes a plurality of sleeves 43, the plurality of sleeves 43 are respectively disposed in the middle of the main frame 41 and the middle of the partition rod 42, one end of the stirring main shaft 32 sequentially passes through the plurality of sleeves 43 to extend to the bottom of the separation device main body 10, a plurality of silica gel are disposed in the separation device main body 10, affinity ligands for proteins with different molecular weights are added on the surface of the silica gel in the processing process, and the accelerating adsorption component 40 accelerates the silica gel to differentially adsorb proteins with different molecular weights in the rotation process;
the inner part of the main frame 41 is divided into an upper area and a lower area by the partition rod body 42, wherein the upper area and the lower area refer to the upper area and the lower area which are shown when the accelerating adsorption component 40 is placed in the separating device main body 10, the middle part of the partition rod body 42, the middle part of the top end of the main frame 41 and the middle part of the bottom end of the main frame 41 are respectively provided with a sleeve 43, the stirring main shaft 32 sequentially passes through the sleeves 43 downwards and finally is rotationally connected with the supporting seat, the stirring main shaft 32 can drive the accelerating adsorption component 40 to rotate in the separating device main body 10 so as to promote the silica gel to adsorb urokinase in urine, then the silica gel in the separating device main body 10 can be divided into two areas, one area is provided with an affinity ligand for the urokinase with a large molecular weight, the other area is provided with an affinity ligand for the urokinase with a small molecular weight, the two different types of the silica gel adsorb the urokinase with different molecular weights respectively, the silica gel is stopped after being stirred for a period of time, the silica gel suspended in urine can be divided into an upper layer and a lower layer, the upper layer is the silica gel for adsorbing the urokinase with the small molecular weight, the silica gel, the lower layer is the silica gel for adsorbing the urokinase with the large molecular weight, the large molecular weight urokinase is heated, the upper layer is heated after the heating the upper layer is heated, the urokinase is heated, and the heating layer is heated after the heating the small urokinase is heated, and the urokinase is heated, so that the final heating is obtained.
As shown in fig. 3, 6 and 7, the inner wall of the separating device body 10 is sleeved with a cylinder wall anti-adhesion component 50, the cylinder wall anti-adhesion component 50 comprises an anti-adhesion cylinder 51 and a lifting ring 52, the lifting ring 52 is arranged at the top end of the inner wall of the anti-adhesion cylinder 51, one side of the separating device body 10 and one side of the cylinder wall anti-adhesion component 50 are provided with a liquid inlet and a through hole, when the cylinder wall anti-adhesion component 50 is completely positioned in the separating device body 10, the liquid inlet is attached to the through hole, the inner wall of the anti-adhesion cylinder 51 is provided with a plurality of anti-adhesion grooves 511, and the plurality of anti-adhesion grooves 511 are equally spaced;
in this example, the anti-adhesion component 50 of the cylinder wall is disposed on the inner wall of the separating device main body 10, the anti-adhesion component 50 of the cylinder wall includes an anti-adhesion cylinder 51 and a pull ring 52, the outer side of the anti-adhesion cylinder 51 and the inner wall of the separating device main body 10 slide, that is, a worker can pull the anti-adhesion cylinder 51 away from the inner wall of the separating device main body 10 through the pull ring 52, the anti-adhesion cylinder 51 can be placed into the separating device main body 10 through the pull ring 52, the accelerating adsorption component 40 rotates in the inner wall of the separating device main body 10, so that urine rotates in the inner wall of the separating device main body 10, the silicone adsorbs urokinase in the process, centrifugal force is generated, the silicone and urokinase can move together towards the inner wall of the separating device main body 10, the anti-adhesion cylinder 51 is made of materials with smooth surfaces, such as glass, plastics, and the like, because of low surface energy of the smooth surfaces, the tendency of adsorption substances, the silicone and the urokinase can be small, the contact area between the anti-adhesion cylinder 51 and the anti-adhesion cylinder 10 is also small, the silicone can be easily adsorbed on the inner wall of the separating device, and the urokinase can be easily adsorbed on the inner wall of the anti-adhesion cylinder 51, and the urokinase can be easily improved, and the urine kinase can be easily adsorbed on the inner wall of the separating device, and the device can be better washed by the urine kinase, and the urine kinase can be easily washed.
As shown in fig. 4 and 5, the top end of the main frame 41 is provided with a cavity, a power supply assembly 45 is placed in the cavity, the power supply assembly 45 comprises a containing box 451 and a battery 452, the battery 452 is arranged in the containing box 451, and the battery 452 is used for electrifying the heating net 44;
the power supply assembly 45 is arranged at a position closer to the heating net 44, so that the power supply assembly 45 in the cavity is convenient to electrify the heating net 44, the heating net 44 can heat urine above the inside of the separation device main body 10, the heating net 44 is not electrified in a wire way from the outside, and the wire is prevented from affecting the rotation of the acceleration adsorption component 40.
Example 2
This example was further modified on the basis of example 1;
as shown in fig. 8 and 9, two corners of the bottom of the main frame 41 are oblique angles, the bottom of the separating device main body 10 is provided with an anti-sedimentation assembly 70, the anti-sedimentation assembly 70 comprises an anti-deviation disc 71 and a flexible ring 72, the flexible ring 72 is arranged on the inner wall of the anti-deviation disc 71, and the cross section of the anti-deviation disc 71 is inverted trapezoid;
in this embodiment, when two corners of the bottom of the main frame 41 are oblique angles, a rotational flow is formed in the separating device main body 10 during the rotation of the main frame 41 in the separating device main body 10, after the urokinase is adsorbed by the silica gel, a certain precipitate is formed in the bottom of the separating device main body 10 due to the increased mass of the silica gel, but the silica gel only adsorbs a certain amount of urine to form the precipitate at this time, and the silica gel does not have a larger adsorption effect, so that the precipitate can be driven to the urine above after the rotational flow is formed in the separating device main body 10, and the adsorption performance of the silica gel can be utilized to the greatest extent, so that the urokinase can be adsorbed in the urine as much as possible, because the silica gel and the urokinase in the separating device main body 10 can move towards the inner wall of the separating device main body 10 due to the centrifugal force, the precipitate can be formed at the edge of the bottom of the separating device main body 10.
The bottom end of the accelerating adsorption component 40 is fixedly connected with two rod bodies, the bottom ends of the two rod bodies are fixedly connected with the bottom of the inner wall of the main frame 41, and the two rod bodies and the two oblique angles form a quadrilateral structure;
the bottom of the acceleration absorbing member 40 is applied with resistance according to the viscosity of water, and is rotated by a certain damping effect, so that a small range of swirling flow is formed inside the separating apparatus body 10.
As shown in fig. 10 and 11, a limiting overflow part 13 is fixedly connected above the inner wall of the separating device main body 10, a positive trapezoid for limiting the cross section of the overflow part 13 is provided, and a plurality of locking parts 12 which are uniformly distributed are arranged at the outer side of the separating device main body 10;
because the accelerating adsorption part 40 rotates in the separating device main body 10, the urine in the separating device main body 10 continuously rotates in the separating device main body 10, when the urine passes through the separating device main body 10 too much, the urine can impact the bottom of the separating device cover 20 along the side wall of the separating device main body 10, so that the bottom of the separating device cover 20 is also full of urine, when the separating device cover 20 is opened by a worker, the urine is easy to be adhered to the clothes of the worker, the overflow limiting part 13 is arranged above the inner wall of the separating device main body 10, and the overflow limiting part 13 is in a shape of being narrow at the upper part and wide at the lower part, then the urine can be blocked by the overflow limiting part 13 to fall back to the lower part in the bottom process of the separating device cover 20, the bottom of the separating device cover 20 is prevented from being full of urine, and the worker can conveniently open the separating device cover 20 to perform subsequent operation or change silica gel after the urokinase extraction work is finished.
Example 3
The embodiment is further improved based on embodiment 1, and further comprises a temperature sensor, a central processing unit and a protein concentration detector, wherein the temperature sensor and the protein concentration detector are arranged on the inner wall of the separating device main body 10, and a liquid outlet electromagnetic valve is arranged on one side of the liquid outlet pipeline 80;
the temperature sensor is used for acquiring the temperature of the urine, sending the urine temperature to the central processing unit, and the central processing unit marks the initial detection temperature asThe subsequent detection temperature is marked +.>Will->And->In turn is +.>And threshold valueThe specific comparison steps are as follows;
step (a)First, the CPU willRespectively is +_corresponding to the preset threshold value>And threshold->Comparison analysis, threshold->For the low molecular urokinase inactivation early warning value, threshold +.>For the limit of the inactivation of small molecule urokinase, < >>>/>If->≤/>Generating a first opening instruction and executing the second step; if->≤/>No instruction is generated if +>Generating a second opening instruction and executing the third step;
step two, after receiving the first opening command, the power supply assembly 45 energizes the heating net 44, and the heating net 44 heats the urine in the separator body 10, if>/>Generating a first closing instruction and a second opening instruction, < >>For the temperature value detected by the temperature sensor in real time, the power supply assembly 45 receives the first closing instruction to stop energizing the heating network 44, and performs the third step;
step three, the transmission assembly 30 receives a second opening instruction to drive the acceleration adsorption component 40 to rotate, and the acceleration adsorption component 40 stirs urine; if it is≤/>Generating a second closing command, wherein the transmission assembly 30 receives the second closing command to stop working, and if +.>≤/>And generating a first opening instruction and executing the second step.
Step four, the urokinase concentration in any one of the step two or the step three is lower than the threshold valueWhen the liquid outlet electromagnetic valve receives the discharge instruction, the liquid outlet electromagnetic valve is opened, and the solution is discharged through the liquid outlet pipeline 80.
Threshold valueFor the low molecular urokinase inactivation early warning value, threshold +.>For the limit of the inactivation of small molecule urokinase, < >>≤/>It indicates that the initial temperature of urine just added to the inside of the separation device body 10 is lower than the limit value of deactivation of small molecule urokinase, and that the urine should be immediately heated to avoid deactivation of small molecule urokinase, < >></>≤/>The initial temperature of the surface urine is between the early warning value of the deactivation of the small molecular urokinase and the limit value of the deactivation of the small molecular urokinase, and the surface urine needs to be automatically cooled to +.>The urine is heated by the heating net 44 and +.>>/>The real-time temperature value in the first stage is higher than the activity-losing early-warning value of small molecule urokinase, at present, urine needs to be stirred, and the urokinase is adsorbed by silica gel;
same reasonAnd->And->In contrast with +.>The same way is used for comparison, but it should be noted that the central processingThe first is through->And->And->Comparing and analyzing, selecting to generate the first opening instruction, the second opening instruction or no instruction, and then generating the first opening instruction, the second opening instruction or no instruction by +.>And->And->Comparison analysis was performed until the urokinase concentration in the solution was below the threshold +.>In this case, a discharge command is generated to discharge the solution from the inside of the separator main body 10.
The foregoing is a further elaboration of the present application in connection with the detailed description, and it is not intended that the application be limited to the specific embodiments shown, but rather that a number of simple deductions or substitutions be made by one of ordinary skill in the art without departing from the spirit of the application, should be considered as falling within the scope of the application as defined in the appended claims.
Claims (12)
1. A protein separation device for collecting urine, comprising a separation device body (10), characterized in that: the inside of separator main part (10) is provided with acceleration adsorption part (40), acceleration adsorption part (40) is including main framework (41) and cut off body of rod (42), cut off body of rod (42) and set up in the inside of main framework (41) and with the inside separation of main framework (41) two upper and lower regions, upper region is provided with heating net (44), multiple silica gel has been placed to the inside of separator main part (10), the silica gel has been added the affinity ligand to different molecular weight proteins in the course of working in the surface, acceleration adsorption part (40) rotatory in-process acceleration silica gel carries out the differential adsorption to the protein of different molecular weight.
2. The protein isolate device for collecting urine as claimed in claim 1, wherein: the top block of separator main part (10) is connected with separator lid (20), the top of separator lid (20) is provided with drive assembly (30), drive assembly (30) are including work motor (31) and stirring main shaft (32), work motor (31) are located the top of separator lid (20), the output transmission of work motor (31) is connected with stirring main shaft (32), the one end of stirring main shaft (32) extends to the inside of separator main part (10) and is connected with accelerating adsorption component (40), work motor (31) drive stirring main shaft (32) and accelerating adsorption component (40) in proper order and rotate.
3. The protein separation device for collecting urine according to claim 2, wherein: the accelerating adsorption component (40) further comprises a plurality of sleeves (43), the sleeves (43) are respectively arranged at the middle part of the main frame body (41) and the middle part of the partition rod body (42), and one end of the stirring main shaft (32) sequentially penetrates through the sleeves (43) to extend to the bottom of the separation device main body (10).
4. The protein isolate device for collecting urine as claimed in claim 1, wherein: the inner wall cover of separator main part (10) is equipped with section of thick bamboo wall anti-adhesion part (50), section of thick bamboo wall anti-adhesion part (50) are including anti-adhesion section of thick bamboo (51) and pull ring (52), pull ring (52) set up in the top of anti-adhesion section of thick bamboo (51) inner wall, one side of separator main part (10) and one side feed liquor hole and the through-hole of section of thick bamboo wall anti-adhesion part (50), when section of thick bamboo wall anti-adhesion part (50) are located the inside of separator main part (10) completely, the feed liquor hole is laminated mutually with the through-hole, a plurality of anti-adhesion grooves (511) have been seted up to the inner wall of anti-adhesion section of thick bamboo (51), a plurality of anti-adhesion grooves (511) are equidistant equal.
5. The protein isolate device for collecting urine as claimed in claim 1, wherein: the two corners of the bottom of the main frame body (41) are oblique angles, an anti-sedimentation assembly (70) is arranged at the bottom of the separation device main body (10), the anti-sedimentation assembly (70) comprises an anti-deviation disc body (71) and a flexible ring (72), the flexible ring (72) is arranged on the inner wall of the anti-deviation disc body (71), and the section of the anti-deviation disc body (71) is in an inverted trapezoid shape.
6. The protein isolate device for collecting urine as claimed in claim 1, wherein: one side of the separating device main body (10) is fixedly communicated with a urine inlet pipe (60), one side of the urine inlet pipe (60) is fixedly provided with a liquid inlet electromagnetic valve (61), and the bottom end of the separating device main body (10) is fixedly communicated with a liquid outlet pipeline (80).
7. The protein isolate device for collecting urine as claimed in claim 5, wherein: the bottom fixedly connected with of accelerating adsorption component (40) two body of rod, the bottom of two body of rod and the bottom fixed connection of main framework (41) inner wall, two body of rod and two oblique angles form quadrilateral structure.
8. The protein separation device for collecting urine according to claim 2, wherein: the bottom of separator main part (10) still fixedly connected with base (11), base (11) include three backup pad, and three backup pad are the triangle-shaped and distribute in the bottom of separator main part (10).
9. The protein separation device for collecting urine according to claim 2, wherein: the cavity has been seted up on the top of main framework (41), and power supply unit (45) have been placed to the inside of cavity, and power supply unit (45) are including holding box body (451) and battery (452), and battery (452) set up in the inside that holds box body (451), and battery (452) are to heating net (44) the circular telegram.
10. The protein isolate device for collecting urine as claimed in claim 9, wherein: the device comprises a separating device body (10), and is characterized by further comprising a temperature sensor, a central processing unit, a liquid outlet pipeline (80) and a protein concentration detector, wherein the temperature sensor is arranged on the inner wall of the separating device body (10), the liquid outlet pipeline (80) is arranged at the bottom end of the separating device body (10), a liquid outlet electromagnetic valve is arranged on one side of the liquid outlet pipeline (80), and the protein concentration detector is arranged on the inner wall of the separating device body (10).
11. A method for separating protein in collected urine, which is characterized in that: implemented with a separation device according to any one of claims 1 to 10, the method comprising at least a data acquisition phase and a separation execution phase:
s1, data acquisition: the temperature sensor acquires the temperature of the urine, and sends the urine temperature to the central processing unit;
s2, separation execution stage: the CPU marks the initial detected temperature asThe subsequent detection temperature is marked +.>Will beAnd->In turn is +.>And threshold->Comparing according to the comparison resultAnd executing a corresponding separation flow.
12. The method for separating protein from urine collected as set forth in claim 11, wherein the separation is performed in a stage ofAnd->In turn is +.>And threshold->The process of performing the comparison further comprises:
s201, the CPU willRespectively is +_corresponding to the preset threshold value>And threshold->Comparison analysis, threshold->For the low molecular urokinase inactivation early warning value, threshold +.>For the limit of the inactivation of small molecule urokinase, < >>>/>If->≤/>Generating a first opening instruction, and executing S202; if->≤/>No instruction is generated if +>Generating a second opening instruction, and executing S203;
s202, after receiving the first opening instruction, the power supply assembly (45) electrifies the heating net (44), and the heating net (44) heats urine in the separating device main body (10), if>/>Generating a first closing instruction and a second opening instruction, < >>For the temperature value detected by the temperature sensor in real time, the power supply assembly (45) receives a first closing instruction to stop energizing the heating network (44), and S203 is executed;
s203, the transmission assembly (30) receives a second opening instruction to drive the accelerating adsorption component (40) to rotate, and the accelerating adsorption component (40) stirs urine; if it is≤/>Generating a second closing instruction and transmittingThe movable component (30) receives a second closing instruction to stop working, if->≤/>Generating a first opening instruction, and executing S202;
urokinase concentration in any of steps S204, S202 or S203 is below a thresholdWhen the liquid outlet electromagnetic valve is used, the central processing unit generates a discharge instruction, the liquid outlet electromagnetic valve is opened after receiving the discharge instruction, and the solution is discharged through the liquid outlet pipeline (80).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311170148.5A CN116920457B (en) | 2023-09-12 | 2023-09-12 | Protein separation device and method for collecting urine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311170148.5A CN116920457B (en) | 2023-09-12 | 2023-09-12 | Protein separation device and method for collecting urine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116920457A true CN116920457A (en) | 2023-10-24 |
| CN116920457B CN116920457B (en) | 2023-11-24 |
Family
ID=88384600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311170148.5A Active CN116920457B (en) | 2023-09-12 | 2023-09-12 | Protein separation device and method for collecting urine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116920457B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117542460A (en) * | 2024-01-09 | 2024-02-09 | 江苏尤里卡生物科技有限公司 | Adaptive parameter optimization method and system for urokinase separation |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205528809U (en) * | 2016-04-16 | 2016-08-31 | 三门县职业中等专业学校 | Full -automatic urokinase preparation equipment |
| CN210367668U (en) * | 2019-05-17 | 2020-04-21 | 宁波瑞萃生物科技有限公司 | Urokinase preparation facilities |
| CN211546556U (en) * | 2019-12-13 | 2020-09-22 | 邢台医学高等专科学校第二附属医院 | Urokinase reaction kettle |
| CN215799605U (en) * | 2021-09-27 | 2022-02-11 | 福州飞净生物科技有限公司 | Trypsin enzymolysis device |
| CN216946956U (en) * | 2021-11-19 | 2022-07-12 | 天津市企德环保科技有限公司 | Vehicle-mounted urine processing equipment |
-
2023
- 2023-09-12 CN CN202311170148.5A patent/CN116920457B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205528809U (en) * | 2016-04-16 | 2016-08-31 | 三门县职业中等专业学校 | Full -automatic urokinase preparation equipment |
| CN210367668U (en) * | 2019-05-17 | 2020-04-21 | 宁波瑞萃生物科技有限公司 | Urokinase preparation facilities |
| CN211546556U (en) * | 2019-12-13 | 2020-09-22 | 邢台医学高等专科学校第二附属医院 | Urokinase reaction kettle |
| CN215799605U (en) * | 2021-09-27 | 2022-02-11 | 福州飞净生物科技有限公司 | Trypsin enzymolysis device |
| CN216946956U (en) * | 2021-11-19 | 2022-07-12 | 天津市企德环保科技有限公司 | Vehicle-mounted urine processing equipment |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117542460A (en) * | 2024-01-09 | 2024-02-09 | 江苏尤里卡生物科技有限公司 | Adaptive parameter optimization method and system for urokinase separation |
| CN117542460B (en) * | 2024-01-09 | 2024-03-22 | 江苏尤里卡生物科技有限公司 | Adaptive parameter optimization method and system for urokinase separation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116920457B (en) | 2023-11-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN116920457B (en) | Protein separation device and method for collecting urine | |
| US5786207A (en) | Tissue dissociating system and method | |
| CN104893967B (en) | Labor-saving automatic plasmid extraction element | |
| NO314715B1 (en) | Apparatus and method for separating a liquid sample | |
| EP0640124B1 (en) | Variable volume reactor-type device and process for the culture of cellular material | |
| CN1582191A (en) | Extractor, chemical analyzer and chemical analyzing method | |
| CN102213655A (en) | Full-automatic microbial staining film production equipment and staining method | |
| CN210023235U (en) | Soil leaching repair tower | |
| JP2710420B2 (en) | Rotary column reactor | |
| CN104148029A (en) | Preparation method and application method of heavy metal ion biological adsorbent | |
| CN212669672U (en) | A little biological separation device for microbiological assay | |
| CN206396221U (en) | The equipment that a kind of high flux fast Acquisition purifies circulating tumor cell | |
| US5013446A (en) | Chromatographic gel contactor and process | |
| CN215992273U (en) | Plant tissue culture rotating tube apparatus disinfection device | |
| CN115477993A (en) | AI intelligent adipose-derived stem cell culture method | |
| US5922211A (en) | Method for centrifugal particle separation, particularly for use in the biological sector | |
| CN206580672U (en) | A kind of unattended countryside sewage treatment equipment of low energy consumption | |
| CN109142019B (en) | Suspended biological experiment sample thawing device | |
| CN212581712U (en) | Fermentation device for domestic sewage treatment | |
| CN114354324A (en) | Automatic cell wax block preparation instrument and method for preparing cell wax block based on same | |
| CN107418871A (en) | A kind of preparation method of self-reacting device for enrichment cycles tumour cell | |
| CN209778851U (en) | Microorganism screening and propagation device | |
| CN110628573B (en) | Potato nucleic acid extraction device and method | |
| CN210193840U (en) | Nucleic acid extraction and purification device | |
| CN207418531U (en) | A kind of segmented sewage disposal detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |