A kind of essence that moisturizing is releived dew and preparation method thereof
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of essence that moisturizing is releived dew and preparation method thereof.
Background technique
The skin of people divides epidermis, corium and subcutaneous fat.The outermost layer of epidermis is cuticula, due to cuticula itself
The work of natural moisturizing factor, that is, amino acids, lactate and carbohydrate contained in water suction, barrier function and cuticula etc.
With making cuticula keep certain water content, maintain the wet of skin.And the appearance of skin and the moisture content of cuticula have
It closes, normal keratoderma usually contains the moisture of 10%-30%, to maintain the softness and elasticity of skin.But with the age
Growth, keratoderma moisture content can gradually decrease, and when the moisture content of keratoderma is lower than 10%, and skin is just
It will appear dry, tight, coarse and furfur etc..In general, cause the unbalanced internal and external condition of skin natural moisture preserving structure
Mainly have: 1, as the increasing at age, skin aging, moisture-keeping function and barrier function gradually weaken, natural moisturizing factor content
It reduces.2, dry cold weather, humidity is lower, such as autumn and winter.3, certain cutaneous lesions, such as psoriasis, ichthyosis 4, ring
The organic solvents such as border and chemical factor, such as washing powder, soap, dish washing liquid detergent and wine and prolonged air conditioner surroundings
Deng.5, diet sleep habit, such as partial eclipse, drinking-water less, insomnia.Above-mentioned various reasons cause the skin of people to occur water shortage for a long time
Symptom, and then cause more skin problems, such as long spot, small pox, wrinkle, relaxation, therefore be most basic to skin moisture-keeping
Skin care step.
It a kind of is of great significance with the releive cosmetics of effect of moisturizing in conclusion researching and developing.
Summary of the invention
For these reasons, applicant has found a kind of new cosmetics, contains in the cosmetics containing height by studying for many years
The purslane extract of amount, to guarantee that there is cosmetics moisturizing to releive effect after being combined with other compositions, studies have shown that this hair
Bright cosmetics have good stability, have good moisture-keeping function for Skin Cell.
What the present invention was achieved through the following technical solutions.
A kind of moisturizing is releived essence dew, and essence dew is prepared by following raw materials: purslane extract, dried tremella extract
And glycine betaine.
Essence dew is prepared by following raw materials: purslane extract 15-20 parts by weight, 0.001 weight of dried tremella extract
Measure part, glycine betaine 0.4-0.6 parts by weight.
Essence dew is prepared by following raw materials: purslane extract, dried tremella extract, glycine betaine, hydroxy ethyl fiber
Plain, polyacrylic acid grafted starch, EDTA- disodium, tocopherol, trehalose, Sodium Hyaluronate, dipotassium glycyrrhizinate, beta glucan,
Silver.
Essence dew is prepared by following raw materials: water, hydroxyethyl cellulose, polyacrylic acid grafted starch, glycerol, 1,3-
Butanediol, glycine betaine, isoprene, 1,2- hexylene glycol, EDTA- disodium, Cremophor RH40, olive oil PEG-7 esters,
Tocopherol, octyl dodecanol, dried tremella extract, purslane extract, trehalose, Sodium Hyaluronate, dipotassium glycyrrhizinate, β-Portugal are poly-
Sugar, silver.
Essence dew is prepared by following raw materials: water 65-70 parts by weight, are gathered at hydroxyethyl cellulose 0.2-0.4 parts by weight
Acrylic acid grafted starch 0.10-0.20 parts by weight, glycerol 2-4 parts by weight, 1,3-BDO 5-8 parts by weight, glycine betaine 0.4-0.6
Parts by weight, isoprene 1.5-2.5 parts by weight, 1,2- hexylene glycol 0.4-0.6 parts by weight, EDTA- disodium 0.04-0.06 weight
Part, Cremophor RH40 0.25-0.35 parts by weight, olive oil PEG-7 esters 0.25-0.35 parts by weight, tocopherol 0.08-
0.12 parts by weight, octyl dodecanol 0.08-0.12 parts by weight, 0.001 parts by weight of dried tremella extract, purslane extract 15-20
Parts by weight, trehalose 0.08-0.12 parts by weight, Sodium Hyaluronate 0.004-0.006 parts by weight, dipotassium glycyrrhizinate 0.04-0.06
Parts by weight, beta glucan 0.05-0.15 parts by weight, silver 0.1-0.3 parts by weight.
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
A kind of moisturizing is releived essence dew, the application being in cosmetics that skin moisture-keeping, skin are releived.
Raw material of the present invention is purchased from and is shown in Table 1:
The raw material sources of the present invention of table 1
Raw material |
Purchased from producer |
Date of manufacture |
Hydroxyethyl cellulose |
U.S. Ya Shilan |
2016.04.03 |
Polyacrylic acid grafted starch |
Daito Kasei Kogyo |
2015.12.06 |
Glycerol |
Cocoanut tree Acidchem |
2016.08.09 |
1,3-BDO |
Japan DAICEL |
2017.01.08 |
Glycine betaine |
Asahi Chemical Industry Asahi Kasel |
2016.04.03 |
Isoprene |
Japan Kuraray |
2016.05.10 |
1,2- hexylene glycol |
Symrise |
2016.02.18 |
EDTA- disodium |
Aksu AKZO NOBEL |
2016.01.19 |
Cremophor RH40 |
German BASF |
2016.11.23 |
Olive oil PEG-7 esters |
Taiwan CORUM |
2015.12.24 |
Tocopherol |
Zhejiang YIYAO |
2017.03.05 |
Purslane extract |
Guangzhou standing grain base |
2016.09.08 |
Trehalose |
Japanese woods is former |
2016.05.04 |
Sodium Hyaluronate |
The prosperous Fu Ruida of China |
2017.02.23 |
Dipotassium glycyrrhizinate |
Gansu Qinghai Lake medicine company |
2016.10.21 |
Beta glucan |
Korea Macrocare Tech |
2015.11.29 |
Silver |
The Japan longevity chamber of commerce |
2017.05.08 |
Specific embodiment
Below the technical scheme of the invention is illustrated by a specific example, but the scope of the present invention is not limited thereto.
Content described in this specification embodiment is only enumerating to the way of realization of inventive concept, protection of the invention
Range should not be construed as being limited to the specific forms stated in the embodiments, and protection scope of the present invention is also and in art technology
Personnel conceive according to the present invention it is conceivable that equivalent technologies mean.Although embodiment of the invention below is retouched
It states, but the invention is not limited to above-mentioned specific embodiments and applications field, following specific embodiments is only to show
It is meaning property, guiding, rather than it is restrictive.Those skilled in the art under the enlightenment of this specification and are not taking off
In the case where the range protected from the claims in the present invention, a variety of forms can also be made, these belong to the present invention
The column of protection.
The following tests of the present invention are on the basis of multiple creative test, with the claimed technical solution of the present invention
Based on, the conclusive test of the research staff of summary.
Test 1
Moisturizing experimental study
Test 1 group: water 67mg, hydroxyethyl cellulose 0.3mg, polyacrylic acid grafted starch 0.15mg, glycerol 3mg, 1,3-
Butanediol 6.5mg, glycine betaine 0.5mg, isoprene 2.0mg, 1,2- hexylene glycol 0.5mg, EDTA- disodium 0.05mg, PEG-40
Rilanit special 0.30mg, olive oil PEG-7 esters 0.30mg, tocopherol 0.10mg, octyl dodecanol 0.10mg, tremella extract
Object 0.001mg, purslane extract 17.5mg, trehalose 0.10mg, Sodium Hyaluronate 0.005mg, dipotassium glycyrrhizinate 0.05mg,
Beta glucan 0.30mg, silver 0.20mg.
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
Test 2 groups: water 67mg, hydroxyethyl cellulose 0.3mg, polyacrylic acid grafted starch 0.15mg, glycerol 3mg, 1,3-
Butanediol 6.5mg, glycine betaine 0.5mg, isoprene 2.0mg, 1,2- hexylene glycol 0.5mg, EDTA- disodium 0.05mg, PEG-40
Rilanit special 0.30mg, olive oil PEG-7 esters 0.30mg, tocopherol 0.10mg, octyl dodecanol 0.10mg, tremella extract
Object 0.001mg, purslane extract 0.005mg, trehalose 0.10mg, Sodium Hyaluronate 17.5mg, dipotassium glycyrrhizinate 0.05mg,
Beta glucan 0.30mg, silver 0.20mg.
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
Test method: Sodium Hyaluronate (cosmetics-stage);Ammonium sulfate and discoloration silica gel;MTT;Cell culture medium: contain
The MEM culture medium of 10% calf serum (PAA), 100U/ml penicillin and 100U/ml streptomysin;0.25% pancreatin;Cell people's table
Cornu cutaneum cell plastid strain HaCat;
When epidermal growth to fusion rate is up to 80% or more, the MEM culture medium without calf serum is added and synchronizes
Change processing 12h, collected by trypsinisation adjusts cell concentration after counting, is seeded to 24 well culture plates.After cell is adherent, it is added
Prepared 1000 μ g/ml of test group cosmetics is diluted with culture medium in advance, is incubated for, positive control is Sodium Hyaluronate
(1000μg/ml).After for 24 hours, it is placed in super-clean bench under certain wind speed after being dried, fresh culture is added, continues to train
It supports for 24 hours, carries out MTT measurement.Using without any processing group as blank control group in experiment.It is dry that cell is calculated as follows
The death rate (%) and protection ratio (%).
The cell dry death rate (%)=(1- detection group OD value/normal group OD value) × 100%
Protection ratio (%)=(the dry death rate of the dry death rate-test group cell of blank group cell)/blank group cell is dry
The dry death rate × 100%
Test result: it is shown in Table 1.
The comparison of 1 each group epidermal cell of the table dry death rate and protection ratio
Note: P < 0.05 * * P < 0.01, * compared with blank group.
Conclusion (of pressure testing): it is found that positive controls are shown preferably carefully to epidermal cell dry-run protection experimental study
Born of the same parents' protective effect, and test group of the present invention also has good cytoprotection, purslane extract is substituted for same
Sodium Hyaluronate (2 groups of test), declines cytoprotection, further proves, essence dew of the present invention can significantly be resisted dry
The dry damage to epidermal cell reduces the dry influence to cell viability, has good moisture-keeping function.
Test 2
Heat resistant test
Test 1 group: water 67mg, hydroxyethyl cellulose 0.3mg, polyacrylic acid grafted starch 0.15mg, glycerol 3mg, 1,3-
Butanediol 6.5mg, glycine betaine 0.5mg, isoprene 2.0mg, 1,2- hexylene glycol 0.5mg, EDTA- disodium 0.05mg, PEG-40
Rilanit special 0.30mg, olive oil PEG-7 esters 0.30mg, tocopherol 0.10mg, octyl dodecanol 0.10mg, tremella extract
Object 0.001mg, purslane extract 17.5mg, trehalose 0.10mg, Sodium Hyaluronate 0.005mg, dipotassium glycyrrhizinate 0.05mg,
Beta glucan 0.30mg, silver 0.20mg.
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
Test 2 groups: water 67mg, hydroxyethyl cellulose 0.3mg, glycerol 3mg, 1,3-BDO 6.5mg, glycine betaine 0.5mg,
Isoprene 2.0mg, 1,2- hexylene glycol 0.5mg, EDTA- disodium 0.05mg, Cremophor RH40 0.30mg, olive oil
PEG-7 esters 0.30mg, tocopherol 0.10mg, octyl dodecanol 0.10mg, dried tremella extract 0.001mg, purslane extract
17.5mg, trehalose 0.10mg, Sodium Hyaluronate 0.005mg, dipotassium glycyrrhizinate 0.05mg, beta glucan 0.30mg, silver
0.20mg。
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, EDTA bis-
Sodium, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.Test method: by different essence
The plastic drug package of 2 bottles of clean dries is added in dew, tightens lid, and one bottle is put into and has been pre-adjusted the constant temperature that temperature is 48 ± 1 DEG C
It in case, is taken out after 48 hours, after recovery room temperature compared with another bottle carries out range estimation.
Test result: 3 are shown in Table.
3 heat resistant test result of table
Group |
Whether there is water-oil separating before test |
Whether there is water-oil separating after test |
Test 1 group |
It is no |
It is no |
Test 2 groups |
It is no |
It is |
Conclusion (of pressure testing): heat resistant test shows after polyacrylic acid grafted starch is added in essence dew of the present invention that essence dew has
Better stability.
Prepare embodiment
Embodiment 1
Essence reveals raw material are as follows: water 65g, hydroxyethyl cellulose 0.2g, polyacrylic acid grafted starch 0.10g, glycerol 2g, 1,3-
Butanediol 5g, glycine betaine 0.4g, isoprene 1.5g, 1,2- hexylene glycol 0.4g, EDTA- disodium 0.04g, PEG-40 hydrogenated castor
Oily 0.25g, olive oil PEG-7 esters 0.25g, tocopherol 0.08g, octyl dodecanol 0.08g, dried tremella extract 0.001g, horse
Sativa extract 15g, trehalose 0.08g, Sodium Hyaluronate 0.004g, dipotassium glycyrrhizinate 0.04g, beta glucan 0.30g, silver
0.20g。
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
Embodiment 2
Essence reveals raw material are as follows: water 70g, hydroxyethyl cellulose 0.4g, polyacrylic acid grafted starch 0.20g, glycerol 4g, 1,3-
Butanediol 8g, glycine betaine 0.6g, isoprene 2.5g, 1,2- hexylene glycol 0.6g, EDTA- disodium 0.06g, PEG-40 hydrogenated castor
Oily 0.35g, olive oil PEG-7 esters 0.35g, tocopherol 0.12g, octyl dodecanol 0.12g, dried tremella extract 0.001g, horse
Sativa extract 20g, trehalose 0.12g, Sodium Hyaluronate 0.006g, dipotassium glycyrrhizinate 0.06g, beta glucan 0.30g, silver
0.20g。
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
Embodiment 3
Essence reveal raw material are as follows: water 66g, hydroxyethyl cellulose 0.25g, polyacrylic acid grafted starch 0.12g, glycerol 2.5g,
1,3-BDO 5.5g, glycine betaine 0.45g, isoprene 1.6g, 1,2- hexylene glycol 0.45g, EDTA- disodium 0.05g, PEG-40
Rilanit special 0.28g, olive oil PEG-7 esters 0.28g, tocopherol 0.09g, octyl dodecanol 0.09g, dried tremella extract
0.001g, purslane extract 18g, trehalose 0.09g, Sodium Hyaluronate 0.005g, dipotassium glycyrrhizinate 0.045g, beta glucan
0.30g, silver 0.20g.
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
Embodiment 4
Essence reveals raw material are as follows: water 67g, hydroxyethyl cellulose 0.3g, polyacrylic acid grafted starch 0.15g, glycerol 3g, 1,3-
Butanediol 6.5g, glycine betaine 0.5g, isoprene 2.0g, 1,2- hexylene glycol 0.5g, EDTA- disodium 0.05g, PEG-40 hydrogenate castor
Sesame oil 0.30g, olive oil PEG-7 esters 0.30g, tocopherol 0.10g, octyl dodecanol 0.10g, dried tremella extract 0.001g,
Purslane extract 17.5g, trehalose 0.10g, Sodium Hyaluronate 0.005g, dipotassium glycyrrhizinate 0.05g, beta glucan 0.30g,
Silver-colored 0.20g.
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
Embodiment 5
Essence reveal raw material are as follows: water 68g, hydroxyethyl cellulose 0.35g, polyacrylic acid grafted starch 0.18g, glycerol 3.5g,
1,3-BDO 7g, glycine betaine 0.55g, isoprene 2.4g, 1,2- hexylene glycol 0.55g, EDTA- disodium 0.05g, PEG-40 hydrogen
Change castor oil 0.32g, olive oil PEG-7 esters 0.32g, tocopherol 0.11g, octyl dodecanol 0.11g, dried tremella extract
0.001g, purslane extract 18g, trehalose 0.11g, Sodium Hyaluronate 0.005g, dipotassium glycyrrhizinate 0.05g, beta glucan
0.30g, silver 0.20g.
The essence dew the preparation method comprises the following steps:
Water intaking, glycerol, isoprene, 1,3-BDO, glycine betaine, 1,2- hexylene glycol, hydroxyethyl cellulose, polyacrylic acid
Graft starch, EDETATE SODIUM, trehalose are added water phase pot, after being heated to 80-85 DEG C, stir evenly, and are pumped into agitated kettle;
Booting is uniformly mixed, and 30 minutes, 1000rpm/ points, is stopped stirring, is cooled to 40 DEG C;PEG-40 hydrogenation is added
Castor oil, olive oil PEG-7 esters, tocopherol, octyl dodecanol, dried tremella extract, purslane extract, Sodium Hyaluronate,
Dipotassium glycyrrhizinate, beta glucan, silver continue to stir evenly, and are cooled to room temperature to get essence dew.
Stability test research
Test 1: different essence: being revealed the plastic drug package that 2 bottles of clean dries are added, tightens lid by heat resistant test, and one bottle
Being put into and being pre-adjusted temperature is to take out after 48 hours in 48 ± 1 DEG C of insulating box, restore after room temperature with another bottle of progress mesh
Survey is compared.
Test 2: different essence: being revealed the plastic drug package that 2 bottles of clean dries are added, tightens lid by low temperature resistant test, and one bottle
Being put into and being pre-adjusted temperature is to take out after 48 hours in -18 ± 1 DEG C of refrigerator, restore after room temperature with another bottle of progress mesh
Survey is compared.
Test 3: different essence: being revealed the plastic drug package that 2 bottles of clean dries are added, tightens lid by Light Resistance Test, and one bottle
It places and is exposed to the sun 30 days under sunlight.
Test result: 4 are shown in Table.
4 stability test result of table
Aforementioned stable experiments have shown that, essence of the present invention dew has good stability.
It is including but not limited to above-mentioned to prepare embodiment.