CN107827614A - A kind of microbial-bacterial fertilizer for promoting capsicum to take root and preparation method thereof - Google Patents
A kind of microbial-bacterial fertilizer for promoting capsicum to take root and preparation method thereof Download PDFInfo
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- CN107827614A CN107827614A CN201711341482.7A CN201711341482A CN107827614A CN 107827614 A CN107827614 A CN 107827614A CN 201711341482 A CN201711341482 A CN 201711341482A CN 107827614 A CN107827614 A CN 107827614A
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- capsicum
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- bacterial fertilizer
- bacterium solution
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
Promote microbial-bacterial fertilizer for taking root of capsicum and preparation method thereof the present invention relates to a kind of, it with Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 is made of primary raw material that the bacterial manure, which is,.Agrobacterium rhizogenes (Agrobacterium rhizogenes) has the function that to promote plant establishment growth, there is important influence to growth and development of plants, therefore, use OD values to be mixed for 0.4 0.6 agrobacterium rhizogenes bacterium solution with organic matter composition and biomass carbon and microbial-bacterial fertilizer is made, two are topdressed to tri-leaf period pepper seedling, because biological bacteria and capsicum form a kind of symbiosis, stimulate the root growth of capsicum and increase root absorption area, improve the nutrient efficiency of capsicum, resist capsicum root system it is unsound phenomena such as, promote chili growth development, it is low to overcome fruit quality in capsicum production, the problem of yielding poorly, it agriculturally can be used for improving the quality of capsicum and increasing its yield, there is huge economic benefit.
Description
Technical field
The present invention relates to a kind of microbial-bacterial fertilizer for promoting capsicum to take root and preparation method thereof, belong to agricultural technology field.
Background technology
Capsicum (Capsicum annuum L.) is Solanaceae, 1 year of Capsicum or limited herbaceos perennial, capsicum
Generally cultivated all over China, can be planted in neutral and subacidity soil, turn into a kind of popular vegetables.Capsicum is growing
During capsicum can usually occur in growing blade turn yellow, leaf roll, or even the physiological phenomenon such as withered；Capsicum root system is not sent out
Reach, be mainly shown as that main root is thick, root amount is few, and root growth is slow；And the disease getting worse such as pepper ralstonia solanacearum, cause root system
Phenomena such as unsound.These problems often result in the problem of crops final growth quality and yield reduction, in order to improve
The quality of crops, increase crop yield, in 20 end of the centurys, the development of microbial-bacterial fertilizer is arisen at the historic moment.
Microbial-bacterial fertilizer is according to soil micro-ecosystem principle, Plant Nutrition principle and modern times " organic agriculture "
Basic conception and develop.Microbial manure is to cause crop to obtain required nutrient with the vital movement of active microorganism
A kind of new-type fertilizer biological products.Microbial-bacterial fertilizer, which contains up to more than ten, plants high-efficiency activated beneficial microbe bacterium, suitable for various
Crop uses, and can activate nutrient, improves nutrient utilization rate, has universal, has broken " selectivity " " limitation of common bio-fertilizer
The intrinsic weakness of property " " special fertilizer ", this is that other bio-feritlizers are incomparable.Microbial-bacterial fertilizer is the new of 21st century
Fertilizer, it is a kind of low charcoal, pure natural, nontoxic, harmless, free of contamination biological organic fertilizer, can increase soil fertility, improve soil
Character is activated, is kept soil from packing together；Soil fertilizer, water conservation are improved, cold tolerance, strengthens crop disease-resistant ability, is increased in soil
Organic matter, prevent pathogen invade, reduce plant pest and disease damage growth.
Agrobacterium rhizogenes, which infects rear plant, can produce many hairy roots, consolidate root system of plant, promote growth and development of plants, because
This prepares microbial-bacterial fertilizer using agrobacterium rhizogenes bacterium solution, and growing for plant is had great significance, raw in agricultural
In production, there is potential economic benefit.
The content of the invention
For above-mentioned phenomenon, it is an object of the invention to provide a kind of microbial-bacterial fertilizer for promoting capsicum to take root, micro- life
Thing bacterial manure solves capsicum in life using the distinctive effect for promoting plant root growth and strengthening rootability of agrobacterium rhizogenes
In growth process due to survival rate is low, fruit quality is low caused by root system is undeveloped, yield poorly the problem of, the invention provides one
The preparation for the microbial-bacterial fertilizer that kind promotion capsicum takes root.
The present invention is realized by following technical scheme：
A kind of microbial-bacterial fertilizer for promoting capsicum to take root, the raw material system that described microbial-bacterial fertilizer is matched by following component
Into：
Agrobacterium rhizogenes bacterium solution 15ml
Organic matter composition 400g
Biomass carbon 100g
Strain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more in 15834, strain derive from Chinese microorganism strain collection；
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind in animal wastes
It is or several；
The biomass carbon refers to that timber, grass, maize straw or other agricultural waste materials pass through height under low-oxygen environment
Anneal crack solution is carbonized the material to be formed.
Described agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
A kind of preparation method for the microbial-bacterial fertilizer for promoting capsicum to take root, it is characterised in that：This method comprises the following steps：
1) preparation of agrobacterium rhizogenes bacterium solution, by Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
One or more of activation to OD values be 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.
2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon
4:1 is well mixed, obtains the mixture of organic matter composition and biomass carbon.
3) hair that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2)
Root Agrobacterium bacterium solution uniform stirring, the mixture of every 500g organic matters composition and biomass carbon add bacterium solution 15ml, obtain micro- life
Thing bacterial manure.
The present invention has following positive effect：
1st, the microorganism fungus kind that microbial-bacterial fertilizer of the present invention uses for agrobacterium rhizogenes strain A4, R1205, R1000,
One or more in R1601, R1022,15834, agrobacterium rhizogenes can induce capsicum and produce hairy root, promote capsicum root system
Growth, play the role of to the nutrient absorption efficiency for improving capsicum important.
2nd, microbial-bacterial fertilizer of the present invention uses agrobacterium rhizogenes bacterium solution, and organic matter composition and biomass carbon are former material
Material, there is the advantages of cost is relatively low, easy to obtain, preparation method is simple, be readily put into production.
3rd, the preparation method for the microbial-bacterial fertilizer taken root using a kind of promotion capsicum of the present invention is applied to the production of capsicum,
Solve the problems, such as capsicum in growth course due to root system it is undeveloped caused by survival rate is low, fruit quality is low, yields poorly, have
Huge economic benefit.
Brief description of the drawings
Fig. 1 is to apply bacterial manure of the present invention and do not apply Root Parameters cylindricality of the capsicum of bacterial manure of the present invention under different number of days
Figure.
Embodiment
With reference to case study on implementation, the invention will be further described：
A kind of microbial-bacterial fertilizer for promoting capsicum to take root, the raw material system that described microbial-bacterial fertilizer is matched by following component
Into：
Agrobacterium rhizogenes bacterium solution 15ml
Organic matter composition 400g
Biomass carbon 100g
Strain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more in 15834, strain derive from Chinese microorganism strain collection；
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind in animal wastes
It is or several；
The biomass carbon refers to that timber, grass, maize straw or other agricultural waste materials pass through height under low-oxygen environment
Anneal crack solution is carbonized the material to be formed.
Described agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
A kind of preparation method for the microbial-bacterial fertilizer for promoting capsicum to take root, this method comprise the following steps：
1) preparation of agrobacterium rhizogenes bacterium solution, by Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
One or more of activation to OD values be 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.
2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon
4:1 is well mixed, obtains the mixture of organic matter composition and biomass carbon.
3) hair that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2)
Root Agrobacterium bacterium solution uniform stirring, the mixture of every 500g organic matters composition and biomass carbon add bacterium solution 15ml, obtain micro- life
Thing bacterial manure.
Effective viable bacteria >=3 × 10 of described microbial-bacterial fertilizer8cuf/g。
The application process for promoting capsicum to take root
Two to tri-leaf period pepper seedling is planted, fertilizer is uniformly distributed in by soil plough horizon using whole layer placement of fertilizer method,
About 500g/m2, apply fertilizer within every 10 days once up to having obvious well developed root system in result, pepper plant developmental process, grow prosperous
Contain, the characteristics of setting percentage is high, substantially increase the quality and yield of capsicum.
Microbial-bacterial fertilizer based on agrobacterium rhizogenes is in capsicum production application
In 60 plants of pepper seedling for buying tri-leaf period on the market, every 30 plants are one group of plantation, are divided into control group and experimental group,
Control group is not applied fertilizer, and experimental group fertilising, is about applied fertilizer 500g using every ten plants of seedling of whole layer placement of fertilizer method, and fertilising in every 10 days once, is applied
Fertilizer 3 times, observes and records Reducing sugar.Record result such as table 1：
The pepper plant growing state of the different number of days of table 1
As a result show, the capsicum for the microbial-bacterial fertilizer being applied with based on agrobacterium rhizogenes has substantially in developmental process
Survival rate it is high, well developed root system, the characteristics of growth is vigorous, and setting percentage is high, the plant less applied fertilizer is compared, Quality of Capsicum and yield
Increase, result explanation, a kind of microbial-bacterial fertilizer based on agrobacterium rhizogenes for promoting capsicum to take root of the present invention and
Its preparation method, the growth quality of crops is improved, there is important positive effect to agricultural production.
Shown in accompanying drawing 1：In different number of days (I:10d、II:20d、III:30d), do not apply bacterial manure capsicum (control group) and apply
Add the total root long of plant (cm/ strains), the root surface area (cm of bacterial manure capsicum (experimental group)2), root volume (cm3), average root diameter
(mm), tip of a root number (bar/strain) and the change of radical (bar/strain) is divided.
The root growth of the soybean plant strain of bacterial manure is less applied by the soybean plant strain of root scanner measure application bacterial manure
Situation of change, further illustrating, the capsicum for applying bacterial manure has obvious well developed root system in developmental process, and rootability is strong,
Eugonic feature, the plant less applied fertilizer are compared, and the nutrient absorption efficiency of capsicum increases.One kind of the invention is with root of hair
Microbial-bacterial fertilizer based on Agrobacterium improves the growth quality of capsicum, and the production to capsicum has important positive effect.
Claims (4)
- A kind of 1. microbial-bacterial fertilizer for promoting capsicum to take root, it is characterised in that：Described microbial-bacterial fertilizer is matched by following component Raw material be made：Agrobacterium rhizogenes bacterium solution 15mlOrganic matter composition 400gBiomass carbon 100gStrain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022, One or more in 15834, strain derive from Chinese microorganism strain collection；The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind or several in animal wastes Kind；The biomass carbon refers to timber, grass, maize straw or other agricultural waste materials under low-oxygen environment by high anneal crack The material that solution carbonization is formed.
- A kind of 2. microbial-bacterial fertilizer for promoting capsicum to take root according to claim 1, it is characterised in that：Described root of hair agriculture Bacillus bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
- A kind of 3. preparation method of microbial-bacterial fertilizer for promoting capsicum to take root according to claim 1, it is characterised in that：Should Method comprises the following steps：1) preparation of agrobacterium rhizogenes bacterium solution, by one in Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 Kind or several activate to OD values are 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon 4:1 is mixed Close the mixture for uniformly, obtaining organic matter composition and biomass carbon.3) the root of hair agriculture that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2) Bacillus bacterium solution uniform stirring, the mixture of every 500g organic matters composition and biomass carbon add bacterium solution 15ml, obtain microbial bacteria Fertilizer.
- A kind of 4. preparation method of microbial-bacterial fertilizer for promoting capsicum to take root according to claim 3, it is characterised in that：Institute Effective viable bacteria >=3 × 10 of microbial-bacterial fertilizer stated8cuf/g。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711341482.7A CN107827614A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer for promoting capsicum to take root and preparation method thereof |
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| CN201711341482.7A CN107827614A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer for promoting capsicum to take root and preparation method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107011050A (en) * | 2017-04-20 | 2017-08-04 | 内蒙古民族大学 | A kind of charcoal base dissolving phosphor and dissolving potassium bacterial manure and preparation method thereof |
| CN107278590A (en) * | 2017-07-28 | 2017-10-24 | 周运德 | Improve ginsenoside Rb in cultivation field seven1The method of content |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107011050A (en) * | 2017-04-20 | 2017-08-04 | 内蒙古民族大学 | A kind of charcoal base dissolving phosphor and dissolving potassium bacterial manure and preparation method thereof |
| CN107278590A (en) * | 2017-07-28 | 2017-10-24 | 周运德 | Improve ginsenoside Rb in cultivation field seven1The method of content |
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Application publication date: 20180323 |