CN107653211A - A kind of photosynthetic bacterial preparation process - Google Patents
A kind of photosynthetic bacterial preparation process Download PDFInfo
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- CN107653211A CN107653211A CN201711136123.8A CN201711136123A CN107653211A CN 107653211 A CN107653211 A CN 107653211A CN 201711136123 A CN201711136123 A CN 201711136123A CN 107653211 A CN107653211 A CN 107653211A
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- pure water
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of photosynthetic bacterial preparation process, comprise the following steps：A. prepared by culture medium：It is in alkalescent by pure water modulation, pH value is 7.5 8.5, and according to addition peptone 0.3%, sodium citrate 0.3%, yeast extract 0.2% in mass percent pure water, sodium acid carbonate 0.2%, sodium chloride 0.2%, ammonium chloride 0.2%, epsom salt 0.2%, potassium dihydrogen phosphate 0.2%, surplus are pure water；B. actication of culture：Using sealing container as incubator, fill the culture medium obtained by step A and be inoculated in the 70% of volume, and by photosynthetic bacteria strain in culture medium, 27 32 DEG C of cultivation temperature, the 120h of incubation time 100, obtain activated spawn；C. seed liquor culture：Activated spawn obtained by above-mentioned step B is subjected to fermented and cultured, the temperature of culture is 27 32 DEG C, and illumination condition is 2500Lx~3000Lx；The culture efficiency of photosynthetic bacteria microbial inoculum can be effectively improved using the present invention.
Description
Technical field
The present invention relates to technical field of valve manufacturing, more particularly to a kind of photosynthetic bacterial preparation process.
Background technology
Photosynthetic bacteria (abbreviation PSB) is that occur generally existing in earliest, nature on the earth, have original luminous energy compound body
The prokaryotes of system, it is the general name for not put the photosynthetic bacterium of oxygen under anaerobic, is that one kind does not form gemma
The Gram-negative bacteria of ability, be one kind using light as the energy, nature can be utilized under anaerobism illumination or aerobic dark condition
In organic matter, sulfide, ammonia etc. carry out photosynthetic microorganism as hydrogen donor and carbon source.Photosynthetic bacteria is distributed widely in
The soil of nature, paddy field, marsh, lake, Jiang Hai etc., are distributed mainly on the anoxic that light can be transmitted in aquatic environment
Area.
And the efficiency of photosynthetic bacteria microbial inoculum and its low, it is necessary to improve the culture efficiency of microbial inoculum is formed naturally.
The content of the invention
The present invention solves the technical problem of a kind of photosynthetic bacterial preparation process is provided, photosynthetic bacteria microbial inoculum is improved
Culture efficiency.
To solve above-mentioned technical purpose, the technical solution adopted by the present invention is as follows：
A kind of photosynthetic bacterial preparation process, it is characterised in that comprise the following steps：
A. prepared by culture medium：It is in alkalescent that pure water, which is modulated, pH value 7.5-8.5, and pure according to mass percent
Peptone 0.3%, sodium citrate 0.3%, yeast extract 0.2%, sodium acid carbonate 0.2%, sodium chloride 0.2%, chlorination are added in water
Ammonium 0.2%, epsom salt 0.2%, potassium dihydrogen phosphate 0.2%, surplus are pure water；
B. actication of culture：Using sealing container as incubator, culture medium obtained by step A is filled to the 70% of volume,
And photosynthetic bacteria strain is inoculated in culture medium, 27-32 DEG C of cultivation temperature, incubation time 100-120h, obtain activated spawn；
C. seed liquor culture：Activated spawn obtained by above-mentioned step B is subjected to fermented and cultured, the temperature of culture is 27-32
DEG C, illumination condition is 2500Lx~3000Lx；
Further, in the step C, fermentation medium need to be added, the fermentation medium adds in every 100ml pure water
Enter potassium propionate 0.2g, ammonium chloride 0.1g, yeast extract 0.2g, vitamin 0.1g.
The beneficial effects of the invention are as follows：The situation of prior art is different from, the made photosynthetic microbial inoculum of the present invention is more efficient, matter
Amount is more preferable.
Embodiment
The present invention is described further with reference to specific embodiment.
A kind of photosynthetic bacterial preparation process, it is characterised in that comprise the following steps：
A. prepared by culture medium：It is in alkalescent that pure water, which is modulated, pH value 7.5-8.5, and pure according to mass percent
Peptone 0.3%, sodium citrate 0.3%, yeast extract 0.2%, sodium acid carbonate 0.2%, sodium chloride 0.2%, chlorination are added in water
Ammonium 0.2%, epsom salt 0.2%, potassium dihydrogen phosphate 0.2%, surplus are pure water；
B. actication of culture：Using sealing container as incubator, culture medium obtained by step A is filled to the 70% of volume,
And photosynthetic bacteria strain is inoculated in culture medium, 27-32 DEG C of cultivation temperature, incubation time 100-120h, obtain activated spawn；
C. seed liquor culture：Activated spawn obtained by above-mentioned step B is subjected to fermented and cultured, the temperature of culture is 27-32
DEG C, illumination condition is 2500Lx~3000Lx；Fermentation medium need to be added, the fermentation medium adds in every 100ml pure water
Enter potassium propionate 0.2g, ammonium chloride 0.1g, yeast extract 0.2g, vitamin 0.1g.
The made photosynthetic microbial inoculum of the present invention is more efficient, better quality.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize invention
The equivalent structure or equivalent flow conversion that description is made, or other related technical areas are directly or indirectly used in,
It is included within the scope of the present invention.
Claims (2)
1. a kind of photosynthetic bacterial preparation process, it is characterised in that comprise the following steps：
A. prepared by culture medium：It is in alkalescent that pure water, which is modulated, pH value 7.5-8.5, and according in mass percent pure water
Add peptone 0.3%, sodium citrate 0.3%, yeast extract 0.2%, sodium acid carbonate 0.2%, sodium chloride 0.2%, ammonium chloride
0.2%, epsom salt 0.2%, potassium dihydrogen phosphate 0.2%, surplus is pure water；
B. actication of culture：Using sealing container as incubator, the culture medium obtained by step A is filled to the 70% of volume, and incite somebody to action
Photosynthetic bacteria strain is inoculated in culture medium, 27-32 DEG C of cultivation temperature, incubation time 100-120h, obtains activated spawn；
C. seed liquor culture：Activated spawn obtained by above-mentioned step B is subjected to fermented and cultured, the temperature of culture is 27-32 DEG C, light
It is 2500Lx~3000Lx according to condition.
2. photosynthetic bacterial preparation process according to claim 1, it is characterised in that in the step C, fermentation training need to be added
Base is supported, the fermentation medium adds potassium propionate 0.2g, ammonium chloride 0.1g, yeast extract 0.2g, vitamin in every 100ml pure water
0.1g。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711136123.8A CN107653211A (en) | 2017-11-16 | 2017-11-16 | A kind of photosynthetic bacterial preparation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711136123.8A CN107653211A (en) | 2017-11-16 | 2017-11-16 | A kind of photosynthetic bacterial preparation process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107653211A true CN107653211A (en) | 2018-02-02 |
Family
ID=61120471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711136123.8A Withdrawn CN107653211A (en) | 2017-11-16 | 2017-11-16 | A kind of photosynthetic bacterial preparation process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107653211A (en) |
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2017
- 2017-11-16 CN CN201711136123.8A patent/CN107653211A/en not_active Withdrawn
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Legal Events
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180202 |