CN107764881A - A kind of portable blood test kit device based on electrochemical method - Google Patents
A kind of portable blood test kit device based on electrochemical method Download PDFInfo
- Publication number
- CN107764881A CN107764881A CN201711208119.8A CN201711208119A CN107764881A CN 107764881 A CN107764881 A CN 107764881A CN 201711208119 A CN201711208119 A CN 201711208119A CN 107764881 A CN107764881 A CN 107764881A
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- Prior art keywords
- reagent
- blood
- device based
- electrochemical method
- test kit
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000002848 electrochemical method Methods 0.000 title claims abstract description 17
- 238000009534 blood test Methods 0.000 title claims description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 120
- 239000008280 blood Substances 0.000 claims abstract description 55
- 210000004369 blood Anatomy 0.000 claims abstract description 55
- 238000006243 chemical reaction Methods 0.000 claims abstract description 18
- 238000001514 detection method Methods 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 7
- 230000008676 import Effects 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 8
- 238000001035 drying Methods 0.000 abstract description 3
- 230000004888 barrier function Effects 0.000 abstract 1
- 238000010276 construction Methods 0.000 abstract 1
- 230000008569 process Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 3
- 239000006258 conductive agent Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000003487 electrochemical reaction Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
Abstract
The present invention relates to the content of something in detection blood, can effectively solve precision, high technical barrier is required to HCT, its solution is that caused current signal during the liquid reagent and reaction of blood in this kit device is detected using the method for electrochemical method, and the current signal detected is converted into the concentration of something by detector.The present invention, which is one, includes electrode, reagent bag, reagent reacting pond and the portable reagent box device of blood collection region, and reagent bag can detect the reaction reagent of the material according to the different dresses of material in detection blood.Simple in construction, novel and unique of the invention, precision is high, and the overstocked size of haemocyte of blood is not required, easy to use, can effectively solve not high and high to the HCT size requirements problem of prior art precision as caused by the test paper of photolorimetry and dry process.
Description
Technical field
The invention belongs to medical science in-vitro diagnosis field, and in particular to a kind of based on electrochemical method, more available for people
The portable kit device of kind blood testing project.
Background technology
Blood testing at present turns into clinical patient and the common detection methods of Healthy People physical examination, present blood testing
Have：1st, something content in blood is detected on semi-automatic or automatic clinical chemistry analyzer using the principle of photolorimetry；2nd, it is sharp
With something content in photochemical method or electrochemical process test paper detection blood.
Semi-automatic and automatic clinical chemistry analyzer volume current first is big, it is impossible to carries whenever and wherever possible, it is necessary to arrive hospital
Or clinic carries out blood testing, and it is long to detect required time, it is impossible to quickly goes out result；Secondly photolorimetry using light source and shows
Color substance detects to the amount of contained substance in blood, and such method is compared to electrochemical process, than electrochemical process precision
It is low with sensitivity；Again, if carrying out the detection of content of material in blood with test paper easy to carry, even if being entered with electrochemical process
Row detection, reagent carry out dry process on test paper, are so likely to result in and cause blood and reagent to mix uneven, detection knot
Fruit may be inaccurate；The test paper of dry process overstocks (HCT) to the haemocyte in blood certain requirement, if blood is not up to
To this requirement, testing result can be caused inaccurate.
The content of the invention
In view of this, the present invention propose it is a kind of can Portable belt, it is based on electrochemical method, blood testing project liquid can be filled
The kit device of body reagent.Can effectively solve detection time length, detection is inconvenient, and precision and sensitivity are low, will to HCT
Ask the problems such as high.
To reach above-mentioned purpose, the technical proposal of the invention is realized in this way：
A kind of portable blood test kit device based on electrochemical method, including electrode, reagent bag, reagent reacting
Pond and blood collection region；The reagent bag is closed container, and lower section is provided with the Mini-sized manual valve being connected with reagent reacting pond；It is described to adopt
Blood area is connected with reagent reacting pond by interface, and it is anti-that by the interface between reagent reacting pond blood sample is entered into reagent
Ying Chizhong；The electrode is arranged in the reagent reacting pond, is connected for caused electric current in detection reaction and with instrument, is incited somebody to action
Electric current is transferred on instrument detecting element in the reaction detected.
Further, the electrode is electrode group, including at least 2 electrodes, i.e. working electrode and reference electrode；Or it is
Multiple electrodes.
Further, described electrode group one end is located at reagent reacting pond lower right side, and the other end is used to be connected with instrument.
Further, the electrode group is separately formed, is connected again with reagent reacting pond, instrument during use；The reagent
Reaction tank, instrument are respectively equipped with electrode interface.
Further, the reagent bag is the conjuncted closed container being linked togather of two or more, each reagent bag
There is the Mini-sized manual valve being connected with reagent reacting pond lower section.
Further, the Mini-sized manual valve is provided with manual opening stem, and manual opening stem is located inside reagent bag, is connected to
The Mini-sized manual valve and reagent bag top；The manual opening stem installation site has mark outside reagent bag.
Further, the blood collection region is the siphon pipe that finger tip blood or venous blood are drawn by siphonage, the rainbow
Suction pipe is connected with reagent reacting pond by interface.
Further, the interface is miniature suction pipe, according to the liquid that can allow calculated in the presence of atmospheric pressure
The critical bore of only import but no export, as the bore of miniature suction pipe, ensure that blood can be into reagent reacting pond and in reaction tank
Liquid reagent will not flow out.
Further, the reagent reacting pond is the rectangle box body with biodegradable environmental-protecting material, the reagent
Bag is above box body.
Relative to prior art, a kind of portable blood detection reagent based on electrochemical method of the present invention is box-packed
Put and have the advantage that：
The present invention be directed to the novel portable electrochemical blood test reagent designed the defects of prior art is box-packed
Put, can not only detect content of material different in blood, have more with property, also solve large-scale semi-automatic and full-automatic
Biochemical Analyzer detection time is grown, and some not high defects of accuracy of photolorimetry, while also overcomes by dry type
The test paper of reason mixes problem uneven, high to HCT requirements to reagent with blood.
The present invention compared with prior art, has the characteristics of following prominent：
1st, precision is high, and something content is more much higher than photolorimetry precision in electrochemical process measure blood, the present invention
Reagent be liquid reagent, be well mixed with blood, accuracy of measurement is high；
2nd, overcome and high problem is required to the haemocyte overstocked (HCT) of blood；
3rd, detection time is short；
4th, small volume, it is in light weight, it is easy to carry, blood testing can be carried out whenever and wherever possible.
Brief description of the drawings
The portable blood test kit device based on electrochemical method that Fig. 1 is the present invention is shown (by taking three reagents as an example)
It is intended to.
Wherein：
1st, electrode；2nd, reagent bag；3rd, reagent reacting pond；
4th, blood collection region；5th, Mini-sized manual valve；6th, miniature suction pipe；
7th, manual opening stem.
Embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
Describe the present invention in detail below with reference to the accompanying drawings and in conjunction with the embodiments.
As shown in Figure 1, the present invention includes electrode 1, reagent bag 2, reagent reacting pond 3 and blood collection region 4；
Wherein at least 2 electrodes (working electrode and reference electrode) of electrode 1, it is possibility to have multiple electrodes, such as 6-8
Electrode, form electrode group；Described electrode group one end is located at reagent reacting pond lower right side, and the other end is used to be connected with instrument.Or
The electrode group is separately formed, is connected again with reagent reacting pond, instrument during use；The reagent reacting pond, instrument are set respectively
There is electrode interface.
For reagent bag 2, if single reagent, then an only closed autonomous container is as reagent bag 2, reagent bag 2
Lower section is provided with the Mini-sized manual valve 5 being connected with reagent reacting pond 3；
If double reagent either more reagents, then there are 2 or multiple conjuncted closed containers as reagent bag 2, specifically
The number of closed container is determined by different detection project reaction principles in reagent bag 2, and each lower section of reagent bag 2 has
The Mini-sized manual valve 5 being connected with reagent reacting pond 3；
Want to include reagent in reaction tank in said structure, by the SOLUTION PROPERTIES and design requirement concrete decision of reality.
One or more Mini-sized manual valve 5 between described reagent bag 2 and reagent reacting pond 3 is a kind of drive manually
Movable valve, it is that manually valve rod 7 controls its on off state, and manual opening stem 7 is connected to Mini-sized manual valve 5 and reagent bag
2 tops, the manual opening stem installation site inside reagent bag 2 have mark outside reagent bag.When carrying out blood testing, every
Certain pressure, the hand-operated valve of valve rod connection are downwardly applied on the mark of manual opening stem 7 directly over individual reagent bag 2 with finger vertical
Door is opened, and when pressure does not have, valve is closed.The valve connects each reagent bag and reagent reacting pond respectively, when valve is opened,
The reagent in each reagent bag 2 is set to enter reagent reacting pond 3.
Reagent reacting pond 3 carries out electrochemical reaction appts after being mixed for differential responses reagent with blood；When blood and examination
It is reverse manually to mix after agent enters reagent reacting pond 3, reagent and blood is carried out sufficient electrochemical reaction.Reagent reacting pond 3
The reagent bag of one of which reagent is also acted as, i.e., when detection reagent is single reagent, reagent reacting pond 3 is equipped with reagent
Reagent bag；When detection reagent is double reagent or more reagents, reagent reacting pond 3 is the reagent bag for filling one of which reagent.
Its structure is that top has one or more Mini-sized manual valve 5, for connecting each reagent bag 2.
Blood collection region 4 is siphon pipe, using siphonage draw blood sample, described blood collection region 4 and the phase of reagent reacting pond 3
For interface even in the upper left side in reagent reacting pond 3, the interface is that calculating one can allow according in the presence of atmospheric pressure
This principle of the critical bore of liquid only import but no export and the miniature suction pipe 6 designed, the miniature bore of suction pipe 6 are less than 0.5cm.
The miniature suction pipe can ensure that blood can enter reagent reacting pond and the liquid reagent in reaction tank will not flow out.
Kit device shown in Fig. 1 is connected by electrode with instrument, connection reagent reacting pond 3 and different reagent bags
Mini-sized manual valve 5 manually open by valve rod 7, so that different reagents enters in reagent reacting pond 3, blood collection region 4 utilizes
The venous blood that finger tip blood or vein hemostix that blood taking needle is taken extract is entered reagent by siphonage by miniature suction pipe 6
It is reverse manually to be well mixed reagent and blood in reaction tank 3, something in blood is sufficiently aoxidized with reaction reagent
Reduction reaction, the reaction produce electronics e-, caused electronics e- and are reduced conductive agent in reagent mixed liquor from high price (such as 3+)
To at a low price (such as 2+), then instrument gives reaction reagent some specific voltage by electrode, and conductive agent is again from (such as 2+) at a low price by oxygen
Chemical conversion high price (such as 3+), so as to discharge electronics e-, electrode detection to the current signal, is transferred to instrument detecting element, then
Instrument is detected to current signal and is converted into concentration value, and the content of the material in blood is calculated so as to analyze.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (9)
1. a kind of portable blood test kit device based on electrochemical method, it is characterised in that including electrode, reagent
Bag, reagent reacting pond and blood collection region；The reagent bag is closed container, and lower section is provided with the Mini-sized manual being connected with reagent reacting pond
Valve；The blood collection region is connected with reagent reacting pond by interface, and blood sample is passed through into the interface between reagent reacting pond
Enter in reagent reacting pond；The electrode is arranged in the reagent reacting pond, for caused electric current in detection reaction simultaneously
It is connected with instrument, electric current in the reaction detected is transferred on instrument detecting element.
2. a kind of portable blood test kit device based on electrochemical method according to claim 1, its feature
It is, the electrode is electrode group, including at least 2 electrodes, i.e. working electrode and reference electrode；Or it is multiple electrodes.
3. a kind of portable blood test kit device based on electrochemical method according to claim 2, its feature
It is, described electrode group one end is located at reagent reacting pond lower right side, and the other end is used to be connected with instrument.
4. a kind of portable blood test kit device based on electrochemical method according to claim 2, its feature
It is, the electrode group is separately formed, is connected again with reagent reacting pond, instrument during use；The reagent reacting pond, instrument point
She You not electrode interface.
5. a kind of portable blood test kit device based on electrochemical method according to claim 1, its feature
It is, the reagent bag is the conjuncted closed container being linked togather of two or more, has and tries below each reagent bag
The connected Mini-sized manual valve of agent reaction tank.
6. a kind of portable blood test kit device based on electrochemical method according to claim 1 or 5, it is special
Sign is that the Mini-sized manual valve is provided with manual opening stem, and manual opening stem is located inside reagent bag, is connected to the Mini-sized manual
Valve and reagent bag top；The manual opening stem installation site has mark outside reagent bag.
7. a kind of portable blood test kit device based on electrochemical method according to claim 1, its feature
It is, the blood collection region is the siphon pipe that finger tip blood or venous blood are drawn by siphonage, and the siphon pipe and reagent are anti-
Ying Chi is connected by interface.
8. a kind of portable blood test kit device based on electrochemical method according to claim 1 or 7, it is special
Sign is that the interface is miniature suction pipe, and liquid only import but no export can be allowed according to what is calculated in the presence of atmospheric pressure
Critical bore, as the bore of miniature suction pipe, the liquid reagent that guarantee blood can be into reagent reacting pond and in reaction tank is not
It can flow out.
9. a kind of portable blood test kit device based on electrochemical method according to claim 1, its feature
It is, the reagent reacting pond is the rectangle box body with biodegradable environmental-protecting material, and the reagent bag is located on box body
Side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711208119.8A CN107764881A (en) | 2017-11-27 | 2017-11-27 | A kind of portable blood test kit device based on electrochemical method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711208119.8A CN107764881A (en) | 2017-11-27 | 2017-11-27 | A kind of portable blood test kit device based on electrochemical method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107764881A true CN107764881A (en) | 2018-03-06 |
Family
ID=61276984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711208119.8A Pending CN107764881A (en) | 2017-11-27 | 2017-11-27 | A kind of portable blood test kit device based on electrochemical method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107764881A (en) |
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