CN107873360A

CN107873360A - A kind of interplanting method of purple napier grass and mulberry tree

Info

Publication number: CN107873360A
Application number: CN201711134707.1A
Authority: CN
Inventors: 易显凤; 赖志强; 姚娜; 韦锦益; 丘金花; 陈冬冬; 史静; 廖晓光
Original assignee: Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
Current assignee: Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
Priority date: 2017-11-16
Filing date: 2017-11-16
Publication date: 2018-04-06
Anticipated expiration: 2037-11-16
Also published as: CN107873360B

Abstract

The present invention relates to planting technology field, and in particular to a kind of interplanting method of purple napier grass and mulberry tree, specifically includes the processing of step (1) purple napier grass seedling stem：Ripe purple napier grass seedling stem is chopped to one section of two section, select one section of two bud seedling stem, then seedling stem is soaked using antimicrobial fluid and takes out within 0.5 1.5 minutes nature and dry in the air and put, the antimicrobial fluid is mixed by following raw material according to parts by weight, 15 parts of Fructus Corni extract solution, 38 parts of Coreopsis tinctoria extract solution, 35 parts of bur beggar-ticks extract solution, 80 100g/L brown sugar, 10 15g/L compound sodium nitrophenolates；(2) site preparation；(3) interplant；(5) field management；(4) gather in.Advantage：Found by research during purple napier grass is planted, the well developed root system of napier grass, and root system has the ability for adsorbing some heavy metals, reduce the content of beary metal in soil, adsorption capacity is improved after being applied with the base manure of independent research, the content of beary metal in mulberry leaf is greatly reduced, the spinning amount of silkworm is improved when carrying out feeding silkworm, there is good economic benefit.

Description

A kind of interplanting method of purple napier grass and mulberry tree

【Technical field】

The present invention relates to planting technology field, and in particular to a kind of interplanting method of purple napier grass and mulberry tree.

【Background technology】

Mulberry tree (Morus alba L.) belongs to Moraceae (Moraceae) Morus (Morus), is a kind of dioecian plant, It is widely used in sericulture feed etc..For the survival rate of silkworm not just for the environment of cultivation, emphasis further comprises the mulberry leaf product of feeding Matter closely affects the survival rate of silkworm, vitality etc..And with the aggravation of mankind's activity, the heavy metal that is discharged in environment Polluter is increasing, causes how much the content of beary metal of local area improves, because finding one kind during mulberry tree is planted The implantation methods that content of beary metal in mulberry leaf can be reduced are extremely urgent.

Purple napier grass (Pennisetum purpureumSchumab cv.Purple) originates in Brazil, has plant high Greatly, well developed root system, cane and blade are in purple, vegetative propagation, cold-resistant, strong stress resistance tiller is more, yield is high, purple napier grass root System is flourishing, and sealing ground is fast, and coverage strength is big, and fixing of soil and conservation of water ability is strong.Therefore, purple napier grass is that a kind of feeding and ecology is simultaneous Standby good grass seed, is worthy of popularization.

【The content of the invention】

The goal of the invention of the present invention is：It is an object of the invention to provide the interplanting method of a kind of purple napier grass and mulberry tree, Found by research during purple napier grass is planted, the well developed root system of napier grass, and root system has the energy for adsorbing some heavy metals Power, the content of beary metal in soil is reduced, improve adsorption capacity after being applied with the base manure of independent research, greatly reduce Content of beary metal in mulberry leaf, the spinning amount of silkworm is improved when carrying out feeding silkworm, there is good economic benefit.

To achieve these goals, the technical solution adopted by the present invention is as follows：

A kind of interplanting method of purple napier grass and mulberry tree, specifically includes following steps：

(1) purple napier grass seedling stem is handled：Ripe purple napier grass seedling stem is chopped to one section of a section, selects a two bud seedling stems of section, Then seedling stem is taken out nature and dried in the air using antimicrobial fluid immersion 0.5-1.5 minutes and put,

The antimicrobial fluid is mixed by following raw material according to parts by weight, Fructus Corni extract solution 1-5 parts, Coreopsis tinctoria extraction Liquid 3-8 parts, bur beggar-ticks extract solution 3-5 parts, 80-100g/L brown sugar, 10-15g/L compound sodium nitrophenolates；

(2) site preparation：Cave is opened in the fertile field of selection soil property, and the spacing in the rows per cave is 200-300 centimetres of 20-30 cm x, often Apply base manure before bunch planting kind, per 0.1 kilogram of cave, then purple napier grass seedling stem is played and put with ditch, digging purple napier grass kind Stem covers, and it is 60% to water to water content, and every plant of spacing of purple napier grass seedling stem of often row sowing is 30-40 centimetres, is sowed 10 days The growing way of seedling stem seedling is observed afterwards, carries out processing of filling the gaps with seedlings；

The base manure is prepared by following raw material：N P and K 15:15:15 composite fertilizer, E.cancerogenus intestines bars Bacterium, P. amygdali pseudomonads, P.corrugata pseudomonads, glucose are 100 according to weight ratio:0.01-0.05: 0.02-0.03:0.01-0.02:0.1-0.3 is used after being mixed；

(3) interplant：By growing way stalwartness, high 15-18 centimetres of mulberry of well developed root system while carrying out purple napier grass and filling the gaps with seedlings Sapling is interplanted on the position among every row purple napier grass, and the spacing in the rows of mulberry saplings is 200-300 centimetres of 80-120 cm x；

(4) field management：

A, foliar fertilizer is sprayed to purple napier grass after high 20-30 centimetres of the seedling that purple napier grass seedling stem is grown, dose is 100-150 kgs/acre；

B, weeding processing is carried out to planting site herbicide spraying, the amount of spraying is up to after 40-50 centimetres after purple napier grass 50-60 kgs/acre；

C, 2-3 days after weeding processing, it is that the center of circle digs wide 10 lis of 5 centimetres of depths with 20 centimetres apart from purple napier grass stem The fertilising circle of rice, then applies top dressing, and every dose is 0.3-0.6 kilograms；

After mulberry tree length it is paramount after 30 centimetres with trunk 20-30 centimetres be that the center of circle digs wide 20 centimetres of depths, 20 centimetres of fertilising circles, Then top dressing is applied, every dose is 0.5-0.8 kilograms；

D, pest repellant was sprayed to mulberry tree, purple napier grass at interval of 10 days, the amount of spraying is 50-60 kgs/acre；

(5) gather in：Gathered in after purple napier grass is grown to 100-110 centimetres, field is repeated after purple napier grass again long new talent Between the step of managing.

Further illustrate, the foliar fertilizer is prepared by following methods：By mulberry tree dead leaf carry out fermentation rot after It is soaked in water 1-2 days, filters to take to obtain nutrient solution, in parts by weight by nutrient solution 30-50 parts, ammonium sulfate 5-10 parts, urine Plain 3-8 parts, potassium sulfate 3-8 parts, potassium dihydrogen phosphate 3-8 parts, aliphatic acid polyethenoxy base ester 0.1-0.5 parts, water 30-40 parts, drawing Open powder 0.1-1 parts, polycarboxylate 0.1-1 parts, xanthans 0.1-2 parts mixing can obtain foliar fertilizer.

Further illustrate, the top dressing is prepared by following methods：According to percetage by weight, 10%- is weighed The 15% corn dregs of rice, 10%-15% soya beans shell, 10%-15% phosphorus mine tailing, 15%-20% bagasse, 15%-20% lotus straw Stalk, 5%-10% vanillas Aqueous extracts, 5%-10% strawberries Aqueous extracts, 5%-10% bacillus subtilises, 2%-8% plants breast Fermented after bacillus, the mixing of 3%-8% cellulomonas, the temperature for controlling fermentation is 35 ± 2 DEG C, and ferment at constant temperature is after 5-10 days It can obtain top dressing.

Further illustrate, the pest repellant is prepared by following raw material in parts by weight：Galium verum 5-10 Part, argy wormwood 3-5 parts, peppermint 3-8 parts, aloe 5-8 parts, capsicum 5-8 parts.

Further illustrate, Galium verum is to add fresh Galium verum after 2 times of water to carry out refluxing extraction 2 times of addition after 2 hours The ethanol solution that the mass fraction of volume is 75% water-bath at 38 DEG C is extracted 30 minutes, and filtrate is collected by filtration, obtains Galium verum Extract solution.

Further illustrate, argy wormwood, which adds 100 grams of argy wormwood herb in 300 grams of water, to carry out heating infusion 30 minutes, mistake Leaching filtrate, obtains felon herb raffinate.

Further illustrate, peppermint smashs fresh mint leaf to pieces, add ethanol solution that 2 times of volume mass fractions are 75% in Refluxing extraction 30 minutes at 30 DEG C, extract 3 times altogether, merge the filtrate of 3 filterings.

Further illustrate, aloe smashs fresh aloe to pieces, add ethanol solution that 2 times of volume mass fractions are 75% in Refluxing extraction 30 minutes at 30 DEG C, extract 3 times altogether, merge the filtrate of 3 filterings, obtain extract solution from aloe.

Further illustrate, after capsicum crushes chilli, using adding, 0.5 times of water immersion 1-2 hour is aqueous to chilli Rate reaches more than 60%, be subsequently added into 2 times of volume mass fractions be 75% ethanol solution at 30 DEG C refluxing extraction 30 divide Clock, extract 3 times altogether, merge the filtrate of 3 filterings.

Further illustrate, the application method of pest repellant is in parts by weight by Galium verum extract solution 5-10 parts, argy wormwood 10-20 part water is converted after extract solution 3-5 parts, peppermint extract solution 3-8 parts, extract solution from aloe 5-8 parts, the mixing of capsicum extract 5-8 parts After use.

Further instruction, the Fructus Corni extract solution are to dry Fructus Corni bark after rear ultramicrowave crushes to add, and are added The ethanol solution that the mass concentration for entering 2-3 times of volume is 70%-80% carries out alcohol extracting 3-5 minutes, and it is auxiliary that microwave is carried out during alcohol extracting Alcohol extracting is helped, the power of microwave is 350-400W, and the first filtrate is taken after being filtered after alcohol extracting, takes the filtrate of 100-200 milliliters first to add 1-3 grams of amylase, carry out Soxhlet refluxing extraction and filter to take the second filtrate 3 times, each 35 DEG C of constant temperature extracts 10 minutes, by filter 23 The second secondary filtrate, which merges, can obtain Fructus Corni extract solution.

Further instruction, the Coreopsis tinctoria extract solution are the water that 3-5 times of volume is added after fresh Coreopsis tinctoria flower is smashed to pieces Carry out carrying out rotating centrifugal separation after decocting 20-30 minutes, centrifuge RPMs 2000r/min, centrifuge 2-5 minutes, isolate solid Body material, filtrate is gone, carry out 2-3 decoction, rotating centrifugal separation, the filtrate of 3 times is merged, then by the filtrate after merging Three layers of filtered through gauze are carried out, are repeated 2-3 times, merging filtrate can obtain Coreopsis tinctoria extract solution.

Further instruction, the bur beggar-ticks extract solution are to crushed 10 mesh sieves after bur beggar-ticks herb is dried, and are added Enter after 3-5 times of water carries out 32-35 DEG C of water-bath refluxing extraction 1-2 hour and filter to take filtrate, refluxing extraction 2-3 times, merge bur beggar-ticks Filtrate, filter residue is added into pectase, cellulase, water and carries out adverse current extraction, Extracting temperature is 32-35 DEG C, and extraction time is 30-50 minutes, bur beggar-ticks filter residue liquid is obtained, bur beggar-ticks filtrate and bur beggar-ticks filter residue liquid are mixed and can obtain bur beggar-ticks extraction Liquid.

Further illustrate, the E.cancerogenus enterobacterias are 0.37 × 10 effectively containing bacterium number⁴-0.52×10⁴ Individual/mL, P.amygdali pseudomonad is 0.18 × 10 effectively containing bacterium number⁴-0.25×10⁵Individual/mL, P.corrugata is false Monad is 0.24 × 10 effectively containing bacterium number⁴-0.32×10⁴Individual/mL.

Further illustrate, the E.cancerogenus enterobacterias, P.amygdali pseudomonads, P.corrugata are false Monad also passes through activation process；

The activation condition of E.cancerogenus enterobacterias is to take 1mL E.cancerogenus enterobacterias to be inoculated in be equipped with 32 DEG C of constant-temperature table culture 18-20 hours of 15mL activation mediums；The activation culture based formulas is activation medium tryptose Peptone 5-10g/L, extract of soybean 3-5g/L, ammonium sulfate 1-3g/L, potassium dihydrogen phosphate 0.1-0.3g/L, pH 7.4 ± 0.2.

It is respectively to take 1mL to take 1mL P.amygdali pseudomonads, the activation condition of P.corrugata pseudomonads P. amygdali pseudomonads, P.corrugata pseudomonads are inoculated in equipped with 35 DEG C of constant temperature in 15mL activation mediums respectively Shaking table culture 24-25 hours；The activation culture based formulas is gelatin peptone 10.0-12.0g, tryptone 5.0-8.0g, soybean Extract 3-8g, ammonium sulfate 1-3g, potassium dihydrogen phosphate 0.2g, potassium sulfate 10.0g, magnesium chloride 1.4g, agar 15.0g, pH 7.1 ±0.2。

In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：

(1) pest repellant of the application is free of chemical substance, is all middle medicinal substances, non-toxic and safe, is fed after harvesting purple napier grass The nontoxic residual of animal is eaten, securely and reliably.It is total well known, various pests are had during plant growth, has outer infringement worm, there is environment The insect of growth, there is insect that strain is evolved into etc., general prior art is to spray a type of agriculture for a class pest Medicine, noxious material residue excess on the frequent purple napier grass of pesticide application is caused, causes to need to carry out disinfection before feeding animals Cleaning ensures that the green non-poisonous purple napier grass of animal edible ensures the production quality of animal.Studied through the applicant and find that peppermint carries Take liquid to contain menthol, felon herb raffinate, Galium verum extract solution and contain volatile oil, the effect with expelling parasite, but natural materials When being not added with chemical substance fusion, the time existing for menthol, volatile oil is of short duration, and anthelminthic effect is not lasting, and addition aloe carries Take liquid, capsicum extract to contain aloin and capsaicine respectively, the structure of volatile oil, alcohol can be consolidated so that their structure Firm slow release, two extracts serve booster action containing volatile oil, terpene substances, and Galium verum extract solution is also Also largely contain vulgarin containing iridoid, organic acid, anthraquinone, felon herb raffinate, extract solution from aloe contains aloe soap Careless glycosides, aloe Chrysophanol, capsicum extract capsaicine can expand the scope of expelling parasite, suppress the growth brown spot of pernicious bacteria Powdery mildew, rust, sclerotiniose, mulberry wasting disease, mulberry mosaic disease, mulberry epidemic disease, the small spodogram of mulberry are first-class, and the taste containing uniqueness can drive The insects such as mosquito, Liriomyza, aphid, smelly wind worm, armyworm, chafer, noctuid, euproctis similis, snail, aphid, tetranychid, thrips are caught up with, And that poisons works well, and ensure that the healthy growth of purple napier grass, while the quality of mulberry leaf after growing of mulberry is also improved, So that the probability that silkworm disease occurs when breeding silkworms substantially reduces.

(2) found by research using 5%-10% bacillus subtilises, 2%-8% Lactobacillus plantarums, 3%-8% fibers Bacillus decomposes the corn dregs of rice, soya bean shell, phosphorus mine tailing, bagasse, lotus stalk, discharge substantial amounts of carbon, nitrogen, potassium element, The organic matters such as P elements, inorganic matter, content of organics 26-31%, inorganic matter 28-35%, contain rapid available phosphorus 3.86-4.21mg/ Kg, amino acid reach more than 25%；And what P elements easily contained in itself with soil causes P elements inactivation precipitation, in order that Obtaining P elements itself band matcoveredn prevents easy in inactivation, and addition 5%-10% vanillas Aqueous extracts, 5%-10% are found by research Strawberry Aqueous extracts, both mutually promote the activity of firm P elements, play a protective role, the latter contains tartaric acid, oxalic acid, third Ketone acid, anthocyanidin, tartaric acid, oxalic acid, pyruvic acid, can scavenging hydroxyl ability, there is preferable oxidation resistance, it is preceding Person contains the activity that substantial amounts of oxalic acid, anthocyanidin, tartaric acid can strengthen the phosphonium ion of firm activation, strengthens oxidation resistance, and two The combination of kind extract can reach mutual assistance, the effect of dual guarantee, reach by the activity for detecting phosphatase in soil 48 0.0008mg/g-0.0010mg/g after hour so that purple napier grass can be in the sunlight of half sunshade under mulberry tree in interplanting after top dressing The lower growth that sunlight can be made full use of to carry out photosynthesis promotion purple napier grass, can be in growth period multi-harvest.

(3) use of the antimicrobial fluid of the application is that the plantation for being directed to purple napier grass in the prior art is generally seedling stem sowing, Typically occur that the phenomenon that needs are filled the gaps with seedlings reaches every mu more than 50 plants, study and found due to purple napier grass kind by the applicant Stem sweetness is easily gnawed by mouse, can typically prevent rat poison, but safety problem be present, also the reason for be that seedling stem can not The purple napier grass bud not long or underfed phenomenon of seedling, Fructus Corni extract solution, Coreopsis tinctoria extraction are caused to antimycotic growth The ability that liquid, bur beggar-ticks extract solution are respectively provided with antibacterial suppresses the formation of harmful population, but does not influence beneficial microorganism life It is long, and not only the ability with anti-pernicious bacteria has and overlapping also has difference three's extract solution so that the scope of antibacterial expands, and increases Strong antibacterial ability, find that using the proportioning of the application best antibacterial effect and the effect to supplement the nutrients can be reached by research, The effect of extra-nutrition is to contain large amount of organic matter in immersion process in three kinds of extract solutions to absorb, but assimilation effect It is bad, and the power element deficiency of the promotion kind Stem nematode contained, brown sugar can promote three as subsidy power element, compound sodium nitrophenolate The absorption of kind of extract solution and brown sugar, the energy for improving seedling stem itself storage improve emergence rate, and are interplanted in growing of mulberry after 1 year Purple napier grass, the organic substance that purple napier grass is decomposed in soil are easily robbed the Nutrient Absorption of purple napier grass by mulberry tree, purple The nutrient rate of being absorbed and used that color napier grass itself is decomposed reduces.

(4) plantation of mulberry tree is bred silkworms using mulberry leaf, and the constitution situation of silkworm and the mulberry leaf of feeding have important association, And after silkworm is eaten containing the too high mulberry leaf of content of beary metal, accumulation digestion serious wound can will not be caused to silkworm in vivo Evil, the vitality of silkworm is influenceed, the silkworm death rate is big when causing spinning evening, the reduction of spinning amount, intake content of beary metal too high It is big to improve.Therefore the applicant by research find using E.cancerogenus enterobacterias, P.amygdali pseudomonads, Cooperated after the combination of P.corrugata pseudomonads with excellent absorption, decompose the ability of heavy metal, and find activation Compound bacteria absorption afterwards, capacity of decomposition are stronger, greatly reduce the ability that plant absorption utilizes heavy metal.

【Embodiment】

In order to facilitate the understanding of the purposes, features and advantages of the present invention, with reference to specific embodiment pair The embodiment of the present invention is described in detail.Elaborate many details in order to abundant in the following description Understand the present invention.But the invention can be embodied in many other ways as described herein, those skilled in the art Similar improvement can be done in the case of without prejudice to intension of the present invention, therefore the present invention is not by following public specific implementation Limitation.

Embodiment 1：

(1) purple napier grass seedling stem is handled：Ripe purple napier grass seedling stem is chopped to one section of two section, selects one section of two bud seedling stem, Then seedling stem is dried in the air using 0.5 minute taking-up nature of antimicrobial fluid immersion and put,

The antimicrobial fluid is mixed by following raw material according to parts by weight, 1 part of Fructus Corni extract solution, Coreopsis tinctoria extract solution 3 parts, 3 parts of bur beggar-ticks extract solution, 80g/L brown sugar, 10g/L compound sodium nitrophenolates；

Wherein Fructus Corni extract solution is to dry Fructus Corni bark after rear ultramicrowave crushes to add, and adds the matter of 2 times of volumes Measure the ethanol solution that concentration is 70% and carry out alcohol extracting 3 minutes, microwave radiation technology alcohol extracting is carried out during alcohol extracting, the power of microwave is 350W, the first filtrate is taken after being filtered after alcohol extracting, take 100 milliliter of first filtrate to add 1 gram of amylase, carry out Soxhlet refluxing extraction 3 Secondary to filter to take the second filtrate, each 35 DEG C of constant temperature extracts 10 minutes, the second filtrate of filter 23 time is merged and can obtain mountain Zhu Cornel extract solution；

Wherein Coreopsis tinctoria extract solution is that the water that 3 times of volumes are added after fresh Coreopsis tinctoria flower is smashed to pieces is carried out after decocting 20 minutes Rotating centrifugal separation is carried out, centrifuge RPMs 2000r/min, centrifuges 2 minutes, isolates solid matter, goes filtrate, is carried out 2 times Decoct, rotating centrifugal separation, the filtrate of 3 times is merged, the filtrate after merging is then subjected to three layers of filtered through gauze, repeats 2 Secondary, merging filtrate can obtain Coreopsis tinctoria extract solution；

Wherein bur beggar-ticks extract solution is that 10 mesh sieves are crushed after bur beggar-ticks herb is dried, and adds 3 times of water and carries out 32 DEG C water-bath refluxing extraction filters to take filtrate after 1 hour, refluxing extraction 2 times, merge bur beggar-ticks filtrate, by filter residue add pectase, Cellulase, water carry out adverse current extraction, and Extracting temperature is 32 DEG C, and extraction time is 30 minutes, obtains bur beggar-ticks filter residue liquid, will Bur beggar-ticks filtrate and the mixing of bur beggar-ticks filter residue liquid can obtain bur beggar-ticks extract solution；

(2) site preparation：Cave is opened in the fertile field of selection soil property, and the spacing in the rows per cave is 200 centimetres of 20 cm x, before bunch planting kind Apply base manure, per 0.1 kilogram of cave, then purple napier grass seedling stem played and put with ditch, digging the covering of purple napier grass seedling stem, Watering to water content is 60%, and every plant of spacing of purple napier grass seedling stem of often row sowing is 30 centimetres, and sowing observes seedling stem after 10 days The growing way of seedling, carry out processing of filling the gaps with seedlings；

The base manure is prepared by following raw material：N P and K 15:15:15 composite fertilizer, E.cancerogenus intestines bars Bacterium, P. amygdali pseudomonads, P.corrugata pseudomonads, glucose are 100 according to weight ratio:0.01:0.02: 0.01:0.1 mixed after use；

Further illustrate, E.cancerogenus enterobacterias are 0.37 × 10 effectively containing bacterium number⁴Individual/mL, P.amygdali pseudomonads are 0.18 × 10 effectively containing bacterium number⁴Individual/mL, P.corrugata pseudomonad effectively contains bacterium Number is 0.24 × 10⁴Individual/mL；

The activation condition of E.cancerogenus enterobacterias is to take 1mL E.cancerogenus enterobacterias to be inoculated in be equipped with The constant-temperature table culture 18 hours of 32 DEG C of 15mL activation mediums；The activation culture based formulas is activation medium tryptone 5g/L, extract of soybean 3g/L, ammonium sulfate 1g/L, potassium dihydrogen phosphate 0.1g/L, pH 7.4 ± 0.2；

It is respectively to take 1mL to take 1mL P.amygdali pseudomonads, the activation condition of P.corrugata pseudomonads P. amygdali pseudomonads, P.corrugata pseudomonads are inoculated in equipped with 35 DEG C of constant temperature in 15mL activation mediums respectively Shaking table culture 24 hours；The activation culture based formulas is gelatin peptone 10.0g, tryptone 5.0g, extract of soybean 3g, sulphur Sour ammonium 1g, potassium dihydrogen phosphate 0.2g, potassium sulfate 10.0g, magnesium chloride 1.4g, agar 15.0g, pH 7.1 ± 0.2；

(3) interplant：By growing way stalwartness, high 15 centimetres of mulberry saplings of well developed root system while carrying out purple napier grass and filling the gaps with seedlings On the position among every row purple napier grass, the spacing in the rows of mulberry saplings is 200 centimetres of 80 cm x for interplanting；

(4) field management：

A, foliar fertilizer is sprayed to purple napier grass after high 20-30 centimetres of the seedling that purple napier grass seedling stem is grown, dose is 100 kgs/acre；Foliar fertilizer is by mulberry tree dead leaf be soaked in water 1 day after fermentation is rotted, and filters to take to obtain nutrient solution, according to Parts by weight meter is by 30 parts of nutrient solution, 5 parts of ammonium sulfate, 3 parts of urea, 3 parts of potassium sulfate, 3 parts of potassium dihydrogen phosphate, aliphatic acid polyoxy 0.1 part of 0.1 part of vinyl esters, 30 parts of water, 0.1 part of pull open powder, 0.1 part of polycarboxylate, xanthans mixing can obtain；

B, after purple napier grass up to weeding processing after 40 centimetres, is carried out to planting site herbicide spraying, the amount of spraying is 50 Kg/acre；

C, 2 days after weeding processing, it is that the center of circle digs wide 10 centimetres of 5 centimetres of depths with 20 centimetres apart from purple napier grass stem Fertilising circle, then apply top dressing, every dose is 0.3 kilogram, after paramount 30 centimetres of mulberry tree length with trunk 20 centimetres be The center of circle digs 20 centimetres of fertilising circles of wide 20 centimetres of depths, then applies top dressing, and every dose is 0.5 kilogram, the top dressing according to Percetage by weight, weigh the 10% corn dregs of rice, 10% soya bean shell, 10% phosphorus mine tailing, 20% bagasse, 20% lotus stalk, 10% Vanilla Aqueous extracts, 10% strawberry Aqueous extracts, 5% bacillus subtilis, 2% Lactobacillus plantarum, the mixing of 3% cellulomonas are laggard Row fermentation, the temperature for controlling fermentation are 35 ± 2 DEG C, and ferment at constant temperature can obtain top dressing after 5-10 days；

D, pest repellant was sprayed to mulberry tree, purple napier grass at interval of 10 days, the amount of spraying is 50 kgs/acre；The pest repellant is Used after converting 10 parts of water after 5 parts of 5 parts of Galium verum, 3 parts of argy wormwood, 3 parts of peppermint, 5 parts of aloe, capsicum mixing；

Wherein Galium verum is that fresh Galium verum is added after 2 times of water to the matter for carrying out refluxing extraction 2 times of volumes of addition after 2 hours The ethanol solution that amount fraction is 75% water-bath at 38 DEG C is extracted 30 minutes, and filtrate is collected by filtration, obtains Galium verum extract solution；

Wherein argy wormwood, which adds 100 grams of argy wormwood herb in 300 grams of water, carries out heating infusion 30 minutes, filters to take filter Liquid, obtain felon herb raffinate；

Wherein peppermint smashs fresh mint leaf to pieces, and the ethanol solution that 2 times of volume mass fractions of addition are 75% is at 30 DEG C Refluxing extraction 30 minutes, extract 3 times altogether, merge the filtrate of 3 filterings；

Wherein aloe smashs fresh aloe to pieces, and the ethanol solution that 2 times of volume mass fractions of addition are 75% is at 30 DEG C Refluxing extraction 30 minutes, extract 3 times altogether, merge the filtrate of 3 filterings, obtain extract solution from aloe；

After wherein capsicum crushes chilli, reach 60% using 0.5 times of addition water immersion 1 hour to chilli moisture content More than, be subsequently added into 2 times of volume mass fractions be 75% ethanol solution at 30 DEG C refluxing extraction 30 minutes, extract 3 altogether It is secondary, merge the filtrate of 3 filterings；

Comparative example 1：

Same as Example 1, difference is that antiseptic is counted carried including 3 parts of Coreopsis tinctoria extract solution, bur beggar-ticks in parts by weight Take 3 parts of liquid, 80g/L brown sugar, 10g/L compound sodium nitrophenolates.

Comparative example 2：

Same as Example 1, difference is that antiseptic is counted carried including 1 part of Fructus Corni extract solution, bur beggar-ticks in parts by weight Take 3 parts of liquid, 80g/L brown sugar, 10g/L compound sodium nitrophenolates.

Comparative example 3：

Same as Example 1, it is red including 1 part of Fructus Corni extract solution, 80g/L that difference is that antiseptic is counted in parts by weight Sugar, 10g/L compound sodium nitrophenolates.

Comparative example 4：

Same as Example 1, it is red including 3 parts of bur beggar-ticks extract solution, 80g/L that difference is that antiseptic is counted in parts by weight Sugar, 10g/L compound sodium nitrophenolates.

Comparative example 5：

Same as Example 1, it is red including 3 parts of Coreopsis tinctoria extract solution, 80g/L that difference is that antiseptic is counted in parts by weight Sugar, 10g/L compound sodium nitrophenolates.

Comparative example 6：

Same as Example 1, difference is that antiseptic is counted including 3 parts of bur beggar-ticks extract solution, the multiple nitre of 10g/L in parts by weight Phenol sodium.

Comparative example 7：

Same as Example 1, difference is that antiseptic is counted carried including 1 part of Fructus Corni extract solution, Coreopsis tinctoria in parts by weight Take 3 parts of liquid, 3 parts of bur beggar-ticks extract solution, 80g/L brown sugar.

To embodiment 1, comparative example 1-5, blank control, each one mu of antimicrobial fluid immersion seedling stem plantation, soil environment phase are used Together, sow purple napier grass seedling stem after observe purple napier grass emergence rate, emergence it is equal when, growing way color situations such as such as table 1：

Table 1

As it can be seen from table 1 the emergence rate of purple napier grass can be improved using the antiseptic of the application, and contain three kinds of extractions The effect of liquid is more preferable than two kinds (comparative example 1-2) or a kind of (comparative example 3-5) antibacterial effect；From embodiment 1, comparative example 6- 7, the seedling-growing time that purple napier grass is improved simultaneously added with the antibacterial liquid energy of brown sugar and compound sodium nitrophenolate is seen in blank control, it was demonstrated that three Kind of extract, which has, to be provided nutritional ingredient and promotes seedling stem emergence, and demonstrate can grow after the growth of purple napier grass it is more Purple napier grass growing way must be caused more preferably by taking root.

Embodiment 2：

(1) purple napier grass seedling stem is handled：Ripe purple napier grass seedling stem is chopped to one section of two section, selects one section of two bud seedling stem, Then seedling stem is dried in the air using 1.5 minutes taking-up natures of antimicrobial fluid immersion and put,

The antimicrobial fluid is mixed by following raw material according to parts by weight, 5 parts of Fructus Corni extract solution, Coreopsis tinctoria extract solution 8 parts, 5 parts of bur beggar-ticks extract solution, 100g/L brown sugar, 15g/L compound sodium nitrophenolates；

Wherein Fructus Corni extract solution is to dry Fructus Corni bark after rear ultramicrowave crushes to add, and adds the matter of 3 times of volumes Measure the ethanol solution that concentration is 80% and carry out alcohol extracting 5 minutes, microwave radiation technology alcohol extracting is carried out during alcohol extracting, the power of microwave is 400W, the first filtrate is taken after being filtered after alcohol extracting, take 200 milliliter of first filtrate to add 3 grams of amylase, carry out Soxhlet refluxing extraction 3 Secondary to filter to take the second filtrate, each 35 DEG C of constant temperature extracts 10 minutes, the second filtrate of filter 23 time is merged and can obtain mountain Zhu Cornel extract solution；

Wherein Coreopsis tinctoria extract solution is that the water that 5 times of volumes are added after fresh Coreopsis tinctoria flower is smashed to pieces is carried out after decocting 30 minutes Rotating centrifugal separation is carried out, centrifuge RPMs 2000r/min, centrifuges 5 minutes, isolates solid matter, goes filtrate, is carried out 3 times Decoct, rotating centrifugal separation, the filtrate of 3 times is merged, the filtrate after merging is then subjected to three layers of filtered through gauze, repeats 3 Secondary, merging filtrate can obtain Coreopsis tinctoria extract solution；

Wherein bur beggar-ticks extract solution is that 10 mesh sieves are crushed after bur beggar-ticks herb is dried, and adds 5 times of water and carries out 35 DEG C water-bath refluxing extraction filters to take filtrate after 2 hours, refluxing extraction 3 times, merge bur beggar-ticks filtrate, by filter residue add pectase, Cellulase, water carry out adverse current extraction, and Extracting temperature is 35 DEG C, and extraction time is 50 minutes, obtains bur beggar-ticks filter residue liquid, will Bur beggar-ticks filtrate and the mixing of bur beggar-ticks filter residue liquid can obtain bur beggar-ticks extract solution；

(2) site preparation：Cave is opened in the fertile field of selection soil property, and the spacing in the rows per cave is 300 centimetres of 30 cm x, before bunch planting kind Apply base manure, per 0.1 kilogram of cave, then purple napier grass seedling stem played and put with ditch, digging the covering of purple napier grass seedling stem, Watering to water content is 60%, and every plant of spacing of purple napier grass seedling stem of often row sowing is 40 centimetres, and sowing observes seedling stem after 10 days The growing way of seedling, carry out processing of filling the gaps with seedlings；

The base manure is prepared by following raw material：N P and K 15:15:15 composite fertilizer, E.cancerogenus intestines bars Bacterium, P. amygdali pseudomonads, P.corrugata pseudomonads, glucose are 100 according to weight ratio:0.05:0.03: 0.02:0.3 mixed after use；

Further illustrate, the E.cancerogenus enterobacterias are 0.52 × 10 effectively containing bacterium number⁴Individual/mL, P.amygdali pseudomonads are 0.25 × 10 effectively containing bacterium number⁵Individual/mL, P.corrugata pseudomonad effectively contains bacterium Number is 0.32 × 10⁴Individual/mL；

The activation condition of E.cancerogenus enterobacterias is to take 1mL E.cancerogenus enterobacterias to be inoculated in be equipped with The constant-temperature table culture 20 hours of 32 DEG C of 15mL activation mediums；The activation culture based formulas is activation medium tryptone 10g/L, extract of soybean 5g/L, ammonium sulfate 3g/L, potassium dihydrogen phosphate 0.3g/L, pH 7.5；

It is respectively to take 1mL to take 1mL P.amygdali pseudomonads, the activation condition of P.corrugata pseudomonads P. amygdali pseudomonads, P.corrugata pseudomonads are inoculated in equipped with 35 DEG C of constant temperature in 15mL activation mediums respectively Shaking table culture 25 hours；The activation culture based formulas is gelatin peptone 12.0g, tryptone 8.0g, extract of soybean 8g, sulphur Sour ammonium 3g, potassium dihydrogen phosphate 0.2g, potassium sulfate 10.0g, magnesium chloride 1.4g, agar 15.0g, pH 7.3；

(3) interplant：By growing way stalwartness, high 18 centimetres of mulberry saplings of well developed root system while carrying out purple napier grass and filling the gaps with seedlings On the position among every row purple napier grass, the spacing in the rows of mulberry saplings is 300 centimetres of 120 cm x for interplanting；

(4) field management：

A, foliar fertilizer, dose 150 are sprayed to purple napier grass after high 30 centimetres of the seedling that purple napier grass seedling stem is grown Kg/acre；Foliar fertilizer is by mulberry tree dead leaf be soaked in water 2 days after fermentation is rotted, and filters to take to obtain nutrient solution, according to weight Number meter is measured by 50 parts of nutrient solution, 10 parts of ammonium sulfate, 8 parts of urea, 8 parts of potassium sulfate, 8 parts of potassium dihydrogen phosphate, aliphatic acid polyoxy second 2 parts of 0.5 part of alkenyl esters, 40 parts of water, 1 part of pull open powder, 1 part of polycarboxylate, xanthans mixing can obtain；

B, weeding processing is carried out to planting site herbicide spraying, the amount of spraying is up to after 40-50 centimetres after purple napier grass 60 kgs/acre；

C, 3 days after weeding processing, it is that the center of circle digs wide 10 centimetres of 5 centimetres of depths with 20 centimetres apart from purple napier grass stem Fertilising circle, then apply top dressing, every dose is 0.6 kilogram, after paramount 30 centimetres of mulberry tree length with trunk 30 centimetres be The center of circle digs 20 centimetres of fertilising circles of wide 20 centimetres of depths, then applies top dressing, and every dose is 0.8 kilogram, the top dressing according to Percetage by weight, weigh the 13% corn dregs of rice, 10% soya bean shell, 10% phosphorus mine tailing, 15% bagasse, 15% lotus stalk, 5% Carried out after vanilla Aqueous extracts, 5% strawberry Aqueous extracts, 10% bacillus subtilis, 8% Lactobacillus plantarum, the mixing of 8% cellulomonas Fermentation, the temperature for controlling fermentation are 35 DEG C, and ferment at constant temperature can obtain top dressing after 10 days；

D, pest repellant was sprayed to mulberry tree, purple napier grass at interval of 10 days, the amount of spraying is 60 kgs/acre；The pest repellant is Used after converting 20 parts of water after 8 parts of 10 parts of Galium verum, 5 parts of argy wormwood, 8 parts of peppermint, 8 parts of aloe, capsicum mixing；

After wherein capsicum crushes chilli, reach 60% using 0.5 times of addition water immersion 2 hours to chilli moisture content More than, be subsequently added into 2 times of volume mass fractions be 75% ethanol solution at 30 DEG C refluxing extraction 30 minutes, extract 3 altogether It is secondary, merge the filtrate of 3 filterings；

(5) gather in：Gathered in after purple napier grass is grown to 110 centimetres, field pipe is repeated after purple napier grass again long new talent The step of reason.

Control group 1：

It is same as Example 2, difference be the top dressing according to percetage by weight, weigh the 13% corn dregs of rice, 10% soya bean Shell, 10% phosphorus mine tailing, 15% bagasse, 25% lotus stalk, 10% bacillus subtilis, 8% Lactobacillus plantarum, 8% fiber Fermented after bacillus mixing, the temperature for controlling fermentation is 35 DEG C, and ferment at constant temperature can obtain top dressing after 10 days.

Control group 2：

It is same as Example 2, difference be the top dressing according to percetage by weight, weigh the 13% corn dregs of rice, 10% soya bean Shell, 10% phosphorus mine tailing, 15% bagasse, 20% lotus stalk, 5% strawberry Aqueous extracts, 10% bacillus subtilis, 8% plant Fermented after lactobacillus, the mixing of 8% cellulomonas, the temperature for controlling fermentation is 35 DEG C, and ferment at constant temperature is i.e. available after 10 days Top dressing.

Control group 3：

It is same as Example 2, difference be the top dressing according to percetage by weight, weigh the 13% corn dregs of rice, 10% soya bean Shell, 10% phosphorus mine tailing, 15% bagasse, 20% lotus stalk, 5% vanilla Aqueous extracts, 10% bacillus subtilis, 8% plant Fermented after lactobacillus, the mixing of 8% cellulomonas, the temperature for controlling fermentation is 35 DEG C, and ferment at constant temperature is i.e. available after 10 days Top dressing.

To embodiment 1, control group 1-3, blank control (ck), top dressing is carried out, and every piece of one mu of ground selection is compareed, and is examined Survey full phosphorus and 0.05mol/L NaHCO are used using sour molten-molybdenum antimony resistance colorimetric method, rapid available phosphorus₃- molybdenum antimony resistance colorimetric method, in addition The rapid available phosphorus contained under 5 plants of purple napier grasses, 5 plants of mulberry treies progress stem soil is randomly selected behind 24 hours and 48 hours after top dressing The content for the rapid available phosphorus that content, the stem of purple napier grass contain, is shown in Table 2：

Table 2:

As seen from the above table, the content of the rapid available phosphorus in soil of control group 1 is decomposed most fast, to the ＞ control groups 3 of ck ＞ control groups 2 ＞ embodiments 2, and in terms of the rapid available phosphorus on stem, the rapid available phosphorus height absorbed compares for the ＞ of 2 ＞ control groups of embodiment 2 Group 3 ＞ control groups 1 ＞ ck；The activity of P elements can be improved using the top dressing of the application by demonstrating, and prevent P elements easily by soil Oxidative deformation precipitation is not absorbed and used by plants.

Embodiment 3：

(1) purple napier grass seedling stem is handled：Ripe purple napier grass seedling stem is chopped to one section of a section, selects a two bud seedling stems of section, Then seedling stem is dried in the air using 1 minute taking-up nature of antimicrobial fluid immersion and put,

The antimicrobial fluid is mixed by following raw material according to parts by weight, 3 parts of Fructus Corni extract solution, Coreopsis tinctoria extract solution 5 parts, 4 parts of bur beggar-ticks extract solution, 90g/L brown sugar, 12g/L compound sodium nitrophenolates；

Wherein Fructus Corni extract solution is to dry Fructus Corni bark after rear ultramicrowave crushes to add, and adds the matter of 2 times of volumes Measure the ethanol solution that concentration is 75% and carry out alcohol extracting 4 minutes, microwave radiation technology alcohol extracting is carried out during alcohol extracting, the power of microwave is 380W, the first filtrate is taken after being filtered after alcohol extracting, take 150 milliliter of first filtrate to add 2 grams of amylase, carry out Soxhlet refluxing extraction 3 Secondary to filter to take the second filtrate, each 35 DEG C of constant temperature extracts 10 minutes, the second filtrate of filter 23 time is merged and can obtain mountain Zhu Cornel extract solution；

Wherein Coreopsis tinctoria extract solution is that the water that 4 times of volumes are added after fresh Coreopsis tinctoria flower is smashed to pieces is carried out after decocting 25 minutes Rotating centrifugal separation is carried out, centrifuge RPMs 2000r/min, centrifuges 3 minutes, isolates solid matter, goes filtrate, is carried out 3 times Decoct, rotating centrifugal separation, the filtrate of 3 times is merged, the filtrate after merging is then subjected to three layers of filtered through gauze, repeats 2 Secondary, merging filtrate can obtain Coreopsis tinctoria extract solution；

Wherein bur beggar-ticks extract solution is that 10 mesh sieves are crushed after bur beggar-ticks herb is dried, and adds 4 times of water and carries out 34 DEG C water-bath refluxing extraction filters to take filtrate after 1.5 hours, refluxing extraction 2 times, merge bur beggar-ticks filtrate, filter residue is added into pectin Enzyme, cellulase, water carry out adverse current extraction, and Extracting temperature is 33 DEG C, and extraction time is 40 minutes, obtains bur beggar-ticks filter residue liquid, Bur beggar-ticks filtrate and bur beggar-ticks filter residue liquid are mixed and can obtain bur beggar-ticks extract solution；

(2) site preparation：Cave is opened in the fertile field of selection soil property, and the spacing in the rows per cave is 220 centimetres of 23 cm x, before bunch planting kind Apply base manure, per 0.1 kilogram of cave, then purple napier grass seedling stem played and put with ditch, digging the covering of purple napier grass seedling stem, Watering to water content is 60%, and every plant of spacing of purple napier grass seedling stem of often row sowing is 30-40 centimetres, and sowing is observed after 10 days The growing way of seedling stem seedling, carry out processing of filling the gaps with seedlings；

The base manure is prepared by following raw material：N P and K 15:15:15 composite fertilizer, E.cancerogenus intestines bars Bacterium, P. amygdali pseudomonads, P.corrugata pseudomonads, glucose are 100 according to weight ratio:0.02:0.02: 0.01:0.2 mixed after use；

Further illustrate, the E.cancerogenus enterobacterias are 0.42 × 10 effectively containing bacterium number⁴Individual/mL, P.amygdali pseudomonads are 0.18 × 10 effectively containing bacterium number⁵Individual/mL, P.corrugata pseudomonad effectively contains bacterium Number is 0.28 × 10⁴Individual/mL；

The activation condition of E.cancerogenus enterobacterias is to take 1mL E.cancerogenus enterobacterias to be inoculated in be equipped with The constant-temperature table culture 19 hours of 32 DEG C of 15mL activation mediums；The activation culture based formulas is activation medium tryptone 7g/L, extract of soybean 4g/L, ammonium sulfate 2g/L, potassium dihydrogen phosphate 0.2g/L, pH 7.6；

It is respectively to take 1mL to take 1mL P.amygdali pseudomonads, the activation condition of P.corrugata pseudomonads P. amygdali pseudomonads, P.corrugata pseudomonads are inoculated in equipped with 35 DEG C of constant temperature in 15mL activation mediums respectively Shaking table culture 24.5 hours；The activation culture based formulas be gelatin peptone 11.0g, tryptone 6.0g, extract of soybean 5g, Ammonium sulfate 1.5g, potassium dihydrogen phosphate 0.2g, potassium sulfate 10.0g, magnesium chloride 1.4g, agar 15.0g, pH 7.1 ± 0.2；

(3) interplant：By growing way stalwartness, high 15-18 centimetres of mulberry of well developed root system while carrying out purple napier grass and filling the gaps with seedlings Sapling is interplanted on the position among every row purple napier grass, and the spacing in the rows of mulberry saplings is 220 centimetres of 90 cm x；

(4) field management：

A, foliar fertilizer, dose 130 are sprayed to purple napier grass after high 25 centimetres of the seedling that purple napier grass seedling stem is grown Kg/acre；Foliar fertilizer is by mulberry tree dead leaf be soaked in water 1 day after fermentation is rotted, and filters to take to obtain nutrient solution, according to weight Number meter is measured by 40 parts of nutrient solution, 8 parts of ammonium sulfate, 7 parts of urea, 5 parts of potassium sulfate, 6 parts of potassium dihydrogen phosphate, aliphatic acid polyoxy second 0.7 part of 0.2 part of alkenyl esters, 35 parts of water, 0.5 part of pull open powder, 0.6 part of polycarboxylate, xanthans mixing can obtain；

B, weeding processing is carried out to planting site herbicide spraying, the amount of spraying is up to after 40-50 centimetres after purple napier grass 55 kgs/acre；

C, 2 days after weeding processing, it is that the center of circle digs wide 10 centimetres of 5 centimetres of depths with 20 centimetres apart from purple napier grass stem Fertilising circle, then apply top dressing, every dose is 0.4 kilogram, after paramount 30 centimetres of mulberry tree length with trunk 24 centimetres be The center of circle digs 20 centimetres of fertilising circles of wide 20 centimetres of depths, then applies top dressing, and every dose is 0.6 kilogram, the top dressing according to Percetage by weight, weigh the 15% corn dregs of rice, 11% soya bean shell, 11% phosphorus mine tailing, 16% bagasse, 17% lotus stalk, 8% Carried out after vanilla Aqueous extracts, 9% strawberry Aqueous extracts, 6% bacillus subtilis, 3% Lactobacillus plantarum, the mixing of 4% cellulomonas Fermentation, the temperature for controlling fermentation are 35 ± 2 DEG C, and ferment at constant temperature can obtain top dressing after 5-10 days；

D, pest repellant was sprayed to mulberry tree, purple napier grass at interval of 10 days, the amount of spraying is 54 kgs/acre；The pest repellant is Used after converting 14 parts of water after 6 parts of 6 parts of Galium verum, 4 parts of argy wormwood, 4 parts of peppermint, 7 parts of aloe, capsicum mixing；

After wherein capsicum crushes chilli, reached using 0.5 times of addition water immersion 1.3 hours to chilli moisture content More than 60%, be subsequently added into 2 times of volume mass fractions be 75% ethanol solution at 30 DEG C refluxing extraction 30 minutes, carry altogether Take 3 times, merge the filtrate of 3 filterings；

(5) gather in：Gathered in after purple napier grass is grown to 100 centimetres, field pipe is repeated after purple napier grass again long new talent The step of reason.

Experimental group 1：

Same as Example 3, difference is that the pest repellant is by by after 6 parts of 6 parts of Galium verum, 7 parts of aloe, capsicum mixing Used after converting 14 parts of water.

Experimental group 2：

Same as Example 3, difference is that the pest repellant is by by 6 parts of Galium verum, 4 parts of argy wormwood, 7 parts of aloe, capsicum 6 Used after converting 14 parts of water after part mixing.

Experimental group 3：

Same as Example 3, difference is that the pest repellant is by by after 4 parts of 6 parts of Galium verum, 4 parts of argy wormwood, peppermint mixing Used after converting 14 parts of water.

Experimental group 4：

Same as Example 3, difference is that the pest repellant is by by 6 parts of Galium verum, 4 parts of argy wormwood, 4 parts of peppermint, capsicum 6 Used after converting 14 parts of water after part mixing.

Experimental group 5：

Same as Example 3, difference is to spray pest repellant.

Embodiment 3, experimental group 1-3 are compared, start to spray the situation for recording and disease occur on April 25th, 2016, Specifically it is shown in Table 3 purple napier grass growing states, the Mulberry Diseases And Pests situation of table 4：

Table 3

Table 4

The pest repellant of unused the application is can be seen that from table 3-4, it is more universal the phenomenon of disease occur, and with group The disease-resistant scope of reduction of compound can greatly reduce；Compare from experimental group 1 and 2, argy wormwood, peppermint have the disease-resistant of promotion that get along Ability, compare from experimental group 3 and 4, aloe, capsicum have the disease-resistant ability for promotion of getting along.

Embodiment 4：

(1) purple napier grass seedling stem is handled：Ripe purple napier grass seedling stem is chopped to one section of two section, selects one section of two bud seedling stem, Then seedling stem is dried in the air using 0.8 minute taking-up nature of antimicrobial fluid immersion and put,

The antimicrobial fluid is mixed by following raw material according to parts by weight, 4 parts of Fructus Corni extract solution, Coreopsis tinctoria extract solution 7 parts, 4 parts of bur beggar-ticks extract solution, 95g/L brown sugar, 12g/L compound sodium nitrophenolates；

Wherein Fructus Corni extract solution is to dry Fructus Corni bark after rear ultramicrowave crushes to add, and adds the matter of 3 times of volumes Measure the ethanol solution that concentration is 78% and carry out alcohol extracting 3 minutes, microwave radiation technology alcohol extracting is carried out during alcohol extracting, the power of microwave is 350W, the first filtrate is taken after being filtered after alcohol extracting, take 180 milliliter of first filtrate to add 2 grams of amylase, carry out Soxhlet refluxing extraction 3 Secondary to filter to take the second filtrate, each 35 DEG C of constant temperature extracts 10 minutes, the second filtrate of filter 23 time is merged and can obtain mountain Zhu Cornel extract solution；

Wherein Coreopsis tinctoria extract solution is that the water that 4 times of volumes are added after fresh Coreopsis tinctoria flower is smashed to pieces is carried out after decocting 28 minutes Rotating centrifugal separation is carried out, centrifuge RPMs 2000r/min, centrifuges 4 minutes, isolates solid matter, goes filtrate, is carried out 3 times Decoct, rotating centrifugal separation, the filtrate of 3 times is merged, the filtrate after merging is then subjected to three layers of filtered through gauze, repeats 3 Secondary, merging filtrate can obtain Coreopsis tinctoria extract solution；

Wherein bur beggar-ticks extract solution is that 10 mesh sieves are crushed after bur beggar-ticks herb is dried, and adds 3-5 times of water and carries out 33 DEG C of water-bath refluxing extractions filter to take filtrate after 1.8 hours, refluxing extraction 3 times, merge bur beggar-ticks filtrate, filter residue is added into fruit Glue enzyme, cellulase, water carry out adverse current extraction, and Extracting temperature is 34 DEG C, and extraction time is 45 minutes, obtains bur beggar-ticks filter residue Liquid, bur beggar-ticks filtrate and bur beggar-ticks filter residue liquid are mixed and can obtain bur beggar-ticks extract solution；

The base manure is prepared by following raw material：N P and K 15:15:15 composite fertilizer, E.cancerogenus intestines bars Bacterium, P. amygdali pseudomonads, P.corrugata pseudomonads, glucose are 100 according to weight ratio:0.04:0.03: 0.02:0.2 mixed after use；

Further illustrate, the E.cancerogenus enterobacterias are 0.48 × 10 effectively containing bacterium number⁴Individual/mL, P.amygdali pseudomonads are 0.21 × 10 effectively containing bacterium number⁵Individual/mL, P.corrugata pseudomonad effectively contains bacterium Number is 0.30 × 10⁴Individual/mL；

The activation condition of E.cancerogenus enterobacterias is to take 1mL E.cancerogenus enterobacterias to be inoculated in be equipped with The constant-temperature table culture 19.5 hours of 32 DEG C of 15mL activation mediums；The activation culture based formulas is activation medium tryptose Peptone 8g/L, extract of soybean 4.5g/L, ammonium sulfate 2.5g/L, potassium dihydrogen phosphate 0.25g/L, pH 7.4 ± 0.2；

It is respectively to take 1mL to take 1mL P.amygdali pseudomonads, the activation condition of P.corrugata pseudomonads P. amygdali pseudomonads, P.corrugata pseudomonads are inoculated in equipped with 35 DEG C of constant temperature in 15mL activation mediums respectively Shaking table culture 24.6 hours；The activation culture based formulas be gelatin peptone 11.50g, tryptone 6.0g, extract of soybean 7g, Ammonium sulfate 2g, potassium dihydrogen phosphate 0.2g, potassium sulfate 10.0g, magnesium chloride 1.4g, agar 15.0g, pH 7.1 ± 0.2；

(3) interplant：By growing way stalwartness, high 15-18 centimetres of mulberry of well developed root system while carrying out purple napier grass and filling the gaps with seedlings Sapling is interplanted on the position among every row purple napier grass, and the spacing in the rows of mulberry saplings is 280 centimetres of 100 cm x；

(4) field management：

A, foliar fertilizer, dose 135 are sprayed to purple napier grass after high 28 centimetres of the seedling that purple napier grass seedling stem is grown Kg/acre；Foliar fertilizer is by mulberry tree dead leaf be soaked in water 1 day after fermentation is rotted, and filters to take to obtain nutrient solution, according to weight Number meter is measured by 38 parts of nutrient solution, 8 parts of ammonium sulfate, 6 parts of urea, 4 parts of potassium sulfate, 7 parts of potassium dihydrogen phosphate, aliphatic acid polyoxy second 1.5 parts of 0.4 part of alkenyl esters, 38 parts of water, 0.7 part of pull open powder, 0.9 part of polycarboxylate, xanthans mixing can obtain；

B, weeding processing is carried out to planting site herbicide spraying, the amount of spraying is up to after 40-50 centimetres after purple napier grass 57 kgs/acre；

C, 2-3 days after weeding processing, it is that the center of circle digs wide 10 lis of 5 centimetres of depths with 20 centimetres apart from purple napier grass stem The fertilising circle of rice, then applies top dressing, and every dose is 0.4 kilogram, with 28 centimetres of trunk after paramount 30 centimetres of mulberry tree length Wide 20 centimetres of depths, 20 centimetres of fertilising circles are dug for the center of circle, then apply top dressing, every dose is 0.6 kilogram, and the top dressing is pressed According to percetage by weight, weigh the 15% corn dregs of rice, 15% soya bean shell, 15% phosphorus mine tailing, 16% bagasse, 16% lotus stalk, 6% vanilla Aqueous extracts, 6% strawberry Aqueous extracts, 9% bacillus subtilis, 6% Lactobacillus plantarum, the mixing of 6% cellulomonas are laggard Row fermentation, the temperature for controlling fermentation are 35 ± 2 DEG C, and ferment at constant temperature can obtain top dressing after 5-10 days；

D, pest repellant was sprayed to mulberry tree, purple napier grass at interval of 10 days, the amount of spraying is 56 kgs/acre；The pest repellant is Used after converting 19 parts of water after 7 parts of 8 parts of Galium verum, 4 parts of argy wormwood, 5 parts of peppermint, 7 parts of aloe, capsicum mixing；

After wherein capsicum crushes chilli, reached using 0.5 times of addition water immersion 1.4 hours to chilli moisture content More than 60%, be subsequently added into 2 times of volume mass fractions be 75% ethanol solution at 30 DEG C refluxing extraction 30 minutes, carry altogether Take 3 times, merge the filtrate of 3 filterings；

Test group 1：

Step same as Example 4, difference are that foliar fertilizer is the Patent No. that will be purchased using market 201310670843.8 foliar fertilizer sprayed according to operating instruction.

The foliar fertilizer of embodiment 4, CK and test group 1 is sprayed, to (being sprayed within 1 day, 2 days, 3 days after spraying foliar fertilizer Apply 3 after foliar fertilizer days and do not apply top dressing) the leaf surface fertility of the foliar fertilizers of each 3 plants of purple napier grasses of random searching, increase in blade The nutrition ratio added, is shown in Table 5：

Table 5

As seen from the above table, using the foliar fertilizer of the application, comparing has more excellent absorptivity, and from blade surface Fertility：Increased nutrition ratio, ratio simultaneously differ in leaf surface, and the ＞ CK of 4 ＞ test groups of embodiment 1, illustrate the leaf of the application Leaven attached ability it is stronger；Do not apply foliar fertilizer in terms of CK, but the nutritional ingredient in blade also increases, and should be itself Plant carries out originally containing organic matter in soil before photosynthesis also has, and meets reasoning.

Test group 2:

Step same as Example 4, difference are extremely to contain N P and K 15 for base manure：15:15 composite fertilizer.

Test group 3:

Step same as Example 4, difference are for E.cancerogenus enterobacterias, P.amygdali vacations in base manure Monad, P.corrugata pseudomonads are without activation process.

Base manure is added to embodiment 4, test group 2,3, stochastical sampling field soil test nutrient, Ca contain before base manure is added To measure as 0.0614 ± 0.001mg/kg, Mg contents be 0.221 ± 0.005mg/kg, the content of organic matter is 0.512 ± 0.004%, Full N content is that 0.035 ± 0.003%, heavy metal (copper, lead, chromium) content is 2.184 ± 0.004 (mg/kg)；Exist respectively Nutrient after application base manure in 3 points of detection soil of grab sample before mulberry tree is interplanted, is shown in Table 6：

Table 6

From table 6, soil with organic matter, full nitrogen, calcium content, the difference of content of magnesium are little, the difference of content of beary metal compared with It is big and progressively deep content tails off, but it is uncertain whether absorbed or be decomposed by purple napier grass, in order to study going for heavy metal To before base manure is applied, randomly selecting 5 plants of purple napier grasses and detect its content content of beary metal, basal dressing detects it again after 2 days Content content of beary metal, is shown in Table 7：

Table 7

	Embodiment 4	Test group 2	Test group 3	Ck
					Before fertilising	0.005±0.001	0.006±0.001	0.007±0.002	0.007±0.002
After fertilising 2 days	0.012±0.002	0.935±0.001	0.062±0.001	1.014±0.001

Contrasted from table 6 and table 7, the strain in the base manure that the content of beary metal of only embodiment 4 is configured absorbs, and tries The scarce capacity of the absorption heavy metal of strain in group 3 is tested, proves still there is part heavy metal by plant compared with test group 2 Absorbed；The base manure that demonstrating just has the application has the more excellent ability for adsorbing heavy metal, can locate before mulberry tree is interplanted Reason absorption heavy metal element, without being absorbed by mulberry tree in growth period so that it is too high that mulberry leaves contain heavy metal, serious shadow The quality of silkworm is rung, and then improves the survival rate of silkworm, improves the yield of silk cocoon, reaches the purpose of increase income.

Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that come for one of ordinary skill in the art Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention Protect scope.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

1. a kind of purple napier grass and the interplanting method of mulberry tree, it is characterised in that：Specifically include following steps：

(1) purple napier grass seedling stem is handled：Ripe purple napier grass seedling stem is chopped to one section of two section, selects one section of two bud seedling stem, then Seedling stem is taken out nature and dried in the air using antimicrobial fluid immersion 0.5-1.5 minutes and is put,

The antimicrobial fluid is mixed by following raw material according to parts by weight, Fructus Corni extract solution 1-5 parts, Coreopsis tinctoria extract solution 3- 8 parts, bur beggar-ticks extract solution 3-5 parts, 80-100g/L brown sugar, 10-15g/L compound sodium nitrophenolates；

(2) site preparation：Cave is opened in the fertile field of selection soil property, and the spacing in the rows per cave is 200-300 centimetres of 20-30 cm x, per bunch planting Apply base manure before kind, per 0.1 kilogram of cave, then play to put by purple napier grass seedling stem and cover purple napier grass seedling stem with ditch, digging Lid, it is 60% to water to water content, and every plant of spacing of purple napier grass seedling stem of often row sowing is 30-40 centimetres, and sowing is seen after 10 days The growing way of seedling stem seedling is examined, carries out processing of filling the gaps with seedlings；

The base manure is prepared by following raw material：N P and K 15:15:15 composite fertilizer, E.cancerogenus enterobacterias, P.amygdali pseudomonads, P.corrugata pseudomonads, glucose are 100 according to weight ratio:0.01-0.05:0.02- 0.03:0.01-0.02:0.1-0.3 is used after being mixed；

(3) interplant：By growing way stalwartness, high 15-18 centimetres of mulberry saplings of well developed root system while carrying out purple napier grass and filling the gaps with seedlings On the position among every row purple napier grass, the spacing in the rows of mulberry saplings is 200-300 centimetres of 80-120 cm x for interplanting；

(4) field management：

A, foliar fertilizer, dose 100- are sprayed to purple napier grass after high 20-30 centimetres of the seedling that purple napier grass seedling stem is grown 150 kgs/acre；

B, weeding processing is carried out to planting site herbicide spraying, the amount of spraying is 50- up to after 40-50 centimetres after purple napier grass 60 kgs/acre；

C, 2-3 days after weeding processing, it is that the center of circle digs wide 10 centimetres of 5 centimetres of depths with 20 centimetres apart from purple napier grass stem Fertilising circle, then applies top dressing, and every dose is 0.3-0.6 kilograms；

After mulberry tree length it is paramount after 30 centimetres with trunk 20-30 centimetres be that the center of circle digs wide 20 centimetres of depths, 20 centimetres of fertilising circles, then Applying top dressing, every dose is 0.5-0.8 kilograms,

(5) gather in：Gathered in after purple napier grass is grown to 100-110 centimetres, field pipe is repeated after purple napier grass again long new talent The step of reason.

2. a kind of purple napier grass according to claim 1 and the interplanting method of mulberry tree, it is characterised in that：The top dressing be by What following methods were prepared：According to percetage by weight, the 10%-15% corns dregs of rice, 10%-15% soya beans shell, 10%- are weighed 15% phosphorus mine tailing, 15%-20% bagasse, 15%-20% lotuses stalk, 5%-10% vanillas Aqueous extracts, 5%-10% strawberries Fermented after Aqueous extracts, 5%-10% bacillus subtilises, 2%-8% Lactobacillus plantarums, the mixing of 3%-8% cellulomonas, The temperature for controlling fermentation is 35 ± 2 DEG C, and ferment at constant temperature can obtain top dressing after 5-10 days.

3. a kind of purple napier grass according to claim 1 and the interplanting method of mulberry tree, it is characterised in that：The foliar fertilizer is It is prepared by following methods：Mulberry tree dead leaf be soaked in water 1-2 days after fermentation is rotted, filters to take to obtain nutrient solution, In parts by weight by nutrient solution 30-50 parts, ammonium sulfate 5-10 parts, urea 3-8 parts, potassium sulfate 3-8 parts, potassium dihydrogen phosphate 3- 8 parts, aliphatic acid polyethenoxy base ester 0.1-0.5 parts, water 30-40 parts, pull open powder 0.1-1 parts, polycarboxylate 0.1-1 parts, xanthan The mixing of glue 0.1-2 parts can obtain foliar fertilizer.

4. a kind of purple napier grass according to claim 1 and the interplanting method of mulberry tree, it is characterised in that：The pest repellant is pressed It is prepared according to parts by weight meter by following raw material：Galium verum 5-10 parts, argy wormwood 3-5 parts, peppermint 3-8 parts, aloe 5-8 Part, capsicum 5-8 parts.

5. a kind of purple napier grass according to claim 1 and the interplanting method of mulberry tree, it is characterised in that：The Fructus Corni carries It is to dry Fructus Corni bark after rear ultramicrowave crushes to add to take liquid, and the mass concentration for adding 2-3 times of volume is 70%-80%'s Ethanol solution carries out alcohol extracting 3-5 minutes, microwave radiation technology alcohol extracting is carried out during alcohol extracting, the power of microwave is 350-400W, after alcohol extracting The first filtrate is taken after filtering, takes the filtrate of 100-200 milliliters first to add 1-3 grams of amylase, carry out 3 filterings of Soxhlet refluxing extraction The second filtrate is taken, each 35 DEG C of constant temperature extracts 10 minutes, the second filtrate of filter 23 time is merged and can obtain Fructus Corni extraction Liquid.

6. a kind of purple napier grass according to claim 1 and the interplanting method of mulberry tree, it is characterised in that：The Coreopsis tinctoria carries It is that the water that 3-5 times of volume is added after fresh Coreopsis tinctoria flower is smashed to pieces carries out carrying out rotating centrifugal point after decocting 20-30 minutes to take liquid From, centrifuge RPMs 2000r/min, centrifuge 2-5 minutes, isolate solid matter, go filtrate, carry out 2-3 decoction, rotate from The heart is separated, and the filtrate of 3 times is merged, and the filtrate after merging then is carried out into three layers of filtered through gauze, is repeated 2-3 times, merging filtrate It can obtain Coreopsis tinctoria extract solution.

7. a kind of purple napier grass according to claim 1 and the interplanting method of mulberry tree, it is characterised in that：The bur beggar-ticks carries It is that 10 mesh sieves are crushed after bur beggar-ticks herb is dried to take liquid, adds 3-5 times of water and carries out 32-35 DEG C of water-bath refluxing extraction 1- Filtrate is filtered to take after 2 hours, refluxing extraction 2-3 times, merges bur beggar-ticks filtrate, filter residue is added into pectase, cellulase, water enters Row adverse current is extracted, and Extracting temperature is 32-35 DEG C, and extraction time is 30-50 minutes, obtains bur beggar-ticks filter residue liquid, bur beggar-ticks is filtered Liquid and the mixing of bur beggar-ticks filter residue liquid can obtain bur beggar-ticks extract solution.