CN112592997B - Auxiliary breeding molecular marker of brown planthopper resistant gene Bph9 and application thereof - Google Patents
Auxiliary breeding molecular marker of brown planthopper resistant gene Bph9 and application thereof Download PDFInfo
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- C12Q2600/00—Oligonucleotides characterized by their use
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Abstract
The invention provides an auxiliary breeding molecular marker of brown planthopper resistant gene Bph9 and application thereof. The invention discloses a SNP marker K120504 which is co-separated from a brown planthopper resistant gene Bph9 of rice and has good amplification effect, the marker detects 22886030 bit base (MSU 7.0) of chromosome 12 of rice, polymorphism is T/G, and a primer sequence of marker K120504 developed based on KASP technology is shown as SEQ ID No: 1-3. The SNP molecular marker can be used for detecting that Bph9 gene locus is a locus with high specificity, and can be used for conveniently and efficiently identifying whether the rice variety contains the Bph9 gene. The application method of the SNP molecular marker provided by the invention is accurate and reliable, is simple and convenient to operate, and is suitable for identifying Bph9 genes and assisting in selective breeding.
Description
Technical Field
The invention relates to the technical fields of molecular biology and crop breeding, in particular to an auxiliary breeding molecular marker of a brown planthopper resistant gene Bph9 and application thereof.
Background
Rice is an important grain crop in China. Brown planthoppers are rice monophagic pests, and the brown plant hoppers absorb phloem juice through a mouth needle to serve as harmful rice plants, so that the rice grows slowly, tillers are delayed, and empty and shrunken grains are increased. Brown planthoppers are also a transmission medium of rice viruses such as grass-like plexus dwarf virus, dentate leaf dwarf virus and the like, and seriously affect the production and safety of rice. At present, the control of brown planthoppers mainly depends on chemical control, not only increases the production cost and the drug resistance of pests, but also pollutes the environment, so that the cultivation of brown planthopper resistant varieties by utilizing host resistance is considered as an effective way for controlling the harm of brown planthoppers.
To date, at least 34 brown planthopper resistant gene loci have been identified and guided, 19 of which dominant genes, 15 of which recessive genes, up to 28 of which have been located, and 4 of which resistance genes have been cloned, bph3 (Liu et al 2014), bph14 (Du et al 2009), bph9 (Zhao et al 2016) and Bph26 (jet et al 2016), respectively. The Kaharamana rice variety has insect resistance to brown planthoppers in east Asia and southeast Asia, su et al utilize SSR markers to locate an insect-resistant gene Bph9 in Kaharamana between markers RM463 and RM5341 on chromosome 12, zhao clones and analyzes the Bph9, the BPH9 locus is a gene cluster on a long arm of the chromosome 12, the locus has 8 alleles, and Bph1, bph2, bph7, bph18 and the like which are the most widely applied alleles of the BPH 9.
Because the insect-resistant phenotype identification process is complicated, the breeding efficiency of brown planthopper resistant varieties of rice is limited, and different insect-resistant genes are difficult to effectively polymerize by utilizing a conventional breeding means. Developing molecular markers closely linked or co-separated with insect-resistant genes, and polymerizing one or more target genes or QTL (quantitative trait locus) by using molecular Marker-assisted selection (Marker-assisted selection, MAS) technology, thereby breeding durable resistant varieties, delaying the degeneration period of the insect-resistant varieties and preventing the occurrence of new-born brown planthoppers
The traditional rice insect-resistant breeding is to perform phenotype selection on plants through resistance identification, so that the time consumption is long, the rice insect-resistant breeding is easy to be limited by environmental conditions, the identification result is easy to cause errors, and the selection efficiency is low. The molecular marker is utilized to assist in selecting and breeding, so that the breeding cost can be reduced, the breeding period can be shortened, the purposeful polygene polymerization can be carried out, the breeding efficiency can be improved, and huge social and economic benefits can be brought. The main types of markers used in literature report are SSR and InDel markers, and the defects of low polymorphism rate and small difference exist in breeding. EB or polypropylene amide used in the detection process is easy to pollute the environment and cause harm to human body. The development of a specific molecular marker coseparated with the brown planthopper resistant gene Bph9 and the establishment of an efficient and environment-friendly related detection system are of great significance in promoting the application of the Bph9 gene in commercial breeding.
Disclosure of Invention
The invention aims to develop a molecular marker of a high-resistance, broad-spectrum and durable brown planthopper resistant gene Bph9, which can be used for identifying the Bph9 gene and assisting in selective breeding.
The development flow of the brown planthopper resistant gene Bph9 assisted breeding molecular marker is shown in figure 1. Earlier studies showed that the brown planthopper resistant gene Bph9 was derived from the indica rice variety Kaharamana and located between the two SSR markers RM463 and RM5341 on chromosome 12 of rice (national rice data center).
The physical position on the corresponding reference genome Japanese sunny (MSU 7.0) is determined by using the Bph9 gene sequence published in the earlier literature, and SNP loci on both sides of the gene interval and the vicinity thereof are mined. And (3) selecting SNP loci, extracting flanking sequences, and designing primers by using an online primer design website BatchPrimer 3.
For the candidate SNP markers, carrying out KASP preliminary screening reaction verification on a donor material Pokkali containing Bph9 genes, 20 parts of other insect-resistant gene donor materials, 2 parts of insect-sensitive control materials and 8 parts of common rice materials, and selecting SNP marker K120504 which is coseparated with the Bph9 donor materials and has good amplification effect.
And then, carrying out natural population verification on the selected resistance gene linked SNP markers by using about 162 parts of materials, and proving that the Bph9 gene locus detected by the invention is a high-specificity resistance locus and can be used for screening and detecting Bph 9.
In order to achieve the aim of the invention, the invention provides an auxiliary breeding molecular marker of the brown planthopper resistant gene Bph9, wherein the molecular marker is SNP marker K120504 coseparated with the brown planthopper resistant gene Bph9 of rice, and the SNP marker detects 22886030 th base of chromosome 12 of the rice.
The invention also provides a primer developed based on the KASP technology and used for identifying the brown planthopper resistant gene Bph9 of rice, which comprises a specific primer X, a specific primer Y and a universal primer C, wherein the primer sequences are respectively shown in SEQ ID NO: 1-3.
The invention also provides a detection reagent or a kit containing the primer.
The invention also provides application of the molecular marker, the primer, the detection reagent or the kit in identifying the brown planthopper resistant gene Bph9 of rice.
The invention also provides application of the molecular marker, the primer, the detection reagent or the kit in auxiliary breeding of the brown planthopper resistant gene Bph 9.
The invention also provides application of the molecular marker, the primer, the detection reagent or the kit in breeding rice resources with brown planthopper resistance.
The application comprises the following steps:
1) Extracting DNA of a rice sample to be detected;
2) 20ng of dried template DNA,100UM specific primer X0.005. Mu.L, 100UM specific primer Y0.005. Mu.L, 100UM universal primer C0.0125. Mu.L, 2 XKASP Master Mix 1.4792. Mu.L, H were taken 2 O1.4983. Mu.L, and performing PCR amplification;
3) And analyzing the genotype of the PCR amplified product by adopting a fluorescence detector.
Further, the PCR reaction conditions in step 2) are: pre-denaturation at 94 ℃ for 15 min; first amplification reaction: denaturation at 94 ℃ for 20 seconds, annealing at 65-57 ℃ and extension for 60 seconds, 10 cycles, wherein the annealing and extension temperature is reduced by 0.8 ℃ in each cycle; the second amplification step was performed by denaturation at 94℃for 20 seconds, annealing at 57℃and extension for 60 seconds, 26 cycles.
Further, the step 3) specifically comprises: typing the PCR amplified product by using biological software, and if the PCR product of the sample only detects fluorescent signals corresponding to the specific primer X, determining that the detected rice sample is homozygous and not insect-resistant bph9 genotype, wherein the detection site is a base G; if only the fluorescent signal corresponding to the specific primer Y is detected, the detection site is a base T, and the rice sample to be tested is judged to be homozygous insect-resistant Bph9 genotype; if two fluorescent signals are detected simultaneously, the detection site is G: t, judging that the rice to be detected is heterozygous Bph9 resistant genotype. The allele K120504-T is a rice plant with excellent allele type of brown planthopper resistance.
By using the SNP marker K120504 provided by the invention, the allelic type of the Bph9 gene in the rice variety to be measured is finally confirmed by detecting the 'Bph 9 gene locus' of the rice variety.
The invention has the advantages of simple operation, low cost and short period, has good stability, is not influenced by other gene effects and environmental factors, can select in early generation, shortens the breeding period, improves the breeding efficiency, and is suitable for popularization and application. The invention has important significance for improving brown planthopper resistant varieties of rice, and is suitable for auxiliary selective breeding of Bph9 genes.
The KASP-based genotyping method is to record and analyze fluorescent signals generated in the PCR process by a computer so as to monitor mutation sites. The consistency of the detection result and the phenotype is high; electrophoresis is not needed in the detection process, so that aerosol pollution of PCR products and pollution of EB to the environment and harm of formaldehyde to human bodies are thoroughly eliminated.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and together with the description, serve to explain the principles of the invention.
In order to more clearly illustrate the embodiments of the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, and it will be obvious to a person skilled in the art that other drawings can be obtained from these drawings without inventive effort.
FIG. 1 is a flowchart of the development of the molecular marker for auxiliary breeding of the brown planthopper resistant gene Bph 9.
FIG. 2 is a diagram showing the detection of SNP marker K120504 in example 2 of the invention.
Detailed Description
In order that the above objects, features and advantages of the invention will be more clearly understood, a further description of the invention will be made. It should be noted that, without conflict, the embodiments of the present invention and features in the embodiments may be combined with each other.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced otherwise than as described herein; it will be apparent that the embodiments in the specification are only some, but not all, embodiments of the invention.
Preferred embodiments of the present invention will be described in detail below with reference to examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and alterations of this invention may be made by those skilled in the art without departing from the spirit and scope of this invention.
The experimental methods used in the following examples are conventional methods unless otherwise specified.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1 obtaining of molecular markers for auxiliary breeding of Brown planthopper resistant Gene Bph9 in Rice
The embodiment is used for explaining the application of the molecular marker provided by the invention in detecting the brown planthopper resistant gene Bph9, and the specific steps are as follows:
1. primer design
According to the related literature, the position of the brown planthopper resistant gene Bph9 is determined in a No. 12 chromosome 22872945-2288634 interval of Japanese sunny (MSU 7.0), the specific SNP locus and flanking sequences of the Bph9 gene in the interval are extracted, and an online primer design website BatchPrimer3 (http:// probes. Pw. Usda. Gov/patchPrimer 3 /) is utilized for primer design. Each group is marked with three primers, and FAM and HEX fluorescent sequences are respectively connected to the 5' ends of two specific primers. Primers were commissioned for Invitrogen corporation synthesis.
Table 1: molecular marker and primer information
The marker designed based on KASP reaction principle and single base difference of anti-sensitive material can detect Bph9 resistance gene of rice material with high flux, if only FAM fluorescence is detected in the sample, the base of the sample is AlleX; if only HEX fluorescence is detected, the base of the sample is Allle Y; if both fluorescence are detected simultaneously, the base at that site is heterozygous.
2. Extraction of genomic DNA from rice leaves by simplified CTAB method
(1) Sampling, putting the sample into 2.0mL Tube, adding two steel balls and 750 mu L of CTAB solution in advance, and shaking to homogenize the sample for 1.5min;
(2) heating at 65deg.C for 0.5-1 hr;
(3) cooling to room temperature, adding 750mL chloroform-isoamyl alcohol (24:1) solution into a fume hood, and mixing;
(4) centrifuging at 12000rmp for 10min, taking about 500mL of supernatant, and transferring into a new 1.5mL centrifuge tube;
(5) adding isopropanol solution with equal volume, mixing, precipitating at-20deg.C for more than 1 hr, centrifuging at 12000rmp for 10min, and removing supernatant;
(6) 1000mL of 70% ethanol is added, the mixture is flicked and precipitated, 1000rmp is centrifuged for 3min, and the supernatant is removed;
(7) 300 mu L H is added 2 O was dissolved overnight for use.
3. Kasp reaction test
The KASP response test was performed on the LGC SNPline genotyping platform. 20ng of DNA sample was added to the microwell reaction plate, dried and then KASP reaction mixture was added thereto, and the reaction system was shown in Table 2.
Table 2: KASP detected reaction system
| Final concentration | Volume (mu L) | |
| 100UM Primer C | 0.42μM | 0.0125 |
| 100UM Primer X | 0.17μM | 0.0050 |
| 100UM Primer Y | 0.17μM | 0.0050 |
| 2x KASP Master Mix | 1x | 1.4792 |
| Ultrapure water | 1.4983 | |
| Total volume of | 3 |
The PCR amplification is completed in a water bath thermal cycler, and the TouchDown PCR reaction conditions are as follows: pre-denaturation at 94 ℃ for 15 min; the first step of amplification reaction, denaturation at 94 ℃ for 20 seconds, annealing at 65-57 ℃ and extension for 60 seconds, 10 cycles, wherein the annealing and extension temperature of each cycle is reduced by 0.8 ℃; the second amplification step was performed by denaturation at 94℃for 20 seconds, annealing at 57℃and extension for 60 seconds, 26 cycles. After the reaction is completed, a scanner Pherastar is used for reading fluorescence data of KASP reaction products, and the result of fluorescence scanning can be automatically converted into a pattern.
The LGC SNpline genotyping platform and the matched reagent consumable used in the invention are purchased from LGC company in England.
4. Marking type data
KASP preliminary screening reaction verification was performed with marker K120504 on insect-resistant gene Bph9 donor material Pokkali, 20 parts of other insect-resistant gene donors, 2 parts of insect-susceptible controls and 8 parts of common rice material, and the results are shown in table 3. The donor variety of the Bph9 gene detects the insect-resistant genotype T at the K120504 test site, and the other 30 materials are all the insect-resistant genotype G.
Table 3: marked K120504 primary screening data
Example 2 application of SNP marker K120504 of brown planthopper resistant gene Bph9 of rice
In order to detect the specificity and practicability of the marker in the invention, 162 parts of material are utilized to carry out natural population verification on SNP marker K120504. 162 parts of materials comprise pest sensing control materials, common hybrid rice and core rice breeding materials. The typing results of the markers in the natural population are shown in FIG. 2, and except that the Pokkali, CR 157-392-284 and Pokkelli 13 detect the homozygous insect-resistant Bph9 genotype, other insect-sensitive control materials, common hybrid rice and core rice breeding materials are all detected as the brown planthopper-resistant homozygous Bph9 genotype. Therefore, SNP marker K120504 detects Bph9 gene locus as a high-specificity resistance locus, and can be used for conveniently and efficiently identifying whether rice varieties contain insect-resistant Bph9 genes.
The foregoing is only a specific embodiment of the invention to enable those skilled in the art to understand or practice the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown and described herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Sequence listing
<110> Huazhi Rice biotechnology Co., ltd
<120> molecular marker for auxiliary breeding of brown planthopper resistant gene Bph9 and application thereof
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<213> Artificial sequence (Artificial Sequence)
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<213> Artificial sequence (Artificial Sequence)
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Claims (4)
1. Application of a primer or a detection reagent or a kit in breeding rice resources with brown planthopper resistance, wherein the primer consists of a specific primer X, a specific primer Y and a universal primer C, and the primer sequences are respectively shown in SEQ ID NO:1-3, said reagent or kit comprising the above-mentioned primers, comprising the steps of:
1) Extracting DNA of a rice sample to be detected;
2) 20ng of dried template DNA was taken, 100. Mu.M of specific primer X0.005. Mu.L, 100. Mu.M of specific primer Y0.005. Mu.L, 100. Mu.M of universal primer C0.0125. Mu.L, 2 XKASP Master Mix 1.4792. Mu.L, H 2 O1.4983. Mu.L, and performing PCR amplification;
3) And analyzing the genotype of the PCR amplified product by adopting a fluorescence detector.
2. The use according to claim 1, wherein the PCR reaction conditions of step 2) are: pre-denaturation at 94 ℃ for 15 min; first amplification reaction: denaturation at 94 ℃ for 20 seconds, annealing at 65-57 ℃ and extension for 60 seconds, 10 cycles, wherein the annealing and extension temperature is reduced by 0.8 ℃ in each cycle; the second amplification step was performed by denaturation at 94℃for 20 seconds, annealing at 57℃and extension for 60 seconds, 26 cycles.
3. The use according to claim 1 or 2, characterized in that step 3) is in particular: the PCR amplification product is typed by using biological software, and the allelic site K120504-T is a rice plant with excellent allelic type of brown planthopper resistance.
4. The application of a primer or a detection reagent or a kit in auxiliary breeding of rice brown planthopper resistant gene Bph9, wherein the primer consists of a specific primer X, a specific primer Y and a universal primer C, and the primer sequences are respectively shown in SEQ ID NO:1-3, said reagent or kit comprising the above-mentioned primer.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667309A (en) * | 2012-09-05 | 2014-03-26 | 武汉大学 | Brown planthopper resistant rice gene Bph9 as well as molecular marker and application thereof |
| CN107488731A (en) * | 2017-09-30 | 2017-12-19 | 湖南隆平高科种业科学研究院有限公司 | Special SNP codominant markers primer and application in brown planthopper resistant gene in rice BPH9 genes |
| CN109554494A (en) * | 2018-12-13 | 2019-04-02 | 袁隆平农业高科技股份有限公司 | The general codominant marker and its detection method of rice brown planthopper resistant BPH9 multiple allele and application |
| CN109762929A (en) * | 2019-03-27 | 2019-05-17 | 江西省农业科学院水稻研究所 | A kind of Functional marker, identification method and the application of brown planthopper resistant gene in rice Bph9 |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667309A (en) * | 2012-09-05 | 2014-03-26 | 武汉大学 | Brown planthopper resistant rice gene Bph9 as well as molecular marker and application thereof |
| CN107488731A (en) * | 2017-09-30 | 2017-12-19 | 湖南隆平高科种业科学研究院有限公司 | Special SNP codominant markers primer and application in brown planthopper resistant gene in rice BPH9 genes |
| CN109554494A (en) * | 2018-12-13 | 2019-04-02 | 袁隆平农业高科技股份有限公司 | The general codominant marker and its detection method of rice brown planthopper resistant BPH9 multiple allele and application |
| CN109762929A (en) * | 2019-03-27 | 2019-05-17 | 江西省农业科学院水稻研究所 | A kind of Functional marker, identification method and the application of brown planthopper resistant gene in rice Bph9 |
Non-Patent Citations (1)
| Title |
|---|
| Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311;Cong Xiao等;Sci. Rep.;第6卷;38159 * |
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