CN107737286A - A kind of Chinese veterinary medicinal composition for preventing and treating swine enzootic pneumonia and its preparation method and application - Google Patents
A kind of Chinese veterinary medicinal composition for preventing and treating swine enzootic pneumonia and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to a kind of Chinese veterinary medicinal composition for preventing and treating swine enzootic pneumonia, include the bulk drugs of following parts by weight：5~30 parts of mulberry leaf, 5~20 parts of chrysanthemum, 1~10 part of the tuber of stemona, cordate houttuynia 10 30,3~15 parts of radix glycyrrhizae.In addition, the invention also provides the preparation method and application of above-mentioned Chinese veterinary medicinal composition.The present invention overcomes the defects of Chinese medicine and Western medicine of the prior art, there is clearing heat and eliminating phlegm, relieving cough and asthma to the pig with swine enzootic pneumonia, the effect of strengthening vital QI to eliminate pathogenic factors, cure rate is high, has no toxic side effect, clinical efficacy is notable, drug residue free, drug resistance is not produced, it is uneasy to recur, and the immunity of organisms of ill pig can be improved；Liquid preparation, compared with common pulvis, facilitate pig farm to be administered by drinking water pipe, improve utilization ratio of drug, reduce administration quantity, improve pig farm culture benefit.
Description
Technical field
The invention belongs to field of traditional Chinese veterinary, is related to a kind of Chinese veterinary medicinal composition, in particular it relates to a kind of preventing and treating swine enzootic pneumonia
Chinese veterinary medicinal composition and its preparation method and application.
Background technology
Swine enzootic pneumonia (Mycoplasmal Pneumonia of Swine) is also known as endemic conditions pneumonia (Swine
Enzootic pneumomia), swine mycoplasma pneumonia (Myeoplasmalpneumoriaofswine), porcine mycoplasmal pneumonia.It is
Reported first by Mare, Switzer and isolated mycoplasma from the lung tissue to suffer from an inflammation of the lungs.This disease is a kind of chronic respiratory of pig
Road transmission disease.Cardinal symptom is cough and asthma, and lesion characteristic is confluent bronchopneumonia, often betides veutro before lungs.
This disease is widely present in all over the world, and the incidence of disease is typically 50%~80%.Part province, city pig farm check at home, positive rate
30~50% [.Affected pig long term growth depauperation, raw example decline 15.9%, and efficiency of feed utilization reduces by 13.8%.Highly dense
The large-scale pig farm of raising is spent, once breaking out the disease, can be managed to pig farm and bring very big difficulty with disease control, economic loss is tight
Weight.Mycoplasma pneumonia of swine is typically difficult thoroughly to cure, troubling gradually as stubborn disease.
Veterinary industry is generally changed using bacterin preparation and terramycin, kanamycins, Enrofloxacin and tylosin etc. at present
Learn synthetic drug to prevent and treat swine enzootic pneumonia, cause animal products chemicals residue serious, and easily produce resistance to
The property of medicine, the quality of animal products is had a strong impact on, huge threat is formd to the health of consumer.Therefore, efficient, ring is developed
The medicine that can prevent and control viral disease protect, having no toxic side effect is the task of top priority of current herding worker.
Chinese medicine is that the Chinese nation accumulates the treasure to precipitate in long-term production, life.Treatment by Chinese herbs pig gas
Asthma disease not only has the function that Western medicine, and nontoxic with clearing heat and eliminating phlegm, relieving cough and asthma, the evil effect in righting osmanthus, cure rate height
Side effect, clinical efficacy is more significant, drug residue free, does not produce drug resistance, uneasy to recur, and can improve the body of ill pig
Immunity, it is cheap.With treatment by Chinese herbs swine enzootic pneumonia, many forefathers carried out research.
《Chinese veterinary pharmacopoeia》Maxingshigan in (version second page 716 in 2015) dissipates, and expels the heat-evil, is relievingd asthma only with facilitaing lung
Cough；The effect of with preventing and treating mycoplasmosis, consisting of：Chinese ephedra 30g, semen armeniacae amarae 30g, gypsum 150g, radix glycyrrhizae 30g, crushing are
Into.Wei Wangming (Chinese veterinarian's medical magazine, 2010 (6)：2) combination therapy of Chinese and western swine enzootic pneumonia is used, but letter is merely given as in text
Single treatment by Chinese herbs prescription, and how sick pig Cure, result do not provide clearly.Wang Hongyan etc. (Zhejiang Agriculture science,
2010(1)：178) treatment by Chinese herbs Jinhua swine enzootic pneumonia result of study shows that Chinese medicine therapeutic effect is pretty good, typically only needs 8d
With regard to that can cure.
Chinese patent (publication number：CN103071094B) " it is a kind of prevent and treat swine enzootic pneumonia Chinese medicine composition and its prepare and
Using ", with aster, the tuber of stemona, semen armeniacae amarae, the root bark of white mulberry, batch 6 kinds of Chinese medicines such as leaf, cordate houttuynia are combined by a certain percentage, low,
The medicine of middle and high dosage, the protective rate to swine enzootic pneumonia are respectively 70%, 80%, 90%.Chinese patent (publication number：
CN103947887B) " a kind of feed, Chinese medicine composition and preparation method for being used to prevent and treat swine enzootic pneumonia ", by manyleaf meadowure rhizome and root, punching
Lotus, cordate houttuynia, cymose buckwheat rhizome, wintercherry fruit or calyx, agalloch eaglewood, perillaseed, Buddha's hand, bulbus fritillariae cirrhosae, Snakegourd Fruit, the wind-weed, bamboo juice, Tabasheer, balloonflower root, sea clam
Shell, bryozoatum, aster, birthwort, loguat leaf, ardisia japonica, the Rhododendron dauricum, Radix Astragali, radix glycyrrhizae, little platanthera herb with root, mountain Pueraria lobota and great Ye hair bells, system
Into dry cream, crush；It is 98% through experiment, protective rate 92%, cure rate 60%, effective percentage.Chinese patent (publication number：
CN101433621B) " a kind of Chinese medicine composition for treating swine enzootic pneumonia and its preparation method and application ", composition is elephant hide
Wooden 10~30 parts by weight, the parts by weight of wild marjoram 5~15, the parts by weight of feltwort 8~24；Cure rate is 95.5%.
Preventing and treating to swine enzootic pneumonia at present is still a weak link, lacks effective preparation and means, and it is fast to develop action
Speed, safely and effectively new drug, are still the task of top priority to adapt to breeding enterprise clinical economics.
The content of the invention
The technical problems to be solved by the invention, just it is to provide a kind of Chinese veterinary medicinal composition for preventing and treating swine enzootic pneumonia, it rises
Effect is rapid, safe and effective, and cure rate is high.
In order to solve the above technical problems, the present invention is achieved by the following technical programs：
A kind of Chinese veterinary medicinal composition for preventing and treating swine enzootic pneumonia, include the bulk drug of following parts by weight：5~30 parts of mulberry leaf, chrysanthemum
Spend 5~20 parts, 1~10 part of the tuber of stemona, cordate houttuynia 10-30,3~15 parts of radix glycyrrhizae.
Preferably, the Chinese veterinary medicinal composition includes the bulk drug of following parts by weight：10~20 parts of mulberry leaf, chrysanthemum 10~15
Part, 3~8 parts of the tuber of stemona, 15~25 parts of cordate houttuynia, 5~10 parts of radix glycyrrhizae.
It is highly preferred that the Chinese veterinary medicinal composition includes the bulk drug of following parts by weight：15 parts of mulberry leaf, 12 parts of chrysanthemum, the tuber of stemona 5
Part, cordate houttuynia 20,8 parts of radix glycyrrhizae.
In addition, the invention also provides the preparation method of above-mentioned Chinese veterinary medicinal composition, it is given by technical scheme once
Realize：
A kind of preparation method for the Chinese veterinary medicinal composition for treating swine enzootic pneumonia, comprises the following steps：
(1) various bulk drugs are weighed in proportion, take chrysanthemum, cordate houttuynia, add 8~10 times of chrysanthemums and cordate houttuynia gross weight
Water, steam distillation 4~8 hours, volatile oil is collected, dissolved with a small amount of ethanol, obtain volatile oil ethanol solution, it is standby；Water vapour
Distillating extracting liquid filters, and filtrate and the dregs of a decoction are standby；
(2) after smashing mulberry leaf, the tuber of stemona and radix glycyrrhizae, the dregs of a decoction in step (1) after extraction volatile oil is added and carry out water extraction, are added
The water of 8~10 times of mulberry leaf, the tuber of stemona and radix glycyrrhizae gross weight, refluxing extraction 2 times, 1~2 hour every time, extract solution filtration, concentration；It is dense
Contracting liquid adds volatile oil ethanol solution；It is well mixed, it is fitted into bag and seals, every bag of 500mL, sterilizing is finished product.
Preferably, in the step (1), chrysanthemum, cordate houttuynia is taken, adds 9 times of chrysanthemums and the water of cordate houttuynia gross weight, water vapour
Distillation 5 hours, volatile oil is collected, dissolved with a small amount of ethanol, obtain volatile oil ethanol solution, it is standby；Steam distillation extract solution is filtered
Cross, filtrate and the dregs of a decoction are standby.
Preferably, in the step (2), after mulberry leaf, the tuber of stemona and radix glycyrrhizae are smashed, extraction volatile oil in step (1) is added
The dregs of a decoction afterwards carry out water extraction, add the water of 9 times of mulberry leaf, the tuber of stemona and radix glycyrrhizae gross weight, refluxing extraction 2 times, 1.5 hours every time, extract
Liquid filters, concentration；Concentrate adds volatile oil ethanol solution；It is well mixed, it is fitted into bag and seals, every bag of 500mL, sterilizing is
Finished product.
In addition, the invention also provides a kind of Chinese veterinary medicinal composition for preventing and treating swine enzootic pneumonia is preparing prevention and treatment pig gas
Breathe heavily the application in medicine.
According to Chinese veterinarian's theory, swine enzootic pneumonia is due to that carcass healthy tendency is not consolidated, lung defends defense function decrease, catches cold it
Heresy, or chill exopathogen it is strongly fragrant and change heat caused by lung qi stop up resistance, a surname drop it is not normal." lung governing qi, department's breathing；Main a surname's drop ".Heresy is violated lung and defended, and defends
Gas is strongly fragrant, and lung loses clear a surname, and the symptom such as occur coughing；If disease and evil is not understood, continue in biography, accumulation of heat in the lung, lung Tianjin of burning form phlegm, phlegm
Hot closing, lung network is injured, impairment of purifying and descending function of the lung, Lung Qi upward reversal, and the symptoms such as expiratory dyspnea, acute asthma, abdominal respiration occurs.
Medicinal material for Chinese veterinary medicinal composition of the present invention is as follows：
Mulberry leaf：For moraceae plants mulberry (Morus alba L.) dried leaf；The property of medicine：It is sweet, bitter, salty, tremble with fear；Return lung, Liver Channel；
Effect：Dispelling wind and heat from the body, clearing away the lungheat and moisturizing, suppressing liver-YANG, clear liver and improve vision.
Chrysanthemum：For feverfew chrysanthemum (Chrysanthemum morifolium Ramat.) dry capitulum；Medicine
Property：It is sweet, bitter, be slightly cold；Return lung, Liver Channel；Effect：Dispelling wind and heat from the body, suppressing liver-YANG, clear liver and improve vision.
The tuber of stemona：For Stemonaceae plant radix stemonae sessilifoliae (Stemona sessilifolia (Miq) Miq.), Radix stemonae japonicae
(Stemona japonica (Bl.) Miq.) or radix stemonae tuberosae (Stemona tuberosa Lour.) dried root；Medicine
Property：Sweet, bitter, tepor；Return lung passes through；Effect：Moistening lung to lower qi cough-relieving, desinsection and delousion.
Cordate houttuynia：Fresh herb or drying for saururaceae plant houttuynia cordata (Houttuynia cordata Thunb.)
Aerial part；The property of medicine：It is pungent, be slightly cold；Return lung passes through；Effect：Clearing heat and detoxicating, effect carbuncle apocenosis, inducing diuresis for treating strangurtia.
Radix glycyrrhizae：For glycyrrhizic legume Glycyrrhiza uralensis Fisch., swollen fruit Radix Glycyrrhiza
Inflate Bat. or glycyrrhiza glabra Glycyrrhiza glabra L. dry root and rhizome；The property of medicine：It is sweet, flat；The thoughts of returning home, lung,
Spleen, stomach；Effect：Invigorate the spleen and benefit qi, be clearing heat and detoxicating, dispelling phlegm and alleviating pain, relieving spasm to stop pain, coordinating the drug actions of a prescription.
The beneficial effects of the invention are as follows：The defects of overcoming Chinese medicine and Western medicine of the prior art, to swine enzootic pneumonia
Pig has clearing heat and eliminating phlegm, relieving cough and asthma, the effect of strengthening vital QI to eliminate pathogenic factors, and cure rate is high, has no toxic side effect, clinical efficacy is notable, no medicine
Thing remains, and does not produce drug resistance, uneasy to recur, and can improve the immunity of organisms of ill pig；Liquid preparation, with common pulvis
Compare, facilitate pig farm to be administered by drinking water pipe, improve utilization ratio of drug, reduce administration quantity, improve pig farm culture benefit.
Embodiment
For the ease of it will be appreciated by those skilled in the art that the present invention is described further below in conjunction with embodiment.
Embodiment 1：
1st, composition：
Mulberry leaf 5kg, chrysanthemum 20kg, tuber of stemona 10kg, cordate houttuynia 30kg, radix glycyrrhizae 15kg.
2nd, preparation method：
(1) chrysanthemum 20kg, cordate houttuynia 30kg are taken, adds the water of 8 times of chrysanthemums and cordate houttuynia weight sum, steam distillation 3 is small
When, volatile oil is collected, is dissolved with a small amount of ethanol, obtains volatile oil ethanol solution, it is standby；Steam distillation extract solution filters, filtrate
And the dregs of a decoction are standby；
(2) mulberry leaf 5kg, tuber of stemona 10kg, radix glycyrrhizae 15kg are taken, after smashing, adds the dregs of a decoction after extraction volatile oil in step (1)
Water extraction is carried out, then adds 8 times of mulberry leaf, the tuber of stemona, the water of radix glycyrrhizae weight sum, refluxing extraction 2 times, 1.5 hours every time, extract solution filter
Cross, concentrate；Concentrate adds volatile oil ethanol solution；It is well mixed, it is fitted into bag and seals, every bag of 500mL, sterilizing, thus obtaining the product.
Implementation column 2：
1st, composition：
Mulberry leaf 30kg, chrysanthemum 5kg, tuber of stemona 1kg, cordate houttuynia 10kg, radix glycyrrhizae 5kg.
2nd, preparation method：
(1) chrysanthemum 5kg, cordate houttuynia 10kg are taken, adds the water of 10 times of chrysanthemums and cordate houttuynia weight sum, steam distillation 2 is small
When, volatile oil is collected, is dissolved with a small amount of ethanol, obtains volatile oil ethanol solution, it is standby；Steam distillation extract solution filters, filtrate
And the dregs of a decoction are standby；
(2) mulberry leaf 30kg, tuber of stemona 1kg, radix glycyrrhizae 5kg are taken, after smashing, adds the dregs of a decoction after extraction volatile oil in step (1)
Water extraction is carried out, adds 10 times of mulberry leaf, the tuber of stemona, the water of radix glycyrrhizae weight sum, refluxing extraction 2 times, 1.5 hours every time, extract solution filtered,
Concentration；Concentrate adds volatile oil ethanol solution；It is well mixed, it is fitted into bag and seals, every bag of 500mL, sterilizing, thus obtaining the product.
Implementation column 3：
1st, composition：
Mulberry leaf 10kg, chrysanthemum 15kg, tuber of stemona 8kg, cordate houttuynia 25kg, radix glycyrrhizae 10kg.
2nd, preparation method：
(1) chrysanthemum 15kg, cordate houttuynia 25kg are taken, adds the water of 9 times of chrysanthemums and cordate houttuynia weight sum, steam distillation 4 is small
When, volatile oil is collected, is dissolved with a small amount of ethanol, obtains volatile oil ethanol solution, it is standby；Steam distillation extract solution filters, filtrate
And the dregs of a decoction are standby；
(2) mulberry leaf 10kg, tuber of stemona 8kg, radix glycyrrhizae 10kg are taken, after smashing, adds the dregs of a decoction after extraction volatile oil in step (1)
Water extraction is carried out, adds 9 times of mulberry leaf, the tuber of stemona, the water of radix glycyrrhizae weight sum, refluxing extraction 2 times, 1.5 hours every time, extract solution filtered,
Concentration；Concentrate adds volatile oil ethanol solution；It is well mixed, it is fitted into bag and seals, every bag of 500mL, sterilizing, thus obtaining the product.
Implementation column 4：
1st, composition：
Mulberry leaf 20kg, chrysanthemum 10kg, tuber of stemona 3kg, cordate houttuynia 15kg, radix glycyrrhizae 3kg.
2nd, preparation method：
(1) chrysanthemum 10kg, cordate houttuynia 15kg are taken, adds the water of 8.5 times of chrysanthemums and cordate houttuynia weight sum, steam distillation 4
Hour, volatile oil is collected, is dissolved with a small amount of ethanol, obtains volatile oil ethanol solution, it is standby；Steam distillation extract solution filters, filter
Liquid and the dregs of a decoction are standby；
(2) mulberry leaf 20kg, tuber of stemona 3kg, radix glycyrrhizae 3kg are taken, after smashing, adds the dregs of a decoction after extraction volatile oil in step (1)
Water extraction is carried out, adds 8.5 times of mulberry leaf, the tuber of stemona, the water of radix glycyrrhizae weight sum, refluxing extraction 2 times, 1.5 hours every time, extract solution was filtered
Cross, concentrate；Concentrate adds volatile oil ethanol solution；It is well mixed, it is fitted into bag and seals, every bag of 500mL, sterilizing, thus obtaining the product.
Implementation column 5：
1st, composition：
Mulberry leaf 15kg, chrysanthemum 12kg, tuber of stemona 5kg, cordate houttuynia 20kg, radix glycyrrhizae 8kg.
2nd, preparation method：
(1) chrysanthemum 12kg, cordate houttuynia 20kg are taken, adds the water of 9.5 times of chrysanthemums and cordate houttuynia weight sum, steam distillation 3
Hour, volatile oil is collected, is dissolved with a small amount of ethanol, obtains volatile oil ethanol solution, it is standby；Steam distillation extract solution filters, filter
Liquid and the dregs of a decoction are standby；
(2) mulberry leaf 15kg, tuber of stemona 5kg, radix glycyrrhizae 8kg are taken, after smashing, adds the dregs of a decoction after extraction volatile oil in step (1)
Water extraction is carried out, adds 9.5 times of mulberry leaf, the tuber of stemona, the water of radix glycyrrhizae weight sum, refluxing extraction 2 times, 1.5 hours every time, extract solution was filtered
Cross, concentrate；Concentrate adds volatile oil ethanol solution；It is well mixed, it is fitted into bag and seals, every bag of 500mL, sterilizing, thus obtaining the product.
Implementation column 6：Acute toxicity test in mice
1. purpose
Observe acute toxic reaction of the Chinese veterinary medicinal composition oral administration to mouse of the preparation of the embodiment of the present invention 1, evaluation
The security of Chinese veterinary medicinal composition prepared by the embodiment of the present invention 1.
2. method
Using maximum dosage-feeding test method.Mouse 40, body weight male and female half and half, are randomly divided into by body weight in 25~30g
Control group and by the preparation method medicine group of the embodiment of the present invention 1, every group 20.The medicine group of the embodiment of the present invention 1 is with Cmax
(4.15g crude drugs/1mL concentrates), maximum capacity (0.4mL/10g body weight), gavage 2 times in 24h, 2 minor ticks 6 hours, observation
Animal behavior activity, the state of mind, the fur colour of skin, two just and secretion and changes of weight, continuous 14 days.
3. result
Behavioral activity, the state of mind, the fur colour of skin, two are just after animal administration and the sign such as secretion is showed no abnormal change
Change；Changes of weight (P ＜ 0.05) in normal range (NR)；Observation terminates each main organs no abnormality seen pathology of rear animal cut open inspection and become
Change.
4. conclusion
The medicine of the embodiment of the present invention 1 has no obvious acute toxic reaction, and clinical practice is safe and reliable；Maximum dosage-feeding is
450.00g crude drugs/part mouse weight.
Embodiment 7：Acute toxicity test in mice
1. purpose
Observe acute toxic reaction of the Chinese veterinary medicinal composition oral administration to mouse of the preparation of the embodiment of the present invention 2, evaluation
The security of Chinese veterinary medicinal composition prepared by the embodiment of the present invention 2.
2. method
Using maximum dosage-feeding test method.Mouse 40, body weight male and female half and half, are randomly divided into by body weight in 25~30g
Control group and by the preparation method medicine group of the embodiment of the present invention 2, every group 20.The medicine group of the embodiment of the present invention 2 is with Cmax
(4.30g crude drugs/1mL concentrates), maximum capacity (0.4mL/10g body weight), gavage 2 times in 24h, 2 minor ticks 6 hours, observation
Animal behavior activity, the state of mind, the fur colour of skin, two just and secretion and changes of weight, continuous 14 days.
3. result
Behavioral activity, the state of mind, the fur colour of skin, two are just after animal administration and the sign such as secretion is showed no abnormal change
Change；Changes of weight (P ＜ 0.05) in normal range (NR)；Observation terminates each main organs no abnormality seen pathology of rear animal cut open inspection and become
Change.
4. conclusion
The medicine of the embodiment of the present invention 2 has no obvious acute toxic reaction, and clinical practice is safe and reliable；Maximum dosage-feeding is
450.00g crude drugs/part mouse weight.
Embodiment 8：Acute toxicity test in mice
1. purpose
Observe acute toxic reaction of the Chinese veterinary medicinal composition oral administration to mouse of the preparation of the embodiment of the present invention 3, evaluation
The security of Chinese veterinary medicinal composition prepared by the embodiment of the present invention 3.
2. method
Using maximum dosage-feeding test method.Mouse 40, body weight male and female half and half, are randomly divided into by body weight in 25~30g
Control group and by the preparation method medicine group of the embodiment of the present invention 3, every group 20.The medicine group of the embodiment of the present invention 3 is with Cmax
(4.45g crude drugs/1mL concentrates), maximum capacity (0.4mL/10g body weight), gavage 2 times in 24h, 2 minor ticks 6 hours, observation
Animal behavior activity, the state of mind, the fur colour of skin, two just and secretion and changes of weight, continuous 14 days.
3. result
Behavioral activity, the state of mind, the fur colour of skin, two are just after animal administration and the sign such as secretion is showed no abnormal change
Change；Changes of weight (P ＜ 0.05) in normal range (NR)；Observation terminates each main organs no abnormality seen pathology of rear animal cut open inspection and become
Change.
4. conclusion
The medicine of the embodiment of the present invention 3 has no obvious acute toxic reaction, and clinical practice is safe and reliable；Maximum dosage-feeding is
450.00g crude drugs/part mouse weight.
Embodiment 9：Acute toxicity test in mice
1. purpose
Observe acute toxic reaction of the Chinese veterinary medicinal composition oral administration to mouse of the preparation of the embodiment of the present invention 4, evaluation
The security of Chinese veterinary medicinal composition prepared by the embodiment of the present invention 4.
2. method
Using maximum dosage-feeding test method.Mouse 40, body weight male and female half and half, are randomly divided into by body weight in 25~30g
Control group and by the preparation method medicine group of the embodiment of the present invention 4, every group 20.The medicine group of the embodiment of the present invention 4 is with Cmax
(4.50g crude drugs/1mL concentrates), maximum capacity (0.4mL/10g body weight), gavage 2 times in 24h, 2 minor ticks 6 hours, observation
Animal behavior activity, the state of mind, the fur colour of skin, two just and secretion and changes of weight, continuous 14 days.
3. result
Behavioral activity, the state of mind, the fur colour of skin, two are just after animal administration and the sign such as secretion is showed no abnormal change
Change；Changes of weight (P ＜ 0.05) in normal range (NR)；Observation terminates each main organs no abnormality seen pathology of rear animal cut open inspection and become
Change.
4. conclusion
The medicine of the embodiment of the present invention 4 has no obvious acute toxic reaction, and clinical practice is safe and reliable；Maximum dosage-feeding is
450.00g crude drugs/part mouse weight.
Embodiment 10：Acute toxicity test in mice
1. purpose
Observe acute toxic reaction of the Chinese veterinary medicinal composition oral administration to mouse of the preparation of the embodiment of the present invention 5, evaluation
The security of Chinese veterinary medicinal composition prepared by the embodiment of the present invention 5.
2. method
Using maximum dosage-feeding test method.Mouse 40, body weight male and female half and half, are randomly divided into by body weight in 25~30g
Control group and by the preparation method medicine group of the embodiment of the present invention 5, every group 20.The medicine group of the embodiment of the present invention 5 is with Cmax
(4.75g crude drugs/1mL concentrates), maximum capacity (0.4mL/10g body weight), gavage 2 times in 24h, 2 minor ticks 6 hours, observation
Animal behavior activity, the state of mind, the fur colour of skin, two just and secretion and changes of weight, continuous 14 days.
3. result
Behavioral activity, the state of mind, the fur colour of skin, two are just after animal administration and the sign such as secretion is showed no abnormal change
Change；Changes of weight (P ＜ 0.05) in normal range (NR)；Observation terminates each main organs no abnormality seen pathology of rear animal cut open inspection and become
Change.
4. conclusion
The medicine of the embodiment of the present invention 5 has no obvious acute toxic reaction, and clinical practice is safe and reliable；Maximum dosage-feeding is
450.00g crude drugs/part mouse weight.
The clinical pharrnacokinetics of embodiment 11 are tested
1. purpose
Therapeutic effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 1 is observed, and then evaluates this hair
The validity of bright composition.
2. swine enzootic pneumonia case diagnosis
Tentative diagnosis is made by epidemiology, clinical symptom, pathological change first, is then used and exempted from by laboratory again
Epidemic disease fluorescent technique makees further diagnosis, final to determine swine enzootic pneumonia case.
3. test material
3.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 1；General physicochemical property：Brown liquid；
Concentration：Per 1ml equivalent to crude drug 4.15g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
3.2 experiment pigs
Pig 80 is tested, is swine enzootic pneumonia sick pig, wherein, boar 48, sow 32,40~60kg of body weight, circle
Support, feed is complete diet pellet.
4. test method
4 groups are randomly divided into, every group 25, observation respectively uses the 3 of the Chinese veterinary medicinal composition of the present invention of the preparation of embodiment 1
Individual test group is high dose group, middle dose group, low dose group and the control group treatment dissipated with Maxingshigan.Each group is in body weight, temperature
It is not statistically significant (P ＞ 0.05) compared to difference on degree, symptom, there is comparativity.Chinese veterinary medicinal composition of the present invention, orally
Clothes；Control group medicine Maxingshigan dissipates, and according to dosage 2kg/t complete diet pellets are recommended, stirs, feeds.
The comparison (head) of each group sick pig therapeutic effect of table 1
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 1, high, middle dose group treatment swine enzootic pneumonia
Sick effect, it is good to dissipate control group therapeutic effect compared with Maxingshigan.
5. efficacy assessment standard：
Cure：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit recovers normal；
It is effective：In medication 5d, before the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit is relatively treated
Take an evident turn for the better, but still there are a small amount of clinical symptoms not disappear；
Effectively：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit only has before relatively treating
Part improves or alleviated, but obvious clinical symptoms also be present；
It is invalid：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit does not have any improvement mark
As, or even deteriorating causes sample dead；
Obvious effective rate：(curing number of cases+effective number of cases)/total number of cases；
It is efficient：(curing number of cases+effective number of cases)/total number of cases.
The clinical pharrnacokinetics of embodiment 12 are tested
1. purpose
Therapeutic effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 2 is observed, and then evaluates this hair
The validity of bright composition.
2. swine enzootic pneumonia case diagnosis
Tentative diagnosis is made by epidemiology, clinical symptom, pathological change first, is then used and exempted from by laboratory again
Epidemic disease fluorescent technique makees further diagnosis, final to determine swine enzootic pneumonia case.
3. test material
3.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 2；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.30g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
3.2 experiment pigs
Pig 100 is tested, is swine enzootic pneumonia sick pig, wherein, boar 44, sow 56,40~60kg of body weight, circle
Support, feed is complete diet pellet.
4. test method
4 groups are randomly divided into, every group 25, observation respectively uses the 3 of the Chinese veterinary medicinal composition of the present invention of the preparation of embodiment 2
Individual test group is high dose group, middle dose group, low dose group and the control group treatment dissipated with Maxingshigan.Each group is in body weight, temperature
It is not statistically significant (P ＞ 0.05) compared to difference on degree, symptom, there is comparativity.Chinese veterinary medicinal composition of the present invention, orally
Clothes；Control group medicine Maxingshigan dissipates, and according to dosage is recommended, 2kg/t complete diet pellets, stirs, feeds.
The comparison (head) of each group sick pig therapeutic effect of table 2
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 2, high, middle dose group treatment swine enzootic pneumonia
Sick effect, it is good to dissipate control group therapeutic effect compared with Maxingshigan.
5. efficacy assessment standard：
Cure：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit recovers normal；
It is effective：In medication 5d, before the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit is relatively treated
Take an evident turn for the better, but still there are a small amount of clinical symptoms not disappear；
Effectively：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit only has before relatively treating
Part improves or alleviated, but obvious clinical symptoms also be present；
It is invalid：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit does not have any improvement mark
As, or even deteriorating causes sample dead；
Obvious effective rate：(curing number of cases+effective number of cases)/total number of cases；
It is efficient：(curing number of cases+effective number of cases)/total number of cases.
The clinical pharrnacokinetics of embodiment 13 are tested
1. purpose
Therapeutic effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 3 is observed, and then evaluates this hair
The validity of bright composition.
2. swine enzootic pneumonia case diagnosis
Tentative diagnosis is made by epidemiology, clinical symptom, pathological change first, is then used and exempted from by laboratory again
Epidemic disease fluorescent technique makees further diagnosis, final to determine swine enzootic pneumonia case.
3. test material
3.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 3；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.45g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
3.2 experiment pigs
Pig 80 is tested, is swine enzootic pneumonia sick pig, wherein, boar 48, sow 32,40~60kg of body weight, circle
Support, feed is complete diet pellet.
4. test method
4 groups are randomly divided into, every group 20, observation respectively uses the 3 of the Chinese veterinary medicinal composition of the present invention of the preparation of embodiment 3
Individual test group is high dose group, middle dose group, low dose group and the control group treatment dissipated with Maxingshigan.Each group is in body weight, temperature
It is not statistically significant (P ＞ 0.05) compared to difference on degree, symptom, there is comparativity.Chinese veterinary medicinal composition of the present invention, orally
Clothes；Control group medicine Maxingshigan dissipates, and according to dosage is recommended, 2kg/t complete diet pellets, stirs, feeds.
The comparison (head) of each group sick pig therapeutic effect of table 3
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 3, high, middle dose group treatment swine enzootic pneumonia
Sick effect, it is good to dissipate control group therapeutic effect compared with Maxingshigan.
5. efficacy assessment standard：
Cure：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit recovers normal；
It is effective：In medication 5d, before the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit is relatively treated
Take an evident turn for the better, but still there are a small amount of clinical symptoms not disappear；
Effectively：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit only has before relatively treating
Part improves or alleviated, but obvious clinical symptoms also be present；
It is invalid：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit does not have any improvement mark
As, or even deteriorating causes sample dead；
Obvious effective rate：(curing number of cases+effective number of cases)/total number of cases；
It is efficient：(curing number of cases+effective number of cases)/total number of cases.
The clinical pharrnacokinetics of embodiment 14 are tested
1. purpose
Therapeutic effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 4 is observed, and then evaluates this hair
The validity of bright composition.
2. swine enzootic pneumonia case diagnosis
Tentative diagnosis is made by epidemiology, clinical symptom, pathological change first, is then used and exempted from by laboratory again
Epidemic disease fluorescent technique makees further diagnosis, final to determine swine enzootic pneumonia case.
3. test material
3.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 4；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.50g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
3.2 experiment pigs
Pig 80 is tested, is swine enzootic pneumonia sick pig, wherein, boar 44, sow 36,40~60kg of body weight, circle
Support, feed is complete diet pellet.
4. test method
4 groups are randomly divided into, every group 20, observation respectively uses the 3 of the Chinese veterinary medicinal composition of the present invention of the preparation of embodiment 4
Individual test group is high dose group, middle dose group, low dose group and the control group treatment dissipated with Maxingshigan.Each group is in body weight, temperature
It is not statistically significant (P ＞ 0.05) compared to difference on degree, symptom, there is comparativity.Chinese veterinary medicinal composition of the present invention, orally
Clothes；Control group medicine Maxingshigan dissipates, and according to dosage is recommended, 2kg/t complete diet pellets, stirs, feeds.
The comparison (head) of each group sick pig therapeutic effect of table 4
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 4, high, middle dose group treatment swine enzootic pneumonia
Sick effect, it is good to dissipate control group therapeutic effect compared with Maxingshigan.
5. efficacy assessment standard：
Cure：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit recovers normal；
It is effective：In medication 5d, before the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit is relatively treated
Take an evident turn for the better, but still there are a small amount of clinical symptoms not disappear；
Effectively：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit only has before relatively treating
Part improves or alleviated, but obvious clinical symptoms also be present；
It is invalid：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit does not have any improvement mark
As, or even deteriorating causes sample dead；
Obvious effective rate：(curing number of cases+effective number of cases)/total number of cases；
It is efficient：(curing number of cases+effective number of cases)/total number of cases.
The clinical pharrnacokinetics of embodiment 15 are tested
1. purpose
Therapeutic effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 5 is observed, and then evaluates this hair
The validity of bright composition.
2. swine enzootic pneumonia case diagnosis
Tentative diagnosis is made by epidemiology, clinical symptom, pathological change first, is then used and exempted from by laboratory again
Epidemic disease fluorescent technique makees further diagnosis, final to determine swine enzootic pneumonia case.
3. test material
3.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 5；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.75g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
3.2 experiment pigs
Pig 80 is tested, is swine enzootic pneumonia sick pig, wherein, boar 44, sow 36,40~60kg of body weight, circle
Support, feed is complete diet pellet.
4. test method
4 groups are randomly divided into, every group 20, observation respectively uses the 3 of the Chinese veterinary medicinal composition of the present invention of the preparation of embodiment 5
Individual test group is high dose group, middle dose group, low dose group and the control group treatment dissipated with Maxingshigan.Each group is in body weight, temperature
It is not statistically significant (P ＞ 0.05) compared to difference on degree, symptom, there is comparativity.Chinese veterinary medicinal composition of the present invention, orally
Clothes；Control group medicine Maxingshigan dissipates, and according to dosage is recommended, 2kg/t complete diet pellets, stirs, feeds.
The comparison (head) of each group sick pig therapeutic effect of table 5
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 5, high, middle dose group treatment swine enzootic pneumonia
Sick effect, it is good to dissipate control group therapeutic effect compared with Maxingshigan.
5. efficacy assessment standard：
Cure：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit recovers normal；
It is effective：In medication 5d, before the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit is relatively treated
Take an evident turn for the better, but still there are a small amount of clinical symptoms not disappear；
Effectively：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit only has before relatively treating
Part improves or alleviated, but obvious clinical symptoms also be present；
It is invalid：In medication 5d, the symptom such as sick pig body temperature, cough, asthma, appetite, breathing and spirit does not have any improvement mark
As, or even deteriorating causes sample dead；
Obvious effective rate：(curing number of cases+effective number of cases)/total number of cases；
It is efficient：(curing number of cases+effective number of cases)/total number of cases.
The clinical pharrnacokinetics of embodiment 16 are tested
1. purpose
Preventive effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 1 is observed, and then evaluates this hair
The validity of bright composition.
2. test material
2.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 1；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.15g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
2.2 bacterial strains are extracted and prepared
Cut open inspection, the sterile liver for taking sick pig, spleen, lung, brain or joint fluid inoculation are carried out to the sick pig with swine enzootic pneumonia
After Todd-Hewitt meat soups, 37 DEG C of culture 24h, gram stain microscopy.Microscopy result is Gram-negative bacteria, acellular
Wall.A Ji Mu Shi and Rui Te Albert'stain Albert are in polymorphy, by the bacterium streak inoculation of spherical, ring-type, shaft-like, point-like and the two poles of the earth shape in
Todd-Hewitt nutrient solutions, it is standby on plain agar sheep blood plate.
3.2 experiment pigs
Pig 60 is tested, health, is not suffering from swine enzootic pneumonia, wherein, boar 32, sow 28,40~60kg of body weight,
Stable breeding, feed are complete diet pellet.
4. test method
It is randomly divided into 3 groups, control group 1, control group 2 and test group, every group 20.Wherein, control group 1 and control group
The 2 complete diet pellet feedings dissipated respectively using with the addition of Maxingshigan, Maxingshigan dissipates to be added according to 2kg/t complete diet pellets；Test group is pressed
The product prepared according to 13.46g/kg feeding embodiments 1.After raising 20 days, artificial challenge is carried out to test group and control group 1, used
5 times of dilutions of standby culture, 0.3mL/kg is injected intraperitoneally, observes and records the incidence of disease and the death rate of each group, as a result such as
Table.
The comparison (head) of each group preventive effect of table 6
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 1 there is obvious pre- preventive effect to swine enzootic pneumonia
Fruit.
The death rate：Death toll/total number of cases
Protective rate：(morbidity number+death toll)/total number of cases
The clinical pharrnacokinetics of embodiment 17 are tested
1. purpose
Preventive effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 2 is observed, and then evaluates this hair
The validity of bright composition.
2. test material
2.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 2；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.30g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
2.2 bacterial strains are extracted and prepared
Cut open inspection, the sterile liver for taking sick pig, spleen, lung, brain or joint fluid inoculation are carried out to the sick pig with swine enzootic pneumonia
After Todd-Hewitt meat soups, 37 DEG C of culture 24h, gram stain microscopy.Microscopy result is Gram-negative bacteria, acellular
Wall.A Ji Mu Shi and Rui Te Albert'stain Albert are in polymorphy, by the bacterium streak inoculation of spherical, ring-type, shaft-like, point-like and the two poles of the earth shape in
Todd-Hewitt nutrient solutions, it is standby on plain agar sheep blood plate.
3.2 experiment pigs
Pig 60 is tested, health, is not suffering from swine enzootic pneumonia, wherein, boar 30, sow 30,40~60kg of body weight,
Stable breeding, feed are complete diet pellet.
4. test method
It is randomly divided into 3 groups, control group 1, control group 2 and test group, every group 20.Wherein, control group 1 and control group
The 2 complete diet pellet feedings dissipated respectively using with the addition of Maxingshigan, Maxingshigan dissipates to be added according to 2kg/t complete diet pellets；Test group is pressed
The product prepared according to 12.99g/kg feeding embodiments 2.After raising 20 days, artificial challenge is carried out to test group and control group 1, used
5 times of dilutions of standby culture, 0.3mL/kg is injected intraperitoneally, observes and records the incidence of disease and the death rate of each group, as a result such as
Table.
The comparison (head) of each group preventive effect of table 7
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 2 there is obvious pre- preventive effect to swine enzootic pneumonia
Fruit.
The death rate：Death toll/total number of cases
Protective rate：(morbidity number+death toll)/total number of cases
The clinical pharrnacokinetics of embodiment 18 are tested
1. purpose
Preventive effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 3 is observed, and then evaluates this hair
The validity of bright composition.
2. test material
2.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 3；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.45g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
2.2 bacterial strains are extracted and prepared
Cut open inspection, the sterile liver for taking sick pig, spleen, lung, brain or joint fluid inoculation are carried out to the sick pig with swine enzootic pneumonia
After Todd-Hewitt meat soups, 37 DEG C of culture 24h, gram stain microscopy.Microscopy result is Gram-negative bacteria, acellular
Wall.A Ji Mu Shi and Rui Te Albert'stain Albert are in polymorphy, by the bacterium streak inoculation of spherical, ring-type, shaft-like, point-like and the two poles of the earth shape in
Todd-Hewitt nutrient solutions, it is standby on plain agar sheep blood plate.
3.2 experiment pigs
Pig 60 is tested, health, is not suffering from swine enzootic pneumonia, wherein, boar 32, sow 28,40~60kg of body weight,
Stable breeding, feed are complete diet pellet.
4. test method
It is randomly divided into 3 groups, control group 1, control group 2 and test group, every group 20.Wherein, control group 1 and control group
The 2 complete diet pellet feedings dissipated respectively using with the addition of Maxingshigan, Maxingshigan dissipates to be added according to 2kg/t complete diet pellets；Test group is pressed
The product prepared according to 12.55g/kg feeding embodiments 3.After raising 20 days, artificial challenge is carried out to control group 1 and test group, used
5 times of dilutions of standby culture, 0.3mL/kg is injected intraperitoneally, observes and records the incidence of disease and the death rate of each group, as a result such as
Table.
The comparison (head) of each group preventive effect of table 8
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 3 there is obvious pre- preventive effect to swine enzootic pneumonia
Fruit.
The death rate：Death toll/total number of cases
Protective rate：(morbidity number+death toll)/total number of cases
The clinical pharrnacokinetics of embodiment 19 are tested
1. purpose
Preventive effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 4 is observed, and then evaluates this hair
The validity of bright composition.
2. test material
2.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 4；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.50g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
2.2 bacterial strains are extracted and prepared
Cut open inspection, the sterile liver for taking sick pig, spleen, lung, brain or joint fluid inoculation are carried out to the sick pig with swine enzootic pneumonia
After Todd-Hewitt meat soups, 37 DEG C of culture 24h, gram stain microscopy.Microscopy result is Gram-negative bacteria, acellular
Wall.A Ji Mu Shi and Rui Te Albert'stain Albert are in polymorphy, by the bacterium streak inoculation of spherical, ring-type, shaft-like, point-like and the two poles of the earth shape in
Todd-Hewitt nutrient solutions, it is standby on plain agar sheep blood plate.
3.2 experiment pigs
Pig 60 is tested, health, is not suffering from swine enzootic pneumonia, wherein, boar 32, sow 28,40~60kg of body weight,
Stable breeding, feed are complete diet pellet.
4. test method
It is randomly divided into 3 groups, control group 1, control group 2 and test group, every group 20.Wherein, control group 1 and control group
The 2 complete diet pellet feedings dissipated respectively using with the addition of Maxingshigan, Maxingshigan dissipates to be added according to 2kg/t complete diet pellets；Test group is pressed
The product prepared according to 12.41g/kg feeding embodiments 1.After raising 20 days, artificial challenge is carried out to control group 1 and test group, used
5 times of dilutions of standby culture, 0.3mL/kg is injected intraperitoneally, observes and records the incidence of disease and the death rate of each group, as a result such as
Table.
The comparison (head) of each group preventive effect of table 9
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 4 there is obvious pre- preventive effect to swine enzootic pneumonia
Fruit.
The death rate：Death toll/total number of cases
Protective rate：(morbidity number+death toll)/total number of cases
The clinical pharrnacokinetics of embodiment 20 are tested
1. purpose
Preventive effect of the Chinese veterinary medicinal composition to swine enzootic pneumonia of the preparation of the embodiment of the present invention 5 is observed, and then evaluates this hair
The validity of bright composition.
2. test material
2.1 medicine
Test medicine：The Chinese veterinary medicinal composition of the present invention prepared according to embodiment 5；General physicochemical property：Brown liquid；
Concentration：Per 1mL equivalent to crude drug 4.75g.
Control drug：Maxingshigan dissipates, according to《Chinese veterinary pharmacopoeia》It is prepared by (version second in 2015).
2.2 bacterial strains are extracted and prepared
Cut open inspection, the sterile liver for taking sick pig, spleen, lung, brain or joint fluid inoculation are carried out to the sick pig with swine enzootic pneumonia
After Todd-Hewitt meat soups, 37 DEG C of culture 24h, gram stain microscopy.Microscopy result is Gram-negative bacteria, acellular
Wall.A Ji Mu Shi and Rui Te Albert'stain Albert are in polymorphy, by the bacterium streak inoculation of spherical, ring-type, shaft-like, point-like and the two poles of the earth shape in
Todd-Hewitt nutrient solutions, it is standby on plain agar sheep blood plate.
3.2 experiment pigs
Pig 60 is tested, health, is not suffering from swine enzootic pneumonia, wherein, boar 32, sow 28,40~60kg of body weight,
Stable breeding, feed are complete diet pellet.
4. test method
It is randomly divided into 3 groups, control group 1, control group 2 and test group, every group 20.Wherein, control group 1 and control group
The 2 complete diet pellet feedings dissipated respectively using with the addition of Maxingshigan, Maxingshigan dissipates to be added according to 2kg/t complete diet pellets；Test group is pressed
The product prepared according to 11.76g/kg feeding embodiments 5.After raising 20 days, artificial challenge is carried out to control group 1 and test group, used
5 times of dilutions of standby culture, 0.3mL/kg is injected intraperitoneally, observes and records the incidence of disease and the death rate of each group, as a result such as
Table.
The comparison (head) of each group preventive effect of table 10
As a result show, Chinese veterinary medicinal composition product prepared by the embodiment of the present invention 5 there is obvious pre- preventive effect to swine enzootic pneumonia
Fruit.
The death rate：Death toll/total number of cases
Protective rate：(morbidity number+death toll)/total number of cases
Embodiment described above only expresses some embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.
Claims (7)
1. a kind of Chinese veterinary medicinal composition for preventing and treating swine enzootic pneumonia, it is characterised in that include the bulk drug of following parts by weight：Mulberry leaf 5
~30 parts, 5~20 parts of chrysanthemum, 1~10 part of the tuber of stemona, cordate houttuynia 10-30,3~15 parts of radix glycyrrhizae.
2. Chinese veterinary medicinal composition according to claim 1, it is characterised in that the Chinese veterinary medicinal composition includes following parts by weight
Bulk drug：10~20 parts of mulberry leaf, 10~15 parts of chrysanthemum, 3~8 parts of the tuber of stemona, 15~25 parts of cordate houttuynia, 5~10 parts of radix glycyrrhizae.
3. Chinese veterinary medicinal composition according to claim 2, it is characterised in that the Chinese veterinary medicinal composition includes following parts by weight
Bulk drug：15 parts of mulberry leaf, 12 parts of chrysanthemum, 5 parts of the tuber of stemona, cordate houttuynia 20,8 parts of radix glycyrrhizae.
4. the preparation method of the Chinese veterinary medicinal composition of the treatment swine enzootic pneumonia according to any one of claims 1 to 3, its feature
It is, comprises the following steps：
(1) various bulk drugs are weighed in proportion, take chrysanthemum, cordate houttuynia, add 8~10 times of chrysanthemums and the water of cordate houttuynia gross weight, water
Steam distillation 4~8 hours, volatile oil is collected, dissolved with a small amount of ethanol, obtain volatile oil ethanol solution, it is standby；Steam distillation carries
Liquid is taken to filter, filtrate and the dregs of a decoction are standby；
(2) dregs of a decoction in step (1) after extraction volatile oil will be added and carry out water extraction after mulberry leaf, the tuber of stemona and radix glycyrrhizae smash, add 8~
The water of 10 times of mulberry leaf, the tuber of stemona and radix glycyrrhizae gross weight, refluxing extraction 2 times, 1~2 hour every time, extract solution filtration, concentration；Concentrate
Add volatile oil ethanol solution；It is well mixed, it is fitted into bag and seals, every bag of 500mL, sterilizing is finished product.
5. preparation method according to claim 4, it is characterised in that in the step (1), take chrysanthemum, cordate houttuynia, add 9
The water of times chrysanthemum and cordate houttuynia gross weight, steam distillation 5 hours, volatile oil is collected, dissolved with a small amount of ethanol, obtain volatile oil second
Alcoholic solution, it is standby；Steam distillation extract solution filters, and filtrate and the dregs of a decoction are standby.
6. preparation method according to claim 4, it is characterised in that in the step (2), by mulberry leaf, the tuber of stemona and radix glycyrrhizae
After smashing, add the dregs of a decoction in step (1) after extraction volatile oil and carry out water extraction, add 9 times of mulberry leaf, the tuber of stemona and radix glycyrrhizae gross weight
Water, refluxing extraction 2 times, 1.5 hours every time, extract solution filtration, concentration；Concentrate adds volatile oil ethanol solution；It is well mixed,
It is fitted into bag and seals, every bag of 500mL, sterilizing is finished product.
7. the Chinese veterinary medicinal composition of the treatment swine enzootic pneumonia according to any one of claims 1 to 3 is preparing prevention and treatment
Application in swine enzootic pneumonia medicine.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109832501A (en) * | 2018-07-05 | 2019-06-04 | 四川伟康生物科技有限公司 | Noni fruit compound additive, preparation method and the application method to raise table hens for substituting antibiotic |
| CN113786461A (en) * | 2021-09-29 | 2021-12-14 | 廖勇 | Traditional Chinese medicine formula for treating animal asthma and heat toxin and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130060235A1 (en) * | 2006-12-01 | 2013-03-07 | James Liu | Pharmaceutical compositions and methods of use |
| CN105362868A (en) * | 2015-12-09 | 2016-03-02 | 湖北武当动物药业有限责任公司 | Drug for preventing and treating animal respiratory syndrome |
| CN105396011A (en) * | 2015-12-07 | 2016-03-16 | 黄协珊 | Traditional Chinese medicinal composition for treating cough |
-
2017
- 2017-11-20 CN CN201711158964.9A patent/CN107737286A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130060235A1 (en) * | 2006-12-01 | 2013-03-07 | James Liu | Pharmaceutical compositions and methods of use |
| CN105396011A (en) * | 2015-12-07 | 2016-03-16 | 黄协珊 | Traditional Chinese medicinal composition for treating cough |
| CN105362868A (en) * | 2015-12-09 | 2016-03-02 | 湖北武当动物药业有限责任公司 | Drug for preventing and treating animal respiratory syndrome |
Non-Patent Citations (5)
| Title |
|---|
| 于艳龙等: ""猪气喘病的防治"", 《吉林畜牧兽医》 * |
| 刘福昌等: "《天然药物化学》", 28 February 2013, 华中科技大学出版社 * |
| 梁剑平: "《中兽药学》", 31 January 2014, 军事医学科学出版社 * |
| 荆所义: "《中药防治畜禽疾病实用技术》", 31 August 2011, 中原农民出版社 * |
| 靳明亮: "治疗马气喘症 的常用方药", 《中兽医学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109832501A (en) * | 2018-07-05 | 2019-06-04 | 四川伟康生物科技有限公司 | Noni fruit compound additive, preparation method and the application method to raise table hens for substituting antibiotic |
| CN113786461A (en) * | 2021-09-29 | 2021-12-14 | 廖勇 | Traditional Chinese medicine formula for treating animal asthma and heat toxin and preparation method thereof |
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