CN104893967B - Labor-saving automatic plasmid extraction element - Google Patents
Labor-saving automatic plasmid extraction element Download PDFInfo
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- CN104893967B CN104893967B CN201510339040.3A CN201510339040A CN104893967B CN 104893967 B CN104893967 B CN 104893967B CN 201510339040 A CN201510339040 A CN 201510339040A CN 104893967 B CN104893967 B CN 104893967B
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- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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Abstract
The present invention relates to a kind of labor-saving automatic plasmid extraction elements, including master controller, Centrifugal vibration device, and it is respectively arranged at the liquid adding and extracting device and lifting gland rotary lifting mechanism of Centrifugal vibration device two sides, centrifugal seat is provided on Centrifugal vibration device, multiple centrifugation tube seats are provided in centrifugal seat, positioning inductor is set by one of centrifugation tube seat, sensor corresponding with positioning inductor is provided in centrifugal seat, going up and down gland rotary lifting mechanism includes rotatable bracket and liftable lifting gland, bracket is L shape, lifting gland is connected on bracket, centrifugation pipe close corresponding with centrifugation tube seat is provided below in lifting gland, liquid adding and extracting device includes rotatable L bracket and the liquid relief disk that is set in L bracket, multiple liquid-feeding tubes and pipette are provided on liquid relief disk, electromagnetism is connected on liquid-feeding tube Valve.The present invention can realize the extraction of plasmid automatically, improve production efficiency, reduce manual operation, and improve the purity of the plasmid of extraction.
Description
It is that November 29, invention and created name in 2013 are that the present invention, which is application No. is the 201310637644.7, applying date,
The divisional application of the application for a patent for invention of " automatic plasmid extraction element ".
Technical field
The present invention relates to Bioexperiment apparatus field, especially a kind of automatic plasmid extraction element.
Background technique
Plasmid be attached to the acellular in cell chromosome or core area DNA it is original can independently replicate it is lesser
DNA molecular, it is that a useful target DNA fragment is sent into recipient cell by recombinant DNA technology and goes to be bred
With the common carrier of expression.Thus plasmid extraction is a routine operation in gene cloning procedures, at present usually using alkali cracking
Solution prepares plasmid from Escherichia coli.Principle are as follows: be denaturalized Plasmid DNA and chromosomal DNA using high ph-values solution, precipitate simultaneously
Protein.PH value is adjusted to neutrality again, Plasmid DNA is smaller, it is easy to which renaturation is at double-strand.And chromosomal DNA is larger, Bu Huifu
Property, entanglement reticulates insoluble matter, so as to be removed by centrifugation.General step is to be centrifuged inoculum to obtain bacterium
Body is added re-suspension liquid, adds the bacteria lysis solution newly prepared, and it is molten to be eventually adding the ice-cold neutralization for neutralizing PH
Liquid is then centrifuged for, removal precipitating, and DNA adsorption column is added in supernatant, and centrifugation removal waste liquid adds washing lotion 1 and washes one time,
Centrifugation removal waste liquid, washing lotion 2 are washed 2 times, and centrifugation removal waste liquid finally obtains pure Plasmid DNA with elution.It is therefrom visible
Complex for operation step, 20 plasmids needs of every extraction are uncapped pass lid up to more than 400 times, are centrifuged more than 10 times.Since plasmid extraction is
Be engaged in the laboratory of molecular biology research daily will routine techniques, thus larger workload, and due to its machinery weight
Renaturation is higher, provides possibility for automation.
Summary of the invention
It is an object of the invention to for existing plasmid extraction process manual operation repeatability described in background technology
The problem of height, heavy workload, provides a kind of labor-saving automatic plasmid extraction element for being able to solve foregoing problems.
The technical solution that can be realized goal of the invention of the invention is as follows:
A kind of labor-saving automatic plasmid extraction element comprising master controller, Centrifugal vibration device and set respectively
It is placed in the liquid adding and extracting device and lifting gland rotary lifting mechanism of Centrifugal vibration device two sides, is provided on Centrifugal vibration device
Centrifugal seat is provided with multiple centrifugation tube seats in centrifugal seat, and positioning inductor is arranged by one of centrifugation tube seat, sets in centrifugal seat
It is equipped with sensor corresponding with positioning inductor, lifting gland rotary lifting mechanism includes rotatable bracket and liftable liter
Decompression lid, bracket are L shape, and lifting gland is connected on bracket, and centrifugation corresponding with centrifugation tube seat is provided below in lifting gland
Pipe close, liquid adding and extracting device include rotatable L bracket and the liquid relief disk that is set in L bracket, are provided on liquid relief disk
Multiple liquid-feeding tubes and pipette connect solenoid valve on liquid-feeding tube.
Above-mentioned Centrifugal vibration device includes pedestal, vibrating motor, vibrating seat and centrifuge.
Setting centrifugation tube seat is even number in above-mentioned centrifugal seat；Lifting gland below be correspondingly arranged on identical quantity from
Heart pipe close；According to the difference of the specific process for extracting DNA plasmid, need to be arranged the centrifugation tube seat of different number.
6 liquid-feeding tubes and two pipette are provided on above-mentioned liquid adding and extracting device；DNA matter is extracted according to specific
The difference of the process of grain, needs to be arranged the liquid-feeding tube and pipette of different number.
It is provided near liquid adding and extracting device for the ease of being focused on to waste liquid, in above-mentioned centrifugal seat useless
Liquid bath.
The working principle of labor-saving automatic plasmid extraction element of the invention are as follows: centrifugation vibration is controlled by main controller
The rotation and vibration, the rotation of liquid adding and extracting device and lifting of dynamic device and to liquid, lifting are injected and taken out in centrifuge tube
The rotation and lifting of gland rotary lifting mechanism, the switch of solenoid valve, be centrifuged pipe close compression and release and centrifugation lid open
It closes.It may be provided with even number centrifugation tube seat in centrifugal seat as needed, in use, centrifuge tube and DNA adsorption column interval are put into
It is centrifuged in tube seat, is illustrated for four centrifugation tube seats are set in centrifugal seat below, four centrifugation tube seats sequentially number and are
No.1 is to No. four；There is positioning inductor by No.1 centrifugation tube seat, it is automatic with energy after corresponding sensor sensing on centrifugal work platform
It is parked on the position of No.1.When extracting plasmid, firstly, manually clean No.1 centrifuge tube and No. three centrifuge tubes are respectively put into
No.1 is centrifuged in tube seat and No. three centrifugation tube seats, and No. two DNA adsorption columns and No. four DNA adsorption columns are respectively put into No. two centrifuge tubes
In slot and four No. three centrifugation tube seats, and by the inoculum of the equal volume fermented (about 1.5mL) be added No.1 and
In No. three centrifuge tubes, then the bracket of rotation lifting gland rotary lifting mechanism, will go up and down side horizontal of the gland from centrifugal seat
The top of centrifugal seat is turned to, then moves down lifting gland, four centrifugation pipe closes being placed in below lifting gland in advance are unclamped
After be pressed on four corresponding centrifuge tubes, then bracket move up and by lifting gland horizontally rotate to original position.Then,
Centrifugation lid is fastened, Centrifugal vibration device is to the inoculum rotating centrifugal in No.1 and No. three centrifuge tubes；After the completion of centrifugation, from
Heart Gai Kaiqi goes up and down gland from the side horizontal of centrifugal seat and turns to the upper surface of centrifugal seat, then moves down four centrifugation pipe closes are same
Shi Songkai is simultaneously fixed on lifting gland in the following, buck covers shifting, then horizontally rotates bracket to original position, and at this moment bacterium is heavy
It forms sediment in the bottom of No.1 and No. three centrifuge tubes, the L bracket of subsequent liquid adding and extracting device rotates, liquid relief disk is horizontally rotated
The upper surface of centrifugal seat, being rotated further by liquid relief disk turns to the first waste liquid suction pipe right above No.1 centrifuge tube, moves down liquid relief disk, by the
The long plastic tube disposably that one waste liquid pipette tip is loaded onto advance slowly enters No.1 centrifuge tube, by supernatant fluid therein
It slowly blots, then rotates above liquid relief disk to waste liquid tank, waste liquid is discharged in waste liquid tank, being rotated further by liquid relief disk makes the first waste liquid
Suction pipe turns to right above No. three centrifuge tubes, moves down liquid relief disk for the long plastic tube of the first waste liquid suction pipe lower end and slowly enters No. three
Supernatant fluid therein is also slowly blotted and is discharged in waste liquid tank by centrifuge tube.Then liquid relief disc spins move the first liquid-feeding tube
Then plus the weight of equal volume it moves right above No.1 centrifuge tube and quantitative 250 μ l of re-suspension liquid is added by solenoid valve control,
Suspension is into No. three centrifuge tubes, and in the way described above, centrifugation pipe close is pressed on centrifuge tube, starts Centrifugal vibration device, centrifugation
Pipe acutely vibrates 1 minute, makes microorganism even suspension, then rotating liquid relief disk makes the second liquid-feeding tube be moved respectively to No.1 centrifugation
The 250 μ l of bacteria lysis solution newly prepared is added above pipe and No. three centrifuge tubes and by solenoid valve control, passes through Centrifugal vibration
Device, which gently vibrates centrifuge tube according to above-mentioned method, mixes solution, and finally rotating liquid relief disk moves third liquid-feeding tube respectively
It is molten that the ice-cold neutralization for neutralizing pH is added above to No.1 centrifuge tube and No. three centrifuge tubes and by solenoid valve control
350 μ l of liquid, by Centrifugal vibration device according to above-mentioned method gently vibrate centrifuge tube make solution mix after be centrifuged.Centrifugation terminates
Afterwards, the long plastic tube disposably that mobile and rotation liquid relief disk loads onto the second imbibition pipe end in advance extends to No.1 centrifuge tube
The middle supernatant fluid by wherein containing Plasmid DNA is slowly sucked out, and Plasmid DNA will be contained in the second pipette by being rotated further by liquid relief disk
Supernatant fluid be slowly added in No. two DNA adsorption columns, it is same with the second pipette by the liquid suction in No. three centrifuge tubes simultaneously
It is transferred in No. four DNA adsorption columns, is then centrifuged for, at this moment Plasmid DNA can be adsorbed onto DNA adsorption column on DNA combination film, and be filtered
The waste collection crossed is in the lower part of DNA adsorption column.Then rotation liquid relief disk makes the 4th liquid-feeding tube successively be moved to No. two DNA absorption
The first washing lotion containing isopropanol is simultaneously added into DNA adsorption column by controlling solenoid valve for the top of column and No. four DNA adsorption columns
A removal foreign protein is cleaned, is then centrifuged for, is rotated further by liquid relief disk and is added from the 5th liquid-feeding tube into DNA adsorption column containing second
Second washing lotion of alcohol, washes twice or more and removes foreign protein, be then centrifuged for, artificial to take out DNA adsorption column top, and collection is had waste liquid
DNA adsorption column lower part take out abandon, change and put a new DNA collecting pipe, the DNA adsorption column top containing Plasmid DNA is put
Enter in DNA collecting pipe, is separately added into eluent into No. two and No. four DNA collecting pipes from the 6th liquid-feeding tube, is then centrifuged for eluting
Afterwards, pure Plasmid DNA is obtained in DNA collecting pipe, artificial collecting pipe of the taking-up containing Plasmid DNA saves backup.It is primary in this way
Operation can extract the Plasmid DNA of two volumes, increase yield, saved the time.It is above-mentioned by taking four centrifuge tubes as an example, but root
According to actual needs, more centrifugation tube seats can be set in centrifugal seat also can according to need, together with increasing higher yield
When extract several different Plasmid DNA.
Beneficial effects of the present invention: structure of the invention is compact, and the entire process for extracting plasmid is mainly by machine come automatic
It completes, does not need manually to participate in, in plasmid extraction process, each solution and washing lotion can be adjusted by modifying the parameter of controller
Additional amount, centrifugation time and oscillation intensity can also be adjusted, the wash number of adjustable cleaning solution, to improve the matter extracted
The quality of grain.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the structural schematic diagram of the Centrifugal vibration device in invention.
Appended drawing reference in above-mentioned attached drawing is as follows: 1 is master controller, and 2 be centrifugal seat, and 3 be centrifugation tube seat, and 4 be location sense
Device is answered, 5 be sensor, and 6 is go up and down gland, and 7 be centrifugation pipe close, and 8 be L bracket, and 9 be liquid relief disk, and 10 be liquid-feeding tube, and 11 be suction
Liquid pipe, 12 be solenoid valve, and 13 be waste liquid tank, and 14 be pedestal, and 15 be vibrating motor, and 16 be vibrating seat, and 17 be centrifuge.
Specific embodiment
With reference to the accompanying drawing 1, a specific embodiment of the invention is further elaborated in attached drawing 2:
(embodiment 1)
The labor-saving automatic plasmid extraction element of one kind as shown in Figure 1 comprising master controller 1, Centrifugal vibration dress
Set and be respectively arranged at the liquid adding and extracting device and lifting gland rotary lifting mechanism of Centrifugal vibration device two sides, centrifugation vibration
It is provided with centrifugal seat 2 on dynamic device, multiple centrifugation tube seats 3 are provided in centrifugal seat 2, setting is fixed by one of centrifugation tube seat 3
Position inductor 4, is provided with sensor 5 corresponding with positioning inductor 4, the lifting gland rotary lifter in centrifugal seat 2
Structure includes rotatable bracket and liftable lifting gland 6, and bracket is L shape, and lifting gland 6 is connected on bracket, buck
Centrifugation pipe close 7 corresponding with centrifugation tube seat 3 is provided below in lid 6, and liquid adding and extracting device includes rotatable L bracket 8 and sets
The liquid relief disk 9 being placed in L bracket 8 is provided with multiple liquid-feeding tubes 10 and pipette 11 on liquid relief disk 9, connects on liquid-feeding tube 10
Solenoid valve 12.
As shown in Fig. 2, in aforementioned schemes, Centrifugal vibration device include pedestal 14, vibrating motor 15, vibrating seat 16 and from
Scheming 17.Vibrating seat 16 is driven to vibrate by vibrating motor 15, centrifuge 17 is arranged on vibrating seat 16, can by this set
To realize the operation of vibration and centrifugation, simplifies the structure of automatic plasmid extraction element, keep whole device structure more compact.
In aforementioned schemes, it is provided with 6 liquid-feeding tubes 10 and two pipette 11 on liquid adding and extracting device, is total in centrifugal seat 2
4 centrifugation tube seats 3 are set, goes up and down and is correspondingly arranged 4 centrifugation pipe closes 7 below gland 6.
In scheme above-mentioned, it is located at liquid adding and extracting for the ease of focusing on to waste liquid, in the centrifugal seat 2 and fills
It sets and is nearby provided with waste liquid tank 13.
The working principle of above-mentioned labor-saving automatic plasmid extraction element are as follows: Centrifugal vibration is controlled by main controller 1
The rotation and vibration of device, the rotation of liquid adding and extracting device and lifting and in centrifuge tube inject and take out liquid, buck
The rotation and lifting of lid rotary lifting mechanism, the switch of solenoid valve 12, what the compression of centrifugation pipe close 7 and release and centrifugation were covered opens
It closes.
It may be provided with even number centrifugation tube seat 3 in centrifugal seat 2 as needed, in use, centrifuge tube and DNA are adsorbed intercolumniation
Every being put into centrifugation tube seat 3, it is illustrated for four centrifugation tube seats 3 are set in centrifugal seat 2 below.
It is No.1 to No. four that four centrifugation tube seats, which are sequentially numbered,；There is positioning inductor 4 by No.1 centrifugation tube seat 3, with centrifugation
Corresponding sensor 5 can be parked in automatically on the position of No.1 after incuding on workbench.When extracting plasmid, firstly, manually will be clean
Net No.1 centrifuge tube and No. three centrifuge tubes are respectively put into No.1 centrifugation tube seat 3 and No. three centrifugation tube seats 3, and No. two DNA are inhaled
Attached column and No. four DNA adsorption columns are respectively put into 3 in No. two centrifugation tube seats 3 and four No. three centrifugation tube seats, and will ferment
The inoculum (about 1.5mL) of equal volume is added in No.1 and No. three centrifuge tubes, then rotation lifting gland rotation and lifting
Lifting gland 6, is turned to the top of centrifugal seat 2 by the bracket of mechanism from the side horizontal of centrifugal seat 2, then moves down lifting gland 6,
It is pressed on four corresponding centrifuge tubes after four centrifuge tubes 7 plug being placed in below lifting gland 6 in advance is unclamped, then props up
Frame moves up and horizontally rotates lifting gland 6 to original position.Then, fasten centrifugation lid, Centrifugal vibration device to No.1 and
Inoculum rotating centrifugal in No. three centrifuge tubes.
After the completion of centrifugation, centrifugation lid is opened, and is gone up and down gland 6 from the side horizontal of centrifugal seat 2 and is turned to the upper of centrifugal seat 2
Face, then move down four centrifugation pipe closes 7 while unclamping and being fixed on lifting gland 6 in the following, lifting gland 6 moves up, then level turns
Bracket is moved to original position, at this moment bacterial precipitation is in the bottom of No.1 and No. three centrifuge tubes.
The L bracket 8 of subsequent liquid adding and extracting device rotates, and liquid relief disk 9 is horizontally rotated the upper surface of centrifugal seat 2, then turn
Dynamic liquid relief disk 9, turns to the first waste liquid suction pipe right above No.1 centrifuge tube.Liquid relief disk 9 is moved down, by 11 end of the first waste liquid suction pipe
It holds the long plastic tube disposably loaded onto advance slowly to enter No.1 centrifuge tube, supernatant fluid therein is slowly blotted, so
Waste liquid is discharged in waste liquid tank 13 to 13 top of waste liquid tank, is rotated further by liquid relief disk 9, make the first waste liquid suction pipe by rotation liquid relief disk 9 afterwards
11 turn to right above No. three centrifuge tubes.It moves down liquid relief disk and the long plastic tube of 11 lower end of the first waste liquid suction pipe is slowly entered No. three
Supernatant fluid therein is also slowly blotted and is discharged in waste liquid tank 13 by centrifuge tube.
Then liquid relief disk 9 rotates, and the first liquid-feeding tube 10 is made to be moved to right above No.1 centrifuge tube and control by solenoid valve 12
Quantitative 250 μ l of re-suspension liquid is added in system.Then plus the re-suspension liquid of equal volume is into No. three centrifuge tubes, in the way described above, will
Centrifugation pipe close 7 is pressed on centrifuge tube.Start Centrifugal vibration device, centrifuge tube acutely vibrates 1 minute, hangs microorganism uniformly
It is floating.
Rotating liquid relief disk 9 again is moved respectively to the second liquid-feeding tube above No.1 centrifuge tube and No. three centrifuge tubes and passes through electricity
The 250 μ l of bacteria lysis solution newly prepared is added in the control of magnet valve 12, is gently shaken by Centrifugal vibration device according to above-mentioned method
Swinging centrifuge tube mixes solution, and finally rotating liquid relief disk 9 makes third liquid-feeding tube be moved respectively to No.1 centrifuge tube and No. three centrifugations
It is added above pipe and by the control of solenoid valve 12 ice-cold for neutralizing the 350 μ l of neutralization solution of pH.Pass through Centrifugal vibration
Device according to above-mentioned method gently vibrate centrifuge tube make solution mix after be centrifuged.
After centrifugation, mobile and rotation liquid relief disk 9, the length disposably for loading onto 11 end of the second pipette in advance
Plastic tube extends in No.1 centrifuge tube and the supernatant fluid wherein containing Plasmid DNA is slowly sucked out, and is rotated further by liquid relief disk 9 for second
The supernatant fluid containing Plasmid DNA in pipette 11 is slowly added in No. two DNA adsorption columns.It is same with the second pipette by three
Liquid in number centrifuge tube is sucked out and is transferred in No. four DNA adsorption columns, is then centrifuged for, and at this moment Plasmid DNA can be adsorbed onto DNA suction
In attached column on DNA combination film, and the waste collection filtered is in the lower part of DNA adsorption column.
Then liquid relief disk 9 is rotated, the 4th liquid-feeding tube is made successively to be moved to No. two DNA adsorption columns and No. four DNA adsorption columns
Top simultaneously the first washing lotion containing isopropanol is added into DNA adsorption column is cleaned by controlling solenoid valve 12 and remove the egg that cleans for one time
It is white, it is then centrifuged for.
It is rotated further by liquid relief disk 9 and adds the second washing lotion containing ethyl alcohol into DNA adsorption column from the 5th liquid-feeding tube, wash twice
The above removal foreign protein, is then centrifuged for, artificial to take out DNA adsorption column top, will collect the DNA adsorption column lower part for having waste liquid and takes out
It abandons, changes and put a new DNA collecting pipe, the DNA adsorption column top containing Plasmid DNA is put into DNA collecting pipe.
Eluent is separately added into No. two and No. four DNA collecting pipes from the 6th liquid-feeding tube, after being then centrifuged for elution,
Pure Plasmid DNA is obtained in DNA collecting pipe, artificial collecting pipe of the taking-up containing Plasmid DNA saves backup.
Once-through operation can extract the Plasmid DNA of two volumes in this way, increase yield, save the time.It is above-mentioned with four
For a centrifuge tube, but according to actual needs, 2 can be set more centrifugation tube seats 3 in centrifugal seat, to increase higher production
Amount, also can according to need, while extracting several different Plasmid DNA.
Claims (1)
1. a kind of labor-saving automatic plasmid extraction element, it is characterised in that: it includes master controller (1), Centrifugal vibration dress
Set and be respectively arranged at the liquid adding and extracting device and lifting gland rotary lifting mechanism of Centrifugal vibration device two sides, centrifugation vibration
Centrifugal seat (2) are provided on dynamic device；Multiple centrifugation tube seats (3) are provided in centrifugal seat (2), one of centrifugation tube seat (3)
Side setting positioning inductor (4)；Sensor (5) corresponding with positioning inductor (4) is provided in centrifugal seat；The buck
Lid rotary lifting mechanism includes rotatable bracket and liftable lifting gland (6)；Bracket is L shape；Go up and down gland (6) connection
On bracket；Centrifugation pipe close (7) corresponding with centrifugation tube seat (3) is provided with below lifting gland (6)；Liquid adding and extracting device packet
The liquid relief disk (9) for including rotatable L bracket (8) and being set on L bracket (8)；Liquid relief disk is provided with multiple liquid feedings on (9)
Manage (10) and pipette (11)；Solenoid valve (12) are connected on liquid-feeding tube (10)；
The Centrifugal vibration device includes pedestal (14), vibrating motor (15), vibrating seat (16) and centrifuge (17)；
The centrifugation tube seat (3) being arranged in the centrifugal seat (2) is even number；Quantity is correspondingly arranged below lifting gland (6)
The identical centrifugation pipe close (7) with centrifugation tube seat (3)；
It is located at liquid adding and extracting device in the centrifugal seat (2) and is nearby provided with waste liquid tank (13)；
The master controller (1) is used to control rotation and vibration, the rotation of liquid adding and extracting device and lifting of Centrifugal vibration device
And rotation and lifting to being injected in centrifuge tube and taking out liquid, go up and down gland rotary lifting mechanism, solenoid valve (12) are opened
It closes, the switch that the compression and release and centrifugation for being centrifuged pipe close (7) are covered；
Pipette (11) includes the first waste liquid suction pipe and the second pipette, and disposable long plastics are loaded onto end in advance
Pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510339040.3A CN104893967B (en) | 2013-11-29 | 2013-11-29 | Labor-saving automatic plasmid extraction element |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510339040.3A CN104893967B (en) | 2013-11-29 | 2013-11-29 | Labor-saving automatic plasmid extraction element |
| CN201310637644.7A CN103602582B (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid-extracting device |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310637644.7A Division CN103602582B (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid-extracting device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104893967A CN104893967A (en) | 2015-09-09 |
| CN104893967B true CN104893967B (en) | 2019-07-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510338858.3A Pending CN104893966A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extraction device with compact structure |
| CN201510339212.7A Pending CN104893968A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extraction device with high extraction quality |
| CN201510339040.3A Active CN104893967B (en) | 2013-11-29 | 2013-11-29 | Labor-saving automatic plasmid extraction element |
| CN201510339213.1A Pending CN104928166A (en) | 2013-11-29 | 2013-11-29 | Automatic parameter-adjustable plasmid extracting device |
| CN201510339214.6A Pending CN104893969A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extracting device with compact structure |
| CN201310637644.7A Expired - Fee Related CN103602582B (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid-extracting device |
| CN201510338181.3A Pending CN105018340A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extracting apparatus with high extracting quality |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510338858.3A Pending CN104893966A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extraction device with compact structure |
| CN201510339212.7A Pending CN104893968A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extraction device with high extraction quality |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510339213.1A Pending CN104928166A (en) | 2013-11-29 | 2013-11-29 | Automatic parameter-adjustable plasmid extracting device |
| CN201510339214.6A Pending CN104893969A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extracting device with compact structure |
| CN201310637644.7A Expired - Fee Related CN103602582B (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid-extracting device |
| CN201510338181.3A Pending CN105018340A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extracting apparatus with high extracting quality |
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| Publication number | Priority date | Publication date | Assignee | Title |
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- 2013-11-29 CN CN201510339212.7A patent/CN104893968A/en active Pending
- 2013-11-29 CN CN201510339040.3A patent/CN104893967B/en active Active
- 2013-11-29 CN CN201510339213.1A patent/CN104928166A/en active Pending
- 2013-11-29 CN CN201510339214.6A patent/CN104893969A/en active Pending
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| CN1582191A (en) * | 2001-12-28 | 2005-02-16 | 株式会社日立高新技术 | Extractor, chemical analyzer and chemical analyzing method |
| CN2593911Y (en) * | 2002-12-13 | 2003-12-24 | 黄晓军 | Vertical scraper discharging centrifuge with liftable rotary cover |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104893968A (en) | 2015-09-09 |
| CN104893969A (en) | 2015-09-09 |
| CN103602582A (en) | 2014-02-26 |
| CN104893967A (en) | 2015-09-09 |
| CN105018340A (en) | 2015-11-04 |
| CN103602582B (en) | 2015-07-15 |
| CN104928166A (en) | 2015-09-23 |
| CN104893966A (en) | 2015-09-09 |
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