CN107771453A - A kind of method for improving Carrot Seed germination using gibberellin and concussion processing - Google Patents
A kind of method for improving Carrot Seed germination using gibberellin and concussion processing Download PDFInfo
- Publication number
- CN107771453A CN107771453A CN201610789046.5A CN201610789046A CN107771453A CN 107771453 A CN107771453 A CN 107771453A CN 201610789046 A CN201610789046 A CN 201610789046A CN 107771453 A CN107771453 A CN 107771453A
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- China
- Prior art keywords
- seed
- carrot
- carrot seed
- gibberellin
- germination
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
It the present invention relates to the use of gibberellin (GA3) and shake processing Carrot Seed, the method for promoting Carrot Seed to sprout, this method can improve the germination rate of Carrot Seed.This method includes：Carrot Seed preprocess method, reagent treatment concentration and its processing time, seed type of seeding, seed culture condition etc..
Description
Technical field
The present invention relates to one kind using Gibberellins solution processing Carrot Seed, handled in combination with concussion, improve carrot
The method of germination.This method mainly includes：Carrot Seed preprocess method, reagent treatment Gibberellins solution concentration, processing
Time, concussion processing, seed type of seeding, seed culture condition etc., the proportioning of various technical parameters and medicament.
Background technology
Carrot Seed is small, its crust keratin, slow and irregular containing volatile oil, water penetration extreme difference, germination, to carrot
Production causes large effect.At present, promoting the method for plant seed germination has：HORMONE TREATMENT, intermittent warming, low-temperature treatment
The methods of, wherein being to promote one of easy-to-use mode of being germinateed in plant using HORMONE TREATMENT vegetable seeds.Therefore, we are more
On the basis of year is to carrot genetic breeding, research improves Carrot Seed germination rate and simultaneously shortens the germination cycle of Carrot Seed
Method is, and it is an object of the present invention to provide a kind of method for improving carrot germination rate and shortening the carrot germination cycle.
Gibberellin is a kind of important plant hormone, it participate in seed sprout, bloom, Sex Determination and leaf and fruit
The growth and development processes such as aging.Research shows that gibberellin mainly by promoting cell elongation mediate plant growth, say by follow-up study
Bright gibberellin can also control plant strain growth by adjusting cell quantity.GA3It is that active highest is a kind of in gibberellin class material, it is more
Apply for external source, plant strain growth can be promoted, germinate, yield positive results, fruit growth can be stimulated, improve setting percentage etc..
We have discovered that gibberellin can promote the germination of Carrot Seed, to the life of carrot somatic embryogenesis fetal hair extremely
It is important, and it plays a significant role in the elongation of carrot stem, the growth of root and starting of flower etc..Due to carrot seed
Son is small, rich in inhibiting substances, early growth is slow the characteristics of, this method first carries out immersion treatment to Carrot Seed, using certain dense
Spend GA3Soak simultaneously oscillation treatment, then sowed in 9cm culture dishes, observation and statistics germination.Technology master
It is related to：Carrot Seed preprocess method, reagent treatment concentration and its processing time, seed type of seeding, seed culture bar
Part etc., the proportioning of various technical parameters and medicament.
The content of the invention
It is a kind of using finite concentration gibberellin processing Carrot Seed present invention aims at providing, improve Carrot Seed hair
The method of bud.
The present invention by Carrot Seed preprocess method, reagent treatment concentration and its processing time, seed type of seeding,
Seed culture condition etc., the proportioning of various technical parameters and medicament etc..Realize and improve Carrot Seed germination percentage and shortening carrot
The time required to germination.
Brief description of the drawings
Fig. 1 carrot seeding situations.
The different development number of days germination number germination processes of Fig. 2 Carrot Seed.
Fig. 3 various concentrations GA3 handles the germination rate (6d statistical results) of Carrot Seed.
Fig. 4 various concentrations GA3 handles the root activity of Carrot Seed 10d seedlings, and root activity is that tetrazole reduction is strong
Degree.
Fig. 5 various concentrations GA3 handles the chlorophyll content of Carrot Seed seedling.
Embodiment
1. Carrot Seed preprocess method, reagent treatment concentration and its processing time.Predominantly at seed immersion and vibration
Reason.Carrot Seed is small, and seed coat is coarse barbed, prevents neutron absorption moisture, while carrot contains Germination Inhibitors, shadow
Ring the germination of carrot.Oscillation treatment purpose is to make mortifier contained by seed to spin off, and promotes seed water suction, and separation
Debris and cleaning seed.Using GA3Handled, working concentration is respectively 0,50,100,200,250,300,350mg/L, shake
Swing and carried out using oscillator, 1200 turns every point so that germination inhibiting substances are oozed out.About 2h is shaken, liquid in pipe is removed, adds
Enter corresponding solution to continue to shake about 2h.
2. seed type of seeding.After the completion of processing, treatment fluid is removed, is then cleaned with clear water, washing distilled water is
It is colourless therefore, remove moisture.Seed is inoculated in the culture dish that preprepared has completed filter paper, culture dish is diameter
9cm, a height of 2cm, grown beneficial to carrot seedling, application rate is about 90~100 seeds of each culture dish, the culture dish being inoculated with
Use ParafilmTM (Fig. 1) in time.
3. seed culture.Carrot Seed be light seed, it is necessary to certain light intensity and light application time, at a temperature of, ability
Preferably sprout.The carrot that will have been sowed, it is 300 μm of ol (m to be positioned over optical density2·s) -1, photoperiod 12h/12h
(illumination/dark), temperature are trained in case for the light of 22 DEG C/18 DEG C (illumination/dark).Whether detection seed goes mouldy daily, and time shift
Except mouldy seed.
Result of the test：
As a result one：By vibration processing, the inhibiting substances in removable Carrot Seed, GA3Carrot ' the black field five of immersion
It is very little ' germination shifts to an earlier date, and Carrot Seed about 2d starts to show money or valuables one carries unintentionally, and 8d cotyledons deploy entirely.The Carrot Seed about 3d soaked by water
Show money or valuables one carries unintentionally, in addition, seedling root growth situation is compared with GA3' black five cun of field ' seed seedling of immersion is poor (Fig. 2).
As a result two：By various concentrations GA3Carrot ' black five cun of field ' seed germination rate of immersion has differences, and passes through
200、250mg/L GA3The Carrot Seed germination rate highest soaked, and pass through 100,300mg/L GA3The carrot soaked
Seed germination rate takes second place (Fig. 3).
As a result three：Take each concentration processing growth 10d carrot seedling root system to carry out root activity measure, find warp
250mg/L GA3The root activity (tetrazole reduction intensity) of carrot ' black five cun of the field ' seedling soaked is most strong, 100,
350mg/L GA3The root activity of carrot ' black five cun of field ' seedling of immersion takes second place (Fig. 4).
As a result four：Each concentration processing growth 10d carrot seed seedling leaf measure chlorophyll content is taken, is found through 300mg/L
GA3Carrot ' black five cun of field ' the seedling chlorophyll content in leaf blades highest soaked, and 150,200,250mg/L GA3Soaked
Carrot ' black five cun of field ' seedling chlorophyll content in leaf blades takes second place (Fig. 5).Consider, select 200-250mg/L GA3Immersion is recklessly
Radish ' black five cun of field ' Carrot Seed, can improve its germination rate and viability.
Claims (4)
1. in Carrot Seed processing procedure, the proportioning of various technical parameter and gibberellin medicaments.
2. the Carrot Seed preprocess method that this patent is related to, reagent treatment concentration and its processing time, seed type of seeding,
Seed culture condition etc., the proportioning of various technical parameters and medicament.
3. purposes according to claim 1, it is characterised in that profit of the processing method in Carrot Seed germination process
With.
4. according to claim 2, material used in the processing method implementation process, the compounding medicine being related to and each
Kind technical parameter etc., the utilization in Carrot Seed germination process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610789046.5A CN107771453A (en) | 2016-08-25 | 2016-08-25 | A kind of method for improving Carrot Seed germination using gibberellin and concussion processing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610789046.5A CN107771453A (en) | 2016-08-25 | 2016-08-25 | A kind of method for improving Carrot Seed germination using gibberellin and concussion processing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107771453A true CN107771453A (en) | 2018-03-09 |
Family
ID=61451176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610789046.5A Pending CN107771453A (en) | 2016-08-25 | 2016-08-25 | A kind of method for improving Carrot Seed germination using gibberellin and concussion processing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107771453A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040200144A1 (en) * | 2003-03-17 | 2004-10-14 | Dainichiseika Color & Chemicals Mfg. Co., Ltd. | Plant growth regulator and a method of producing the regulator |
-
2016
- 2016-08-25 CN CN201610789046.5A patent/CN107771453A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040200144A1 (en) * | 2003-03-17 | 2004-10-14 | Dainichiseika Color & Chemicals Mfg. Co., Ltd. | Plant growth regulator and a method of producing the regulator |
Non-Patent Citations (2)
| Title |
|---|
| 王三根: "《蔬菜调控与保鲜实用技术》", 31 December 2007, 金盾出版社 * |
| 黄于明等: "《蔬菜种子工作手册》", 31 December 1993, 上海科学技术出版社 * |
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| PB01 | Publication | ||
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Application publication date: 20180309 |