CN208898865U - A kind of nucleic acid-extracting apparatus - Google Patents
A kind of nucleic acid-extracting apparatus Download PDFInfo
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- CN208898865U CN208898865U CN201821319535.5U CN201821319535U CN208898865U CN 208898865 U CN208898865 U CN 208898865U CN 201821319535 U CN201821319535 U CN 201821319535U CN 208898865 U CN208898865 U CN 208898865U
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- bar magnet
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- 230000007246 mechanism Effects 0.000 claims abstract description 65
- 238000000605 extraction Methods 0.000 claims abstract description 59
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 50
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 27
- 230000000149 penetrating effect Effects 0.000 claims abstract description 22
- 230000001360 synchronised effect Effects 0.000 claims description 34
- 230000003373 anti-fouling effect Effects 0.000 claims description 28
- 239000000284 extract Substances 0.000 claims description 21
- 238000004659 sterilization and disinfection Methods 0.000 claims description 19
- 238000002347 injection Methods 0.000 claims description 11
- 239000007924 injection Substances 0.000 claims description 11
- 230000005611 electricity Effects 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 230000000844 anti-bacterial effect Effects 0.000 claims description 2
- 238000012546 transfer Methods 0.000 abstract description 6
- 238000011534 incubation Methods 0.000 abstract description 5
- 150000007523 nucleic acids Chemical class 0.000 description 13
- 102000039446 nucleic acids Human genes 0.000 description 13
- 108020004707 nucleic acids Proteins 0.000 description 13
- 238000000034 method Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 230000002070 germicidal effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 3
- 229940033663 thimerosal Drugs 0.000 description 3
- 239000011324 bead Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- -1 antifouling cover Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of nucleic acid-extracting apparatus, frame mid portion is connected with penetrating orbit, crossbeam arm is connected on penetrating orbit, the side of crossbeam arm is connected with clamping device, the other side of crossbeam arm is connected with sample charging mechanism, and clamping device carries out Y-direction along crossbeam arm and moves, and it is mobile that clamping device itself carries out Z-direction, sample charging mechanism carries out Y-direction along crossbeam arm and moves, and it is mobile that sample charging mechanism itself carries out Z-direction；Needle carrier is equipped on front side of the upper surface of pedestal, needle carrier side is equipped with and washes punch block, punch block side is washed equipped with reagent carrier, reagent carrier side is equipped with sample carrier, sample carrier side is equipped with constant temperature carrier, constant temperature carrier side is equipped with deep-well plates carrier, and deep-well plates carrier side is equipped with PCR incubator, is connected with several extraction mechanisms on rear side of the upper surface of pedestal.The utility model realizes continuous automatic sample, reagent adding, transfer, incubation and extraction movement, moreover it is possible to realize and sterilize to the suction nozzle after using, extraction efficiency is high.
Description
Technical field
The utility model relates to a kind of nucleic acid-extracting apparatus, are related to nucleic acid extraction technical field.
Background technique
From after the DNA double spin model birth of Watson-Crick, biology has entered a completely new epoch,
Biological educational circles, either the progress detection of pathogenic microorganism, species identification, or carry out the Origin of Species, diversity evaluation and its
The research of affiliation, phyletic evolution etc. requires to isolate and purify nucleic acid first, therefore makes a living to being extracted into for DNA
All subjects carry out the basis of scientific research in the fields such as object field of medicaments even farming, forestry, husbandary and fishing.And high quality can be extracted
Nucleic acid is the key that then in nucleic acid molecules biological test, and the sensitivity of extracting method, specificity will also be directly related to subsequent
The success or failure of test.
Bar magnet method realizes isolating and purifying for nucleic acid by the transfer of magnetic bead, the general movement by bar magnet in instrument,
Come realize magnetic bead from sample lysate/combine liquid to cleaning solution, then arrive eluent transfer, thus be automatically performed nucleic acid separation with
The overall process of purifying.Existing nucleic acid extraction technical solution, can not achieve automatic continuous sample-adding, reagent adding, incubation and extraction
Etc. processes, cannot achieve the disinfection to suction nozzle, it usually needs carry out nucleic acid extraction between multiple isolated equipment, it is whole to extract
Low efficiency cannot reach sample-adding and extract full-automation.
Utility model content
The utility model is in view of the deficienciess of the prior art, provide a kind of nucleic acid-extracting apparatus, based on existing automatic
Change control technology, can be realized continuous automatic sample, reagent adding, transfer, incubation and extraction movement, while being also able to achieve to using
Suction nozzle disinfection afterwards, extraction efficiency are high.
The technical solution that the utility model solves above-mentioned technical problem is as follows: a kind of nucleic acid-extracting apparatus, the extraction dress
It sets including frame, the frame bottom end is connected with pedestal, and pedestal is equipped with barcode scanning mechanism, and the frame mid portion is connected with through rail
Road is connected with crossbeam arm on the penetrating orbit, and the side of the crossbeam arm is connected with clamping device, the other side of crossbeam arm
It is connected with sample charging mechanism, the clamping device carries out Y-direction along the crossbeam arm and moves, and clamping device itself carries out Z-direction movement, institute
It states sample charging mechanism to move along crossbeam arm progress Y-direction, it is mobile that sample charging mechanism itself carries out Z-direction；Before the upper surface of the pedestal
Side is equipped with needle carrier, and needle carrier side is equipped with and washes punch block, washes punch block side equipped with reagent carrier, reagent carrier side is equipped with sample
Carrier, sample carrier side are equipped with constant temperature carrier, and constant temperature carrier side is equipped with deep-well plates carrier, and deep-well plates carrier side is equipped with
The upper surface rear side of PCR incubator, pedestal is connected with several extraction mechanisms；The crossbeam arm drives institute by the penetrating orbit
Clamping device and sample charging mechanism X are stated to being displaced into the needle carrier, wash punch block, reagent carrier, sample carrier, constant temperature carrier, depth
The top of orifice plate carrier and PCR incubator.
As the preferred embodiment of nucleic acid-extracting apparatus, the frame mid portion is connected with fixed cross beam, the fixed cross beam and
The penetrating orbit is in hollow distribution on said frame, is connected with ultraviolet germicidal on the inside of fixed cross beam.Fixed cross beam with
Common group of penetrating orbit connects the structural strength that frame improves frame, while the ultraviolet germicidal on the inside of fixed cross beam can be nucleic acid
Extraction element carries out sterilizing.
As the preferred embodiment of nucleic acid-extracting apparatus, crossbeam arm upper end is connected with the first Y-direction sliding rail, and sample charging mechanism is logical
It crosses the first Y-direction sliding rail to connect with the crossbeam arm, sample charging mechanism is connected with sample-adding Y-direction driving motor by synchronous belt, is loaded Y-direction
Driving motor drives first Y-direction sliding rail of the sample charging mechanism along crossbeam arm upper end to move by synchronous belt；Crossbeam arm upper end connects
It is connected to the second Y-direction sliding rail, the second Y-direction sliding rail and the first Y-direction sliding rail are arranged in parallel, and clamping device passes through the second Y-direction sliding rail and institute
The connection of crossbeam arm is stated, clamping device is connected with clamping Y-direction driving motor by synchronous belt.Clamping Y-direction driving motor passes through synchronization
The second Y-direction sliding rail with driving clamping device along crossbeam arm upper end moves.
As the preferred embodiment of nucleic acid-extracting apparatus, the sample charging mechanism includes sample-adding Z-direction driving motor, the sample-adding Z
It is connected with sample-adding Z-direction driving screw rod to driving motor, sample-adding Z-direction driving screw rod is connected with injection linkage seat, injection linkage seat warp
Sample-adding cylinder body is connected with sample-adding pump, and the sample-adding pump is connected with sample needle.It is loaded Z-direction driving motor and spiral shell is driven by sample-adding Z-direction
Bar driving injection linkage seat moves up and down, and injection linkage seat drive sample-adding cylinder body realizes the sample-adding of sample needle by sample-adding pump.
As the preferred embodiment of nucleic acid-extracting apparatus, the clamping device includes clamping Z-direction motor, the clamping Z-direction electricity
Machine is connected with clamping Z-direction screw rod, is arranged with pinboard on the clamping Z-direction screw rod, the pinboard lower end is connected with mobile bottom
Plate, the movable base plate lower section are connected with crawl fixing seat, and the crawl fixing seat upper end is connected with crawl motor, the crawl
Motor is connected with T-type screw rod by synchronous belt, and finger attachment base, the finger attachment base connection are arranged on the T-type screw rod
There is holding finger.It clamps Z-direction motor and pinboard is driven by clamping Z-direction screw rod, pinboard drives movable base plate to move up and down, grabs
Take motor that the finger attachment base on T-type screw rod is driven to move by synchronous belt, to realize that the opening and closing of holding finger are dynamic
The completion of work.
As the preferred embodiment of nucleic acid-extracting apparatus, the extraction mechanism include extract pedestal, antifouling cover, extract carrier,
Motor fixing plate, bar magnet fixed pin, fixed punch block, bar magnet set and bar magnet stock, extract two sides of base and are formed with the first slider bar
Hole, antifouling cover pass through first slider bar hole and move above the extraction pedestal；Mentioning on the inside of first slider bar hole
It takes and is formed with the second slider bar hole on pedestal, extract carrier and pass through the movement of second slider bar hole above the extraction pedestal
And it is in the lower section of the antifouling cover, it extracts and is connected with heating component and vibration component below carrier；It extracts and passes through on rear side of pedestal
Sliding rail connects the motor fixing plate；The motor fixing plate upper end, which passes through, fixes punch block and bar magnet set described in synchronous band connection
Frame, motor fixing plate lower end is connected with by synchronous belt extracts X to motor, and the bar magnet fixed pin is connected to the fixed punch block
Lower end, the bar magnet set are connected to bar magnet stock lower end.
As the preferred embodiment of nucleic acid-extracting apparatus, the antifouling cover is connected with antifouling driving motor, institute by synchronous belt
Antifouling driving motor is stated to be connected to below the extraction pedestal；The extraction carrier is connected with carrier motor, institute by synchronous belt
Carrier motor is stated to be connected to below the extraction pedestal；The fixed punch block is connected with punch block Z-direction motor by synchronous belt；It is described
Bar magnet stock is connected with bar magnet by synchronous belt and covers Z-direction motor.Antifouling cover, can be along extraction under the driving of antifouling driving motor
The first slider bar hole movement on pedestal, while extracting carrier can be along second on extraction pedestal under the drive of carrier motor
The movement of slider bar hole extracts carrier in and out of the lower section of antifouling cover to realize, the sample for preventing from extracting on carrier is contaminated.
As the preferred embodiment of nucleic acid-extracting apparatus, the bar magnet fixed pin uses single-row or biserial, and the bar magnet set is adopted
With single-row or biserial, the fixed punch block is located at the top of the bar magnet stock, and fixed punch block drives the bar magnet fixed pin to go out
Enter in the bar magnet set.
As the preferred embodiment of nucleic acid-extracting apparatus, the punch block of washing includes run-bake-needle fault baffle, the run-bake-needle fault baffle interior shape
At there is disinfection trough, the disinfection trough upper end is connected with run-bake-needle fault cover board, is formed with run-bake-needle fault slot on the run-bake-needle fault cover board.Disinfection trough can be with
Thimerosal is held, used suction nozzle is sterilized by disinfection trough, while being taken off suction nozzle by run-bake-needle fault slot.
The utility model relates to a kind of method for extracting nucleic acid the following steps are included:
Step 1: sample to be extracted is put into sample carrier, by scanning machine during sample carrier push-in pedestal
Structure is scanned record；
Step 2: clamping device grabs the deep-well plates being placed in deep-well plates carrier in the extraction carrier of extraction mechanism, so
Sample charging mechanism is moved to loading suction nozzle at needle carrier afterwards, is placed on the sample addition in sample carrier by suction nozzle and extracts in carrier
The corresponding hole of deep-well plates in；
Step 3: by suction nozzle the deep-well plates phase for being placed on and extracting in carrier is added in the reagent in reagent carrier by sample charging mechanism
In the hole answered, extraction mechanism carries out heating vibration, while being extracted by bar magnet fixed pin and bar magnet set；
Step 4: after the completion of extraction, sample charging mechanism is added the DNA completed is extracted in another deep-well plates, and then clamps
The deep-well plates for having DNA are put into PCR incubator by mechanism, and control is incubated for 85 DEG C of the temperature inside the box, after being incubated for 30min, by clamping machine
Deep-well plates are placed again into and extract in carrier by structure, purify DNA again；
Step 5: sample charging mechanism, which is moved to, washes punch block, through washing needle carrier disinfection trough sterilize after again by run-bake-needle fault slot into
Row run-bake-needle fault.
The utility model frame mid portion is connected with penetrating orbit, and crossbeam arm, the side of crossbeam arm are connected on penetrating orbit
It is connected with clamping device, the other side of crossbeam arm is connected with sample charging mechanism, and clamping device carries out Y-direction along crossbeam arm and moves, presss from both sides
It holds mechanism itself and carries out Z-direction movement, sample charging mechanism carries out Y-direction along crossbeam arm and moves, and it is mobile that sample charging mechanism itself carries out Z-direction；Base
Needle carrier is equipped on front side of the upper surface of seat, needle carrier side is equipped with and washes punch block, washes punch block side equipped with reagent carrier, reagent carrier
Side is equipped with sample carrier, and sample carrier side is equipped with constant temperature carrier, and constant temperature carrier side is equipped with deep-well plates carrier, and deep hole is onboard
Frame side is equipped with PCR incubator, is connected with several extraction mechanisms on rear side of the upper surface of pedestal；Crossbeam arm is driven by penetrating orbit
Needle carrier, to wash punch block, reagent carrier, sample carrier, constant temperature carrier, deep hole onboard to being displaced by clamping device and sample charging mechanism X
The top of frame and PCR incubator.The utility model be based on existing automated control technology, can be realized continuous automatic sample,
Reagent adding, transfer, incubation and extraction movement, while being also able to achieve to the suction nozzle disinfection after using, extraction efficiency is high, and precision is high.
Detailed description of the invention
Fig. 1 is embodiment amplifying nucleic acid extraction element structural schematic diagram；
Fig. 2 is the nucleic acid-extracting apparatus structural schematic diagram that emphasis illustrates internal structure in embodiment；
Fig. 3 is the structural schematic diagram of pedestal upper surface in embodiment；
Fig. 4 is embodiment middle cross beam arm and sample charging mechanism schematic diagram；
Fig. 5 is embodiment middle cross beam arm and clamping device schematic diagram；
Fig. 6 is extraction mechanism schematic diagram in embodiment；
Fig. 7 is method for extracting nucleic acid flow chart；
Wherein, 1, frame；2, pedestal；3, barcode scanning mechanism；4, penetrating orbit；5, crossbeam arm；6, clamping device；7, it is loaded machine
Structure；8, needle carrier；9, punch block is washed；10, reagent carrier；11, sample carrier；12, constant temperature carrier；13, deep-well plates carrier；14,PCR
Incubator；15, extraction mechanism；16, fixed cross beam；17, ultraviolet germicidal；18, the first Y-direction sliding rail；19, sample-adding Y-direction driving electricity
Machine；20, the second Y-direction sliding rail；21, Y-direction driving motor is clamped；22, it is loaded Z-direction driving motor；23, sample-adding Z-direction drives screw rod；
24, linkage seat is injected；25, it is loaded cylinder body；26, sample-adding pump；27, sample needle；28, Z-direction motor is clamped；29, Z-direction screw rod is clamped；
30, pinboard；31, movable base plate；32, fixing seat is grabbed；33, motor is grabbed；34, T-type screw rod；35, finger attachment base；36,
Holding finger；37, pedestal is extracted；38, antifouling cover；39, carrier is extracted；40, motor fixing plate；41, bar magnet fixed pin；42, solid
Determine punch block；43, bar magnet set；44, bar magnet stock；45, the first slider bar hole；46, the second slider bar hole；47, X is extracted to motor；
48, antifouling driving motor；49, carrier motor；50, punch block Z-direction motor；51, bar magnet covers Z-direction motor；52, run-bake-needle fault baffle；53, disappear
Poison tank；54, run-bake-needle fault cover board；55, run-bake-needle fault slot.
Specific embodiment
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, with reference to the accompanying drawing to this
The specific embodiment of utility model is described in detail.Many details are explained in the following description in order to abundant
Understand the utility model.But the utility model can be implemented with being much different from other way described herein, this field
Technical staff can do similar improvement without prejudice to the utility model connotation, therefore the utility model is not by following public affairs
The limitation for the specific embodiment opened.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" packet
Include any and all combinations of one or more related listed items.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5 and Fig. 6, a kind of nucleic acid-extracting apparatus, the extraction element includes frame
1,1 bottom end of frame is connected with pedestal 2, and pedestal 2 is equipped with barcode scanning mechanism 3, is connected with penetrating orbit 4 in the middle part of the frame 1,
It is connected with crossbeam arm 5 on the penetrating orbit 4, the side of the crossbeam arm 5 is connected with clamping device 6, and other the one of crossbeam arm 5
Side is connected with sample charging mechanism 7, and the clamping device 6 carries out Y-direction along the crossbeam arm 5 and moves, and clamping device 6 itself carries out Z-direction
Mobile, the sample charging mechanism 7 carries out Y-direction along the crossbeam arm 5 and moves, and it is mobile that sample charging mechanism 7 itself carries out Z-direction；The pedestal 2
Upper surface on front side of be equipped with needle carrier 8,8 side of needle carrier is equipped with and washes punch block 9, washes 9 side of punch block equipped with reagent carrier 10, reagent
10 side of carrier is equipped with sample carrier 11, and 11 side of sample carrier is equipped with constant temperature carrier 12, and 12 side of constant temperature carrier is equipped with deep hole
Onboard frame 13,13 side of deep-well plates carrier are equipped with PCR incubator 14, are connected with several extraction mechanisms on rear side of the upper surface of pedestal 2
15；The crossbeam arm 5 drives the clamping device 6 and sample charging mechanism 7 to realize that X is described to being displaced by the penetrating orbit 4
Needle carrier 8 washes punch block 9, reagent carrier 10, sample carrier 11, constant temperature carrier 12, deep-well plates carrier 13 and PCR incubator 14
Top.
In one embodiment of nucleic acid-extracting apparatus, fixed cross beam 16, the fixed cross beam are connected in the middle part of the frame 1
16 and the penetrating orbit 4 be distributed on the frame 1 in hollow, be connected with ultraviolet germicidal 17 on the inside of fixed cross beam 16.
Fixed cross beam 16 connects the structural strength that frame 1 improves frame 1, while the purple of 16 inside of fixed cross beam with 4 common groups of penetrating orbit
Outer bactericidal lamp 17 can carry out sterilizing for nucleic acid-extracting apparatus.
In one embodiment of nucleic acid-extracting apparatus, 5 upper end of crossbeam arm is connected with the first Y-direction sliding rail 18, is loaded machine
Structure 7 is connect by the first Y-direction sliding rail 18 with the crossbeam arm 5, and sample charging mechanism 7 is connected with sample-adding Y-direction driving electricity by synchronous belt
Machine 19, sample-adding Y-direction driving motor 19 drive first Y-direction sliding rail 18 of the sample charging mechanism 7 along 5 upper end of crossbeam arm to transport by synchronous belt
It is dynamic；5 upper end of crossbeam arm is connected with the second Y-direction sliding rail 20, and the second Y-direction sliding rail 20 and the first Y-direction sliding rail 18 are arranged in parallel, folder
It holds mechanism 6 to connect by the second Y-direction sliding rail 20 with the crossbeam arm 5, clamping device 6 is connected with clamping Y-direction by synchronous belt and drives
Dynamic motor 21.It clamps Y-direction driving motor 21 and drives clamping device 6 along the second Y-direction sliding rail 20 of 5 upper end of crossbeam arm by synchronous belt
Movement.
In one embodiment of nucleic acid-extracting apparatus, the sample charging mechanism 7 includes sample-adding Z-direction driving motor 22, described to add
Sample Z-direction driving motor 22 is connected with sample-adding Z-direction driving screw rod 23, and sample-adding Z-direction driving screw rod 23 is connected with injection linkage seat 24, note
It penetrates linkage seat 24 and is connected with sample-adding pump 26 through being loaded cylinder body 25, the sample-adding pump 26 is connected with sample needle 27.It is loaded Z-direction driving electricity
Machine 22 drives the driving injection linkage seat 24 of screw rod 23 to move up and down by sample-adding Z-direction, and injection linkage seat 24 drives sample-adding cylinder body 25
The sample-adding of sample needle 27 is realized by sample-adding pump 26.
In one embodiment of nucleic acid-extracting apparatus, the clamping device 6 includes clamping Z-direction motor 28, the clamping Z-direction
Motor 28 is connected with clamping Z-direction screw rod 29, is arranged with pinboard 30,30 lower end of pinboard on the clamping Z-direction screw rod 29
It is connected with movable base plate 31, crawl fixing seat 32 is connected with below the movable base plate 31,32 upper end of crawl fixing seat connects
It is connected to crawl motor 33, the crawl motor 33 is connected with T-type screw rod 34 by synchronous belt, is arranged on the T-type screw rod 34
Finger attachment base 35, the finger attachment base 35 are connected with holding finger 36.It clamps Z-direction motor 28 and passes through clamping Z-direction screw rod 29
Pinboard 30 is driven, pinboard 30 drives movable base plate 31 to move up and down, and crawl motor 33 drives T-type screw rod 34 by synchronous belt
On finger attachment base 35 move, thus realize holding finger 36 opening and closing movement completion.
In one embodiment of nucleic acid-extracting apparatus, the extraction mechanism 15 includes extracting pedestal 37, antifouling cover 38, extracting
Carrier 39, motor fixing plate 40, bar magnet fixed pin 41, fixed punch block 42, bar magnet set 43 and bar magnet stock 44, extract 37 liang of pedestal
Side is formed with the first slider bar hole 45, and antifouling cover 38 passes through first slider bar hole 45 and moves on the extraction pedestal 37
Side；It is formed with the second slider bar hole 46 on the extraction pedestal 37 of first slider bar hole, 45 inside, carrier 39 is extracted and passes through institute
The movement of the second slider bar hole 46 is stated above the extraction pedestal 37 and is in the lower section of the antifouling cover 38, is extracted under carrier 39
Side is connected with heating component and vibration component (prior art)；It extracts and the motor fixing plate is connected by sliding rail on rear side of pedestal 37
40；40 upper end of motor fixing plate, which passes through, fixes punch block 42 and bar magnet stock 44, motor fixing plate 40 described in synchronous band connection
Lower end is connected with by synchronous belt extracts X to motor 47, and the bar magnet fixed pin 41 is connected to 42 lower end of fixed punch block, institute
It states bar magnet set 43 and is connected to 44 lower end of bar magnet stock.
In one embodiment of nucleic acid-extracting apparatus, the antifouling cover 38 is connected with antifouling driving motor by synchronous belt
48, the antifouling driving motor 48 is connected to 37 lower section of extraction pedestal；The extraction carrier 39 is connected with by synchronous belt
Carrier motor 49, the carrier motor 49 are connected to 37 lower section of extraction pedestal；The fixed punch block 42 is connected by synchronous belt
It is connected to punch block Z-direction motor 50；The bar magnet stock 44 is connected with bar magnet by synchronous belt and covers Z-direction motor 51.Antifouling cover 38 is anti-
Under the driving of dirty driving motor 48, it can be moved along the first slider bar hole 45 extracted on pedestal 37, while extracting carrier 39 and existing
It can move along the second slider bar hole 46 extracted on pedestal 37 under the drive of carrier motor 49, go out to realize and extract carrier 39
The lower section for entering antifouling cover 38, the sample for preventing from extracting on carrier 39 are contaminated.
In one embodiment of nucleic acid-extracting apparatus, the bar magnet fixed pin 41 uses single-row or biserial, the bar magnet set
43 use single-row or biserial, and the fixed punch block 42 is located at the top of the bar magnet stock 44, and punch block Z-direction motor 50 can make solid
Determining punch block 42 drives the bar magnet fixed pin 41 to enter and leave in the bar magnet set 43.Bar magnet set Z-direction motor 51 is equally able to achieve described
Bar magnet set 43 is separated with bar magnet fixed pin 41.
In one embodiment of nucleic acid-extracting apparatus, the punch block 9 of washing includes run-bake-needle fault baffle 52, in the run-bake-needle fault baffle 52
Portion is formed with disinfection trough 53, and 53 upper end of disinfection trough is connected with run-bake-needle fault cover board 54, is formed with run-bake-needle fault on the run-bake-needle fault cover board 54
Slot 55.Disinfection trough 53 can hold thimerosal, be sterilized by disinfection trough 53 to used suction nozzle, while will by run-bake-needle fault slot 55
Suction nozzle is taken off.
Referring to Fig. 7, the utility model relates to a kind of method for extracting nucleic acid, the extracting method is filled using above-mentioned extraction
Set, the extracting method the following steps are included:
S1: sample to be extracted is put into sample carrier 11, by scanning machine during the push-in pedestal 2 of sample carrier 11
Structure 3 is scanned record；
S2: clamping device 6 grabs the deep-well plates being placed in deep-well plates carrier 13 in the extraction carrier 39 of extraction mechanism 15,
Then sample charging mechanism 7 is moved to loading suction nozzle at needle carrier 8, and the sample addition in sample carrier 11 is placed on extraction by suction nozzle
In the corresponding hole of deep-well plates in carrier 39；
S3: by suction nozzle the deep-well plates for being placed on and extracting in carrier 39 are added in the reagent in reagent carrier 10 by sample charging mechanism 7
In corresponding hole, extraction mechanism 15 carries out heating vibration, while being extracted by bar magnet fixed pin 41 and bar magnet set 43；
S4: after the completion of extraction, sample charging mechanism 7 is added the DNA completed is extracted in another deep-well plates, then clamping device
6 are put into the deep-well plates for having DNA in PCR incubator 14, and control is incubated for 85 DEG C of the temperature inside the box, after being incubated for 30min, by clamping device
6 are placed again into deep-well plates in extraction carrier 39, purify DNA again；
S5: sample charging mechanism 7, which is moved to, washes punch block 9, and the disinfection trough 53 by washing needle carrier 8 passes through run-bake-needle fault slot 55 after sterilizing again
Carry out run-bake-needle fault.
It is connected with penetrating orbit 4 in the middle part of the utility model frame 1, crossbeam arm 5 is connected on penetrating orbit 4, crossbeam arm 5
Side is connected with clamping device 6, and the other side of crossbeam arm 5 is connected with sample charging mechanism 7, and clamping device 6 carries out Y along crossbeam arm 5
To movement, clamping device 6 itself carries out that Z-direction is mobile, and sample charging mechanism 7 carries out Y-direction along crossbeam arm 5 and moves, sample charging mechanism 7 itself into
Row Z-direction is mobile；Needle carrier 8 is equipped on front side of the upper surface of pedestal 2,8 side of needle carrier is equipped with and washes punch block 9, washes 9 side of punch block and be equipped with
Reagent carrier 10,10 side of reagent carrier are equipped with sample carrier 11, and 11 side of sample carrier is equipped with constant temperature carrier 12, constant temperature carrier
12 sides are equipped with deep-well plates carrier 13, and 13 side of deep-well plates carrier is equipped with PCR incubator 14, connection on rear side of the upper surface of pedestal 2
There are several extraction mechanisms 15；Crossbeam arm 5 drives clamping device 6 and sample charging mechanism 7X to being displaced into needle carrier by penetrating orbit 4
8, the top of punch block 9, reagent carrier 10, sample carrier 11, constant temperature carrier 12, deep-well plates carrier 13 and PCR incubator 14 is washed.Its
In, sample-adding Y-direction driving motor drives first Y-direction sliding rail 18 of the sample charging mechanism 7 along 5 upper end of crossbeam arm to move by synchronous belt, presss from both sides
It holds Y-direction driving motor 21 and drives second Y-direction sliding rail 20 of the clamping device 6 along 5 upper end of crossbeam arm to move by synchronous belt.It is loaded Z
Drive screw rod 23 that injection linkage seat 24 is driven to move up and down by sample-adding Z-direction to driving motor 22, injection linkage seat 24, which drives, to be added
Sample cylinder body 25 realizes the sample-adding of sample needle 27 by sample-adding pump 26.Antifouling cover 38, can under the driving of antifouling driving motor 48
It is moved along the first slider bar hole 45 extracted on pedestal 37, while extracting carrier 39 can be along mentioning under the drive of carrier motor 49
It takes the second slider bar hole 46 on pedestal 37 to move, extracts carrier 39 in and out of the lower section of antifouling cover 38 to realize, prevent from mentioning
Take the sample on carrier 39 contaminated.The disinfection trough 53 of the utility model can hold thimerosal, be used by 53 Duis of disinfection trough
The suction nozzle disinfection crossed, while being taken off suction nozzle by run-bake-needle fault slot 55.The utility model is based on existing automated control technology, energy
It enough realizes continuous automatic sample, reagent adding, transfer, incubation and extraction movement, while being also able to achieve to the suction nozzle disinfection after using,
Extraction efficiency is high, and precision is high.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it cannot be understood as the limitations to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (9)
1. a kind of nucleic acid-extracting apparatus, the extraction element includes frame, and the frame bottom end is connected with pedestal, and pedestal is equipped with
Barcode scanning mechanism, it is characterised in that: the frame mid portion is connected with penetrating orbit, and crossbeam arm, institute are connected on the penetrating orbit
The side for stating crossbeam arm is connected with clamping device, and the other side of crossbeam arm is connected with sample charging mechanism, and the clamping device is along institute
It states crossbeam arm and carries out Y-direction movement, clamping device itself carries out Z-direction movement, and the sample charging mechanism carries out Y-direction shifting along the crossbeam arm
Dynamic, it is mobile that sample charging mechanism itself carries out Z-direction；Needle carrier is equipped on front side of the upper surface of the pedestal, needle carrier side is equipped with and washes needle
Frame washes punch block side equipped with reagent carrier, and reagent carrier side is equipped with sample carrier, and sample carrier side is equipped with constant temperature carrier,
Constant temperature carrier side is equipped with deep-well plates carrier, and deep-well plates carrier side is equipped with PCR incubator, is connected on rear side of the upper surface of pedestal
Several extraction mechanisms；The crossbeam arm drives the clamping device and sample charging mechanism X to being displaced by the penetrating orbit
The top stated needle carrier, wash punch block, reagent carrier, sample carrier, constant temperature carrier, deep-well plates carrier and PCR incubator.
2. a kind of nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the frame mid portion is connected with fixed cross
Beam, the fixed cross beam and the penetrating orbit are in hollow distribution on said frame, are connected on the inside of fixed cross beam ultraviolet
Bactericidal lamp.
3. a kind of nucleic acid-extracting apparatus according to claim 1, it is characterised in that: crossbeam arm upper end is connected with first
Y-direction sliding rail, sample charging mechanism are connect by the first Y-direction sliding rail with the crossbeam arm, and sample charging mechanism is connected with sample-adding Y by synchronous belt
To driving motor；Crossbeam arm upper end is connected with the second Y-direction sliding rail, and the second Y-direction sliding rail and the first Y-direction sliding rail are arranged in parallel,
Clamping device is connect by the second Y-direction sliding rail with the crossbeam arm, and clamping device is connected with clamping Y-direction driving electricity by synchronous belt
Machine.
4. a kind of nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the sample charging mechanism includes sample-adding Z-direction
Driving motor, the sample-adding Z-direction driving motor are connected with sample-adding Z-direction driving screw rod, and sample-adding Z-direction driving screw rod is connected with injection connection
Dynamic seat, injection linkage seat are connected with sample-adding pump through being loaded cylinder body, and the sample-adding pump is connected with sample needle.
5. a kind of nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the clamping device includes clamping Z-direction
Motor, the clamping Z-direction motor are connected with clamping Z-direction screw rod, are arranged with pinboard, the switching on the clamping Z-direction screw rod
Plate lower end is connected with movable base plate, and crawl fixing seat, the crawl fixing seat upper end connection are connected with below the movable base plate
There is crawl motor, the crawl motor is connected with T-type screw rod by synchronous belt, is arranged with finger attachment base on the T-type screw rod,
The finger attachment base is connected with holding finger.
6. a kind of nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the extraction mechanism includes extracting base
Seat, antifouling cover extract carrier, motor fixing plate, bar magnet fixed pin, fixed punch block, bar magnet set and bar magnet stock, extract pedestal two
Side is formed with the first slider bar hole, and antifouling cover passes through first slider bar hole and moves above the extraction pedestal；Described
It is formed with the second slider bar hole on extraction pedestal on the inside of one slider bar hole, extracts carrier and is moved across second slider bar hole
Above the extraction pedestal and the lower section in the antifouling cover, extraction carrier lower section are connected with heating component and vibration group
Part；It extracts and the motor fixing plate is connected by sliding rail on rear side of pedestal；The motor fixing plate upper end passes through synchronous band connection institute
Fixed punch block and bar magnet stock are stated, motor fixing plate lower end is connected with by synchronous belt extracts X to motor, the bar magnet fixed pin
It is connected to the fixed punch block lower end, the bar magnet set is connected to bar magnet stock lower end.
7. a kind of nucleic acid-extracting apparatus according to claim 6, it is characterised in that: the antifouling cover passes through synchronous band connection
There is antifouling driving motor, the antifouling driving motor is connected to below the extraction pedestal；The extraction carrier passes through synchronous belt
It is connected with carrier motor, the carrier motor is connected to below the extraction pedestal；The fixed punch block passes through synchronous band connection
There is punch block Z-direction motor；The bar magnet stock is connected with bar magnet by synchronous belt and covers Z-direction motor.
8. a kind of nucleic acid-extracting apparatus according to claim 6, it is characterised in that: the bar magnet fixed pin using single-row or
Biserial, the bar magnet set use single-row or biserial, and the fixed punch block is located at the top of the bar magnet stock, and fixed punch block drives
The bar magnet fixed pin enters and leaves in the bar magnet set.
9. a kind of nucleic acid-extracting apparatus according to claim 1, it is characterised in that: the punch block of washing includes run-bake-needle fault baffle,
The run-bake-needle fault baffle interior is formed with disinfection trough, and the disinfection trough upper end is connected with run-bake-needle fault cover board, is formed on the run-bake-needle fault cover board
There is run-bake-needle fault slot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821319535.5U CN208898865U (en) | 2018-08-15 | 2018-08-15 | A kind of nucleic acid-extracting apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821319535.5U CN208898865U (en) | 2018-08-15 | 2018-08-15 | A kind of nucleic acid-extracting apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208898865U true CN208898865U (en) | 2019-05-24 |
Family
ID=66569360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821319535.5U Withdrawn - After Issue CN208898865U (en) | 2018-08-15 | 2018-08-15 | A kind of nucleic acid-extracting apparatus |
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| Country | Link |
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| CN (1) | CN208898865U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949505A (en) * | 2018-08-15 | 2018-12-07 | 烟台艾德康生物科技有限公司 | A kind of nucleic acid-extracting apparatus and method |
-
2018
- 2018-08-15 CN CN201821319535.5U patent/CN208898865U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949505A (en) * | 2018-08-15 | 2018-12-07 | 烟台艾德康生物科技有限公司 | A kind of nucleic acid-extracting apparatus and method |
| CN108949505B (en) * | 2018-08-15 | 2024-04-02 | 烟台艾德康生物科技有限公司 | Nucleic acid extraction device and method |
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