CN107709958B - Sampling mechanism, air suction detection method thereof and sample analyzer - Google Patents
Sampling mechanism, air suction detection method thereof and sample analyzer Download PDFInfo
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Abstract
The application discloses sampling mechanism and air suction detection method and sample analyzer thereof, sampling mechanism is including removing subassembly (20), sampling subassembly (10), drive unit (30) and control unit (40), the signal of telecommunication of sign sampling needle physical quantity is gathered respectively around the stock layout to sampling mechanism and/or around the stock suction for judge whether the stock suction reaches the predetermined amount. Because this application still gathers the signal of telecommunication before the stock layout and judges whether the volume of inhaling the sample reaches the predetermined volume, solved the condition that traditional technology cavity inhale the sample and miss reporting.
Description
Technical Field
The invention relates to a sample analysis technology, in particular to a sampling mechanism of a sample analyzer and an air suction detection method thereof.
Background
When a sample analyzer analyzes a sample, a sample with a preset amount is collected from a sample container through a sampling needle, a reagent with a preset amount is collected from a reagent container, the collection of the sample and the reagent is called as sample suction for short, then the collected sample and the reagent are injected into a reaction container for incubation reaction, and finally the reacted sample is detected. When a sample or a reagent is collected, a predetermined amount of the sample or the reagent may not be collected due to some reason (for example, the sample or the reagent runs out), so that aspiration may be caused, wherein aspiration includes that the sample/the reagent is not collected and the collected sample/reagent does not reach the predetermined amount, and finally, the detection result is inaccurate. Therefore, the sample analyzer is generally provided with a suction detection function for detecting whether the amount of the sucked sample reaches a predetermined amount.
The commonly used aspiration detection scheme is: the sampling needle measures the liquid level voltage before sample suction to obtain a voltage value V0After the sample suction is finished, the sampling needle measures the liquid level voltage to obtain a voltage value V1(ii) a Because the sampling needle is in the air before the sample is sucked and the sampling needle is required to be under the residual liquid level after the sample is sucked, the voltages of the two conditions have certain difference, and V is utilized1And V0The difference value can judge whether the sampling needle is below the liquid level after sample suction, and if the sampling needle is still below the liquid level after sample suction, the sampling needle can be known to collect a preset amount of samples or reagents. However, in the process of sample suction, if the sampling needle touches an unexpected object such as the tube wall of the test tube, or air bubbles exist in the sampling needle or the test tube, even if the sampling needle is subjected to vacuum suction, the voltage difference value obtained by the sampling needle before and after sample suction is close to the voltage difference value obtained in the process of smooth sampling by the sampling needle, so that the condition of missed sample suction is caused.
Disclosure of Invention
According to a first aspect, there is provided in an embodiment a sampling mechanism comprising:
a moving assembly;
the sampling assembly comprises a sampling needle and a detection module, the sampling needle is fixed on the moving assembly, the detection module is electrically connected with the sampling needle, and the change of the physical quantity of the sampling needle is detected in real time and converted into an electric signal;
the driving unit is coupled to the moving component and drives the moving component to move so as to drive the sampling needle to move;
the control unit is electrically connected with the driving unit and sends a driving control signal to the driving unit, the control unit is further electrically connected with the detection module and collects an electric signal from the detection module, the control unit judges whether the sample suction amount reaches a preset amount according to a comparison value of a first electric signal and a second electric signal, the first electric signal is an electric signal output by the detection module before the sampling needle executes sample arrangement, and the second electric signal is an electric signal output by the detection module after the sampling needle finishes sample arrangement.
Another embodiment provides another sampling mechanism comprising:
a moving assembly;
the sampling assembly comprises a sampling needle and a detection module, the sampling needle is fixed on the moving assembly, the detection module is electrically connected with the sampling needle, and the change of the physical quantity of the sampling needle is detected in real time and converted into an electric signal;
the driving unit is coupled to the moving component and drives the moving component to move so as to drive the sampling needle to move;
a control unit electrically connected with the drive unit and sending drive control signals to the drive unit, the control unit also electrically connected with the detection module and collecting electric signals from the detection module, the control unit preliminarily judges whether the sample suction amount reaches a predetermined amount based on a comparison value of the first electric signal and the second electric signal, and preliminarily judges whether the sample suction amount reaches the predetermined amount based on a comparison value of the third electric signal and the fourth electric signal, comprehensively judging whether the sample suction amount reaches a preset amount according to the results of the two primary judgments, wherein the first electric signal is an electric signal output by the detection module before the sampling needle performs sample arrangement, the second electrical signal is the electrical signal that detection module output after the sample needle arranges the appearance and finishes, the third electrical signal is the electrical signal that detection module output before the sample needle carries out the appearance of inhaling, the fourth electrical signal is the electrical signal that detection module output after the sample needle finishes inhaling the appearance.
According to a second aspect, there is provided in one embodiment a method of detecting aspiration of a sampling mechanism, the method comprising:
before the sampling needle performs sample arrangement, the detection module detects physical quantity of the sampling needle and outputs a first electric signal;
after the sampling of the sampling needle is finished, the detection module detects the physical quantity of the sampling needle and outputs a second electric signal;
and judging whether the sample suction amount reaches a preset amount or not according to the comparison value of the first electric signal and the second electric signal.
In another embodiment, the method comprises:
before the sampling needle performs sample suction, the detection module detects the physical quantity of the sampling needle and outputs a third electric signal;
after the sampling of the sampling needle is finished, the detection module detects the physical quantity of the sampling needle and outputs a fourth electric signal;
preliminarily judging whether the sample suction amount reaches a preset amount according to a comparison value of the third electric signal and the fourth electric signal;
before the sampling needle performs sample arrangement, the detection module detects physical quantity of the sampling needle and outputs a first electric signal;
after the sampling of the sampling needle is finished, the detection module detects the physical quantity of the sampling needle and outputs a second electric signal;
preliminarily judging whether the sample suction amount reaches a preset amount according to the comparison value of the first electric signal and the second electric signal;
and comprehensively judging whether the sample suction amount reaches a preset amount according to the results of the two primary judgments.
According to a third aspect, there is provided in one embodiment a sample analyzer comprising the sampling mechanism described above.
According to the sampling mechanism, the air suction detection method and the sample analyzer of the sampling mechanism, the detection module collects two electric signals at two moments before and after sample arrangement of the sampling needle, and the control unit judges whether the sample suction amount reaches a preset amount according to the two electric signals, so that the air suction sample judgment is more reliable than that of the traditional method. In addition, in the embodiment, two electric signals can be acquired at two moments before and after the sampling of the sampling needle, the control unit judges whether the sample suction amount reaches the preset amount or not according to the two electric signals, and the control unit finally integrates the two judgments to give a reliable judgment, so that the condition that the empty suction sample is missed to be reported is avoided greatly.
Drawings
Fig. 1 is a schematic structural diagram of a sampling mechanism in an embodiment of the present application;
FIG. 2 is a schematic structural diagram of a portion of a sampling mechanism according to an embodiment of the present disclosure;
FIG. 3 is a flow chart of a hollow suction detection method according to an embodiment of the present application;
FIG. 4 is a schematic view of a sampling mechanism according to an embodiment of the present disclosure;
fig. 5 is a flowchart of a hollow suction detection method according to a second embodiment of the present application.
Detailed Description
When a sample analyzer analyzes a sample, one of the operations is sampling: and collecting predetermined amounts of the sample and the reagent from the sample container and the reagent container respectively by a sampling mechanism. When a predetermined amount of sample or reagent is not collected due to some reason (for example, the sample or reagent runs out), aspiration may be caused, and thus, the detection result of the sample may be inaccurate.
The application provides a sample analyzer, which comprises a sampling mechanism. For traditional sampling mechanism, the sampling mechanism of this application adopts a judgement mechanism to judge whether empty suction when the stock layout to improve the accuracy of testing result. The following is a further description by way of example with reference to the accompanying drawings.
Example one
Referring to fig. 1 and 2, the sampling mechanism of the present disclosure includes a sampling assembly 10, a moving assembly 20, a driving unit 30, and a control unit 40.
The sampling assembly 10 comprises a sampling needle 11 and a detection module 12, the sampling needle 11 is fixed on the moving assembly 20, the detection module 12 is electrically connected with the sampling needle 11, the physical quantity change of the sampling needle 11 is detected in real time and converted into an electric signal, and preferably, the electric signal can be a voltage signal.
There are various implementations of the sampling needle 11, and in this embodiment, the sampling needle 11 includes a double-layer metal needle cylinder nested inside and outside: an inner metal cylinder 111 and an outer metal cylinder 112, wherein the inner metal cylinder 111 is used for sucking, temporarily storing and discharging a sample or a reagent (the sample is not exemplified below), and the outer metal cylinder 112 is fixed on the moving assembly 20. The inner metal cylinder 111 and the outer metal cylinder 112 may be coaxially disposed and insulated from each other, so that the sampling needle 11 may be regarded as a capacitor, and a capacitance value of the capacitor may be changed according to a difference in a sample amount stored in the sampling needle 11 and whether the sampling needle 11 contacts the liquid level, so that a change in a physical amount of the sampling needle 11 may be represented by the capacitance value of the sampling needle 11, and whether the sampling needle contacts the liquid level and collects a sufficient sample amount may be determined by the change. In order to detect the capacitance value of the sampling needle 11, the inner metal cylinder 111 and the outer metal cylinder 112 of the sampling needle 11 are electrically connected to the detection module 12 through a conducting wire.
There are also various implementations of the detection module 12, and in this embodiment, the detection module 12 is fixed on the moving component 20 and includes an oscillator 121, a phase detector 122, a reference signal 123, a filter 124, and an analog-to-digital converter 125. The oscillator 121 is connected with the inner metal cylinder 111 and the outer metal cylinder 112 of the sampling needle 11 by conducting wires, so that the sampling needle 11 is used as a parameter element of a resonant circuit of the oscillator 121, that is, the frequency of a pulse signal output by the oscillator 121 varies with the variation of a physical quantity (e.g., a capacitance value) of the sampling needle 11, and when the capacitance value of the sampling needle 11 varies, the oscillator 121 generates a pulse signal with a corresponding frequency and inputs the pulse signal into the phase detector 122. The phase detector 122 compares the phase of the pulse signal with a reference signal in the reference signal generator 123 and outputs a phase error signal to the filter 124, the filter 124 filters the phase error signal and outputs the phase error signal to the analog-to-digital converter 125, the analog-to-digital converter 125 performs analog-to-digital conversion on the filtered phase error signal and outputs an electrical signal for the control unit 40 to collect, and when the oscillator 121 is a voltage-controlled oscillator, the electrical signal output by the analog-to-digital converter 125 is a voltage signal.
In order to smoothly perform the suction and discharge, the sampling assembly 11 may further include a syringe 13, an output end of the syringe 13 communicates with the inner metal cylinder 111 of the sampling needle 11 through a pipe, and a driving unit 30 is coupled to a push end of the syringe 13 to drive the push end to move. When the pushing end is driven to pull outwards, the sampling needle 11 sucks samples, and when the pushing end is driven to push inwards, the sampling needle 11 discharges samples.
The moving assembly 20 is a three-dimensional or two-dimensional moving mechanism, and can drive the sampling needle 11 fixed thereon to move in a three-dimensional or two-dimensional direction, so that the sampling needle 11 moves to a specified position to complete the sample sucking and discharging operations. There are various implementations of the moving assembly 20, such as a rocker arm structure and a linear slider guide structure.
The driving unit 30 is coupled to the moving component 20, and drives the moving component 20 to move, so as to drive the sampling needle 11 to move.
The control unit 40 is electrically connected to the driving unit 30, and sends a driving control signal to the driving unit 30, and after receiving the driving control signal, the driving unit 30 drives the moving assembly 20 to move according to the driving control signal, so as to move the sampling needle to a specified position, or drives the pushing end of the syringe 13 to move outwards or inwards for a certain distance. The control unit 40 is further electrically connected to the detection module 12, and outputs a circuit parameter to the detection module 12 and collects an electrical signal from the detection module 12, and the control unit 40 determines whether the sample suction amount reaches a predetermined amount according to a comparison value between a first electrical signal and a second electrical signal, wherein the first electrical signal is an electrical signal output by the detection module 12 before the sampling needle 11 performs the sample arrangement, specifically, the first electrical signal may be an electrical signal collected by the control unit 40 from the detection module 12 after the sampling needle 11 moves to the reaction cup position and before the sample arrangement is performed, the second electrical signal is an electrical signal output by the detection module 12 after the sampling needle 11 performs the sample arrangement, specifically, the second electrical signal may be an electrical signal collected by the control unit 40 from the detection module 12 after the sample arrangement position of the sampling needle 11 is completed, the sample arrangement position is a position where the sampling needle 11 performs the sample arrangement operation into the reaction cup, and when the sampling needle performs the sample arrangement operation, should be located at a certain height position in the reaction cup, which is called a stock layout position, the stock layout position is different according to different detection items and specific operations, so that the sampling needle 11 is in the air before stock layout and is below the liquid level of the reaction cup after stock layout. The comparison value of the second electric signal collected in this way with the first electric signal is easier to judge. As described above, the control unit 40 determines whether the sample suction amount reaches the predetermined amount according to the comparison value between the first electrical signal and the second electrical signal, and in this determination mechanism, the control unit 40 may regard that the sample suction amount reaches the predetermined amount when the comparison value is greater than or equal to a set threshold, or else, regard that the sample suction amount does not reach the predetermined amount, that is, the sampling mechanism performs suction. For convenience and rapidness of determination, the comparison value may be a difference or a ratio of the first electrical signal and the second electrical signal.
The above is the sampling mechanism disclosed in this embodiment, and accordingly, this embodiment also discloses a method for detecting suction of the sampling mechanism (hereinafter referred to as suction detection method), which is described in detail below with reference to fig. 3 and 4.
And step S100, sucking the sample. Referring to fig. 4, a sample tube 50 for holding a sample is placed on a sample rack 51, a reaction cup 52 for holding a sample and a reagent is placed on a reaction tray 53, and the sample and the reagent are sequentially injected into the reaction cup 52 for incubation reaction. When a sample sucking operation is performed, the driving unit 30 drives the moving assembly 20 to move according to the driving control signal sent by the control unit 40, so as to drive the sampling needle 11 to move in the horizontal and vertical directions, so that the lower end (the end not connected with the injector 13) of the sampling needle 11 moves to the liquid level in the sample tube 50, and the driving unit 30 drives the pushing end of the injector 13 to pull out for a certain distance according to the driving control signal sent by the control unit 40, so as to generate a suction force, so that the sampling needle 11 sucks a predetermined amount of sample. The driving unit 30 drives the moving assembly 20 to move according to the driving control signal sent by the control unit 40, so that the sampling needle 11 moves upwards above the sample tube 50, and the sample sucking operation is completed.
Step S201: a stock sample is prepared. After the sampling needle 11 finishes the sample suction, the sampling needle is moved from the upper part of the sample tube 50 which executes the sample suction operation to the position of the reaction cup 50 which executes the sample discharge operation, and the sampling needle 11 is controlled to move to the position of the reaction cup and then stops above the reaction cup;
step S202: the control unit 40 collects the present electric signal from the detection module 12 as a first electric signal. In order to stably acquire the first electrical signal, the sampling needle 11 is controlled to stay above the reaction cup 52 for a period of time, for example, 200 microseconds, within which 200 microseconds the control unit 40 detects the current electrical signal from the detection module 12 as the first electrical signal.
Step S203: and controlling the sampling needle 11 to move downwards to a sample arranging position from the upper part of the reaction cup, and executing sample arranging. After the sampling needle 11 moves downwards from the upper part of the reaction cup 52 to the sample arranging position, the driving unit 30 drives the pushing end of the injector 13 to push inwards for a certain distance, so as to generate a pushing force, and the sampling needle 11 injects a preset amount of samples into the reaction cup 52, thereby completing the sample arranging.
Step S204: after the stock removal, the stock removal station is stopped for a predetermined time, for example 200 microseconds, during which the control unit collects the current electrical signal from the detection module 12 as the second electrical signal.
Step S210: and comparing the first electric signal with the second electric signal, and judging whether the sample suction amount reaches a preset amount according to the comparison value of the first electric signal and the second electric signal. In a specific example, the control unit 40 calculates a difference between the first electrical signal and the second electrical signal, and when the difference is greater than or equal to a set threshold, makes a judgment that the sample suction amount reaches a predetermined amount, which indicates that the sample suction and/or the sample discharge is successful in the normal operation, or otherwise, makes a judgment that the sample suction amount does not reach the predetermined amount, which indicates that the suction and/or the sample discharge is failed in the operation. In some embodiments, the control unit 40 may further calculate a ratio of the first electrical signal and the second electrical signal, and determine whether to suck according to the ratio. When it is judged that the amount of the aspirated sample has reached the predetermined amount, the step S301 is continued to execute the next operation (e.g., reagent aspiration or stirring operation), and when it is judged that the amount of the aspirated sample has not reached the predetermined amount and suction is caused, the step S302 is executed to give an alarm and the operation of the sampling needle may be suspended at the same time as the alarm.
The sampling mechanism, the air suction detection method and the sample analyzer collect the first electric signal and the second electric signal respectively before and after the sample is discharged by the sampling needle, so that the problem that the condition that the air suction sample is missed in reporting is easily solved in the prior art, and the problem that whether the sample is normally sucked and discharged is not easily judged when the sample is in a gel state can be solved.
Example two
Based on the first embodiment, the sampling mechanism, the air suction detection method thereof, and the sample analyzer disclosed in this embodiment also collect two electrical signals at two times before and after sample suction for determining whether the sample is normally sucked or air sucked, which will be described in detail below.
On the basis of the first embodiment, the control unit 40 of this embodiment, on one hand, preliminarily determines whether the sample suction amount reaches the predetermined amount according to the comparison value of the first electrical signal and the second electrical signal, on the other hand, preliminarily determines whether the sample suction amount reaches the predetermined amount according to the comparison value of the third electrical signal and the fourth electrical signal, and comprehensively determines whether the sample suction amount reaches the predetermined amount according to the results of the two preliminary determinations, specifically, the control unit 40 considers that the sample suction amount does not reach the predetermined amount (i.e., air suction) when the sample suction amount does not reach the predetermined amount once in the two preliminary determinations, and considers that the sample suction amount reaches the predetermined amount when the sample suction amount reaches the predetermined amount in the two preliminary determinations; the first electrical signal is an electrical signal output by the detection module 12 before the sampling needle 11 performs sample arrangement, the second electrical signal is an electrical signal output by the detection module 12 after the sampling needle 11 performs sample arrangement, the third electrical signal is an electrical signal output by the detection module 12 before the sampling needle 11 performs sample absorption, and the fourth electrical signal is an electrical signal output by the detection module 12 after the sampling needle 11 performs sample absorption.
The specific detection process is shown in fig. 5, and includes the following steps.
Before the sampling needle 11 performs the sample suction, the detection module 12 detects a physical quantity of the sampling needle 11 and outputs a third electrical signal, specifically, it includes steps S101, S102:
step S101: controlling the sampling needle 11 to move to the position of the sample tube and stop above the sample tube;
step S102: the present electrical signal is collected from the detection module 12 as a third electrical signal.
After the sampling by the sampling needle 11 is completed, the detecting module 12 detects the physical quantity of the sampling needle and outputs a fourth electrical signal, specifically, the method includes steps S103 and S104:
step S103: controlling the sampling needle 11 to move downwards from the upper part of the sample tube 50 to a sample sucking position to suck samples, wherein the sample sucking position refers to a position where the sampling needle 11 performs sample sucking operation in the sample tube 50, and the sample sucking position is determined according to the sample amount and the sample sucking amount in the sample tube 50, so that the sampling needle 11 is still positioned under the liquid level after the sample sucking position performs sample sucking;
step S104: stopping for a predetermined time after the sample is drawn, and acquiring the current electric signal from the detection module 12 as a fourth electric signal in the period.
Step S110: and preliminarily judging whether the sample suction amount reaches a preset amount according to the comparison value of the third electric signal and the fourth electric signal. The determination method may be the same as step S210 in the first embodiment. When the sample suction amount reaches the predetermined amount, continuing to execute step S201 to prepare for sample discharge, and continuing to execute steps S202-S210, respectively acquiring the first electrical signal and the second electrical signal from the detection module 12 before and after sample discharge, and determining whether the sample is sucked empty, wherein the specific content of each step is the same as that in the first embodiment, and is not described herein again; when it is judged that the sample suction amount does not reach the predetermined amount and causes suction, step S302 is executed to give an alarm.
In the embodiment, the electric signals are collected before and after sample suction to perform first judgment on whether the sample suction amount reaches the preset amount, the electric signals are collected before and after sample discharge to perform second judgment on whether the sample suction amount reaches the preset amount, and when the first judgment is empty suction, an alarm is given and the next sample discharge operation and the second judgment are stopped. In other embodiments, the alarm may not be performed when the first determination is that suction is empty, but the following sample discharge and second determination are continued, after the second determination is made, the results of the first determination are integrated, an alarm is performed if the result of one determination is that suction is empty, and the next operation is continued only when the results of two determinations are that the suction amount sample reaches the predetermined amount.
In this embodiment, whether the amount of the sample sucked reaches the predetermined amount is determined before and after the sample sucking and before and after the sample discharging, and the final determination is made by combining the results of the two determinations.
The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. Variations of the above-described embodiments may be made by those skilled in the art, consistent with the principles of the invention.
Claims (12)
1. A sampling mechanism, comprising:
a moving assembly (20);
the sampling assembly (10), the sampling assembly (10) includes a sampling needle (11) and a detection module (12), the sampling needle (11) is fixed on the moving assembly (20), the detection module (12) is electrically connected with the sampling needle (11), the change of the physical quantity of the sampling needle (11) is detected in real time, and the change is converted into an electric signal;
the driving unit (30) is coupled to the moving component (20) and drives the moving component (20) to move so as to drive the sampling needle (11) to move;
the control unit (40), it is connected with drive unit (30) electricity, sends drive control signal to drive unit (30), control unit (40) still is connected with detection module (12) electricity, gathers the signal of telecommunication from detection module (12), whether control unit (40) judge according to the comparative value of first signal of telecommunication and second signal of telecommunication and inhale the appearance volume and reach the predetermined amount, the signal of telecommunication that detection module (12) output before sampling needle (11) execution row appearance is first signal of telecommunication, the second signal of telecommunication is the signal of telecommunication that detection module (12) output after sampling needle (11) row appearance finishes.
2. The sampling mechanism according to claim 1, wherein the control unit (40) considers that the amount of the sucked sample has reached a predetermined amount when the comparison value is greater than or equal to a set threshold value, and otherwise considers that the amount of the sucked sample has not reached the predetermined amount.
3. A sampling mechanism, comprising:
a moving assembly (20);
the sampling assembly (10), the sampling assembly (10) includes a sampling needle (11) and a detection module (12), the sampling needle (11) is fixed on the moving assembly (20), the detection module (12) is electrically connected with the sampling needle (11), the change of the physical quantity of the sampling needle (11) is detected in real time, and the change is converted into an electric signal;
the driving unit (30) is coupled to the moving component (20) and drives the moving component (20) to move so as to drive the sampling needle (11) to move;
the control unit (40) is electrically connected with the driving unit (30) and sends a driving control signal to the driving unit (30), the control unit (40) is also electrically connected with the detection module (12) and collects an electric signal from the detection module (12), on one hand, the control unit (40) preliminarily judges whether the sample sucking quantity reaches a preset quantity according to a comparison value of a first electric signal and a second electric signal, on the other hand, the control unit preliminarily judges whether the sample sucking quantity reaches the preset quantity according to a comparison value of a third electric signal and a fourth electric signal and comprehensively judges whether the sample sucking quantity reaches the preset quantity according to results of two times of preliminary judgments, the first electric signal is an electric signal output by the detection module (12) before the sampling needle (11) executes sample discharge, the second electric signal is an electric signal output by the detection module (12) after the sampling needle (11) finishes sample discharge, and the third electric signal is an electric signal output by the detection module (12) before the sampling needle (11) executes sample sucking, and the fourth electric signal is an electric signal output by the detection module (12) after the sampling needle (11) finishes sample suction.
4. The sampling mechanism according to claim 3, wherein the control unit (40) considers that the amount of the sample sucked has not reached the predetermined amount when the amount of the sample sucked has not reached the predetermined amount for one of the two preliminary determinations, and considers that the amount of the sample sucked has reached the predetermined amount when the amount of the sample sucked has reached the predetermined amount for both of the two preliminary determinations.
5. The sampling mechanism of any one of claims 1-4, wherein the change in the physical quantity of the sampling pin is a change in capacitance of the sampling pin, and the electrical signal is a voltage signal.
6. The sampling mechanism according to any one of claims 1 to 4, wherein the first electrical signal is an electrical signal acquired by the control unit (40) from the detection module (12) after the sampling needle (11) has been moved to the reaction cup position and before the sample is placed, and the second electrical signal is an electrical signal acquired by the sampling needle (11) from the detection module (12) after the sample is placed at the sample placement position and the control unit (40) has completed the sample placement.
7. The sampling mechanism according to any one of claims 1 to 4, characterized in that the sampling needle (11) comprises double metal cylinders nested inside and outside, said double metal cylinders being insulated from each other and electrically connected to the detection module (12) by means of wires, respectively, said detection module (12) being fixed to the moving assembly (20); the sampling assembly (10) further comprises an injector (13), the output end of the injector (13) is communicated with the inner-layer needle cylinder of the sampling needle (11) through a pipeline, and the driving unit (30) is coupled to the pushing end of the injector (13) and drives the pushing end to move.
8. A method for detecting the suction of a sampling mechanism, wherein the sampling mechanism comprises a sampling needle (11) and a detection module (12), the detection module (12) is electrically connected with the sampling needle (11), and the detection module (12) detects the change of a physical quantity of the sampling needle (11) in real time and converts the change into an electric signal, and is characterized by comprising the following steps:
before the sampling needle (11) performs sample arrangement, the detection module (12) detects a physical quantity of the sampling needle (11) and outputs a first electric signal;
after the sampling of the sampling needle (11) is finished, the detection module (12) detects the physical quantity of the sampling needle (11) and outputs a second electric signal;
and judging whether the sample suction amount reaches a preset amount or not according to the comparison value of the first electric signal and the second electric signal.
9. A method for detecting the suction of a sampling mechanism, wherein the sampling mechanism comprises a sampling needle (11) and a detection module (12), the detection module (12) is electrically connected with the sampling needle (11), and the detection module (12) detects the change of a physical quantity of the sampling needle (11) in real time and converts the change into an electric signal, and is characterized by comprising the following steps:
before the sampling needle (11) performs sample suction, the detection module (12) detects the physical quantity of the sampling needle (11) and outputs a third electric signal;
after the sampling needle (11) finishes sample suction, the detection module (12) detects the physical quantity of the sampling needle and outputs a fourth electric signal;
preliminarily judging whether the sample suction amount reaches a preset amount according to a comparison value of the third electric signal and the fourth electric signal;
before the sampling needle (11) performs sample arrangement, the detection module (12) detects a physical quantity of the sampling needle (11) and outputs a first electric signal;
after the sampling of the sampling needle (11) is finished, the detection module (12) detects the physical quantity of the sampling needle (11) and outputs a second electric signal;
preliminarily judging whether the sample suction amount reaches a preset amount according to the comparison value of the first electric signal and the second electric signal;
and comprehensively judging whether the sample suction amount reaches a preset amount according to the results of the two primary judgments.
10. The method according to claim 8 or 9, wherein the change of the physical quantity of the sampling needle is a change of capacitance of the sampling needle, and the electrical signal is a voltage signal.
11. The method of claim 8 or 9,
the detection process of the first electric signal and the second electric signal comprises the following steps:
controlling the sampling needle (11) to move to the position of the reaction cup and stop above the reaction cup;
collecting a current electrical signal from the detection module (12) as a first electrical signal;
controlling the sampling needle (11) to move downwards to a stock layout position, and executing stock layout;
stopping for a predetermined time after the discharging is finished, and collecting the current electric signal from the detection module (12) as a second electric signal in the period.
12. A sample analyzer, characterized by comprising a sampling mechanism according to any one of claims 1 to 7.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2015/083976 WO2017008249A1 (en) | 2015-07-14 | 2015-07-14 | Sampling mechanism, skipped-vacuum testing method therefor, and sample analyzer |
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| Publication Number | Publication Date |
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| CN107709958A CN107709958A (en) | 2018-02-16 |
| CN107709958B true CN107709958B (en) | 2020-04-14 |
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| CN (1) | CN107709958B (en) |
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| CN109030097B (en) * | 2018-06-06 | 2023-04-18 | 迈克医疗电子有限公司 | Liquid taking test method and device |
| CN109030096A (en) * | 2018-06-06 | 2018-12-18 | 迈克医疗电子有限公司 | Take liquid test method and device |
| CN109030098A (en) * | 2018-06-06 | 2018-12-18 | 迈克医疗电子有限公司 | Take liquid test method and device |
| CN110221090B (en) * | 2019-06-21 | 2021-03-23 | 苏州长光华医生物医学工程有限公司 | Air suction and air discharge preventing system and method applying chemiluminescence analyzer |
| WO2021223168A1 (en) * | 2020-05-07 | 2021-11-11 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analyzer, sample analysis method, and computer-readable storage medium |
| CN111812343B (en) * | 2020-07-02 | 2023-03-03 | 桂林优利特医疗电子有限公司 | Liquid path system based on immunoturbidimetry measurement and control method thereof |
| CN114441279B (en) * | 2020-10-30 | 2024-07-05 | 深圳市瑞图生物技术有限公司 | Sample sampling and mixing device, mixing control method and sperm quality analyzer |
| CN114166830B (en) * | 2021-12-08 | 2023-07-14 | 苏州长光华医生物医学工程有限公司 | Sampling needle pipeline and sampling judgment method |
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| CN107709958A (en) | 2018-02-16 |
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