CN107827615A - A kind of microbial-bacterial fertilizer for promoting arboreal growth and preparation method thereof - Google Patents
A kind of microbial-bacterial fertilizer for promoting arboreal growth and preparation method thereof Download PDFInfo
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- CN107827615A CN107827615A CN201711341483.1A CN201711341483A CN107827615A CN 107827615 A CN107827615 A CN 107827615A CN 201711341483 A CN201711341483 A CN 201711341483A CN 107827615 A CN107827615 A CN 107827615A
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- microbial
- bacterial fertilizer
- agrobacterium rhizogenes
- biomass carbon
- growth
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 43
- 230000012010 growth Effects 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 230000001737 promoting effect Effects 0.000 title claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 31
- 241000589156 Agrobacterium rhizogenes Species 0.000 claims abstract description 27
- 239000002028 Biomass Substances 0.000 claims abstract description 23
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 23
- 239000005416 organic matter Substances 0.000 claims abstract description 20
- 241000589158 Agrobacterium Species 0.000 claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 235000009973 maize Nutrition 0.000 claims description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims description 3
- 241000219793 Trifolium Species 0.000 claims description 3
- 239000002154 agricultural waste Substances 0.000 claims description 3
- 239000010828 animal waste Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000010902 straw Substances 0.000 claims description 3
- 241001037822 Bacillus bacterium Species 0.000 claims 1
- 238000003763 carbonization Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 19
- 210000003608 fece Anatomy 0.000 abstract description 12
- 239000010871 livestock manure Substances 0.000 abstract description 12
- 230000001580 bacterial effect Effects 0.000 abstract description 11
- 241000124033 Salix Species 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 235000008331 Pinus X rigitaeda Nutrition 0.000 abstract description 2
- 241000018646 Pinus brutia Species 0.000 abstract description 2
- 235000011613 Pinus brutia Nutrition 0.000 abstract description 2
- 244000046101 Sophora japonica Species 0.000 abstract description 2
- 235000010586 Sophora japonica Nutrition 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 230000002786 root growth Effects 0.000 abstract description 2
- 241000218691 Cupressaceae Species 0.000 abstract 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 abstract 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 15
- 229910052709 silver Inorganic materials 0.000 description 15
- 239000004332 silver Substances 0.000 description 15
- 241000219000 Populus Species 0.000 description 13
- 238000000034 method Methods 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 3
- 230000008092 positive effect Effects 0.000 description 3
- 230000004913 activation Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000015816 nutrient absorption Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 241001503987 Clematis vitalba Species 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241001633102 Rhizobiaceae Species 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to a kind of microbial-bacterial fertilizer for promoting arboreal growth and preparation method thereof, wherein trees mainly include willow, pine tree, cypress, willow and Chinese scholartree etc., and it with Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 is made of primary raw material that the bacterial manure, which is,.Agrobacterium rhizogenes (Agrobacterium rhizogenes) has the function that to promote plant establishment growth, there is important influence to growth and development of plants, therefore, use OD values to be mixed for 0.4 0.6 agrobacterium rhizogenes bacterium solution with organic matter composition and biomass carbon and microbial-bacterial fertilizer is made, topdress 15cm trees seedling, because biological bacteria and plant form a kind of symbiosis, the root growth of trees can be stimulated and increase root absorption area, enhance the rootability of trees, improve the nutrient efficiency of trees, promote arboreal growth, overcome unsound problem of growing during arboreal growth, played a great role in afforestation.
Description
Technical field
The present invention relates to a kind of microbial-bacterial fertilizer for promoting arboreal growth and preparation method thereof, belong to technical field of forestry.
Background technology
Trees are a kind of tall and big woody tissue plants, also have " leaf " presentation by " branch " and " bar ", can survive decades.One
As arbor is referred to as setting, have obvious upright trunk, plant is typically tall and big, and branch is higher apart from ground, can form tree crown.Tree
Wood is the general name of xylophyta, has point of arbor, shrub and woody climber, trees are mainly seed plant, and China there are about 8000
Kind trees, mainly including willow, pine tree, cypress, willow and Chinese scholartree etc..
Microbial-bacterial fertilizer is according to soil micro-ecosystem principle, Plant Nutrition principle and modern times " organic agriculture "
Basic conception and develop.Microbial manure is to cause crop to obtain required nutrient with the vital movement of active microorganism
A kind of new-type fertilizer biological products.Microbial-bacterial fertilizer, which contains up to more than ten, plants high-efficiency activated beneficial microbe bacterium, suitable for various
Crop uses, and can activate nutrient, improves nutrient utilization rate, has universal, has broken " selectivity " " limitation of common bio-fertilizer
The intrinsic weakness of property " " special fertilizer ", this is that other bio-feritlizers are incomparable.
Agrobacterium rhizogenes belongs to the Gram-negative bacteria of Agrobacterium in Rhizobiaceae, after plant is infected, can induce
Plant produces a large amount of hyperbranched adventitious roots, commonly referred to as root of hair.Root of hair has that the speed of growth is fast, differentiation degree is high, physiology
Biochemical and genetic stability, it is easy to the features such as progress operational control.After agrobacterium rhizogenes processing, improve plant establishment rate, root
Increment increase, the enhancing of root system can significantly improve the survival rate of plant, and well developed root system can improve the foster of plant
Part absorption efficiency, promotes growing for plant.
The content of the invention
For above-mentioned phenomenon, a kind of microbial-bacterial fertilizer for promoting arboreal growth is there is provided, the microbial-bacterial fertilizer utilizes hair
Root Agrobacterium it is distinctive promote plant root growth and strengthen rootability effect, solve trees in growth course due to
Survival rate is low caused by root system is undeveloped, unsound problem of growing, and the invention provides one kind to promote arboreal growth
Microbial-bacterial fertilizer and preparation method thereof.
The present invention is realized by following technical scheme：
A kind of microbial-bacterial fertilizer for promoting arboreal growth, it is characterised in that：Described microbial-bacterial fertilizer is matched somebody with somebody by following component
The raw material of ratio is made：
Agrobacterium rhizogenes bacterium solution 30ml
Organic matter composition 800g
Biomass carbon 200g
Strain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more in 15834, strain derive from Chinese microorganism strain collection；
Described agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind in animal wastes
It is or several；
The biomass carbon refers to that timber, grass, maize straw or other agricultural waste materials pass through height under low-oxygen environment
Anneal crack solution is carbonized the material to be formed.
A kind of preparation method of the microbial-bacterial fertilizer of promotion arboreal growth, it is characterised in that：This method comprises the following steps：
1) preparation of agrobacterium rhizogenes bacterium solution, by Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
One or more of activation to OD values be 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.
2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon
4:1 is well mixed, obtains the mixture of organic matter composition and biomass carbon.
3) hair that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2)
Root Agrobacterium bacterium solution uniform stirring, the mixture of every 1kg organic matters composition and biomass carbon add bacterium solution 30ml, obtain microorganism
Bacterial manure.
The present invention has following positive effect：
1st, the microorganism fungus kind that microbial-bacterial fertilizer of the present invention uses for Agrobacterium rhizogenesA4, R1205, R1000, R1601,
One or more in R1022,15834, agrobacterium rhizogenes can induce trees and produce hairy root, promote the growth of tree root,
Play the role of to the nutrient absorption efficiency and survival rate for improving trees important.
2nd, microbial-bacterial fertilizer of the present invention uses agrobacterium rhizogenes bacterium solution, and organic matter composition and biomass carbon are former material
Material, there is the advantages of cost is relatively low, easy to obtain, preparation method is simple, be readily put into production.
3rd, arboreal growth is applied to using microbial-bacterial fertilizer of a kind of promotion arboreal growth of the present invention and preparation method thereof,
Solve the problems, such as that plant survival rate caused by root system is undeveloped in growth course is low, there is huge economic benefit.
Embodiment
With reference to case study on implementation, the invention will be further described, mainly by taking poplar plant in silver as an example, does not limit this hair
The bright scope of application：
A kind of microbial-bacterial fertilizer for promoting Yang Shengchang in silver, the raw material system that described microbial-bacterial fertilizer is matched by following component
Into：
Agrobacterium rhizogenes bacterium solution 30ml
Organic matter composition 800g
Biomass carbon 200g
Strain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more in 15834, strain derive from Chinese microorganism strain collection；
Described agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind in animal wastes
It is or several；
The biomass carbon refers to that timber, grass, maize straw or other agricultural waste materials pass through height under low-oxygen environment
Anneal crack solution is carbonized the material to be formed.
A kind of preparation method for the microbial-bacterial fertilizer for promoting capsicum to take root, this method comprise the following steps：
1) preparation of agrobacterium rhizogenes bacterium solution, by Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
One or more of activation to OD values be 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.
2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon
4:1 is well mixed, obtains the mixture of organic matter composition and biomass carbon.
3) hair that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2)
Root Agrobacterium bacterium solution uniform stirring, the mixture of every 1kg organic matters composition and biomass carbon add bacterium solution 30ml, obtain microorganism
Bacterial manure.
Effective viable bacteria >=3 × 10 of described microbial-bacterial fertilizer8cuf/g。
Promote the application process of arboreal growth
The seedling of trees plant is planted, fertilizer is uniformly distributed in by soil plough horizon, 1kg/ using whole layer placement of fertilizer method
m2, apply fertilizer once within every 30 days, there is obvious well developed root system and eugonic feature in trees developmental process, substantially increase
The quality and ornamental value of trees.
Microbial-bacterial fertilizer based on agrobacterium rhizogenes is applied in silver in poplar arboreal growth
60 plants of the Yang Youmiao in the silver for buying 15cm on the market, every 30 plants are one group of plantation, are divided into control group and experimental group,
Control group does not apply fertilizer, and experimental group fertilising, is about applied fertilizer 1kg, about 1kg/m using every ten plants of seedling of whole layer placement of fertilizer method2, apply fertilizer within every 30 days
Once, apply fertilizer 3 times, observe and record the growth conditions of poplar plant in silver.It is as shown in the table to record result：
Poplar plant strain growth situation in the silver of different number of days
As a result show, poplar plant is compared in the silver less to apply fertilizer, applies microbial-bacterial fertilizer based on agrobacterium rhizogenes
Poplar has obvious survival rate height, well developed root system, eugonic feature in developmental process in silver.Result explanation, this hair
A kind of preparation method of the microbial-bacterial fertilizer of bright promotion arboreal growth improves the growth quality of trees, has emphatically to environmental greening
The positive effect wanted.
Table 1 is growth phenotype ginseng of the poplar under different number of days in application bacterial manure of the present invention and the silver for not applying bacterial manure of the present invention
Several changes.
Table 1 applies influence of the bacterial manure to poplar growth phenotype in silver
Note：At different number of days (10d, 20d, 30d), do not apply poplar (control group) in bacterial manure silver and apply poplar reality in bacterial manure silver
Test group) plant height of seedling, single-strain blade quantity and SL A change.
The change of the growth phenotype of poplar plant in the silver of bacterial manure is less applied by poplar plant in the silver of measure application bacterial manure, is obtained
The data arrived further illustrate that be applied with poplar in the silver with bacterial manure has obvious eugonic feature in developmental process.
A kind of preparation method of the microbial-bacterial fertilizer of promotion arboreal growth of the present invention makes the well developed root system of trees, and then improves trees
Nutrient absorption efficiency, growing for trees is promoted, there is important positive effect to environmental greening.
Claims (4)
- A kind of 1. microbial-bacterial fertilizer for promoting arboreal growth, it is characterised in that：Described microbial-bacterial fertilizer is matched by following component Raw material be made：Agrobacterium rhizogenes bacterium solution 30mlOrganic matter composition 800gBiomass carbon 200gStrain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022, One or more in 15834, strain derive from Chinese microorganism strain collection；The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind or several in animal wastes Kind；The biomass carbon refers to timber, grass, maize straw or other agricultural waste materials under low-oxygen environment by high anneal crack The material that solution carbonization is formed.
- A kind of 2. microbial-bacterial fertilizer for promoting arboreal growth according to claim 1, it is characterised in that：Described Agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes that OD values are 0.4-0.6.
- A kind of 3. preparation method of the microbial-bacterial fertilizer of promotion arboreal growth according to claim 1, it is characterised in that：Its Preparation method comprises the following steps：1) preparation of agrobacterium rhizogenes bacterium solution, by one in Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 Kind or several activate to OD values are 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon 4:1 is mixed Close the mixture for uniformly, obtaining organic matter composition and biomass carbon.3) the root of hair agriculture that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2) Bacillus bacterium solution uniform stirring, the mixture of every 1kg organic matters and biomass carbon add bacterium solution 30ml, obtain microbial-bacterial fertilizer.
- A kind of 4. preparation method of the microbial-bacterial fertilizer of promotion arboreal growth according to claim 3, it is characterised in that institute Effective viable bacteria >=2 × 10 of microbial-bacterial fertilizer stated8cuf/g。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711341483.1A CN107827615A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer for promoting arboreal growth and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711341483.1A CN107827615A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer for promoting arboreal growth and preparation method thereof |
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| CN107827615A true CN107827615A (en) | 2018-03-23 |
Family
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|---|---|---|---|
| CN201711341483.1A Pending CN107827615A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer for promoting arboreal growth and preparation method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100115006A (en) * | 2009-04-17 | 2010-10-27 | 충북대학교 산학협력단 | Bacterial strain cbcn5 promoting plant growth isolated from rhizosphere soils of chinese cabbage and uses thereof |
| CN107011050A (en) * | 2017-04-20 | 2017-08-04 | 内蒙古民族大学 | A kind of charcoal base dissolving phosphor and dissolving potassium bacterial manure and preparation method thereof |
| CN107278590A (en) * | 2017-07-28 | 2017-10-24 | 周运德 | Improve ginsenoside Rb in cultivation field seven1The method of content |
-
2017
- 2017-12-14 CN CN201711341483.1A patent/CN107827615A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100115006A (en) * | 2009-04-17 | 2010-10-27 | 충북대학교 산학협력단 | Bacterial strain cbcn5 promoting plant growth isolated from rhizosphere soils of chinese cabbage and uses thereof |
| CN107011050A (en) * | 2017-04-20 | 2017-08-04 | 内蒙古民族大学 | A kind of charcoal base dissolving phosphor and dissolving potassium bacterial manure and preparation method thereof |
| CN107278590A (en) * | 2017-07-28 | 2017-10-24 | 周运德 | Improve ginsenoside Rb in cultivation field seven1The method of content |
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Application publication date: 20180323 |
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