CN107636166A - 高度并行准确测量核酸的方法 - Google Patents

高度并行准确测量核酸的方法 Download PDF

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Publication number
CN107636166A
CN107636166A CN201680021886.5A CN201680021886A CN107636166A CN 107636166 A CN107636166 A CN 107636166A CN 201680021886 A CN201680021886 A CN 201680021886A CN 107636166 A CN107636166 A CN 107636166A
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China
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primers
pcr
dna
rna
specific
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CN201680021886.5A
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Chinese (zh)
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班纳吉·阿吉特·帕特尔
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201680021886.5A 2015-02-13 2016-02-14 高度并行准确测量核酸的方法 Pending CN107636166A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562116302P 2015-02-13 2015-02-13
US62/116,302 2015-02-13
US201562135923P 2015-03-20 2015-03-20
US62/135,923 2015-03-20
PCT/US2016/017920 WO2016131030A1 (en) 2015-02-13 2016-02-14 Methods for highly parallel and accurate measurement of nucleic acids

Publications (1)

Publication Number Publication Date
CN107636166A true CN107636166A (zh) 2018-01-26

Family

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CN201680021886.5A Pending CN107636166A (zh) 2015-02-13 2016-02-14 高度并行准确测量核酸的方法

Country Status (7)

Country Link
US (1) US20180010176A1 (https=)
EP (1) EP3256607B1 (https=)
JP (1) JP2018509178A (https=)
CN (1) CN107636166A (https=)
CA (1) CA2974398A1 (https=)
RU (1) RU2017131622A (https=)
WO (1) WO2016131030A1 (https=)

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CN112592968A (zh) * 2020-12-27 2021-04-02 苏州科诺医学检验实验室有限公司 高通量测序用分子标签接头及其合成方法与应用

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US11371087B2 (en) * 2016-06-10 2022-06-28 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
EP3601593B1 (en) * 2017-03-24 2021-12-22 Bio-Rad Laboratories, Inc. Universal hairpin primers
CN106835292B (zh) * 2017-04-05 2019-04-09 北京泛生子基因科技有限公司 一步法快速构建扩增子文库的方法
DE202019005627U1 (de) 2018-04-02 2021-05-31 Grail, Inc. Methylierungsmarker und gezielte Methylierungssondenpanels
EP3833776A4 (en) * 2018-08-06 2022-04-27 Billiontoone, Inc. DILUTION MARKER FOR QUANTIFICATION OF BIOLOGICAL TARGETS
WO2020069350A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
BR112021018125A2 (pt) 2019-10-25 2021-11-16 Illumina Cambridge Ltd Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado
US20230033987A1 (en) * 2019-12-16 2023-02-02 Cargill, Incorporated Isolated oleosome composition and process for preparing it
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
WO2021195277A1 (en) * 2020-03-24 2021-09-30 President And Fellows Of Harvard College Multiplexed methods for detecting target rnas
JP2023524016A (ja) * 2020-04-29 2023-06-08 フリーノム ホールディングス，インク． 結腸細胞増殖性障害を特定するためのｒｎａマーカと方法
US10941453B1 (en) * 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
WO2021262422A1 (en) * 2020-06-25 2021-12-30 Huang Chung Ying Method and system of dna and rna detection
CN115125295B (zh) * 2022-03-10 2025-03-14 安徽师范大学 一种用于多位点可持续使用的基因分型标准品
KR20250019610A (ko) 2022-03-21 2025-02-10 빌리언투원, 인크. 치료 모니터링을 위한 메틸화된 세포 유리 dna의 분자 계수
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing

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WO2012112804A1 (en) * 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
WO2013036929A1 (en) * 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior Methods for obtaining a sequence
CN103717749A (zh) * 2011-04-25 2014-04-09 伯乐生命医学产品有限公司 用于核酸分析的方法和组合物
CN107532202A (zh) * 2015-02-24 2018-01-02 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法

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US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US7270983B1 (en) * 2004-02-19 2007-09-18 Research Foundation Of The University Of Central Florida, Inc. Messenger RNA profiling: body fluid identification using multiplex reverse transcription-polymerase chain reaction (RT-PCR)
US20060019258A1 (en) * 2004-07-20 2006-01-26 Illumina, Inc. Methods and compositions for detection of small interfering RNA and micro-RNA
JPWO2007141912A1 (ja) * 2006-06-07 2009-10-15 住友ベークライト株式会社 Ｒｎａ検出方法
JP5299986B2 (ja) * 2007-11-01 2013-09-25 国立大学法人山口大学 核酸の定量方法
AU2013267609C1 (en) * 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
KR20200140929A (ko) * 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
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Publication number Priority date Publication date Assignee Title
WO2012112804A1 (en) * 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
CN103717749A (zh) * 2011-04-25 2014-04-09 伯乐生命医学产品有限公司 用于核酸分析的方法和组合物
WO2013036929A1 (en) * 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior Methods for obtaining a sequence
CN107532202A (zh) * 2015-02-24 2018-01-02 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112592968A (zh) * 2020-12-27 2021-04-02 苏州科诺医学检验实验室有限公司 高通量测序用分子标签接头及其合成方法与应用

Also Published As

Publication number Publication date
RU2017131622A3 (https=) 2019-10-25
EP3256607A1 (en) 2017-12-20
EP3256607A4 (en) 2019-03-13
RU2017131622A (ru) 2019-03-13
WO2016131030A4 (en) 2016-10-20
EP3256607B1 (en) 2021-01-20
WO2016131030A1 (en) 2016-08-18
US20180010176A1 (en) 2018-01-11
JP2018509178A (ja) 2018-04-05
CA2974398A1 (en) 2016-08-18

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Application publication date: 20180126