CN114150082A - Development and application of KASP molecular marker of corn drought-tolerant gene - Google Patents
Development and application of KASP molecular marker of corn drought-tolerant gene Download PDFInfo
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- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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- C12Q2600/00—Oligonucleotides characterized by their use
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Abstract
The invention discloses development and application of KASP molecular markers of drought-tolerant genes of corn, belonging to the technical field of biology. Detecting the genotype of the corn to be detected, and identifying or assisting in identifying the drought tolerance of the corn according to the genotype of the corn to be detected; the genotype is the genotype of KASP _ RSP locus in the corn genome; the KASP _ RSP site is a SNP site in the maize genome. The invention provides development and application of a KASP marker tightly linked with a corn drought-tolerant gene RSP41, wherein the molecular marker is the KASP marker KASP _ RSP tightly linked with a corn drought-tolerant gene RSP 41. The marker is developed based on KASP technology, and can detect 73851206 th base of chromosome 5 of corn genome in high throughput. The invention applies KASP technology to carry out genotype identification on corn drought-enduring gene RSP41, has the advantages of simple and convenient operation, low cost, short detection period, stable marking, environmental protection and the like, can accurately detect the corn drought-enduring gene RSP41, and has important significance for promoting corn drought-enduring breeding.
Description
Technical Field
The invention relates to development and application of KASP molecular markers of drought-tolerant genes of corn, belonging to the technical field of biology.
Background
Corn is the most important grain crop and economic crop in China, and is also an important energy source and industrial raw material. According to the data of the national statistical bureau, the seeding area of the corn in 2019 in China reaches 41284.06 kilo hectares, and the yield reaches 26077.89 ten thousand tons. Northwest, northeast and other areas are main corn producing areas in China, but most areas are dry lands, and the corn yield is seriously damaged due to large water evaporation amount and quick loss. Drought is one of the major factors affecting the grain yield in China, and the cultivation of drought-tolerant corn varieties is the most economic and effective way for solving the drought problem.
Until now, some drought-enduring genes such as the nced gene, rab28 gene, dhn1 gene, rsp41 gene, etc. have been reported. Meanwhile, a molecular Marker linked with the gene is also developed, and drought tolerance improvement breeding of the corn is carried out by a Marker-assisted Selection (MAS) technology, so that a plurality of defects in the traditional breeding are overcome, and the method becomes an effective way for solving the problem that drought tolerance variety breeding is difficult.
However, although the linked markers of the drought-tolerant related genes of maize can be used for molecular marker-assisted selection, most of the markers are CAPS markers, SSR markers, InDel markers or enzyme digestion markers, the detection efficiency is low, and meanwhile, aerosol can be generated to pollute the environment, so that the method is not suitable for a high-throughput molecular detection platform. Therefore, the development of low-cost molecular markers capable of high-throughput detection is an urgent need to promote the identification of drought-tolerant genes of corn, increase the accuracy of drought-tolerant breeding and improve the breeding efficiency.
KASP (competitive Allele-Specific PCR) can achieve the genotyping effect by specifically identifying the gene locus through a fluorescent probe, and can quickly detect the SNP locus. Compared with molecular markers such as SSR, RFLP, InDel and the like, the KASP marker has the characteristics of high detection speed, low cost, easiness in large-scale application and the like, does not need to be typed according to the size of a DNA fragment, can get rid of the detection method of the traditional gel electrophoresis, which has relatively complicated steps, low throughput and higher price, and is more suitable for a high-throughput molecular detection platform which is rapidly developed at the present stage. Therefore, the development of the corn drought-tolerant gene KASP molecular marker which is low in cost and suitable for a high-throughput molecular detection platform has important significance for popularizing the application of a molecular marker technology and improving the drought-tolerant breeding efficiency and the breeding level of corn in China.
Disclosure of Invention
The present invention is implemented by the following technical solutions in view of the technical problems mentioned in the background art:
the development method of KASP molecular marker of drought-enduring gene of corn comprises detecting the genotype of corn to be detected, and identifying or assisting in identifying drought tolerance of corn according to the genotype of corn to be detected; the genotype is the genotype of KASP _ RSP locus in the corn genome; the KASP _ RSP site is an SNP site in a maize genome and is located at 73851206 th site of maize chromosome 5, the SNP site is C or T in nucleotide type and is the 51 st nucleotide of SEQ ID No. 4;
the corn to be detected with the genotype of the KASP-RSP locus as the CC genotype is drought-tolerant or candidate drought-tolerant corn; the corn to be detected with the genotype of the KASP-RSP locus as the TT genotype or the CT genotype is drought-intolerant or is selected to be drought-intolerant; wherein said CC genotype represents the homozygous type of the nucleotide species of said KASP _ RSP locus in the maize genome being C; the TT genotype represents that the nucleotide type of the KASP _ RSP site in the maize genome is homozygote of T; the CT genotype represents that the nucleotide type of the KASP _ RSP site in the maize genome is a hybrid type of C and T.
The application of KASP molecular marker of drought-enduring gene of corn, which comprises the following aspects:
1) the application of the substance of the polymorphism or genotype of the KASP _ RSP locus in the identification or the auxiliary identification of the drought tolerance of the corn;
2) the application of the substance of the polymorphism or genotype of the KASP _ RSP locus in the preparation of products for identifying or assisting in identifying drought tolerance of corn;
3) and the application of the substance of the polymorphism or genotype of the KASP _ RSP locus in the auxiliary breeding of the corn or the preparation of the auxiliary breeding product of the corn.
The corn breeding method provided by the method for developing KASP molecular markers of drought-enduring genes of corn comprises the following steps:
comprising selecting as a parent maize having as a parent a genotype CC for said KASP _ RSP site of claim 1, said CC genotype representing a homozygous type for C for the nucleotide species of said KASP _ RSP site in the maize genome; the corn breeding can be the cultivation of drought-tolerant corn.
The product produced by the method for developing KASP molecular marker of drought-enduring gene of corn mainly comprises the following products,
C1) detecting products of SNP polymorphism or genotype related to drought tolerance of corn;
C2) identifying or assisting in identifying drought tolerant products of maize;
C3) the product is used for auxiliary breeding of corn.
As preferred examples, the substance of the KASP _ RSP site polymorphism or genotype is D1), D2), or D3) as follows:
D1) the means for detecting the polymorphism or genotype of the KASP _ RSP site of claim 1 comprises PCR primers for amplifying a maize genomic DNA fragment including said KASP _ RSP site;
D2) the substance for detecting the polymorphism or genotype of KASP _ RSP site according to claim 1 is PCR reagent containing the PCR primer;
D3) a kit containing the PCR primer described in D1) or the PCR reagent described in D2).
As a preferable example, the PCR primer is a primer set consisting of a single-stranded DNA whose nucleotide sequence is the 22 nd to 44 th positions of SEQ ID No.1 of the sequence Listing, a single-stranded DNA whose nucleotide sequence is the 22 nd to 44 th positions of SEQ ID No.2 of the sequence Listing, and a single-stranded DNA whose nucleotide sequence is SEQ ID No.3 of the sequence Listing.
As a preferable example, the PCR primer is a primer set of a single-stranded DNA represented by SEQ ID No.1 of the sequence Listing, a single-stranded DNA represented by SEQ ID No.2 of the sequence Listing, and a single-stranded DNA represented by SEQ ID No.3 of the sequence Listing.
The invention has the beneficial effects that: the invention provides development and application of a KASP marker tightly linked with a corn drought-tolerant gene RSP41, wherein the molecular marker is the KASP marker KASP _ RSP tightly linked with a corn drought-tolerant gene RSP 41. The marker is developed based on KASP technology, and can detect 73851206 th base of chromosome 5 of corn genome in high throughput. The invention applies KASP technology to carry out genotype identification on corn drought-enduring gene RSP41, has the advantages of simple and convenient operation, low cost, short detection period, stable marking, environmental protection and the like, can accurately detect the corn drought-enduring gene RSP41, and has important significance for promoting corn drought-enduring breeding.
Drawings
FIG. 1 is a typing chart of 8 test maize variety materials detected by using the molecular marker KASP _ RSP in example 1, wherein FAM indicates genotype TT, VIC indicates genotype CC, and H indicates genotype CT;
FIG. 2 is a graph showing the genotyping of 48F 2 test populations tested using the molecular marker KASP _ RSP in example 2, in which FAM indicates genotype TT, VIC indicates genotype CC, and H indicates genotype CT.
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The examples provided below serve as a guide for further modifications by a person skilled in the art and do not constitute a limitation of the invention in any way.
The experimental procedures in the following examples, unless otherwise indicated, are conventional and are carried out according to the techniques or conditions described in the literature in the field or according to the instructions of the products. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The corn varieties used in the examples described below are all commercially available.
The invention discloses a SNP site related to drought tolerance of maize (Liu S, Hao Z, Weng J, et al. identification of two functional markers associated with moisture resistance in mail [ J ]. Molecular Breeding,2015,35(1):1-10.), which is anchored at 73851206 th position of maize chromosome 5 (https:// www.ncbi.nlm.nih.gov/association/GCF _902167145.1/, genome version Zm-B73-REFERENCE-NAM-5.0), and the flanking sequence of the SNP is shown as SEQ ID No. 4. KASP _ RSP is located at position 51 of SEQ ID No.4, where the nucleotide sequence is C or T.
The following CC genotypes represent homozygous for the nucleotide sequence of KASP _ RSP as C; the TT genotype represents that the nucleotide sequence of KASP-RSP is homozygote of T; the CT genotype indicates that the nucleotide type of KASP _ RSP is a hybrid of C and T.
Example 1
Development of KASP marker tightly linked with corn drought-tolerance related gene RSP41
1. Designing a primer: the flanking sequences of 50bp each from the left and right are extracted from the KASP _ RSP site, and 3 sets of KASP primers are preferably designed using Primer3.0 software. After detection using the ArrayTape platform from Douglas Scientific, 1 set of KASP primers with good polymorphism was selected for subsequent validation.
The KASP primers for detecting KASP _ RSP are specifically as follows:
primer X: 5'-gaaggtcggagtcaacggattGCATAGTAGACAAGTTTCCTGCC-3' (SEQ ID No.1, lower case letter portion is specific fluorescent tag sequence VIC);
primer Y: 5'-gaaggtgaccaagttcatgctGCATAGTAGACAAGTTTCCTGCT-3' (SEQ ID No.2, lower case letter portion is specific fluorescent tag sequence FAM);
Primer R：5’-TGTGCAAGTTGGTAATGTTAGCC-3’(SEQ ID No.3)。
DNA extraction: the genome DNA is extracted from the corn leaves by the conventional CTAB method.
KASP reaction test
KASP marker amplification and reaction system:
(1) and (3) detecting by using a fluorescent quantitative PCR instrument AB-Q6 Flex:
the detection reaction system of the 5-mu-L PCR fluorescence quantitative instrument comprises: 50ng of genomic DNA, and 0.07. mu.L of a Primer mixture (preferably, the ratio of the Primer mixture: 12. mu.L each of forward Primer X and Primer Y100 pmol. L-1, reverse Primer R100 pmol. L-130. mu.L, ddH2O 46. mu.L, and the same detection object can be achieved by using other reasonable ratios of the Primer mixture), 2.5. mu.L of LGC company 2 XKASP Mix (Low Rox). Editing a sample table, executing a running program and storing data according to an AB-Q6 instrument operation manual of the fluorescent quantitative PCR instrument.
The above reaction system is the preferred reaction system of AB-Q6 Flex, and other reasonable reaction systems can also achieve the same detection purpose.
(2) The selection of the ArrayTape platform from Douglas Scientific
1.6 u L PCR ArrayTape platform detection reaction system includes: genomic DNA 50 ng/. mu.L 0.8. mu.L, Primer Mix 0.03. mu.L (preferred Primer Mix ratios: 12. mu.L for each of forward Primer X and Primer Y100 pmol. L-1, reverse Primer R100 pmol. L-130. mu.L, ddH2O 46. mu.L, the same detection object can be achieved by using other reasonable Primer Mix ratios), LGC 2 XKASP Mix (Std Rox) 0.8. mu.L. According to an ArrayTape platform instrument operation manual, a sample table is compiled, a program is operated, and data are read.
The above reaction system is the preferable reaction system of the ArrayTape platform of Douglas Scientific company, and other reasonable reaction systems can achieve the same detection purpose.
Note: the above is a recommended detection method, and other detection methods capable of achieving the same detection purpose can also be applied to the molecular marker-assisted breeding process of the marker.
Wherein, the 2 XKASP Mix consists of a fluorescent probe A, a fluorescent probe B, a quenching probe A, a quenching probe B, high fidelity Taq enzyme, dNTP, Mg2+, and the like. The nucleotide sequence of the fluorescent probe A is as follows: 5'-GAAGGTCGGAGTCAACGGATT-3', connecting a VIC fluorescent group at the 5 ' end; the nucleotide sequence of the fluorescent probe B is as follows: 5'-GAAGGTGACCAAGTTCATGCT-3', wherein the 5 ' end is connected with a FAM fluorescent group; the nucleotide sequence of the quenching probe A is as follows: 5'-AATCCGTTGACTCCGACCTTC-3', wherein the 3 ' end is connected with a quenching group BHQ; the nucleotide sequence of the quenching probe B is as follows: 5'-AGCATGAACTTGGTCACCTTC-3', wherein the 3 ' end is connected with a quenching group BHQ.
And (3) amplification procedure: pre-denaturation at 95 ℃ for 10min for 1 cycle; denaturation at 95 ℃ for 20s, annealing at 55-62 ℃ (preferably 55 ℃) for 60s, setting 40 cycles.
The experiment was carried out while setting a blank control (NTC) without adding template DNA to the reaction system, and 1 or more blank controls were set for each plate.
Analyzing the scanning data, and then determining the genotype of the KASP _ RSP locus in the corn genome to be detected (namely, detecting whether the 73851206 th base of the No. 5 chromosome of the corn genome is C or T), wherein if the fluorescence signal data of the amplification product of the corn to be detected is analyzed to be close to an X axis (VIC signal) by Douglas genotyping software, the genotype of the KASP _ RSP locus in the corn genome to be detected is CC homozygous (namely, the 73851206 th base of the No. 5 chromosome of the corn genome is C homozygous); if the fluorescence signal data of the amplified product of the corn to be detected is analyzed to be close to a Y axis (FAM signal) by Douglas genotyping software, the genotype of the KASP _ RSP site in the corn genome to be detected is TT homozygosis (namely, the 73851206 th base of the No. 5 chromosome of the corn genome is T homozygosis); if the fluorescence signal data of the amplified product of the corn to be detected is positioned between an X axis and a Y axis (VIC and FAM signals) through Douglas genotyping software analysis, the genotype of the KASP _ RSP site in the corn genome to be detected is a CT heterozygous type (namely, the 73851206 th base of the No. 5 chromosome of the corn genome is the CT heterozygous type). The samples shown black in the lower left corner are blank controls.
5. Label typing data analysis
To verify the reliability of the KASP _ RSP marker, 8 corn materials were field identified to obtain field drought tolerance data for the materials. The genotype of the material was detected using the ArrayTape platform from Douglas Scientific, and the detection method, reaction system and amplification procedure were carried out according to the preferred protocol described above.
The amplification results showed that the KASP RSP marker was able to obtain stable PCR products in 8 material fractions and both C and T allelic sites could be detected (fig. 1). One drought tolerant corn material is CC genotype, and seven drought intolerant corn materials are TT genotype. Therefore, the KASP _ RSP marker can be used for breeding different drought-enduring corns.
Therefore, the KASP _ RSP marker can be used for molecular marker-assisted breeding of the drought-tolerant related gene RSP41 of the corn.
TABLE 1.8 drought tolerant phenotypic and genotypic information for test corn material
| Name of Material | Drought tolerance | Genotype(s) | Name of Material | Drought tolerance | Genotype(s) |
| Liao 173 | M | TT | Zheng 30 | M | TT |
| Chang-3 | M | TT | Yellow morning four | M | TT |
| Zheng 22 | M | TT | B73 | R | CC |
| Zheng 28 | R | CC | Dan 340 | M | TT |
Note: CC, genotype is homozygote of C; TT, genotype is homozygous for T; CT, wherein the genotype is a heterozygous type of C and T; ' indicates no detection signal; HR means high drought tolerance; r represents drought tolerance; m represents drought intolerance; HM means high intolerance to drought.
Example 2
Application of KASP marker closely linked with corn drought-tolerant related gene RSP41 in molecular marker-assisted selection of corn drought-tolerant plants
To test the utility of the KASP _ RSP marker of the present invention, the corn drought-tolerant material B73 (as male parent) described in Table 1 was hybridized with the corn drought-intolerant material Chang-3 (as female parent) to prepare F2 isolate, the F2 isolate was subjected to KASP marker test and drought-tolerant phenotype statistics (see FIG. 2, Table 2 for test results), and the marker test and drought-tolerant identification were performed according to the method described in example 1. By statistical analysis of drought tolerance and genotype of segregating populations, only 4 material phenotypes did not match genotype, with phenotype-genotype identity P of 91.67% (identity P: number of plants with phenotype matching genotype/total number of plants 100%). The above results indicate the marking
KASP _ RSP has higher practicability in screening drought-enduring plants of corns.
TABLE 2 phenotypic and genotypic information for isolated populations of maize
| Material numbering | Drought tolerance | Genotype(s) | Material numbering | Drought tolerance | Genotype(s) |
| 733_F2_01 | M | CT | 733_F2_25 | HM | CT |
| 733_F2_02 | M | CT | 733_F2_26 | M | CT |
| 733_F2_03 | R | CC | 733_F2_27 | M | CT |
| 733_F2_04 | M | CT | 733_F2_28 | M | TT |
| 733_F2_05 | HR | CC | 733_F2_29 | M | TT |
| 733_F2_06 | M | CT | 733_F2_30 | M | CT |
| 733_F2_07 | M | CT | 733_F2_31 | M | CT |
| 733_F2_08 | R | CT | 733_F2_32 | M | CT |
| 733_F2_09 | M | CT | 733_F2_33 | M | TT |
| 733_F2_10 | M | CT | 733_F2_34 | M | CT |
| 733_F2_11 | HM | CT | 733_F2_35 | R | CC |
| 733_F2_12 | M | CT | 733_F2_36 | M | CC |
| 733_F2_13 | M | CT | 733_F2_37 | M | TT |
| 733_F2_14 | R | CC | 733_F2_38 | M | TT |
| 733_F2_15 | M | CT | 733_F2_39 | M | TT |
| 733_F2_16 | M | CT | 733_F2_40 | M | CT |
| 733_F2_17 | R | CC | 733_F2_41 | M | TT |
| 733_F2_18 | M | TT | 733_F2_42 | HM | TT |
| 733_F2_19 | M | CT | 733_F2_43 | R | CC |
| 733_F2_20 | M | CT | 733_F2_44 | R | CC |
| 733_F2_21 | M | CC | 733_F2_45 | HR | CC |
| 733_F2_22 | R | CC | 733_F2_46 | M | CT |
| 733_F2_23 | M | TT | 733_F2_47 | M | CT |
| 733_F2_24 | R | TT | 733_F2_48 | M | CT |
Note: CC, genotype is homozygote of C; TT, genotype is homozygous for T; CT, wherein the genotype is a heterozygous type of C and T; ' indicates no detection signal; HR means high drought tolerance; r represents drought tolerance; m represents drought intolerance; HM means high intolerance to drought.
The invention has been described in detail with respect to the foregoing and it will be apparent to those skilled in the art that it is possible to practice the invention in a wide range of equivalent parameters, concentrations and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.
Sequence listing
<110> department of agricultural sciences of Linyun harbor City
<120> development and application of KASP molecular marker of corn drought-resistant gene
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<213> corn (Zea mays)
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Claims (7)
1. The method for developing KASP molecular markers of drought-tolerant genes of corn is characterized by comprising the following steps: detecting the genotype of the corn to be detected, and identifying or assisting in identifying the drought tolerance of the corn according to the genotype of the corn to be detected; the genotype is the genotype of KASP _ RSP locus in the corn genome; the KASP _ RSP site is an SNP site in a maize genome and is located at 73851206 th site of maize chromosome 5, the SNP site is C or T in nucleotide type and is the 51 st nucleotide of SEQ ID No. 4;
the corn to be detected with the genotype of the KASP-RSP locus as the CC genotype is drought-tolerant or candidate drought-tolerant corn; the corn to be detected with the genotype of the KASP-RSP locus as the TT genotype or the CT genotype is drought-intolerant or is selected to be drought-intolerant; wherein said CC genotype represents the homozygous type of the nucleotide species of said KASP _ RSP locus in the maize genome being C; the TT genotype represents that the nucleotide type of the KASP _ RSP site in the maize genome is homozygote of T; the CT genotype represents that the nucleotide type of the KASP _ RSP site in the maize genome is a hybrid type of C and T.
2. The use of KASP molecular markers for drought tolerance genes in maize as claimed in claim 1, wherein said use comprises the following aspects:
1) the application of the substance of the polymorphism or genotype of the KASP _ RSP locus in the identification or the auxiliary identification of the drought tolerance of the corn;
2) the application of the substance of the polymorphism or genotype of the KASP _ RSP locus in the preparation of products for identifying or assisting in identifying drought tolerance of corn;
3) and the application of the substance of the polymorphism or genotype of the KASP _ RSP locus in the auxiliary breeding of the corn or the preparation of the auxiliary breeding product of the corn.
3. The method of claim 1 for breeding maize, comprising the steps of:
comprising selecting as a parent maize having as a parent a genotype CC for said KASP _ RSP site of claim 1, said CC genotype representing a homozygous type for C for the nucleotide species of said KASP _ RSP site in the maize genome; the corn breeding can be the cultivation of drought-tolerant corn.
4. The product produced by the method for developing KASP molecular markers of drought tolerance genes in corn according to claim 1, which is characterized by mainly comprising the following products,
C1) detecting products of SNP polymorphism or genotype related to drought tolerance of corn;
C2) identifying or assisting in identifying drought tolerant products of maize;
C3) the product is used for auxiliary breeding of corn.
5. The product produced by the method for developing KASP molecular markers of maize drought tolerance genes as claimed in claim 2 or the method for developing KASP molecular markers of maize drought tolerance genes as claimed in claim 4, wherein: the substances of KASP _ RSP site polymorphism or genotype are D1), D2) or D3) as follows:
D1) the means for detecting the polymorphism or genotype of the KASP _ RSP site of claim 1 comprises PCR primers for amplifying a maize genomic DNA fragment including said KASP _ RSP site;
D2) the substance for detecting the polymorphism or genotype of KASP _ RSP site according to claim 1 is PCR reagent containing the PCR primer;
D3) a kit containing the PCR primer described in D1) or the PCR reagent described in D2).
6. Use or product according to claim 5, characterized in that: the PCR primer is a primer group consisting of single-stranded DNA with a nucleotide sequence of 22 th to 44 th positions of SEQ ID No.1 in a sequence table, single-stranded DNA with a nucleotide sequence of 22 nd to 44 th positions of SEQ ID No.2 in the sequence table and single-stranded DNA with a nucleotide sequence of SEQ ID No.3 in the sequence table.
7. Use or product according to claim 5 or 6, characterized in that: the PCR primer is a primer group consisting of a single-stranded DNA shown by SEQ ID No.1 in a sequence table, a single-stranded DNA shown by SEQ ID No.2 in the sequence table and a single-stranded DNA shown by SEQ ID No.3 in the sequence table.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115948591A (en) * | 2022-07-19 | 2023-04-11 | 河南省农业科学院粮食作物研究所 | Haplotype ZmC10.HapDR related to identifying drought tolerance of corn in seedling stage and application thereof |
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2021
- 2021-12-02 CN CN202111473100.2A patent/CN114150082A/en active Pending
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2022
- 2022-06-30 CN CN202210760857.8A patent/CN115505648A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115948591A (en) * | 2022-07-19 | 2023-04-11 | 河南省农业科学院粮食作物研究所 | Haplotype ZmC10.HapDR related to identifying drought tolerance of corn in seedling stage and application thereof |
| CN115948591B (en) * | 2022-07-19 | 2023-08-15 | 河南省农业科学院粮食作物研究所 | Identification of corn seedling drought tolerance related monomer ZmC10.HapDR and application thereof |
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