CN107853447A - A kind of deer whip active peptide and preparation method - Google Patents
A kind of deer whip active peptide and preparation method Download PDFInfo
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- CN107853447A CN107853447A CN201711068428.XA CN201711068428A CN107853447A CN 107853447 A CN107853447 A CN 107853447A CN 201711068428 A CN201711068428 A CN 201711068428A CN 107853447 A CN107853447 A CN 107853447A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
- A23J1/002—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from animal waste materials
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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Abstract
The invention provides a kind of preparation method of deer whip active peptide.The preparation method is raw material using deer whip, prepares refined deer whip active peptide and full nutrition deer whip active peptide.Efficiently digested using combination of enzyme preparations, reduce energy and material consumption；And the creative nutritional ingredient that with the addition of traditional Chinese medicine raspberry and cloves, further enrich deer whip peptide in enzyme deactivation is extracted.Particular filter auxiliary combination is used in separation process, greatly enhances refining spearation ability；Low-temperature spray drying tower in finally using, thermal activity material is protected, and middle low-temperature spray drying tower is also to be applied first in peptide technique.In addition, the preparation method raw material of the present invention is easy to get, simple production process, production cost is greatly reduced.And mass content >=80% of the refined deer whip active peptide prepared, mass content >=30% of full nutrition deer whip active peptide.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of deer whip active peptide and preparation method.
Background technology
Deer whip, Chinese medicine name, also known as deer kidney, deer punching.For animal in deer family sika deer or the external genital organs of red deer male.Deer whip is made
With a lot, can invigorate blood circulation, lactagogue.It is sweet, into warm-natured.Energy tonifying kidney and strengthening yang, strengthening the essence.It can be used for nourishing and strengthening vital, excited male sexual
Energy；For sex dysfunction, deer whip, which is steep in wine, is made deer whip tincture, deer whip treatment old man's many nights many urine nights urine trouble part.Deer
Whip can steep in wine, and make herbal cuisine, or cook, or endure cream, or enter ball, dissipate.Deer whip is containing protein, fat, font steroid hormone, dimension life
Plain C, A and inorganic calcium, phosphorus, iron etc., there is a kidney-replenishing, the work(of benefiting essence-blood, for straining, soreness of waist and knee joint finnitus due to the kidney deficiency, impotence, palace cold
Infertile, sex dysfunction, the impotence as caused by the deficiency of yang, premature ejaculation, and lumbocrural pain, have to woman infertility and postpartum agalorrhea aobvious
Curative effect.Deer whip is in addition to medicinal, and carnivorous first-class raw material, available for steeping in wine or with chicken, duck, goose, fish Baoshang or making
High-grade dishes.
With the development of science and technology, the application of protein engineering, biologically active peptide and its egg are produced using zymolysis technique
The research and product development of white hydrolysate have become focus and main flow, but the preparation method for producing deer whip peptide in industry does not have also
Have.
The content of the invention
An object of the present disclosure is to provide a kind of deer whip active peptide.
Another object of the present invention is to provide the preparation method of above-mentioned active peptide.
A kind of preparation method of deer whip active peptide, comprises the following steps：
A, deer whip is pre-processed, carries out ultramicro grinding, crossed 200~600 mesh, add water to mix, adjusted at 8~9 micro- alkalization of pH
Reason, soak 18~24h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, weak acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, from
The heart, collects precipitation, and precipitation is deer whip albumen；
C, precipitation plus water are redissolved, heating so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 5~10, adds special enzyme preparation, enzymolysis；
E, after enzymolysis terminates, specific Chinese traditional medicine composition is added, extraction enzyme deactivation obtains thick enzymolysis liquid after terminating；
F, deer whip active peptide powder is made in thick enzymolysis liquid.
The preparation method of deer whip active peptide described above, it is characterised in that F-step also includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, then spray drying obtains refined deer whip active peptide powder.
The preparation method of deer whip active peptide described above, it is characterised in that F-step also includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition deer whip active peptide
Powder.
The preparation method of deer whip active peptide described above, it is characterised in that in described step A, carrying out ultramicro grinding can
To improve enzymatic hydrolyzation, material composition is made full use of.
The preparation method of deer whip active peptide described above, it is characterised in that in described step B, the weak acid be acetic acid,
One or more in lactic acid, citric acid, 8~12h of time.Using weak acid precipitating proteins, strong acid is reduced to proteinaceous nutrient
The destruction of material, be advantageous to optimize the nutrition composition of active peptide.
The preparation method of deer whip active peptide described above, it is characterised in that in described step C, heating-up temperature is 75~
100 degree, 15~30 minutes time.Using the method for thermal denaturation, it can be more favorable for digesting, enzymolysis is more abundant.Thermal change simultaneously
Property after carry out high-pressure homogeneous, further improve enzymolysis efficiency.
The preparation method of deer whip active peptide described above, it is characterised in that described step D, special enzyme preparation combination bag
Include flavor protease: trypsase: neutral proteinase is 1~2: 1~2: 1~2.Research finds the enzyme of its special enzyme preparation combination
Solve the enzymolysis efficiency of the long-range about single enzyme of efficiency.
The preparation method of deer whip active peptide described above, it is characterised in that characterized in that, described step D, digests bar
Part is：Hydrolysis temperature is 40~65 DEG C, and enzymolysis time is 1.5~8h.
The preparation method of deer whip active peptide described above, it is characterised in that in described step E, specific Chinese traditional medicine composition is：
Raspberry and cloves, addition are the 1~2% of dry deer whip powder solid masses.Activity can further be played by adding traditional Chinese medicine
The nutritive value of peptide.
The preparation method of deer whip active peptide described above, it is characterised in that in described step E.Extract the condition of enzyme deactivation
For：It is 90~125 DEG C to extract enzyme-removal temperature, and the time is 0.5~3h.
The preparation method of deer whip active peptide described above, it is characterised in that in described step F1, special filtration adjuvant group
It is combined into：Activated carbon, perlite, diatomite, its ratio are 6~7: 1~2: 2~3.Research finds its special filtration adjuvant combination
The filter efficiency of the long-range about single filtering agent of filter efficiency.
The preparation method of deer whip active peptide described above, it is characterised in that being done for middle cold nebulization used in being spray-dried
Dry tower, further retain thermal activity material, and middle low-temperature spray drying tower is to be applied first in peptide technique.
A kind of deer whip active peptide, it is characterised in that be made according to any of the above-described described preparation method, the refined deer whip
Mass content >=80% of active peptide, mass content >=30% of the full nutrition deer whip active peptide.
The preparation method of the deer whip active peptide of the present invention, specific Chinese traditional medicine composition raspberry and cloves first can be further
Lift the nutritional ingredient healthcare function of the deer whip active peptide prepared.Raspberry and cloves are as follows to the main function of body：
Raspberry：It is a kind of xylophyta of rose family rubus, is a kind of fruit, fruit taste is sour-sweet, plant
With delavay raspberry herb on limb.Red raspberry contains organic acid, carbohydrate and a small amount of vitamin C, benzoaric acid, β~sitosterol, red raspberry
Acid.Eugenol and aceteugenol can suppress rabbit and human platelet aggregation and the Whole Blood Aggregation of arachidonic acid-induction, and suppress
ATP discharges.It resists poly- mechanism to suppress the synthesis of TXB in blood platelet.Contain 17 kinds of amino in the fruit and blade of raspberry
Acid, wherein 7 kinds of essential amino acid, polysaccharide, flavones, etc. compound.It is edible to have warming kidney and enhancing yang effect.Scientific research personnel studies hair
It is existing, contain a large amount of polyphenoils in raspberry, and this kind of material is after entrance body, in the pars iotica retinae site concentration of eyeball
It is higher, effectively it can prevent retina from aoxidizing, and remove the free radical being had damage to retina.So raspberry can use
To protect or improve eyesight, and the senile illness in eye (such as macular degeneration illness) for the treatment of has remarkable effect.It is contained in raspberry
Flavonoid substances, except with anti-inflammation, reduce blood pressure and the effect such as antiallergy in addition to, their also bodies in terms of skin-protecting face nursing
Hand is out of the ordinary.This kind of material can improve skin blood circulation, enhancing capillary of skin elasticity, promote Skin Cell regeneration, from
And play the function of beautifying face and moistering lotion.Cloves：It is cloves acrid flavour, warm-natured, have the function that middle benefit gas drop is inverse, tonify the kidney and support yang.It or one
Taste warm stomach medicine well, to the stomachache as caused by cold-evil, vomiting, hiccup, stomachache, diarrhea and the cold dysmenorrhoea of women etc., has
The effect of good, it can also alleviate belly flatulence, strengthen digestion power, mitigate nausea and vomiting, especially pregnant woman and suffer from morning sickness symptom.Fourth
Perfume (or spice) contains caryophyllus oil, has inhibitory action for pathogenic fungus and staphylococcus, dysentery and Escherichia coli etc., can also make external application,
There is the effect of fine to ringworm of the body and tinea pedis.Anti-gastric-ulcer：Experimental Gastric Ulcer in Rats can be suppressed to be formed.Volatile clove oil and fourth
Fragrant phenol can dramatically increase stomach mucilage secretion, and acidity does not increase.Eugenol is probably the active component of antiulcer.Stomach invigorating：Can
Alleviate belly flatulence, strengthen digestion power, mitigate nausea and vomiting.It is excited to suppress intestines：It is spontaneous that its water decoction can suppress isolated rabbit intestines
Property shrink, and can be to the excitation to intestine in vitro such as anti-acetylcholine, histamine.Anti diar rhea：Its water extract and eugenol energy
Big enteral diarrhea caused by antagonism folium sennae or castor oil and enteric cavity hydrops.Eugenol and aceteugenol can suppress arachidonic
The rabbit of acid induction and human platelet aggregation and Whole Blood Aggregation, and suppress ATP releases.It resists poly- mechanism to suppress in blood platelet
TXB synthesis.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Embodiment
With reference to specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to
This.
Embodiments of the invention are described in detail below, but the present invention can be limited and covered according to claim
Multitude of different ways implement.
Embodiment 1
A kind of preparation method of deer whip active peptide, comprises the following steps：
A, deer whip is pre-processed, carries out ultramicro grinding, crossed 400 mesh, add water to mix, adjust 8~9 micro- basifications of pH, leaching
Steep 20h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, acetic acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, from
The heart, collects precipitation, and precipitation is deer whip albumen；
C, precipitation plus water are redissolved, is heated to 90 degree so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 7~8, adds special enzyme preparation (flavor protease: trypsase: neutral proteinase
For 1.5: 1.5: 1), enzymolysis, hydrolysis temperature is 50 DEG C, enzymolysis time 2h；
E, after enzymolysis terminates, specific Chinese traditional medicine composition (raspberry and cloves) is added, extraction enzyme deactivation is obtained after terminating and slightly digested
Liquid；
F, deer whip active peptide powder is made in thick enzymolysis liquid.
The preparation method of deer whip active peptide described above, it is characterised in that F-step also includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, is then spray-dried to obtain refined deer whip active peptide powder.
The preparation method of deer whip active peptide described above, it is characterised in that F-step also includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition deer whip active peptide
Powder.
In described step E, the addition of raspberry and cloves is the 1.5% of dry deer whip powder solid masses.
In described step E, enzyme-removal temperature is 90 DEG C, enzymolysis time 1h.
In described step F1, special filtration adjuvant is combined as：Activated carbon, perlite, diatomite, its ratio are 6.7:
1.5∶2。
Embodiment 2
A kind of preparation method of deer whip active peptide, comprises the following steps：
A, dry deer whip is pre-processed, carries out ultramicro grinding, crossed 400 mesh, add water to mix, adjust 8~9 micro- basifications of pH,
Soak 20h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, acetic acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, from
The heart, collects precipitation, and precipitation is deer whip albumen；
C, precipitation plus water are redissolved, is heated to 90 degree so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 7~8, adds special enzyme preparation (flavor protease: trypsase: neutral proteinase
For 1.4: 1.6: 1.2), enzymolysis, hydrolysis temperature is 50 DEG C, enzymolysis time 2h；
E, after enzymolysis terminates, specific Chinese traditional medicine composition is added, extraction enzyme deactivation obtains thick enzymolysis liquid after terminating；
F, deer whip active peptide powder is made in thick enzymolysis liquid.
The preparation method of deer whip active peptide described above, it is characterised in that F-step also includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, is then spray-dried to obtain refined deer whip active peptide powder.
The preparation method of deer whip active peptide described above, it is characterised in that F-step also includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition deer whip active peptide
Powder.
In described step E, the addition of raspberry and cloves is the 1.7% of dry deer whip powder solid masses.
In described step E, enzyme-removal temperature is 95 DEG C, enzymolysis time 1h.
In described step F1, special filtration adjuvant is combined as：Activated carbon, perlite, diatomite, its ratio are 6.7:
1.8∶2。
Embodiment 3
A kind of preparation method of deer whip active peptide, comprises the following steps：
A, dry deer whip is pre-processed, carries out ultramicro grinding, crossed 400 mesh, add water to mix, adjust 8~9 micro- basifications of pH,
Soak 22h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, acetic acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, from
The heart, collects precipitation, and precipitation is deer whip albumen；
C, precipitation plus water are redissolved, is heated to 95 degree so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 7~8, adds special enzyme preparation (flavor protease: trypsase: neutral proteinase
For 1.6: 1.6: 1.4), enzymolysis, hydrolysis temperature is 55 DEG C, enzymolysis time 1.5h；
E, after enzymolysis terminates, specific Chinese traditional medicine composition (raspberry and cloves) is added, extraction enzyme deactivation is obtained after terminating and slightly digested
Liquid；
F, deer whip active peptide powder is made in thick enzymolysis liquid.
The preparation method of deer whip active peptide described above, it is characterised in that F-step also includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, is then spray-dried to obtain refined deer whip active peptide powder.
The preparation method of deer whip active peptide described above, it is characterised in that F-step also includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition deer whip active peptide
Powder.
In described step E, the addition of raspberry and cloves is the 1.7% of dry deer whip powder solid masses.
In described step E, enzyme-removal temperature is 100 DEG C, enzymolysis time 0.5h
In described step F1, special filtration adjuvant is combined as：Activated carbon, perlite, diatomite, its ratio are 6.5:
1.5∶2.5。
Peptide content the results are shown in Table 1 in active peptide in measure embodiment 1~3.
The content assaying method of peptide is：Carried out according to the assay method of peptide content in the soy peptide powders of GB/T 22492.
Table 1
| Peptide content | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Refined deer whip activity peptide content % | 82.1 | 83.2 | 83.7 |
| Full nutrition deer whip activity peptide content % | 37.2 | 35.6 | 34.2 |
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (10)
1. a kind of preparation method of deer whip active peptide, it is characterised in that comprise the following steps：
A, deer whip is pre-processed, carries out ultramicro grinding, crossed 200~600 mesh, add water to mix, adjust 8~9 micro- basifications of pH,
Soak 18~24h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, weak acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, centrifugation, receipts
Precipitation is taken, precipitation is deer whip albumen；
C, precipitation plus water are redissolved, heating so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 5~10, adds special enzyme preparation, enzymolysis；
E, after enzymolysis terminates, specific Chinese traditional medicine composition is added, extraction enzyme deactivation obtains thick enzymolysis liquid after terminating；
F, deer whip active peptide powder is made in thick enzymolysis liquid.
2. the preparation method of deer whip active peptide as claimed in claim 1, it is characterised in that F-step includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, then spray drying obtains refined deer whip active peptide powder.
3. the preparation method of deer whip peptide living as claimed in claim 1, it is characterised in that F-step includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition deer whip active peptide powder.
4. the preparation method of deer whip active peptide as claimed in claim 1, it is characterised in that in described step B, the weak acid
For the one or more in acetic acid, lactic acid, citric acid, 8~12h of time.
5. the preparation method of deer whip active peptide as claimed in claim 1, it is characterised in that in described step C, heating-up temperature
For 75~100 degree, 15~30 minutes time.
6. the preparation method of deer whip active peptide as claimed in claim 1, it is characterised in that described step D, special enzyme preparation
Combination includes flavor protease: trypsase: neutral proteinase is 1~2: 1~2: 1~2.
7. the preparation method of deer whip active peptide as claimed in claim 1, it is characterised in that described step D, enzymatic hydrolysis condition
For：It is 40~65 DEG C to extract hydrolysis temperature, and the time is 1.5~8h.
8. the preparation method of deer whip active peptide as claimed in claim 1, it is characterised in that in described step E, specific Chinese medicine
It is combined as：Raspberry and cloves, addition are the 1~2% of dry deer whip powder solid masses.Extraction enzyme deactivation condition be：Enzyme deactivation temperature
Spend for 90~125 DEG C, enzymolysis time is 0.5~3h.
9. the preparation method of deer whip active peptide as claimed in claim 1, it is characterised in that in described step F1, special mistake
Filtering auxiliary combination is：Activated carbon, perlite, diatomite, its ratio are 6~7: 1~2: 2~3.
10. a kind of preparation method of deer whip active peptide, it is characterised in that according to any described preparation method of claim 1 to 9
It is made, mass content >=80% of the refined deer whip active peptide, mass content >=30% of the full nutrition deer whip active peptide.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111821401A (en) * | 2020-07-20 | 2020-10-27 | 张培兴 | Enzymolysis deer penis wine for tonifying kidney and strengthening yang and preparation method thereof |
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| CN101748180A (en) * | 2010-01-18 | 2010-06-23 | 山东世纪春食品有限公司 | Functional peanut small molecule mixed polypeptide, preparation method and application thereof |
| CN102174627A (en) * | 2011-01-12 | 2011-09-07 | 武汉百信食品有限公司 | Method for preparing rapeseed bioactive peptide |
| CN103173510A (en) * | 2011-12-21 | 2013-06-26 | 中国科学院大连化学物理研究所 | Preparation method of high-hydroxyproline-content deer collagen peptide |
| CN104789629A (en) * | 2015-04-30 | 2015-07-22 | 中国食品发酵工业研究院 | Chickpea oligopeptide and preparation method thereof |
| CN106962919A (en) * | 2017-02-17 | 2017-07-21 | 舟山赛莱特海洋科技有限公司 | A kind of high-nutrition food for strengthening inoxidizability function and preparation method thereof |
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| CN111821401A (en) * | 2020-07-20 | 2020-10-27 | 张培兴 | Enzymolysis deer penis wine for tonifying kidney and strengthening yang and preparation method thereof |
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