CN107853118A - A kind of cultivation method of flue-cured tobacco small strong seedlings - Google Patents
A kind of cultivation method of flue-cured tobacco small strong seedlings Download PDFInfo
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Abstract
The present invention relates to flue-cured tobacco tobacco seedlings technical field, and in particular to a kind of cultivation method of flue-cured tobacco small strong seedlings, the breeding method comprises the following steps：Tobacco seed is seeded in into seedbed to be cultivated, adjusted the initial Different Nitrogen Concentration of seedbed nutrient solution to 150 200mg/kg when emerging；In foliage spray paclobutrazol in cultivating process.The present invention collaboration other technologies of floating seedling cultivation of fire-cured tobacco, the cured tobacco seedling leaf color for obtaining cultivation is just green, 3 5cm of stalk height, root dry weight >=0.04g/ strains, compared with same time conventional seedbed system mode, root activity improves more than 4.85%, and the visible leaf Net Photosynthetic Rate of top down the 3rd improves more than 17.44%, transplanting survival rate improves more than 3.16%, and seedling time shortening 48h is gone back after transplanting.
Description
Technical field
The present invention relates to flue-cured tobacco tobacco seedlings technical field, in particular to a kind of cultivation method of flue-cured tobacco small strong seedlings.
Background technology
Flue-cured tobacco is a kind of annual herb, belongs to Solanaceae, is a kind of important raw material in tobacco industry.Traditional flue-cured tobacco
The high stem strong sprout cultivated under seedling raising mannerses is advantageous to the raising of tobacco seedlings transplanting quality, but seedling raise period is long, and cigarette district is difficult to adjust
Transplanting time.
The planting system in one region and ecological condition are closely related, if seedling raise period has influence on the flue-cured tobacco transplanting phase, very may be used
It can influence the plantation of other crops.Such as Anhui Province of China south cured tobacco production region, 3 to April, which be mostly that low temperature overcast and rainy day is few, shines
Weather, thereafter into rainy weather, the high temperature dry season after July, in cigarette strain, upper leaf have large effect；And early August
It is the time lower limit for planting late rice, more than this time, the yield and quality of late rice will be by strong influence.Traditional flue-cured tobacco is educated
Seedling mode seedling raise period is longer, influences transplanting time, final to influence planting the time for late rice, destroys the planting system of cigarette-rice crop rotation.
In the prior art, in order to avoid destroying the rotation system of cigarette-rice, gradually the transplanting time of flue-cured tobacco was advanceed in 3 months
In ten days, partly take the area transplanted under seedling film that transplanting time is advanceed into early March.Due to transplanting time in advance, and nursery mould
Formula is substantially unchanged, and tobacco seedlings are smaller, root activity is low, resistance is poor during transplanting, and the tobacco seedlings also seedling time is grown, and survival rate is greatly reduced.
In view of this, it is special to propose the present invention.
The content of the invention
It is an object of the invention to provide a kind of cultivation method of flue-cured tobacco small strong seedlings, described breeding method can promote tobacco seedlings
Growth, the raising of seedling quality, adapt to the nursery pattern that cigarette district is early transplanted.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
A kind of cultivation method of flue-cured tobacco small strong seedlings, comprises the following steps：
Tobacco seed is seeded in seedbed and cultivated, adjusts the initial Different Nitrogen Concentration of seedbed nutrient solution when emerging
To 150-200mg/kg；In foliage spray paclobutrazol in cultivating process.
Preferably, the height of the nutrient solution is 3-5cm；It is furthermore preferred that after tobacco seedlings closing disk, seedbed moistening is kept.Battalion
It is 3-5cm that nutrient solution, which highly controls, can be supplied to tobacco seedlings sufficient moisture and nutrient, and and can controls the humidity in seedbed not well
Cause retarded growth and susceptible disease too high and that cause tobacco seedlings；After tobacco seedlings closing disk, seedbed moistening is kept, can effectively control seedling
The humidity of bed, the reduction seedbed incidence of disease, the growth of promotion flue-cured tobacco tobacco root system, tobacco seedlings are more healthy and strong.
Preferably, each element initial concentration of the nutrient solution before the emergence is 0mg/kg.Before emergence at the beginning of control nutrient solution
Beginning concentration is 0mg/kg, as water, with nutrient solution mineral during reducing whole nursery to the salt damage of tobacco seedlings.
Preferably, after tobacco seedlings closing disk, the Different Nitrogen Concentration of seedbed nutrient solution is adjusted to 100 ± 10mg/kg；More preferably
, after tobacco seedlings closing disk, the Different Nitrogen Concentration of seedbed nutrient solution is adjusted to 100mg/kg.After tobacco seedlings closing disk, tobacco seedlings are carried out in itself
Photosynthesis, so turning down nutrient solution Different Nitrogen Concentration, you can meet the growth of tobacco seedlings, avoid fertilizer damage.
Preferably, in tobacco seedling growth to 31 heart stage of leaf, in foliage spray paclobutrazol.It is furthermore preferred that in foliage spray
100-125mg/kg paclobutrazol.
Paclobutrazol is triazole type plant growth regulator, can weaken plant apical growth advantage, the paclobutrazol of suitable concentration
Root respiration intensity can be improved, reduces aerial part respiratory intensity, promotes root growth and development, promotes the photosynthetic efficiency of blade,
Improve anti-adversity.
Malic acid, also known as 2- hydroxysuccinic acids, the key metabolites of numerous metabolic pathways are participated in plant, such as light and
Intermediate supersession of product and breathing product etc., the nutrition condition of tobacco seedlings can be adjusted, promote growth, improve tobacco seedlings because spraying paclobutrazol
The inhibitory action that Stem high growth slows down.
The cultivation method of flue-cured tobacco small strong seedlings of the present invention, it is 0mg/kg that nutrient solution initial concentration is controlled before emergence, whole to reduce
Nutrient solution mineral is to the salt damagies of tobacco seedlings during individual nursery, 100mg/kg during emergence by the initial concentration of nutrient solution by routine
Bring up to 150-200mg/kg, ensure again to be unlikely to while tobacco seedlings sufficient nutrient to cause fertilizer damage, after tobacco seedlings closing disk, tobacco seedlings sheet
Body carries out photosynthesis, so turning down nutrient solution Different Nitrogen Concentration, the control of nutrient solution elemental height is 3-5cm, can be supplied to cigarette
Seedling sufficient moisture and nutrient, and can control well seedbed humidity will not retarded growth that is too high and causing tobacco seedlings and
Susceptible disease；After tobacco seedlings closing disk, seedbed water evaporation quantity reduces, and keeps seedbed moistening, can effectively control the humidity in seedbed, reduce
The seedbed incidence of disease, the growth of promotion flue-cured tobacco tobacco root system, tobacco seedlings are more healthy and strong；During tobacco seedling growth to 31 heart stage of leaf, sprayed on blade face
Paclobutrazol is spilt, promotes tobacco root system growth, stalk thickening, improves leaf chlorophyll, soluble sugar, soluble protein and free dried meat
Histidine content, strengthen the activity of blade nitrate reductase, it is suppressed that the apical dominance of tobacco seedlings, slow down Stem high growth speed, make
It is just green to cultivate obtained cured tobacco seedling leaf color, functional leaf 3-4 pieces, stalk height 3-5cm, stem girth ＞ 1.5cm, root dry weight >=0.04g/
Strain, compared with same time conventional seedbed system mode, root activity improves more than 4.85%, the 3rd visible leaf Net Photosynthetic Rate of top down
More than 17.44% is improved, transplanting survival rate improves more than 3.16%, and seedling time shortening 48h is gone back after transplanting.
Preferably, the later stage is cultivated in tobacco seedlings, in foliage spray malic acid.It is furthermore preferred that 10-15 days before transplanting, in leaf
Spray malic acid in face.
Preferably, 10-15 days before transplanting, in foliage spray 180-220mg/kg malic acid.It is furthermore preferred that transplanting
First 10-15 days, in foliage spray 200mg/kg malic acid.
The breeding method of the present invention, whether the later stage spray malic acid depending on transplanting demand, and stem high request reaches during for transplanting
More than 6cm tobacco seedlings, before transplanting, the later spray malic acid of tobacco seedling growth, tobacco seedlings stem can be made enough to have up to more than 6cm
Effect improves tobacco seedlings nutrition condition, increases cauline leaf fresh weight, improves seedling quality.
Preferably, when emerging, by nutrient solution must the concentrating of nutrients adjust to Cu 120-150mg/kg, Zn
220-250mg/kg、P 170-200mg/kg、Mu 50-70mg/kg、Mn100-150mg/kg。
Copper is the composition of a variety of oxidizing ferment in plant, relevant with the redox reaction in tobacco seedlings body and respiration,
Promote the metabolism of tobacco seedlings vivo protein and the formation of chlorophyll.
Zinc affects the synthesis of auxin indirectly in crop body, and the growth cellulose content when crop zinc-deficiency in stem and bud subtracts
Few, growth stays cool, and plant is short and small；Zinc is also the activator of many enzymes simultaneously, by being produced to tobacco seedlings carbon and nitrogen metabolism
Wide influence, therefore contribute to photosynthesis；Zinc can also strengthen the resistance of tobacco seedlings simultaneously.
Molybdenum element is the component of azotase and nitrate reductase, and scarce molybdenum can influence the synthesis of protein；Molybdenum can also promote
Enter absorption and conversion from Phos to organophosphor of the crop to phosphorus, promote the conversion of phosphate fertilizer, be easy to absorb, promote phosphorus in tobacco seedlings
Movement.
Manganese element is the catalyst of induction biosynthesis chlorophyll, makes tobacco seedling growth good, increases yield.
The nutrient solution improved according to said ratio, compared with conventional nutraceutical liquid, after cultivating tobacco seedlings, the root system of tobacco seedlings is lived
Power is greatly improved, and tobacco seedlings total quality is improved.Need to illustrate is, proportioning of the invention passes through
The more excellent dosage that substantial amounts of creative experiments optimization is drawn, several necessary nutrient concentration are in this scope only in nutrient solution
It is interior, the tobacco seedlings quality cultivated just is can guarantee that, improves transplanting survival rate, the seedling time is gone back in shortening.
Preferably, N ﹕ P in the nutrient solution2O5﹕ K2O ratio is (18 ± 2) ﹕ (10 ± 2) ﹕ (15 ± 2).According to initial
The concentration adjustment phosphorus of nitrogen, the concentration of potassium, nutrient solution within this range can make tobacco seedling growth good.
Compared with prior art, beneficial effects of the present invention are：
(1) cultivation method of flue-cured tobacco small strong seedlings of the invention, controls the concentration of nutrient solution, ensures that the supply of nutrient solution agrees with seedling
Bed seedling growth and development rule, while ensureing tobacco seedlings adequate nutrients, the generation of the salt-free evil in seedbed and fertilizer damage between nursery stage；
(2) usage amount of nutrient solution is controlled, had both been supplied to tobacco seedlings sufficient moisture and nutrient, and control seedbed well
Humidity will not retarded growth and susceptible disease too high and that cause tobacco seedlings, after tobacco seedlings closing disk, keep seedbed moistening, reduce seedling
The bed incidence of disease, the growth of promotion flue-cured tobacco tobacco root system, tobacco seedlings are more healthy and strong；
(3) paclobutrazol is sprayed, promotes tobacco root system growth, stalk thickening, improves leaf chlorophyll, soluble sugar, solvable
Property albumen and free proline content, strengthen the activity of blade nitrate reductase, it is suppressed that the apical dominance of tobacco seedlings, slow down stem
Seedling height speed, tobacco seedlings total quality get a promotion；
(4) the tobacco seedlings leaf color cultivated of the invention is just green, functional leaf 3-4 pieces, stalk height 3-5cm, stem girth ＞
1.5cm, root dry weight >=0.04g/ strains, compared with same time conventional seedbed system mode, root activity improves more than 4.85%, top down the
3 visible leaf Net Photosynthetic Rates improve more than 17.44%, and transplanting survival rate improves more than 3.16%, and the contracting of seedling time is gone back after transplanting
Short 48h；
(5) malic acid is sprayed in above technical foundation, is effectively improved tobacco seedlings nutrition condition, improved seedling quality, can expire
The transplanting pattern that foot requires to stem height；
(6) breeding method of the invention, the tobacco seedlings cultivated adapt to the nursery pattern that cigarette district is early transplanted；
(7) cultural method of the invention, simple to operate, favorable repeatability.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition suggested according to normal condition or manufacturer are carried out.Agents useful for same or the unreceipted production firm person of instrument, it is
The conventional products that can be obtained by commercially available purchase.
The cultivation method of flue-cured tobacco small strong seedlings of the present invention, by the way of floating seedling cultivation of fire-cured tobacco, specifically presses GB/T 25241-
2010.Equipment requirement in the heating technology referenced patents ZL2015206838718 of seedbed, purpose are to reach GB/T 25241-
Temperature parameter request in 2010.But breeding apparatus structure is not limited to used by cultivation method of flue-cured tobacco small strong seedlings of the present invention
In this, other can reach the purpose of the present invention.
Embodiment 1
Carried out in the following manner at Chizhou City, Anhui Province Dongzhi County Zhang Xi towns floating seedling cultivation of fire-cured tobacco workshop, flue-cured tobacco cultivars are cloud
Cigarette 87：
December 18 tobacco seed is seeded in First Year in seedlings nursing plate, seedlings nursing plate is put and floats on the battalion of nursery pond
Above nutrient solution.Before emergence, tobacco seed is cultivated using water, i.e., nutrient solution initial concentration is 0mg/kg, whole to reduce
For nutrient solution mineral to the salt damage of tobacco seedlings, the height of the nutrient solution is 3-5cm during nursery.Water can meet flue-cured tobacco kind
The demand of the sprouting emergence of son.
When the tobacco seed emergence in seedlings nursing plate, the initial Different Nitrogen Concentration of nutrient solution is adjusted to 150mg/kg.It is described
Several necessary nutritional ingredients include in nutrient solution：N ﹕ P2O5﹕ K2The ﹕ 15, Cu of O=18 ﹕ 102+：150mg/kg、Zn2+：250mg/kg、B3 +:200mg/kg、Mu6+:70mg/kg、Mn2+:130mg/kg、Fe3+：100mg/kg.N ﹕ P2O5﹕ K2The ﹕ 15 of O=18 ﹕ 10 ratio is not
It is limited to this, tobacco seedling growth situation is optimal under this ratio, and the ratio can be adjusted according to the growth demand of flue-cured tobacco tobacco seedlings,
It is other to reach the object of the invention, make the ratio that tobacco seedling growth is good.In the incubation, the temperature of nutrient solution is
20 DEG C or so.
Then in tobacco seedling growth to 31 heart stage of leaf, in tobacco seedlings leaf seedling sprinkling 100mg/kg paclobutrazol, blade surface is made
Cover one layer of atomizing particle.After tobacco seedlings closing disk, the Different Nitrogen Concentration of seedbed nutrient solution is adjusted to 100mg/kg, keeps educating
The seedbed moistening of seed plate.Be sampled analysis the March 20 of Second Year, transplanting on March 20, plant in latter week investigation also seedling stage,
Transplanting survival rate.
Embodiment 2
Carried out in the following manner at Chizhou City, Anhui Province Dongzhi County Zhang Xi towns floating seedling cultivation of fire-cured tobacco workshop, flue-cured tobacco cultivars are cloud
Cigarette 87：
December 18 tobacco seed is seeded in First Year in seedlings nursing plate, seedlings nursing plate is floated on above nutrient solution.Emergence
Before, tobacco seed is cultivated using water, i.e., nutrient solution initial concentration is 0mg/kg, with nutrient solution during reducing whole nursery
For mineral to the salt damage of tobacco seedlings, the height of the nutrient solution is 3-5cm.
When the tobacco seed emergence in seedlings nursing plate, the initial Different Nitrogen Concentration of nutrient solution is adjusted to 200mg/kg.It is described
Several main nutrient compositions include in nutrient solution：N ﹕ P2O5﹕ K2The ﹕ 15, Cu of O=18 ﹕ 102+：150mg/kg、Zn2+：250mg/kg、B3 +:200mg/kg、Mu6+:70mg/kg、Mn2+:130mg/kg、Fe3+：100mg/kg.In the incubation, the temperature of nutrient solution
For 20 DEG C or so.
Then in tobacco seedling growth to 31 heart stage of leaf, in tobacco seedlings leaf seedling sprinkling 100mg/kg paclobutrazol, blade surface is made
Cover one layer of atomizing particle.After tobacco seedlings closing disk, the Different Nitrogen Concentration of seedbed nutrient solution is adjusted to 100mg/kg, keeps educating
The seedbed moistening of seed plate.Be sampled analysis the March 20 of Second Year, transplanting on March 20, plant in latter week investigation also seedling stage,
Transplanting survival rate.
Embodiment 3
Carried out in the following manner at Chizhou City, Anhui Province Dongzhi County Zhang Xi towns floating seedling cultivation of fire-cured tobacco workshop, flue-cured tobacco cultivars are cloud
Cigarette 87：
December 18 tobacco seed is seeded in First Year in seedlings nursing plate, seedlings nursing plate is floated on above nutrient solution.Emergence
Before, tobacco seed is cultivated using water, i.e., nutrient solution initial concentration is 0mg/kg, with nutrient solution during reducing whole nursery
For mineral to the salt damage of tobacco seedlings, the height of the nutrient solution is 3-5cm.
When the tobacco seed emergence in seedlings nursing plate, the initial Different Nitrogen Concentration of nutrient solution is adjusted to 150mg/kg.It is described
Several main nutrient compositions include in nutrient solution：N ﹕ P2O5﹕ K2The ﹕ 15, Cu of O=18 ﹕ 102+：150mg/kg、Zn2+：250mg/kg、B3 +:200mg/kg、Mu6+:70mg/kg、Mn2+:130mg/kg、Fe3+：100mg/kg.In the incubation, the temperature of nutrient solution
For 20 DEG C or so.
Then in tobacco seedling growth to 31 heart stage of leaf, in tobacco seedlings leaf seedling sprinkling 125mg/kg paclobutrazol, blade surface is made
Cover one layer of atomizing particle.After tobacco seedlings closing disk, the Different Nitrogen Concentration of seedbed nutrient solution is adjusted to 100mg/kg, keeps educating
The seedbed moistening of seed plate.10-15 days before transplanting, in foliage spray 200mg/kg malic acid, blade surface is set to cover one layer
Atomizing particle.It is sampled analysis, transplanting on March 20, investigation also seedling stage, shifting in cultivation latter week the March 20 of Second Year
Plant survival rate.
Embodiment 4
Carried out in the following manner at Chizhou City, Anhui Province Dongzhi County Zhang Xi towns floating seedling cultivation of fire-cured tobacco workshop, flue-cured tobacco cultivars are cloud
Cigarette 87：
December 18 tobacco seed is seeded in First Year in seedlings nursing plate, seedlings nursing plate is floated on above nutrient solution.Emergence
Before, tobacco seed is cultivated using water, i.e., nutrient solution initial concentration is 0mg/kg, with nutrient solution during reducing whole nursery
For mineral to the salt damage of tobacco seedlings, the height of the nutrient solution is 3-5cm.
When the tobacco seed emergence in seedlings nursing plate, the initial Different Nitrogen Concentration of nutrient solution is adjusted to 200mg/kg.It is described
Several main nutrient compositions include in nutrient solution：N ﹕ P2O5﹕ K2The ﹕ 15, Cu of O=18 ﹕ 102+：150mg/kg、Zn2+：250mg/kg、B3 +:200mg/kg、Mu6+:70mg/kg、Mn2+:130mg/kg、Fe3+：100mg/kg.In the incubation, the temperature of nutrient solution
For 20 DEG C or so.
Then in tobacco seedling growth to 31 heart stage of leaf, in tobacco seedlings leaf seedling sprinkling 125mg/kg paclobutrazol, blade surface is made
Cover one layer of atomizing particle.After tobacco seedlings closing disk, the Different Nitrogen Concentration of seedbed nutrient solution is adjusted to 100mg/kg, keeps educating
The seedbed moistening of seed plate.10-15 days before transplanting, in foliage spray 200mg/kg malic acid, blade surface is set to cover one layer
Atomizing particle.It is sampled analysis, transplanting on March 20, investigation also seedling stage, shifting in cultivation latter week the March 20 of Second Year
Plant survival rate.
Comparative example 1
With Chizhou City, Anhui Province cigarette district routine floating seedling cultivation of fire-cured tobacco technology, flue-cured tobacco tobacco seedlings are cultivated, flue-cured tobacco cultivars are cloud and mist 87,
In First Year sowing on December 18, in Second Year sampling analysis on March 20, transplanting on March 20, plant investigation in latter week and go back seedling
Phase, transplanting survival rate.
Experimental example 1
Compare the breeding method of embodiments of the invention 1-4 and comparative example 1, analyze and early transplant local dwelling in Wannan is preferably
Tobacco seedlings cultivate pattern.
Compare the breeding method of embodiments of the invention 1-4 and comparative example 1, to flue-cured tobacco tobacco seedlings also seedling and transplanting survival rate
Influence, concrete outcome is referring to table 1.
Influence of the 1 different breeding methods of table to tobacco seedlings also seedling stage, transplanting survival rate
| Processing | Sowing time | Also seedling stage (h) | Transplanting survival rate (%) |
| Embodiment 1 | December 18 | 96 | 98.21a |
| Embodiment 2 | December 18 | 96 | 97.53a |
| Embodiment 3 | December 18 | 96 | 97.48a |
| Embodiment 4 | December 18 | 96 | 97.85a |
| Comparative example 1 | December 18 | 144 | 94.37b |
As can be seen from Table 1, breeding method of the invention goes back the seedling time after can effectively shortening transplanting, compared to tradition
Breeding method, 48h can be shortened, transplanting survival rate is significantly higher than comparative example 1, increase rate 3.30%-4.01%.
The conventional seedbed system of cultivation method of flue-cured tobacco small strong seedlings and comparative example 1 described in comparing embodiment 1-4 terminates to flue-cured tobacco
The influence of the function number of sheets and Net Photosynthetic Rate, the Net Photosynthetic Rate of the 3rd visible leaf of top down is determined, concrete outcome is referring to table
2。
Influence of the 2 different breeding methods of table to the tobacco seedlings function number of sheets and Net Photosynthetic Rate
| Processing | The function number of sheets (piece) | Net Photosynthetic Rate |
| Embodiment 1 | 3.4a | 14.268a |
| Embodiment 2 | 3.7a | 14.375a |
| Embodiment 3 | 3.6a | 14.368a |
| Embodiment 4 | 3.5a | 14.581a |
| Comparative example 1 | 4.3a | 12.149b |
It was found from from upper table 2, compared with traditional breeding method in comparative example 1, through the present invention's
The tobacco seedlings functional leaf 3-4 pieces of embodiment 1-4 breeding method processing, with comparative example 1 without significant difference；Top down
3rd visible leaf Net Photosynthetic Rate is significantly higher than comparative example 1, is improved compared with comparative example 1 up to more than 17.44%, can effectively improve shifting
Plant the growth rate of survival rate and tobacco seedlings.
The conventional seedbed system of cultivation method of flue-cured tobacco small strong seedlings and comparative example 1 described in comparing embodiment 1-4 terminates to flue-cured tobacco
The influence of tobacco seedlings economical character, and the influence of the tobacco root system vigor to flue-cured tobacco, nitrate reductase activity and MDA contents, tool
Body result is referring to table 3-4.
Influence of the 3 different breeding methods of table to tobacco seedlings economical character
Influence of the 4 different breeding methods of table to tobacco root system vigor, nitrate reductase activity and MDA contents
Note：In table 1-4 with after column data carry different lowercases mark difference reach 0.05 the level of signifiance.
It was found from from upper table 3 and table 4, compared with traditional breeding method in comparative example 1, through embodiments of the invention 1-
The tobacco seedlings of 4 breeding method processing stem is wide, root fresh weight and root dry weight dramatically increase, stem is wide to be added relative to comparative example
5.33%-13.89%, root fresh weight add 14.93%-22.54% without significant difference, root dry weight.Stem height, cauline leaf fresh weight and stem
Leaf dry weight decreases compared with comparative example 1.And embodiment 3 and embodiment 4, carry out spraying 200mg/kg malic acid respectively in the later stage
Processing, stem height is significantly increased, more than 6cm.
Compared with traditional breeding method in comparative example 1, the breeding method through embodiments of the invention 1-4 handles to obtain
Tobacco root system vigor and nitrate reductase significantly improved compared with comparative example, tobacco root system vigor increase rate reaches 9.90%-
14.74%, tobacco seedlings nitrate reductase activity increase rate reaches 12.01%-29.64%, embodiment 1-4 tobacco seedlings blade malonic acid
(MDA) content is substantially less than the malonic acid of blade in comparative example 1, and the amplitude of reduction reaches 12.85%-19.12%.
Although illustrate and describing the present invention with specific embodiment, but will be appreciated that without departing substantially from the present invention's
Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of cultivation method of flue-cured tobacco small strong seedlings, it is characterised in that comprise the following steps：
Tobacco seed is seeded in into seedbed to be cultivated, adjusted the initial Different Nitrogen Concentration of seedbed nutrient solution to 150- when emerging
200mg/kg；In foliage spray paclobutrazol in cultivating process.
2. cultivation method of flue-cured tobacco small strong seedlings according to claim 1, it is characterised in that the height of the nutrient solution is 3-
5cm；It is furthermore preferred that after tobacco seedlings closing disk, seedbed moistening is kept.
3. cultivation method of flue-cured tobacco small strong seedlings according to claim 1, it is characterised in that nutrient solution before the emergence it is each
Element initial concentration is 0mg/kg.
4. cultivation method of flue-cured tobacco small strong seedlings according to claim 1, it is characterised in that after tobacco seedlings closing disk, seedbed is sought
The Different Nitrogen Concentration of nutrient solution is adjusted to 100 ± 10mg/kg；
Preferably, after tobacco seedlings closing disk, the Different Nitrogen Concentration of seedbed nutrient solution is adjusted to 100mg/kg.
5. cultivation method of flue-cured tobacco small strong seedlings according to claim 1, it is characterised in that in tobacco seedling growth to the heart stage of 3 leaf 1
When, in foliage spray paclobutrazol；
Preferably, in foliage spray 100-125mg/kg paclobutrazol.
6. according to the cultivation method of flue-cured tobacco small strong seedlings described in claim any one of 1-5, it is characterised in that after tobacco seedlings cultivation
Phase, in foliage spray malic acid.
7. cultivation method of flue-cured tobacco small strong seedlings according to claim 6, it is characterised in that 10-15 days before transplanting, on blade face
Spray malic acid.
8. cultivation method of flue-cured tobacco small strong seedlings according to claim 7, it is characterised in that 10-15 days before transplanting, on blade face
Spray 180-220mg/kg malic acid；
Preferably, 10-15 days before transplanting, in foliage spray 200mg/kg malic acid.
9. cultivation method of flue-cured tobacco small strong seedlings according to claim 1, it is characterised in that when emerging, nutrient solution is necessary
The concentrating of nutrients is adjusted to Cu 120-150mg/kg, Zn 220-250mg/kg, P 170-200mg/kg, Mu 50-
70mg/kg、Mn100-150mg/kg。
10. cultivation method of flue-cured tobacco small strong seedlings according to claim 1, it is characterised in that N ﹕ P in the nutrient solution2O5﹕ K2O
Ratio be (18 ± 2) ﹕ (10 ± 2) ﹕ (15 ± 2).
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