CN107653214A - A kind of photosynthetic microbial inoculum cultural method - Google Patents
A kind of photosynthetic microbial inoculum cultural method Download PDFInfo
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- CN107653214A CN107653214A CN201711136974.2A CN201711136974A CN107653214A CN 107653214 A CN107653214 A CN 107653214A CN 201711136974 A CN201711136974 A CN 201711136974A CN 107653214 A CN107653214 A CN 107653214A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of photosynthetic microbial inoculum cultural method, comprise the following steps：A. prepared by culture medium：It is in alkalescent by pure water modulation, pH value is 7.5 8.5, and according to addition peptone 0.3%, sodium citrate 0.3%, yeast extract 0.2% in mass percent pure water, sodium acid carbonate 0.2%, sodium chloride 0.2%, ammonium chloride 0.2%, epsom salt 0.2%, potassium dihydrogen phosphate 0.2%, surplus are pure water；B. strain is selected：From effective viable bacteria kind, and the strain includes Rhodopseudomonas spheroides and Rhodopseudomonas palustris, according to mass percent 1:1.5 mixing；Wherein Rhodopseudomonas palustris is cultivated to red single bacterium colony by double-layer plate cultivation and occurred；C. seed liquor culture：Mixed live kind obtained by above-mentioned step B is subjected to fermented and cultured, the temperature of culture is 27 32 DEG C, and illumination condition is 2500Lx~3000Lx；The culture efficiency of photosynthetic bacteria microbial inoculum can be effectively improved using the present invention.
Description
Technical field
The present invention relates to technical field of valve manufacturing, more particularly to a kind of photosynthetic microbial inoculum cultural method.
Background technology
Photosynthetic bacteria (abbreviation PSB) is that occur generally existing in earliest, nature on the earth, have original luminous energy compound body
The prokaryotes of system, it is the general name for not put the photosynthetic bacterium of oxygen under anaerobic, is that one kind does not form gemma
The Gram-negative bacteria of ability, be one kind using light as the energy, nature can be utilized under anaerobism illumination or aerobic dark condition
In organic matter, sulfide, ammonia etc. carry out photosynthetic microorganism as hydrogen donor and carbon source.Photosynthetic bacteria is distributed widely in
The soil of nature, paddy field, marsh, lake, Jiang Hai etc., are distributed mainly on the anoxic that light can be transmitted in aquatic environment
Area.
And the efficiency of photosynthetic bacteria microbial inoculum and its low, it is necessary to improve the culture efficiency of microbial inoculum is formed naturally.
The content of the invention
The present invention solves the technical problem of a kind of photosynthetic microbial inoculum cultural method is provided, photosynthetic bacteria microbial inoculum is improved
Culture efficiency.
To solve above-mentioned technical purpose, the technical solution adopted by the present invention is as follows：
A kind of photosynthetic microbial inoculum cultural method, it is characterised in that comprise the following steps：
A. prepared by culture medium：It is in alkalescent that pure water, which is modulated, pH value 7.5-8.5, and pure according to mass percent
Peptone 0.3%, sodium citrate 0.3%, yeast extract 0.2%, sodium acid carbonate 0.2%, sodium chloride 0.2%, chlorination are added in water
Ammonium 0.2%, epsom salt 0.2%, potassium dihydrogen phosphate 0.2%, surplus are pure water；
B. strain is selected：From effective viable bacteria kind, and the strain includes Rhodopseudomonas spheroides and Rhodopseudomonas palustris,
According to mass percent 1:1.5 mixing；Wherein Rhodopseudomonas palustris is cultivated to red single bacterium by double-layer plate cultivation
Drop out existing；
C. seed liquor culture：Mixed live kind obtained by above-mentioned step B is subjected to fermented and cultured, the temperature of culture is 27-
32 DEG C, illumination condition is 2500Lx~3000Lx.
Further, in the step C, fermentation medium need to be added, the fermentation medium adds in every 100ml pure water
Enter potassium propionate 0.2g, ammonium chloride 0.1g, yeast extract 0.2g, vitamin 0.1g.
The beneficial effects of the invention are as follows：The situation of prior art is different from, the made photosynthetic microbial inoculum of the present invention is more efficient, matter
Amount is more preferable.
Embodiment
The present invention is described further with reference to specific embodiment.
A kind of photosynthetic microbial inoculum cultural method, it is characterised in that comprise the following steps：
A. prepared by culture medium：It is in alkalescent that pure water, which is modulated, pH value 7.5-8.5, and pure according to mass percent
Peptone 0.3%, sodium citrate 0.3%, yeast extract 0.2%, sodium acid carbonate 0.2%, sodium chloride 0.2%, chlorination are added in water
Ammonium 0.2%, epsom salt 0.2%, potassium dihydrogen phosphate 0.2%, surplus are pure water；
B. strain is selected：From effective viable bacteria kind, and the strain includes Rhodopseudomonas spheroides and Rhodopseudomonas palustris,
According to mass percent 1:1.5 mixing；Wherein Rhodopseudomonas palustris is cultivated to red single bacterium by double-layer plate cultivation
Drop out existing；
C. seed liquor culture：Mixed live kind obtained by above-mentioned step B is subjected to fermented and cultured, the temperature of culture is 27-
32 DEG C, illumination condition is 2500Lx~3000Lx；
The made photosynthetic microbial inoculum of the present invention is more efficient, better quality.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize invention
The equivalent structure or equivalent flow conversion that description is made, or other related technical areas are directly or indirectly used in,
It is included within the scope of the present invention.
Claims (2)
1. a kind of photosynthetic microbial inoculum cultural method, it is characterised in that comprise the following steps：
A. prepared by culture medium：It is in alkalescent that pure water, which is modulated, pH value 7.5-8.5, and according in mass percent pure water
Add peptone 0.3%, sodium citrate 0.3%, yeast extract 0.2%, sodium acid carbonate 0.2%, sodium chloride 0.2%, ammonium chloride
0.2%, epsom salt 0.2%, potassium dihydrogen phosphate 0.2%, surplus is pure water；
B. strain is selected：From effective viable bacteria kind, and the strain includes Rhodopseudomonas spheroides and Rhodopseudomonas palustris, according to
Mass percent 1:1.5 mixing；Wherein Rhodopseudomonas palustris is cultivated to red single bacterium by double-layer plate cultivation and dropped out
It is existing；
C. seed liquor culture：Mixed live kind obtained by above-mentioned step B is subjected to fermented and cultured, the temperature of culture is 27-32 DEG C,
Illumination condition is 2500Lx~3000Lx.
2. photosynthetic microbial inoculum cultural method according to claim 1, it is characterised in that in the step C, fermentation training need to be added
Base is supported, the fermentation medium adds potassium propionate 0.2g, ammonium chloride 0.1g, yeast extract 0.2g, vitamin in every 100ml pure water
0.1g。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711136974.2A CN107653214A (en) | 2017-11-16 | 2017-11-16 | A kind of photosynthetic microbial inoculum cultural method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711136974.2A CN107653214A (en) | 2017-11-16 | 2017-11-16 | A kind of photosynthetic microbial inoculum cultural method |
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| CN201711136974.2A Withdrawn CN107653214A (en) | 2017-11-16 | 2017-11-16 | A kind of photosynthetic microbial inoculum cultural method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220203A (en) * | 2018-03-06 | 2018-06-29 | 上海海洋大学 | A kind of fermentation medium of hydrogenlike silicon ion |
| CN111321091A (en) * | 2020-01-18 | 2020-06-23 | 平少华 | Basic original culture medium formula for starting composite photodynamic bacterium agent production system |
-
2017
- 2017-11-16 CN CN201711136974.2A patent/CN107653214A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220203A (en) * | 2018-03-06 | 2018-06-29 | 上海海洋大学 | A kind of fermentation medium of hydrogenlike silicon ion |
| CN108220203B (en) * | 2018-03-06 | 2021-03-02 | 上海海洋大学 | Fermentation medium of rhodobacter sphaeroides and application of fermentation medium in fermentation production of rhodobacter sphaeroides |
| CN111321091A (en) * | 2020-01-18 | 2020-06-23 | 平少华 | Basic original culture medium formula for starting composite photodynamic bacterium agent production system |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180202 |
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| WW01 | Invention patent application withdrawn after publication |