CN111321091A - Basic original culture medium formula for starting composite photodynamic bacterium agent production system - Google Patents
Basic original culture medium formula for starting composite photodynamic bacterium agent production system Download PDFInfo
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- CN111321091A CN111321091A CN202010058668.7A CN202010058668A CN111321091A CN 111321091 A CN111321091 A CN 111321091A CN 202010058668 A CN202010058668 A CN 202010058668A CN 111321091 A CN111321091 A CN 111321091A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a basic original culture medium formula for starting a composite photodynamic bacterium agent production system, which comprises the following raw materials in parts by weight: 3-7 parts of sodium chloride, 3-7 parts of ammonium chloride, 3-7 parts of sodium bicarbonate, 13-17 parts of sodium acetate, 5.5-9.5 parts of monopotassium phosphate, 0.5-1.5 parts of sodium hydroxide and VB120.3-0.7 part of yeast extract, 0.5-1.5 parts of magnesium sulfate, 8-12 parts of peptone, 8-12 parts of yeast extract, 1200 parts of aqueous solution and 1200 parts of photodynamic original flora. The invention achieves the aim of meeting the nutrition required by the growth of the photodynamic original flora, leads the photodynamic original flora in the culture medium to grow rapidly, simultaneously effectively inhibits the growth of mixed bacteria and harmful flora, and ensures the growth of complex bacteriaThe photodynamic microecological balance flora is healthy and continuously grown, the growth period of the original flora is reduced, the cultivation of planting personnel is facilitated, and the structure of soil is changed, so that the supply of plant nutrients is increased, the plant growth is promoted, the yield is increased, and the quality of agricultural products and the agricultural ecological environment are improved.
Description
Technical Field
The invention relates to the technical field of planting equipment, in particular to a basic original culture medium formula for starting a composite photodynamic bacterium agent production system.
Background
The source of modern human food is dependent on agricultural production as the first industry. Due to the explosive growth of the world population, the supply of nutrients by soil base fertility cannot produce enough food to meet the needs of human beings. Therefore, in agricultural production, a large amount of fertilizer must be applied, and the fertilizer is strategic material for guaranteeing national food safety and is a material basis for maintaining and improving land capability and realizing agricultural sustainable development. The fertilizer can be divided into organic fertilizer, inorganic fertilizer, biological fertilizer and other types according to the components. The organic fertilizer contains a large amount of organic substances and various complete nutrients, increases the content of organic matters in soil by applying the organic fertilizer, can improve the physical, chemical and biological characteristics of the soil, fertilizes the soil, and improves the quality of crops. However, due to the application of a large amount of chemical fertilizers, inorganic elements enriched in soil are increased, organic matters and beneficial microorganisms are rapidly gathered and reduced, so that the soil is hardened, the quality is reduced, the water and fertilizer retention capacity is reduced, agricultural products are seriously polluted, the ecological environment of the whole farmland and the living environment of people are affected, and how to maintain the sustainable development of agriculture in China has attracted high attention of governments at all levels.
The microbial fertilizer is also called microbial inoculum or bacterial fertilizer and mainly comprises beneficial microbes, an organic carrier, an inorganic fertilizer and the like. In recent years, the microbial fertilizer has great advantages in promoting the growth of crops, improving the disease resistance and stress resistance of crops, increasing the soil fertility and the like, and is regarded as important. The biological fertilizer is a product containing specific microorganism living bodies, is applied to agricultural production, and can increase the supply of plant nutrients or promote the growth of plants, increase the yield, and improve the quality of agricultural products and the agricultural ecological environment through the life activities of the microorganisms contained in the biological fertilizer.
The application of biofertilizer is very common, but as the strains are complex during culture, the content of effective bacteria is low during culture, the content of mixed bacteria exceeds the standard, and the like, and as the growers do not know the technology, and also can add an improper amount of chemical fertilizer or other additives into the biofertilizer, the content of nitrogen, phosphorus and potassium in the chemical fertilizer in the biofertilizer is too high, so that the effective bacteria die, the nutrition required by the growth of the photodynamic original flora cannot be met, the rapid propagation and the continuous growth of the composite photodynamic flora cannot be ensured, the effect of the biofertilizer is lost, the crop planting is caused by plant diseases and insect pests, and the like, and the crop yield is reduced, so the improvement is needed.
Disclosure of Invention
The invention aims to solve the defects that the original photodynamic bacterium flora grows slowly and is easy to generate mixed bacteria due to unbalanced nutrition added when the original photodynamic bacterium flora is just cultured, and provides a basic original culture medium formula for starting a composite photodynamic bacterium agent production system.
In order to achieve the purpose, the invention adopts the following technical scheme:
a basic original culture medium formula for starting a composite photodynamic bacterium agent production system comprises the following raw materials in parts by weight: 3-7 parts of sodium chloride, 3-7 parts of ammonium chloride, 3-7 parts of sodium bicarbonate, 13-17 parts of sodium acetate, 5.5-9.5 parts of monopotassium phosphate, 0.5-1.5 parts of sodium hydroxide and VB120.3-0.7 part of yeast extract, 0.5-1.5 parts of magnesium sulfate, 8-12 parts of peptone, 8-12 parts of yeast extract, 1200 parts of aqueous solution and 1200 parts of photodynamic original flora.
Preferably, the culture medium comprises the following raw materials in parts by weight: 4-6 parts of sodium chloride, 4-6 parts of ammonium chloride, 4-6 parts of sodium bicarbonate, 14-16 parts of sodium acetate, 6.5-8.5 parts of monopotassium phosphate, 0.75-1.25 parts of sodium hydroxide and VB120.4-0.6 part, 0.75-1.25 parts of magnesium sulfate, 9-11 parts of peptone, 9-11 parts of yeast extract, 900 parts of aqueous solution and 1100 parts of photodynamic original flora.
Preferably, the culture medium comprises the following raw materials in parts by weight: 5 parts of sodium chloride, 5 parts of ammonium chloride, 5 parts of sodium bicarbonate, 15 parts of sodium acetate, 7.5 parts of monopotassium phosphate, 1 part of sodium hydroxide and VB120.5 part, 1 part of magnesium sulfate, 10 parts of peptone, 10 parts of yeast extract, 1000 parts of aqueous solution and photodynamic original flora.
Preferably, said photodynamic primary population comprises: rhodopseudomonas palustris, Rhodopseudomonas haemolytica, Rhodopseudomonas sphaeroides, Rhodopseudomonas rhodochrous, Pseudomonas azotoformis, Rhodopseudomonas thiophilic, and Rhodopseudomonas viridis.
Preferably, the concentration of the cells of the photodynamic protobacteria is 100 hundred million cf/ml.
Preferably, the aqueous solution is purified water.
Preferably, the temperature of the medium is 20-40 ℃.
Preferably, the pH value of the culture medium is 7.0-7.5.
Compared with the prior art, the invention has the beneficial effects that: the invention achieves the purpose of meeting the nutrition required by the growth of the photodynamic original flora, enables the photodynamic original flora in the culture medium to grow rapidly, simultaneously effectively inhibits the growth of mixed bacteria and harmful flora, ensures the healthy and continuous growth of the composite photodynamic microecological balance flora, reduces the growth period of the original flora, facilitates the culture of planting personnel, changes the structure of soil, thereby increasing the supply of plant nutrients, promoting the growth of plants, improving the yield, and improving the quality of agricultural products and the agricultural ecological environment.
Detailed Description
The present invention will be further illustrated by the following examples, which are intended to be merely illustrative and not limitative.
Example 1
A basic original culture medium formula for starting a composite photodynamic bacterium agent production system comprises the following raw materials in parts by weight: 3 parts of sodium chloride, 3 parts of ammonium chloride, 3 parts of sodium bicarbonate, 13 parts of sodium acetate, 5.5 parts of monopotassium phosphate, 0.5 part of sodium hydroxide and VB120.3 part, 0.5 part of magnesium sulfate, 8 parts of peptone, 8 parts of yeast extract, 800 parts of aqueous solution and photodynamic original flora.
In the present invention, the original population of photodynamic bacteria comprises: rhodopseudomonas palustris, Rhodopseudomonas haemolytica, Rhodopseudomonas sphaeroides, Rhodopseudomonas rhodochrous, Pseudomonas azotoformis, Rhodopseudomonas thiophilic, and Rhodopseudomonas viridis.
Example 2
A basic original culture medium formula for starting a composite photodynamic bacterium agent production system comprises the following raw materials in parts by weight: 4 parts of sodium chloride, 4 parts of ammonium chloride, 4 parts of sodium bicarbonate, 14 parts of sodium acetate, 6.5 parts of potassium dihydrogen phosphate, 0.75 part of sodium hydroxide and VB120.4 part, 0.75 part of magnesium sulfate, 9 parts of peptone, 9 parts of yeast extract, 900 parts of aqueous solution and photodynamic original flora.
In the present invention, the original population of photodynamic bacteria comprises: rhodopseudomonas palustris, Rhodopseudomonas haemolytica, Rhodopseudomonas sphaeroides, Rhodopseudomonas rhodochrous, Pseudomonas azotoformis, Rhodopseudomonas thiophilic, and Rhodopseudomonas viridis.
Example 3
A basic original culture medium formula for starting a composite photodynamic bacterium agent production system comprises the following raw materials in parts by weight: 5 parts of sodium chloride, 5 parts of ammonium chloride, 5 parts of sodium bicarbonate, 15 parts of sodium acetate, 7.5 parts of monopotassium phosphate, 1 part of sodium hydroxide and VB120.5 part, 1 part of magnesium sulfate, 10 parts of peptone, 10 parts of yeast extract, 1000 parts of aqueous solution and photodynamic original flora.
In the present invention, the original population of photodynamic bacteria comprises: rhodopseudomonas palustris, Rhodopseudomonas haemolytica, Rhodopseudomonas sphaeroides, Rhodopseudomonas rhodochrous, Pseudomonas azotoformis, Rhodopseudomonas thiophilic, and Rhodopseudomonas viridis.
Example 4
A basic original culture medium formula for starting a composite photodynamic bacterium agent production system comprises the following raw materials in parts by weight: 6 parts of sodium chloride, 6 parts of ammonium chloride, 6 parts of sodium bicarbonate, 16 parts of sodium acetate, 8.5 parts of monopotassium phosphate, 1.25 parts of sodium hydroxide and VB120.6 part, 1.25 parts of magnesium sulfate, 11 parts of peptone, 11 parts of yeast extract, 1100 parts of aqueous solution and photodynamic original flora.
In the present invention, the original population of photodynamic bacteria comprises: rhodopseudomonas palustris, Rhodopseudomonas haemolytica, Rhodopseudomonas sphaeroides, Rhodopseudomonas rhodochrous, Pseudomonas azotoformis, Rhodopseudomonas thiophilic, and Rhodopseudomonas viridis.
Example 5
A basic original culture medium formula for starting a composite photodynamic bacterium agent production system comprises the following raw materials in parts by weight: 7 parts of sodium chloride, 7 parts of ammonium chloride, 7 parts of sodium bicarbonate, 17 parts of sodium acetate, 9.5 parts of monopotassium phosphate, 1.5 parts of sodium hydroxide and VB120.7 part, 1.5 parts of magnesium sulfate, 12 parts of peptone, 12 parts of yeast extract, 1200 parts of aqueous solution and photodynamic original flora.
In the present invention, the original population of photodynamic bacteria comprises: rhodopseudomonas palustris, Rhodopseudomonas haemolytica, Rhodopseudomonas sphaeroides, Rhodopseudomonas rhodochrous, Pseudomonas azotoformis, Rhodopseudomonas thiophilic, and Rhodopseudomonas viridis.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. A basic original culture medium formula for starting a composite photodynamic bacterium agent production system is characterized in that a culture medium comprises the following raw materials in parts by weight: 3-7 parts of sodium chloride, 3-7 parts of ammonium chloride, 3-7 parts of sodium bicarbonate, 13-17 parts of sodium acetate, 5.5-9.5 parts of monopotassium phosphate, 0.5-1.5 parts of sodium hydroxide and VB120.3-0.7 part of yeast extract, 0.5-1.5 parts of magnesium sulfate, 8-12 parts of peptone, 8-12 parts of yeast extract, 1200 parts of aqueous solution and 1200 parts of photodynamic original flora.
2. The basic original culture medium formula for starting the composite photodynamic bacteria agent production system as claimed in claim 1, wherein the culture medium comprises the following raw materials in parts by weight: 4-6 parts of sodium chloride, 4-6 parts of ammonium chloride, 4-6 parts of sodium bicarbonate, 14-16 parts of sodium acetate, 6.5-8.5 parts of monopotassium phosphate, 0.75-1.25 parts of sodium hydroxide and VB120.4-0.6 part, 0.75-1.25 parts of magnesium sulfate, 9-11 parts of peptone, 9-11 parts of yeast extract, 900 parts of aqueous solution and 1100 parts of photodynamic original flora.
3. The basic original culture medium formula for starting the composite photodynamic bacteria agent production system as claimed in claim 1, wherein the culture medium comprises the following raw materials in parts by weight: 5 parts of sodium chloride, 5 parts of ammonium chloride, 5 parts of sodium bicarbonate, 15 parts of sodium acetate, 7.5 parts of monopotassium phosphate, 1 part of sodium hydroxide and VB120.5 part, 1 part of magnesium sulfate, 10 parts of peptone, 10 parts of yeast extract, 1000 parts of aqueous solution and photodynamic original flora.
4. The basic original culture medium formula for starting the composite photodynamic bacterial agent production system according to claim 1, wherein the photodynamic original bacterial flora comprises: rhodopseudomonas palustris, Rhodopseudomonas haemolytica, Rhodopseudomonas sphaeroides, Rhodopseudomonas rhodochrous, Pseudomonas azotoformis, Rhodopseudomonas thiophilic, and Rhodopseudomonas viridis.
5. The basic original culture medium formula for starting the composite photodynamic bacterium agent production system as claimed in claim 1, wherein the cell concentration of the photodynamic original bacteria is 100 hundred million cf/ml.
6. The basic original culture medium formula for starting the composite photodynamic bacterium agent production system as claimed in claim 1, wherein the aqueous solution adopts purified water.
7. The basic original culture medium formula for starting the composite photodynamic bacterial agent production system according to claim 1, wherein the temperature of the culture medium is 20-40 ℃.
8. The basic original culture medium formula for starting the composite photodynamic bacterial agent production system as claimed in claim 1, wherein the pH value of the culture medium is 7.0-7.5.
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| CN202010058668.7A CN111321091A (en) | 2020-01-18 | 2020-01-18 | Basic original culture medium formula for starting composite photodynamic bacterium agent production system |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6982161B1 (en) * | 2003-10-15 | 2006-01-03 | Markus Donald Herrema | Process for the utilization of ruminant animal methane emissions |
| CN107653214A (en) * | 2017-11-16 | 2018-02-02 | 成都市新津胤春生物科技有限责任公司 | A kind of photosynthetic microbial inoculum cultural method |
| CN108587975A (en) * | 2018-05-16 | 2018-09-28 | 广西青又青生物肥业有限公司 | A kind of Rhodopseudomonas palustris culture medium and cultural method |
-
2020
- 2020-01-18 CN CN202010058668.7A patent/CN111321091A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6982161B1 (en) * | 2003-10-15 | 2006-01-03 | Markus Donald Herrema | Process for the utilization of ruminant animal methane emissions |
| CN107653214A (en) * | 2017-11-16 | 2018-02-02 | 成都市新津胤春生物科技有限责任公司 | A kind of photosynthetic microbial inoculum cultural method |
| CN108587975A (en) * | 2018-05-16 | 2018-09-28 | 广西青又青生物肥业有限公司 | A kind of Rhodopseudomonas palustris culture medium and cultural method |
Non-Patent Citations (3)
| Title |
|---|
| 姜成林: "微生物资源开发利用", 中国轻工业出版社, pages: 128 * |
| 张美昭: "《无公害鲆鲽类标准化生产》", 31 January 2006, pages: 39 * |
| 王玉堂: "《有益微生物制剂及其在水产养殖中的应用》", 30 September 2018, pages: 149 * |
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