CN105055265A - Product capable of maintaining beauty and keeping young and preparation method of product - Google Patents
Product capable of maintaining beauty and keeping young and preparation method of product Download PDFInfo
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- CN105055265A CN105055265A CN201510430171.2A CN201510430171A CN105055265A CN 105055265 A CN105055265 A CN 105055265A CN 201510430171 A CN201510430171 A CN 201510430171A CN 105055265 A CN105055265 A CN 105055265A
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Abstract
The invention disclose a product capable of maintaining beauty and keeping young and a preparation method of the product, belonging to the field of biotechnology, medical cosmetology and cosmetics. The product is prepared by adding Chinese herb extracts into human stem cell extracts, wherein the human stem cell extracts contain 0.001-90% of human stem cell active ingredients. The product can be independently used, and also can be mixed with other cosmetics for use, so as to achieve the efficacies of maintaining beauty and keeping young. According to the preparation method, a filtering method and relevant technical means including low-temperature transportation, storage and the like are combined, the technology is simple, the reaction is mild, and the biological activity of active substances in the product and the stability of the effect of the product are guaranteed; the preparation method of the product is completely free of a chemical process, so that no toxic and harmful substances including heavy metal residues and preservatives are generated, the biosecurity is high, the market promotion and application prospects are good, and the prepared product has obvious effects of caring skin and maintaining beauty.
Description
Technical field
The present invention relates to beauty and skin care, be specifically related to a kind of looks improving and the skin nourishing product and preparation method thereof, belong to biotechnology, medical cosmetology and cosmetic field.
Background technology
Skin is made up of epidermis, corium and subcutaneous tissue etc.Wherein, skin corium organizational structure is the most complicated, forms primarily of connective tissue, both containing adipose cell, capillary vessel, again containing nerve, hair follicle etc., shallow thinner be papillary layer, what deep layer was thicker is lamina reticularis.Skin-nourishing is mainly from the blood capillary in skin corium, our nutrient substance of taking in i.e., by blood circulation, infiltrate from the capillary arteries skin corium, absorbed by the substrate blast cell in stratum basale through tissue fluid (main component is intercellular lipid).Substrate blast cell also absorbs from the oxygen in health, oidiospore cell thus form the metabolism of skin.The number of intercellular lipid is the key that nutrient substance is transported to substrate blast cell, is also the whether healthy key of application on human skin.Along with the increase at people's age, in skin, intercellular lipid reduces gradually, cell splitting rate slows down, and then have impact on the microcirculation of skin, substrate blast cell in epidermis better cannot absorb the nutrient substance from taking in our diet, causes mottle, wrinkle, the various skin problem such as aging.
Like to be beautiful for the objective reality be deteriorated gradually with age growth skin and people and thirst for the eternal pursuit of young state, various looks improving and the skin nourishing product is constantly developed in recent years.But, the effective ingredient of looks improving and the skin nourishing product mostly in the market is plant component extraction thing, or fine chemical product, and in order to improve the cosmetic result of product, most material harmful to skin containing lead, hydrargyrum, ethanol, antiseptic, hormone, spice etc.Consumer is when using this series products, and be often difficult to the metabolism promoting epidermis cell, skin quality improves limited; Sometimes even there is the problem such as mottle, allergy, the grievous injury health of consumer.
Stem cell extracting solution is the biological technical field derived product that recent years, new development was got up.In current disclosed patent, existing based on the application of stem cell extract in cosmetics.Such as, CN102068391B and CN100508948C.Although these cosmetics have promote the metabolic effect of epidermis cell, looks improving and the skin nourishing limited efficiency; And preparation method is too simple and easy, cell extract treatment step is few, and the probability of microbial contamination is comparatively large, therefore needs to improve its processing technology.
Summary of the invention
The object of this invention is to provide a kind of looks improving and the skin nourishing product.
Another object of the present invention is to provide the preparation method of above-mentioned looks improving and the skin nourishing product.
Looks improving and the skin nourishing product described in the present invention, containing activity in human stem cell composition and Chinese medicine extract.Activity in human stem cell composition comprises cell growth factor (cellgrowthfactor, CGF) isoreactivity material, these active substances can promote that epidermal stem cells is bred, and promote skin metabolism, and then reach effect of the skin maintenances such as whitening, wrinkle removal, reparation cicatrix.The Chinese medicine extract added on the one hand can nourish epidermal stem cells, in addition also Absorbable organic halogens Stem Cell Activity composition, makes it better play a role.The mechanism of action of this skin maintenance of this product just.
Looks improving and the skin nourishing product provided by the invention, by activity in human stem cell composition and Chinese medicine extract compatibility, this looks improving and the skin nourishing product is the Chinese medicine extract adding 20 ~ 50UG/ML in human stem cell extract; Be 0.001% ~ 90% containing activity in human stem cell composition quality percentage composition in described human stem cell extract, all the other sugar, albumen, vitamin and water required for activity in human stem cell composition homergy.
When described looks improving and the skin nourishing product is anti-aging product, anti-aging product is the Chinese medicine extract adding 20 ~ 50UG/ML in human stem cell extract.Described Chinese medicine extract is Radix Astragali extract, Radix Angelicae Sinensis extract and Folium Ginkgo extract, and between three, weight ratio meets 1:1:1.5.
Described activity in human stem cell composition is the active component extracted from one or more materials people's umbilical cord hematopoietic stem cell, umbilical cord mesenchymal stem cells, human amnion mesenchymal stem cell, mesenchymal stem cells MSCs, fat stem cell, neural stem cell or embryonic stem cell; Described Chinese medicine extract be Radix Astragali extract, Radix Angelicae Sinensis extract, Rhizoma Chuanxiong extract, Radix Notoginseng extract, Rhizoma Polygonati Odorati extract and Folium Ginkgo extract etc. have in the Chinese medicine of beauty functions any one or two or more.In described Chinese medicine extract, the mass percentage content of active component is more than 50%.Active component in described Chinese medicine extract comprise in glycoside, acid, polyphenol, polysaccharide, terpenoid, flavone and alkaloid one or more.
Above-mentioned looks improving and the skin nourishing product can be used alone, also can be used in combination with other cosmetics, has the effect of looks improving and the skin nourishing.
The preparation method of described a kind of looks improving and the skin nourishing product, comprises the following steps:
(1) acquisition neonatal umbilical cord mescenchymal stem cell is gathered: with the normal saline flushing neonatal umbilical cord tissue containing mycillin, be layered in Tissue Culture Dish after being cut into small pieces and cultivate;
(2) Tissue Culture Dish poly-D-lysine bag quilt, dries rear use naturally;
(3) carry out original cuiture with primary culture medium to by adherent cell, within every three days, change a cell culture fluid; Peptic cell, digestion end Secondary Culture base carries out termination digestion, goes down to posterity in adherent area 1:3 ratio;
(4) collecting cell culture fluid: collect the cell culture supernatant before going down to posterity, obtain cell culture fluid;
(5) cell culture fluid collected is through filtration system, and elimination cell debris and microorganism, obtain filtrate;
(6) to be placed in the super filter tube low-temperature and high-speed of 5K centrifugal for filtrate, the concentrated solution obtained; Temperature is 4 DEG C, and centrifugation rate is 2750rpm.
(7) gained concentrated solution mixes with Chinese medicine extract, stores under being placed in-20 DEG C of environment.
Looks improving and the skin nourishing product provided by the invention is rich in the bioactive substances such as cytokine, and these bioactive substance room temperature stability inferiors are poor, and changeableness inactivation, has a strong impact on its bioavailability, even loses the effect of looks improving and the skin nourishing; On the other hand, these active substances are easy to as nutrient substance the pollution being subject to microorganism.Therefore, the requirement of skin maintenance product of the present invention to production technology is higher: the biological function that should ensure the bioactive ingredients of product, avoids the contamination and deterioration of product again.The High Temperature High Pressure of abandoning tradition of the present invention, chemosterilization mode, adopt Filtration in conjunction with the related art method such as low-temperature transport, preservation, technique be simple, reaction temperature and, ensure that the biological activity of active substance in product and the stability of product effect.
The preparation method of looks improving and the skin nourishing product of the present invention departs from chemical process completely, not containing the poisonous and harmful substance such as heavy-metal residual, antiseptic, biological safety is high, marketing and application prospect good, the looks improving and the skin nourishing product obtained has effect of improving looks significantly.
Accompanying drawing explanation
Fig. 1 looks improving and the skin nourishing product provided by the invention is to human fibroblasts proliferative effect design sketch.
Fig. 2 A and Fig. 2 B is respectively the effect schematic diagram that experimenter uses product forward and backward.
Fig. 3 A and Fig. 3 B is respectively the effect schematic diagram that experimenter uses product forward and backward.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the invention will be further described.
Embodiment 1:
The present embodiment provides a kind of anti-aging product, and described anti-aging product comprises activity in human stem cell composition, obtains in the following way:
(1) acquisition neonatal umbilical cord mescenchymal stem cell is gathered: with the normal saline flushing neonatal umbilical cord tissue containing mycillin, be layered in Tissue Culture Dish after being cut into small pieces and cultivate;
(2) Tissue Culture Dish poly-D-lysine bag quilt, dries rear use naturally;
(3) carry out original cuiture with primary culture medium to by adherent cell, within every three days, change a cell culture fluid; Peptic cell, digestion end Secondary Culture base carries out termination digestion, goes down to posterity in adherent area 1:3 ratio;
(4) collecting cell culture fluid: collect the cell culture supernatant before going down to posterity, obtain cell culture fluid;
(5) cell culture fluid collected is through filtration system, and elimination cell debris and microorganism, obtain filtrate;
(6) to be placed in the super filter tube low-temperature and high-speed of 5K centrifugal for filtrate, the concentrated solution obtained; Temperature is 4 DEG C, and centrifugation rate is 2750rpm.
Be 0.001% ~ 90% containing activity in human stem cell composition quality percentage composition in described concentrated solution, all the other sugar, albumen, vitamin and water required for activity in human stem cell composition homergy.
(7) gained concentrated solution mixes with Chinese medicine extract, stores under being placed in-20 DEG C of environment, is the anti-aging product of the present embodiment.
In described anti-aging product, the concentration adding Chinese medicine extract is 50UG/ML, wherein containing Radix Astragali extract and each 20UG/ML of Radix Angelicae Sinensis extract, Folium Ginkgo extract 30UG/ML.
To take the logarithm the human fibroblasts of trophophase, after the trypsinization by 0.25% (quality is than concentration), with about 5 × 10
4the density of individual/mL is inoculated in 96 orifice plates, every hole 100 μ L.Adding RPMI1640 culture medium, is being 5%CO containing percent by volume
2incubator in 37 DEG C cultivate 12h, add anti-aging product of the present invention, continue cultivation and after 24 hours, add MTT (chemistry 3-(4 by name, 5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide bromine salt, trade name: tetrazolium bromide) reagent, add DMSO (dimethyl sulfoxide) after hatching 4 hours, measure under 570nm wavelength and absorb, experiment repetition 3 times.As shown in Figure 1, compared with blank group, product of the present invention, can promote the growth of human skin fibroblast to result, promotes that cell proliferation reaches more than one times.
Anti-aging product of the present invention, can promote the growth of human skin fibroblast, accelerates the metabolism of cell, can play slow down aging, repair skin maintenance effect of damaged skin.
Adopt the above-mentioned anti-aging product provided to carry out experimental test, experimenter amounts to 120 people, and stochastic averagina divides three groups: experimental group, blank group and placebo group.Experimental group: experimenter smears anti-aging product of the present invention 2 times every day sooner or later; Placebo group: experimenter smears pure water 2 times every day sooner or later; Blank group: experimenter is without any process.The effect of experimenter was once evaluated in every three days.Looks improving and the skin nourishing overall merit is divided into Three Estate, that is: 0-3 is divided into DeGrain; 4-6 is divided into effect general; 7-10 is divided into Be very effective.
After 30 days, general effect is marked, and result is as shown in table 1 below.
Table 1 result of the test
| Group | Scoring |
| SCN +Group (experimental group) | 8.5±0.9 |
| Blank group | 2.4±0.7 |
| Placebo group | 2.6±0.8 |
Result of the test shows: experimenter's looks improving and the skin nourishing overall merit grade is that 7-10 divides, and anti-wrinkle effect is remarkable.
Defying age embodiment: experimenter Zhang, female, 53 years old, before tested, canthus fishtail line was very dark, and eye pouch is very large, and bridge of the nose expression stricture of vagina is high-visible, as Fig. 2 A.From 15 days March in 2015, accept face use these cosmetics.Weekly, be a course for the treatment of for 5 times.Using flow process: the mediation of clean-exfoliation-micropin uses this product, and use product course for the treatment of (35 days), cosmetic result is remarkable: canthus fishtail line shoals/. eye pouch diminishes, and bridge of the nose expression stricture of vagina almost disappears, as shown in Figure 2 B.
Experimenter Wang, female, 57 years old.Before tested, forehead wrinkles on one's forehead are very dark, and canthus fishtail line is very dark, and eye pouch is very large, sees Fig. 3 A.From 15 days March in 2015, accept face use these cosmetics.Weekly, be a course for the treatment of for 5 times.Use flow process: the mediation of clean-exfoliation-micropin uses this product, and use product one and half course for the treatment of (45 days), cosmetic result is remarkable: forehead wrinkles on one's forehead shoal, and wrinkle shoals near the eyes, and eye pouch diminishes, and black eye are desalinated, as shown in Figure 3 B.
The above results shows, uses product of the present invention, and subcutaneous tissue absorbs infiltration, effectively can promote that the breeding of tissue stem cell is broken up, generate a large amount of newborn active epidermis cell, thus reach the effect of skin maintenance.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. a looks improving and the skin nourishing product, is characterized in that: this looks improving and the skin nourishing product is the Chinese medicine extract adding 20 ~ 50UG/ML in human stem cell extract; Be 0.001% ~ 90% containing activity in human stem cell composition quality percentage composition in described human stem cell extract, all the other sugar, albumen, vitamin and water required for activity in human stem cell composition homergy.
2. a kind of looks improving and the skin nourishing product according to claim 1, is characterized in that: described activity in human stem cell composition is the active component extracted from one or more materials people's umbilical cord hematopoietic stem cell, umbilical cord mesenchymal stem cells, human amnion mesenchymal stem cell, mesenchymal stem cells MSCs, fat stem cell, neural stem cell or embryonic stem cell; Described Chinese medicine extract be in Radix Astragali extract, Radix Angelicae Sinensis extract, Rhizoma Chuanxiong extract, Radix Notoginseng extract, Rhizoma Polygonati Odorati extract and Folium Ginkgo extract any one or two or more, in described Chinese medicine extract, the mass percentage content of active component is more than 50%.
3. a kind of looks improving and the skin nourishing product according to claim 1 and 2, is characterized in that: the active component in described Chinese medicine extract comprise in glycoside, acid, polyphenol, polysaccharide, terpenoid, flavone and alkaloid one or more.
4. a kind of looks improving and the skin nourishing product according to claim 1, it is characterized in that: described looks improving and the skin nourishing product is anti-aging product, activity in human stem cell composition is extract from human umbilical cord mesenchymal stem cells, Chinese medicine extract is Radix Astragali extract, Radix Angelicae Sinensis extract and Folium Ginkgo extract, and the quality ratio of three kinds of extracts is 1:1:1.5.
5. the preparation method of a kind of looks improving and the skin nourishing product according to claim 1, is characterized in that: comprise the following steps,
(1) acquisition neonatal umbilical cord mescenchymal stem cell is gathered: with the normal saline flushing neonatal umbilical cord tissue containing mycillin, be layered in Tissue Culture Dish after being cut into small pieces and cultivate;
(2) Tissue Culture Dish poly-D-lysine bag quilt, dries rear use naturally;
(3) carry out original cuiture with primary culture medium to by adherent stem cell, within every three days, change a cell culture fluid; Peptic cell, digestion end Secondary Culture base carries out termination digestion, goes down to posterity in adherent area 1:3 ratio;
(4) collecting cell culture fluid: collect the cell culture supernatant before going down to posterity, obtain cell culture fluid;
(5) cell culture fluid collected through filtration system, elimination cell debris and microorganism;
(6) to be placed in the super filter tube low-temperature and high-speed of 5K centrifugal for filtrate, obtains concentrated solution;
(7) gained concentrated solution mixes with Chinese medicine extract, stores under being placed in-20 DEG C of conditions, namely obtains described looks improving and the skin nourishing product.
6. an application for looks improving and the skin nourishing product, is characterized in that: described looks improving and the skin nourishing product is used alone, or used in combination with other cosmetics, has the effect of skin maintenance.
7. the application of looks improving and the skin nourishing product according to claim 6, it is characterized in that: other described cosmetics comprise clean class, skin type and beauty treatment class, and described skin type comprises emollient cream, cold cream, vanishing cream, emulsion, eye cream, hand cream, day cream, late frost, chapstick, floral water, shrinkage water and astringent; Described clean class comprises cleansing cream, Cleansing Foam, bath foam and shaving product; Described beauty treatment class comprises vanishing cream, XIANGFEN, facial film, lip pomade and kermes.
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Cited By (10)
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| CN106176530A (en) * | 2016-08-16 | 2016-12-07 | 韩荣成 | A kind of biological skin-protecting face nursing product and preparation method thereof |
| CN106389491A (en) * | 2016-09-30 | 2017-02-15 | 广州赛莱拉干细胞科技股份有限公司 | Composition and application thereof to preparation of product for improving chloasma |
| CN106730015A (en) * | 2017-01-06 | 2017-05-31 | 朱凯林 | A kind of preparation for beauty and preparation method thereof |
| CN107041894A (en) * | 2016-12-26 | 2017-08-15 | 湖南新起源医疗技术有限公司 | A kind of stem cell liquid for skin delication preparation method for anti-aging |
| CN107441128A (en) * | 2017-08-09 | 2017-12-08 | 广州赛莱拉干细胞科技股份有限公司 | A kind of pharmaceutical composition for promoting injured nerve regeneration and functional rehabilitation and its application and stem cell injection liquid and preparation method thereof |
| CN107468866A (en) * | 2017-09-11 | 2017-12-15 | 上海亚睿生物科技有限公司 | A kind of deep dermis regenerative agent being mixed with using stem cell extract and Chinese medical extract and its preparation method and application |
| CN107550934A (en) * | 2017-09-11 | 2018-01-09 | 上海亚睿生物科技有限公司 | A kind of skin-rehabilitating agent being mixed with using stem cell extract and Chinese medical extract and its application |
| CN107982197A (en) * | 2017-11-29 | 2018-05-04 | 中南大学 | Composition and its application containing non-stem cell constituents |
| CN108158933A (en) * | 2017-11-29 | 2018-06-15 | 中南大学 | Composition and its application containing non-stem cell constituents |
| CN113197920A (en) * | 2021-05-19 | 2021-08-03 | 杭州露源生物科技有限公司 | Preparation method of embryonic stem cell culture extract for skin aging resistance |
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| CN106176530A (en) * | 2016-08-16 | 2016-12-07 | 韩荣成 | A kind of biological skin-protecting face nursing product and preparation method thereof |
| CN106389491A (en) * | 2016-09-30 | 2017-02-15 | 广州赛莱拉干细胞科技股份有限公司 | Composition and application thereof to preparation of product for improving chloasma |
| CN107041894A (en) * | 2016-12-26 | 2017-08-15 | 湖南新起源医疗技术有限公司 | A kind of stem cell liquid for skin delication preparation method for anti-aging |
| CN106730015A (en) * | 2017-01-06 | 2017-05-31 | 朱凯林 | A kind of preparation for beauty and preparation method thereof |
| CN107441128A (en) * | 2017-08-09 | 2017-12-08 | 广州赛莱拉干细胞科技股份有限公司 | A kind of pharmaceutical composition for promoting injured nerve regeneration and functional rehabilitation and its application and stem cell injection liquid and preparation method thereof |
| CN107468866A (en) * | 2017-09-11 | 2017-12-15 | 上海亚睿生物科技有限公司 | A kind of deep dermis regenerative agent being mixed with using stem cell extract and Chinese medical extract and its preparation method and application |
| CN107550934A (en) * | 2017-09-11 | 2018-01-09 | 上海亚睿生物科技有限公司 | A kind of skin-rehabilitating agent being mixed with using stem cell extract and Chinese medical extract and its application |
| CN107468866B (en) * | 2017-09-11 | 2020-10-16 | 上海亚睿生物科技有限公司 | Deep skin regenerating agent prepared by mixing stem cell extract and traditional Chinese medicine extract, and preparation method and application thereof |
| CN107550934B (en) * | 2017-09-11 | 2020-12-29 | 上海亚睿生物科技有限公司 | Skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof |
| CN107982197A (en) * | 2017-11-29 | 2018-05-04 | 中南大学 | Composition and its application containing non-stem cell constituents |
| CN108158933A (en) * | 2017-11-29 | 2018-06-15 | 中南大学 | Composition and its application containing non-stem cell constituents |
| CN113197920A (en) * | 2021-05-19 | 2021-08-03 | 杭州露源生物科技有限公司 | Preparation method of embryonic stem cell culture extract for skin aging resistance |
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Application publication date: 20151118 |
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| RJ01 | Rejection of invention patent application after publication |