CN109929757A - A kind of novel nucleic acids extraction apparatus - Google Patents
A kind of novel nucleic acids extraction apparatus Download PDFInfo
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- CN109929757A CN109929757A CN201910361568.9A CN201910361568A CN109929757A CN 109929757 A CN109929757 A CN 109929757A CN 201910361568 A CN201910361568 A CN 201910361568A CN 109929757 A CN109929757 A CN 109929757A
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- 238000000605 extraction Methods 0.000 title claims abstract description 70
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 62
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 62
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 62
- 238000010438 heat treatment Methods 0.000 claims abstract description 46
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 27
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims description 78
- 230000005291 magnetic effect Effects 0.000 claims description 40
- 239000000463 material Substances 0.000 claims description 32
- 239000000758 substrate Substances 0.000 claims description 16
- 230000005540 biological transmission Effects 0.000 claims description 11
- 238000009434 installation Methods 0.000 claims description 11
- 238000005202 decontamination Methods 0.000 claims description 10
- 230000003588 decontaminative effect Effects 0.000 claims description 10
- 238000009826 distribution Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 12
- 238000000034 method Methods 0.000 description 22
- 230000008569 process Effects 0.000 description 15
- 239000011324 bead Substances 0.000 description 11
- 238000010586 diagram Methods 0.000 description 9
- 230000033001 locomotion Effects 0.000 description 7
- 238000010828 elution Methods 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 238000005336 cracking Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 230000005298 paramagnetic effect Effects 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 238000010276 construction Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003793 prenatal diagnosis Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000002601 radiography Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention is intended to provide a kind of novel nucleic acids extraction apparatus, including a pedestal, the station unit being set on the base, the extraction unit being arranged in above station unit, station unit includes a rotary components, the station platform rotated around rotary components, is arranged in several stations on station platform for placing reagent container and the rotation drive device of driving station platform rotation, and extraction unit includes the up-down drive device that a rack being arranged on the upside of rotary components, the agitating device that is arranged on the rack and magnetically attractive device and driving agitating device and magnetically attractive device move up and down.The present invention substantially increases experimental group number during one of nucleic acid extraction, nucleic acid extraction is greatly speeded up, simultaneously the device structure is compact, size is small, it is stable, be easy to monitor, heating speed it is fast, have in space layout and be significantly improved, using effect is excellent.
Description
Technical field
The present invention relates to a kind of novel nucleic acids extraction apparatus.
Background technique
With popularizing for genetic test, Personalized Drug Administration, pre-natal diagnosis etc., high pass is all pursued in each field of biological industry
Amount, the today automated, the limitation of traditional DNA extraction method is more and more obvious.It can be realized certainly since paramagnetic particle method extracts nucleic acid
Dynamicization extract, carry out high-volume operation, and it is easy to operate, the used time is short, thus paramagnetic particle method extract nucleic acid increasingly receive attention.
There is also some automatic nucleic acid extraction apparatus on the market, but structure is complicated mostly on the whole, extracts for these extraction apparatus
Process is not cumbersome, easy to operate.And the cracking of instrument for extracting nucleic acid, in conjunction with from elution etc. processes be in the different holes of the same deep-well plates
Middle progress, thus the experimental group quantity that can carry out nucleic acid extraction simultaneously is on the low side, it is partially slow so as to cause nucleic acid extraction speed, it cannot
Meets the needs of nucleic acid extraction.
And applicant once applied for that a Patent No. 201721928096.3, a kind of extraction of entitled Rapid nucleic acid were set a few days ago
Standby patent, although the patent solves multiple groups experimental group to a certain extent while being tested and can rapidly extracting nucleic acid
The problem of nucleic acid, but the equipment overall structure size is bigger than normal, space utilization rate is lower, operation exist vibration with it is unstable, no
It easily monitors, heat the problems such as slower.It is above-mentioned to solve that applicant devises this product on the basis of sustained improvement equipment
Problem has got good effect.
Summary of the invention
For the defect for overcoming the above-mentioned prior art, the embodiment of the invention provides a kind of novel nucleic acids extraction apparatus, the equipment
By using the cracking, combination, washing of different reagent container (deep-well plates) Lai Shixian paramagnetic particle methods during nucleic acid extraction
With the process such as elution, experimental group number is substantially increased during one of nucleic acid extraction, has greatly speeded up nucleic acid extraction,
Simultaneously the device structure is compact, size is small, it is stable, be easy to monitor, heating speed it is fast, in space layout have it is very big
It improves, using effect is excellent.
In order to achieve the above object, a kind of novel nucleic acids extraction apparatus is provided, including a pedestal, the station being set on the base
Unit, the extraction unit being arranged in above station unit, station unit includes a rotary components, the station that rotates around rotary components
Several stations on station platform for placing reagent container and the rotation drive device of driving station platform rotation is arranged in platform,
Extraction unit includes a rack being arranged on the upside of rotary components, the agitating device that is arranged on the rack and magnetically attractive device and drive
The up-down drive device that dynamic agitating device and magnetically attractive device move up and down.
Preferably, rotary components include one be fixed on pedestal rotating basis, be rotatably arranged on rotating basis
Station seat and frame seat for installation frame, the lower end of station seat be provided with a driving member, rotation drive device and pass
Moving part transmission connection, station platform are arranged in station seat upper end, and frame seat is fixedly connected with rotating basis.
Preferably, one is provided on rotating basis to be arranged on boss for limiting with the boss of load-bearing, station seat.
Preferably, the downside of frame seat is interconnecting piece, upside is mounting portion, and a use is provided between interconnecting piece and mounting portion
The limiting section limited on the upside of station seat, rack are mounted on mounting portion, and interconnecting piece is fixed after passing through station seat with rotating basis
Connection.
Preferably, agitating device includes puddle support, the stirring consumptive material being arranged on puddle support, and magnetically attractive device includes magnetic force
Frame, the magnetic bar being arranged on magnetic frame, stirring consumptive material includes several stirring rod corresponding with reagent container, and stirring rod axle center is set
It is equipped with the magnetically attractive blind hole of opening upwards, magnetic bar can be inserted into magnetically attractive blind hole, and up-down drive device includes that driving puddle support is transported up and down
The magnetically attractive driving device that dynamic mixer drive and driving magnetic frame moves up and down.
Preferably, up-down drive device further includes a transmission component being arranged on the rack, and transmission component includes that setting exists
The guide rail of rack side, the stirring sliding block being slidably arranged on guide rail and magnetically attractive sliding block, agitating device further include one and stirring cunning
The stirring seat of block connection, magnetically attractive device further includes a magnetically attractive seat connecting with magnetically attractive sliding block, and puddle support is mounted in stirring seat, magnetic
Power frame is mounted on magnetically attractive seat, and mixer drive is moved up and down by the first belt-driven stirring seat, magnetically attractive driving device
Magnetically attractive seat is driven to move up and down by the second transmission belt.
Preferably, gripper component is provided on puddle support, gripper component includes several grippers, a universal driving shaft and driving connection
The gripper driving device of moving axis rotation, gripper are arranged on universal driving shaft, and for gripper in hook-shaped, stirring consumptive material includes a substrate, stirring
Stick is disposed on the substrate, be provided on substrate with the matched crawl section of gripper, crawl section be an inverted U-shaped flange to raise upward,
Flange outer sides face is provided with the notch passed through for gripper, and the installation space passed through for flange is provided on puddle support.
Preferably, a heating device is provided with below the station below extraction unit, heating device includes a heating group
Part and driving heating component move up and down heater driver, heating component include one with the matched heating plate of reagent container and
One, for installing the heated seats of heating plate, is evenly arranged with heating source, heating back is evenly arranged with several use in heating plate
In the heating installation position of installation heating source, heating plate includes the bottoming hole of several array distributions, between four adjacent bottoming holes
It is provided with a heated beam protruding upward, there are four the arcuation sides to match with bottoming hole for heated beam tool.
Preferably, instrument for extracting nucleic acid further includes a decontamination system, decontamination system include be set on the base it is several
Venthole and the negative pressure source being arranged on venthole.
Preferably, the balanced component of a stress for balancing agitating device and magnetically attractive device, balance are provided in rack
Component includes that the positive balance raised line of rack, the magnetically attractive balance weight being slidably arranged on balance raised line and stirring balance is arranged in
Block, magnetically attractive balance weight and stirring balance weight are connect with magnetically attractive device and agitating device close to the positive side of rack respectively.
The present invention compared with prior art the advantages of are as follows:
The present invention is during nucleic acid extraction by using different reagent container (deep-well plates) Lai Shixian paramagnetic particle methods
The processes such as cracking, combination, washing and elution substantially increase experimental group number during one of nucleic acid extraction, greatly
Accelerate nucleic acid extraction, at the same the device structure is compact, size is small, it is stable, be easy to monitor, heating speed it is fast, in space
Tool is significantly improved in layout, and using effect is excellent.
The position of station platform rotary shaft has creatively been arranged in by the setting of rotary components in rack by this product,
Rotary components are able to maintain that rack is fixed when realizing the rotation of station platform, improve the space structure layout of product, greatly
The space structure and size of product are reduced, while more optimizing the process of nucleic acid extraction, so that the work of nucleic acid extraction process
Position setting improves to some extent with layout, and the placement of station reagent container can be completed by only needing station platform to turn around, and can immediately start
Extraction process no longer needs to relocate.
The balanced component of this equipment setting can balance the stress of agitating device and magnetically attractive device well, so that stirring dress
It sets and keeps stress balance during whipping process, magnetically attractive device are in magnetically attractive, realize the flat of agitating device and magnetically attractive device
Weighing apparatus property, stability reduce vibration and noise in operational process and avoid damage and strain.
The unique texture of heating plate enables reagent container more to protrude into bottoming hole, and the heated beam protruded has into one
Step increases the contact area with reagent container, greatly increases the heating speed to reagent container.
The venthole and negative pressure source of decontamination system are set on the base, can be in nucleic acid extraction when decontamination system starting
A top-down air flowing is formed inside instrument, enable the waste liquid exhaust gas generated during nucleic acid extraction in time to
Lower discharge avoids waste liquid exhaust gas from mixing with the sample in reagent container and pollutes, and can reduce because air flowing caused by
Sample volatilization.
Detailed description of the invention
Fig. 1 is overall structure structural schematic diagram of the invention；
Fig. 2 is the structural schematic diagram of extraction unit；
Fig. 3 is the decomposition texture schematic diagram of rotary components；
Fig. 4 is the structural schematic diagram for stirring consumptive material；
Fig. 5 is the structural schematic diagram of puddle support Yu gripper component；
Fig. 6 is the structural schematic diagram of gripper component；
Fig. 7 is the structural schematic diagram of heating component；
Fig. 8 is the structure enlargement diagram in Fig. 7 at A；
Fig. 9 is the structural schematic diagram for heating back.
Specific embodiment
In the present description, the orientation or positional relationship of the instructions such as term " on ", "lower", " preceding " and " rear " is based on attached
Orientation or positional relationship shown in figure is merely for convenience of the description present invention rather than requires the present invention must be with specific orientation
Construction and operation, therefore be not considered as limiting the invention.
Specific embodiments of the present invention will be further explained with reference to the accompanying drawing.
Referring to Fig.1-3, a kind of novel nucleic acids extraction apparatus, including a pedestal 1, the station unit being arranged on pedestal 1, setting
Extraction unit above station unit, station unit include a rotary components 2, around rotary components 2 rotate station platform 3, set
The rotation drive device that several stations 31 and the driving station platform 3 on station platform 3 for placing reagent container 32 rotate is set,
Extraction unit include one be arranged in rack 4, the agitating device being arranged in rack 4 and the magnetically attractive device of the upside of rotary components 2 with
And the up-down drive device that driving agitating device and magnetically attractive device move up and down.
Preferably, reagent container 32 is the deep-well plates in 96 holes or 24 holes, and station platform 3 is a turntable, on station platform 3
8 stations 31 are uniformly arranged, are provided with the mounting structure to match with deep-well plates at each station 31, deep-well plates are placed in station
At 31, and hollow out at the station 31 of placement deep-well plates, deep-well plates are interior to be pre-loaded with different reagents, 8 works according to requirement of experiment
Deep-well plates on position 31 include sample deep-well plates, the deep-well plates for containing magnetic bead, washing deep-well plates, elution deep-well plates etc..Certainly, root
According to requirement of experiment, the quantity of station 31 can be changed accordingly or using different deep-well plates.Rotary components 2 include one solid
The rotating basis 21 being arranged on pedestal 1 calmly, the station seat 22 that is rotatably arranged on rotating basis 21 and for installation frame 4
Frame seat 23, one is provided on rotating basis 21 for limit and the boss 25 of load-bearing, the setting of station seat 22 on boss 25,
Station platform 3 is arranged in 22 upper end of station seat, and the lower end of station seat 22 is provided with a driving member 24, rotation drive device and driving member
24 transmission connections drive the rotation of station seat 22 to drive station platform 3 to rotate, and the downside of frame seat 23 is interconnecting piece 27, upside is
Mounting portion 26, is provided with a limiting section 28 for limiting on the upside of station seat 22 between interconnecting piece 27 and mounting portion 26, rack 4 is pacified
On mounting portion 26, interconnecting piece 27 is fixedly connected after passing through station seat 22 with rotating basis 21.
This product passes through the setting of rotary components 2, and rack 4 has creatively been arranged in the position of 3 rotary shaft of station platform,
It is able to maintain that rack 4 is fixed when rotary components 2 realize the rotation of station platform 3, improves the space structure layout of product, greatly
The earth reduces the space structure and size of product, while more optimizing the process of nucleic acid extraction, so that nucleic acid extraction process
Station 31 setting improve to some extent with layout, the placement of 31 reagent container 32 of station, nothing can be completed by only needing station platform 3 to turn around
Need to rotate could repeatedly place deep-well plates according to nucleic acid extraction process, and can immediately start extraction process, no longer need to relocate.
When nucleic acid extraction, extraction unit is fixed in situ, according to experimentation, drives station platform by rotation drive device
3 rotations are turned between the deep-well plates on different station 31 using the deep-well plates on different station 31 to complete magnetic bead and sample
It moves, to complete the process of nucleic acid extraction.The present invention is during nucleic acid extraction by using different 32 (deep holes of reagent container
Plate) Lai Shixian paramagnetic particle method the processes such as cracking, combination, washing and elution, and instrument for extracting nucleic acid is completely different on the market at present,
One of nucleic acid extraction substantially increases experimental group number in the process, has greatly speeded up nucleic acid extraction, and device structure letter
Single, novel, simple to operate, nucleic acid extraction effect is good.
Referring to Figures 1 and 2, preferably, one is provided in rack 4 for balancing agitating device and magnetically attractive device
The balanced component of stress, balanced component include being arranged in the positive balance raised line 51 of rack 4, being slidably arranged on balance raised line 51
Magnetically attractive balance weight 52 and stirring balance weight 53, magnetically attractive balance weight 52 and stirring balance weight 53 respectively with magnetically attractive device and stir dress
Rest against the connection of nearly rack 4 positive side.Balanced component changes suction by the stress point of increase magnetically attractive device and agitating device
The distribution of force of magnetic device and agitating device can balance the stress of agitating device and magnetically attractive device well, so that stirring dress
It sets and keeps stress balance during whipping process, magnetically attractive device are in magnetically attractive, realize the flat of agitating device and magnetically attractive device
Weighing apparatus property, stability reduce vibration and noise in operational process and avoid damage and strain.
Referring to Fig. 7-9, preferably, the lower section of the station 31 below extraction unit is provided with a heating device, adds
Thermal includes the heater driver that a heating component 9 and driving heating component 9 move up and down, heating component 9 include one with
The matched heating plate of reagent container 32 92 and one for installing the heated seats 91 of heating plate 92, be evenly arranged in heating plate 92 plus
Heat source, 92 back side of heating plate are evenly arranged with several for installing the heating installation position 95 of heating source, heating installation position 95 preferably 3
A, heating plate 92 includes the bottoming hole 93 of several array distributions, and it is protruding upward to be provided with one between four adjacent bottoming holes 93
Heated beam 94, there are four the arcuation sides to match with bottoming hole 93 for the tool of heated beam 94.The unique texture of heating plate 92 to try
Agent container 32 can more protrude into bottoming hole 93, and the heated beam 94 protruded has to further increase and connects with reagent container 32
Contacting surface product, greatly increases the heating speed to reagent container 32.
Referring to Fig.1 and Fig. 4-6, preferably, agitating device includes puddle support 71, is arranged on puddle support 71
Consumptive material 72 is stirred, magnetically attractive device includes magnetic frame 61, the magnetic bar being arranged on magnetic frame 61 62, and stirring consumptive material 72 includes several
Stirring rod corresponding with reagent container 32, stirring rod axle center are provided with the magnetically attractive blind hole of opening upwards, and magnetic bar 62, which can be inserted into, to be inhaled
Magnetic blind hole, up-down drive device include the mixer drive 85 for driving puddle support 71 to move up and down and about 61 magnetic frame of driving
The magnetically attractive driving device 82 of movement.
During nucleic acid extraction, mixer drive 85 drives the decline of puddle support 71 that stirring consumptive material 72 is driven to be inserted into deep-well plates
The inside, magnetically attractive driving device 82 drive the decline of magnetic frame 61 to drive in the magnetically attractive blind hole of the insertion stirring consumptive material 72 of magnetic bar 62, from
And draw the magnetic bead being located in test solution.Stirring consumptive material 72 is driven upside down in deep-well plates on repeatedly by mixer drive 85
Lower movement and complete to the stirring of reagent and sample and uniformly, use, inhale during cracking, combination, washing, elution etc.
Magnetic device draws magnetic bead mainly during magnetic bead shifts, i.e., magnetic bead is from a deep-well plates into another deep-well plates.
Up-down drive device further includes a transmission component being arranged in rack 4, and transmission component includes being arranged in 4 side of rack
The guide rail 81 in face, the stirring sliding block 86 being slidably arranged on guide rail 81 and magnetically attractive sliding block 83, agitating device further include one and stirring
The stirring seat 73 that sliding block 86 connects, magnetically attractive device further includes a magnetically attractive seat 63 connecting with magnetically attractive sliding block 83, and puddle support is mounted on
In stirring seat 73, magnetic frame is mounted on magnetically attractive seat 63, and mixer drive 85 passes through in the first belt-driven stirring seat 73
Lower movement, magnetically attractive driving device drive magnetically attractive seat 63 to move up and down by the second transmission belt.In order to further be precisely controlled stirring
The movement of seat 73 and magnetically attractive seat 63 is preferably provided with a stirring origin switch 87 in stirring seat 73, on magnetically attractive seat 63
It is provided with a magnetically attractive origin switch 84.
Because needing agitating device during nucleic acid extraction and magnetically attractive device being opposing stationary moves up and down in overall, thus
Instrument for extracting nucleic acid on the market is usually that magnetically attractive device and magnetically attractive driving device are arranged on agitating device at present, is then led to
Mixer drive is crossed to drive agitating device to move up and down while realizing the opposing stationary of agitating device and magnetically attractive device.But it should
It is excessive that structure results in mixer drive weight bearing, it is difficult to and it realizes that agitating device quickly moves up and down, affects mixing effect,
And causes to bring biggish vibration and radiography in whipping process, simultaneously as magnetically attractive device is arranged on agitating device, cause
The magnetic frame of magnetically attractive device is shorter with respect to the up and down motion stroke of agitating device, and then results in instrument for extracting nucleic acid and may be only available for
The shorter situation of deep-well plates, magnetic bar, stirring rod, limits the scope of application of instrument for extracting nucleic acid.
And agitating device and magnetically attractive device are mutually separately separated setting by the present invention, greatly reduce mixer drive
85 load improves mixing effect, reduces stirring noise and vibration to improve the frequency that stirring rod stirs up and down,
The stroke for substantially increasing magnetically attractive device simultaneously, so that product of the present invention can be suitably used for the bigger orifice plate of size range, magnetic force
Stick 62, stirring rod expand the scope of application of instrument for extracting nucleic acid.
When that need to carry out magnetic bead transfer, magnetically attractive driving device drives the magnetically attractive blind hole of the insertion stirring consumptive material 72 of magnetic bar 62
In, so that magnetic bead to be drawn to the outer surface of stirring consumptive material 72, then magnetically attractive driving device and mixer drive 85 drive simultaneously
Dynamic magnetically attractive device and agitating device move upwards disengaging deep-well plates in the state of keeping opposing stationary, are attracted on to ensure
Magnetic bead on stirring consumptive material 72 does not fall off from the outer surface of stirring consumptive material 72, the effect for ensureing the complete of nucleic acid extraction and extracting
With efficiency.It is driven by conveyer belt, is conducive to magnetic bar 62 and stirs the steady and smooth up and down motion of consumptive material 72, and guide rail
81, the setting of magnetically attractive sliding block 83 and stirring sliding block 86 can not only ensure the steady, accurate of movement, moreover it is possible to further balance stirring dress
The stress with magnetically attractive device is set, better counterbalance effect is realized in the cooperation with balanced component.
Gripper component is provided on puddle support 71, gripper component is provided with two groups, is distributed in the two sides of puddle support 71, gripper
Component includes the gripper driving device 75 that several grippers 77, a universal driving shaft 78 and driving universal driving shaft 78 rotate, and gripper 77 is arranged
On universal driving shaft 78, for gripper 77 in hook-shaped, gripper driving device 75 is motor.Stirring consumptive material 72 includes a substrate 73, stirring rod
It is arranged on substrate 73, is provided on substrate 73 and the matched crawl section of gripper 77.Crawl section is provided with 4, is distributed in substrate
73 4 corners, crawl section are the inverted U-shaped flange 74 to raise upward, and 74 lateral surface of flange is provided with to be passed through for gripper 77
Notch is provided with the installation space 76 passed through for flange 74 on puddle support 71.Stirring rod array distribution is in the downside of substrate 73, base
Through-hole corresponding with magnetically attractive blind hole is provided on plate 73 and puddle support 71, so that the magnetic in the magnetically attractive device above agitating device
Power stick 62 enters in stirring rod, to complete the transfer of magnetic bead.
There are two grippers 77 for setting on one universal driving shaft 78, and are distributed in the two sides of universal driving shaft 78, are provided with two groups of grippers
The synchronism of gripper 77, while 4 grippers 77 and substrate has been better achieved in component on the lesser spatial position of puddle support 71
The crawl section in 73 corners matches, and grabs 4 corners of stirring consumptive material 72 when crawl simultaneously, so that stirring consumptive material 72
Substrate 73, which synchronizes, to be resisted against on puddle support 71, and 73 uniform force of substrate, and hook-shaped gripper 77 is easier to crawl substrate 73
Crawl section, and flange 74 is made to pass through installation space 76, so that stirring consumptive material 72 is closely combined with puddle support 71, when stirring consumes
Material 72 need to only lock gripper driving device 75 and can ensure puddle support 71 and stir the combination of consumptive material 72 after being combined with puddle support 71
Effect, to ensure the mixing effect of agitating device.When needing to separate puddle support 71 and stirring consumptive material 72, releases gripper and drive
The lock state of dynamic device 75 inverts gripper driving device 75.Gripper modular construction is simple, small and exquisite, can be quickly square
Just realize puddle support 71 and stir consumptive material 72 combination with separate, and puddle support 71 with stir being firmly combined for consumptive material 72 can
It leans on, has ensured the quality of nucleic acid extraction, while reducing the workload of experimenter and simplifying workflow.
When experiment starts, stirring consumptive material 72 need to be only placed in the deep-well plates of nucleic acid extraction, after experiment starts, deep hole
Plate is sent to 71 lower section of puddle support, and puddle support 71 moves to 72 top of stirring consumptive material, and the gripper 77 of gripper component grabs substrate 73
On crawl section, and by stirring consumptive material 72 be fixed on puddle support 71, thus realize puddle support 71 with stirring consumptive material 72 combination；
After nucleic acid extraction, it is the separation for realizing stirring consumptive material 72 and puddle support 71 that gripper 77, which unclamps stirring consumptive material 72,.
Referring to Fig.1, preferably, the invention also includes a decontamination system, decontamination system includes being arranged the bottom of at
Several ventholes on seat 1 and the negative pressure source 14 that is arranged on venthole, are provided with 4 as most preferred embodiment venthole,
It is separately positioned on the edge of pedestal 1,1 negative pressure source 14 is provided in each gas outlet, negative pressure source 14 is preferably negative pressure fan.
The venthole and negative pressure source 14 of decontamination system are set on the base, can be inside instrument for extracting nucleic acid when decontamination system starting
A top-down air flowing is formed, the waste liquid exhaust gas that nucleic acid extraction is generated in the process is arranged downwards in time
Out, avoid waste liquid exhaust gas from mixing with the sample in reagent container to pollute, and can reduce because air flowing caused by sample
Volatilization.
Further, decontamination system further includes an isolation board 11 that 3 downside of station platform is arranged in and is arranged in pedestal 1
On be used to support the support frame 13 of isolation board 11, in the corresponding place of station 31 of 14 upside of negative pressure source, isolation board 11 is arranged
There is honeycomb hole 12.Isolation board 11 can preferably guide the flow direction of air, improve antipollution effect, while isolation board 11 can be every
From the reagent to drip from station platform 3, to play the role of protective separation to the mechanism of 3 downside of station platform.In addition, isolation board 11
The internal structure that extraction apparatus can also be covered improves the visual effect of extraction apparatus, improves aesthetics.
In addition, preferably, a liquid-leakage preventing component can also be arranged in extraction unit, liquid-leakage preventing component can hide including one
Keep off the liquid-leakage preventing plate of agitating device lower space.The side of extraction unit is arranged in liquid-leakage preventing component, and liquid-leakage preventing plate is arranged one
On pivoted arm, when rotation drive device rotation station platform 3, pivoted arm rotation send liquid-leakage preventing plate to agitating device, prevents from stirring
It mixes the reagent on device, magnetic bead and sample etc. to be dropped in equipment, avoids damage equipment.And after station 31 switches,
Pivoted arm drive liquid-leakage preventing plate leaves the lower section of agitating device.
According to the disclosure and teachings of the above specification, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and is modified.Therefore, the invention is not limited to the specific embodiments disclosed and described above, to of the invention
Some modifications and changes should also be as falling into the scope of the claims of the present invention.In addition, although being used in this specification
Some specific terms, these terms are merely for convenience of description, does not limit the present invention in any way.
Claims (10)
1. a kind of novel nucleic acids extraction apparatus, it is characterised in that: it includes a pedestal, the station unit that is set on the base, setting
Extraction unit above station unit, the station unit include a rotary components, the station platform rotated around rotary components, set
Set several stations on station platform for placing reagent container and the rotation drive device of the driving station platform rotation, institute
Stating extraction unit includes a rack being arranged on the upside of the rotary components, the agitating device that is arranged in the rack and magnetically attractive
The up-down drive device that device and the driving agitating device and magnetically attractive device move up and down.
2. novel nucleic acids extraction apparatus as described in claim 1, it is characterised in that: the rotary components are fixed at including one
Rotating basis on pedestal, the station seat being rotatably arranged on rotating basis and the frame seat for installing the rack, institute
The lower end for stating station seat is provided with a driving member, and the rotation drive device and driving member are sequentially connected, the station platform setting
In station seat upper end, the frame seat is fixedly connected with the rotating basis.
3. novel nucleic acids extraction apparatus as claimed in claim 2, it is characterised in that: be provided with one on the rotating basis for limiting
The boss of position and load-bearing, the station seat are arranged on the boss.
4. novel nucleic acids extraction apparatus as claimed in claim 2, it is characterised in that: the downside of the frame seat be interconnecting piece, on
Side is mounting portion, and a limiting section for limiting on the upside of the station seat is provided between the interconnecting piece and mounting portion, described
Rack is mounted on the mounting portion, and the interconnecting piece is fixedly connected after passing through the station seat with the rotating basis.
5. novel nucleic acids extraction apparatus as described in claim 1, it is characterised in that: the agitating device includes puddle support, setting
Stirring consumptive material on puddle support, the magnetically attractive device includes magnetic frame, the magnetic bar that is arranged on magnetic frame, the stirring consumption
Material includes several stirring rod corresponding with reagent container, and the stirring rod axle center is provided with the magnetically attractive blind hole of opening upwards, described
Magnetic bar can be inserted into the magnetically attractive blind hole, and the up-down drive device includes the mixer drive for driving puddle support to move up and down
The magnetically attractive driving device to move up and down with driving magnetic frame.
6. novel nucleic acids extraction apparatus as claimed in claim 5, it is characterised in that: the up-down drive device further includes a setting
Transmission component in the rack, the transmission component include the guide rail that rack side is arranged in, are slidably arranged in described lead
Stirring sliding block and magnetically attractive sliding block on rail, the agitating device further includes a stirring seat connecting with the stirring sliding block, described
Magnetically attractive device further includes a magnetically attractive seat connecting with the magnetically attractive sliding block, and the puddle support is mounted in the stirring seat, described
Magnetic frame is mounted on the magnetically attractive seat, and the mixer drive is transported up and down by stirring seat described in the first belt-driven
Dynamic, the magnetically attractive driving device drives the magnetically attractive seat to move up and down by the second transmission belt.
7. novel nucleic acids extraction apparatus as claimed in claim 5, it is characterised in that: gripper component is provided on the puddle support,
The gripper component includes the gripper driving device of several grippers, a universal driving shaft and driving universal driving shaft rotation, and the gripper is set
It sets on universal driving shaft, for gripper in hook-shaped, the stirring consumptive material includes a substrate, and the stirring rod is arranged on the substrate, institute
State be provided on substrate with the matched crawl section of the gripper, the crawl section be an inverted U-shaped flange to raise upward, it is described convex
Edge lateral surface is provided with the notch passed through for the gripper, and it is empty that the installation passed through for the flange is provided on the puddle support
Between.
8. novel nucleic acids extraction apparatus as described in claim 1, it is characterised in that: the lower section of the station below the extraction unit
It is provided with a heating device, heating device includes the heater driver that a heating component and driving heating component move up and down,
The heating component includes the heated seats for being used to install heating plate with the matched heating plate of the reagent container and one, described to add
Heating source is evenly arranged on hot plate, heating back is evenly arranged with several for installing the heating installation position of heating source, institute
The bottoming hole that heating plate includes several array distributions is stated, a heated beam protruding upward is provided between four adjacent bottoming holes,
There are four the arcuation sides to match with bottoming hole for the heated beam tool.
9. novel nucleic acids extraction apparatus as described in claim 1, it is characterised in that: it further includes a decontamination system, described anti-
Contaminated system includes several ventholes being arranged on the base and the negative pressure source being arranged on venthole.
10. such as the described in any item novel nucleic acids extraction apparatus of claim 1-9, it is characterised in that: be provided with one in the rack
For balancing the balanced component of the stress of the agitating device and magnetically attractive device, the balanced component includes setting in rack front
Balance raised line, the magnetically attractive balance weight and stirring balance weight that are slidably arranged on balance raised line, the magnetically attractive balance weight and stirring
Balance weight is connect with the magnetically attractive device and agitating device close to the positive side of rack respectively.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112046988A (en) * | 2020-09-10 | 2020-12-08 | 成都纺织高等专科学校 | Biological sample refrigeration storage system |
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| CN109929757B (en) | 2024-03-22 |
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