CN107929693A - A kind of Chinese medicine microorganism formulation for treating nan yang yellow cattle inflatable and preparation method - Google Patents
A kind of Chinese medicine microorganism formulation for treating nan yang yellow cattle inflatable and preparation method Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine microorganism formulation for treating nan yang yellow cattle inflatable and preparation method, its raw material includes betel nut, Cortex Magnoliae Officinalis, rhizoma atractylodis, radix glycyrrhizae, Radix Codonopsis, rhizoma zingiberis, Rhizoma Atractylodis Macrocephalae, malt, Fructus Aurantii, aspergillus oryzae culture dried object, calf Bacillus acidi lactici culture dried object.Traditional Chinese medicine ingredients therein have effects that warming middle-JIAO and strengthening the spleen stomach invigorating, interior cold of dispelling, solution moisture, ejectment stagnant gas, are conducive to be vented；And aspergillus oryzae therein can then produce abundant protease, lipase, cellulase etc., nan yang yellow cattle can be helped effectively to decompose feed ingredient in stomach；Calf lactobacillus can be by adjusting gut pH, and promotes gastrointestinal peristalsis, helps nan yang yellow cattle to digest and assimilate food, mitigates flatulence, and the digestive enzymes such as its invertase produced, lactase, peptase also contributes to improve the digestive function of nan yang yellow cattle.When treating nan yang yellow cattle inflatable symptom using said preparation, Chinese medicine and microorganism act synergistically on nan yang yellow cattle spleen, stomach, intestines position, evident in efficacy.
Description
Technical field
The present invention relates to the technical field of livestock culturing, and in particular to a kind of Chinese medicine microorganism for treating nan yang yellow cattle inflatable
Preparation and preparation method.
Background technology
Nan yang yellow cattle is one of yellow cattle breed excellent greatly of China five, is distributed mainly on Nanyang City, Henan Province Tanghe, Baihe stream
The vast plains region in domain, its physique is tall and big, muscular, compact-sized, thin skin capillary, and rapid, the big upright, shoulder of mouth of taking action
Portion is generous, back of the body waist is straight, four limbs are rectified, and belongs to large-scale labour meat dual-purpose kind.Nan yang yellow cattle no matter male and female, be all good at walking, draw
Car, farming is rapid, there is the title of fast ox, and working capacity is very strong, in general, 2,3 mu of the daily arable land of nan yang yellow cattle, load-carrying 1200
36 kilometers or so of kilogram, day row, is the common farming of local farmers and means of transport.However, in busy farming season, if manager
Pay no heed to, it is easy to excessive forced labour nan yang yellow cattle, and feeding is eager to supplement its muscle power after forced labour；If manager raises
It is improper to support, and nan yang yellow cattle is easy to occur the symptom of inflatable.In addition, if the feed of the gluttonous excessive aerogenesis easy to ferment of ox also holds
Inflatable easily occurs.In addition, when exchanging autumn and winter, weather is easily mutated, attacked by the cold and evil, and feed is more easy to ice during feeding
Cold, cold air is easily condensed in nan yang yellow cattle taste, causes its operating imbalance that inflatable occurs.
At present, for the symptom of ox inflatable, Western medicine therapy is used mostly, for example, Lysol, nux vomica tincture is used in combination
Either also some ox managers are fed using acupuncture and moxibustion treatment or by gavaging atoleine alleviation using sodium thiosulfate
Inflatable symptom.In addition, also have some Chinese herbal medicine folk prescription treatment ox inflatables among the people, but its effect needs to be investigated.
However, had not been reported using Chinese medicine microorganism formulation treatment nan yang yellow cattle inflatable.
The content of the invention
The technical problems to be solved by the invention are to provide the medicine of another effectively treatment nan yang yellow cattle inflatable.
In order to solve the above technical problems, the present invention provides a kind of Chinese medicine microorganism formulation for treating nan yang yellow cattle inflatable,
Its raw material includes betel nut, Cortex Magnoliae Officinalis, rhizoma atractylodis, radix glycyrrhizae, Radix Codonopsis, rhizoma zingiberis, Rhizoma Atractylodis Macrocephalae, malt, Fructus Aurantii, aspergillus oryzae culture dried object, calf
Bacillus acidi lactici culture dried object.
Wherein, the parts by weight of each raw material are respectively betel nut 10-15 parts by weight, Cortex Magnoliae Officinalis 25-30 parts by weight, rhizoma atractylodis 15-
20 parts by weight, radix glycyrrhizae 5-10 parts by weight, Radix Codonopsis 20-25 parts by weight, rhizoma zingiberis 20-30 parts by weight, Rhizoma Atractylodis Macrocephalae 30-35 parts by weight, malt
10-15 parts by weight, Fructus Aurantii 30-35 parts by weight, aspergillus oryzae culture dried object 15-20 parts by weight, calf Bacillus acidi lactici culture drying
Thing 25-35 parts by weight.
Wherein, the preparation method of the aspergillus oryzae culture dried object is as follows：
Aspergillus oryzae strain is activated using PDA culture medium, then expands culture aspergillus oryzae, is collected by centrifugation after thalline and bacterium is made is hanged
Liquid, dispenses to serum bottle, and aspergillus oryzae culture dried object is obtained by the way of vacuum freeze drying, and low temperature is protected after nitrogen-filled seal
Hide, the used time takes.
Wherein, the preparation method of the calf Bacillus acidi lactici culture dried object is as follows：
Calf Lactobacillus species are activated using MRS culture mediums, then expand culture calf Bacillus acidi lactici, thalline is collected by centrifugation
After bacteria suspension is made, mannitol is added in bacteria suspension, then dispense to serum bottle, is obtained by the way of vacuum freeze drying small
Cow's milk acidfast bacilli culture dried object, low-temperature preservation after nitrogen-filled seal, used time take.
Present invention also offers the preparation method of above-mentioned Chinese medicine microorganism formulation：
The betel nut, Cortex Magnoliae Officinalis, rhizoma atractylodis, radix glycyrrhizae, Radix Codonopsis, rhizoma zingiberis, Rhizoma Atractylodis Macrocephalae, malt, Fructus Aurantii are mixed by weight, shred big gun
System, the mixture after processing is placed in porcelain, pours into white wine to being totally submerged mixture, closed, stirring 1 in every 2 days first 10 days
It is secondary, stir weekly once within latter 35 days, dipping takes out press residue after supernatant after 45 days, press juice is mixed with supernatant, dense
Contracting drying for standby；Aspergillus oryzae culture dried object and the calf Bacillus acidi lactici culture drying of corresponding parts by weight are added before feeding
Thing, up to Chinese medicine microorganism formulation.
The present invention is for a kind of Chinese medicine microorganism formulation for treating nan yang yellow cattle inflatable and preparation method is disclosed, wherein wrapping
Include Chinese herbal medicine, aspergillus oryzae and calf lactobacillus.The betel nut of said preparation use, rhizoma atractylodis, malt, Fructus Aurantii have warming middle-JIAO and strengthening the spleen stomach invigorating
Effect；The hot effect with interior cold of dispelling of rhizoma zingiberis, and Radix Codonopsis one work and are more advantageous to the taste that shake；Cortex Magnoliae Officinalis, Rhizoma Atractylodis Macrocephalae can be dissolved
Moisture, ejectment stagnant gas, are conducive to inflatable patient exhaust；And radix glycyrrhizae can reconcile each traditional Chinese medicine ingredients in said preparation.In addition, the system
Aspergillus oryzae in agent can produce abundant protease, lipase, cellulase etc., nan yang yellow cattle can be helped effectively to decompose intestines
Feed ingredient in stomach；And calf lactobacillus can be by adjusting gut pH, and promote gastrointestinal peristalsis, help nan yang yellow cattle digestion
Assimilate food, mitigate flatulence, the digestive enzymes such as its invertase produced, lactase, peptase also contributes to improve nan yang yellow cattle
Digestive function.When treating nan yang yellow cattle inflatable symptom using said preparation, Chinese medicine therein and microorganism act synergistically on Nanyang Huang
Ox spleen, stomach, intestines position, it is evident in efficacy.
Embodiment
The present invention is described further below, to make those skilled in the art to implement accordingly.
A kind of Chinese medicine microorganism formulation for treating nan yang yellow cattle inflatable, its raw material include betel nut, Cortex Magnoliae Officinalis, rhizoma atractylodis, radix glycyrrhizae, party
Ginseng, rhizoma zingiberis, Rhizoma Atractylodis Macrocephalae, malt, Fructus Aurantii, aspergillus oryzae culture dried object, calf Bacillus acidi lactici culture dried object.
Wherein, the parts by weight of each raw material are respectively betel nut 10-15 parts by weight, Cortex Magnoliae Officinalis 25-30 parts by weight, rhizoma atractylodis 15-
20 parts by weight, radix glycyrrhizae 5-10 parts by weight, Radix Codonopsis 20-25 parts by weight, rhizoma zingiberis 20-30 parts by weight, Rhizoma Atractylodis Macrocephalae 30-35 parts by weight, malt
10-15 parts by weight, Fructus Aurantii 30-35 parts by weight, aspergillus oryzae culture dried object 15-20 parts by weight, calf Bacillus acidi lactici culture drying
Thing 25-35 parts by weight.
Preferably, the parts by weight of each raw material are respectively 14 parts by weight of betel nut, 27 parts by weight of Cortex Magnoliae Officinalis, 16 weight of rhizoma atractylodis
Part, 8 parts by weight of radix glycyrrhizae, 22 parts by weight of Radix Codonopsis, 29 parts by weight of rhizoma zingiberis, 32 parts by weight of Rhizoma Atractylodis Macrocephalae, 12 parts by weight of malt, 34 weight of Fructus Aurantii
Part, 18 parts by weight of aspergillus oryzae culture dried object, 28 parts by weight of calf Bacillus acidi lactici culture dried object.
Wherein, the preparation method of the aspergillus oryzae culture dried object is as follows：
Aspergillus oryzae strain is activated using PDA culture medium, then expands culture aspergillus oryzae, is collected by centrifugation after thalline and bacterium is made is hanged
Liquid, dispenses to serum bottle, and aspergillus oryzae culture dried object is obtained by the way of vacuum freeze drying, and low temperature is protected after nitrogen-filled seal
Hide, the used time takes.
Wherein, the preparation method of the calf Bacillus acidi lactici culture dried object is as follows：
Calf Lactobacillus species are activated using MRS culture mediums, then expand culture calf Bacillus acidi lactici, thalline is collected by centrifugation
After bacteria suspension is made, mannitol is added in bacteria suspension, then dispense to serum bottle, is obtained by the way of vacuum freeze drying small
Cow's milk acidfast bacilli culture dried object, low-temperature preservation after nitrogen-filled seal, used time take.
Present invention also offers the preparation method of above-mentioned Chinese medicine microorganism formulation：
The betel nut, Cortex Magnoliae Officinalis, rhizoma atractylodis, radix glycyrrhizae, Radix Codonopsis, rhizoma zingiberis, Rhizoma Atractylodis Macrocephalae, malt, Fructus Aurantii are mixed by weight, shred big gun
System, the mixture after processing is placed in porcelain, pours into white wine to being totally submerged mixture, closed, stirring 1 in every 2 days first 10 days
It is secondary, stir weekly once within latter 35 days, dipping takes out press residue after supernatant after 45 days, press juice is mixed with supernatant, dense
Contracting drying for standby；Aspergillus oryzae culture dried object and the calf Bacillus acidi lactici culture drying of corresponding parts by weight are added before feeding
Thing, up to Chinese medicine microorganism formulation.
Below using embodiment come the embodiment that the present invention will be described in detail.
Embodiment 1：The formula of Chinese medicine microorganism formulation 1
14 parts by weight of betel nut, 27 parts by weight of Cortex Magnoliae Officinalis, 16 parts by weight of rhizoma atractylodis, 8 parts by weight of radix glycyrrhizae, 22 parts by weight of Radix Codonopsis, rhizoma zingiberis
29 parts by weight, 32 parts by weight of Rhizoma Atractylodis Macrocephalae, 12 parts by weight of malt, 34 parts by weight of Fructus Aurantii, 18 parts by weight of aspergillus oryzae culture dried object, calf
28 parts by weight of Bacillus acidi lactici culture dried object.
Embodiment 2：The formula of Chinese medicine microorganism formulation 2
10 parts by weight of betel nut, 25 parts by weight of Cortex Magnoliae Officinalis, 19 parts by weight of rhizoma atractylodis, 9 parts by weight of radix glycyrrhizae, 21 parts by weight of Radix Codonopsis, rhizoma zingiberis
23 parts by weight, 33 parts by weight of Rhizoma Atractylodis Macrocephalae, 11 parts by weight of malt, 32 parts by weight of Fructus Aurantii, 19 parts by weight of aspergillus oryzae culture dried object, calf
26 parts by weight of Bacillus acidi lactici culture dried object.
Embodiment 3：The formula of Chinese medicine microorganism formulation 3
12 parts by weight of betel nut, 29 parts by weight of Cortex Magnoliae Officinalis, 15 parts by weight of rhizoma atractylodis, 5 parts by weight of radix glycyrrhizae, 24 parts by weight of Radix Codonopsis, rhizoma zingiberis
25 parts by weight, 34 parts by weight of Rhizoma Atractylodis Macrocephalae, 14 parts by weight of malt, 30 parts by weight of Fructus Aurantii, 15 parts by weight of aspergillus oryzae culture dried object, calf
33 parts by weight of Bacillus acidi lactici culture dried object.
Embodiment 4：To the safety evaluatio of the Chinese medicine microorganism formulation
The experiment mice 96 that weight is 20 ± 2g is chosen, is randomly divided into four groups, every group 24, half male and half female；A group mouse
Gavage the Chinese herb feed additive 1 of the preparation of embodiment 1, concentration 10%；Chinese herbal medicine addition prepared by B group mouse gavagings embodiment 2
Agent 2, concentration 10%；Chinese herb feed additive 3 prepared by C group mouse gavagings embodiment 3, concentration 10%；D groups mouse gavaging steams
Distilled water；Dosage is daily 0.8ml/, continuous 30 days with confluent.
The general health of observation each group mouse daily, including situations such as diet, drinking-water, activity, weight；Respectively 5
My god, 15 days, after administration in 30 days 20 it is small when, every group is randomly selected the male mouse of 3 female mices and 3, carries out hematological examination, blood biochemical
Inspection and dissect；Remaining mouse was subjected to hematological examination, blood biochemistry checking and dissect in 10 days after drug withdrawal；Wherein, hematology
Inspection includes content of hemoglobin, red blood cell number, leukocyte count, platelet count and reticulocyte counts, blood biochemistry checking bag
Include glutamic-pyruvic transaminase, alkaline phosphatase, blood urea nitrogen, glutamic-oxalacetic transaminease, Human Serum Albumin and globulin, dissect master
If carry out liver,spleen,kidney, the histopathological examination of gastrointestinal organ.
Detected by observation, the diet of mouse, drinking-water, activity, weight are showed no exception in 40 days, also dead without finding
Case is died, the index of hematological examination, blood biochemistry checking and dissect is in normal range (NR), and the index of A-D groups is not aobvious
Write difference.This shows that Chinese herb feed additive provided by the invention is safe and non-toxic.
Embodiment 5：Contrast experiment
Packet：400 suitable nan yang yellow cattles of 2 one full year of life, physical condition are randomly divided into tetra- groups, every group 100 of A, B, C, D
Head.This 400 nan yang yellow cattles are raised under same environment, 3 DEG C of temperature, humidity 85%, feeds ice-cold aerogenesis feed, such as
Root tuber feed, ensilage etc.；Raise 100 days altogether.
Wherein, for there is the nan yang yellow cattle of inflatable symptom, A groups feed the Chinese medicine microorganism formulation 1 in embodiment 1, B groups
The Chinese medicine microorganism formulation 2 in embodiment 2 is fed, C groups feed the Chinese medicine microorganism formulation 3 in embodiment 3；D groups use background
Sodium thiosulfate therapy described in technology, dosage for 0.4 gram/suffer from ox per kg body weight, fed with warm water dissolving.Each group connects
Continuous medication 5 days, 1 time a day.Occur the nan yang yellow cattle of inflatable symptom in each group, subsequent experimental is not involved in after healing.
Index is as follows：
Inflatable refers to the increase of nan yang yellow cattle abdominal circumference, and the expansion of the part between the ribs and the hips portion is obvious, and touching is flexible, and percussion has tympanitic note, is short of breath,
No appetite and ruminate stopping；Recovery from illness refers to appetite and recovers normal, and pulse is steady, and walking is freely；Effective in cure finger pulp, which is enclosed, to be obviously reduced, the part between the ribs and the hips
Portion substantially softens, and breathing and pulse tend to be normal；Inefficacy, which refers to, maintains inflatable symptom.
Therapeutic effect is shown in Table 1.
Table 1：The therapeutic effect of each group
| Group | Inflatable/head | Recovery from illness/head | Effective in cure/head | Inefficacy/head | Cure rate (%) | Efficient (%) |
| A | 58 | 52 | 5 | 1 | 89.7 | 98.3 |
| B | 59 | 49 | 6 | 4 | 83.1 | 93.2 |
| C | 62 | 53 | 3 | 6 | 85.5 | 90.3 |
| D | 55 | 49 | 4 | 2 | 89.1 | 96.4 |
As it can be seen from table 1 using Chinese medicine microorganism formulation provided by the invention to there is the nan yang yellow cattle of inflatable symptom
Treated, can reach more than 90% effective percentage, illustrate the Chinese medicine microorganism formulation in terms of nan yang yellow cattle inflatable is treated
Significant effect；In addition, curative effect is best when the Chinese medicine microorganism formulation uses the formula in embodiment 1, cure rate can with effective percentage
Maintain an equal level with sodium thiosulfate therapy.
Embodiment of the present invention is not restricted to listed utilization in embodiment, and it is suitable for the various suitable present invention
Field, for those skilled in the art, easily realize other modification.Therefore without departing substantially from claim and homotype is being waited
Enclose under limited universal, the present invention is not limited to specific details and example described herein.
Claims (6)
1. a kind of Chinese medicine microorganism formulation for treating nan yang yellow cattle inflatable, it is characterised in that its raw material includes betel nut, Cortex Magnoliae Officinalis, grey
Art, radix glycyrrhizae, Radix Codonopsis, rhizoma zingiberis, Rhizoma Atractylodis Macrocephalae, malt, Fructus Aurantii, aspergillus oryzae culture dried object, calf Bacillus acidi lactici culture dried object.
2. the Chinese medicine microorganism formulation for the treatment of nan yang yellow cattle inflatable as claimed in claim 1, it is characterised in that each raw material
Parts by weight be respectively the betel nut 10-15 parts by weight, Cortex Magnoliae Officinalis 25-30 parts by weight, rhizoma atractylodis 15-20 parts by weight, radix glycyrrhizae 5-10 weight
Measure part, Radix Codonopsis 20-25 parts by weight, rhizoma zingiberis 20-30 parts by weight, Rhizoma Atractylodis Macrocephalae 30-35 parts by weight, malt 10-15 parts by weight, Fructus Aurantii 30-
35 parts by weight, aspergillus oryzae culture dried object 15-20 parts by weight, calf Bacillus acidi lactici culture dried object 25-35 parts by weight.
3. the Chinese medicine microorganism formulation for the treatment of nan yang yellow cattle inflatable as claimed in claim 2, it is characterised in that each raw material
Parts by weight be respectively 14 parts by weight of betel nut, 27 parts by weight of Cortex Magnoliae Officinalis, 16 parts by weight of rhizoma atractylodis, 8 parts by weight of radix glycyrrhizae, 22 weight of Radix Codonopsis
Measure part, 29 parts by weight of rhizoma zingiberis, 32 parts by weight of Rhizoma Atractylodis Macrocephalae, 12 parts by weight of malt, 34 parts by weight of Fructus Aurantii, 18 weight of aspergillus oryzae culture dried object
Measure part, 28 parts by weight of calf Bacillus acidi lactici culture dried object.
4. the preparation method of the Chinese medicine microorganism formulation for the treatment of nan yang yellow cattle inflatable, its feature exist as claimed in claim 2 or claim 3
In, by the betel nut, the Cortex Magnoliae Officinalis, the rhizoma atractylodis, the radix glycyrrhizae, the Radix Codonopsis, the rhizoma zingiberis, the Rhizoma Atractylodis Macrocephalae, the malt,
The Fructus Aurantii mixes by weight, and chopping processes, and the mixture after processing is placed in porcelain, it is mixed to being totally submerged to pour into white wine
Compound, it is closed, stir 1 time within every 2 days first 10 days, stir weekly once within latter 35 days, dipping takes out supernatant after 45 days, and squeezing is residual
Slag obtains press juice, and the press juice is mixed with the supernatant, is concentrated and dried spare；Phase is added before feeding nan yang yellow cattle
The aspergillus oryzae culture dried object of parts by weight and the calf Bacillus acidi lactici culture dried object are answered, up to the Chinese medicine microorganism
Preparation.
5. the preparation method of the Chinese medicine microorganism formulation for the treatment of nan yang yellow cattle inflatable as claimed in claim 4, it is characterised in that
The preparation method of the aspergillus oryzae culture dried object is as follows：Aspergillus oryzae strain is activated using PDA culture medium, then expands culture meter Qu
It is mould, bacteria suspension is made after thalline is collected by centrifugation, dispenses to serum bottle, the meter Qu is obtained by the way of vacuum freeze drying
Mould culture dried object, low-temperature preservation after nitrogen-filled seal, used time take.
6. the preparation method of the Chinese medicine microorganism formulation for the treatment of nan yang yellow cattle inflatable as claimed in claim 4, it is characterised in that
The preparation method of the calf Bacillus acidi lactici culture dried object is as follows：Calf Lactobacillus species are activated using MRS culture mediums, then
Expand culture calf Bacillus acidi lactici, bacteria suspension is made after thalline is collected by centrifugation, mannitol is added in bacteria suspension, then dispenses to blood
Clear bottle, the calf Bacillus acidi lactici culture dried object is obtained by the way of vacuum freeze drying, and low temperature is protected after nitrogen-filled seal
Hide, the used time takes.
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Citations (3)
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|---|---|---|---|---|
| CN103642695A (en) * | 2013-08-28 | 2014-03-19 | 浙江工业大学 | Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation |
| CN104491043A (en) * | 2014-11-06 | 2015-04-08 | 宣威华安农牧科技开发有限公司 | Chinese herbal medicine treating cattle flatulence |
| CN107279468A (en) * | 2017-07-01 | 2017-10-24 | 曲靖双胞胎饲料有限公司 | A kind of probiotics for preventing and treating grice diarrhoea |
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2017
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| CN103642695A (en) * | 2013-08-28 | 2014-03-19 | 浙江工业大学 | Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation |
| CN104491043A (en) * | 2014-11-06 | 2015-04-08 | 宣威华安农牧科技开发有限公司 | Chinese herbal medicine treating cattle flatulence |
| CN107279468A (en) * | 2017-07-01 | 2017-10-24 | 曲靖双胞胎饲料有限公司 | A kind of probiotics for preventing and treating grice diarrhoea |
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Application publication date: 20180420 |