CN107638375A - A kind of the spot removal and skin care liquid and preparation method of the extract of stem cell containing blueberry - Google Patents
A kind of the spot removal and skin care liquid and preparation method of the extract of stem cell containing blueberry Download PDFInfo
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- CN107638375A CN107638375A CN201711055966.5A CN201711055966A CN107638375A CN 107638375 A CN107638375 A CN 107638375A CN 201711055966 A CN201711055966 A CN 201711055966A CN 107638375 A CN107638375 A CN 107638375A
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Abstract
The invention discloses a kind of the spot removal and skin care liquid and preparation method of the extract of stem cell containing blueberry, belong to technical field of skin care, the composition of nti-freckle includes the component of following mass fraction：4~7 parts of 9~16 parts of blueberry stem cell extract, 6~12 parts of pot marigold extract and camellia flower extract；The weight/mass percentage composition of composition is 13.7% in shin moisturizer.Not but not damage skin, and spot-removal function is not good for the composition of the present invention and the shin moisturizer for preparing.
Description
Technical field
The invention belongs to technical field of skin care, more particularly to a kind of spot removal and skin care liquid of stem cell containing blueberry extract and
Preparation method.
Background technology
With the improvement of living standards, people increasingly pay attention to the aesthetic feeling of appearance, therefore skin care item industry is vigorously sent out
Exhibition.Existing skin care item species is various, there is sunlight screening skin-protecting product, Moisturizer and spot-eliminating skin care product etc..However, existing nti-freckle
The synthesis chemical reagent added in skin care item is excessive, easily damages skin；Secondly, it is poor the effect of nti-freckle.
Therefore, it is necessary to prepare new composition and skin care item, its not but not damage skin, and the work(of nti-freckle
Imitate.
The content of the invention
Present invention aims to overcome that the shortcomings of the prior art, and a kind of dispelling for extract of stem cell containing blueberry is provided
The shin moisturizer of spot shin moisturizer and preparation method, said composition and its preparation is not but not damage skin, and good the effect of nti-freckle.
To achieve the above object, the technical scheme taken of the present invention is：A kind of composition of nti-freckle, it includes following quality
The component of number：4~7 parts of 9~16 parts of blueberry stem cell extract, 6~12 parts of pot marigold extract and camellia flower extract.
Blueberry stem cell extract contains various active composition, such as anthocyanidin, and anthocyanidin has antioxidation activity, can reduce
The degree of injury that face's epidermal stem cells induce under extraneous factor, epidermal stem cells are made to maintain vigour, continuous updating.Pot marigold
Extract is rich in multivitamin and lutein, has oxidation resistant effect, can prevent pigementation, promote skin gloss with
Elasticity.Camellia flower extract can remove color spot and sliding tender skin；Camellia contains various active composition, such as Tea Polyphenols, and tea is more
Phenol can remove free radical.
The preparation method of blueberry stem cell extract comprises the following steps：
1) part for blueberry pulp is chosen, carry out disinfection processing；Chopping is seeded on MS culture mediums, dark training at 25 DEG C
Support；The outer paraphyte of propagation is taken out after two weeks, separates and is transferred in subculture medium and cultivate, subculture once, obtains every two weeks
To blueberry stem cell；
2) culture medium after culture blueberry stem cell is collected, through ultrafiltration membrance filter, concentration filtrate, retains the work in culture medium
Property composition；Culture medium and the lysate of blueberry stem cell are freeze-dried, obtain blueberry stem cell extract.
The composition of the present invention includes blueberry stem cell extract, pot marigold extract and camellia flower extract, blueberry and done
Cell extract, pot marigold extract and camellia flower extract can keep face's epidermal stem cells in the range of certain proportion
Vigor, continuous updating, can efficiently remove face's color spot；And said composition is mostly natural materials, to skin zero damage.
As the improvement of above-mentioned technical proposal, the mass ratio of the blueberry stem cell extract and the pot marigold extract
For 0.9~1.5.When the mass ratio of blueberry stem cell extract and pot marigold extract is 0.9~1.5, blueberry stem cell carries
Take thing that there is the effect of cooperateing with nti-freckle with pot marigold extract.
As the further improvement of above-mentioned technical proposal, the blueberry stem cell extract and the pot marigold extract
Mass ratio is 1.When the mass ratio of blueberry stem cell extract and pot marigold extract is 1, blueberry stem cell extract and gold
The effect of small cup flower extract collaboration nti-freckle, is more notable.
As the further improvement of above-mentioned technical proposal, composition is made up of the component of following mass fraction：Blueberry is done
5.4 parts of 10 parts of cell extract, 10 parts of pot marigold extract and camellia flower extract.
In addition, the present invention also provides application of the described composition in skin care item.
In addition, the present invention also provides a kind of shin moisturizer for including described composition.
As the improvement of above-mentioned technical proposal, the weight/mass percentage composition of composition is 13.7% in the shin moisturizer.
As the improvement of above-mentioned technical proposal, the shin moisturizer is made up of the component of following weight/mass percentage composition：Composition
9%th, NMF 20% and emulsifying agent 17%, surplus are deionized water.
In addition, the present invention also provides a kind of preparation method of described shin moisturizer, comprise the following steps successively：
S1) composition is added in deionized water, in 76 DEG C of heating stirring 1.6h, obtains mixture I；
S2) to step S1) NMF and emulsifying agent are added in the mixture I, in 62 DEG C of heating stirring 1.5h, after cooling
Obtain shin moisturizer.
The beneficial effects of the present invention are：The present invention provides a kind of the spot removal and skin care liquid and system of the extract of stem cell containing blueberry
Preparation Method, blueberry stem cell extract in composition and shin moisturizer of the invention, pot marigold extract and camellia flower extract
Quality can efficiently remove face's color spot in the range of certain proportion；And in said composition and shin moisturizer be mostly natural materials,
To skin zero damage；In addition, blueberry stem cell extract and pot marigold extract can cooperate with nti-freckle.
Embodiment
For the object, technical solutions and advantages of the present invention are better described, below in conjunction with specific embodiment and subordinate list pair
The present invention is described further.
Embodiment 1
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：9 parts of blueberry stem cell extract, golden small cup
6 parts of 11 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The present embodiment also provides the preparation method of the shin moisturizer, comprises the following steps successively：
S1) composition is added in deionized water, in 76 DEG C of heating stirring 1.6h, obtains mixture I；
S2) to step S1) NMF and emulsifying agent are added in the mixture I, in 62 DEG C of heating stirring 1.5h, after cooling
Obtain shin moisturizer.
The preparation method of blueberry stem cell extract comprises the following steps in composition：
1) part for blueberry pulp is chosen, carry out disinfection processing；Chopping is seeded on MS culture mediums, dark training at 25 DEG C
Support；The outer paraphyte of propagation is taken out after two weeks, separates and is transferred in subculture medium and cultivate, subculture once, obtains every two weeks
To blueberry stem cell；
2) culture medium after culture blueberry stem cell is collected, through ultrafiltration membrance filter, concentration filtrate, retains the work in culture medium
Property composition；Culture medium and the lysate of blueberry stem cell are freeze-dried, obtain blueberry stem cell extract.
Embodiment 2
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：16 parts of blueberry stem cell extract, golden small cup
4 parts of 6 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Embodiment 3
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：10 parts of blueberry stem cell extract, golden small cup
7 parts of 12 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Embodiment 4
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：12 parts of blueberry stem cell extract, golden small cup
6 parts of 6 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Embodiment 5
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：11.2 parts of blueberry stem cell extract, gold
5.3 parts of 7 parts of small cup flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Embodiment 6
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：9 parts of blueberry stem cell extract, golden small cup
5.3 parts of 10 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Embodiment 7
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：10 parts of blueberry stem cell extract, golden small cup
5.4 parts of 10 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Embodiment 8
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：12 parts of blueberry stem cell extract, golden small cup
6 parts of 8 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Comparative example 1
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：9 parts of blueberry stem cell extract, golden small cup
4 parts of 13 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Comparative example 2
A kind of composition of nti-freckle, it is made up of the component of following mass fraction：16 parts of blueberry stem cell extract, golden small cup
7 parts of 5 parts of flower extract and camellia flower extract.
The present embodiment also provides a kind of shin moisturizer, and it is made up of the component of following weight/mass percentage composition：Composition 13.7%,
NMF (glycerine) 10.4% and emulsifying agent (sorbierite olive grease) 16.9%, surplus is deionized water.
The preparation method of shin moisturizer described in the present embodiment refers to embodiment 1.
Effect example 1
The structure of color spot guinea pig model：It is experimental subjects to choose Healthy female brown color cavy, wipes out guinea pig back area
For 10cm × 10cm hair, using UVB ultra violet lamps, the spectral value of ultraviolet lamp tube is 280~320nm, and fluorescent tube is apart from globefish
The back distance of mouse is 15cm, and irradiation total amount is 500mJ/cm, Continuous irradiation 10 weeks, counts guinea pig back skin spots
Number.
150 color spot cavys are chosen, are randomly divided into 5 groups, every group 30；The skin of back of every group of cavy is early, evening each smearing 1
Secondary shin moisturizer, it is carried out continuously 4 weeks, statistics smears the spot number of guinea pig back skin appearance after shin moisturizer, calculates spot clearance rate.
Spot clearance rate=(smearing the spot number after spot number-smearing shin moisturizer before shin moisturizer)/smears the spot number before skin care item
× 100%.
A1 groups use the shin moisturizer of embodiment 1, and B1 groups use the shin moisturizer of embodiment 2, and C1 groups use the skin care of embodiment 3
Liquid, D1 groups use the shin moisturizer of comparative example 1, and for E1 groups using the shin moisturizer of comparative example 2, measurement result is as shown in table 1.
Table 1
| Group | A1 groups | B1 groups | C1 groups | D1 groups | E1 groups |
| Spot clearance rate/% | 17.3 | 16.9 | 16.4 | 8.2 | 7.0 |
As shown in table 1, the spot clearance rate of A1, B1 and C1 group is more than the spot clearance rate of D1 and E1 groups；As can be seen here, when
The mass fraction of blueberry stem cell extract is 9~16 parts in composition, and the mass fraction of pot marigold extract is 6~12 parts,
When the mass fraction of camellia flower extract is 4~7 parts, the spot-removal function of composition and shin moisturizer is preferable.
Effect example 2
With reference to effect example 1, cavy is randomly divided into 5 groups：A2 groups use the shin moisturizer of embodiment 1, and B2 groups use embodiment 2
Shin moisturizer, C2 groups use the shin moisturizer of embodiment 3, and D2 groups use the shin moisturizer of embodiment 4, and E2 groups use the skin care of embodiment 5
Liquid, measurement result are as shown in table 2.
Table 2
| Group | A2 groups | B2 groups | C2 groups | D2 groups | E2 groups |
| Spot clearance rate/% | 17.3 | 16.9 | 16.4 | 22.1 | 23.0 |
As shown in table 2, the spot clearance rate of A2, B2 and C2 group is less than the spot clearance rate of D2 and E2 groups；As can be seen here, when
The mass fraction of blueberry stem cell extract is 9~12 parts in composition, and the mass fraction of pot marigold extract is 6~10 parts,
When the mass fraction of camellia flower extract is 5~6 parts, the spot-removal function of composition and shin moisturizer is good.
Effect example 3
With reference to effect example 1, cavy is randomly divided into 5 groups：A3 groups use the shin moisturizer of embodiment 4, and B3 groups use embodiment 5
Shin moisturizer, C3 groups use the shin moisturizer of embodiment 6, and D3 groups use the shin moisturizer of embodiment 7, and E3 groups use the skin care of embodiment 8
Liquid, measurement result are as shown in table 3.
Table 3
| Group | A3 groups | B3 groups | C3 groups | D3 groups | E3 groups |
| Spot clearance rate/% | 22.1 | 23.0 | 26.9 | 31.1 | 27.1 |
As shown in table 3, the spot clearance rate of A3 and B3 groups is less than the spot clearance rate of C3 and E3 groups, the spot of C3 and E31 groups
Point clearance rate is less than the spot clearance rate of D3 groups；As can be seen here, when the mass fraction of blueberry stem cell extract in composition is 9
~12 parts, the mass fraction of pot marigold extract is 6~10 parts, and the mass fraction of camellia flower extract is 5~6 parts, and blueberry
When the mass ratio of stem cell extract and the pot marigold extract is 0.9~1.5, the spot-removal function of composition and shin moisturizer is more
It is good；Shin moisturizer especially in the embodiment of the present application 7 (mass ratio of blueberry stem cell extract and the pot marigold extract is 1)
Spot-removal function is more preferable.
Finally, it should be noted that above example to illustrate technical scheme rather than to the present invention protect
The limitation of scope, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should manage
Solution, technical scheme can be modified or replaced on an equal basis, without departing from technical solution of the present invention essence and
Scope.
Claims (10)
1. a kind of composition of nti-freckle, it is characterised in that include the component of following mass fraction：Blueberry stem cell extract 9~
4~7 parts of 16 parts, 6~12 parts of pot marigold extract and camellia flower extract.
2. composition as claimed in claim 1, it is characterised in that include the component of following mass fraction：Blueberry stem cell carries
Take 5~6 parts of 9~12 parts of thing, 6~10 parts of pot marigold extract and camellia flower extract.
3. composition as claimed in claim 1 or 2, it is characterised in that the blueberry stem cell extract and the pot marigold
The mass ratio of extract is 0.9~1.5.
4. composition as claimed in claim 3, it is characterised in that the blueberry stem cell extract extracts with the pot marigold
The mass ratio of thing is 1.
5. composition as claimed in claim 4, it is characterised in that be made up of the component of following mass fraction：Blueberry stem cell
5.4 parts of 10 parts of extract, 10 parts of pot marigold extract and camellia flower extract.
6. application of the composition as described in any one of Claims 1 to 5 in skin care item.
A kind of 7. shin moisturizer for including the composition as described in power requires 1~5 any one.
8. shin moisturizer as claimed in claim 7, it is characterised in that the weight/mass percentage composition of composition is in shin moisturizer
13.7%.
9. shin moisturizer as claimed in claim 7, it is characterised in that be made up of the component of following weight/mass percentage composition：Composition
13.7%th, NMF 10.4% and emulsifying agent 16.9%, surplus are deionized water.
10. a kind of preparation method of shin moisturizer as claimed in claim 9, it is characterised in that comprise the following steps successively：
S1) composition is added in deionized water, in 76 DEG C of heating stirring 1.6h, obtains mixture I；
S2) to step S1) NMF and emulsifying agent are added in the mixture I, in 62 DEG C of heating stirring 1.5h, obtained after cooling
Shin moisturizer.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711055966.5A CN107638375A (en) | 2017-10-31 | 2017-10-31 | A kind of the spot removal and skin care liquid and preparation method of the extract of stem cell containing blueberry |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711055966.5A CN107638375A (en) | 2017-10-31 | 2017-10-31 | A kind of the spot removal and skin care liquid and preparation method of the extract of stem cell containing blueberry |
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| CN107638375A true CN107638375A (en) | 2018-01-30 |
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|---|---|---|---|
| CN201711055966.5A Withdrawn CN107638375A (en) | 2017-10-31 | 2017-10-31 | A kind of the spot removal and skin care liquid and preparation method of the extract of stem cell containing blueberry |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2603634A (en) * | 2020-12-21 | 2022-08-10 | Floweressence Cgh Ltd | Flower essence-containing medicaments |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
-
2017
- 2017-10-31 CN CN201711055966.5A patent/CN107638375A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (1)
| Title |
|---|
| 王建新 等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2603634A (en) * | 2020-12-21 | 2022-08-10 | Floweressence Cgh Ltd | Flower essence-containing medicaments |
| GB2603634B (en) * | 2020-12-21 | 2023-11-15 | Floweressence Cgh Ltd | Flower essence-containing medicaments |
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Application publication date: 20180130 |