CN107646654A - STEVIA REBAUDIANA cuttage and seedling culture matrix and preparation method thereof - Google Patents

STEVIA REBAUDIANA cuttage and seedling culture matrix and preparation method thereof Download PDF

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Publication number
CN107646654A
CN107646654A CN201711081455.0A CN201711081455A CN107646654A CN 107646654 A CN107646654 A CN 107646654A CN 201711081455 A CN201711081455 A CN 201711081455A CN 107646654 A CN107646654 A CN 107646654A
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parts
cuttage
stevia rebaudiana
seedling culture
fermentation
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Inventor
王冬良
王瑞生
李媛媛
岳凡凡
丁银媛
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Anhui Agricultural University AHAU
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Anhui Agricultural University AHAU
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Abstract

The present invention provides a kind of STEVIA REBAUDIANA cuttage and seedling culture matrix and preparation method thereof, and in terms of weight fraction, seedling medium includes following composition:Ferment 25 40 parts of organic fertilizer, 15 30 parts of perlite, 5 15 parts of clinker, 0.02 0.1 parts of fertilizer, 5 10 parts of water-loss reducer.The STEVIA REBAUDIANA cuttage and seedling culture substrate seedling cycle is fast, and survival rate is high.

Description

STEVIA REBAUDIANA cuttage and seedling culture matrix and preparation method thereof
Technical field
The invention belongs to plant cuttage propagation technical field, and in particular to a kind of STEVIA REBAUDIANA cuttage and seedling culture matrix and its preparation Method.
Background technology
Matrix is material the most key in nursery, be determine STEVIA REBAUDIANA root system growing environment an important factor for, it except Outside fixed plant, moreover it is possible to provide nutrient and moisture for the production of plant;Different matrix have a great influence to nursery, matrix quality Quality directly affects the success of seedling-raising technique;The application of modern solid matrix is relatively broad, is made and educated using stalk fermentation Seedling matrix is also relatively universal, but matrix raw material are single mostly, and physical behavior and chemical property are all undesirable after fermentation;STEVIA REBAUDIANA skewer It is big to be inserted through journey water requirement, it is especially also larger in later stage fertilizer requirement, it is general to carry out cuttage and seedling culture with husky or garden mould, matrix be present and do not protect Water, it is airtight, the problems such as later stage fertilizer scarcity.
The content of the invention
An object of the present invention is to provide a kind of STEVIA REBAUDIANA cuttage and seedling culture matrix, and the cuttage and seedling culture matrix can be carried effectively High-survival rate, improve nursery speed.
The second object of the present invention is the preparation method for providing above-mentioned STEVIA REBAUDIANA cuttage and seedling culture matrix.
STEVIA REBAUDIANA cuttage and seedling culture matrix of the present invention, in terms of weight fraction, including following composition:Fermentation organic fertilizer 25-40 parts, Perlite 15-30 parts, clinker 5-15 parts, fertilizer 0.02-0.1 parts, water-loss reducer 5-10 parts.
Preferably, wherein described fermentation organic fertilizer is one or both of fermentation poultry dung or fermented stalk;It is preferred that Fermentation poultry dung and fermented stalk compounding use, compound proportion are (1-2): 1.
The fine sand of 5-7 parts can be also mixed into matrix.
The preparation method of wherein described fermentation poultry dung is as follows:
(1) by one kind in 70-80 parts dried poultrymanure, 4-7 parts leavening, 10-20 parts rice bran, corn, wheat bran or soya-bean cake or It is several, 4-6 part alcohol is added, is stirred, obtains chicken manure matrix;
(2) chicken manure matrix pH value is adjusted to 6-8;
(3) chicken manure water content of substrate is adjusted, moisture content is maintained between 60~65%;
(4) chicken manure builds heap, and stack height is in 1-2m, and width is in 1.5-3m, and length is in 2-4m;
(5) ventilation is mixed thoroughly:Chicken manure heap is made into bulk shape, bamboo pole with holes is inserted every 50-80cm, ensures ventilation, and Timing is stirred, to carry out aerobic fermentation.
For chicken manure after accumulation 2 days, temperature can be increased to 50~60 DEG C, can reach more than 65 DEG C within the 3rd day, in this temperature Under should stir once, generally, be likely to occur 2~3 more than 65 DEG C of high temperature during the fermentation, so, 2 need to be stirred Fermentation process can be completed for~3 times, the next week left and right of normal condition can ferment completion.
5-10 part edible mushroom rod discarded objects can also be added in step (1) to ferment together, described edible mushroom rod discarded object It is 100-300 mesh that particle diameter is crushed to during use, the preferred pleurotus eryngii bacteria stick discarded object of bacteria stick, and its original pleurotus eryngii is (raw with culture medium Culture medium before production pleurotus eryngii) it is prepared from the following raw materials in parts by weight:Mulberry tree branch powder 20-25 parts, cotton seed hulls 10-20 parts, wheat Bran 20-25 parts, dregs of beans 5-10 parts, corncob 5-10 parts, corn flour 5-10 parts, precipitated calcium carbonate 0.5-1.5 parts.
The preparation method of wherein described fermented stalk is as follows:
Prepare Step 1: making fertile material:It is maize straw or wheat stalk to make fertile material:Such as using maize straw need through Coarse crushing is crossed, wheat stalk then can be used directly;
Step 2: leavening is activated, leavening Activiation method:4-7 part brown sugar is added in 10-15 part kg of water, is boiled After opening, 10-20 part water is added in the brown sugar water boiled, cools down, finally leavening 5-10 parts is poured into water again and stirred evenly, is filled After bucket is sealed by fermentation 6-8 days, leavening is activated by completing when smelling tart flavour or vinosity, and above number is parts by weight;
Step 3: pile-fermentation:Leavening after 25-35 parts are activated is added in the fertilizer material processed of 900-1000 parts, together Shi Jiashui, add the principle of water, added from less to more when adding water, the moisture content of fermentation material is maintained at 55- 65%;Then heap is built, stack height is in 1-2m, and width 1.5-3m, length is in 2-4m;
Step 4: stalk degenerates process.
It can substantially divide with the next stage from the change of stalk heap temperature:1. the temperature rise period.I.e. stalk heap temperature is raised to 50 DEG C from normal temperature It is the left and right stage, general only to need 1 day or so;2. hot stage.70 DEG C of stages are raised to from 50 DEG C, it is general only to need 2 days;3. cool rank Section;That is continuous high temperature starts to cool after 1-2 weeks;Now the process of stalk fermentation system fertilizer is completed.
Wherein described fertilizer is urea, sodium dihydrogen phosphate and potassium carbonate compounding, and compounding weight ratio is (2-3): (3-5): 1。
Wherein described water-loss reducer is polyacrylamide (PAM).
The particle diameter of clinker and perlite is 100-300 mesh in STEVIA REBAUDIANA cuttage and seedling culture matrix of the present invention.
The preparation method of STEVIA REBAUDIANA cuttage and seedling culture matrix of the present invention is as follows:
Weigh up batch raw material quantity:Raw material mixing is weighed up in proportion, and water content 14%-19% is adjusted during use.
STEVIA REBAUDIANA cuttage and seedling culture matrix of the present invention has the beneficial effect that:
(1) present invention is by the nutrient demand and physiological status during studying STEVIA REBAUDIANA cuttage, for the growth phase STEVIA REBAUDIANA made a kind of cultivation matrix to measure, using the matrix carry out STEVIA REBAUDIANA cuttage and seedling culture, by cuttings insert matrix after, It is viable without fertilising, and can cultivates into strong sprout, it is simple and easy;
(2) matrix disclosed by the invention, survival rate can be effectively improved:The cuttage survival rate one of general common solid matrix As 85%~90%, and use this matrix to carry out the cuttage and seedling culture of STEVIA REBAUDIANA, survival rate reaches as high as 100%;
(3) matrix disclosed by the invention, cultivation speed can be effectively improved:With general mechanism carry out cuttage, STEVIA REBAUDIANA from Cuttage seeding, which grows into strong sprout, needs the time in or so three week, and uses the cuttage and seedling culture of this matrix progress STEVIA REBAUDIANA, cuttage seeding Through the times of two weeks or so can rooting and transplant, cultivate speed;
(4) matrix uses the agricultural wastes such as stalk, chicken manure, by its recycling, and utilizes perlite, clinker Deng can accomplish to place, free from extraneous odour, green harmless to people and animals for a long time;
(5) matrix is by adding clinker, urea, NaH2PO4、K2CO3, soil pH value is adjusted to neutrality, is stablized it and is contained Some nutrients, obtain more preferable chemical stability;By adding a certain proportion of perlite, adjust soil porosity and Water content, so as to obtain physically better stability.
(6) the preparation method preparation amount of the matrix is larger, step clear and definite, meets matrix and prepares on a large scale, STEVIA REBAUDIANA The requirement of extensive cuttage and seedling culture.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with example, but those skilled in the art will manage Solution, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.
Water-loss reducer is polyacrylamide (PAM) in embodiment.
Fermentation poultry dung A preparation method is as follows in embodiment:
(1) by 75 kilograms of dried poultrymanures, 5 kilograms of " Nong Shengle " board leavenings, 15 kilograms of soya-bean cakes, 5 kilograms of alcohol is added, are stirred Mix uniformly, obtain chicken manure matrix;
(2) chicken manure matrix pH value is adjusted to 6-8;
(3) chicken manure water content of substrate is adjusted, moisture content is maintained between 60~65%;
(4) chicken manure builds heap, and stack height is in 1.5m, and width 2m, length is in 3m;
(5) ventilation is mixed thoroughly:Chicken manure heap is made into bulk shape, inserts a bamboo pole every 50cm in width, bamboo pole is straight Footpath 3-5cm, bamboo pole will punch, and hole knockout is that every bamboo pole uniformly makes a call to 4 row holes vertically, and line space is identical, the pitch of holes of each row For 7cm, aperture 2-3cm, ensure ventilation, and timing overturns, to carry out aerobic fermentation.
For chicken manure after accumulation 2 days, temperature is increased to 50~60 DEG C, can reach more than 65 DEG C within the 3rd day, turn at this temperature 1 time, 3 more than 65 DEG C of high temperature are likely to occur during the fermentation, so, need to overturn can complete fermentation process for 3 times, and 7 Its fermentation is completed.
Fermentation poultry dung B preparation method is as follows in embodiment:
(1) 75 kilograms of dried poultrymanures, 5 kilograms of " Nong Shengle " board leavenings, 15 kilograms of soya-bean cakes, 5 kilograms of edible mushroom rods are discarded Thing, 5 parts of alcohol are added, are stirred, obtain chicken manure matrix;
(2) chicken manure matrix pH value is adjusted to 6-8;
(3) chicken manure water content of substrate is adjusted, moisture content is maintained between 60~65%;
(4) chicken manure builds heap, and stack height is in 1.5m, and width 2m, length is in 3m;
(5) mix ventilation thoroughly, chicken manure heap is made into bulk shape, insert a bamboo pole every 50cm in width, bamboo pole is straight Footpath 3-5cm, bamboo pole will punch, and hole knockout is that every bamboo pole uniformly makes a call to 4 row holes vertically, and line space is identical, the pitch of holes of each row For 7cm, aperture 2-3cm, ensure ventilation, and timing is stirred, to carry out aerobic fermentation.
For chicken manure after accumulation 2 days, temperature is increased to 50~60 DEG C, reaches more than 65 DEG C within the 3rd day, overturns 1 at this temperature It is secondary, occur 3 more than 65 DEG C of high temperature during the fermentation, so, need to overturn 3 times can complete fermentation process, ferment within 7 days Into.
Described edible mushroom rod discarded object using when to be crushed to particle diameter be 100-300 mesh, bacteria stick is pleurotus eryngii bacteria stick, and it is former Beginning pleurotus eryngii is prepared from the following raw materials in parts by weight with culture medium (culture medium before production pleurotus eryngii):35 parts of mulberry tree branch powder, 15 parts of cotton seed hulls, 25 parts of wheat bran, 10 parts of dregs of beans, 10 parts of corncob, 7 parts of corn flour, 1 part of precipitated calcium carbonate.
The preparation method of fermented stalk described in embodiment is as follows:
Prepare Step 1: making fertile material:It is wheat stalk to make fertile material;
Step 2: leavening is activated, leavening is " agriculture Citroen zx " organic leavening, Activiation method:In 15 parts of kg of water 5 kilograms of brown sugar of middle addition, after boiling, add 10 kg of water in the brown sugar water boiled, and cool down, finally again that leavening 5 is public Jin, which is poured into water, to be stirred evenly, and after barrelling is sealed by fermentation 7 days, leavening is activated by completing when smelling tart flavour or vinosity;
Step 3: pile-fermentation:Leavening after 25-35 kilograms of activation is added to 900-1000 kilograms of fertilizer material processed In, while add water, add the principle of water, added from less to more when adding water, the moisture content of fermentation material is maintained at 55-65%;Then heap is built, stack height is in 1-2m, and width 1.5-3m, length is in 2-4m;
Step 4:Stalk degenerates process;It can substantially divide with the next stage from the change of stalk heap temperature:1. the temperature rise period.That is stalk Heap temperature is raised to 50 DEG C or so stages from normal temperature, 1 day;2. hot stage.70 DEG C of stages are raised to from 50 DEG C, are only needed 2 days;3. drop Thermophase.I.e. continuous high temperature starts to cool after 10 days;Now the process of stalk fermentation system fertilizer is completed.
Fertilizer used is urea in embodiment:NaH2PO4∶K2CO3=2: 4: 1.
Embodiment 1-6 formula is shown in Table 1;Embodiment 7-9 and comparative example 1-3 formula are shown in Table 2.
Table 1
Embodiment 1 2 3 4 5 6
Fermentation poultry dung A 30 - - 15 - -
Fermentation poultry dung B - 30 - - 15 20
Fermented stalk - - 30 15 15 10
Perlite 20 20 20 20 20 15
Clinker 10 10 10 10 10 15
Fertilizer 0.02 0.02 0.02 0.02 0.02 0.03
Water-loss reducer 5 5 5 5 5 6
Table 2
Embodiment 7 8 9 Comparative example 1 Comparative example 2 Comparative example 3
Fermentation poultry dung A - - - - - -
Fermentation poultry dung B 16 20 17 37 5 17
Fermented stalk 14 20 13 13 13 13
Perlite 19 17 20 20 20 10
Clinker 11 13 10 10 10 20
Fertilizer 0.03 0.02 0.02 0.02 0.02 0.02
Water-loss reducer 5 5 5 5 5 5
Fine sand - - 5 5 5 5
Comparative example 1 increases the dosage of fermentation poultry dung on the basis of embodiment 9, and other are same as Example 9, comparative example 2 reduce the dosage of fermentation poultry dung on the basis of embodiment 9, and other are same as Example 9, and comparative example 3 is by perlite and stove The dosage exchange of slag, other are same as Example 9.
STEVIA REBAUDIANA cuttage is carried out using the seedling medium of above example and comparative example, containing for seedling medium is adjusted during use Water is 16%-19%, and cottage method is as follows:
1st, cuttings is handled:Selection is healthy and strong, the annual branch of no disease and pests harm does cuttings, each cutting length 5cm or so, strain Line-spacing is 2.5cm × 5cm;Matrix and cutting are carried out disinfection with 800-1000 times of liquid of Bravo before inserting;
2nd, 20-28 DEG C of keeping temperature after cuttage, humidity more than 85%, can take root within two weeks or so, and radical is more, can enter Row transplanting.
Above example, the effect of comparative example are shown in Table 3.
Table 3
Test strain number Survive strain number Take root required time/day Strain number is survived after transplanting
Embodiment 1 100 90 16 85
Embodiment 2 100 91 15 86
Embodiment 3 100 92 15 87
Embodiment 4 100 92 15 90
Embodiment 5 100 99 13 99
Embodiment 6 100 99 13 99
Embodiment 7 100 99 13 99
Embodiment 8 100 99 13 99
Embodiment 9 100 100 14 100
Comparative example 1 100 65 14 50
Comparative example 2 100 86 20 83
Comparative example 3 100 90 18 90
As can be seen from the above embodiments, STEVIA REBAUDIANA is coordinated with fermented stalk added with the fermentation poultry dung of edible mushroom rod discarded object The effect that cuttage obtains is best, has synergy with fermented stalk added with the fermentation poultry dung of edible mushroom rod discarded object
Above content describes the general principle and principal character of the present invention, and the present invention is not limited to the above embodiments, Without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, and these changes and improvements are all Fall into scope of the claimed invention.

Claims (10)

1. a kind of STEVIA REBAUDIANA cuttage and seedling culture matrix, in terms of weight fraction, including following composition:Fermentation organic fertilizer 25-40 parts, pearl Rock 15-30 parts, clinker 5-15 parts, fertilizer 0.02-0.1 parts, water-loss reducer 5-10 parts.
2. STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 1, it is characterised in that described fermentation organic fertilizer is fermentation One or both of chicken manure or fermented stalk.
3. STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 1, it is characterised in that fermentation organic fertilizer be fermentation poultry dung and Fermented stalk compounding use, compound proportion 1-2: 1.
4. STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 1, it is characterised in that can also be mixed into the fine sand of 5-7 parts.
5. STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 2, it is characterised in that the preparation method of the fermentation poultry dung It is as follows:
(1) by the one or more in 70-80 parts dried poultrymanure, 4-7 parts leavening, 10-20 parts rice bran, corn, wheat bran or soya-bean cake, 4-6 part alcohol is added, is stirred, obtains chicken manure matrix;
(2) chicken manure matrix pH value is adjusted to 6-8;
(3) chicken manure water content of substrate is adjusted, moisture content is maintained between 60~65%;
(4) chicken manure builds heap, and stack height is in 1-2m, and width is in 1.5-3m, and length is in 2-4m;
(5) ventilation is mixed thoroughly:Bamboo pole with holes is inserted every 50-80cm, and timing is stirred, to carry out aerobic fermentation.
6. STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 5, it is characterised in that step can also add 5-10 in (1) Part edible mushroom rod discarded object ferments together, described edible mushroom rod discarded object using when be crushed to particle diameter 100-300 mesh.
7. STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 6, it is characterised in that the edible mushroom rod discarded object is apricot Abalone mushroom bacteria stick discarded object, its original pleurotus eryngii are prepared from the following raw materials in parts by weight with culture medium:Mulberry tree branch powder 20-25 parts, Cotton seed hulls 10-20 parts, wheat bran 20-25 parts, dregs of beans 5-10 parts, corncob 5-10 parts, corn flour 5-10 parts, precipitated calcium carbonate 0.5- 1.5 part.
8. STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 2, it is characterised in that the preparation method of the fermented stalk It is as follows:
Prepare Step 1: making fertile material:It is maize straw or wheat stalk to make fertile material:It need to such as be passed through using maize straw thick Crush, wheat stalk then can be used directly;
Step 2: leavening is activated, leavening Activiation method:4-7 part brown sugar is added in 10-15 part kg of water, after boiling, 10-20 part water is added in the brown sugar water boiled, cools down, finally leavening 5-10 parts is poured into water again and stirred evenly, barrelling is close Seal ferment is after 6-8 days, and leavening activation completion when smelling tart flavour or vinosity, above number is parts by weight;
Step 3: pile-fermentation:Leavening after 25-35 parts are activated is added in the fertilizer material processed of 900-1000 parts, is added simultaneously Water, the moisture content of fermentation material is maintained at 55-65%;Then build heap, stack height in 1-2m, width 1.5-3m, Length is in 2-4m;
Step 4: stalk is degenerated.
9. STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 1, it is characterised in that described fertilizer is urea, di(2-ethylhexyl)phosphate Hydrogen sodium and potassium carbonate compounding, compounding weight ratio is (2-3): (3-5): 1.
10. the preparation method of STEVIA REBAUDIANA cuttage and seedling culture matrix according to claim 1, it is characterised in that include following step successively Suddenly:
Weigh up batch raw material quantity:Raw material mixing, regulation water content 14%-19% are weighed up in proportion.
CN201711081455.0A 2017-11-06 2017-11-06 STEVIA REBAUDIANA cuttage and seedling culture matrix and preparation method thereof Pending CN107646654A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108849386A (en) * 2018-05-29 2018-11-23 安徽润航农业科技开发有限公司 A kind of low cost soilless culture substrate
CN109197550A (en) * 2018-11-14 2019-01-15 湖南省银鑫油茶开发有限公司 A kind of tea oil tree propagation method
CN109197200A (en) * 2018-11-07 2019-01-15 常宁市瑶园生态农业科技发展有限公司 Tea cutting mating system
CN112703990A (en) * 2020-12-23 2021-04-27 长治学院 Matrix suitable for forest cutting seedling transplanting and preparation method and application thereof

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CN102090306A (en) * 2010-11-25 2011-06-15 海城市三星生态农业有限公司 Stevia rebaudiana seedling medium and preparation method thereof
CN106171907A (en) * 2016-08-18 2016-12-07 张玉玲 A kind of two sections of floating plate cuttage seedling raising methods of Folium Stevlae Rebaudianae
CN106748449A (en) * 2016-12-11 2017-05-31 衡阳县轩健农牧发展有限公司 A kind of special fertilizer for cole with fermentation poultry dung as primary raw material and preparation method thereof
CN107079732A (en) * 2017-04-05 2017-08-22 周兴虎 A kind of willow cuttage and seedling culture matrix

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101715714A (en) * 2009-11-02 2010-06-02 东北师范大学 Moisture-preserving nutritious soil for growing herbaceous flower seedlings and preparation method thereof
CN102090306A (en) * 2010-11-25 2011-06-15 海城市三星生态农业有限公司 Stevia rebaudiana seedling medium and preparation method thereof
CN106171907A (en) * 2016-08-18 2016-12-07 张玉玲 A kind of two sections of floating plate cuttage seedling raising methods of Folium Stevlae Rebaudianae
CN106748449A (en) * 2016-12-11 2017-05-31 衡阳县轩健农牧发展有限公司 A kind of special fertilizer for cole with fermentation poultry dung as primary raw material and preparation method thereof
CN107079732A (en) * 2017-04-05 2017-08-22 周兴虎 A kind of willow cuttage and seedling culture matrix

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108849386A (en) * 2018-05-29 2018-11-23 安徽润航农业科技开发有限公司 A kind of low cost soilless culture substrate
CN109197200A (en) * 2018-11-07 2019-01-15 常宁市瑶园生态农业科技发展有限公司 Tea cutting mating system
CN109197550A (en) * 2018-11-14 2019-01-15 湖南省银鑫油茶开发有限公司 A kind of tea oil tree propagation method
CN112703990A (en) * 2020-12-23 2021-04-27 长治学院 Matrix suitable for forest cutting seedling transplanting and preparation method and application thereof

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Application publication date: 20180202