CN107868842A - For detecting SNP marker and the application of rice brown planthopper resistant Bph3 genes - Google Patents
For detecting SNP marker and the application of rice brown planthopper resistant Bph3 genes Download PDFInfo
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- CN107868842A CN107868842A CN201711262413.7A CN201711262413A CN107868842A CN 107868842 A CN107868842 A CN 107868842A CN 201711262413 A CN201711262413 A CN 201711262413A CN 107868842 A CN107868842 A CN 107868842A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
The present invention is provided to detect brown planthopper resistant gene in rice Bph3 SNP marker and application.The invention discloses the SNP marker K_040503 isolated with brown planthopper resistant gene in rice Bph3 and expanding effect is good, the mark detection bit base of Chromosome 4 in Rice 6903106, polymorphism C/G.Based on the mark K_040503 primer sequences of KASP technological development as shown in SEQ ID No.1 3.The SNP marker detection Bph3 gene locis of the present invention are the site of high specific, can convenient and efficient be used for identifying in rice varieties whether contain Bph3 genes.The application process of SNP marker provided by the invention is accurately and reliably, easy to operate, identification and assisted selection suitable for Bph3 genes.
Description
Technical field
The present invention relates to technical field of molecular biology, and in particular to brown planthopper resistant gene in rice Bph3 SNP molecule marks
The method remembered and detect the molecular labeling.
Background technology
Rice is the important cereal crops in China.Brown paddy plant hopper is a kind of rice monophagy insect, sucks bast by lancet
Portion's juice is caused harm rice strain, cause paddy growth slowly, tiller delay, empty flat grain increase.Brown paddy plant hopper or grass-like bushy stunt virus and
The communication media of some Rice Virus such as tingia dwarf virus, have a strong impact on the production and safety of rice.At present, to brown paddy plant hopper
Preventing and treating depends on chemical prevention, not only adds the drug resistance of production cost and insect, and pollutes environment, therefore, profit
It is considered as to prevent and treat the effective way of its harm to cultivate brown planthopper resistant kind with host resistance.
It is up to the present, identified and report at least 34 brown planthopper resistant gene sites, wherein dominant gene 19,
Recessive gene 15, main effect brown planthopper resistant gene after positioning there are 4 resistant genes to be cloned up to 28, be Bph3 respectively
(Liu et al.2014), Bph14 (Du et al.2009), Bph9 (Zhao et al.2016) and Bph26 (Ji et
al.2016).Liu etc. has cloned dominant gene Bph3 from Sri Lanka locality rice variety Rathu heenati, the gene
Be one by 3 gene clusters for forming of coding plasma membrane agglutinin receptor kinases (OsLecRK1-OsLecRK3), while antibiont type
Ith, II, III and IV, be resistance is most wide so far brown planthopper resistant gene (Wang Hui, 2016；National rice data center).
Due to pest-resistant phenotypic evaluation process very complicated, the breeding efficiency of rice brown planthopper resistant kind is limited, and is utilized
Conventional breeding means are often difficult to effectively polymerize different anti insect genes.Exploitation and anti insect gene close linkage isolate
Molecular labeling, it polymerize one or more using molecular marker assisted selection (Marker-assisted selection, MAS) technology
Individual target gene or QTL, so as to seed selection durable resistance kind, delay the degeneration time limit of pest-resistant cultivar and prevent brown paddy plant hopper neoformation
The generation of type.
Traditional rice breeding for pest resistance be by Resistance Identification to plant carry out Phenotypic Selection, expend the time length, and easily by
The limitation of environmental condition, qualification result easily cause error, and efficiency of selection is relatively low.It is simple using molecular marker assisted selection breeding
Effectively, breeding cost can be reduced, shortens the seed selection cycle, can also carry out autotelic multiple gene polymerization, improves breeding efficiency, band
Carry out huge economic results in society.The type mainly utilized in document report is that SSR and InDel is marked, and is deposited in breeding
It is relatively low in polymorphic rate, the less shortcoming of difference.The EB or Polyscrylamide used in detection process is easily polluted to environment, is right
Human body produces harm.Exploitation is with the brown planthopper resistant gene Bph3 specific molecular markers isolated and establishing efficient, environment-friendly
Coherent detection system, then to promoting application of the Bph3 genes in Hybrid breeding in commercial system significant.Base based on KASP
Because classifying method is to be recorded by computer and fluorescence signal caused by analyzing during PCR, realizes and mutational site is monitored.Inspection
It is high with phenotype uniformity to survey result；Detection process without electrophoresis, thoroughly prevented PCR primer Aerosol Pollution and EB to ring
Harm of the pollution, formaldehyde in border to human body.
The content of the invention
It is an object of the invention to provide a kind of SNP marker for being used to detect brown planthopper resistant gene in rice Bph3 and answer
With.
In order to realize the object of the invention, technical scheme is as follows：According to the location information of gene, brown planthopper resistant base
Because Bph3 is located at Rice Chromosome 4 6939046-6969039 sections, centered on gene interval respectively expanding 50kb to both sides carries
SNP site is taken, and whether there are other SNP sites etc. to be selected according to PIC values and SNP site periphery 50bp.Meanwhile according to
Bph3 gene orders through clone are compared with Nipponbare sequence, the SNP site picked out, using BatchPrimer3 to it
Carry out design of primers.For these SNP markers, to containing Bph3 genetic donor material RH, IR56 and other more without Bph3
Individual rice varieties carry out KASP and react checking, and picking out specific can distinguish the resistant donor material simultaneously good SNP marks of expanding effect
Remember K_040503.Nature is carried out to the chain SNP marker K_040503 of resistant gene selected by 95 parts of natural population's materials
Colony verifies that it is rare bases to show resistance bases.
The invention provides a kind of SNP marker K_040503 for detecting brown planthopper resistant gene in rice Bph3, for examining
Survey base and be located at Chromosome 4 in Rice the 6903106th (MSU7.0 genomes version), the site is located at Bph3 gene intervals
At the 36Kb of upstream, the SNP marker polymorphism is C/G.
The above-mentioned SNP marker of the present invention is (1) two specific primer of specific primer sets：
PrimerX：5’-AAGCATTGGCATCAATAAGC-3’；
PrimerY：5’-AAGCATTGGCATCAATAAGG-3’
(2) universal primers：
PrimerC：5’-GGACACCCCTATTGGCTATT-3’.
The invention provides the specific primer sets for detecting above-mentioned SNP marker, including：
(1) two specific primer：
PrimerX：5’-AAGCATTGGCATCAATAAGC-3’；
PrimerY：5’-AAGCATTGGCATCAATAAGG-3’
(2) universal primers：
PrimerC：5’-GGACACCCCTATTGGCTATT-3’.
Reagent or kit containing above-mentioned specific primer sets belong to protection scope of the present invention.
The invention provides application of the above-mentioned specific primer sets in brown planthopper resistant gene in rice Bph3 is detected.
Described application, further comprises the steps：
(1) oryza sativa genomic dna to be measured is extracted；
(2) using oryza sativa genomic dna as template, KASP reaction detections are carried out using above-mentioned specific primer sets；
(3) if only detecting primer PrimerX corresponds to bases G, judge that detection rice sample is free of Bph3 genes；If only
Detect that primer PrimerY corresponds to base C, then judge that rice to be measured contains the Bph3 genes of homozygosis；If it is detected simultaneously by base C
And G, then judge the Bph3 genes that rice to be measured contains heterozygosis.
PCR conditions are in KASP detections in step (2)：94℃15min；94 DEG C of 20sec, 65-57 DEG C of 1min, each circulation
Annealing temperature reduces by 0.8 DEG C, totally 10 circulations；94 DEG C of 20sec, 57 DEG C of 1min, totally 26 circulations.
The invention provides the method for detection rice brown planthopper resistant Bph3 genotype, comprise the following steps：
(1) oryza sativa genomic dna to be measured is extracted；
(2) using oryza sativa genomic dna as template, KASP reaction detections are carried out using above-mentioned specific primer sets；
(3) if only detecting primer PrimerX corresponds to bases G, judge that detection rice sample is free of Bph3 genes；If only
Detect that primer PrimerY corresponds to base C, then judge that rice to be measured contains the Bph3 genes of homozygosis；If it is detected simultaneously by base C
And G, then judge the Bph3 genes that rice to be measured contains heterozygosis.
The invention provides applications of the SNP marker K_040503 in auxiliary identifies rice Bph3 genes.
The invention provides SNP marker K_040503 or above-mentioned specific primer sets or contain the specific primer
Application of the kit of combination in Rice Germplasm Resources improvement.
The invention provides SNP marker K_040503 or above-mentioned specific primer sets or contain the specific primer
Application of the kit of combination in cultivating with brown planthopper resistant ability rice.
The present invention can be high-throughout using the mark of the single base difference design based on KASP reaction principles and anti-sense material
Bph3 resistant gene detections are carried out to rice material, each mark is made up of three primers, two specific primers in embodiment
Fluorescent linker sequence corresponding to KASP reaction reagents, a universal primer are connected respectively.If it is corresponding to only detect primer PrimerX
Bases G, then judge that detection rice sample is free of Bph3 genes；If only detecting primer PrimerY corresponds to base C, judgement is treated
Survey the Bph3 genes that rice contains homozygosis；If being detected simultaneously by base C and G, the Bph3 bases that rice to be measured contains heterozygosis are judged
Cause.By the detection of two kinds of different fluorescence signals, realize that the genotype of the SNP site to each detection sample judges, Neng Gouke
Take existing Morphological Identification method detection cycle length, need rich experiences and PCR, RAPD, PCR-RFLP, Southern to hybridize
The shortcomings of not high etc. detection method complex operation or detection sensitivity or specific not strong or repeated bad, thoroughly prevent
Pollution, formaldehyde harm to human body of the Aerosol Pollution and EB of PCR primer to environment, have easy, reliable, quick, special
Property strong, high sensitivity, environment-friendly remarkable advantage.The application process of SNP marker provided by the invention accurately and reliably, is grasped
Make easy, identification and assisted selection suitable for Bph3 genes.
Brief description of the drawings
Fig. 1 is the application SNP marker K_040503 development process figure.
Fig. 2 is the application SNP marker K_040503 in natural population's genotyping result.
The genetic locus that Fig. 3 is the application SNP marker K_040503 is verified.
Embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It is it will be appreciated that following real
Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this area
Art personnel can carry out various modifications and replacement in the case of without departing substantially from spirit of the invention and spirit to the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
The exploitation of the SNP marker of the rice Bph3 genes of embodiment 1
According to the location information of gene, brown planthopper resistant gene Bph3 is located at Rice Chromosome 4 6939046-6969039 areas
Between (MSU7.0).Respectively expand 50kb extraction SNP sites to both sides centered on gene interval, and according to PIC values and SNP site week
Whether side 50bp has other SNP sites etc. to be selected.Meanwhile according to the Bph3 gene orders cloned and Nipponbare sequence
It is compared, the SNP site picked out, design of primers is carried out to it using BatchPrimer3.It is right for these SNP markers
KASP reaction checkings are carried out containing Bph3 genetic donor material RH, IR56 and other multiple rice varieties without Bph3, are selected
Go out to isolate with resistant donor material and SNP marker K_040503 that expanding effect is good.Above-mentioned SNP marker development process figure is shown in figure
1。
The primer of the present invention for being used to detect above-mentioned SNP marker is combined as：
(1) two specific primer：
PrimerX：5’-AAGCATTGGCATCAATAAGC-3’；
PrimerY：5’-AAGCATTGGCATCAATAAGG-3’
(2) universal primers：
PrimerC：5’-GGACACCCCTATTGGCTATT-3’.
Two the end of specific primer 5 ' connects the specific FAM and HEX fluorescent linkers sequence of LGC company's KASP reaction reagents respectively
Row.The synthesis of primer commission Invitrogen companies.
Table 1 marks K_040503 primer information
| ID | MSU7.0 positions | Deng bit base type X | Deng bit base type Y | Favourable base type |
| K_040503 | Chr04.6903106 | G | C | C |
KASP reactions are carried out to rice material genomic DNA to be measured.KASP reaction tests are in LGC SNPline Genotypings
Carried out on platform.20ng DNA samples are added in micro reaction plate, KASP reaction mixtures are added after drying, reaction system is shown in
Table 2.PCR amplifications are completed in water-bath thermal cycler, and Touchdown PCR reaction conditions are：94 DEG C of pre-degenerations 15 minutes；First
Amplified reaction is walked, 94 DEG C are denatured 20 seconds, and 65 DEG C~57 DEG C are annealed and extended 60 seconds, 10 circulations, each cycle annealing and extension
Temperature reduce by 0.8 DEG C；Second step amplified reaction, 94 DEG C are denatured 20 seconds, and 57 DEG C are annealed and extended 60 seconds, 26 circulations.Reaction
After the completion of using scanner Pherastar fluorescence data reading is carried out to KASP reaction products, the result of fluorescent scanning can be automatic
Change into figure.
The LGC SNPline Genotypings platforms used in the present invention are purchased from Britain LGC public affairs with its matched reagent consumptive material
Department.
The reaction system of the KASP of table 2 detections
| Final concentration | Volume (ul) | |
| 100UM Primer C | 0.42UM | 0.0125 |
| 100UM Primer X | 0.17UM | 0.0050 |
| 100UM Primer Y | 0.17UM | 0.0050 |
| 2×KASP Master Mix | 1× | 1.4792 |
| Ultra-pure water | 1.4983 | |
| Cumulative volume | 3 |
With mark K_040503 to rice varieties RH, IR56 containing Bph3 genes and other 28 rice without Bph3
Kind carries out KASP reaction checkings, as a result such as table 3.The rice varieties of the gene containing Bph3 detect alkali in K_040503 test sites
Base C, 27 parts equal in test site without Bph3 rice varieties either other brown planthopper resistant gene donors or susceptible material
Detect bases G.
Table 3 marks K_040503 primary dcreening operation typing datas
The application of the SNP marker of the rice Bph3 genes of embodiment 2
High-throughout rice material can be carried out using KASP reactions for molecular labeling of the present invention design anti-brown
Plant hopper Bph3 genetic tests, the primer designed using embodiment 1 are combined.SNP marker K_040503 is carried out using 95 parts of materials
Natural population verifies.95 parts of materials include feeling worm control material, common hybridization rice and core rice breed.Mark is certainly
Right colony's genotyping result is as shown in Fig. 2 in addition to association excellent 716 detects heterozygosis Bph3 genotype, other sense worm control materials, often
See that hybrid paddy rice and core rice breed are detected as no Brown Planthopper Resistance homozygosis bph3 genotype.It can be seen that SNP marker K_
040503 detection Bph3 gene locis are the resistance locus of high specific, can convenient and efficient be used to identify in rice varieties whether
Contain Bph3 genes.
The genetic locus of embodiment 3SNP marks and the checking of segregating population phenotype
1st, genetic locus checking is marked
Using 79 plants of Bph3 RIL colonies and 10 in Parent in SNP marker and the present invention with polymorphism
Embodiment 1 develops obtained Bph3 specific markers K_040503 and carries out genetic locus checking, mark K_040503 of the invention
It is positioned to Chromosome 4 in Rice 0.8cM positions (see Fig. 3).
2nd, phenotype checking is marked
Using K_040503 is marked to 1327 (Bph3 donors) and C594 F2 colonies Genotyping, with SSR marker gene
Type data comparison, population material and SSR marker data come from grand flat high-tech, and as shown in table 4, the uniformity of two kinds of marks is preferable,
The feasibility and accuracy for the SNP marker detection method that the present invention develops, the mirror available for Bph3 genes are demonstrated again
Fixed and assisted selection.
Table 4
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Sequence table
<110>Hua Zhi rice biologicals Technology Co., Ltd.
<120>For detecting SNP marker and the application of rice brown planthopper resistant Bph3 genes
<130> KHP171115426.8
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 1
aagcattggc atcaataagc 20
<210> 2
<211> 20
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 2
aagcattggc atcaataagg 20
<210> 3
<211> 20
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 3
ggacacccct attggctatt 20
Claims (10)
- A kind of 1. SNP marker for being used to detect brown planthopper resistant gene in rice Bph3, it is characterised in that detection rice the 4th The bit base of chromosome the 6903106th, the site are located at Bph3 gene interval 6939046-6969039 upstream 36Kb, SNP molecules Mark polymorphism is C/G.
- 2. SNP marker as claimed in claim 1, it is following specific primer sets,(1) two specific primer：PrimerX：5’-AAGCATTGGCATCAATAAGC-3’；PrimerY：5’-AAGCATTGGCATCAATAAGG-3’；(2) universal primers：PrimerC：5’-GGACACCCCTATTGGCTATT-3’.
- 3. the specific primer sets of 1 or 2 SNP markers are required for test right, it is characterised in that including：(1) two specific primer：PrimerX：5’-AAGCATTGGCATCAATAAGC-3’；PrimerY：5’-AAGCATTGGCATCAATAAGG-3’；(2) universal primers：PrimerC：5’-GGACACCCCTATTGGCTATT-3’.
- 4. reagent or kit containing specific primer sets described in claim 3.
- 5. application of the specific primer sets in brown planthopper resistant gene in rice Bph3 is detected described in claim 3.
- 6. application according to claim 5, it is characterised in that comprise the following steps：(1) oryza sativa genomic dna to be measured is extracted；(2) using oryza sativa genomic dna as template, KASP reaction inspections are carried out using the specific primer sets described in claim 3 Survey；(3) if only detecting primer PrimerX corresponds to bases G, judge that detection rice sample is free of Bph3 genes；If only detect Base C is corresponded to primer PrimerY, then judges that rice to be measured contains the Bph3 genes of homozygosis；If being detected simultaneously by base C and G, Then judge the Bph3 genes that rice to be measured contains heterozygosis.
- 7. the application according to claim 5 or 6, it is characterised in that PCR conditions are in KASP detections in step (2)：94℃ 15min；94 DEG C of 20sec, 65-57 DEG C of 1min, each cycle annealing temperature reduce by 0.8 DEG C, totally 10 circulations；94 DEG C of 20sec, 57 DEG C 1min, totally 26 circulations.
- 8. application of the SNP marker in auxiliary identifies rice Bph3 genes described in claim 1 or 2.
- 9. the specific primer sets or claim 4 described in SNP marker or claim 3 described in claim 1 or 2 Application of the described kit in Rice Germplasm Resources improvement.
- 10. the specific primer sets or claim described in SNP marker or claim 3 described in claim 1 or 2 Application of the kit in cultivating with brown planthopper resistant ability rice described in 4.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110423839A (en) * | 2019-08-21 | 2019-11-08 | 南宁维尔凯生物科技有限公司 | The SNP marker and its KASP detection method of rice brown planthopper resistant major gene resistance bph3 and application |
| CN110846432A (en) * | 2019-11-27 | 2020-02-28 | 广西壮族自治区农业科学院 | Codominant fluorescent molecular marker and detection method of brown planthopper resistant gene Bph3 |
| CN112458198A (en) * | 2020-12-17 | 2021-03-09 | 华智生物技术有限公司 | Auxiliary breeding molecular marker of brown planthopper resistant gene Bph27 and application thereof |
| CN114214448A (en) * | 2021-10-29 | 2022-03-22 | 袁隆平农业高科技股份有限公司 | SNP marker for identifying brown planthopper resistant gene Bph30 of rice and application thereof |
| CN114410623A (en) * | 2022-02-17 | 2022-04-29 | 武汉大学深圳研究院 | SNP molecular marker and application thereof in detection of brown planthopper resistant gene Bph44(t) of rice |
| CN114410623B (en) * | 2022-02-17 | 2024-04-23 | 武汉大学深圳研究院 | SNP molecular marker and application thereof in detection of brown planthopper resistant gene Bph44 (t) of rice |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101956019A (en) * | 2008-12-12 | 2011-01-26 | 南京农业大学 | Molecular marker of anti-nilaparvata-lugens major gene Bph3 of rice |
| KR101788989B1 (en) * | 2016-09-30 | 2017-10-24 | 충북대학교 산학협력단 | Marker for selecting brown planthopper-resistant rice cultivar and uses thereof |
-
2017
- 2017-12-04 CN CN201711262413.7A patent/CN107868842B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101956019A (en) * | 2008-12-12 | 2011-01-26 | 南京农业大学 | Molecular marker of anti-nilaparvata-lugens major gene Bph3 of rice |
| KR101788989B1 (en) * | 2016-09-30 | 2017-10-24 | 충북대학교 산학협력단 | Marker for selecting brown planthopper-resistant rice cultivar and uses thereof |
Non-Patent Citations (3)
| Title |
|---|
| ENSEMBL PLANTS: "vcZ28I8U3", 《ENSEMBL PLANTS》 * |
| YUQIANG LIU等: "A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice", 《NATURE BIOTECHNOLOGY》 * |
| 王慧等: "稻抗褐飞虱基因研究进展与展望", 《杂交水稻》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110423839A (en) * | 2019-08-21 | 2019-11-08 | 南宁维尔凯生物科技有限公司 | The SNP marker and its KASP detection method of rice brown planthopper resistant major gene resistance bph3 and application |
| CN110423839B (en) * | 2019-08-21 | 2022-12-02 | 南宁维尔凯生物科技有限公司 | SNP molecular marker of brown planthopper resistant major gene bph3 of rice as well as KASP detection method and application thereof |
| CN110846432A (en) * | 2019-11-27 | 2020-02-28 | 广西壮族自治区农业科学院 | Codominant fluorescent molecular marker and detection method of brown planthopper resistant gene Bph3 |
| CN112458198A (en) * | 2020-12-17 | 2021-03-09 | 华智生物技术有限公司 | Auxiliary breeding molecular marker of brown planthopper resistant gene Bph27 and application thereof |
| CN114214448A (en) * | 2021-10-29 | 2022-03-22 | 袁隆平农业高科技股份有限公司 | SNP marker for identifying brown planthopper resistant gene Bph30 of rice and application thereof |
| CN114214448B (en) * | 2021-10-29 | 2023-10-13 | 袁隆平农业高科技股份有限公司 | SNP marker for identifying brown planthopper resistant gene Bph30 of rice and application thereof |
| CN114410623A (en) * | 2022-02-17 | 2022-04-29 | 武汉大学深圳研究院 | SNP molecular marker and application thereof in detection of brown planthopper resistant gene Bph44(t) of rice |
| CN114410623B (en) * | 2022-02-17 | 2024-04-23 | 武汉大学深圳研究院 | SNP molecular marker and application thereof in detection of brown planthopper resistant gene Bph44 (t) of rice |
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| CN107868842B (en) | 2021-07-06 |
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