CN109554290B - Drawer type biological sample streak culture apparatus - Google Patents
Drawer type biological sample streak culture apparatus Download PDFInfo
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- CN109554290B CN109554290B CN201811623314.1A CN201811623314A CN109554290B CN 109554290 B CN109554290 B CN 109554290B CN 201811623314 A CN201811623314 A CN 201811623314A CN 109554290 B CN109554290 B CN 109554290B
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- inoculator
- sample
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- drawer type
- double
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- 239000012472 biological sample Substances 0.000 title claims abstract description 26
- 239000000523 sample Substances 0.000 claims abstract description 89
- 238000011081 inoculation Methods 0.000 claims abstract description 26
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
Abstract
The utility model relates to a drawer type biological sample streaking culture device. The culture device comprises a double-sided culture plate, a drawer type sample cell and an inoculator, wherein two inoculation arms of the inoculator sequentially penetrate through an inoculator fixing seat and a culture tank and extend into the drawer type sample cell of the sample seat; the two sides of the double-sided culture plate are both provided with inwardly concave culture tanks, a drawer type sample pool is arranged in a sample seat at the lower end of the double-sided culture plate, a biological sample is added into the drawer type sample pool by drawing out the drawer type sample pool, two inoculating arms of the inoculating device extend into the sample pool, the biological sample is dipped, the inoculating device is operated, and streaking inoculation is carried out on a culture medium contained in the culture tanks, so that the double-sided culture plate is convenient to use; one end of the culture plate is provided with an inoculator fixing seat for installing an inoculator, the other end of the culture plate is provided with a sample seat for installing a drawer type sample cell, and the culture tank is arranged between the inoculator fixing seat and the sample seat, so that the structure is simple and reasonable.
Description
Technical Field
The utility model relates to the technical field of microorganism culture detection, in particular to a drawer type biological sample streak culture device.
Background
In clinical and medical research experiments in hospitals, it is often necessary to separate, culture and smear samples of blood, sputum, feces, urine, body fluids, etc. of patients. The current common detection method is to manually pretreat the sample in the early stage, streak and inoculate the sample on a culture dish after the pretreatment is completed, and then manually transfer the culture dish into an incubator for culture. The series of operation processes have great disadvantages: because operators are extremely easy to contact with the sample under the condition, the infection is easy to cause, and the health of the operators is seriously threatened. In addition, during manual operation, since the above-mentioned sample also gives off unpleasant smell, it affects the analysis and the operation of an experimenter, thereby affecting the accuracy of the analysis result. The utility model patent of China patent No. 200520096773.0, biological sample separation and culture device, discloses a culture device for collecting biological samples and separating and culturing bacteria on the samples, which consists of two culture plates with bottom to bottom, a sample pool and an inoculator. The solid culture medium is filled on the culture plate, inoculators are arranged on two sides of the culture plate, the lower end of each inoculator is connected with an inoculating loop, the inoculating loop extends to a sample pool below the culture plate, and the upper end of each inoculator is connected with a driving handle. The isolated culture instrument of this structure has the disadvantages that: due to the structural characteristics, the sample pool is only used for storing samples, so that the inoculating loop is favorable for staining the samples for streaking inoculation, but the biological samples in the sample pool are smeared and stained, and the samples in the sample pool are required to be sampled and filled on a glass slide by a liquid transfer device for smearing, so that the sample pool is inconvenient. The smear is carried out on the sample in the sample pool so as to confirm that the infected bacteria belong to G+ or G-, so as to send a first-level report to clinic in time, which is an important link in the microorganism detection process.
Disclosure of Invention
In order to solve the problems, the utility model provides the drawer type biological sample partition streaking culture device which is simple in structure and convenient to use.
The technical scheme adopted by the utility model is as follows: the utility model provides a drawer type biological sample marking off culture apparatus, includes two-sided culture plate, two sides of two-sided culture plate all are equipped with the culture tank that is used for holding the culture medium, its characterized in that: the lower end of the double-sided culture plate is provided with a sample seat, a chute is arranged in the sample seat, a drawer type sample pool for containing biological samples is arranged in the chute, and the drawer type sample pool is communicated with culture grooves on two sides of the double-sided culture plate; the drawer type sample cell is inserted from one end of the sample holder, and a drawer cover separating the culture tank and the drawer type sample cell is inserted from the other end of the sample holder.
Preferably, the upper end of the double-sided culture plate is provided with an inoculator fixing seat for installing an inoculator, the culture tank is arranged between the inoculator fixing seat and the sample seat, and two inoculation arms of the inoculator sequentially penetrate through the inoculator fixing seat and the culture tank and extend into a drawer-type sample pool on the sample seat.
Preferably, the two sides of the double-sided culture plate are provided with cover plates for covering the culture tanks.
Preferably, the inner side wall of the cover plate and the outer side wall of the double-sided culture plate are respectively provided with convex ribs which correspond to each other, and are fixedly clamped together through the convex ribs.
Further, a triangular limiting plate is arranged on the cover plate, and a limiting groove matched with the triangular limiting plate is arranged on the inoculator fixing seat.
Preferably, the double-sided culture plate is formed by clamping two halves with the same structure.
Furthermore, the upper end and the lower end of the double-sided culture plate are respectively provided with an inoculation hole for the inoculation arm to pass through.
Further, the inoculator fixing seat is provided with a clamping groove clamped with the inoculator cross rod and a positioning hole matched with the inoculator limiting rod.
Further, an operating handle is arranged on the inoculator.
The beneficial effects obtained by the utility model are as follows: the two sides of the double-sided culture plate are both provided with inwardly concave culture tanks, a drawer type sample pool is arranged in a sample seat at the lower end of the double-sided culture plate, a biological sample is added into the drawer type sample pool by drawing out the drawer type sample pool, two inoculating arms of the inoculating device extend into the sample pool, the biological sample is dipped, the inoculating device is operated, and streaking inoculation is carried out on a culture medium contained in the culture tanks, so that the double-sided culture plate is convenient to use; one end of the culture plate is provided with an inoculator fixing seat for installing an inoculator, the other end of the culture plate is provided with a sample seat for installing a drawer type sample cell, and the culture tank is arranged between the inoculator fixing seat and the sample seat, so that the structure is simple and reasonable; the double-sided culture plate comprises two halves with the same structure and formed by clamping, the two sides of the double-sided culture plate are respectively provided with a cover plate for covering the culture tank, the cover plates are fixedly clamped with the culture plate through convex ribs, sliding grooves are respectively formed in the two sides of the sample seat fixing mechanism, sliding ribs matched with the sliding grooves are arranged on the inner side walls of the sample seat, and the double-sided culture plate is convenient to install and detach.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a state diagram of drawer type sample cell extraction;
fig. 3-4 are schematic diagrams of two different states of the present utility model.
Detailed Description
The utility model will be further described with reference to the drawings and the specific examples.
As shown in fig. 1-4, a drawer type biological sample streaking culture device comprises a double-sided culture plate 1, a drawer type sample cell 2 and an inoculator 3, wherein the double-sided culture plate 1 is vertically placed, an inoculator fixing seat 11 for installing the inoculator 3 is arranged at the upper end of the double-sided culture plate 1, a sample seat 13 for installing the drawer type sample cell 2 is arranged at the lower end (the drawer type sample cell 2 can be pushed in or pulled out in the sample seat 13), culture grooves 12 which are inwards concave are formed in two sides of the double-sided culture plate 1, the culture grooves 12 are integrally circular and are used for placing culture mediums, and the culture grooves 12 are arranged between the inoculator fixing seat 11 and the sample seat fixing mechanism 12. The inoculator 3 is of a fork structure, two inoculation arms 31 sequentially penetrate through the inoculator fixing seat 11 and the culture tank 12 from the inside of the double-sided culture plate 1, extend into the drawer type sample cell 2 of the sample seat 13, and are added with biological samples after being drawn out of the drawer type sample cell 2.
The two sides of the double-sided culture plate 1 are respectively provided with a cover plate 4 for covering the culture tank 12, the inner side wall of the cover plate 4 and the outer side wall of the double-sided culture plate 1 are respectively provided with convex ribs corresponding to each other, the cover plate 4 is pressed, and the double-sided culture plate 1 and the cover plate 4 are fixedly clamped together through the convex ribs. In this embodiment, the upper part of the cover plate 4 is provided with a triangular limiting plate 41, the inoculator fixing seat 11 is provided with a limiting groove matched with the triangular limiting plate 41, the circular part 42 of the cover plate 4 is matched with the circular culture groove 12, the circular part 42 of the cover plate 4 is provided with a hand-held part 43 protruding outwards, the triangular limiting plate 41 is matched with the limiting groove on the inoculator fixing seat 11, the positioning is accurate, and the inoculation arm 31 can smoothly extend into the sample pool 21; by providing the hand-held portion 43, the cover plate 4 is facilitated to be opened.
The double-sided culture plate 1 is formed by clamping two halves with the same structure, and the bottoms of the two half double-sided culture plates 1 are buckled and clamped together in a mode of protruding ribs and holes.
The two inner side surfaces of the sample seat 13 are respectively provided with a sliding groove, and the outer side wall of the drawer type sample cell 2 is provided with sliding ribs matched with the sliding grooves. Under the cooperation of the sliding groove and the sliding rib, the drawer type sample cell 2 can be pulled out from the right side of the sample seat 13, then the biological sample is added, and then the sample seat 13 is pushed in. The sample holder 13 is further provided with a drawer cover 5, as shown in fig. 1-3, before streaking inoculation, the drawer cover 5 is positioned on the left side of the sample holder 13, the seed holes at the lower end of the double-sided culture plate 1 are not plugged, two inoculation arms 31 can extend into the drawer type sample cell 2 through the seed holes at the upper end and the lower end of the double-sided culture plate 1, dip biological samples, and then streak on the culture medium in the culture tank 12; after streaking inoculation is completed, the drawer cover 5 slides to the right, and the inoculation holes at the lower end of the double-sided culture plate 1 are plugged by the drawer cover 5, so that bacteria are prevented from volatilizing.
The inoculator fixing seat 11 is provided with a clamping groove 15 clamped with the inoculator cross rod 33 and a positioning hole matched with the inoculator limiting rod, so that the inoculator 3 and the inoculator fixing seat 11 can be conveniently installed. The inoculator 3 is provided with an operating handle 32, which is convenient for operating the inoculator 3. The lower ends of two inoculation arms 31 of the inoculator 3 are respectively provided with a two-stage buckle 34, and the two-stage buckles 34 are matched with inoculation holes at the upper end of the double-sided culture plate 1 to prevent the inoculator 3 from sliding out.
When in use, the drawer type sample cell 2 is drawn out, a biological sample is added into the drawer type sample cell 2, and the drawer type sample cell 2 is closed; the inoculator 3 drives the inoculating loop at the tail end to extend into the drawer type sample pool 2 through two inoculating arms 31, and dips in biological samples; the operating handle 32 at the top of the inoculator 3 is operated, and the inoculating arm 31 drives the inoculating loop at the tail end to streak on the culture medium in the culture tank 12, so that the purpose of streaking inoculation is achieved.
The foregoing has shown and described the basic principles and main structural features of the present utility model. The present utility model is not limited to the above examples, and various changes and modifications may be made therein without departing from the spirit and scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (1)
1. The utility model provides a drawer type biological sample marking off culture apparatus, includes two-sided culture plate (1), two sides of two-sided culture plate (1) all are equipped with culture tank (12) that are used for holding the culture medium, its characterized in that: the lower end of the double-sided culture plate (1) is provided with a sample seat (13), a chute is arranged in the sample seat (13), a drawer type sample pool (2) for containing biological samples is arranged in the chute, and the drawer type sample pool (2) is communicated with culture grooves (12) on two sides of the double-sided culture plate (1); the drawer type sample cell (2) is inserted from one end of the sample seat (13), and a drawer cover (5) for separating the culture tank (12) from the drawer type sample cell (2) is inserted from the other end of the sample seat (13);
the upper end of the double-sided culture plate (1) is provided with an inoculator fixing seat (11) for installing an inoculator (3), the culture tank (12) is arranged between the inoculator fixing seat (11) and the sample seat (13), and two inoculation arms (31) of the inoculator (3) sequentially penetrate through the inoculator fixing seat (11) and the culture tank (12) and extend into a drawer-type sample pool (21) on the sample seat (2);
cover plates (4) for covering the culture tanks (12) are arranged on both sides of the double-sided culture plate (1); the inner side wall of the cover plate (4) and the outer side wall of the double-sided culture plate (1) are respectively provided with convex ribs corresponding to each other, and are fixedly clamped together through the convex ribs; a triangular limiting plate (41) is arranged on the cover plate (4), and a limiting groove matched with the triangular limiting plate (41) is arranged on the inoculator fixing seat (11); the double-sided culture plate (1) is formed by clamping two halves with the same structure; the upper end and the lower end of the double-sided culture plate (1) are respectively provided with an inoculation hole for an inoculation arm (31) to pass through; the inoculator fixing seat (11) is provided with a clamping groove (15) which is clamped with the inoculator cross bar (33) and a positioning hole which is matched with the inoculator limiting rod; an operating handle (32) is arranged on the inoculator (3);
the outer side wall of the drawer type sample cell (2) is provided with a sliding rib matched with the sliding groove, the drawer type sample cell (2) can be pulled out from the right side of the sample seat (13) under the matching of the sliding groove and the sliding rib, then a biological sample is added, and then the sample seat (13) is pushed in; a drawer cover (5) is further arranged on the sample seat (13), before streaking inoculation, the drawer cover (5) is positioned on the left side of the sample seat (13), the seed holes at the lower end of the double-sided culture plate (1) are not plugged, two inoculation arms (31) can penetrate through the seed holes at the upper end and the lower end of the double-sided culture plate (1) to extend into the drawer type sample tank (2), a biological sample is dipped, and then the culture medium in the culture tank (12) is streaked; after scribing inoculation is completed, the drawer cover (5) slides to the right, and the inoculation holes at the lower ends of the double-sided culture plates (1) are plugged by the drawer cover (5) to prevent bacteria from volatilizing;
the inoculator fixing seat (11) is provided with a clamping groove (15) which is clamped with the inoculator cross rod (33) and a positioning hole which is matched with the inoculator limiting rod, so that the inoculator (3) and the inoculator fixing seat (11) can be conveniently installed; an operating handle (32) is arranged on the inoculator (3), so that the inoculator (3) can be conveniently operated; the lower ends of the two inoculation arms (31) of the inoculator (3) are respectively provided with a two-stage buckle (34), and the two-stage buckles (34) are matched with inoculation holes at the upper ends of the double-sided culture plates (1) to prevent the inoculator (3) from sliding out;
when in use, the drawer type sample cell (2) is drawn out, the biological sample is added into the drawer type sample cell (2), and the drawer type sample cell (2) is closed; the inoculator (3) drives an inoculating loop at the tail end to extend into the drawer type sample pool (2) through two inoculating arms (31) to dip in a biological sample; an operating handle (32) at the top of the inoculator (3) is operated, and the inoculating arm (31) drives an inoculating loop at the tail end to streak on the culture medium in the culture tank (12), so that the purpose of streaking inoculation is achieved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811623314.1A CN109554290B (en) | 2018-12-28 | 2018-12-28 | Drawer type biological sample streak culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811623314.1A CN109554290B (en) | 2018-12-28 | 2018-12-28 | Drawer type biological sample streak culture apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109554290A CN109554290A (en) | 2019-04-02 |
| CN109554290B true CN109554290B (en) | 2024-04-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811623314.1A Active CN109554290B (en) | 2018-12-28 | 2018-12-28 | Drawer type biological sample streak culture apparatus |
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| Country | Link |
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| CN (1) | CN109554290B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202007221U (en) * | 2011-01-17 | 2011-10-12 | 姜亚伟 | Plate for isolated culture of biological sample |
| CN202390439U (en) * | 2011-12-22 | 2012-08-22 | 武汉迪艾斯科技有限公司 | Partition streak culture board of biological samples |
| CN203333648U (en) * | 2013-06-28 | 2013-12-11 | 姜世民 | Separate culture device for biological samples |
| CN207062281U (en) * | 2017-01-17 | 2018-03-02 | 迪艾斯医学（武汉）有限公司 | Sample smear can be carried out is separately cultured device |
| CN209741165U (en) * | 2018-12-28 | 2019-12-06 | 武汉迪艾斯科技有限公司 | Drawer type biological sample lineation culture device |
-
2018
- 2018-12-28 CN CN201811623314.1A patent/CN109554290B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202007221U (en) * | 2011-01-17 | 2011-10-12 | 姜亚伟 | Plate for isolated culture of biological sample |
| CN202390439U (en) * | 2011-12-22 | 2012-08-22 | 武汉迪艾斯科技有限公司 | Partition streak culture board of biological samples |
| CN203333648U (en) * | 2013-06-28 | 2013-12-11 | 姜世民 | Separate culture device for biological samples |
| CN207062281U (en) * | 2017-01-17 | 2018-03-02 | 迪艾斯医学（武汉）有限公司 | Sample smear can be carried out is separately cultured device |
| CN209741165U (en) * | 2018-12-28 | 2019-12-06 | 武汉迪艾斯科技有限公司 | Drawer type biological sample lineation culture device |
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| Publication number | Publication date |
|---|---|
| CN109554290A (en) | 2019-04-02 |
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