CN107699510A - The microorganism-decomposing agent and decomposed method of vegetable castoff and stalk - Google Patents
The microorganism-decomposing agent and decomposed method of vegetable castoff and stalk Download PDFInfo
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Abstract
The microorganism-decomposing agent of vegetable castoff and stalk of the present invention and decomposed method belong to the microorganism-decomposing technology for not producing stink when stalk quick composting decomposes.The microorganism dormancy state powder part by weight of this microorganism-decomposing agent is：25 parts of aspergillus niger；12 parts of the dendritic branch spore of bud；8 12 parts of rhodothece rubra；Sticky 8 12 parts of trichosporon cutaneum；6 12 parts of pichia pastoris phaff；27 32 parts of bacillus subtilis.Decomposed method and step：(1) microorganism-decomposing agent, (2) activation and dilution, (3) sprinkling microorganism-decomposing agent bacterium solution are prepared.Advantage：45 days wheat stalks rotten 70%, odorless；35 days maize straws rotten 70%, odorless；25 days rice straws rotten 70%, odorless；The substantially most of decomposition of 7 days vegetable castoffs, waste dish not under the influence of season vegetables farming, and industry odorless；Decomposition does not produce the odorous gas such as hydrogen sulfide, acetamide and ammonia.
Description
Technical field
The invention belongs to having disengaged from soil, it is impossible to which the vegetable castoff and stalk of regrowth carry out the skill of microorganism-decomposing
Art, the microorganism quick composting technology of stink is not produced during more particularly to vegetable castoff and straw decomposition.
Background technology
The annual crop waste stalk amount in China is huge, mainly cellulose (polysaccharide macromolecular material), hemicellulose,
In the seed omitted on a small quantity also have protein, straw degradative returning to the field can living resources recycle, again solve carelessly abandon or field
Between burn and cause the huge waste of environmental pollution.Stalk burns the severe haze weather brought without control, has seriously affected me
Normal life.
Chinese patent《Stalk putrid fermentation agent》201510574578.2 it is a kind of new zymophyte for being cultured out,
Its training method will celebrate curly hair yeast-like fungi, corn 50%, the fermented and cultured of water 50% to determine first；Secondly, trichoderma viride training
Support, take rotten wood powder 35%, wheat bran 50%, the fermented and cultured of water 15%；Finally, the culture of fungi, polygonum flaccidum 30% is added,
White sugar 30%, water 20%, fermented and cultured.
Chinese patent《Microorganism quick rotting agent and preparation method thereof》200710049161.X a kind of microorganism quick rotting agent, including
Saccharomycete and cellulose-decomposing bacteria, it is characterised in that the weight proportion of the microbial components is：30~60 parts of saccharomycete, fiber point
Solve 40~70 parts of bacterium.
Chinese patent《A kind of bacillus subtilis H4 and its decomposing microbial inoculum of preparation》It is 201410519541.5 of the invention public
The application of a kind of bacillus subtilis H4 and its decomposing microbial inoculum, decomposing microbial inoculum of preparation is opened, the decomposing microbial inoculum is by short bud
Spore bacillus H3 dormant states powder, bacillus subtilis H4 dormant states powder, bacillus subtilis H8-3 dormant states powder and starch are with weight
Than being formed for 1: 1: 1: 97；The present invention provides one plant of new strains -- bacillus subtilis H4, the decomposed bacterium prepared using the bacterial strain
Agent has the advantages of high temperature resistant, extracellular proteinase, lipase are with high-efficiency fermenting animal class discarded object, utilizes the decomposing microbial inoculum heap
Fertilizer has the advantages that environmental pollution is small, economy and facility, and the product after compost treatment passes through subsequent treatment, can be used as organic fertilizer
Reach the purpose to turn waste into wealth.
The content of the invention
It is an object of the invention to provide vegetable castoff and straw decomposition is accelerated, do not produce stink vegetable castoff and
The microorganism-decomposing agent of stalk, and its with the microorganism-decomposing agent of this vegetable castoff and stalk to vegetable castoff or stalk
Carry out the method that the quick composting of odorless decomposes.
The technical scheme is that：
The microorganism-decomposing agent of vegetable castoff and stalk, including the dendritic branch spore of bud, rhodothece rubra, sticky trichosporon cutaneum,
Pichia pastoris phaff and bacillus subtilis, it is characterised in that：Described dormant state powder microorganism wt ratio is：
The dendritic branch spore 1-2 parts of bud；Rhodothece rubra 8-12 parts；Sticky trichosporon cutaneum 8-12 parts；
Pichia pastoris phaff 6-12 parts；Bacillus subtilis 27-32 parts.
This decomposing agent is mainly used in vegetable castoff and stalk odorless quick composting decomposes.
Aspergillus niger (Aspergillus niger) can produce amylase, protease, cellulase, pectase, can decompose straw
Protein, cellulose, pectin in stalk.
The dendritic branch spore of bud (Cladosporium cladosporioides) produces cellulase, the fiber in decomposition stalk
Element and hemicellulose.
Rhodothece rubra (Rhodotorula rubra) produces acid protease, the protein in decomposition stalk.
Sticky trichosporon cutaneum (Trichosporon mucoides) produces alkali protease, the protein in decomposition stalk.
Pichia pastoris phaff (Pichia pastoris) produces neutral proteinase, the protein in decomposition stalk.
Bacillus subtilis (Bacillus subtilis) is produced based on pectase, also there is cellulase and protease, rotten
Solve based on the pectin in stalk.
Without mutual inhibitory action between various microorganisms in this microorganism-decomposing agent.
In the protein of vegetable castoff and stalk, there are acidic protein, alkaline protein, neutral protein, so
The bacterium for producing acid protease, the bacterium for producing alkali protease have been selected in this microorganism-decomposing agent, has produced neutral proteinase
Bacterium, make various protein can be by aerobic bacteria or the decomposition of the aerobic property disliked bacterium odorless.Both from these three generation protease
Purpose is the decomposition stalk for carrying out odorless.And select in the present invention bacterium for producing cellulose and produce the bacterium of pectase just
For the iuntercellular supporter in stalk, both the cellulose on cell membrane and interstitial were good by decomposition execution, lost stalk
Go three-dimensional shape profile and collapse, volume-diminished；Material molecule, which diminishes to be advantageous to quickly enter soil by rotten material, turns into fertilizer.
The reason for this decomposing agent can decompose to stalk odorless quick composting be：The bacterium that the present invention selects all be aerobic bacteria or
The aerobic property disliked bacterium, enzyme caused by these bacterium when vegetable castoff and straw decomposition to not producing hydrogen sulfide, acetamide and ammonia etc.
Odorous gas.So as to reach i.e. decomposition vegetable castoff and stalk, and the purpose of odorless.
The microorganism dormancy state powder part by weight of the microorganism-decomposing agent of preferred vegetable discarded object and stalk of the present invention is：
3 parts of aspergillus niger；1 part of the dendritic branch spore of bud；8 parts of rhodothece rubra；Sticky 8 parts of trichosporon cutaneum；
10 parts of pichia pastoris phaff；30 parts of bacillus subtilis.
The microorganism-decomposing agent of vegetable castoff and stalk carries out the method for fast rotting to it, it is characterised in that including with
Lower step：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
Aspergillus niger 2-5 parts；The dendritic branch spore 1-2 parts of bud；Rhodothece rubra 8-12 parts；Sticky trichosporon cutaneum 8-12 parts；
Pichia pastoris phaff 6-12 parts；Bacillus subtilis 27-32 parts；
In 1 gram of dormant state powder microorganism-decomposing agent, various active micro organism quantities are：
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution.
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 100-400 public affairs
On jin disengaging plant, the stalk that departs from soil, can not grow, it carries out microorganism-decomposing effect to stalk and is shown in Table 1.
Or 100 kilograms of microorganism-decomposing agent bacterium solutions be uniformly sprayed at 1000-2000 kilograms depart from plant, depart from soil,
On the vegetable castoff that can not be grown.It carries out microorganism-decomposing effect to vegetable castoff and is shown in Table 2.
To the decomposed effect table 1 of various stalk microbes
To the rotten effect table 2 of microorganism speed of various vegetable castoffs
To the explanation of table 1：
With in table 1,45 days wheat stalks of test group rot 70%, odorless；35 days maize straws rotten 70%, it is odorless
Taste；25 days rice straws rotten 70%, odorless.Control group begins with a small amount of stink for 35 days, lose within 60 days have it is rotten.
With in table 1, (1) test group：In stalk corn trials group, 35 days 1 gram in rotten maize straw, have 160,000,000
The aerobic and property disliked bacterium, there is 3,000,000 anaerobic bacterias.Due to it is most of be aerobic and the property disliked bacterium, fraction is anaerobic bacteria；Aerobic and suspicion oxygen
The property ripe stalk of mycocriny does not produce the odorous gas such as hydrogen sulfide, acetamide and ammonia；And the decomposed stalk production of fraction anaerobic bacteria
Raw hydrogen sulfide, acetamide and ammonia are few.So the purpose of the present invention is reached：Make wheat straw waste decomposition time more rotten than nature
Solve time quickening, and odorless or only a small amount of stink.
With in table 1, (2) control group：In a variety of mixed straws of control group, 35 days 1 gram in rotten maize straw, have 400
The ten thousand aerobic and property disliked bacterium, there is 12,200,000 anaerobic bacterias.Because major part is anaerobic bacteria, fraction is aerobic and the property disliked bacterium；Largely
Anaerobic bacteria produces hydrogen sulfide, acetamide and ammonia.
It can be seen that with table 1, the present invention has that obvious excellent quickening is decomposed than control group, and the technique effect of odorless.
To the explanation of table 2：
With in table 2, test group basically reaches most of decomposition for 7 days, waste dish not under the influence of season vegetables farming effect, and
Industry odorless；15 days substantially completely decompositions, and industry odorless of test group.Control group begins with decomposition in 7 days, there is a small amount of stink；It is right
Obvious decomposition is begun with according to group within 15 days, there is a small amount of stink；Control group begins with more than half decomposition in 30 days, there is dense stink.
With in table 2, (1) test group due to it is most of be aerobic and the property disliked bacterium, fraction is anaerobic bacteria；Aerobic and suspicion oxygen
The ripe stalk of mycocriny does not produce the odorous gas such as hydrogen sulfide, acetamide and ammonia；And the decomposed stalk of fraction anaerobic bacteria produces
Hydrogen sulfide, acetamide and ammonia are few.So the purpose of the present invention is reached：Make wheat straw waste decomposition time than nature decomposition
Time accelerates, and odorless or only a small amount of stink.
So that in table 2, (2) control group is because major part is anaerobic bacteria, and fraction is aerobic and the property disliked bacterium；Substantial amounts of anaerobic bacteria
Hydrogen sulfide, acetamide and ammonia are produced, produces stink.
It can be seen that with table 2, the present invention has that obvious excellent quickening is decomposed than control group, and the technique effect of odorless.
The various strains of the present invention are all purchased from Institute of Microorganism, Academia Sinica.
Each strain of product is made in each strain that the present invention is bought with following " level Four fermentation " technique respectively
Each strain dormant state powder is made in microorganism dormancy state powder, then the weight ratio these each strain dormant state powder by formula
It is well mixed, obtain the vegetable castoff of the present invention and the microorganism-decomposing agent of stalk.
" level Four fermentation " technique of each strain is as follows：
(1) the 1st grade of seed selection culture
In gnotobasis, the culture medium of agar slant will be accessed after a certain strain dormant state powder aquation bought
In, purified, after the inoculation of bacterial screening, test tube and Shaking culture through strain, bacterium solution is made by liquid fermentation.Described inclined-plane bacterium
The culture of kind：The inclined-plane culture of strain requires that slant strains must not be mixed with any miscellaneous bacteria and bacteriophage, is cultivated at 28 DEG C of constant temperature.
This seed culture is to select energetic thalline.
(2) the 2nd grades of seeds expand culture
1st grade of seed bacterium solution is expanded and cultivates the strain liquid that 1000-2000ml is made.
(3) 3rd level seeding tank fermented and cultured
This step is inoculated with the 2nd grade of zymocyte liquid according to 5% inoculum concentration, and 500L strain liquids are made.
(4) the 4th grades of fermentation tank cultures
3rd level zymocyte liquid is inoculated with according to 10% inoculum concentration in this step, 5000L zymotic fluid is made.Then by the 4th
Level zymotic fluid is dried to dormant state powder.
Advantages of the present invention：45 days wheat stalks rotten 70%, odorless；35 days maize straws rotten 70%, odorless；
25 days rice straws rotten 70%, odorless.It is to harvest waste dish 5-7 days rotten 70% discarded after vegetables to vegetable castoff,
Odorless, waste dish no longer under the influence of season vegetables farming；Substantially completely rot within 15 days.
From the aerobic and property the disliked bacterium that can be quickly bred in stalk, suppress the anaerobic bacteria in stalk, make vegetable castoff
Decomposed with stalk by the aerobic and property disliked bacterium quick composting, decomposition does not produce the odorous gas such as hydrogen sulfide, acetamide and ammonia.
The present invention is had from the aerobic of decomposition stalk and the property disliked bacterium：Produce the aspergillus niger of cellulase, the dendritic branch spore of bud, production
Aspergillus niger, rhodothece rubra, sticky trichosporon cutaneum and the pichia pastoris phaff of raw albumen enzyme, produce the bacillus subtilis of pectase
Bacterium and aspergillus niger coordinate.
Embodiment
Embodiment 1, the microorganism-decomposing agent of rice crop waste stalk carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is as follows：
(1) the 1st grade of seed selection culture：In gnotobasis, it will be accessed after a certain strain dormant state powder aquation bought
In the culture medium of agar slant, purify, after the inoculation of bacterial screening, test tube and Shaking culture through strain, be made up of liquid fermentation
Bacterium solution.The culture of described slant strains：The inclined-plane culture of strain requires that slant strains must not be mixed with any miscellaneous bacteria and bacteriophage,
Cultivated at 28 DEG C of constant temperature.This seed culture is to select energetic thalline.
(2) the 2nd grades of seeds expand culture：1st grade of seed bacterium solution is expanded and cultivates the strain liquid that 1000-2000ml is made.
(3) 3rd level seeding tank fermented and cultured：This step is inoculated with the 2nd grade of zymocyte liquid according to 5% inoculum concentration, is made
500L strain liquids.
(4) the 4th grades of fermentation tank cultures：3rd level zymocyte liquid is inoculated with according to 10% inoculum concentration in this step, is made
5000L zymotic fluid.Then the 4th grade of zymotic fluid is dried to dormant state powder.
The microorganism-decomposing agent and the method decomposed to rice straw progress of vegetable castoff and stalk, including following step
Suddenly：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
5 parts of aspergillus niger；1 part of the dendritic branch spore of bud；8 parts of rhodothece rubra；Sticky 8 parts of trichosporon cutaneum；
8 parts of pichia pastoris phaff；30 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 100-400 public affairs
Jin departs from the rice straw of plant.
Embodiment 2, the microorganism-decomposing agent of rice crop waste stalk carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to rice straw progress of vegetable castoff and stalk, including following step
Suddenly：(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, micro- life is made
Thing decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
3 parts of aspergillus niger；1 part of the dendritic branch spore of bud；8 parts of rhodothece rubra；Sticky 8 parts of trichosporon cutaneum；
6 parts of pichia pastoris phaff；27 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 100-400 public affairs
Jin departs from the rice straw of plant.
Embodiment 3, the microorganism-decomposing agent of maize farming thing waste straw carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to maize straw progress of vegetable castoff and stalk, including following step
Suddenly：(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, micro- life is made
Thing decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
2 parts of aspergillus niger；2 parts of the dendritic branch spore of bud；12 parts of rhodothece rubra；Sticky 12 parts of trichosporon cutaneum；
12 parts of pichia pastoris phaff；32 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 100-400 public affairs
Jin departs from the maize straw of plant.
Embodiment 4, the microorganism-decomposing agent of maize farming thing waste straw carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to maize straw progress of vegetable castoff and stalk, including following step
Suddenly：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
3 parts of aspergillus niger；1.5 parts of the dendritic branch spore of bud；10 parts of rhodothece rubra；Sticky 9 parts of trichosporon cutaneum；
10.0 parts of pichia pastoris phaff；30.0 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 100-400 public affairs
Jin departs from the maize straw of plant.
Embodiment 5, the microorganism-decomposing agent of wheat crop waste stalk carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to wheat stalk progress of vegetable castoff and stalk, including following step
Suddenly：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
2 parts of aspergillus niger；2 parts of the dendritic branch spore of bud；11 parts of rhodothece rubra；Sticky 10 parts of trichosporon cutaneum；
8 parts of pichia pastoris phaff；28 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 100-400 public affairs
Jin departs from the wheat stalk of plant.
Embodiment 6, the microorganism-decomposing agent of wheat crop waste stalk carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to wheat stalk progress of vegetable castoff and stalk, including following step
Suddenly：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
3 parts of aspergillus niger；2 parts of the dendritic branch spore of bud；10 parts of rhodothece rubra；Sticky 12 parts of trichosporon cutaneum；
7 parts of pichia pastoris phaff；29 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 100-400 public affairs
Jin departs from the wheat stalk of plant.
Embodiment 7, the microorganism-decomposing agent of pumpkin discarded object waste dish carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to the progress of pumpkin discarded object of vegetable castoff and stalk, including following step
Suddenly：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
2 parts of aspergillus niger；1 part of the dendritic branch spore of bud；10 parts of rhodothece rubra；Sticky 10 parts of trichosporon cutaneum；
7 parts of pichia pastoris phaff；29 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 1000-2000
Kilogram depart from plant pumpkin discarded object on.
Embodiment 8, the microorganism-decomposing agent of lotus rhizome discarded object waste dish carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to the progress of lotus rhizome discarded object of vegetable castoff and stalk, including following step
Suddenly：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
2 parts of aspergillus niger；1 part of the dendritic branch spore of bud；12 parts of rhodothece rubra；Sticky 10 parts of trichosporon cutaneum；
9 parts of pichia pastoris phaff；28 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 1000-2000
Kilogram depart from plant lotus rhizome discarded object on.
Embodiment 9, the microorganism-decomposing agent of broad bean discarded object waste dish carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to the progress of broad bean discarded object of vegetable castoff and stalk, including following step
Suddenly：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
2 parts of aspergillus niger；2 parts of the dendritic branch spore of bud；12 parts of rhodothece rubra；Sticky 10 parts of trichosporon cutaneum；
11 parts of pichia pastoris phaff；30 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 1000-2000
Kilogram depart from plant broad bean discarded object on.
Embodiment 10, the microorganism-decomposing agent of tomato discarded object waste dish carry out decomposed method
Prepare every kind of microorganism dormancy state powder respectively first, then every kind of microorganism dormancy state powder is mixed by formula rate and made
Obtain the microorganism-decomposing agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism-decomposing agent and the method decomposed to the progress of tomato discarded object of vegetable castoff and stalk, including it is following
Step：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, are made
Microorganism-decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
2 parts of aspergillus niger；2 parts of the dendritic branch spore of bud；11 parts of rhodothece rubra；Sticky 11 parts of trichosporon cutaneum；
11 parts of pichia pastoris phaff；30 parts of bacillus subtilis.
(2) activate and dilute：500ml water is added to be slowly stirred under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
Mixing 24 hours, is made microorganism-decomposing agent activating solution；Activated microorganism-decomposing agent activating solution is diluted with 100 kilograms again
Into microorganism-decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 1000-2000
Kilogram depart from plant tomato discarded object on.
Claims (3)
1. the microorganism-decomposing agent of vegetable castoff and stalk, including the dendritic branch spore of aspergillus niger, bud, rhodothece rubra, sticky silk spore
Yeast, pichia pastoris phaff and bacillus subtilis, it is characterised in that：Described dormant state powder microorganism wt ratio is：
Aspergillus niger 2-5 parts；The dendritic branch spore 1-2 parts of bud；Rhodothece rubra 8-12 parts；Sticky trichosporon cutaneum 8-12 parts；
Pichia pastoris phaff 6-12 parts；Bacillus subtilis 27-32 parts.
2. the microorganism-decomposing agent of vegetable castoff according to claim 1 and stalk, it is characterised in that：Described dormancy
State powder microorganism wt ratio is：
3 parts of aspergillus niger；1 part of the dendritic branch spore of bud；8 parts of rhodothece rubra；Sticky 8 parts of trichosporon cutaneum；
10 parts of pichia pastoris phaff；30 parts of bacillus subtilis.
3. decomposed method is carried out to it with the vegetable castoff described in claim 1 or 2 and the microorganism-decomposing agent of stalk, its
It is characterised by comprising the following steps：
(1) microorganism-decomposing agent is prepared：Various microorganism dormancy state powder are well mixed by the part by weight of formula, micro- life is made
Thing decomposing agent, the part by weight of its various microorganism dormancy state powder formula be,
Aspergillus niger 2-5 parts；The dendritic branch spore 1-2 parts of bud；Rhodothece rubra 8-12 parts；Sticky trichosporon cutaneum 8-12 parts；
Pichia pastoris phaff 6-12 parts；Bacillus subtilis 27-32 parts；
(2) activate and dilute：500ml water is added to be slowly stirred mixing under 25-35 degrees celsius 5.0 grams of microorganism-decomposing agents
24 hours, microorganism-decomposing agent activating solution is made；Activated microorganism-decomposing agent activating solution is diluted to 100 kilograms again micro-
Biological decomposing agent bacterium solution；
(3) microorganism-decomposing agent bacterium solution is sprayed：100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at 100-400 kilograms to take off
From on the stalk of plant；Or 100 kilograms of microorganism-decomposing agent bacterium solutions are uniformly sprayed at the vegetable of 1000-2000 kilograms of disengaging plant
On dish discarded object.
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| CN1454191A (en) * | 2000-09-05 | 2003-11-05 | 欧亚生物科技有限公司 | A biological fertilizer based on yeasts |
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