CN107815507A - For detecting SNP marker and the application of rice brown planthopper resistant Bph14 genes - Google Patents
For detecting SNP marker and the application of rice brown planthopper resistant Bph14 genes Download PDFInfo
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- CN107815507A CN107815507A CN201711262445.7A CN201711262445A CN107815507A CN 107815507 A CN107815507 A CN 107815507A CN 201711262445 A CN201711262445 A CN 201711262445A CN 107815507 A CN107815507 A CN 107815507A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
The present invention is provided to detect brown planthopper resistant gene in rice Bph14 SNP marker and application.The invention discloses the SNP marker K_030513 isolated with brown planthopper resistant gene in rice Bph14 and expanding effect is good, (MSU7.0) base at mark detection No. 3 chromosome 35692898 of rice, polymorphism is C/G, based on the mark K_030513 primer sequences of KASP technological development as shown in SEQ ID No.1 3.The SNP marker of the present invention can be used for the site that detection Bph14 gene locis are high specific, can convenient and efficient be used for identifying in rice varieties whether contain Bph14 genes.The application process of SNP marker provided by the invention is accurately and reliably, easy to operate, identification and assisted selection suitable for Bph14 genes.
Description
Technical field
The present invention relates to technical field of molecular biology, and in particular to brown planthopper resistant gene in rice Bph14 SNP molecule marks
The method remembered and detect the molecular labeling.
Background technology
Rice is the important cereal crops in China.Brown paddy plant hopper is a kind of rice monophagy insect, sucks bast by lancet
Portion's juice is caused harm rice strain, cause paddy growth slowly, tiller delay, empty flat grain increase.Brown paddy plant hopper or grass-like bushy stunt virus and
The communication media of some Rice Virus such as tingia dwarf virus, have a strong impact on the production and safety of rice.At present, to brown paddy plant hopper
Preventing and treating depends on chemical prevention, not only adds the drug resistance of production cost and insect, and pollutes environment, therefore, profit
It is considered as to prevent and treat the effective way of its harm to cultivate brown planthopper resistant kind with host resistance.
It is up to the present, identified and report at least 34 brown planthopper resistant gene sites, wherein dominant gene 19,
Recessive gene 15, main effect brown planthopper resistant gene after positioning there are 4 resistant genes to be cloned up to 28, be Bph3 respectively
(Liu et al.2014), Bph14 (Du et al.2009), Bph9 (Zhao et al.2016) and Bph26 (Ji et
al.2016).Huang etc. identifies two new dominant resistance bases from the gene transgression system B5 with oryza officinalis background
Cause, and RFLP technologies are used, one of them is named as to Bph14 (original name Qbp1) assignment of genes gene mapping using group's segregation analysis
Between the G1318 and R1925 of the 3rd chromosome, 2 molecular labelings are at a distance of 3.2cM.Bph14 be in rice first be cloned
Anti insect gene, cDNA total length 9576bp include 3 extrons, and one albumen being made up of 1323 amino acid of coding produces
Thing, product are typical CC-NB-LRR ill-resistant proteins structure.The gene while antibiont type I, II, III, without carrying
In Bph14 rice varieties platform No. 1 it is then sensitive to brown paddy plant hopper, brown paddy plant hopper, which connects, shows as that stalk is withered and yellow, and blade is wilted, fertility after worm
It is dead to reduce even whole strain.Wang Hui, 2016；National rice data center).
Due to pest-resistant phenotypic evaluation process very complicated, the breeding efficiency of rice brown planthopper resistant kind is limited, and is utilized
Conventional breeding means are often difficult to effectively polymerize different anti insect genes.Exploitation and anti insect gene close linkage isolate
Molecular labeling, it polymerize one or more using molecular marker assisted selection (Marker-assisted selection, MAS) technology
Individual target gene or QTL, so as to seed selection durable resistance kind, delay the degeneration time limit of pest-resistant cultivar and prevent brown paddy plant hopper neoformation
The generation of type.
Traditional rice breeding for pest resistance be by Resistance Identification to plant carry out Phenotypic Selection, expend the time length, and easily by
The limitation of environmental condition, qualification result easily cause error, and efficiency of selection is relatively low.It is simple using molecular marker assisted selection breeding
Effectively, breeding cost can be reduced, shortens the seed selection cycle, can also carry out autotelic multiple gene polymerization, improves breeding efficiency, band
Carry out huge economic results in society.The type mainly utilized in document report is that SSR and InDel is marked, and is deposited in breeding
It is relatively low in polymorphic rate, the less shortcoming of difference.The EB or Polyscrylamide used in detection process is easily polluted to environment, is right
Human body produces harm.Exploitation is with the brown planthopper resistant gene Bph14 specific molecular markers isolated and establishing efficient, environment-friendly
Coherent detection system, then to promoting application of the Bph14 genes in Hybrid breeding in commercial system significant.Base based on KASP
Because classifying method is to be recorded by computer and fluorescence signal caused by analyzing during PCR, realizes and mutational site is monitored.Inspection
It is high with phenotype uniformity to survey result；Detection process without electrophoresis, thoroughly prevented PCR primer Aerosol Pollution and EB to ring
Harm of the pollution, formaldehyde in border to human body.
The content of the invention
It is an object of the invention to provide a kind of SNP marker for being used to detect brown planthopper resistant gene in rice Bph14 and answer
With.
In order to realize the object of the invention, technical scheme is as follows：According to the information of gene, brown planthopper resistant gene
Bph14 genes are located at No. 3 chromosome 35693286-35699010 sections of rice, are respectively expanded to both sides centered on gene interval
Whether 50kb extracts SNP site, and have other SNP sites etc. to be selected according to PIC values and SNP site periphery 50bp.Meanwhile
Bph14 gene orders according to having cloned are compared with Nipponbare sequence, the SNP site picked out, and utilize
BatchPrimer3 carries out design of primers to it.For these SNP markers, to containing Bph14 genetic donor materials B5, China 2211
KASP reaction checkings are carried out with other multiple rice varieties without Bph14, picking out specific can distinguish resistant donor material
And the SNP marker K_030513 that expanding effect is good.The chain SNP of resistant gene selected is marked by 95 parts of natural population's materials
Remember that K_030513 carries out natural population's checking, it is rare bases to show resistance bases.Heredity has been carried out using 79 plants of RIL colonies
Location verification and phenotype checking.
The invention provides a kind of SNP marker K_030513 for detecting brown planthopper resistant gene in rice Bph14, alkali is detected
Base is located at No. 3 dyeing the 35692898th (MSU7.0 genomes version) of rice, and the site is located at Bph14 gene intervals upstream
At 388bp, the SNP marker polymorphism is C/G.
The above-mentioned SNP marker of the present invention is following specific primer sets,
(1) two specific primer：
PrimerX：5’-ATTGCAGCGTTATCGGGAG-3’；
PrimerY：5’-ATTGCAGCGTTATCGGGAC-3’；
(2) universal primers：
PrimerC：5’-TGCAGAGGATACTTGTGATTG-3’.
The invention provides the specific primer sets for detecting above-mentioned SNP marker, including：
(1) two specific primer：
PrimerX：5’-ATTGCAGCGTTATCGGGAG-3’；
PrimerY：5’-ATTGCAGCGTTATCGGGAC-3’；
(2) universal primers：
PrimerC：5’-TGCAGAGGATACTTGTGATTG-3’.
Reagent or kit containing above-mentioned specific primer sets belong to protection scope of the present invention.
The invention provides application of the above-mentioned specific primer sets in brown planthopper resistant gene in rice Bph14 is detected.
Described application, further comprises the steps：
(1) oryza sativa genomic dna to be measured is extracted；
(2) using oryza sativa genomic dna as template, KASP reaction detections are carried out using above-mentioned specific primer sets
(3) if only detecting base C corresponding to primer PrimerY, judge that rice to be measured is free of Bph14 genes；If only
The bases G corresponding to primer PrimerX is detected, then judges that rice to be measured contains the Bph14 genes of homozygosis；If it is detected simultaneously by
Base C and G, then judge the Bph14 genes that rice to be measured contains heterozygosis.
PCR conditions are in KASP detections in step (2)：94℃15min；94 DEG C of 20sec, 65-57 DEG C of 1min, each circulation
Annealing temperature reduces by 0.8 DEG C, totally 10 circulations；94 DEG C of 20sec, 57 DEG C of 1min, totally 26 circulations.
The invention provides the method for detection rice brown planthopper resistant Bph14 genotype.
The invention provides detection SNP marker K_030513 method.
The invention provides applications of the SNP marker K_030513 in auxiliary identifies rice Bph14 genes.
The invention provides SNP marker K_030513 or above-mentioned specific primer sets or contain the specific primer
Application of the kit of combination in Rice Germplasm Resources improvement.
The invention provides SNP marker K_030513 or above-mentioned specific primer sets or contain the specific primer
Application of the kit of combination in cultivating with brown planthopper resistant ability rice.
The present invention can be high-throughout using the mark of the single base difference design based on KASP reaction principles and anti-sense material
Bph14 resistant gene detections are carried out to rice material, each mark is made up of three primers, two specific primers in embodiment
Fluorescent linker sequence corresponding to KASP reaction reagents, a universal primer are connected respectively.If it is corresponding to only detect primer PrimerY
Base C, then judge that rice to be measured is free of Bph14 genes；If only detecting the bases G corresponding to primer PrimerX, judge
Rice to be measured contains the Bph14 genes of homozygosis；If being detected simultaneously by base C and G, judge that rice to be measured contains heterozygosis
Bph14 genes.Detected by two kinds of different fluorescence signals, realize that the genotype of the SNP site to each detection sample judges, energy
Enough overcome existing Morphological Identification method detection cycle length, need rich experiences and PCR, RAPD, PCR-RFLP, Southern
The shortcomings of detection method complex operations such as hybridization or not high or specific not strong or repeated bad detection sensitivity, thoroughly shut out
Pollution, formaldehyde harm to human body of the Aerosol Pollution and EB of PCR primer to environment absolutely, have it is easy, reliable, quick,
High specificity, high sensitivity, environment-friendly remarkable advantage.The application process of SNP marker provided by the invention accurately may be used
Lean on, easy to operate, identification and assisted selection suitable for Bph14 genes.
Brief description of the drawings
Fig. 1 is the application SNP marker K_030513 development process figure.
Fig. 2 is the application SNP marker K_030513 in natural population's genotyping result.
The genetic locus that Fig. 3 is the application SNP marker K_030513 is verified.
Embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It is it will be appreciated that following real
Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this area
Art personnel can carry out various modifications and replacement in the case of without departing substantially from spirit of the invention and spirit to the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
The exploitation of the SNP marker of the rice Bph14 genes of embodiment 1
According to the location information of gene, brown planthopper resistant gene Bph14 gene intervals are located at No. 3 chromosomes of rice
35693286-35699010 sections (MSU7.0).Respectively expand 50kb extraction SNP sites to both sides centered on gene interval, and
Whether there are other SNP sites etc. to be selected according to PIC values and SNP site periphery 50bp.Meanwhile according to having cloned
Bph14 gene orders are compared with Nipponbare sequence, the SNP site picked out, and it is drawn using BatchPrimer3
Thing designs.For these SNP markers, to containing Bph14 genetic donor material RH, IR56 and other 18 water without Bph14
Rice varieties carry out KASP reaction checkings, pick out and are isolated with resistant donor material and SNP marker K_ that expanding effect is good
030513.Above-mentioned SNP marker development process figure is shown in Fig. 1.
The primer of the present invention for being used to detect above-mentioned SNP marker is combined as：
(1) two specific primer：
PrimerX：5’-ATTGCAGCGTTATCGGGAG-3’；
PrimerY：5’-ATTGCAGCGTTATCGGGAC-3’；
(2) universal primers：
PrimerC：5’-TGCAGAGGATACTTGTGATTG-3’.
Two the end of specific primer 5 ' connects the specific FAM and HEX fluorescent linkers sequence of LGC company's KASP reaction reagents respectively
Row.The synthesis of primer commission Invitrogen companies.
Table 1 marks K_030513 primer information
| ID | MSU7.0 positions | Deng bit base type X | Deng bit base type Y | Favourable base type |
| K_030513 | chr03.35692898 | G | C | G |
KASP reactions are carried out to rice material genomic DNA to be measured.KASP reaction tests are in LGC SNPline Genotypings
Carried out on platform.20ng DNA samples are added in micro reaction plate, KASP reaction mixtures are added after drying, reaction system is shown in
Table 2.PCR amplifications are completed in water-bath thermal cycler, and Touchdown PCR reaction conditions are：94 DEG C of pre-degenerations 15 minutes；First
Amplified reaction is walked, 94 DEG C are denatured 20 seconds, and 65 DEG C~57 DEG C are annealed and extended 60 seconds, 10 circulations, each cycle annealing and extension
Temperature reduce by 0.8 DEG C；Second step amplified reaction, 94 DEG C are denatured 20 seconds, and 57 DEG C are annealed and extended 60 seconds, 26 circulations.Reaction
After the completion of using scanner Pherastar fluorescence data reading is carried out to KASP reaction products, the result of fluorescent scanning can be automatic
Change into figure.
The LGC SNPline Genotypings platforms used in the present invention are purchased from Britain LGC public affairs with its matched reagent consumptive material
Department.
The reaction system of table 2KASP detections
| Final concentration | Volume (ul) | |
| 100UM Primer C | 0.42UM | 0.0125 |
| 100UM Primer X | 0.17UM | 0.0050 |
| 100UM Primer Y | 0.17UM | 0.0050 |
| 2×KASP Master Mix | 1× | 1.4792 |
| Ultra-pure water | 1.4983 | |
| Cumulative volume | 3 |
To the rice varieties B5 containing Bph14 genes, China 2211, China extensive 1337 and other be free of with mark K_030513
Bph14 32 rice varieties carry out KASP reaction checkings, as a result such as table 3.The rice varieties of the gene containing Bph14 are in K_030513
Test site detects bases G, and except a material is without amplification, other 31 parts without Bph14 rice varieties, either other are anti-
Brown paddy plant hopper genetic donor or susceptible material detect base C in test site.
Table 3 marks K_030513 primary dcreening operation typing datas
The application of the SNP marker of the rice Bph14 genes of embodiment 2
High-throughout rice material can be carried out using KASP reactions for molecular labeling of the present invention design anti-brown
Plant hopper Bph14 genetic tests, the primer designed using embodiment 1 are combined.SNP marker K_030513 is carried out using 95 parts of materials
Natural population verifies.95 parts of materials include feeling worm control material, common hybridization rice and core rice breed.Mark is certainly
Right colony's genotyping result as shown in Fig. 2 in addition to association excellent 716 detects heterozygosis Bph14 genotype, other sense worm control materials,
Common hybridization rice and core rice breed are detected as no Brown Planthopper Resistance homozygosis Bph14 genotype.It can be seen that SNP marker
K_030513 detection Bph14 gene locis are the resistance locus of high specific, can convenient and efficient be used to identify in rice varieties
Whether Bph14 genes are contained.
Genetic locus and segregating population the phenotype checking of the SNP marker of embodiment 3
1st, genetic locus checking is marked
Using 79 plants of Bph14 RIL colonies and 11 in Parent in SNP marker and the present invention with polymorphism
Embodiment 1 develops obtained Bph14 specific markers K_030513 and carries out genetic locus checking, mark K_030513 of the invention
It is positioned to No. 3 chromosome 79.2cM position of rice (see Fig. 3).
2nd, phenotype checking is marked
Using B5 and Mh63 RIL colonies to carrying out hereditary table with gene Bph14 linked markers K_030513 in the present invention
Type is verified.Phenotypic evaluation entrusts Wuhan He Taiqing bio tech ltd, and 83.3% phenotype is in 12 strains containing Bph14
More than moderate resistance pest-resistant level, 83.3% phenotype is sense worm phenotype (table 4), phenotypic data and base in 12 strains without Bph14
Because type correspond to it is preferable, demonstrate again the present invention feasibility and accuracy.Identification and assisted Selection available for Bph14 genes
Breeding.
Table 4
Sequence table
<110>Hua Zhi rice biologicals Technology Co., Ltd.
<120>For detecting SNP molecular labelings and the application of rice brown planthopper resistant Bph14 genes
<130> KHP171115428.0
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 19
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 1
attgcagcgt tatcgggag 19
<210> 2
<211> 19
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 2
attgcagcgt tatcgggac 19
<210> 3
<211> 21
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 3
tgcagaggat acttgtgatt g 21
Claims (10)
- A kind of 1. SNP marker for being used to detect brown planthopper resistant gene in rice Bph14, it is characterised in that detection rice the 3rd The bit base of chromosome the 35692898th, the site are located at Bph14 gene interval 35693286-35699010 upstream 388bp, should SNP marker polymorphism is C/G.
- 2. SNP marker as claimed in claim 1, it is following specific primer sets,(1) two specific primer：PrimerX：5’-ATTGCAGCGTTATCGGGAG-3’；PrimerY：5’-ATTGCAGCGTTATCGGGAC-3’；(2) universal primers：PrimerC：5’-TGCAGAGGATACTTGTGATTG-3’.
- 3. the specific primer sets of 1 or 2 SNP markers are required for test right, it is characterised in that including：(1) two specific primer：PrimerX：5’-ATTGCAGCGTTATCGGGAG-3’；PrimerY：5’-ATTGCAGCGTTATCGGGAC-3’；(2) universal primers：PrimerC：5’-TGCAGAGGATACTTGTGATTG-3’.
- 4. reagent or kit containing specific primer sets described in claim 3.
- 5. application of the specific primer sets in brown planthopper resistant gene in rice Bph14 is detected described in claim 3.
- 6. application according to claim 5, it is characterised in that comprise the following steps：(1) oryza sativa genomic dna to be measured is extracted；(2) using oryza sativa genomic dna as template, KASP reaction inspections are carried out using the specific primer sets described in claim 3 Survey；(3) if only detecting base C corresponding to primer PrimerY, judge that rice to be measured is free of Bph14 genes；If only detect To the bases G corresponding to primer PrimerX, then judge that rice to be measured contains the Bph14 genes of homozygosis；If it is detected simultaneously by base C and G, then judge the Bph14 genes that rice to be measured contains heterozygosis.
- 7. the application according to claim 5 or 6, it is characterised in that PCR conditions are in KASP detections in step (2)：94℃ 15min；94 DEG C of 20sec, 65-57 DEG C of 1min, each cycle annealing temperature reduce by 0.8 DEG C, totally 10 circulations；94 DEG C of 20sec, 57 DEG C 1min, totally 26 circulations.
- 8. application of the SNP marker in auxiliary identifies rice Bph14 genes described in claim 1 or 2.
- 9. the specific primer sets or claim 4 described in SNP marker or claim 3 described in claim 1 or 2 Application of the described kit in Rice Germplasm Resources improvement.
- 10. the specific primer sets or claim described in SNP marker or claim 3 described in claim 1 or 2 Application of the kit in cultivating with Brown Planthopper Resistance rice described in 4.
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| CN108707674A (en) * | 2018-05-30 | 2018-10-26 | 中山大学 | The method that brown paddy plant hopper group confrontation worm rice varieties IR36 causes evil power level is predicted by single nucleotide polymorphism |
| CN109913576A (en) * | 2019-04-15 | 2019-06-21 | 武汉禾泰青生物科技有限公司 | It is a kind of for detecting the primer pair and its application of brown planthopper resistant gene in rice Bph14 |
| CN112458198A (en) * | 2020-12-17 | 2021-03-09 | 华智生物技术有限公司 | Auxiliary breeding molecular marker of brown planthopper resistant gene Bph27 and application thereof |
| CN112725519A (en) * | 2021-03-01 | 2021-04-30 | 广西壮族自治区农业科学院 | PARMS marker based on brown planthopper resistance gene Bph14 of rice and application |
| CN114271158A (en) * | 2021-12-30 | 2022-04-05 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
| CN114410623A (en) * | 2022-02-17 | 2022-04-29 | 武汉大学深圳研究院 | SNP molecular marker and application thereof in detection of brown planthopper resistant gene Bph44(t) of rice |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707674A (en) * | 2018-05-30 | 2018-10-26 | 中山大学 | The method that brown paddy plant hopper group confrontation worm rice varieties IR36 causes evil power level is predicted by single nucleotide polymorphism |
| CN108707674B (en) * | 2018-05-30 | 2021-08-03 | 中山大学 | Method for predicting pest-causing capacity level of brown planthopper population to resist insect rice variety IR36 through single nucleotide polymorphism |
| CN109913576A (en) * | 2019-04-15 | 2019-06-21 | 武汉禾泰青生物科技有限公司 | It is a kind of for detecting the primer pair and its application of brown planthopper resistant gene in rice Bph14 |
| CN112458198A (en) * | 2020-12-17 | 2021-03-09 | 华智生物技术有限公司 | Auxiliary breeding molecular marker of brown planthopper resistant gene Bph27 and application thereof |
| CN112725519A (en) * | 2021-03-01 | 2021-04-30 | 广西壮族自治区农业科学院 | PARMS marker based on brown planthopper resistance gene Bph14 of rice and application |
| CN114271158A (en) * | 2021-12-30 | 2022-04-05 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
| CN114271158B (en) * | 2021-12-30 | 2022-12-23 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
| CN114410623A (en) * | 2022-02-17 | 2022-04-29 | 武汉大学深圳研究院 | SNP molecular marker and application thereof in detection of brown planthopper resistant gene Bph44(t) of rice |
| CN114410623B (en) * | 2022-02-17 | 2024-04-23 | 武汉大学深圳研究院 | SNP molecular marker and application thereof in detection of brown planthopper resistant gene Bph44 (t) of rice |
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