CN109554290A - A kind of drawer type biological sample scribing line culture apparatus - Google Patents
A kind of drawer type biological sample scribing line culture apparatus Download PDFInfo
- Publication number
- CN109554290A CN109554290A CN201811623314.1A CN201811623314A CN109554290A CN 109554290 A CN109554290 A CN 109554290A CN 201811623314 A CN201811623314 A CN 201811623314A CN 109554290 A CN109554290 A CN 109554290A
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- China
- Prior art keywords
- inoculator
- drawer type
- culture
- biological sample
- sided
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Links
- 239000012472 biological sample Substances 0.000 title claims abstract description 30
- 239000000523 sample Substances 0.000 claims abstract description 51
- 238000011081 inoculation Methods 0.000 claims abstract description 22
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 239000000284 extract Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
Abstract
The present invention relates to a kind of drawer type biological sample scribing line culture apparatus.Two inoculation arms of the culture apparatus, including two-sided culture plate, drawer type sample pool and inoculator, inoculator sequentially pass through inoculator fixing seat and culture tank, extend into the drawer type sample pool of specimen holder;Two-sided culture plate two sides is equipped with the culture tank being recessed inwardly, drawer type sample pool is equipped in the specimen holder of two-sided culture plate lower end, it extracts drawer type sample pool out and biological sample is added, two inoculation arms of inoculator are extend into sample pool, dip biological sample, inoculator is operated, carries out streak inoculation on the culture medium held in culture tank, use and its conveniently;Culture plate one end is equipped with the inoculator fixing seat for installing inoculator, and the other end is equipped with the specimen holder for installing drawer type sample pool, and culture tank is arranged between inoculator fixing seat and specimen holder, simple and reasonable.
Description
Technical field
The present invention relates to microculture detection technique fields, cross and cultivate more particularly, to a kind of drawer type biological sample
Device.
Background technique
In hospital clinical and medical research test, the blood to patient, sputum, excrement, urine, body fluid etc. are generally required
Sample is separately cultured and smear.Detection method typically now is will to carry out the pretreatment of early period to sample by craft,
By in sample streak inoculation to culture dish after the completion of pretreatment, then culture dish is transferred in incubator by hand and is cultivated.This
There are very big drawbacks during sequence of operations: since operator is in this case easily with sample contact, being easy to make
At infection, constitute a serious threat to the health of operator.In addition, during manual operation, also due to above-mentioned sample
Bad smell can be issued, therefore it will affect analysis and the operation of laboratory technician, thus the accuracy of impact analysis result.It is Chinese special
Benefit number be 200520096773.0 " biological sample is separately cultured device " utility model patent, disclose a kind of pair of biological sample into
Row acquisition simultaneously carries out the culture apparatus that is separately cultured of bacterium to sample, the patent by two pieces of bottoms to the culture plate at bottom, sample pool, connect
Kind device composition.Filling solid medium on culture plate, the two sides of culture plate are equipped with inoculator, and the lower end of inoculator connects inoculation
Ring, oese extend to the sample pool below culture plate, and the upper end of inoculator connects driving handle.This structure is separately cultured
Instrument has the drawback that: the characteristics of due to this structure, sample pool is only used for storage sample, be conducive to oese pick sample into
Row streak inoculation, but to the biological sample in sample pool, also to carry out smear, dyeing, it is also necessary to pipettor in sample pool
Middle sampling, which is filled on glass slide, carries out smear, very inconvenient.And smear is carried out to the sample in sample pool to confirm that infection
Bacterium belongs to G+ or G-, is very important one during microorganism detection in order to send level-one report in time to clinical
Link.
Summary of the invention
In order to solve the above problem, the present invention provides that a kind of structure is simple, drawer type biological sample subregion easy to use is drawn
Line culture apparatus.
The technical solution adopted by the present invention is that: a kind of drawer type biological sample scribing line culture apparatus, including two-sided culture plate,
The two-sided culture plate two sides is equipped with the culture tank for holding culture medium, it is characterised in that: the two-sided culture plate lower end
Equipped with specimen holder, it is equipped with sliding slot in the specimen holder, the drawer type sample pool for holding biological sample is equipped in the sliding slot,
The drawer type sample pool is connected to the culture tank on two-sided culture plate two sides;The drawer type sample pool is from one end of specimen holder
Insertion, the drawer cover for separating the culture tank and drawer type sample pool are inserted into from the other end of specimen holder.
Preferably, the two-sided culture plate upper end is equipped with the inoculator fixing seat for installing inoculator, the culture
Slot be arranged between inoculator fixing seat and specimen holder, two inoculation arms of the inoculator sequentially pass through inoculator fixing seat with
Culture tank is extend into the drawer type sample pool in specimen holder.
Preferably, the two-sided culture plate two sides is equipped with the cover board for covering culture tank.
Preferably, being equipped with mutual corresponding convex ribs on the cover board inner sidewall and two-sided culture plate lateral wall, and logical
Convex ribs fixing card is crossed to be connected together.
Further, the cover board is equipped with triangle limit plate, and the inoculator fixing seat is equipped with and triangle limit plate
The limiting slot of cooperation.
Preferably, the two-sided culture plate is made up of the identical two halves of structure the formation being clamped.
Further, the two-sided culture plate upper and lower ends are equipped with the inoculation hole passed through for inoculation arm.
Further, the inoculator fixing seat be equipped with the card slot that is clamped with inoculator cross bar and with inoculator gag lever post
The location hole of cooperation.
Further, the inoculator is equipped with operation handle.
The beneficial effect that the present invention obtains is: two-sided culture plate two sides is equipped with the culture tank being recessed inwardly, two-sided culture
Drawer type sample pool is equipped in the specimen holder of plate lower end, biological sample is added in extraction drawer type sample pool, and two of inoculator connect
Kind arm is extend into sample pool, dips biological sample, operates inoculator, and scribing line is carried out on the culture medium held in culture tank and is connect
Kind, use and its conveniently;Culture plate one end is equipped with the inoculator fixing seat for installing inoculator, and the other end is equipped with for installing
The specimen holder of drawer type sample pool, culture tank is arranged between inoculator fixing seat and specimen holder, simple and reasonable;Two-sided training
It supports plate and is made up of the identical two halves of structure the formation being clamped, two-sided culture plate two sides is equipped with the lid for covering culture tank
Plate, cover board and culture plate are clamped by the way that convex ribs are fixed, are equipped with sliding slot, specimen holder inner sidewall on specimen holder fixed mechanism two sides
It is equipped with the cunning muscle cooperated with sliding slot, it is easy for assemble or unload.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention;
Fig. 2 is the state diagram of drawer type sample pool pull-out type;
Fig. 3-4 is the schematic diagram of two kinds of different conditions of the invention.
Specific embodiment
The present invention will be further explained in the following with reference to the drawings and specific embodiments.
As shown in Figs 1-4, a kind of drawer type biological sample scribing line culture apparatus, including two-sided culture plate 1, drawer type sample
Pond 2 and inoculator 3, two-sided culture plate 1 are placed vertically, and the upper end of two-sided culture plate 1 is equipped with the inoculator for installing inoculator 3
Fixing seat 11, lower end is equipped with the specimen holder 13 for installing drawer type sample pool 2, and (drawer type sample pool 2 can be in specimen holder 13
Interior propulsion or extraction), two-sided 1 two sides of culture plate is equipped with the culture tank 12 being recessed inwardly, and culture tank 12 is whole rounded, is used for
Culture medium is placed, culture tank 12 is arranged between inoculator fixing seat 11 and specimen holder fixed mechanism 12.Inoculator 3 is V shape knot
Structure, two inoculation arms 31 sequentially pass through inoculator fixing seat 11 and culture tank 12 from the inside of two-sided culture plate 1, extend into sample
In the drawer type sample pool 2 of seat 13, biological sample is added after extracting out in drawer type sample pool 2.
Two-sided 1 two sides of culture plate is equipped with the cover board 4 for covering culture tank 12,4 inner sidewall of cover board and two-sided culture plate 1
It is equipped with mutual corresponding convex ribs on lateral wall, by cover clamp 4, is connected to two-sided culture plate 1 and the fixation of cover board 4 by convex ribs
Together.In the present embodiment, the top of cover board 4 is equipped with triangle limit plate 41, and inoculator fixing seat 11 is equipped with and triangle limit plate
The limiting slot of 41 cooperations, the rounded portions 42 of cover board 4 and round culture tank 12 cooperate, and the rounded portions 42 of cover board 4 are equipped with outward
Limiting slot in the hand-held part 43 of protrusion, triangle limit plate 41 and inoculator fixing seat 11 cooperates, and accurate positioning is conducive to be inoculated with
Arm 31 can smoothly be extend into sample pool 21;By the way that hand-held part 43 is arranged, cover board 4 is facilitated to open.
Two-sided culture plate 1 is made up of the identical two halves of structure the formation being clamped, and two halves two-sided 1 bottom of culture plate is logical the bottom of to
The form fastening for crossing convex ribs and hole is connected together.
Sliding slot is equipped on 13 liang of medial surfaces of specimen holder, 2 lateral wall of drawer type sample pool is equipped with the cunning cooperated with sliding slot
Muscle.Drawer type sample pool 2 is under the cooperation of sliding slot and sliding muscle, and drawer type sample pool 2 can be extracted out from the right side of specimen holder 13, so
After biological sample is added, then promote specimen holder 13 in.Drawer cover 5 is additionally provided in specimen holder 13, such as Fig. 1-3, before streak inoculation,
Drawer cover 5 is in the left side of specimen holder 13, and two-sided 1 lower end inoculation hole of culture plate is not blocked, and two inoculation arms 31 can be worn
The inoculation hole for crossing two-sided 1 upper and lower ends of culture plate extend into drawer type sample pool 2, biological sample is dipped, then in culture tank 12
It crosses on interior culture medium;After the completion of streak inoculation, by the Slideslip to the right of drawer cover 5, drawer cover 5 is by two-sided 1 lower end of culture plate
Inoculation hole block, prevent bacterium from volatilizing.
What inoculator fixing seat 11 cooperated equipped with the card slot 15 being clamped with inoculator cross bar 33 and with inoculator gag lever post
Location hole facilitates the installation of inoculator 3 Yu inoculator fixing seat 11.Inoculator 3 is equipped with operation handle 32, and operation is facilitated to be inoculated with
Device 3.The lower end of inoculator 3 two inoculation arms 31 is equipped with two ranks buckle 34, and two ranks buckle 34 connects with two-sided 1 upper end of culture plate
The cooperation of kind hole, prevents inoculator 3 from skidding off.
In use, extraction drawer type sample pool 2, biological sample is added in drawer type sample pool 2, drawer style is closed
This pond 2;Inoculator 3 is inoculated with arm 31 by two and the oese of end is driven to extend into drawer type sample pool 2, dips biological sample
This;The operation handle 32 at 3 top of inoculator is operated, inoculation arm 31 drives the oese of end on the culture medium in culture tank 12
Scribing line, achievees the purpose that streak inoculation.
Basic principle and key structural feature of the invention has been shown and described above.The present invention is not by the limit of examples detailed above
System, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It both falls in the range of claimed invention.The claimed scope of the invention is by appended claims and its equivalent
It defines.
Claims (9)
- The culture apparatus 1. a kind of drawer type biological sample is crossed, including two-sided culture plate (1), two-sided culture plate (1) two sides It is equipped with the culture tank (12) for holding culture medium, it is characterised in that: two-sided culture plate (1) lower end is equipped with specimen holder (13), it is equipped with sliding slot in the specimen holder (13), the drawer type sample pool for holding biological sample is equipped in the sliding slot (2), the drawer type sample pool (2) is connected to the culture tank (12) on two-sided culture plate (1) two sides;The drawer type sample pool (2) it is inserted into from one end of specimen holder (13), separates the drawer covers (5) of the culture tank (12) and drawer type sample pool (2) from sample The other end of this seat (13) is inserted into.
- The culture apparatus 2. drawer type biological sample according to claim 1 is crossed, it is characterised in that: the two-sided culture plate (1) upper end is equipped with the inoculator fixing seat (11) for installing inoculator (3), and culture tank (12) setting is fixed in inoculator Between seat (11) and specimen holder (13), two inoculation arms (31) of the inoculator (3) sequentially pass through inoculator fixing seat (11) With culture tank (12), it extend into the drawer type sample pool (21) on specimen holder (2) Nei.
- The culture apparatus 3. drawer type biological sample according to claim 1 is crossed, it is characterised in that: the two-sided culture plate (1) two sides is equipped with the cover board (4) for covering culture tank (12).
- The culture apparatus 4. drawer type biological sample according to claim 3 is crossed, it is characterised in that: in the cover board (4) It is equipped with mutual corresponding convex ribs on side wall and two-sided culture plate (1) lateral wall, and is connected together by convex ribs fixation.
- The culture apparatus 5. drawer type biological sample according to claim 4 is crossed, it is characterised in that: on the cover board (4) Equipped with triangle limit plate (41), the inoculator fixing seat (11) is equipped with the limiting slot cooperated with triangle limit plate (41).
- The culture apparatus 6. drawer type biological sample according to claim 1 is crossed, it is characterised in that: the two-sided culture plate (1) it is made up of the identical two halves of structure the formation being clamped.
- The culture apparatus 7. drawer type biological sample according to claim 2 is crossed, it is characterised in that: the two-sided culture plate (1) upper and lower ends are equipped with the inoculation hole passed through for inoculation arm (31).
- The culture apparatus 8. drawer type biological sample according to claim 2 is crossed, it is characterised in that: the inoculator is fixed Seat (11) is equipped with the card slot (15) being clamped with inoculator cross bar (33) and the location hole with the cooperation of inoculator gag lever post.
- The culture apparatus 9. drawer type biological sample according to claim 2 is crossed, it is characterised in that: the inoculator (3) It is equipped with operation handle (32).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811623314.1A CN109554290B (en) | 2018-12-28 | 2018-12-28 | Drawer type biological sample streak culture apparatus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811623314.1A CN109554290B (en) | 2018-12-28 | 2018-12-28 | Drawer type biological sample streak culture apparatus |
Publications (2)
Publication Number | Publication Date |
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CN109554290A true CN109554290A (en) | 2019-04-02 |
CN109554290B CN109554290B (en) | 2024-04-12 |
Family
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CN201811623314.1A Active CN109554290B (en) | 2018-12-28 | 2018-12-28 | Drawer type biological sample streak culture apparatus |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN202007221U (en) * | 2011-01-17 | 2011-10-12 | 姜亚伟 | Plate for isolated culture of biological sample |
CN202390439U (en) * | 2011-12-22 | 2012-08-22 | 武汉迪艾斯科技有限公司 | Partition streak culture board of biological samples |
CN203333648U (en) * | 2013-06-28 | 2013-12-11 | 姜世民 | Separate culture device for biological samples |
CN207062281U (en) * | 2017-01-17 | 2018-03-02 | 迪艾斯医学(武汉)有限公司 | Sample smear can be carried out is separately cultured device |
CN209741165U (en) * | 2018-12-28 | 2019-12-06 | 武汉迪艾斯科技有限公司 | Drawer type biological sample lineation culture device |
-
2018
- 2018-12-28 CN CN201811623314.1A patent/CN109554290B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN202007221U (en) * | 2011-01-17 | 2011-10-12 | 姜亚伟 | Plate for isolated culture of biological sample |
CN202390439U (en) * | 2011-12-22 | 2012-08-22 | 武汉迪艾斯科技有限公司 | Partition streak culture board of biological samples |
CN203333648U (en) * | 2013-06-28 | 2013-12-11 | 姜世民 | Separate culture device for biological samples |
CN207062281U (en) * | 2017-01-17 | 2018-03-02 | 迪艾斯医学(武汉)有限公司 | Sample smear can be carried out is separately cultured device |
CN209741165U (en) * | 2018-12-28 | 2019-12-06 | 武汉迪艾斯科技有限公司 | Drawer type biological sample lineation culture device |
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