CN107752042A - A kind of preparation method of oyster active peptides - Google Patents
A kind of preparation method of oyster active peptides Download PDFInfo
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- CN107752042A CN107752042A CN201710897189.2A CN201710897189A CN107752042A CN 107752042 A CN107752042 A CN 107752042A CN 201710897189 A CN201710897189 A CN 201710897189A CN 107752042 A CN107752042 A CN 107752042A
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- 239000002002 slurry Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- DSNBHJFQCNUKMA-SCKDECHMSA-N thromboxane A2 Chemical compound OC(=O)CCC\C=C/C[C@@H]1[C@@H](/C=C/[C@@H](O)CCCCC)O[C@@H]2O[C@H]1C2 DSNBHJFQCNUKMA-SCKDECHMSA-N 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 230000009278 visceral effect Effects 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A kind of preparation method of oyster active peptides, the present invention relates to traditional Chinese medicine and technical field of biology.It is formed using fresh oyster, extra large intestines and Chinese medicine as raw material through broken homogenate, enzymolysis, centrifugation, sterilizing.Material after homogenate is adjusted into pH value 68, adds the proteolytic enzyme of weight of material 0.1 1%, is digested 36 hours at 50 60 degrees Celsius；Add the dusty yeast of weight of material 0.5% 1% defishying 12 hours between 30 50 degree Celsius；Then enzyme deactivation, centrifugation, oyster active peptides liquid must be mixed by sterilizing；Freeze-dried powder product, oral liquid product, capsule product can be prepared.Described proteolytic enzyme is combination more than one or both of neutral proteinase, trypsase, compound protease, flavor protease, papain.The oyster active peptides have tonifying primordial Qi, beneficial the five internal organs, deficient, beautifying face and moistering lotion of dispelling, kidney-reinforcing Yang-strengthening, antifatigue, antitumor, antiviral, the enhancing pharmacological activity such as immunity of organisms and promoting growth of cell.Process scientific and precise of the present invention, strong operability, is easy to industrialized production.
Description
Technical field
The present invention relates to traditional Chinese medicine and technical field of biology, has tonifying primordial Qi, beneficial the five internal organs, void of dispelling more particularly, to one kind
Damage, beautifying face and moistering lotion, kidney-reinforcing Yang-strengthening, antifatigue, antitumor, antiviral, enhancing immunity of organisms and promoting growth of cell act on male
Oyster active peptide and preparation method thereof.
Background technology
Oyster is commonly called as oyster, is commonly called as oyster, alias oyster is yellow, oyster.Shell-fish, lamellibranchiata, Ostreidae.Oyster meat fertilizer
Smooth, delicious flavour is nutritious, containing nutritions such as abundant protein, fat, calcium, phosphorus, iron, is known as " seabed ox
The laudatory title of milk ".In west, oyster is still described as " god grants evil spirit food ", and what Japanese then praised is " source of root ".There is " south in China
The oyster of side, the bear's paw in the north " is said.Every 10.9 grams of 100 grams of edible part protein, 1.5 grams of fat, 200 milligrams of potassium, sodium
462 milligrams, 131 milligrams of calcium, 65 milligrams of magnesium, 9.39 milligrams of zinc, 7.1 milligrams of iron, 11.5 milligrams of copper, 115 milligrams of phosphorus, selenium 86 is micro-
Gram, the microgram of dehydroretinol 7,1.4 milligrams of nicotinic acid, amount of iodine is much higher by milk and yolk, the height of zinc content, can be food it
Hat.Also contain the distinctive various active material of marine organisms and several amino acids in oyster.
Oyster is not only delicacies cuisines, and also has very high medical value.Ming Dynasty's Li Shizhen《Compendium of Materia Medica》Record："
Oyster meat, sweet temperature is nontoxic, governance wasting of cooking, and in tune, solves erysipelas, mends married woman's qi and blood, eaten raw with ginger vinegar, control dysphoria with smothery sensation after drinking, quench the thirst.
Maximum phase of an eclipse U.S. is processed, makes us thin skin, U.S. color." Modern medical experiment is also shown that the aqueous solution extracted from oyster meat is thin to tumour
Born of the same parents have inhibitory action.Japanese Scientists, which also found in extra large oyster meat, contains natural taurine, has reducing blood lipid, suppresses platelet aggregation
Collection, improve body immunity, the multiple functions such as enhance metabolism.Oyster extract has obvious suppression platelet aggregation,
TXA2 contents in the blood fat and blood of high fat of blood patient can be reduced, are advantageous to insulin secretion and utilization, and can makes malignant tumour thin
Born of the same parents strengthen radiation-sensitive, and it is grown have inhibitory action；Contained abundant Taurine has obvious liver protection in oyster
Choleretic effect, this is also the good medicine for preventing and treating pregnancy period functional condition in intrahepatic cholestasis；Contained abundant trace element and glycogen, to promoting tire
The growing of youngster, rescue pregnant woman anemia and beneficial to the antisecosis of pregnant woman；Oyster is the best food replenished the calcium again, it
It is phosphorous very abundant, due to needing the help of phosphorus when calcium is by body absorption, so being advantageous to the absorption of calcium；Oyster also contains vitamin
B12, this is lacking in general food, and the brill element in vitamin B12 is the indispensable material of prevention pernicious anaemia institute, because
And oyster has the function that to enliven hematopoiesis function；There are a variety of excellent amino acid, these ammonia in contained protein in oyster
Base acid has detoxication, can remove internal noxious material, and tarine therein has reduction cholesterolemia concentration again
Effect, therefore can prevention of arterial hardening.
One of food of first integration of drinking and medicinal herbs that oyster is announced as the Ministry of Public Health of China, contain abundant protein, ox
Sulfonic acid, vitamin isoreactivity material.Taurine contained therein is a kind of important cell-protecting, and the effect of its primary biological is
The stabilization and regulation osmotic balance of membrane structure can be safeguarded, there is protective effect to the lipid peroxidation of cell membrane.Grind
Study carefully the regulation for showing that taurine participates in glycometabolism, accelerate glycolysis；It can be combined with insulin receptor, promote musculature to grape
Sugar, the uptake and utilization of amino acid, accelerate glycolysis, increase gluconeogenesis；Strengthen myocardial contractive power, increase blood exports, together
When prevent myocardial damage；Liver can be protected, maintains locomitivity, anti-motion anoxic and fatigue.
Extra large intestines, scientific name Urechis uniconctus (Urechis unicinctus), popular name:Extra large intestines, extra large intestines, belong to echiuran
Door (Echiurioidea), Yi guiding principles (Echiurida), without pipe Yi mesh (Xenopneusta), thorn Yi sections (Urchidae).It is
A kind of mollusk of oval tubular, wriggles, from head to foot impulse- free robustness softly, light yellow.Extra large intestines not only look like nude sea cucumber,
Its nutritive value is also in no way inferior compared with sea cucumber.Extra large intestines have stronger tolerance and detoxification ability to sulfide, are also therefore taken as edge
The model organism of extra large sulfide metabolism correlative study.Extra large intestines are mainly distributed within the border in Shandong:The coastal mud in the ground such as Yantai, Qingdao
In beach or rock seam.Shandong Jiaodong District is the maximum place of production of China sea intestines.Its individual is loose, and meat flavour is delicious, and body wall flesh is rich in
Protein and a variety of essential amino acids.Since ancient times, it is coastal as rare seafood food in China, Japan and Korea
Product, there is higher economic value.The traditional method of people only eats its body wall, and discarded internal organ, dish are commonly called as extra large intestines.But have
Research finds, it is 18.25% that extra large intestines, which discard visceral protein matter content, fat content 0.12%, total sugar content be 4.09% and
Contain the elements such as abundant Ca, Mg, Fe, Zn;Also containing abundant EPA, DHA and DPA.Pharmaceutical research shows:Extra large intestines（It is single
Ring pierces Yi）The polypeptide of extraction plays the role of certain antitumor and raising immune function of mice.Also someone did extra large intestines（Monocyclic thorn
Yi）Antithrombotic component isolate and purify and pharmacodynamic study.Therefore extra large intestines（Urechis uniconctus）And a kind of research marine organisms live
The good material of property material.Extra large intestines also known as " extra large chicken ", are produced in the fisherman of northern Shandong in Chinese only Bohai Sea Gulf, softly compacted
Dynamic, impulse- free robustness, light yellow from head to foot.It is called " nude sea cucumber " in some local people, extra large intestines not only look like nude sea cucumber,
Its nutritive value is also in no way inferior compared with sea cucumber.But for many years, people use it as " bait ".Really it is used for making
The history of cook dish only decades.Population can be eaten, and had the function that warm filling liver kidney, establishing-Yang controlling nocturnal emission with astringent drugs, be particularly suitable for
Male eats.
Active peptide is the general name of more than 1,000 kinds of peptide.It grows people's, metabolism, disease and aging, dead
During play key effect.Active peptide is most important active material in human body.Exactly because its secretory volume in vivo
Increase or reduce, the mankind is had childhood, in childhood, adult is old until the cycle of death.And take active peptide and just break
This cycle of life, so as to reach extending life, the miraculous effects of effective delaying senility.Peptide in vivo as carrier and
Means of transport, by the nutrient delivery that people are eaten usually to each position of human body, give full play to its function；Peptide is made in vivo
For neurotransmitter, transmission information；Peptide has extremely strong activity and diversity, fully adjustable human body physiological function, strengthens human body
Physiologically active；Peptide can not only provide the nutriment needed for growth in humans's development, and have special biological function, can prevent
Control thrombus, high fat of blood, hypertension, anti-aging is antifatigue, improve immunity of organisms, promote human body to protein, vitamin,
A variety of absorptions to human body healthy trace elements with household such as amino acid, calcium, iron, zinc, selenium, magnesium, copper；Some small peptides have former food protein
Matter or other composition unexistent important physiological functions of amino acid.The less small peptide of some molecular weight in biologically active peptide, with
Amino acid transport function phase ratio, the small peptide in biologically active peptide, which has, absorbs quick, low consumption and undersaturated feature.
Oyster functional is no doubt a lot, but because molecular weight of albumen is bigger, so general eating method is digested and assimilated and compared
It is few.Existing deep processing is mostly oyster shell product, also serves as traditional Chinese medicine in clinical practice.And oyster meat is in addition to eating raw, just
Oyster jerky is machined to, or processing refining oyster sauce is sold, and extra large intestines are also merely possible to dish raw material and sold in seafood market,
This can not more give full play to oyster, the health-care effect of extra large intestines there is the wasting of resources simultaneously.Therefore how will there are raw hundred arteries and veins
Blood, tonifying kidney and benefiting sperm, the multiple functions such as impotence is treated in establishing-Yang, disease of dispelling except labor, nourishing yin to promote diuresis, correction softening hard masses, logical intestines are moisturized and have complement
Gas, beneficial the five internal organs, deficient, beautifying face and moistering lotion of dispelling, kidney-reinforcing Yang-strengthening, antifatigue, antitumor, antiviral, enhancing immunity of organisms and rush are thin
The oyster of pharmacological activity, extra large intestines and the Chinese herbal medicines such as intracellular growth effect make function and absorb more preferable active peptide, preferably reach
The purpose preventiveed treatment of disease to traditional Chinese medicine, really realizes the modernization of Chinese medicine, it has also become traditional Chinese medicine and technical field of biology are urgently to be resolved hurrily
Problem.
The content of the invention
In order to overcome wastage of material in oyster active material extraction process in the prior art and absorption of human body utilization rate low
Deficiency, the invention provides a kind of preparation method of oyster active peptides, be rich in biologically active peptide in the oyster active peptides, have
Tonifying primordial Qi, beneficial the five internal organs, deficient, beautifying face and moistering lotion of dispelling, kidney-reinforcing Yang-strengthening, it is antifatigue, antitumor, antiviral, enhancing immunity of organisms and
The pharmacological activity such as promoting growth of cell effect, and be easy to digest and assimilate, nutritious body utilization rate is high.A seed oyster of the invention
The preparation method of active peptide is using oyster, extra large intestines and Chinese herbal medicine as primary raw material, takes Traditional Chinese Medicine formula and rigorous Chinese medicine big gun
Specification processed, it is prepared with the advanced processing technology of modern biotechnology, its process scientific and precise, strong operability.
The purpose of the present invention is achieved through the following technical solutions：A kind of preparation method of oyster active peptides, its is special
Part is to include oyster active peptides, extra large intestines active peptide, active Chinese drug component peptide, oligoisomaltose composition.Its component is respectively：It is male
Oyster active peptide 50%-80%；Extra large intestines active peptide 5%-20%；Active Chinese drug component peptide 1%-5%；Oligoisomaltose 5%-15%；The component
Percentage by weight sum is 100%.
Oyster active peptides of the present invention are using fresh oyster meat as raw material, and cleaned, homogenate, enzymolysis, centrifugation form
Oyster active peptides liquid.
Extra large intestines active peptide of the present invention is using new fresh ocean gut as raw material, and cleaned, homogenate, enzymolysis, centrifugation form
Extra large intestines active peptide liquid.
Active Chinese drug component peptide of the present invention is with hawthorn, arillus longan, the root of kudzu vine, ginseng, rhodiola root, the fruit of Chinese wolfberry, cultivated land, Huang
Combination raw materials more than one or both of stilbene, Momordica grosvenori, donkey-hide gelatin, flower glue, after being processed using traditional Chinese medicine concocted specification, warp
The Chinese herbal medicine active peptide liquid that broken, boiling, enzymolysis, centrifugation form.
Oligoisomaltose of the present invention is excipient or sweetener, and feature prebiotics.
A kind of preparation method of oyster active peptides of the present invention, it is characterised in that implement through the following steps：
1st, fresh and alive oyster meat is cleaned up and oyster homogenate is made with historrhexis's crusher machine；PH is adjusted into the oyster meat homogenate of preparation
Value 6-8, the 0.1-1% of material gross weight proteolytic enzyme is added, digested under temperature conditionss between 45-60 degrees Celsius of temperature
After 4-8 hours, the dusty yeast for adding material gross weight 0.5%-1% is stirred, and temperature control is taken off between 30-50 Celsius
Raw meat processing, temperature is adjusted to more than 85 degree water-baths 5-15 minutes progress enzyme deactivation Celsius after 1-2 hours, centrifuged after cooling, centrifuge
Rotating speed 8000-13000rpm or so, oyster active peptides liquid is produced, it is standby to place refrigeration；
2nd, fresh and alive extra large intestines are cleaned up and extra large intestines homogenate is made with historrhexis's crusher machine；PH value 6- is adjusted into the extra large intestines homogenate of preparation
8, the 0.1-1% of material gross weight proteolytic enzyme is added, it is small that 4-8 is digested under temperature conditionss between 45-60 degrees Celsius of temperature
Shi Hou, the dusty yeast for adding material gross weight 0.5%-1% are stirred, and temperature control is carried out at deodorant between 30-50 Celsius
Reason, temperature is adjusted to more than 85 degree water-baths 5-15 minutes progress enzyme deactivation Celsius after 1-2 hours, centrifuged after cooling, centrifuge speed
8000-13000rpm or so, extra large intestines active peptide liquid is produced, it is standby to place refrigeration；
3rd, the preparation of active Chinese drug component peptide：Chinese herbal medicine is weighed, break process after being processed by Chinese medicine preparation specification；Add Chinese herbal medicine 10-
20 times of water cooled down after 60-90 minutes are boiled in heating, crosses 60 mesh sieves and filters off the dregs of a decoction, adds cellulase, pectase, pawpaw egg
White enzyme（1:1:1）Multienzyme enzymolysis is carried out, enzyme dosage is that every gram of Chinese medicine adds 3000-8000 enzyme-activity unit（u）, temperature control exists
Between 45-60 degrees Celsius, 2-4 hours are digested；Enzymolysis cools down centrifugation, centrifugal basket after finishing boiling water heating bath enzyme deactivation 10-20 minutes
Fast 4000-10000rpm or so, Chinese herbal medicine active peptide liquid is produced, it is standby to place refrigeration；
4th, allocate：The medium-height grass for taking oyster active peptides liquid, step 3 that oyster active peptides liquid, step 2 that step 1 obtains obtains to obtain
Medicine active peptide liquid, weigh oligoisomaltose powder and carry out compound allotment；
5th, deployed oyster hybrid peptide liquid, which puts stainless steel cask and enters steam sterilization cabinet, is sterilized, and steam sterilization cabinet is warming up to 105-
120 degrees Celsius of sterilization 15-40 minutes, pure water can be added filling into oyster activity according to oral liquid process specification after cooling
Peptide oral liquid product；Oyster hybrid peptide liquid can be freeze-dried into bottled according to freeze dryer on the filling lyophilized bottle of lyophilized technique specification
Oyster active peptides freeze-dried powder product；Oyster hybrid peptide liquid can be spray-dried or is freeze-dried and be prepared into powdery granule, by glue
Tibetan household slave's production. art specification is filling into oyster active peptides capsule product；
6th, step 1 of the present invention, the proteolytic enzyme described in step 2 be trypsase, flavor protease, compound protease, pawpaw
Combination more than one or both of protease, neutral proteinase；
7th, step 1, step 2 and step 3 of the present invention, the donkey-hide gelatin that oyster, extra large intestines can be homogenized and crushed in practical operation
Powder, flower rubber powder are mixed and carry out enzymolysis operation simultaneously, so save resource.
The present invention is raw material from oyster, extra large intestines, Chinese herbal medicine, generates a large amount of active peptide components by controlled enzymatic hydrolysis, it is sought
Support value and absorption rate and edible safety greatly improves, various pharmacological activity enhancings, and the raw meat for alleviating Chinese medicine is bitter
Taste, it is aided with the oligoisomaltose with prebiotics title, not only improves mouthfeel, also breed gut flora, improve stomach
Gut function, active ingredient is set preferably to absorb.A kind of oyster active peptides of the present invention are filling lyophilized according to lyophilized technique specification
Freeze dryer was freeze-dried into bottled freeze-dried powder product, the additive-free preservative of vacuum freeze drying, safe, shelf-life on bottle
It is long；Pure water can be added filling into oral liquid product, distinctive natural flavour mountaineous good mouthfeel according to oral liquid process specification；Will
Hybrid peptide liquid is spray-dried or freeze-drying is prepared into powdery granule, filling into capsule product by capsule manufacturing techniques specification,
Take, be easy to carry.Preparation technology of the present invention is rigorous rationally, strong operability, is especially suitable for large-scale production.
Embodiment
With reference to embodiment, the present invention will be further described
Embodiment one, 1, the fresh oyster 50kg of selection, new fresh ocean gut 10kg are cleaned up and homogenate are made with historrhexis's crusher machine；
PH7 is adjusted into homogenate, trypsase, papain, flavor protease is added and carries out multienzyme enzymolysis, enzyme ratio of components is 1：1：1, enzyme
Dosage is that 6000 enzyme-activity units (u) are added in every gram of homogenate, and temperature control adds oyster homogenate after being digested 6 hours at 55 degree Celsius
The dusty yeast of weight 0.8% stirs, and temperature control carries out defishying, boiling water heating bath enzyme deactivation after 1.5 hours at 35 degree Celsius
Cooling carries out melt cinder separation with supercentrifuge after 15 minutes, and centrifuge speed is adjusted to 12000rpm or so, produces oyster mixing
Active peptide liquid；
2nd, the preparation of active Chinese drug component peptide：Weigh in root of kudzu vine 3kg, rhodiola root 2.kg, fruit of Chinese wolfberry 2.kg, hawthorn 2kg, Momordica grosvenori 1kg
Herbal medicine, break process after being processed by Chinese medicine preparation specification；The water of 15 times of Chinese herbal medicine of addition cooled down after heating boils 60 minutes,
Cross 60 mesh sieves and filter off the dregs of a decoction, add cellulase, pectase, papain（1:1:1）Multienzyme enzymolysis is carried out, enzyme dosage is every gram
Chinese medicine adds 3000 enzyme-activity units（u）, temperature control is at 55 degrees Celsius, enzymolysis 2 hours；Enzymolysis finishes boiling water heating bath enzyme deactivation 10
Centrifugation, centrifuge speed 6000rpm or so are cooled down after minute, produces Chinese herbal medicine active peptide liquid；
3rd, allocate：The Chinese herbal medicine active peptide liquid for taking oyster active peptides liquid, step 2 that step 1 obtains to obtain, weighs oligomeric different malt
Icing Sugar 15kg carries out compound allotment；
4th, the deployed oyster active peptides liquid of step 3 is put into stainless steel cask enter steam sterilization cabinet and sterilized, steam sterilization cabinet liter
Temperature is sterilized 40 minutes to 105 degrees Celsius, filling to freeze dryer vacuum freeze drying on cillin bottle by lyophilized technique specification after cooling,
Produce oyster active peptides freeze-dried powder product of the present invention；Add pure water filling by oral liquid process specification oyster active peptides liquid
To oral liquid bottle, the oral liquid product of oyster active peptides of the present invention is produced；By oyster active peptides liquid it is freeze-dried or spray drying
Powdery granule is prepared into, it is canned into capsule by capsule manufacturing techniques specification, produce oyster active peptides capsule product of the present invention.
Embodiment two, 1, the fresh oyster 60kg of selection, new fresh ocean gut 10kg are cleaned up and are made with historrhexis's crusher machine
Oyster is homogenized；Donkey-hide gelatin block 5kg is ground into 60-80 mesh gelatin powders with pulverizer and is mixed into oyster homogenate, PH6.5 is adjusted into homogenate, added
Enter neutral proteinase, papain, flavor protease and carry out multienzyme enzymolysis, enzyme ratio of components is 1：1：1, enzyme dosage is every gram male
Oyster mixing homogenate 8000 enzyme-activity units (u) of addition, temperature control add oyster mixing homogenate after being digested 6 hours at 55 degree Celsius
The dusty yeast of weight 1% stirs, and temperature control carries out defishying, boiling water heating bath enzyme deactivation 15 after 1.5 hours at 35 degree Celsius
Cooling carries out melt cinder separation with supercentrifuge after minute, and centrifuge speed is adjusted to 13000rpm or so, produces oyster mixing and lives
Property peptide liquid；
2nd, the preparation of active Chinese drug component peptide：Root of kudzu vine 3kg, ginseng 1kg, Radix Astragali 2kg fruits of Chinese wolfberry 2kg, Momordica grosvenori 1kg Chinese herbal medicines are weighed,
Break process after being processed by Chinese medicine preparation specification；The water of 12 times of Chinese herbal medicine of addition cooled down after heating boils 90 minutes, crosses 60
Mesh sieve filters off the dregs of a decoction, adds cellulase, pectase, papain（1:1:1）Multienzyme enzymolysis is carried out, enzyme dosage is every gram of Chinese medicine
Add 4000 enzyme-activity units（u）, temperature control is at 55 degrees Celsius, enzymolysis 2.5 hours；Enzymolysis finishes boiling water heating bath enzyme deactivation 10 and divided
Centrifugation, centrifuge speed 5500rpm or so are cooled down after clock, produces Chinese herbal medicine active peptide liquid；
3rd, allocate：The Chinese herbal medicine active peptide liquid for taking oyster mixed active peptide liquid, step 2 that step 1 obtains to obtain, is weighed oligomeric different
Maltitol powder 15kg carries out compound allotment；
4th, the deployed oyster mixed active peptide liquid of step 3 is put into stainless steel cask enter steam sterilization cabinet and sterilized, steam sterilizing
Cabinet is warming up to 115 degrees Celsius and sterilized 35 minutes, by freeze dryer vacuum refrigeration on filling to the lyophilized bottle of lyophilized technique specification after cooling
Dry, produce oyster active peptides freeze-dried powder product of the present invention；Pure water is added to produce work by oral liquid oyster mixed active peptide liquid
Skill specification is filling to oral liquid bottle, produces the oral liquid product of oyster active peptides of the present invention；Oyster mixed active peptide liquid is chilled
Dry or spray drying is prepared into powdery granule, it is canned into capsule by capsule manufacturing techniques specification, produce oyster of the present invention and live
Property peptide capsule product.
Embodiment three, 1, the preparation of oyster active peptides liquid：Choose fresh oyster 53kg, new fresh ocean gut 8kg cleans up use
Oyster mixing homogenate is made in historrhexis's crusher machine；Donkey-hide gelatin block 3kg is ground into 60-80 mesh gelatin powders with pulverizer and is mixed into oyster
Mixing homogenate；Homogenate is mixed and adjusts PH7.5, compound protease, papain, neutral proteinase is added and carries out multienzyme enzymolysis,
Enzyme ratio of components is 1：1：1, enzyme dosage is every gram of oyster mixing homogenate 9000 enzyme-activity units (u) of addition, and temperature control is Celsius
55 degree of enzymolysis add extra large stichopus japonicus mixing homogenate weight 1% dusty yeast after 5 hours stirs, and temperature control is entered at 35 degree Celsius
Row defishying, cooling carries out melt cinder separation, centrifuge speed with supercentrifuge after boiling water heating bath enzyme deactivation 10 minutes after 1.5 hours
Degree is adjusted to 11000rpm or so, produces oyster mixed active peptide liquid；
2nd, the preparation of active Chinese drug component peptide：Weigh root of kudzu vine 3kg, rhodiola root 2kg, Radix Astragali 2kg fruits of Chinese wolfberry 2kg, arillus longan 1kg medium-height grass
Medicine, break process after being processed by Chinese medicine preparation specification；The water of 10 times of Chinese herbal medicine of addition cooled down after heating boils 90 minutes, mistake
60 mesh sieves filter off the dregs of a decoction, add cellulase, pectase, papain（1:1:1）Multienzyme enzymolysis is carried out, enzyme dosage is in every gram
Medicine adds 4500 enzyme-activity units（u）, temperature control is at 55 degrees Celsius, enzymolysis 3 hours；Enzymolysis finishes boiling water heating bath enzyme deactivation 10 and divided
Centrifugation, centrifuge speed 6000rpm or so are cooled down after clock, produces Chinese herbal medicine active peptide liquid；
3rd, allocate：The Chinese herbal medicine active peptide liquid for taking extra large stichopus japonicus mixed active peptide liquid, step 2 that step 1 obtains to obtain, is weighed oligomeric
Isomaltose powder 16kg carries out compound allotment；
4th, the deployed oyster mixed active peptide liquid of step 3 is put into stainless steel cask enter steam sterilization cabinet and sterilized, steam sterilizing
Cabinet is warming up to 110 degrees Celsius and sterilized 35 minutes, by freeze dryer vacuum refrigeration on filling to the lyophilized bottle of lyophilized technique specification after cooling
Dry, produce oyster active peptides freeze-dried powder product of the present invention；Pure water is added to produce work by oral liquid oyster mixed active peptide liquid
Skill specification is filling to oral liquid bottle, produces the oral liquid product of oyster active peptides of the present invention；Oyster mixed active peptide liquid is chilled
Dry or spray drying is prepared into powdery granule, it is canned into capsule by capsule manufacturing techniques specification, produce oyster of the present invention and live
Property peptide capsule product.
Example IV, 1, the preparation of oyster active peptides liquid：Choose fresh oyster 40kg, new fresh ocean gut 5kg cleans up use
Oyster mixing homogenate is made in historrhexis's crusher machine；Donkey-hide gelatin block 2kg, flower glue 3kg are ground into 60-80 mesh powder with pulverizer and be mixed into
Oyster mixing homogenate；Homogenate is mixed and adjusts PH7, compound protease, trypsase, neutral proteinase is added and carries out multienzyme enzymolysis,
Enzyme ratio of components is 1：1：1, enzyme dosage is every gram of oyster mixing homogenate 8500 enzyme-activity units (u) of addition, and temperature control is Celsius
55 degree of enzymolysis add oyster mixing homogenate weight 1% dusty yeast after 5 hours stirs, and temperature control is in 35 degree Celsius progress
Defishying, cooling carries out melt cinder separation, centrifuge speed with supercentrifuge after boiling water heating bath enzyme deactivation 10 minutes after 1.5 hours
10000rpm or so is adjusted to, produces oyster mixed active peptide liquid；
2nd, the preparation of active Chinese drug component peptide：Root of kudzu vine 3kg, rhodiola root 2kg, cultivated land 2kg fruits of Chinese wolfberry 2kg, hawthorn 1kg Chinese herbal medicines are weighed,
Break process after being processed by Chinese medicine preparation specification；The water of 12 times of Chinese herbal medicine of addition cooled down after heating boils 90 minutes, crosses 60
Mesh sieve filters off the dregs of a decoction, adds cellulase, pectase, papain（1:1:1）Multienzyme enzymolysis is carried out, enzyme dosage is every gram of Chinese medicine
Add 4000 enzyme-activity units（u）, temperature control is at 52 degrees Celsius, enzymolysis 3.5 hours；Enzymolysis finishes boiling water heating bath enzyme deactivation 10 and divided
Centrifugation, centrifuge speed 5000rpm or so are cooled down after clock, produces Chinese herbal medicine active peptide liquid；
3rd, allocate：The Chinese herbal medicine active peptide liquid for taking oyster mixed active peptide liquid, step 2 that step 1 obtains to obtain, is weighed oligomeric different
Maltitol powder 12kg carries out compound allotment；
4th, the deployed oyster mixed active peptide liquid of step 3 is put into stainless steel cask enter steam sterilization cabinet and sterilized, steam sterilizing
Cabinet is warming up to 120 degrees Celsius and sterilized 25 minutes, by freeze dryer vacuum refrigeration on filling to the lyophilized bottle of lyophilized technique specification after cooling
Dry, produce oyster active peptides freeze-dried powder product of the present invention；Pure water is added to produce work by oral liquid oyster mixed active peptide liquid
Skill specification is filling to oral liquid bottle, produces the oral liquid product of oyster active peptides of the present invention；Oyster mixed active peptide liquid is chilled
Dry or spray drying is prepared into powdery granule, it is canned into capsule by capsule manufacturing techniques specification, produce oyster of the present invention and live
Property peptide capsule product.
Embodiment five, 1, the preparation of oyster active peptides liquid：Choose fresh oyster 55kg, new fresh ocean gut 6kg cleans up use
Oyster mixing homogenate is made in historrhexis's crusher machine；Will flower glue 5kg with pulverizer be ground into 60-80 mesh powder be mixed into oyster mix it is even
Slurry；Homogenate is mixed and adjusts PH6.5, papain, trypsase, neutral proteinase is added and carries out multienzyme enzymolysis, enzyme ratio of components
For 1：1：1, enzyme dosage is every gram of oyster mixing homogenate 8000 enzyme-activity units (u) of addition, and temperature control digests 4 at 55 degree Celsius
Hour after add oyster mixing homogenate weight 1% dusty yeast stir, temperature control 35 degree Celsius progress defishyings, 1
Cooling carries out melt cinder separation with supercentrifuge after boiling water heating bath enzyme deactivation 10 minutes after hour, and centrifuge speed is adjusted to 8000rpm
Left and right, produce oyster mixed active peptide liquid；
2nd, the preparation of active Chinese drug component peptide：Weigh in root of kudzu vine 2kg, rhodiola root 1.5kg, arillus longan 1.5kg fruits of Chinese wolfberry 2kg, Radix Astragali 1kg
Herbal medicine, break process after being processed by Chinese medicine preparation specification；The water of 12 times of Chinese herbal medicine of addition cooled down after heating boils 70 minutes,
Cross 80 mesh sieves and filter off the dregs of a decoction, add cellulase, pectase, papain（1:1:1）Multienzyme enzymolysis is carried out, enzyme dosage is every gram
Chinese medicine adds 4500 enzyme-activity units（u）, temperature control is at 53 degrees Celsius, enzymolysis 2.5 hours；Enzymolysis finishes boiling water heating bath enzyme deactivation
Centrifugation, centrifuge speed 5000rpm or so are cooled down after 10 minutes, produces Chinese herbal medicine active peptide liquid；
3rd, allocate：The Chinese herbal medicine active peptide liquid for taking oyster mixed active peptide liquid, step 2 that step 1 obtains to obtain, is weighed oligomeric different
Maltitol powder 10kg carries out compound allotment；
4th, the deployed oyster mixed active peptide liquid of step 3 is put into stainless steel cask enter steam sterilization cabinet and sterilized, steam sterilizing
Cabinet is warming up to 120 degrees Celsius and sterilized 15 minutes, by freeze dryer vacuum refrigeration on filling to the lyophilized bottle of lyophilized technique specification after cooling
Dry, produce oyster active peptides freeze-dried powder product of the present invention；Pure water is added to produce work by oral liquid oyster mixed active peptide liquid
Skill specification is filling to oral liquid bottle, produces the oral liquid product of oyster active peptides of the present invention；Oyster mixed active peptide liquid is chilled
Dry or spray drying is prepared into powdery granule, it is canned into capsule by capsule manufacturing techniques specification, produce oyster of the present invention and live
Property peptide capsule product.
Embodiment six, 1, the preparation of oyster mixed active peptide liquid：It is dry to choose fresh oyster 65kg, new fresh ocean gut 12kg cleaning
Only oyster mixing homogenate is made with historrhexis's crusher machine；Flower glue 5kg with pulverizer is ground into 60-80 mesh powder it is mixed into oyster and mixes
Close homogenate；Homogenate is mixed and adjusts pH value 6.5, papain, flavor protease, neutral proteinase is added and carries out multienzyme enzymolysis,
Enzyme ratio of components is 1：1：1, enzyme dosage is every gram of oyster mixing homogenate 10000 enzyme-activity units (u) of addition, and temperature control is Celsius
55 degree of enzymolysis add oyster mixing homogenate weight 1% dusty yeast after 6 hours stirs, and temperature control is in 35 degree Celsius progress
Defishying, cooling carries out melt cinder separation with supercentrifuge after boiling water heating bath enzyme deactivation 10 minutes after 1 hour, and centrifuge speed is adjusted
To 12000rpm or so, produce oyster mixed active peptide liquid；
2nd, the preparation of active Chinese drug component peptide：Root of kudzu vine 4kg, rhodiola root 3kg, arillus longan 2kg fruit of Chinese wolfberry 3kg Chinese herbal medicines are weighed, by Chinese medicine
Break process after concocted specification processes；The water of 15 times of Chinese herbal medicine of addition cooled down after heating boils 90 minutes, crosses the filter of 60 mesh sieves
The dregs of a decoction are removed, add cellulase, pectase, papain（1:1:1）Multienzyme enzymolysis is carried out, enzyme dosage is that every gram of Chinese medicine adds
6000 enzyme-activity units（u）, temperature control is at 55 degrees Celsius, enzymolysis 3 hours；Enzymolysis is cold after finishing boiling water heating bath enzyme deactivation 10 minutes
But centrifuge, centrifuge speed 6000rpm or so, produce Chinese herbal medicine active peptide liquid；
3rd, allocate：The Chinese herbal medicine active peptide liquid for taking oyster mixed active peptide liquid, step 2 that step 1 obtains to obtain, is weighed oligomeric different
Maltitol powder 20kg carries out compound allotment；
4th, the deployed oyster mixed active peptide liquid of step 3 is put into stainless steel cask enter steam sterilization cabinet and sterilized, steam sterilizing
Cabinet is warming up to 110 degrees Celsius and sterilized 35 minutes, by freeze dryer vacuum refrigeration on filling to the lyophilized bottle of lyophilized technique specification after cooling
Dry, produce oyster active peptides freeze-dried powder product of the present invention；Pure water is added to produce work by oral liquid oyster mixed active peptide liquid
Skill specification is filling to oral liquid bottle, produces the oral liquid product of oyster active peptides of the present invention；Oyster mixed active peptide liquid is chilled
Dry or spray drying is prepared into powdery granule, it is canned into capsule by capsule manufacturing techniques specification, produce oyster of the present invention and live
Property peptide capsule product.
Claims (10)
1. a kind of preparation method of oyster active peptides, it is characterized in that lives including oyster active peptides, extra large intestines active peptide, Chinese medicine
Property peptide, oligoisomaltose composition, its component is respectively：Oyster active peptides 50%-80%；Extra large intestines active peptide 5%-20%；Chinese medicine is lived
Property peptide 1%-5%；Oligoisomaltose 5%-15%, the weight percentages of components sum are 100%.
A kind of 2. preparation method of oyster active peptides of the invention according to claim 1, it is characterised in that：Described oyster lives
Property peptide be cleaned, homogenate, enzymolysis, the oyster active peptides liquid that forms of centrifugation using fresh oyster meat as raw material.
A kind of 3. preparation method of oyster active peptides of the invention according to claim 1, it is characterised in that：Described extra large intestines are lived
Property peptide be cleaned, homogenate, enzymolysis, the extra large intestines active peptide liquid that forms of centrifugation using new fresh ocean gut as raw material.
A kind of 4. oyster active peptides of the invention and preparation method thereof according to claim 1, it is characterised in that：Described Chinese medicine
Active peptide be with hawthorn, arillus longan, the root of kudzu vine, ginseng, rhodiola root, the fruit of Chinese wolfberry, Momordica grosvenori, the Radix Astragali, donkey-hide gelatin, flower glue in one kind or
Two or more combination raw materials, after being processed using traditional Chinese medicine concocted specification, the medium-height grass that is formed through broken, boiling, enzymolysis, centrifugation
Medicine active peptide liquid.
A kind of 5. oyster active peptides of the invention and preparation method thereof according to claim 1, it is characterised in that：Described is oligomeric
Isomaltose is excipient or sweetener, and feature prebiotics.
6. a kind of oyster active peptides of the present invention and preparation method thereof according to claim 1-5, it is characterised in that through following step
It is rapid to implement：
（1）, by fresh and alive oyster meat clean up with historrhexis's crusher machine be made oyster be homogenized；The oyster meat of preparation is homogenized and adjusted
PH value 6-8, the 0.1-1% of material gross weight proteolytic enzyme is added, the enzyme under temperature conditionss between 45-60 degrees Celsius of temperature
After solving 4-7 hours, the dusty yeast for adding material gross weight 0.5%-1% is stirred, and temperature control is carried out between 30-50 Celsius
Defishying, temperature is adjusted to more than 85 degree water-baths 5-15 minutes progress enzyme deactivation Celsius after 1-2 hours, centrifuges, centrifuges after cooling
Machine rotating speed 8000-13000rpm or so, produces oyster active peptides liquid；
（2）, fresh and alive extra large intestines are cleaned up extra large intestines be made with historrhexis's crusher machine be homogenized；PH value is adjusted into the extra large intestines homogenate of preparation
6-8, the 0.1-1% of material gross weight proteolytic enzyme is added, 4-7 is digested under temperature conditionss between 45-60 degrees Celsius of temperature
After hour, the dusty yeast for adding material gross weight 0.5%-1% stirs, and temperature control carries out deodorant between 30-50 Celsius
Processing, temperature is adjusted to more than 85 degree water-baths 5-15 minutes progress enzyme deactivation Celsius after 1-2 hours, centrifuged after cooling, centrifugal basket
Fast 8000-13000rpm or so, produce extra large intestines active peptide liquid；
（3）, active Chinese drug component peptide preparation：Chinese herbal medicine is weighed, break process after being processed by Chinese medicine preparation specification；Add Chinese herbal medicine
10-20 times of water cooled down after 60-90 minutes are boiled in heating, crosses 60 mesh sieves and filters off the dregs of a decoction, adds cellulase, pectase, wood
Melon protease（1:1:1）Multienzyme enzymolysis is carried out, enzyme dosage is that every gram of Chinese medicine adds 3000-8000 enzyme-activity unit（u）, temperature control
System digests 2-4 hours between 45-60 degrees Celsius；Enzymolysis cools down centrifugation, centrifugation after finishing boiling water heating bath enzyme deactivation 10-20 minutes
Machine rotating speed 4000-10000rpm or so, produce Chinese herbal medicine active peptide liquid；
（4）, allotment：Take extra large intestines active peptide liquid, step (3) that oyster active peptides liquid, step (2) that step (1) obtains obtains
To active Chinese drug component peptide liquid, weigh oligoisomaltose powder and carry out compound allotment；
（5）, deployed oyster hybrid peptide liquid put stainless steel cask and enter steam sterilization cabinet and sterilized, steam sterilization cabinet is warming up to
105-120 degrees Celsius of sterilization 15-40 minute, pure water can be added filling into oyster according to oral liquid process specification after cooling
Oral liquid of active peptide product；Can by oyster hybrid peptide liquid according on the filling lyophilized bottle of lyophilized technique specification freeze dryer freeze-drying into
Bottled oyster active peptides freeze-dried powder product；Oyster hybrid peptide liquid can be spray-dried or is freeze-dried and be prepared into powdery granule,
It is filling into oyster active peptides capsule product by capsule manufacturing techniques specification.
7. according to claim 6, a kind of preparation method of described oyster active peptides, it is characterised in that：Described enzymolysis oyster
Proteolytic enzyme for trypsase, flavor protease, compound protease, papain, one kind in neutral proteinase or
Two or more combinations.
8. according to claim 6, a kind of preparation method of described oyster active peptides, it is characterised in that：Described enzymolysis sea intestines
Proteolytic enzyme for trypsase, flavor protease, compound protease, papain, one kind in neutral proteinase or
Two or more combinations.
9. according to claim 6, a kind of preparation method of described oyster active peptides, it is characterised in that：Described enzymolysis medium-height grass
Three kinds of enzymes of cellulase, pectase, papain of medicine, it is 1 that it, which forms weight ratio,：1：1.
A kind of 10. preparation method of oyster active peptides according to claim 6, it is characterised in that：Described step (1),
Step (2) and step（3）In donkey-hide gelatin, flower glue oyster can be homogenized in production operation be embodied, extra large intestines be homogenized and powder
Broken gelatin powder, flower rubber powder are mixed and carry out enzymolysis operation simultaneously, so save resource.
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| CN110074360A (en) * | 2019-05-27 | 2019-08-02 | 鲁东大学 | The processing method of one seed oyster ready-to-eat food |
| CN110537698A (en) * | 2018-05-28 | 2019-12-06 | 许昌神飞航天生物科技有限公司 | nutritional preparation suitable for improving bone density of astronaut |
| CN110574884A (en) * | 2019-10-25 | 2019-12-17 | 烟台梵太食品有限公司 | Oyster peptide oral liquid and preparation method thereof |
| CN111513174A (en) * | 2020-04-21 | 2020-08-11 | 武汉新华扬生物股份有限公司 | Compound enzyme preparation for preparing oyster peptide and application thereof |
| CN111642736A (en) * | 2020-06-16 | 2020-09-11 | 杨燕青 | Formula with anti-fatigue, anti-senilism and anticoagulant effects and preparation method thereof |
| CN113088548A (en) * | 2021-04-08 | 2021-07-09 | 东莞市泡一泡生物科技有限公司 | Preparation method of oyster antioxidant active peptide |
| CN113151386A (en) * | 2021-04-16 | 2021-07-23 | 安徽国肽生物科技有限公司 | Oyster peptide with DPP-IV (dipeptidyl peptidase-IV) inhibition function and preparation method and application thereof |
| CN113826907A (en) * | 2020-06-24 | 2021-12-24 | 青岛大螠海创生物科技有限公司 | A method for preparing solid beverage containing Stichopus japonicus peptide, intestinum Stichopi Japonici peptide and Concha Ostreae peptide |
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| CN113151386A (en) * | 2021-04-16 | 2021-07-23 | 安徽国肽生物科技有限公司 | Oyster peptide with DPP-IV (dipeptidyl peptidase-IV) inhibition function and preparation method and application thereof |
| CN113151386B (en) * | 2021-04-16 | 2022-08-16 | 安徽国肽生物科技有限公司 | Oyster peptide with DPP-IV (dipeptidyl peptidase-IV) inhibition function and preparation method and application thereof |
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Application publication date: 20180306 |